Search

Your search keyword '"Gong, Chengliang"' showing total 401 results
Start Over Author "Gong, Chengliang"
401 results on '"Gong, Chengliang"'

7. Insect Metagenomics

Author

Kundapur, Rajesh, Kumar, Dhiraj, Singh, Shubham, Shukla, Sangeeta, Gong, Chengliang, Bosserhoff, Anja K., Series Editor, Berenjian, Aydin, Series Editor, Carbonell, Pablo, Series Editor, Levite, Mia, Series Editor, Roig, Joan, Series Editor, Turksen, Kursad, Series Editor, Kumar, Dhiraj, editor, and Shukla, Sangeeta, editor

Published
2023
Full Text
View/download PDF

33. A novel chimeric RNA originating from BmCPV S4 and Bombyx mori HDAC11 transcripts regulates virus proliferation.

Author

Pan, Jun, Wei, Shulin, Qiu, Qunnan, Tong, Xinyu, Shen, Zeen, Zhu, Min, Hu, Xiaolong, and Gong, Chengliang

Subjects
SILKWORMS, RNA replicase, RNA virus infections, RNA viruses, DOUBLE-stranded RNA, RNA, CHIMERIC proteins, REVERSE transcriptase
Abstract

Polymerases encoded by segmented negative-strand RNA viruses cleave 5'-m7G-capped host transcripts to prime viral mRNA synthesis ("cap-snatching") to generate chimeric RNA, and trans-splicing occurs between viral and cellular transcripts. Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), an RNA virus belonging to Reoviridae, is a major pathogen of silkworm (B. mori). The genome of BmCPV consists of 10 segmented double-stranded RNAs (S1-S10) from which viral RNAs encoding a protein are transcribed. In this study, chimeric silkworm-BmCPV RNAs, in which the sequence derived from the silkworm transcript could fuse with both the 5' end and the 3' end of viral RNA, were identified in the midgut of BmCPV-infected silkworms by RNA_seq and further confirmed by RT-PCR and Sanger sequencing. A novel chimeric RNA, HDAC11-S4 RNA 4, derived from silkworm histone deacetylase 11 (HDAC11) and the BmCPV S4 transcript encoding viral structural protein 4 (VP4), was selected for validation by in situ hybridization and Northern blotting. Interestingly, our results indicated that HDAC11-S4 RNA 4 was generated in a BmCPV RNA-dependent RNA polymerase (RdRp)-independent manner and could be translated into a truncated BmCPV VP4 with a silkworm HDAC11-derived N-terminal extension. Moreover, it was confirmed that HDAC11-S4 RNA 4 inhibited BmCPV proliferation, decreased the level of H3K9me3 and increased the level of H3K9ac. These results indicated that during infection with BmCPV, a novel mechanism, different from that described in previous reports, allows the genesis of chimeric silkworm-BmCPV RNAs with biological functions. Author summary: It has previously been reported that start codons with cap-snatched host transcripts or trans-splicing can generate chimeric RNAs on ssRNA and dsDNA viruses; however, whether chimeric RNAs can be generated on dsRNA viruses remains a mystery. We identified host-virus chimeric RNAs in a dsRNA virus infection model (BmCPV in silkworm), which is a novel phenomenon. What's more, our results indicated that the selected chimeric RNA HDAC11-S4 RNA 4 (derived from silkworm histone deacetylase 11 (HDAC11) and the BmCPV S4 transcript encoding viral structural protein 4 (VP4)) was generated in a BmCPV RNA-dependent RNA polymerase (RdRp)-independent manner and could be translated into a truncated BmCPV VP4 with a silkworm HDAC11-derived N-terminal extension. Further, We found that the chimeric RNA inhibited virus proliferation and decreased histone methylation, and increased histone acetylation. That is to say, the chimeric protein has activity and provides novel functionality compared to either of the normal versions of the two components on their own. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

34. Identification and characterization of extrachromosomal circular DNA in the silk gland of Bombyx mori.

Author

Zhu, Min, Tong, Xinyu, Qiu, Qunnan, Pan, Jun, Wei, Shulin, Ding, Yuming, Feng, Yongjie, Hu, Xiaolong, and Gong, Chengliang

Subjects
EXTRACHROMOSOMAL DNA, SILKWORMS, CIRCULAR DNA, KARYOKINESIS, PALINDROMIC DNA
Abstract

The silk gland cells of silkworm are special cells which only replicate DNA in the nucleus without cell division throughout the larval stage. The extrachromosomal circular DNAs (eccDNAs) have not yet been reported in the silk gland of silkworms. Herein, we have explored the characterization of eccDNAs in the posterior silk gland of silkworms. A total of 35 346 eccDNAs were identified with sizes ranging from 30 to 13 569 549 bp. Motif analysis revealed that dual direct repeats are flanking the 5′ and 3′ breaking points of eccDNA. The sequences exceeding 1 kb length in eccDNAs present palindromic sequence characteristics flanking the 5′ and 3′ breaking points of the eccDNA. These motifs might support possible models for eccDNA generation. Genomic annotation of the eccDNA population revealed that most eccDNAs (58.6%) were derived from intergenic regions, whereas full or partial genes were carried by 41.4% of eccDNAs. It was found that silk protein genes fib‐H, fib‐L, and P25, as well as the transcription factors SGF and sage, which play an important regulatory role in silk protein synthesis, could be carried by eccDNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that the genes carried by eccDNAs were mainly associated with the development and metabolism‐related signaling pathways. Moreover, it was found that eccDNAfib‐L could promote the transcription of fib‐L gene. Overall, the results of the present study not only provide a novel perspective on the mechanism of silk gland development and silk protein synthesis but also complement previously reported genome‐scale eccDNA data supporting that eccDNAs are common in eukaryotes. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results