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31 results on '"Goldberg, Jacob M."'

5. Rapid Electrophilic Cysteine Arylation with Pyridinium Salts

Author

Lipka, Bradley M., primary, Betti, Vincent M., additional, Honeycutt, Daniel S., additional, Zelmanovich, Daniel L., additional, Adamczyk, Max, additional, Wu, Ruojun, additional, Blume, Harrison S., additional, Mendina, Caitlin A., additional, Goldberg, Jacob M., additional, and Wang, Fang, additional

Published
2022
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6. Mobile zinc as a modulator of sensory perception.

Author

Goldberg, Jacob M. and Lippard, Stephen J.

Subjects
*SENSORY perception, *SENSE organs, *ZINC, *TRANSITION metal ions, *CHELATING agents
Abstract

Mobile zinc is an abundant transition metal ion in the central nervous system, with pools of divalent zinc accumulating in regions of the brain engaged in sensory perception and memory formation. Here, we present essential tools that we developed to interrogate the role(s) of mobile zinc in these processes. Most important are (a) fluorescent sensors that report the presence of mobile zinc and (b) fast, Zn‐selective chelating agents for measuring zinc flux in animal tissue and live animals. The results of our studies, conducted in collaboration with neuroscientist experts, are presented for sensory organs involved in hearing, smell, vision, and learning and memory. A general principle emerging from these studies is that the function of mobile zinc in all cases appears to be downregulation of the amplitude of the response following overstimulation of the respective sensory organs. Possible consequences affecting human behavior are presented for future investigations in collaboration with interested behavioral scientists. [ABSTRACT FROM AUTHOR]

Published
2023
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10. Native Zinc Catalyzes Selective and Traceless Release of Small Molecules in β-Cells

Author

Lee, Miseon, primary, Maji, Basudeb, additional, Manna, Debasish, additional, Kahraman, Sevim, additional, Elgamal, Ruth M., additional, Small, Jonnell, additional, Kokkonda, Praveen, additional, Vetere, Amedeo, additional, Goldberg, Jacob M., additional, Lippard, Stephen J., additional, Kulkarni, Rohit N., additional, Wagner, Bridget K., additional, and Choudhary, Amit, additional

Published
2020
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13. Photoactivatable Sensors for Detecting Mobile Zinc

Author

Goldberg, Jacob M., primary, Wang, Fang, additional, Sessler, Chanan D., additional, Vogler, Nathan W., additional, Zhang, Daniel Y., additional, Loucks, William H., additional, Tzounopoulos, Thanos, additional, and Lippard, Stephen J., additional

Published
2018
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14. Thioamides as fluorescence quenching probes: minimalist chromophores to monitor protein dynamics

Author

Goldberg, Jacob M., Batkargal, Solongo, and Petersson, E. James

Subjects
Chromophores -- Structure, Chromophores -- Chemical properties, Chromophores -- Optical properties, Fluorescence -- Analysis, Phenylalanine -- Structure, Phenylalanine -- Chemical properties, Substitution reactions -- Analysis, Proteins -- Structure, Proteins -- Analysis, Chemistry
Abstract

p-Cyanophenylalanine (Cnf) and backbone thioamides are developed as a fluorophore/quencher pair. The studies have shown that for a given protein, thioamide substitutions is used to track motions with a much greater number of measurements than for current fluorescence probes, which has provided a dense array of data with which to model conformational changes.

Published
2010

15. CF2H, a Hydrogen Bond Donor

Author

Sessler, Chanan D., primary, Rahm, Martin, additional, Becker, Sabine, additional, Goldberg, Jacob M., additional, Wang, Fang, additional, and Lippard, Stephen J., additional

Published
2017
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17. Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and FRET/LRET Studies

Author

Speight, Lee C., Muthusamy, Anand K., Goldberg, Jacob M., Warner, John B., Wissner, Rebecca F., Willi, Taylor S., Woodman, Bradley F., Mehl, Ryan A., and Petersson, E. James

Subjects
Models, Molecular, Alanine, Luminescence, Fluorescence Resonance Energy Transfer, Acridines, Article
Abstract

The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation of Acd into proteins expressed in Escherichia coli requires efficient chemical synthesis to produce large quantities of the amino acid and the generation of a mutant aminoacyl tRNA synthetase that can selectively charge the amino acid onto a tRNA. Here, we report the synthesis of Acd in 87% yield over five steps from Tyr and the identification of an Acd synthetase by screening candidate enzymes previously evolved from Methanococcus janaschii Tyr synthetase for unnatural amino acid incorporation. Furthermore, we characterize the photophysical properties of Acd, including quenching interactions with select natural amino acids and Förster resonance energy transfer (FRET) interactions with common fluorophores such as methoxycoumarin (Mcm). Finally, we demonstrate the value of incorporation of Acd into proteins, using changes in Acd fluorescence lifetimes, Mcm/Acd FRET, or energy transfer to Eu(3+) to monitor protein folding and binding interactions.

Published
2013

22. Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and Förster Resonance Energy Transfer/Luminescence Resonance Energy Transfer Studies

Author

Speight, Lee C., primary, Muthusamy, Anand K., additional, Goldberg, Jacob M., additional, Warner, John B., additional, Wissner, Rebecca F., additional, Willi, Taylor S., additional, Woodman, Bradley F., additional, Mehl, Ryan A., additional, and Petersson, E. James, additional

Published
2013
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24. Analysis of Maternal Risk Factors Associated With Congenital Vertebral Malformations

Author

Hesemann, Jennifer, primary, Lauer, Emily, additional, Ziska, Stephen, additional, Noonan, Kenneth, additional, Nemeth, Blaise, additional, Scott-Schwoerer, Jessica, additional, McCarty, Catherine, additional, Rasmussen, Kristen, additional, Goldberg, Jacob M., additional, Sund, Sarah, additional, Eickhoff, Jens, additional, Raggio, Cathleen L., additional, and Giampietro, Philip F., additional

Published
2013
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31. CF 2 H, a Hydrogen Bond Donor.

Author

Sessler CD, Rahm M, Becker S, Goldberg JM, Wang F, and Lippard SJ

Subjects
Hydrogen Bonding, Nitrophenols chemistry, Quantum Theory, Spectrophotometry, Infrared, Toluene analogs & derivatives, Toluene chemistry, Hydrocarbons, Fluorinated chemistry, Hydroxyl Radical chemistry
Abstract

The CF 2 H group, a potential surrogate for the OH group, can act as an unusual hydrogen bond donor, as confirmed by crystallographic, spectroscopic, and computational methods. Here, we demonstrate the bioisosterism of the OH and CF 2 H groups and the important roles of CF 2 -H···O hydrogen bonds in influencing intermolecular interactions and conformational preferences. Experimental evidence, corroborated by theory, reveals the distinctive nature of CF 2 H hydrogen bonding interactions relative to their normal OH hydrogen bonding counterparts.

Published
2017
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