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2 results on '"Gondo Higashi H"'

1. A new anti-loxoscelic serum produced against recombinant sphingomyelinase D: results of preclinical trials.

Author

de Almeida DM, Fernandes-Pedrosa Mde F, de Andrade RM, Marcelino JR, Gondo-Higashi H, de Azevedo Ide L, Ho PL, van den Berg C, and Tambourgi DV

Subjects
Animals, Cells, Cultured, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Erythrocytes drug effects, Horses, Humans, Immunochemistry, Neutralization Tests, Phosphoric Diester Hydrolases metabolism, Rabbits, Recombinant Proteins antagonists & inhibitors, Recombinant Proteins immunology, Recombinant Proteins metabolism, Spider Venoms enzymology, Spider Venoms immunology, Spiders enzymology, Spiders metabolism, Antivenins pharmacology, Phosphoric Diester Hydrolases immunology, Spider Bites therapy, Spider Venoms antagonists & inhibitors
Abstract

Envenomation by Loxosceles species (brown spider) can lead to local dermonecrosis and to serious systemic effects. The main toxic component in the venom of these spiders is sphingomyelinase D (SMase D) and various isoforms of this toxin are present in Loxosceles venoms. We have produced a new anti-loxoscelic serum by immunizing horses with recombinant SMase D. In the present study, we compared the neutralization efficacy of the new anti-loxoscelic serum and anti-arachnidic serum (the latter serum is used for therapy for loxoscelism in Brazil) against the toxic effects of venoms from spiders of the genus Loxosceles. Neutralization tests showed that anti-SMase D serum has a higher activity against toxic effects of L. intermedia and L. laeta venoms and similar or slightly weaker activity against toxic effects of L. gaucho than that of Arachnidic serum. These results demonstrate that recombinant SMase D can replace venom for anti-venom production and therapy.

Published
2008

2. Role of IgG(T) and IgGa isotypes obtained from arachnidic antivenom to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms.

Author

Toro AF, Malta MB, Soares SL, Da Rocha GC, da Silva Lira M, De Oliveira TA, Takehara HA, Lopes-Ferreira M, Santoro ML, Guidolin R, Gondo Higashi H, Fernandes I, and Barbaro KC

Subjects
Animals, Antivenins chemistry, Chromatography, Liquid, Horses immunology, Immunoglobulin G isolation & purification, Mice, Phosphoric Diester Hydrolases immunology, Phosphoric Diester Hydrolases toxicity, Scorpion Venoms immunology, Scorpion Venoms toxicity, Spider Venoms immunology, Spider Venoms toxicity, Spiders metabolism, Antivenins pharmacology, Immunoglobulin G pharmacology, Scorpion Venoms antagonists & inhibitors, Spider Venoms antagonists & inhibitors, Spiders chemistry
Abstract

The ability of IgG(T) and IgGa subclasses--isolated by liquid chromatography from equine arachnidic antivenom (AAV)-to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms as well as to remove venom toxins from circulation was investigated. These subclasses showed similar antibody titers against L. gaucho, P. nigriventer and T. serrulatus venoms, and by immunoblotting few differences were observed in the recognition pattern of venom antigens. IgG(T) and IgGa neutralized 100% lethality induced by L. gaucho and 50% of P. nigriventer venom, but IgGa failed to neutralize T. serrulatus venom, in contrast to IgG(T). Both subclasses neutralized local reactions and dermonecrosis induced by L. gaucho venom in rabbits. In mice, IgG(T) and IgGa partially neutralized the edematogenic activity induced by P. nigriventer and T. serrulatus venoms, but only IgG(T) neutralized (ca. 81%) the nociceptive activity induced by T. serrulatus venom. Both subclasses failed to neutralize nociceptive activity induced by P. nigriventer venom. IgG(T) reduced the serum venom levels of animals injected with L. gaucho, P. nigriventer or T. serrulatus venoms, while IgGa solely reduced L. gaucho and P. nigriventer venoms levels. Our results demostrate that IgG(T) and IgGa subclasses neutralize toxic activities induced by P. nigriventer, T. serrulatus and L. gaucho venoms with different efficacies, as well as depurate these venoms from circulation.

Published
2006
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