Search

Increases in physical (e.g., high-intensity running and sprinting), technical (e.g., passing rate), and tactical (e.g., player density) aspects made elite level soccer more challenging within the past years. The aim of the study was to evaluate whether these evolutions are also been reflected in changes in anthropometric and fitness characteristics between former (2002 to 2005) and current (2012 to 2015) elite Austrian youth development center (U13 to U14) and soccer academy (U15 to U18) players. A battery of anthropometric, general and soccer-specific fitness tests was conducted annually at the end of each year. Independent t-test and Cohens d (ES) were calculated to compare the two four-year periods (2530 vs. 2611 players) at each age group separately. Current players were significantly faster in 20 m sprint (ES = 0.260.50) and reaction test (ES = 0.150.39, except for U18), but less flexible at sit-and-reach (ES = 0.19 to 0.55), in all age categories. Whereas height (ES = 0.260.32), body mass (ES = 0.110.18) and countermovement jump (ES = 0.240.26) increased significantly at youth development center level, current academy players performed superior at shuttle sprint (ES = 0.210.59), hurdles agility run (ES = 0.240.49), and endurance run (ES = 0.110.20). These changes over time in speed, change-of-direction ability, lower-body power, coordination, and endurance were attributed to modern training approaches (e.g., modified games and change-of-direction drills) and modifications in selection politics (e.g., coaches favor speed and decision-making skills). (VLID)3853532

Agaricus bisporus is a litter degrading basidiomycete commonly found in humic-rich environments. It is used as model organism and cultivated in large scale for food industry. Due to its ecological niche it produces a variety of enzymes for detoxification and degradation of humified plant litter. One of these, pyranose dehydrogenase, is thought to play a role in detoxification and lignocellulose degradation. It is a member of the glucose-methanol-choline family of flavin-dependent enzymes and oxidizes a wide range of sugars with concomitant reduction of electron acceptors like quinones.In this work, transcription of pdh in A. bisporus was investigated with real-time PCR revealing influence of the carbon source on pdh expression levels. The gene was isolated and heterologously expressed in Pichia pastoris. Characterization of the recombinant enzyme showed a higher affinity towards disaccharides compared to other tested pyranose dehydrogenases from related Agariceae. Homology modeling and sequence alignments indicated that two loops of high sequence variability at substrate access site could play an important role in modulating these substrate specificities.

The aim of the study was to assess the role of the relative age effect (RAE) and to investigate the influence of the biological maturity status on the RAE in international under-9 soccer. The birth dates of 222 male participants of the U9 Eurochampionship Soccer Tournament in Vienna in 2016 were analyzed and divided into four relative age quarters (Q1-Q4) and the biological maturity status was assessed with the age at peak height velocity (APHV) method. Based on the meanstandard deviation of the APHV, the athletes were divided into three groups of maturity: early, normal and late maturing. Chi-Square-tests were used to assess the difference between the observed and the expected even relative age quarter distribution and to evaluate the difference between the observed distribution of early, normal and late maturing athletes and the expected normal distribution. A univariate analysis of variance was performed to assess differences in the APHV between the relative age quarters. A RAE was present (2 = 23.87; p < 0.001; = 0.33). A significant difference was found in APHV between the four relative age quarters (F = 9.906; p < 0.001); relatively older athletes were significantly less mature. A significant difference was found between the distribution of early, normal and late maturing athletes and the expected normal distribution for athletes of Q1 (high percentage of late maturing athletes: 27%; 2 = 17.69; p < 0.001; = 0.46) and of Q4 (high percentage of early maturing soccer players: 31%; 2 = 12.08; p = 0.002; = 0.58). These findings demonstrated that the selection process in international soccer, with athletes younger than 9 years, seems to be associated with the biological maturity status and the relative age. Relatively younger soccer players seem to have a better chance for selection for international tournaments, if they enter puberty at an earlier age, whereas relatively older athletes seem to have an increased likelihood for selection independent of their biological maturity status. (VLID)2639794

Christoph Gonaus Zusammenfassung in deutscher Sprache Universität für Bodenkultur Wien, Dissertation, 2016 OeBB

The present study focuses on fragmented deglycosylated pyranose dehydrogenase (fdgPDH) from Agaricus meleagris recombinantly expressed in Pichia pastoris. Fragmented deglycosylated PDH is formed from the deglycosylated enzyme (dgPDH) when it spontaneously loses a C-terminal fragment when stored in a buffer solution at 4 degrees C. The remaining larger fragment has a molecular weight of similar to 46 kDa and exhibits higher volumetric activity for glucose oxidation compared with the deglycosylated and glycosylated (gPDH) forms of PDH. Flow injection amperometry and cyclic voltammetry were used to assess and compare the catalytic activity of the three investigated forms of PDH, "wired" to graphite electrodes with two different osmium redox polymers: [Os(4,4'-dimethyl-2,2'-bipyridine)(2)(poly(vinylimidazole))(10)Cl](+) [Os(dmbpy)PVI] and [Os(4,4'-dimethoxy-2,2'-bipyridine)(2)(poly-(vinylimidazole))(10)Cl](+) [Os(dmobpy)PVI]. When "wired" with Os(dmbpy)PVI, the graphite electrodes modified with fdgPDH showed a pronounced increase in the current density with J(max). 13- and 6-fold higher than that observed for gPDH- and dgPDH-modified electrodes, making the fragmented enzyme extraordinarily attractive for further biotechnological applications. An easier access of the substrate to the active site and improved communication between the enzyme and mediator matrix are suggested as the two main reasons for the excellent performance of the fdgPDH when compared with that of gPDH and dgPDH. Three of the four glycosites in PDH: N-75, N-175, and N-252 were assigned using mass spectrometry in conjunction with endoglycosidase treatment and tryptic digestion. Determination of the asparagine residues carrying carbohydrate moieties in PDH can serve as a solid background for production of recombinant enzyme lacking glycosylation.

eingereicht von Christoph Gonaus Salzburg, Univ., Dipl.-Arb., 2010 Abstract in dt. Sprache