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The study included 105 women of a reproductive age with chronic nonspecific salpingooophoritis. The women were transcerebrally exposed to interference currents (IC). The immunological examination revealed that IC have an immunocorrecting effect on both cellular and humoral links of the immune system, correct imbalance of the immunoregulatory subpopulations confirmed by increased immunoregulatory index. Inclusion of balneotherapy potentiates analgetic, anti-inflammatory effects of transcerebral IC therapy, regulates balance of immunoregulatory T-cell subpopulations due to better differentiation of T-lymphocytes in the thymus.

The proposed technique of cryomassage in outpatient setting promotes a complete healing of duodenal ulcer (DU) in patients with DU exacerbation. Healing of the ulcer is accompanied by improvement of blood flow in the zones of segmental innervation of the gastroduodenal system and by recovery of impaired cellular and humoral immunity in most of the patients.

Investigations have proved the ability of interauricular low-intensity infrared laser therapy (0.89 nm, 7.6 J/cm) to produce anti-inflammatory, immunomodulating action in patients with rheumatoid arthritis. The method has selective, pathogenetically directed immunomodulating effect the mechanism of which is similar to that of basic antirheumatic drugs and of intravenous laser radiation of blood. This laser therapy can be used as an alternative to intravenous blood radiation being superior as a noninvasive method. Interauricular laser therapy can potentiate the effects of nonsteroid anti-inflammatory drugs, cytostatics and diminish their side effects.

A trial of dry air effervescent baths and low-temperature peloid applications was made in 109 patients with psoriatic arthritis (PA). Marked response was achieved in patients who had received combined therapy with the above two modalities. The effect presented with lower articular index, weaker skin involvement, improvement of blood picture. These positive changes correlated with normalization of immune indices. The benefit persisted for 6-9 months.

The studies of 116 females with chronic nonspecific salpingo-oophoritis demonstrate that combination of low-frequency magnetic field with iodine-bromine water effectively corrects immunological indices, i.e. improves T- and B-cell immunity, production of IgM and IgA, proportion of immunoregulatory subpopulations of T-lymphocytes.

Low-frequency magnetic field generated by the vaginal inductor used in 120 females with chronic genital inflammation promoted a decrease in leukocytosis, elevation of total population of T-lymphocytes, inhibition of high proliferative activity in PHA test. However, marked immunocorrection was not reached.

80 patients with chronic bronchitis took baths with sodium chloride concentration 20, 40, 60 g/l and temperature 37-38 degrees C. The baths produced a positive effect on central and regional hemodynamics, reduced inflammation and sensitization.

The level of estrogen receptors (ER) in the cytosol and nuclear subfractions of female rat hepatocytes was studied 1 h and plasma angiotensinogen (AG) concentration 24 h after single and multiple administration of different doses of estradiol (E2) and synthetic estrogens. Synthetic weakly metabolized estrogens, used at doses corresponding to physiological concentrations of the natural female sex steroid, were shown to be much more effective than E2 in relation to ER redistribution between the cytosol and nuclear fractions of hepatocytes as well as in relation to the stimulation of AG production by the liver. Differences in the ER level in hepatocytic nuclei 1 h after single or multiple administration of the same estrogen were undetectable. An increase in a plasma AG level after a single injection of estrogens was noted after achieving a certain threshold (more than 3-fold as compared to the normal level) level of ER accumulation in hepatocytic nuclei. The sensitivity of AG production by the liver to a stimulating effect of low doses of estrogens was on the increase as a result of their repeated effect in prolonged administration and combined administration of E2 and glucocorticoids.

A comparative in vitro study of interaction of estradiol (E2) complexes with different forms of cytosol estrogen receptors (ER) from rat liver, kidney and uterus, and cell nuclei was carried out. It was shown that complexes of E2 with ER of whole liver and kidney cytosol of female rats as well as complexes of E2 with forms I and II of liver ER separated from one another and partially purified can be translocated into cell nuclei, similar to the estrogen-receptor complexes (ERC) of uterine cytosol. ERC, which contain an unusual estrogen-binding protein of male rat liver cytosol, do not bind to the nuclei in vitro. The translocation into the nuclei of high molecular weight ERC from liver and uterus has some common features, e. g. the necessity of activation by various factors, similar kinetics, absence of marked organ specificity, non-saturation of nuclear binding sites for ERC, etc. In terms of these criteria (with the exception of kinetic patterns) the translocation of forms I and II of female rat liver ER appears to be identical. The kinetic patterns and the level of translocation into the nuclei and the binding to DNA-cellulose of forms I and II of liver ER at low ionic strength reveal some differences, i. e. form II is characterized by faster kinetics, but by a lower level of interaction with the nuclei and DNA-cellulose. These differences disappear as the ionic strength of solution rises. The fast kinetics of ERC translocation into the nuclei at low ionic strength is also typical for kidney cytosol ER which are similar to those of form II of liver ER. The data obtained suggest that various forms of ER are involved in initiation of a broad spectrum of hormonal effects of Er both in the cells of different tissues and in homologous cells of the same tissue.

The trend in immunobiological responses to transcerebral action of decimeter microwaves was studied in intact rabbits. Transcerebral microwaves (120 mW/cm2) can alter functional activity of certain links of endocrine system due to possible activation of hypothalamus and hypophysis exposed to microwave electromagnetic field. Such exposure given in courses at early stages leads to an increase in the number of antibody-forming cells in the spleen, whereas total number of splenocytes is on the decrease.

The authors studied the effects of different doses of estradiol (E2) on the level of estrogen receptors (ER) in female rat hepatocytes and the dynamics of ER distribution between the cytosol and nuclear cell fractions and compared the changes in the ER level in the liver in different endocrine states of the body. It was shown that the ER level in hepatocytes was far lower than in uterine cells and drastically increased during puberation and after ovariectomy. It was also found that the ER level in hepatocytes was dependent on pituitary functions. After a single injection of E2, the ER level in cytosol descended and that in the nucleus rose. The degree of ER reduction in cytosol and increase in the nucleus correlated with the dose of E2. The dynamics of intercombined changes in the levels of cytosol and nuclear ER was studied at different time intervals following the injection of different doses of the hormone. Some essential organ-specific features of E2 reception in the liver were revealed. Different mechanisms of the control of ER content in the liver and uterus are postulated.

Interaction between cell nuclei and the complexes of estradiol (E2) with uterine cytosol estrogen receptors (ER) or with different forms of female and male rat liver cytosol ER was studied and compared. The complexes of E2 with forms I and II of liver ER are capable to translocate to the cell nuclei similarly to E2 receptor complexes of the uterus. The complexes of E2 with an unusual male rat liver estrogen-binding protein do not bind to the nuclei. The processes of translocation of the complexes of E2 with form I of liver ER and uterine receptors have some features in common. In the conditions of low ionic strength media, the complexes of E2 with forms I and II of liver ER manifest differences in the kinetics and level of translocation into the cell nuclei. The differences disappearing with ionic strength increase. The differences disappearing with ionic strength increase. It is suggested that the different forms of liver ER participate in the realization of E2 multiple effects in the liver.

The effects of estradiol (E2) and some other endocrine factors on rat plasma angiotensinogen (AG) have been studied. AG was determined by RIA. Both single and multiple injections of E2 to female rats were shown to cause a dose-dependent increase in AG in the blood. The effect of E2 was inhibited by cycloheximide. Multiple injections of E2 to male rats caused the same hepatic AG synthesis induction as those to female rats. Unlike E2, long-term treatment with large doses of testosterone propionate did not affect AG synthesis. AG levels in blood plasma of ovariectomized and immature female rats did not differ from those seen in mature intact rats. However, immature females were insensitive to the stimulant effect of the pharmacological doses of E2. Drastic reduction in hepatocyte sensitivity of the stimulant effect of E2 was seen in hypophysectomized female rats as well. Treatment of hypophysectomized animals with human GH, which did not affect AG levels per se, caused an almost complete recovery of hepatocyte ability to react by increasing AG synthesis in response to E2 injection. The possible role of different hormonal and genetic hepatotrophic factors in hepatocyte reactivity to the stimulant effect of E2 on AG synthesis is discussed.

A study was made of the level of hepatocyte estrogen receptors (ER) and of angiotensinogen [AG) in the blood plasma of rats after the administration of different doses of estradiol (E2) in combination with the exposure to some other endocrine factors. A single administration of E2 has been shown to cause two types of effects depending of the dose of a hormone administered and the time following injection: an increase in the AG level in the plasma and interrelated changes of the ER content in the cytosol and hepatocyte nuclei. These effects were most noticeable after the administration of 500 micrograms of E2. In this case the ER content decreased significantly in the cytosol and increased in the cell nucleus. An elevated ER level in the nucleus was preserved for 6-24 h and at this period AG production in the hepatocytes occurred and gradually increased within 24 h. An increase in AG production and the level and time of ER delay in the nuclei (r = +0.75, P less than 0.01) showed a high direct correlation. The expression of the effect of E2 high doses inducing AG synthesis depended on the body endocrine status which in its turn determined a certain level of E2 reception in hepatocytes. A low level of ER in the hepatocytes of hypophysectomized or immature rats corresponded to the absence or suppression of the E2 effect on AG production in the liver. An increase in the ER concentration in the cell as a result of the growth hormone administration to hypophysectomized or mature female rats was accompanied by the appearance or a rise of the sensitivity to the E2 effect.(ABSTRACT TRUNCATED AT 250 WORDS)