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2 results on '"GmCCD4 gene"'

1. GmCCD4 controls carotenoid content in soybeans.

Author

Gao, Jinshan, Yang, Suxin, Tang, Kuanqiang, Li, Guang, Gao, Xiang, Liu, Bao, Wang, Shaodong, and Feng, Xianzhong

Subjects
*LOCUS (Genetics), *FLOWER petals, *GENES, *PLANT metabolism, *HAPLOTYPES, *CAROTENOIDS, *SEEDS
Abstract

Summary: To better understand the mechanisms regulating plant carotenoid metabolism in staple crop, we report the map‐based cloning and functional characterization of the Glycine max carotenoid cleavage dioxygenase 4 (GmCCD4) gene, which encodes a carotenoid cleavage dioxygenase enzyme involved in metabolizing carotenoids into volatile β‐ionone. Loss of GmCCD4 protein function in four Glycine max increased carotenoid content (gmicc) mutants resulted in yellow flowers due to excessive accumulation of carotenoids in flower petals. The carotenoid contents also increase three times in gmicc1 seeds. A genome‐wide association study indicated that the GmCCD4 locus was one major locus associated with carotenoid content in natural population. Further analysis indicated that the haplotype‐1 of GmCCD4 gene was positively associated with higher carotenoid levels in soybean cultivars and accumulated more β‐carotene in engineered E. coli with ectopic expression of different GmCCD4 haplotypes. These observations uncovered that GmCCD4 was a negative regulator of carotenoid content in soybean, and its various haplotypes provide useful resources for future soybean breeding practice. [ABSTRACT FROM AUTHOR]

Published
2021
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2. GmCCD4 controls carotenoid content in soybeans

Author

Bao Liu, Suxin Yang, Xiang Gao, Guang Li, Kuanqiang Tang, Xianzhong Feng, Gao Jinshan, and Shaodong Wang

Subjects
0106 biological sciences, 0301 basic medicine, Mutant, Locus (genetics), Plant Science, macromolecular substances, Biology, 01 natural sciences, Dioxygenases, 03 medical and health sciences, Gene mapping, yellow flower, Dioxygenase, Gene Expression Regulation, Plant, polycyclic compounds, Escherichia coli, Food science, soybean, Gene, Carotenoid, Research Articles, Plant Proteins, chemistry.chemical_classification, GmCCD4 gene, organic chemicals, carotenoids, food and beverages, biological factors, Plant Breeding, 030104 developmental biology, chemistry, Petal, Ectopic expression, genetic mapping, Soybeans, Agronomy and Crop Science, 010606 plant biology & botany, Biotechnology, Research Article, Genome-Wide Association Study
Abstract

Summary To better understand the mechanisms regulating plant carotenoid metabolism in staple crop, we report the map‐based cloning and functional characterization of the Glycine max carotenoid cleavage dioxygenase 4 (GmCCD4) gene, which encodes a carotenoid cleavage dioxygenase enzyme involved in metabolizing carotenoids into volatile β‐ionone. Loss of GmCCD4 protein function in four Glycine max increased carotenoid content (gmicc) mutants resulted in yellow flowers due to excessive accumulation of carotenoids in flower petals. The carotenoid contents also increase three times in gmicc1 seeds. A genome‐wide association study indicated that the GmCCD4 locus was one major locus associated with carotenoid content in natural population. Further analysis indicated that the haplotype‐1 of GmCCD4 gene was positively associated with higher carotenoid levels in soybean cultivars and accumulated more β‐carotene in engineered E. coli with ectopic expression of different GmCCD4 haplotypes. These observations uncovered that GmCCD4 was a negative regulator of carotenoid content in soybean, and its various haplotypes provide useful resources for future soybean breeding practice.

Published
2020

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