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1. Effect of the Application of Gluconacetobacter diazotrophicus and Its Interaction with Nitrogen and Phosphorus Fertilization on Carrot Yield in the Field

Author

Nelson Ceballos-Aguirre, Jorge Andrés Cuellar, Gloria María Restrepo, and Óscar Julián Sánchez

Subjects
Agriculture (General), S1-972
Abstract

Carrot production is expensive since approximately 51% of the total costs are allocated to the nutrition of the crop. Bacterial inoculants are a promising alternative for crop fertilization. This research aimed to evaluate the effect of Gluconacetobacter diazotrophicus on the performance of carrot cultivar “Royal Chantenay” and its interaction with nitrogen and phosphorus fertilization. An experimental design of sub-subdivided plots was applied, where the largest plot consisted of the reference strain (ATCC 49037) and a native Colombian isolate of the bacterium (GIBI029); two concentrations of the bacterium were applied in the subplots (8.8 × 107 and 18 × 107 CFU/mL), and the levels of nitrogen and phosphorus were sorted within each one of them. The best positive effect was observed with the application of G. diazotrophicus ATCC49037 and GIBI029 at a concentration of 18 × 107 CFU/mL without the application of phosphorus or nitrogen in which yields of 37,417 and 30,606 kg/ha were obtained, respectively, exceeding the national average production in Colombia. In contrast, conventional treatment had a yield of 27,909 kg/ha. Additionally, higher quality was evidenced in the product weight with values of 126.48 g (ATCC49037) and 104.98 g (GIBI029), compared with the conventional treatment (93.19 g). G. diazotrophicus was shown to exhibit growth-promoting properties not only in crops such as sugarcane but also in economically important vegetable crops. The results obtained may contribute to the development of a novel microbial inoculant for vegetables under agroecological conditions in tropical areas.

Published
2023
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2. Economic feasibility of Gluconacetobacter diazotrophicus in carrot cultivation

Author

Nelson Ceballos-Aguirre, Gloria María Restrepo, Alejandro Hurtado-Salazar, Jorge Andrés Cuellar, and Óscar Julián Sánchez

Subjects
diazotrophic bacteria, plant growth promoter, benefit / cost ratio, Agriculture (General), S1-972, Biotechnology, TP248.13-248.65
Abstract

ABSTRACT The inclusion of more sustainable alternatives such as bacterial inoculants is a viable option for the competitiveness of vegetable crops in tropical countries such as Colombia. The economic feasibility of a bacterial suspension of G. diazotrophicus applied to the carrot crop was determined. The native isolate G. diazotrophicus GIBI029 was evaluated and the strain ATCC 49037 was used as a control. The experiment was installed in a subdivided plot design, where the plot was the bacterium G. diazotrophicus (ATCC49037 and GIBI029. The subplot was the concentration of G. diazotrophicus (88×106 CFU/mL and 18×107 CFU/mL) and, in it, the levels of nitrogen and phosphorus (0% and 100% nitrogen and phosphorus) were assorted. The average weight of the carrot (g) and the yield by quality of the consuming organ (kg/ha) were evaluated. Through the production cycle, fixed, variable, and total costs were calculated. Benefit / cost ratios higher than 1.46 and net income up to US$ 10,817/ha were achieved. It is possible to efficiently and economically use the native isolate G. diazotrophicus GIBI029 in the search for more sustainable and competitive cultural practices.

Published
2022
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3. Actividades ligninolíticas y producción de basidiomas de Ganoderma lucidum en cultivo sólido suplementado con manganeso

Author

Sandra Montoya Barreto, Gloria María Restrepo Franco, and Claudia Lorena Calvo Rodríguez

Subjects
eficiencia biológica, ligninasas, fructificación, sustratos suplementados con sulfato de manganeso II, Biotechnology, TP248.13-248.65
Abstract

Ganoderma lucidum es un basidiomiceto de pudrición blanca estudiado especialmente por sus atributos medicinales. No obstante, la información sobre la suplementación de los medios de cultivo con metales como Zn, Li, Mn, Cu es escasa aun conociendo que la presencia de metales en los sustratos mejora las características de los basidiomas obtenidos e incrementa su productividad. El objetivo fue evaluar las actividades enzimáticas lacasa (Lc) y manganeso peroxidasa (MnP), la fructificación y eficiencia biológica (EB) de G. lucidum en cultivos sólidos formulados con residuos agroindustriales (aserrín de roble, cascarilla de café, salvado de maíz) suplementados con dos niveles de sal de manganeso II (0,05 % y 0,1 % p/p) y una formulación sin adición de la sal de manganeso. Las actividades enzimáticas se determinaron durante 98 días del ciclo productivo, con toma de muestras semanales. El tratamiento uno (0,05 % p/p de MnSO4.H2O) suministró la mayor EB del cultivo con 25,90 ± 2,12 % y los mayores títulos de actividades ligninolíticas en el tiempo con 0,7299 UE/g s.s. de MnP a los 35 días de fermentación y 4,1760 UE/g s.s para la actividad de Lc a los 42 días de proceso con relación a los tratamientos dos y control. Asimismo, hubo una disminución del ciclo de cultivo del hongo para los tratamientos uno (83 días) y dos (95 días) en comparación con el tratamiento control (117 días). Los resultados de este trabajo son promisorios para cultivadores industriales de G. lucidum, ya que la suplementación de los sustratos con Mn incrementa la productividad de los cultivos.

Published
2022
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4. Antagonism of plant growth promoting rhizobacteria against the causal agent of the vascular wilting of tomato

Author

Narmer Fernando Galeano Vanegas, Sandra Maria Marulanda Moreno, Beatriz Elena Padilla Hurtado, Javier Guillermo Mantilla Afanador, Nelson Ceballos Aguirre, and Gloria María Restrepo Franco

Subjects
Inhibition of germination, chitinase activity, endoglucanase activity, dual tests., Biotechnology, TP248.13-248.65
Abstract

Use of biotechnological potential of native microorganisms as bio-inputs is having a great impact on agricultural systems. Plant Growth Promoting Rhizobacteria (PGPR), in addition to their beneficial effect on plant growth and on the availability of soil elements, also have an antagonistic effect against different pathogens. In this study, growth promotion mechanisms with emphasis on the antagonism of PGPR isolated from sugarcane and tomato crops were evaluated. Antagonism against Fusarium oxysporum f.sp lycopersici (Fol) was determined by dual tests, inhibition of germination and production of chitinases and endoglucanases. 52 isolates were evaluated and according to their results in dual tests 10 were selected for further analysis. Isolate GIBI127 showed the best percentage of Inhibition Germination (IG) of Fol (59.29%). Then, a selection index was calculated using results from gi, dual tests and growth promotion mechanisms to select five best isolates. Finally, these bacteria were evaluated for chitinases and endoglucanases production using Miller´s method. As a result, strain GIBI419 (Burkholderia cepacia) showed a higher production of these enzymes. Selected isolates have antagonistic potential along with plant growth promotion characteristics, which can be used for the development of microbial inoculants which allow the establishment of agricultural systems for tomato cultivation that are sustainable, efficient, and environmentally friendly.

Published
2020
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5. Bacterias diazotróficas con actividad promotora del crecimiento vegetal en Daucus carota L.

Author

Jennifer Gaviria-Giraldo, Gloria María Restrepo-Franco, Narmer Fernando Galeano-Vanegas, and Annia Hernández-Rodríguez

Subjects
actividad nitrogenasa, fijación de nitrógeno, solubilización de fosfato, zanahoria, Agriculture, Agriculture (General), S1-972
Abstract

Este trabajo se propuso aislar bacterias diazotróficas con actividad promotora del crecimiento vegetal, asociadas al cultivo de zanahoria. Se realizaron 3 muestreos, a los 30, 60 y 115 días, en una granja ubicada en una zona rural del municipio de Manizales (Caldas). El aislamiento de las bacterias diazotróficas se efectuó en medios de cultivo semisólidos libres de nitrógeno. A los aislamientos obtenidos se les realizó descripción macro y microscópica, identificación bioquímica y molecular. Se evaluaron características como determinación de compuestos indólicos, actividad nitrogenasa y solubilización de fosfatos. Como cepa patrón se empleó Gluconacetobacter diazotrophicus ATCC 49037. Se recuperaron 20 aislamientos asociados a la rizosfera y 12 al rizoplano. La identificación molecular mostró cinco géneros presentes: Rhizobium, Achromobacter, Bacillus, Enterobacter y Stenotrophomonas. La producción de compuestos indólicos presentó concentraciones entre 9,73 y 112,8 µg/mL. La cepa patrón presentó una actividad mayor, con una producción de compuestos indólicos de 172,5 µg/mL. En la actividad nitrogenasa los aislamientos GIBI411, 394 y 399 tuvieron una actividad mayor o similar a la cepa patrón. Los aislamientos más eficientes en la solubilización de fosfato tricálcico fueron GIBI378 y 385. La solubilización de fosfato de aluminio se valoró por el índice de producción de ácido, siendo los aislamientos GIBI378, 391, 387 y 388 los de mejor comportamiento en esta variable. Los aislamientos encontrados son candidatos potenciales para desarrollar nuevos procesos biotecnológicos para la producción de nuevos biofertilizantes alternativos, considerando las importantes propiedades de promoción del crecimiento vegetal determinadas en este trabajo.

Published
2018
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6. An Improved Robust Adaptive Controller for a Fed-Batch Bioreactor with Input Saturation and Unknown Varying Control Gain via Dead-Zone Quadratic Forms

Author

Alejandro Rincón, Gloria María Restrepo, and Óscar J. Sánchez

Subjects
adaptive control, augmented error signal, fed-batch bioreactor, input saturation, Lyapunov-like function, Electronic computers. Computer science, QA75.5-76.95
Abstract

In this work, a new adaptive controller is designed for substrate control of a fed-batch bioreactor in the presence of input saturation and unknown varying control gain with unknown upper and lower bounds. The output measurement noise and the unknown varying nature of reaction rate and biomass concentration and water volume are also handled. The design is based on dead zone quadratic forms. The designed controller ensures the convergence of the modified tracking error and the boundedness of the updated parameters. As the first distinctive feature, a new robust adaptive auxiliary system is proposed in order to tackle input saturation and control gain uncertainty. As the second distinctive feature, the modified tracking error converges to a compact region whose bound is user-defined, in contrast to related studies where the convergence region depends on upper bounds of either external disturbances, system states, model parameters or terms and model parameter values. Simulations confirm the properties of the closed loop behavior.

Published
2021
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7. Evaluación de un sistema de clasificación hidráulica para separación diferencial de mezclas sólidas

Author

Adriana Marcela Osorio Correa, Juan Miguel Marín, Juan Felipe Peláez Restrepo, and Gloria María Restrepo Vásquez

Subjects
hydraulic classification, differential separation, elutriation, solid mixture, Engineering (General). Civil engineering (General), TA1-2040
Abstract

Se evaluó un sistema de clasificación hidráulica para separación diferencial de mezclas sólidas en 4 puntos de descarga equi- distantes, mediante un diseño factorial 23 para cada una de estas descargas, siendo los factores a evaluar el caudal en 0,9 y 2,7 m3/h, la densidad en 1.420 y 2.520 kg/m3 y el tamaño de partícula en 0,0005 y 0,002 m, la variable de respuesta fue el grado de recuperación de cada material; los modelos obtenidos mediante el software estadístico Stat-Graphics presentaron correlacio- nes mayores del 99%, los modelos se validaron experimentalmente en dos casos: primero, clasificación de un material en diver- sos tamaños e igual densidad, y segundo, separación de dos materiales de igual tamaño pero diferente densidad. Los materia- les usados fueron carbón y cuarzo; estos se caracterizaron en término de sus propiedades como partículas; individuales y como masa de partículas, la separación por tamaños se hizo mediante análisis granulométricos por tamizado. Los porcentajes de error en el primer caso resultaron ser bajos, entre 0,7 y 14%, sin embargo, para el segundo caso los errores en la predicción de la separación del material más pesado resultaron muy elevados (superiores a 50%), debido tal vez a la naturaleza del material. Fi- nalmente, los modelos encontrados se constituirán como una base para seguir estudiando aquellas variables de operación y di- seño que en la práctica determinan la respuesta dinámica y el rendimiento operacional de estos procesos, para la búsqueda de condiciones óptimas de operación.

Published
2009

9. Tecnología Biofloc: Perspectivas de Aplicación en Procesos de Producción Piscícola Sostenible

Author

Gloria María Restrepo Franco, Eduardo Javid Corpas Iguarán, and Johana Gaitán Sánchez

Subjects
Multidisciplinary
Abstract

Introducción: la tecnología Biofloc, es un proceso de biorremediación que permite minimizar el intercambio de agua y su uso en los sistemas de acuicultura. Objetivo: presentar tendencias de las publicaciones científicas acerca de la tecnología Biofloc para producción piscícola, utilizando las herramientas de análisis bibliométrico. Materiales y métodos: se empleó la base de datos Web of Science, con una ecuación de búsqueda y procesamiento de la información apoyada por las herramientas BibExcel y VOSviewer. Se realizó un análisis sistemático en los documentos recuperados, teniendo en cuenta: tipo de cultivo, tiempo experimental, rendimiento de crecimiento y proteico, densidad poblacional, calidad del agua, características microbiológicas y fisiológicas del cultivo. Se determinaron categorías en las cuales están definidos los estudios con relación a la tecnología y los principales desafíos identificados en la aplicación de la tecnología. Resultados: se encontraron 258 documentos, el 94,12 % correspondieron a artículos de investigación. La productividad fue mayor en 2014 y 2016. La Universidad Federal de Santa Catarina y la Universidad Federal de Rio Grande FURG en Brasil tienen la mayor producción. En coautorías se identificaron 18 nodos. El 59,3 % de los documentos reportaron investigaciones con camarón y el 18,99 % con tilapia. Los mejores tiempos de producción estuvieron en los rangos de 41-50 días (12,4 %), 21-30 días (11,24 %) y 51-60 días (9,68 %). La relación C: N no fue reportada en el 92,6 % de los trabajos. Sin embargo, el 3,48 % reportaron relaciones de 15:1 y el 1,16 % de 20:1. Las principales categorías fueron rendimiento (44,18 %) y -Revista Lasallista de Investigación- Vol. 19 No. 1 / enero-junio-2022 Tecnología Biofloc: Perspectivas de Aplicación en Procesos de Producción Piscícola Sostenible 166 Johana Gaitán Sánchez, Eduardo Javid Corpas Iguarán, Gloria María Restrepo Franco productividad (26,35 %). Conclusiones: la tecnología Biofloc representa una oportunidad para la sustentabilidad en prácticas acuícolas de especies. Se evidenció un crecimiento gradual en los procesos de investigación en los últimos años; los principales estudios están relacionados con el rendimiento en cuanto a crecimiento y optimización de los procesos de conversión proteica en los cultivos analizados bien sean individuales o en policultivo.

Published
2022

10. Evaluación ambiental del efecto del uso de aditivos químicos en la estabilización de suelos viales

Author

Dairo León Vanegas Taborda, Gloria María Restrepo Vásquez, and Eliana Patricia Llano Cardona

Abstract

Las obras de infraestructura tales como caminos, carreteras y similares, proporcionan beneficios sociales y fortalecen las economías regionales. Sin embargo, al utilizar grandes áreas de los territorios y recursos naturales como fuente de materiales, causan en el entorno afectaciones que generan impactos ambientales importantes. Las tecnologías de estabilización química son soluciones alternativas a la pavimentación, mediante las cuales es posible mejorar las propiedades ingenieriles de los suelos para obtener adecuados desempeños mecánicos.El objetivo de este trabajo fue evaluar a nivel de laboratorio los impactos ambientales generados por el uso de químicos en la estabilización de suelos para aplicaciones viales, para ello se utilizó un suelo arcilloso para su aditivación con productos químicos de diferente naturaleza; se elaboraron probetas cilíndricas con el suelo aditivado compactadas bajo las condiciones obtenidas en el ensayo de compactación Proctor normal. Luego se sometieron a múltiples ensayos como pH, COT, DBO, DQO, biodegradabilidad y pruebas indirectas de generación de material particulado. Los resultados obtenidos evidenciaron que en general los aditivos empleados como estabilizantes químicos no presentan peligrosidad al ambiente en términos de toxicidad, biodegradabilidad y generación de material particulado.

Published
2022

11. Diversidad de macroinvertebrados acuáticos en dos ecosistemas lóticos en El Doncello, Caquetá

Author

Lalo Rodrigo Bravo Chaves and Gloria María Restrepo Franco

Subjects
General Medicine
Abstract

Estudiar macroinvertebrados acuáticos es útil para la estimación de la integridad de un ecosistema, debido a su capacidad de reflejar el efecto de una gran variedad de contaminantes. La investigación se realizó para evaluar la diversidad de macroinvertebrados acuáticos, presentes en dos ecosistemas lóticos del municipio de El Doncello, Caquetá, entre agosto de 2019 y marzo de 2020, comprendiendo periodos de aguas altas, transición y aguas bajas. Se establecieron tres estaciones de muestreo en el río Doncello y la quebrada Anayá del mismo municipio. Las colectas se realizaron con una red tipo D, empleando el método de muestreo intensivo en los diferentes microhábitats encontrados. La información se analizó con los paquetes estadísticos past y EstimateS para índices de diversidad y representatividad del muestreo, respectivamente. Se identificaron 95 géneros, 56 familias y 15 órdenes. Chironomidae (Diptera) fue la familia más abundante, seguida por Philopotamidae (Trichoptera) y Leptophlebiidae (Ephemeroptera). Los índices bióticos de calidad BMWP, ASPT y EPT permitieron evidenciar la influencia de la contaminación por residuos orgánicos de origen doméstico y la capacidad de autodepuración del agua de la quebrada Anayá. La comunidad de macroinvertebrados reportados responde a las perturbaciones antrópicas en los ecosistemas estudiados; las estaciones menos intervenidas presentaron la mayor riqueza, mientras que las más afectadas presentaron baja diversidad y alta dominancia.

Published
2021

12. Design and Evaluation of a Robust Observer Using Dead-Zone Lyapunov Functions—Application to Reaction Rate Estimation in Bioprocesses

Author

Alejandro Rincón Santamaría, Fredy E. Hoyos, and Gloria María Restrepo Franco

Subjects
state estimation, nonlinear observer design, nonlinear systems, bioprocess monitoring, software sensor, Plant Science, Biochemistry, Genetics and Molecular Biology (miscellaneous), Food Science
Abstract

This paper addresses the design and evaluation of a robust observer for second order bioprocesses considering unknown bounded disturbance terms and uncertainty in the dynamics of the unknown and known states. The observer design and the stability analysis are based on dead-zone Lyapunov functions, and a detailed procedure is provided. The transient response bounds and the convergence region of the unknown observer error are determined in terms of the disturbance bounds, considering persistent but bounded disturbances in the dynamics of both the known and unknown observer errors. This is a significant contribution to closely related observer design studies, in which the transient response bounds are determined, but persistent and bounded disturbances are not considered in the dynamics of the known observer error. Other important contributions are: (i) the procedure for defining the observer parameters is significantly simpler than common observer designs, since a solution to the Ricatti equation, solution to LMI constraints, or the accomplishment of eigenvalue inequality conditions are not required; (ii) discontinuous signals are not used in the observer; and (iii) the effect of the gain sign associated with the unknown state in the dynamics of the known state is explicitly and clearly considered in the observer design and in the convergence study. In addition, the guidelines for selecting the observer parameters are provided. Numerical simulation confirms the stability analysis results: the observer errors converge within a short time, with a low estimation error, if observer-parameters are properly defined.

Published
2022
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14. Antagonism of plant growth promoting rhizobacteria against the causal agent of the vascular wilting of tomato

Author

Nelson Ceballos Aguirre, Javier Guillermo Mantilla Afanador, Beatriz Elena Padilla Hurtado, Gloria María Restrepo Franco, Sandra María Marulanda Moreno, and Narmer Fernando Galeano Vanegas

Subjects
dual tests, 0106 biological sciences, 0301 basic medicine, biology, endoglucanase activity, food and beverages, Wilting, General Medicine, biology.organism_classification, Rhizobacteria, Inhibition of germination, 01 natural sciences, chitinase activity, 03 medical and health sciences, Horticulture, 030104 developmental biology, Burkholderia, Germination, Fusarium oxysporum, Antagonism, Microbial inoculant, TP248.13-248.65, Bacteria, Biotechnology, 010606 plant biology & botany
Abstract

Use of biotechnological potential of native microorganisms as bio-inputs is having a great impact on agricultural systems. Plant Growth Promoting Rhizobacteria (PGPR), in addition to their beneficial effect on plant growth and on the availability of soil elements, also have an antagonistic effect against different pathogens. In this study, growth promotion mechanisms with emphasis on the antagonism of PGPR isolated from sugarcane and tomato crops were evaluated. Antagonism against Fusarium oxysporum f.sp lycopersici (Fol) was determined by dual tests, inhibition of germination and production of chitinases and endoglucanases. 52 isolates were evaluated and according to their results in dual tests 10 were selected for further analysis. Isolate GIBI127 showed the best percentage of Inhibition Germination (IG) of Fol (59.29%). Then, a selection index was calculated using results from gi, dual tests and growth promotion mechanisms to select five best isolates. Finally, these bacteria were evaluated for chitinases and endoglucanases production using Miller´s method. As a result, strain GIBI419 (Burkholderia cepacia) showed a higher production of these enzymes. Selected isolates have antagonistic potential along with plant growth promotion characteristics, which can be used for the development of microbial inoculants which allow the establishment of agricultural systems for tomato cultivation that are sustainable, efficient, and environmentally friendly.

Published
2020

15. Modification and Expression of Beta-1,4-Endoglucanase encoding sequences of fungal origin in Escherichia coli BL21

Author

Narmer Fernando Galeano Vanegas, Gloria María Restrepo Franco, and Natalia Gutierrez Calle

Subjects
Cellulase, medicine.disease_cause, 03 medical and health sciences, Plasmid, Aspergillus nidulans, medicine, Lignocellulolytic enzymes, Cellulose, Escherichia coli, Gene, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, biology, 030306 microbiology, Chemistry, heterologous expression, Active site, General Medicine, biology.organism_classification, Enzyme assay, Enzyme, Biochemistry, biology.protein, bacteria, TP248.13-248.65, Biotechnology
Abstract

Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol production, promoting the bioeconomy. The objective of the present work was to express lignocellulolytic enzymes of eukaryotic origin in Escherichia coli BL21 (DE3). Initially, endoglucanase eukaryotic genes were selected and modified using bioinformatics methods for their production in E. coli BL21 (DE3) and saccharification of pure cellulose substrates. The gene selected for its modification and expression was eglB from the fungus Aspergillus nidulans. Subsequently the enzyme integrity was tested by 3D modeling and molecular docking, as well as the conformation of its active site and its affinity for substrates of interest. Finally, cloning of the modified gene in plasmid pET151 TOPO was made and transformed in the strain E. coli BL21 (DE3) where several lignocellulose degradation tests were carried out using semiquantitative methods for the enzyme activity in carboxymethylcellulose. The presence of the three genes of interest within the plasmid pET151 TOPO and within the transformed cells of E. coli TOP10 and E. coli BL21 (DE3) was verified by colony PCRs performed. The presence of this gen was corroborated by sequencing. Expression of the modified endoglucanase enzyme was achieved in E. coli BL21 (DE3) expression cells, in soluble and functional form, demonstrated by the hydrolysis of the CMC substrate.

Published
2020

16. Evaluación de la remoción de cianuro y metales pesados en efluentes líquidos provenientes del beneficio de oro de la pequeña minería, mediante adsorción con carbón activado y peróxido de hidrógeno en Segovia, Antioquia

Author

Narmer Fernando Galeano Vanegas, Claudia Catalina Estrada-Montoya, and Gloria María Restrepo Franco

Subjects
contaminación ambiental, Cyanide, remoción metales pesados, chemistry.chemical_element, 02 engineering and technology, Zinc, 010501 environmental sciences, 01 natural sciences, Peroxide, gold mining, heavy metals precipitation, chemistry.chemical_compound, Adsorption, medicine, heavy metals removal, Hydrogen peroxide, Effluent, 0105 earth and related environmental sciences, General Engineering, 021001 nanoscience & nanotechnology, cyanide degradation, chemistry, degradación de cianuro, minería de oro, environmental pollution, 0210 nano-technology, Carbon, Activated carbon, medicine.drug, Nuclear chemistry, precipitación de metales pesados
Abstract

The small gold mining generates toxic substances discharges, being an environmental problem. The objective was to evaluate the removal of cyanide and heavy metals, in liquid effluents from the gold benefit, by adsorption with activated carbon and hydrogen peroxide. The residues were first treated with carbon to determine the adsorption efficiency with 20, 40, 60 g of carbon / L of solution at times of 4, 8, 12 hours. Then hydrogen peroxide (1.0, 1.5, 2.0 liters of peroxide / Kg CN in solution, was added over 4 hours). The response variables were concentrations of cyanide, lead, zinc, iron. The best treatment with carbon was 60 g of carbon / L of solution and 12 hours of contact and for the process with hydrogen peroxide: 2 liters of H2O2 / Kg of CN in solution, during 4 hours. A flow chart and tables for the implementation of the process were designed. Resumen La pequeña minería de oro genera vertimientos de sustancias tóxicas, siendo una problemática ambiental. El objetivo fue evaluar la remoción de cianuro y metales pesados, en efluentes líquidos del beneficio de oro, mediante la adsorción con carbón activado y peróxido de hidrógeno. Los residuos primero se trataron con carbón para determinar la eficiencia de adsorción con 20, 40, 60 g de carbón/L de solución a tiempos de 4, 8, 12 horas. Luego se adicionó peróxido de hidrógeno (1,0, 1,5, 2,0 litros de peróxido/Kg CN en solución, durante 4 horas). Las variables de respuesta fueron concentraciones de cianuro, plomo, zinc, hierro. El mejor tratamiento con carbón fue 60 g de carbón/L de solución y 12 horas de contacto y para el proceso con peróxido de hidrógeno: 2 litros de H2O2/Kg de CN en solución, durante 4 horas. Se diseñó un diagrama de flujo y tablas para la implementación del proceso.

Published
2020

17. Wastewater treatment from biodiesel production via a coupled photo-Fenton–aerobic sequential batch reactor (SBR) system

Author

Gloria María Restrepo Vásquez, Juan Miguel Marín Sepúlveda, Ximena María Vargas Ramírez, Kelly Viviana Patiño López, and Gina Maria Hincapié Mejía

Subjects
Castor Oil, Environmental Engineering, Ultraviolet Rays, Iron, Batch reactor, Industrial Waste, Palm Oil, Waste Disposal, Fluid, Bioreactors, Bioreactor, Plant Oils, Water Science and Technology, Biological Oxygen Demand Analysis, Biodiesel, Waste management, Chemistry, Chemical oxygen demand, Hydrogen Peroxide, Photochemical Processes, Aerobiosis, Waste treatment, Wastewater, Biofuels, Biodiesel production, Sewage treatment, Water Pollutants, Chemical
Abstract

A coupled system of the photo-Fenton advanced oxidation technique and an aerobic sequential batch reactor (SBR) was used to treat wastewater from biodiesel production using either palm or castor oil. The photo-Fenton reaction and biological process were evaluated individually and were effective at treating the wastewater; nevertheless, each process required longer degradation times for the wastewater pollutants compared with the coupled system. The proposed coupled photo-Fenton/aerobic SBR system obtained a 90% reduction of the chemical oxygen demand (COD) in half of the time required for the biological system individually.

Published
2012

18. Holocellulase Activity from Schizophyllum commune Grown on Bamboo: A Comparison with Different Substrates

Author

Jorge Valencia, Edivaldo Ximenes Ferreira Filho, Maria Fátima Grossi-de-Sá, Kelly Johana Dussán Medina, Félix Gonçalves de Siqueira, Gloria María Restrepo Franco, Arnubio Valencia Jiménez, and Blair D. Siegfried

Subjects
Bamboo, biology, Bioconversion, Bambusa, Carbohydrates, Schizophyllum commune, General Medicine, Fungus, Schizophyllum, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, Cellulase, Botany, Microscopy, Electron, Scanning, Xylanase, Bioprocess, Pectinase, Bagasse
Abstract

The natural biodiversity that is found in tropical areas offers countless biotechnological opportunities; especially if we take in account that many biomolecules from several microorganisms have supported for many years, different industrial applications in areas such as pharmacology, agro-industry, bioprocess, environmental technology, and bioconversion. In order to find new lignocellulolytic enzymes and evaluate bamboo fibers as substrate, Schizophyllum commune a fungus with broad distribution was isolated and grown during 15 days in liquid culture medium containing 1% lignocellulosic fibers from bamboo, banana stem, and sugarcane bagasse. The enzymatic activity of xylanase, mannanase, polygalacturonase, CMCase, FPase, and avicelase were evaluated. Sugarcane bagasse and banana stem showed to induce higher hollocellulase activity when compared with bamboo as the main carbon source. The physical mechanism that the fungus uses to degrade bamboo was observed not only in fibers naturally infected but also in healthy fibers that were treated and untreated with enzyme solution. SEM analysis showed the structural disruption and invasion of the vascular bundles, parenchyma cells, and parenchymatous tissues as a consequence of the presence of this fungus and the catalytic action of its enzymes into the plant tissue.

Published
2011

19. Study of the Bandgap of Synthesized Titanium Dioxide Nanoparticules Using the Sol-Gel Method and a Hydrothermal Treatment

Author

Sergio Humberto Valencia Hurtado, Gloria María Restrepo Vásquez, and Juan Miguel Marín Sepúlveda

Subjects
Anatase, Materials science, Diffuse reflectance infrared fourier transform, Band gap, Mineralogy, Hydrothermal treatment, Catalysis, Propanol, chemistry.chemical_compound, chemistry, Titanium dioxide, Methyl orange, General Materials Science, Sol-gel method, Sol-gel, Nuclear chemistry
Abstract

In this work optical properties of titanium dioxide nanoparticles were studied. Titanium dioxide nanoparticles were synthesized by the sol-gel method followed by a hydrothermal treatment using tetraisopropyl orthotitanate (TIOT) and 2-propanol. The synthesized samples were characterized by X-ray diffraction (XRD), UV-Vis diffuse reflectance spectroscopy (UV/DRS) and nitrogen adsorption-desorption methods. The bandgap energy was obtained using the Kubelka-Munk reemission function. The catalyst synthesized with a molar ratio of R1 (water/TIOT) = 3.5 and R2 (2-propanol/TIOT) = 15 has a predominately anatase phase. It also has a high photo degradation of methyl orange compared to TiO2 Degussa P-25. A shift band gap energy of 3.27 was observed

Published
2010

20. Modification and Expression of Beta-1,4-Endoglucanase encoding sequences of fungal origin in Escherichia coli BL21.

Author

Natalia Gutierrez Calle, Gloria Maria Restrepo Franco, and Narmer Fernando Galeano Vanegas

Subjects
Cellulose, heterologous expression, Lignocellulolytic enzymes, Biotechnology, TP248.13-248.65
Abstract

Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol production, promoting the bioeconomy. The objective of the present work was to express lignocellulolytic enzymes of eukaryotic origin in Escherichia coli BL21 (DE3). Initially, endoglucanase eukaryotic genes were selected and modified using bioinformatics methods for their production in E. coli BL21 (DE3) and saccharification of pure cellulose substrates. The gene selected for its modification and expression was eglB from the fungus Aspergillus nidulans. Subsequently the enzyme integrity was tested by 3D modeling and molecular docking, as well as the conformation of its active site and its affinity for substrates of interest. Finally, cloning of the modified gene in plasmid pET151 TOPO was made and transformed in the strain E. coli BL21 (DE3) where several lignocellulose degradation tests were carried out using semiquantitative methods for the enzyme activity in carboxymethylcellulose. The presence of the three genes of interest within the plasmid pET151 TOPO and within the transformed cells of E. coli TOP10 and E. coli BL21 (DE3) was verified by colony PCRs performed. The presence of this gen was corroborated by sequencing. Expression of the modified endoglucanase enzyme was achieved in E. coli BL21 (DE3) expression cells, in soluble and functional form, demonstrated by the hydrolysis of the CMC substrate.

Published
2020
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21. Evaluation of cyanide and heavy metals removal in liquid effluents from small mining’s gold benefit, by adsorption with activated carbon and hydrogen peroxide in Segovia, Antioquia

Author

Claudia Catalina Estrada-Montoya, Gloria Maria Restrepo Franco, and Narmer Fernando Galeano Vanegas

Subjects
gold mining, heavy metals removal, cyanide degradation, heavy metals precipitation, environmental pollution, Technology, Mining engineering. Metallurgy, TN1-997
Abstract

The small gold mining generates toxic substances discharges, being an environmental problem. The objective was to evaluate the removal of cyanide and heavy metals, in liquid effluents from the gold benefit, by adsorption with activated carbon and hydrogen peroxide. The residues were first treated with carbon to determine the adsorption efficiency with 20, 40, 60 g of carbon / L of solution at times of 4, 8, 12 hours. Then hydrogen peroxide (1.0, 1.5, 2.0 liters of peroxide / Kg CN in solution, was added over 4 hours). The response variables were concentrations of cyanide, lead, zinc, iron. The best treatment with carbon was 60 g of carbon / L of solution and 12 hours of contact and for the process with hydrogen peroxide: 2 liters of H2O2 / Kg of CN in solution, during 4 hours. A flow chart and tables for the implementation of the process were designed.

Published
2020
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22. Evaluación del suministro de un alimento especial en el transporte y dos primeros días de vida del pollo de engorde y los beneficios sobre su desempeño zootécnico y económico.

Author

Peláez, Javier Darío Chica, Quijano, Gloria María Restrepo, González, Natalia Andrea, Ríos, Beatriz Llano, and Peláez, Andrés Valderrama

Subjects
FOOD supply, FEED utilization efficiency, BODY weight, INCUBATORS, BROILER chickens, ANIMAL nutrition, AGRICULTURAL experiment stations
Abstract

Copyright of CES Medicina Veterinaria & Zootecnica is the property of Universidad CES and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2010

23. EVALUACIÓN DEL TRATAMIENTO FOTOCATALÍTICO DE AGUAS RESIDUALES INDUSTRIALES EMPLEANDO ENERGÍA SOLAR

Author

GLORIA MARIA RESTREPO, LUIS A. RIOS, JUAN MIGUEL MARIN, JUAN FELIPE MONTOYA, and JORGE ARMANDO VELÁSQUEZ

Subjects
Technology, Mining engineering. Metallurgy, TN1-997
Abstract

En este trabajo se evaluó la degradación fotocatalítica de contaminantes acuosos provenientes de una industria de productos químicos, empleando un fotorreactor solar de placas planas de vidrio corrugado dispuestas en forma de cascada, sobre las cuales se soportaron películas de TiO2 (Degussa P-25) / SiO2, como sistema fotoactivo. Se estudió la influencia de tres variables en la eficiencia de descontaminación: cantidad de H2O2, volumen de agua a tratar y cantidad de TiO2 disperso, mediante un diseño de experimentos llevados a cabo en modo de operación discontinua por un periodo de 5 horas para cada ensayo. Los resultados obtenidos confirman a la Fotocatálisis Heterogénea (FH) como una tecnología viable de tratamiento en etapas previas a un tratamiento biológico ya que se alcanzaron porcentajes de reducción en la DQO que varían entre 6 y 46% y se obtuvieron efluentes biodegradables en todas las pruebas realizadas.

Published
2008

24. Bacterias solubilizadoras de fosfato y sus potencialidades de uso en la promoción del crecimiento de cultivos de importancia económica

Author

Gloria María Restrepo Franco, Sandra Marulanda-Moreno, Yeised de la Fe-Pérez, Acela Díaz-de la Osa, Vera Lucia-Baldani, and Annia Hernández-Rodríguez

Subjects
lcsh:Biology (General), lcsh:QR1-502, lcsh:QH301-705.5, lcsh:Microbiology
Abstract

La mayoría de los suelos tropicales y subtropicales son deficientes en fósforo biodisponible, por lo que el empleo de bacterias promotoras del crecimiento vegetal, principalmente las solubilizadoras de fosfato, pueden reducir el uso de fertilizantes químicos. El objetivo de este trabajo es ofrecer una panorámica actual acerca de los principales géneros y mecanismos de acción de las bacterias solubilizadoras de fosfatos (BSF), así como analizar su posible empleo en cultivos de importancia económica. La utilización de géneros bacterianos con mayores potencialidades de uso como Pseudomonas y Bacillus unido a aislados promisorios de Azospirillum y Herbaspirilum en cultivos como arroz y café permitiría reducir a largo plazo el uso de productos químicos en la agricultura, así como desarrollar estrategias agronómicas que preserven el medio ambiente. El éxito de estos inoculantes bacterianos depende de la selección de cepas autóctonas eficientes por tipo de suelo, su capacidad de colonizar la rizosfera y de mantener la actividad biológica.

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