1. A novel and ultrasensitive sandwich-type electrochemical immunosensor based on delaminated MXene@AuNPs as signal amplification for prostate specific antigen (PSA) detection and immunosensor validation
- Author
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Hilal Medetalibeyoglu, Gül Kotan, Mehmet Lütfi Yola, Necip Atar, Mühendislik ve Doğa Bilimleri Fakültesi -- Biyomedikal Mühendisliği Bölümü, and Yola, Mehmet Lütfi
- Subjects
Male ,Immunosensors ,Cyclic voltammetry ,Scanning electron microscope ,X ray photoelectron spectroscopy ,Nanoparticle ,High resolution transmission electron microscopy ,02 engineering and technology ,Biosensing Techniques ,Immunosensor ,01 natural sciences ,Analytical Chemistry ,Limit of Detection ,d-Ti3C2TX MXene@AuNPs ,Validation ,Composites ,Immunoassay ,Chemistry ,Fourier transform infrared spectroscopy ,021001 nanoscience & nanotechnology ,Dielectric spectroscopy ,Colloidal gold ,Electrochemical immunosensors ,Linearity range ,Graphite ,Glass membrane electrodes ,Detection limits ,0210 nano-technology ,Prostate specific antigen ,Scanning electron microscopy ,Electrochemical impedance spectroscopy ,Metal nanoparticles ,Titanium Carbide | Sodium-ion Batteries | Ion Storage ,Catalysis ,X-ray photoelectron spectroscopy ,Fiber optic sensors ,Humans ,Gold nanoparticles ,Antigens ,Sensor ,Detection limit ,010401 analytical chemistry ,Functionalized graphene ,Electrochemical Techniques ,Prostate-Specific Antigen ,Analytical ,Carbon ,0104 chemical sciences ,Glassy carbon electrodes ,Gold nanoparticles/graphene oxide composite ,Nanoparticles ,Gold ,Ultra sensitives ,Graphene ,Signal amplifications ,Prostate-specific antigen (PSA) ,Nuclear chemistry - Abstract
In this study, an ultra-sensitive electrochemical immunosensor for PSA detection was designed. Firstly, gold nanoparticles (AuNPs)/p-aminothiophenol (ATP) functionalized graphene oxide (GO) composite (AuNPs-ATPGO) was constructed and modified to glassy carbon electrode (AuNPs-ATPGO/GCE). AuNPs-ATPGO/GCE was utilized as immunosensor platform to increase the amount of PSA antibody1 (Ab1). After that, self-assembled delaminated MXene-gold nanoparticles (d-Ti3C2TX MXene@AuNPs) was used to label PSA seconder antibody2 (Ab2) as signal amplification. The prepared AuNPs-ATPGO, d-Ti3C2TX MXene@AuNPs and electrochemical immunosensor was charecterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscope (SEM), x-ray diffraction (XRD) method, x-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR). The linearity range and detection limit (LOD) were obtained as 0.01–1.0 pg mL-1 and 3.0 fg mL-1, respectively. © 2020 Elsevier B.V.
- Published
- 2020