Search

Bolotov, Ivan N., Eliseeva, Tatyana A., Kondakov, Alexander V., Gofarov, Mikhail Y., Aksenova, Olga V., Bespalaya, Yulia V., Kropotin, Alexander V., Travina, Oksana V., Vinarski, Maxim V. (2023): Barbronia borealis sp. nov., the first salifid leech discovered in Russia, with a global checklist of this genus. Ecologica Montenegrina 63: 24-38, DOI: 10.37828/em.2023.63.3, URL: http://dx.doi.org/10.37828/em.2023.63.3

Barbronia borealis Bolotov, Eliseeva & Kondakov sp. nov. https://zoobank.org/ urn:lsid:zoobank.org:act: 06EF7905-A09C-4F37-B686-36A0D67A7F7D Figures 4, 5 a-c Holotype: RMBH Hir_0405 (fixed and stored in 96% ethanol); RUSSIA: Kiparisovka River, 43.4578°N, 131.9017°E, Razdolnaya (Suifun) River basin, Primorye Region, September 05, 2020, O. V. Aksenova, Y. V. Bespalaya, A. V. Kropotin, O. V. Travina & M. V. Vinarski leg. Etymology: The name of this species reflects its record in the boreal zone of Eurasia. Differential diagnosis: The new species is externally similar to Barbronia gwalagwalensis and B. weberi. It differs from B. gwalagwalensis by having 5.5 annuli between gonopores (vs 7.5 annuli) and from B. weberi by having 6 annuli between accessory pore and corresponding gonopore (vs 5 annuli). However, DNA barcoding should be considered the most reliable approach for identification of these morphologically similar species. DNA-based diagnosis: The reference DNA sequences of the holotype: OQ940656 (COI) and OQ941865 (18S rRNA). This species represents a divergent phylogenetic lineage, which is distant from other species in the genus, the sequences of which are available (Table 2). Description: Small salifid leech: body length 20.1 mm, maximum body width 3.3 mm, maximum width of anterior sucker 0.9 mm, maximum width of posterior sucker 2.0 mm (Figure 4). Body elongated, vermiform, tapering anteriorly. Body surface smooth, without papillae. Posterior sucker ventrally directed. Dorsum light ochraceous, venter whitish. Anterior region with three pairs of circular eyespots: one labial on 3rd annulus and two buccal on 6-7th annuli (Figure 5 a-b). Complete mid-body somite 6 annulate: b1 + b2 + a2 + b5 + c11 + c12 (Figure 5c). Clitellum extends from X b5 to XIV b2. Gonopores large, well visible, separated by 5.5 annuli. Male accessory pore in the furrow X c12/XI b1, male gonopore in XII b1/b2, female gonopore in XIII b1, female accessory pore in XIII c12/XIV b1 (Figure 5c). Anus dorsally at XXVII, two postanal annuli anterior to posterior sucker (Figure 4). Reproductive and digestive systems remain unstudied because only the holotype was available for description. Distribution: This species is only known from its type locality, situated at the southeastern corner of the Russian Far East. However, numerous occurrences from Northeastern China attributed to B. cf. weberi (Figure 1) may belong to the new species. Habitats and ecology: The holotype was collected from a pool site of a small river with clay bottom (Figure 6). This leech specimen was fixed with its prey, an oligochaete, protruding from its mouth. A COI sequence was generated from this worm (GenBank acc. No. OQ933549). Searching with the BOLD IDS reveals that the COI sequence of the prey item is related to those of oligochaetes identified as Limnodrilus profundicola (Verrill, 1871) (Oligochaeta: Naididae). This finding indicates that Barbronia borealis sp. nov. feeds on small freshwater oligochaetes, as do some other species in this genus (Nesemann et al. 2007)., Published as part of Bolotov, Ivan N., Eliseeva, Tatyana A., Kondakov, Alexander V., Gofarov, Mikhail Y., Aksenova, Olga V., Bespalaya, Yulia V., Kropotin, Alexander V., Travina, Oksana V. & Vinarski, Maxim V., 2023, Barbronia borealis sp. nov., the first salifid leech discovered in Russia, with a global checklist of this genus, pp. 24-38 in Ecologica Montenegrina 63 on pages 28-30, DOI: 10.37828/em.2023.63.3, http://zenodo.org/record/8082901, {"references":["Nesemann, H., Sharma, S., Sharma, G., Khanal, S. N., Pradhan, B., Shah, D. N. & Tachamo, R. D. (2007) Aquatic Invertebrates of the Ganga River System: Volume 1 - Mollusca, Annelida, Crustacea (in part). Hasko Nesemann and Chandi Press, Kathmandu, 263 pp."]}

Nagel, Karl-Otto, Schiøtte, Tom, Gofarov, Mikhail Y., Vikhrev, Ilya V., Konopleva, Ekaterina S., Bolotov, Ivan N. (2022): Re-discovery of the type series of the Indian freshwater mussel Parreysia corrugata (O. F. Müller, 1774) with the designation of the lectotype (Bivalvia: Unionidae: Parreysiinae). Journal of Natural History (J. Nat. Hist.) 56 (5-8): 493-511, DOI: 10.1080/00222933.2022.2063083, URL: http://dx.doi.org/10.1080/00222933.2022.2063083

Barbronia gwalagwalensis Westergren & Siddall, 2004 Figures 3-6 Type. Holotype, AMNH Annelida 5261, fixed in 10% buffered formalin and stored in 70% ethanol (American Museum of Natural History, New York, USA). Type locality. ��� Maia���s Dam, Gwalagwala, a tented-camp near Hoedspruit, South Africa ��� (Westergren & Siddall 2004). Material examined. MYANMAR: Salween River drainage, Lake Inle basin, Kyee Phyu Lake, 20.8142��N, 96.9690��E, 23.ii.2018, 2 specimens, fixed in 96% ethanol [voucher RMBH Hir_58_3; one specimen sequenced: COI acc. no. MN 295405]. The sample is deposited in the RMBH ��� Russian Museum of Biodiversity Hotspots, N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences, Arkhangelsk, Russia. Morphology of Myanmar���s sample. Small leeches, 10���15 mm long (ethanol-preserved specimens). Body subcylindrical, vermiform, tapering anteriorly and posteriorly. Dorsum light brown. Three pairs of eyespots: first dorsal, on II; second and third pairs dorsolateral on IV, and separated from anterior pair by four complete annuli. Caudal sucker directed ventrally. Clitellum indistinct (juveniles), with male and female gonopores separated by seven and one-half annuli, and with two accessory copulatory pores ventrally: one anterior of the male gonopore at X/XI, and second posterior of the female gonopore at XIII/XIV. Three pairs of pharyngeal stylets. These features correspond well to the protologue of the species, although the type series comprised full-grown adults up to 25 mm long with pronounced clitellum and distinct accessory copulatory pores (Westergren & Siddall 2004). Habitat. The specimens were collected on a submerged bamboo stick and an empty shell of the freshwater mussel species Lamellidens ferrugineus (Annandale) found near the shore of a shallow eutrophic lake with silty bottom and rich aquatic plant assemblages. They co-occurred with the freshwater glossiphonid species Alboglossiphonia sp. (Hirudinida: Glossiphoniidae) [voucher no. RMBH 58_2; COI acc. no. MN 295404] and the triclad species Dugesia sp. (Tricladida: Dugesiidae) [voucher no. RMBH 58_1]. The Dugesia species was very abundant. Distribution. This species appears to be widespread throughout the Paleotropical Region as it was found in South Africa, Southeast Asia (Myanmar), and East Asia (Korea). The occurrence from the Durance River (Rh��ne Basin) in southeastern France (Corse et al. 2017) most likely reflects a recent human-mediated or native dispersal event towards southern Europe. Comments. The nominal taxon Trocheta quadrioculata Oka, 1922 was described from the Inle Lake in Myanmar (Oka 1922). The type locality of this species is close to our finding of B. gwalagwalensis (ca. 25-30 km SSW). Currently, Inle Lake���s taxon is placed within Salifidae as Odontobdella quadrioculata (see Nesemann & Sharma 2012). Based on the protologue (Oka 1922), it does not have accessory copulatory pores and, hence, cannot be linked to the genus Barbronia (see Westergren & Siddall 2004). The fauna of the family Salifidae in Myanmar seems to be largely underestimated, and comprises only two species, i.e. B. gwalagwalensis and O. quadrioculata., Published as part of Klass, Anna L., Kondakov, Alexander V., Vikhrev, Ilya V., Bespalaya, Yulia V., Lunn, Zau, Chan, Nyein, Gofarov, Mikhail Y. & Bolotov, Ivan N., 2021, Is the South African leech Barbronia gwalagwalensis Westergren & Siddall, 2004 (Hirudinida: Erpobdelliformes: Salifidae) a Paleotropical species?, pp. 585-595 in Zootaxa 4974 (3) on pages 589-591, DOI: 10.11646/zootaxa.4974.3.7, http://zenodo.org/record/4778124, {"references":["Westergren, S. & Siddall, M. E. (2004) Two new species of salifid leeches (Arhynchobdellida: Erpobdelliformes: Salifidae) from South Africa and Madagascar. American Museum Novitates, 3456, 1 - 6. https: // doi. org / 10.1206 / 0003 - 0082 (2004) 456 2.0. CO; 2","Corse, E., Meglecz, E., Archambaud, G., Ardisson, M., Martin, J. F., Tougard, C., Chappaz, R. & Dubut, V. (2017) A from benchtop to desktop workflow for validating HTS data and for taxonomic identification in diet metabarcoding studies. Molecular Ecology Resources, 17 (6), e 146 - e 159. https: // doi. org / 10.1111 / 1755 - 0998.12703","Oka, A. (1922) Hirudinea from Inle Lake, S. Shan States, Burma. Records of the Indian Museum, 24, 521 - 534.","Nesemann, H. & Sharma, G. (2012) Description of a new species of the leech family Salifidae (Odontobdella krishna sp. nov.) from the River Ganga at Patna, Bihar (India). Records of the Zoological Survey of India, 111 (3), 1 - 7."]}

Klass, Anna L., Kondakov, Alexander V., Vikhrev, Ilya V., Bespalaya, Yulia V., Lunn, Zau, Chan, Nyein, Gofarov, Mikhail Y., Bolotov, Ivan N. (2021): Is the South African leech Barbronia gwalagwalensis Westergren & Siddall, 2004 (Hirudinida: Erpobdelliformes: Salifidae) a Paleotropical species? Zootaxa 4974 (3): 585-595, DOI: https://doi.org/10.11646/zootaxa.4974.3.7

Bolotov, Ivan N., Vikhrev, Ilya V., Lopes-Lima, Manuel, Gofarov, Mikhail Y., Konopleva, Ekaterina S., Lyubas, Artem A., Lunn, Zau, Chan, Nyein, Bogan, Arthur E. (2019): Unio sella and U. sula: A review of enigmatic taxonomic names linked to Gibbosula laosensis (Lea, 1863) (Bivalvia: Margaritiferidae: Gibbosulinae). Raffles Bulletin of Zoology 67: 440-447, DOI: 10.26107/RBZ-2019-0035

Unio sula Simpson, 1900 Unio sula Simpson, 1900: 678; Haas, 1912: 122; Simpson, 1914: 521; Brandt, 1974: 260. Taxonomic status. Nomen nudum, probably an incorrect spelling of the name Unio sella Prashad, 1922 (see Fig. 4D and Discussion)., Published as part of Bolotov, Ivan N., Vikhrev, Ilya V., Lopes-Lima, Manuel, Gofarov, Mikhail Y., Konopleva, Ekaterina S., Lyubas, Artem A., Lunn, Zau, Chan, Nyein & Bogan, Arthur E., 2019, Unio sella and U. sula: A review of enigmatic taxonomic names linked to Gibbosula laosensis (Lea, 1863) (Bivalvia: Margaritiferidae: Gibbosulinae), pp. 440-447 in Raffles Bulletin of Zoology 67 on page 446, DOI: 10.26107/RBZ-2019-0035, http://zenodo.org/record/4577110, {"references":["Simpson CT (1900) Synopsis of the naiades, or pearly fresh-water mussels. Proceedings of the United States National Museum, 22: 501 - 1044.","Haas F (1912) Die Unioniden. In: Kuster HC (ed.) Systematisches Conchylien-Cabinet von Martini und Chemnitz (Bd. 9, Abt. 2, H. 46). Pp. 113 - 136, pls. 30 - 35.","Simpson CT (1914) A descriptive catalogue of the naiades, or pearly fresh-water mussels. Parts I-III. Bryant Walker, Detroit, 1540 pp.","Brandt RAM (1974) The non-marine aquatic Mollusca of Thailand. Archiv fur Molluskenkunde, 105: 1 - 423.","Prashad B (1922) A revision of the Burmese Unionidae. Records of the Indian Museum, 24: 91 - 111."]}