1. Isolation, Purification of Phenolic Glycoside 1 from Moringa oleifera Seeds and Formulation of Its Liposome Delivery System.
- Author
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Shi F, Gong M, Adu-Frimpong M, Jiang X, Wang X, Hua Q, Li T, Li J, Yu J, Toreniyazov E, Cao X, Wang Q, and Xu X
- Subjects
- Humans, Animals, Hep G2 Cells, Particle Size, Drug Delivery Systems methods, Mice, Male, Rats, Administration, Oral, Chemistry, Pharmaceutical methods, Rats, Sprague-Dawley, Liposomes, Moringa oleifera chemistry, Seeds chemistry, Glycosides chemistry, Glycosides administration & dosage, Glycosides pharmacology, Glycosides isolation & purification, Biological Availability, Phenols administration & dosage, Phenols chemistry, Phenols isolation & purification, Phenols pharmacokinetics
- Abstract
In this study, N, N '-bis {4- [(α-L- rhamnosyloxy) benzyl]} thiourea (PG-1), a phenolic glycoside compound was purified from Moringa seed. The PG-1 has attracted extensive attention due to its anti-cancer, antioxidant, anti-inflammatory and hypoglycemic properties. However, some of its physicochemical properties such as oral bioavailability has not been studied. Herein, a highly purified PG-1 was extracted and incorporated in multiple layered liposomes (PG-1-L) to avoid its burst release and enhance oral bioavailability. After appropriate characterization, it was discovered that the obtained PG-1-L was stable, homogeneous and well dispersed with the average particle size being 89.26 ± 0.23 nm. Importantly, the in vitro release and in vivo oral bioavailability of PG-1-L were significantly improved compared with PG-1. In addition, MTT results showed that compared with the free PG-1, PG-1-L displayed obvious inhibitory effect on the HepG2 cells, while the inhibitory effect on healthy non-malignant 3T6 and LO-2 cells was not significant, indicating that PG-1-L had high safety. In conclusion, PG-1-L can be used as a promising delivery system and an ideal novel approach to improve the oral bioavailability and anticancer activity of PG-1., (© 2024. The Author(s), under exclusive licence to American Association of Pharmaceutical Scientists.)
- Published
- 2024
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