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1. Epidemiological and clinical characterization of community, healthcare-associated and nosocomial colonization and infection due to carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in Spain

Author

Salamanca-Rivera, Elena, Palacios-Baena, Zaira R., Cañada, Javier E., Moure, Zaira, Pérez-Vázquez, María, Calvo-Montes, Jorge, Martínez-Martínez, Luis, Cantón, Rafael, Ruiz Carrascoso, Guillermo, Pitart, Cristina, Navarro, Ferran, Bou, Germán, Mulet, Xavier, González-López, Juan José, Sivianes, Fran, Delgado-Valverde, Mercedes, Pascual, Álvaro, Oteo-Iglesias, Jesús, and Rodríguez-Baño, Jesús

Published
2024
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2. Synergistic impact of innate immunity hyper-activation and endothelial dysfunction on the magnitude of organ failure in the infection-sepsis continuum

Author

de la Fuente, Amanda, López-Sánchez, Jaime, Vaquero-Roncero, Luis Mario, Merino García, María, Sánchez Barrado, María Elisa, Sánchez-Hernández, Miguel Vicente, Garcia-Mateo, Nadia, Rico-Feijoo, Jesús, Muñoz-Bellvís, Luis, González de Castro, Rafael, Tedim, Ana P., Ortega, Alicia, Abdel-lah Fernández, Omar, Suárez-de-la-Rica, Alejandro, Maseda, Emilio, Trejo González, Ignacio, García Carrera, Geovanna Liszeth, Marcos-Vidal, José Miguel, Nieto Arranz, Juan Manuel, Chiscano-Camón, Luis, Ferrer, Ricard, Ruiz-Rodríguez, Juan Carlos, González-López, Juan José, Vila Fernández, José Alberto, Prieto Carballo, Regina, Lopez-Izquierdo, Raul, Garrosa, Sonsoles, Barón, Beatriz, Esteban-Velasco, Carmen, Aldecoa, César, and Bermejo-Martin, Jesús F.

Published
2024
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3. Enterobacterales carrying chromosomal AmpC β-lactamases in Europe (EuESCPM): Epidemiology and antimicrobial resistance burden from a cohort of 27 hospitals, 2020–2022

Author

Boattini, Matteo, Bianco, Gabriele, Llorente, Laura Iglesias, Acero, Laura Alonso, Nunes, Daniel, Seruca, Miguel, Mendes, Vasco Santos, Almeida, André, Bastos, Paulo, Rodríguez-Villodres, Ángel, Gascón, Adelina Gimeno, Halperin, Ana Verónica, Cantón, Rafael, Escartín, Maria Nieves Larrosa, González-López, Juan José, Floch, Pauline, Massip, Clémence, Chainier, Delphine, Barraud, Olivier, Dortet, Laurent, Cuzon, Gaëlle, Zancanaro, Clément, Mizrahi, Assaf, Schade, Rogier, Rasmussen, Asger Nellemann, Schønning, Kristian, Hamprecht, Axel, Schaffarczyk, Lukas, Glöckner, Stefan, Rödel, Jürgen, Kristóf, Katalin, Balonyi, Ágnes, Mancini, Stefano, Quiblier, Chantal, Fasciana, Teresa, Giammanco, Anna, Paglietti, Bianca, Rubino, Salvatore, Budimir, Ana, Bedenić, Branka, Rubic, Zana, Marinović, Jelena, Gartzonika, Konstantina, Christaki, Eirini, Mavromanolaki, Viktoria Eirini, Maraki, Sofia, Yalçın, Tuğba Yanık, Azap, Özlem Kurt, Licker, Monica, Musuroi, Corina, Talapan, Daniela, Vrancianu, Corneliu Ovidiu, Comini, Sara, Zalas-Więcek, Patrycja, Michalska, Anna, Cavallo, Rossana, Melo Cristino, José, and Costa, Cristina

Published
2024
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4. Genomic evolution of human respiratory syncytial virus during a decade (2013–2023): bridging the path to monoclonal antibody surveillance

Author

Piñana, Maria, González-Sánchez, Alejandra, Andrés, Cristina, Vila, Jorgina, Creus-Costa, Anna, Prats-Méndez, Ignasi, Arnedo-Muñoz, Maria, Saubi, Narcís, Esperalba, Juliana, Rando, Ariadna, Nadal-Baron, Patricia, Quer, Josep, González-López, Juan José, Soler-Palacín, Pere, Martínez-Urtaza, Jaime, Larrosa, Nieves, Pumarola, Tomàs, and Antón, Andrés

Published
2024
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5. The prognostic impact of SIGLEC5-induced impairment of CD8+ T cell activation in sepsis

Author

Lozano-Rodríguez, Roberto, Avendaño-Ortíz, José, Montalbán-Hernández, Karla, Ruiz-Rodríguez, Juan Carlos, Ferrer, Ricardo, Martín-Quirós, Alejandro, Maroun-Eid, Charbel, González-López, Juan José, Fàbrega, Anna, Terrón-Arcos, Verónica, Gutiérrez-Fernández, María, Alonso-López, Elisa, Cubillos-Zapata, Carolina, Fernández-Velasco, María, Pérez de Diego, Rebeca, Pelegrin, Pablo, García-Palenciano, Carlos, Cueto, Francisco J., del Fresno, Carlos, and López-Collazo, Eduardo

Published
2023
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7. Correction: Kirkegaard-Biosca et al. Cefiderocol for the Treatment of Infections by VIM-Type-Producing Gram-Negative Bacteria. Antibiotics 2024, 13 , 874.

Author

Kirkegaard-Biosca, Cristina, del Barrio-Tofiño, Ester, Villamarín, Miguel, Larrosa, Nieves, Campany, David, González-López, Juan José, Ferrer, Ricard, Viñado, Belén, Doménech, Laura, Sellarès-Nadal, Julia, Escolà-Vergé, Laura, Fernández-Hidalgo, Nuria, and Los-Arcos, Ibai

Subjects
HEMATOPOIETIC stem cell transplantation, CHRONIC obstructive pulmonary disease, URINARY tract infections, SURGICAL stents, EXTRACORPOREAL membrane oxygenation
Abstract

The correction notice for the article "Cefiderocol for the Treatment of Infections by VIM-Type-Producing Gram-Negative Bacteria" in the journal Antibiotics highlights an error in Table 2, specifically regarding patient outcomes. The corrected version of Table 2 is provided, with adjustments made to patient 23 and 24's mortality status at day 30. The authors assure that these corrections do not impact the scientific conclusions of the study, and the original publication has been updated accordingly. [Extracted from the article]

Published
2025
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8. Ciprofloxacin for the Treatment of Infections Caused by Carbapenemase-Producing Gram-Negative Bacteria †.

Author

Rubiñan, Pablo, Viñado, Belén, Fernández-Hidalgo, Nuria, Larrosa, Nieves, Sempere, Abiu, Campany, David, Rodríguez-Pardo, Dolors, González-López, Juan José, Nuvials, Xavier, del Barrio-Tofiño, Ester, Escolà-Vergé, Laura, and Los-Arcos, Ibai

Subjects
SOFT tissue infections, URINARY tract infections, INTRA-abdominal infections, SEPTIC shock, GRAM-negative bacteria
Abstract

Background: There is no experience with ciprofloxacin for the treatment of carbapenemase-producing Gram-negative bacteria (CP-GNB) infections. Methods: This is a retrospective single-centre study where we describe the clinical evolution of all consecutive adult patients who received ciprofloxacin monotherapy for the treatment of CP-GNB infections. Primary outcomes were clinical failure (defined as death, lack of clinical improvement or a switch to another drug) at day 14 and 30-day all-cause mortality. Results: Nineteen patients were included. Fifteen (79%) were men, the median age was 74 years (IQR 66–79) and the median Charlson comorbidity index was five (IQR 3–6.5). The most frequent infections were: nine complicated urinary tract infections, three soft tissue infections and three intra-abdominal infections. Twenty CP-GNBs were isolated (one patient had a coinfection): nine VIM-type-producing Enterobacterales, nine OXA-48-type-producing Enterobacterales and two VIM-type-producing Pseudomonas aeruginosa. Six (32%) patients had positive blood cultures, and one presented with septic shock. The median duration of ciprofloxacin treatment was 14 days (IQR 10–15). One patient presented with clinical failure at day 14. There was no 30-day mortality. Two patients exhibited microbiological recurrence at day 90. There were no reported adverse effects. Conclusions: Monotherapy with ciprofloxacin may be an alternative treatment for selected, clinically stable patients with ciprofloxacin-susceptible CP-GNB infections. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Circulation of multi-drug-resistant Shigella sonnei and Shigella flexneri among men who have sex with men in Barcelona, Spain, 2015–2019

Author

Moreno-Mingorance, Albert, Espinal, Paula, Rodriguez, Virginia, Goterris, Lidia, Fàbrega, Anna, Serra-Pladevall, Judit, Barberà, M. Jesús, Alberny, Mireia, Martín-González, Héctor, Cornejo-Sánchez, Thais, Armas, Mayuli, Mir-Cros, Alba, Raventós, Alex, Viñado, Belén, Pumarola, Tomàs, Larrosa, M. Nieves, and González-López, Juan José

Published
2021
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14. Use of teicoplanin monotherapy for the treatment of enterococcal infective endocarditis: a retrospective and comparative study at a referral centre.

Author

Villamarín, Miguel, Fernández-Hidalgo, Nuria, Viñado, Belén, González-López, Juan José, Rello, Pau, and Escolà-Vergé, Laura

Subjects
INFECTIVE endocarditis, HOSPITAL mortality, PARENTERAL therapy, TEICOPLANIN, MEDICAL referrals, ENTEROCOCCAL infections
Abstract

Objectives Clinical experience in the use of teicoplanin for treating enterococcal infective endocarditis (EIE) is scarce. The aim of this study was to describe the characteristics and outcomes of patients with EIE treated with teicoplanin monotherapy compared to standard therapy with ampicillin plus ceftriaxone. Methods All consecutive adult patients diagnosed with EIE between January 2018 and September 2022 at a referral centre were reviewed. Characteristics of individuals treated with teicoplanin for ≥14 days [the treated with teicoplanin (TT) group] were compared with those who received ampicillin plus ceftriaxone (AC group). Results Sixty-six patients were included [61 (92%) E. faecalis infective endocarditis (IE) and 5 (8%) E. faecium IE]. Twenty-seven (41%) received teicoplanin: eight as first-line treatment and 19 as continuation therapy. The median duration of teicoplanin treatment was 30 (25–43) days. Surgery was indicated in 14/27 (52%) in the TT group and in 21/39 (54%) in the AC group, but was finally performed in 11/14 (79%) and 13/21 (62%) (P  = 0.46), respectively. In-hospital mortality rate was 3/27 (11%) in the TT group and 12/39 (31%) in the AC group (P  = 0.06). Patients treated with teicoplanin were more often discharged on outpatient parenteral antibiotic therapy [18/27 (67%) versus 6/39 (15%), P  < 0.001] and median hospital stay was shorter [29 days (IQR 20–61) versus 50 days (IQR 43–68), P  = 0.006]. One-year cumulative mortality was 8/27 (30%) in the TT group and 13/39 (33%) in the AC group (P  = 0.46). There was one relapse in each group. Conclusion Teicoplanin seems an effective treatment for selected patients with enterococcal IE, mainly to facilitate discharge. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Pertussis Outbreak During 2023 in Gipuzkoa, North Spain.

Author

Marimón, José María, Montes, Milagrosa, Vizuete, Nahikari, Alvarez Guerrico, Lorea, Aginagalde, Adrian Hugo, Mir-Cros, Alba, González-López, Juan José, and Vicente, Diego

Subjects
BORDETELLA pertussis, COVID-19 pandemic, WHOOPING cough, WHOOPING cough vaccines, AGE groups
Abstract

Background: Pertussis has re-emerged in many countries despite the wide use of vaccines for over 60 years. During 2023, we observed an increase in the incidence of pertussis in Gipuzkoa, north of Spain (with a population of 657,140 inhabitants), mainly affecting children between 11 and 15 years of age. Methods: This study included all confirmed cases diagnosed by PCR in nasopharyngeal swab samples. The genome of seven isolates collected in 2023 was sequenced. Results: Between 2018 and 2023, 884 cases of whooping cough were diagnosed. Pertussis incidence (in cases per 100,000 inhabitants) decreased from 36.7 in 2018 to no cases in 2021, increasing again to 56.8 in 2023. In 2023, the age group of 11–15 years old had the highest incidence rate of 409.3. Only 2 of the 56 children < 6 years old required hospitalization, and there were no deaths. The seven isolates collected in 2023 showed the same BPagST-4 (ptxA1/ptxP3/prn2/fim2-1/fim3-1 allelic combination), with all of them expressing the pertactin antigen. Conclusions: Immunity waning after the last dose of vaccination at 6 years old, together with the lack of circulation of Bordetella pertussis during the COVID-19 pandemic, were probably the main reasons for the high increase in the incidence of pertussis in Gipuzkoa in 2023. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Cefiderocol for the Treatment of Infections by VIM-Type-Producing Gram-Negative Bacteria.

Author

Kirkegaard-Biosca, Cristina, del Barrio-Tofiño, Ester, Villamarín, Miguel, Larrosa, Nieves, Campany, David, González-López, Juan José, Ferrer, Ricard, Viñado, Belén, Doménech, Laura, Sellarès-Nadal, Julia, Escolà-Vergé, Laura, Fernández-Hidalgo, Nuria, and Los-Arcos, Ibai

Subjects
SOFT tissue infections, RESPIRATORY infections, GRAM-negative bacteria, MORTALITY, MEDICAL drainage
Abstract

VIM-type-producing Gram-negative bacteria (GNB) infections are difficult to treat. This is a retrospective single-center study of 34 patients who received cefiderocol for the treatment of VIM-type-producing GNB infections, including 25 Pseudomonas spp., 7 Enterobacterales, and 5 Achromobacter sp. Primary outcomes were clinical failure (defined as death, lack of clinical improvement, or a switch to another drug) at day 14 and 30-day all-cause mortality. The median age was 59 years (IQR 53.7–73.4), and the median Charlson comorbidity index was 3.5 (IQR 2–5). The main infections were respiratory tract infections (n = 9, 27%) and skin and soft tissue infections (n = 9, 27%). Eight patients exhibited bacteremia. In 9/17 patients with a drainable focus, drainage was performed. The median cefiderocol treatment duration was 13 days (IQR 8–24). Five patients (15%) experienced clinical failure on day 14, and the thirty-day mortality rate was 9/34 (27%); two cases occurred because of an uncontrolled infection source, and one was due to a new infection caused by the same bacteria. The other six deaths were unrelated to the index infection. Five patients experienced microbiological recurrence within three months. Susceptibility testing revealed the development of cefiderocol resistance in 1/7 cases with persistent or recurrent positive cultures. Cefiderocol, even in monotherapy, could be considered for the treatment of VIM-type-producing GNB infections. [ABSTRACT FROM AUTHOR]

Published
2024
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Author

Boattini, Matteo, Bianco, Gabriele, Llorente, Laura Iglesias, Acero, Laura Alonso, Nunes, Daniel, Seruca, Miguel, Mendes, Vasco Santos, Almeida, André, Bastos, Paulo, Rodríguez-Villodres, Ángel, Gascón, Adelina Gimeno, Halperin, Ana Verónica, Cantón, Rafael, Escartín, Maria Nieves Larrosa, González-López, Juan José, Floch, Pauline, Massip, Clémence, Chainier, Delphine, Barraud, Olivier, Dortet, Laurent, Cuzon, Gaëlle, Zancanaro, Clément, Mizrahi, Assaf, Schade, Rogier, Rasmussen, Asger Nellemann, Schønning, Kristian, Hamprecht, Axel, Schaffarczyk, Lukas, Glöckner, Stefan, Rödel, Jürgen, Kristóf, Katalin, Balonyi, Ágnes, Mancini, Stefano, Quiblier, Chantal, Fasciana, Teresa, Giammanco, Anna, Paglietti, Bianca, Rubino, Salvatore, Budimir, Ana, Bedenić, Branka, Rubic, Zana, Marinović, Jelena, Gartzonika, Konstantina, Christaki, Eirini, Mavromanolaki, Viktoria Eirini, Maraki, Sofia, Yalçın, Tuğba Yanık, Azap, Özlem Kurt, Licker, Monica, Musuroi, Corina, Talapan, Daniela, Vrancianu, Corneliu Ovidiu, Comini, Sara, Zalas-Więcek, Patrycja, Michalska, Anna, Cavallo, Rossana, Melo Cristino, José, Costa, Cristina, Boattini, Matteo, Bianco, Gabriele, Llorente, Laura Iglesias, Acero, Laura Alonso, Nunes, Daniel, Seruca, Miguel, Mendes, Vasco Santos, Almeida, André, Bastos, Paulo, Rodríguez-Villodres, Ángel, Gascón, Adelina Gimeno, Halperin, Ana Verónica, Cantón, Rafael, Escartín, Maria Nieves Larrosa, González-López, Juan José, Floch, Pauline, Massip, Clémence, Chainier, Delphine, Barraud, Olivier, Dortet, Laurent, Cuzon, Gaëlle, Zancanaro, Clément, Mizrahi, Assaf, Schade, Rogier, Rasmussen, Asger Nellemann, Schønning, Kristian, Hamprecht, Axel, Schaffarczyk, Lukas, Glöckner, Stefan, Rödel, Jürgen, Kristóf, Katalin, Balonyi, Ágnes, Mancini, Stefano, Quiblier, Chantal, Fasciana, Teresa, Giammanco, Anna, Paglietti, Bianca, Rubino, Salvatore, Budimir, Ana, Bedenić, Branka, Rubic, Zana, Marinović, Jelena, Gartzonika, Konstantina, Christaki, Eirini, Mavromanolaki, Viktoria Eirini, Maraki, Sofia, Yalçın, Tuğba Yanık, Azap, Özlem Kurt, Licker, Monica, Musuroi, Corina, Talapan, Daniela, Vrancianu, Corneliu Ovidiu, Comini, Sara, Zalas-Więcek, Patrycja, Michalska, Anna, Cavallo, Rossana, Melo Cristino, José, and Costa, Cristina

Published
2024

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Author

Boattini, Matteo, Bianco, Gabriele, Llorente, Laura Iglesias, Acero, Laura Alonso, Nunes, Daniel, Seruca, Miguel, Mendes, Vasco Santos, Almeida, André, Bastos, Paulo, Rodríguez-Villodres, Ángel, Gascón, Adelina Gimeno, Halperin, Ana Verónica, Cantón, Rafael, Escartín, Maria Nieves Larrosa, González-López, Juan José, Floch, Pauline, Massip, Clémence, Chainier, Delphine, Barraud, Olivier, Dortet, Laurent, Cuzon, Gaëlle, Zancanaro, Clément, Mizrahi, Assaf, Schade, Rogier, Rasmussen, Asger Nellemann, Schønning, Kristian, Hamprecht, Axel, Schaffarczyk, Lukas, Glöckner, Stefan, Rödel, Jürgen, Kristóf, Katalin, Balonyi, Agnes, Mancini, Stefano; https://orcid.org/0000-0003-4516-5952, Quiblier, Chantal, et al, Boattini, Matteo, Bianco, Gabriele, Llorente, Laura Iglesias, Acero, Laura Alonso, Nunes, Daniel, Seruca, Miguel, Mendes, Vasco Santos, Almeida, André, Bastos, Paulo, Rodríguez-Villodres, Ángel, Gascón, Adelina Gimeno, Halperin, Ana Verónica, Cantón, Rafael, Escartín, Maria Nieves Larrosa, González-López, Juan José, Floch, Pauline, Massip, Clémence, Chainier, Delphine, Barraud, Olivier, Dortet, Laurent, Cuzon, Gaëlle, Zancanaro, Clément, Mizrahi, Assaf, Schade, Rogier, Rasmussen, Asger Nellemann, Schønning, Kristian, Hamprecht, Axel, Schaffarczyk, Lukas, Glöckner, Stefan, Rödel, Jürgen, Kristóf, Katalin, Balonyi, Agnes, Mancini, Stefano; https://orcid.org/0000-0003-4516-5952, Quiblier, Chantal, and et al

Published
2024

21. Catastrophic Streptococcus pyogenes Disease: A Personalized Approach Based on Phenotypes and Treatable Traits

Author

Ruiz-Rodríguez, Juan Carlos, primary, Chiscano-Camón, Luis, additional, Maldonado, Carolina, additional, Ruiz-Sanmartin, Adolf, additional, Martin, Laura, additional, Bajaña, Ivan, additional, Bastidas, Juliana, additional, Lopez-Martinez, Rocio, additional, Franco-Jarava, Clara, additional, González-López, Juan José, additional, Ribas, Vicent, additional, Larrosa, Nieves, additional, Riera, Jordi, additional, Nuvials-Casals, Xavier, additional, and Ferrer, Ricard, additional

Published
2024
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30. Clinical, microbiological, and molecular characterization of pediatric invasive infections by Streptococcus pyogenes in Spain in a context of global outbreak.

Author

Ramírez de Arellano, Eva, Saavedra-Lozano, Jesús, Villalón, Pilar, Jové-Blanco, Ana, Grandioso, David, Sotelo, Jared, Gamell, Anna, González-López, Juan José, Cervantes, Eloísa, Gónzalez, María José, Rello-Saltor, Victoria, Esteva, Cristina, Sanz-Santaeufemia, Francisco, Yagüe, Genoveva, Manzanares, Ángela, Brañas, Patricia, Ruiz de Gopegui, Enrique, Carrasco-Colom, Jaime, García, Federico, and Cercenado, Emilia

Published
2024
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31. Use of Biomarkers to Improve 28-Day Mortality Stratification in Patients with Sepsis and SOFA ≤ 6

Author

Baldirà, Jaume, primary, Ruiz-Rodríguez, Juan Carlos, additional, Ruiz-Sanmartin, Adolfo, additional, Chiscano, Luis, additional, Cortes, Alejandro, additional, Sistac, Diego Ángeles, additional, Ferrer-Costa, Roser, additional, Comas, Inma, additional, Villena, Yolanda, additional, Larrosa, Maria Nieves, additional, González-López, Juan José, additional, and Ferrer, Ricard, additional

Published
2023
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32. Use of Biomarkers to Improve 28-Day Mortality Stratification in Patients with Sepsis and SOFA≤6

Author

Baldirà, Jaume, primary, Ruiz-Rodríguez, Juan Carlos, additional, Ruiz-Sanmartin, Adolfo, additional, Chiscano, Luis, additional, Cortés, Alejandro, additional, Angeles-Sistac, Diego, additional, Ferrer-Costa, Roser, additional, Comas, Imma, additional, Villena, Yolanda, additional, Larrosa, M.Nieves, additional, González-López, Juan José, additional, and Ferrer, Ricard, additional

Published
2023
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34. Comprehensive clinical and epidemiological assessment of colonisation and infection due to carbapenemase-producing Enterobacteriaceae in Spain

Author

Zaballos, Ángel, Cantón, Rafael, Martínez-García, Laura, Fleites, Ana María, Rodríguez-Lucas, Carlos, Sánchez-Romero, M<ce:sup loc='post">a</ce:sup> Isabel, García-Picazo, Luisa, Aznar, Esteban, Campelo, Carolina, González-Praetorius, Alejandro, Solís, Sonia, Giner, Salvador, Salavert, Miguel, Hernández, Juan Manuel, Pujals, Josep Vilaró, Bastarras, Anna Vilamala, Orellana, M<ce:sup loc='post">a</ce:sup> Ángeles, Cercenado, Emilia, Espasa, Mateu, Fontanals, Dionisia, García-López, M<ce:sup loc='post">a</ce:sup> Victoria, Hernández-Almaraz, José Luis, Martí-Sala, Carmina, Gimeno, Adelina, Alarcón, Teresa, Llorca, Laura, Segura, Concepción, Clivillé-Abad, Raquel, Motjé, Montse, Garcia i Parés, Delia, de la Iglesia, Pedro, Iglesias, Beatriz, Castón, Juanjo, Romero, María Dolores, Rodríguez-Polo, José Antonio, Trujillo, Gloria, Morta, Montserrat, Setas, Alberto Gil, Ezpeleta, Carmen, Miguel-Martínez, M<ce:sup loc='post">a</ce:sup> Dolores, Sánchez-Porto, Antonio, Casas, Javier, Molina, David, Garduño, Eugenio, Alados, Juan Carlos, Pérez-Jové, Pepa, Sauca, Goretti, Gallés, Carmen, Galánand, Fátima, Guerrero, Francisca, Brezmes, M<ce:sup loc='post">a</ce:sup> Fe, Ortega, M<ce:sup loc='post">a</ce:sup> Pilar, Castillo, Francisco Javier, Seral, Cristina, Delgado-Iribarren, Alberto, Yagüe, Alberto, Aspiroz, Carmen, Fernández-Natal, M<ce:sup loc='post">a</ce:sup> Isabel, Wilhelmi, Isabel, Reyes, Pilar, Pérez-Ramírez, M<ce:sup loc='post">a</ce:sup> Dolores, Cuesta, Inocente, Pérez Moreno, Mar Olga, García, Amparo, Ballester, Frederic, Pujol, Isabel, Sierra, Montserrat, González-Cuevas, Araceli, García, Pilar López, Saladrigas, Lluís Carbó, Martínez-López, Jesús, Martínez-Lamas, Lucía, Cabrera, Jorge Julio, de Cruz, Susana García, Raya, Carmen, Campo, Ana Belén, de Benito, Inés, Canut, Andrés, Berdonces, Pilar, Lecaroz Agara, María Concepción, Real, Susana Hernando, Hernández, Belén, Ledo, M<ce:sup loc='post">a</ce:sup> Teresa, El Knaichi, Firdaous, Tejero, Carlos García, Azcona, Jose Manuel, Ferrer, Isabel, Lamata, Marta, Pazos, Carmen, Chocarro, M<ce:sup loc='post">a</ce:sup> Pilar, Murillas, Javier, Miró, Elisenda, Navarro, Ferrán, Bartolomé, Rosa M., Palacios-Baena, Zaira R., Oteo, Jesús, Conejo, Carmen, Larrosa, M. Nieves, Bou, Germán, Fernández-Martínez, Marta, González-López, Juan José, Pintado, Vicente, Martínez-Martínez, Luis, Merino, María, Pomar, Virginia, Mora-Rillo, Marta, Rivera, María Alba, Oliver, Antonio, Ruiz-Carrascoso, Guillermo, Ruiz-Garbajosa, Patricia, Zamorano, Laura, Bautista, Verónica, Ortega, Adriana, Morales, Isabel, Pascual, Álvaro, Campos, José, and Rodríguez-Baño, Jesús

Published
2016
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36. CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3.

Author

Cañada-García, Javier E., Moure, Zaira, Sola-Campoy, Pedro J., Delgado-Valverde, Mercedes, Cano, María E., Gijón, Desirèe, González, Mónica, Gracia-Ahufinger, Irene, Larrosa, Nieves, Mulet, Xavier, Pitart, Cristina, Rivera, Alba, Bou, Germán, Calvo, Jorge, Cantón, Rafael, González-López, Juan José, Martínez-Martínez, Luis, Navarro, Ferran, Oliver, Antonio, and Palacios-Baena, Zaira R.

Subjects
KLEBSIELLA pneumoniae, MOLECULAR cloning, ESCHERICHIA coli, MICROBIAL sensitivity tests, SINGLE nucleotide polymorphisms, NUCLEOTIDE sequencing
Abstract

Objectives: CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain. Methods: In total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC > 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis. Results: In total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were blaOXA−48 (263/377), blaKPC−3 (62/377), blaVIM−1 (28/377), and blaNDM−1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5). Conclusion: This study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3. [ABSTRACT FROM AUTHOR]

Published
2023
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38. Increasing trend of antimicrobial resistance in Shigella associated with MSM transmission in Barcelona, 2020–21: outbreak of XRD Shigella sonnei and dissemination of ESBL-producing Shigella flexneri

Author

Moreno-Mingorance, Albert, primary, Mir-Cros, Alba, additional, Goterris, Lidia, additional, Rodriguez-Garrido, Virginia, additional, Sulleiro, Elena, additional, Barberà, M Jesús, additional, Alberny, Mireia, additional, Hoyos-Mallecot, Yannick, additional, Descalzo, Vicente, additional, Bravo, Albert, additional, Roca-Grande, Josep, additional, Viñado, Belén, additional, Pumarola, Tomàs, additional, Larrosa, M Nieves, additional, and González-López, Juan José, additional

Published
2023
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39. Complex pathogens in infective endocarditis

Author

Escolà-Vergé, Laura, primary, Roque, Albert, additional, Pizzi, María Nazarena, additional, González-López, Juan José, additional, Almirante, Benito, additional, and Fernández-Hidalgo, Nuria, additional

Published
2023
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41. Molecular epidemiology and virulence of Escherichia coli O16:H5-ST131: Comparison with H30 and H30-Rx subclones of O25b:H4-ST131

Author

Dahbi, Ghizlane, Mora, Azucena, Mamani, Rosalia, López, Cecilia, Alonso, María Pilar, Marzoa, Juan, Blanco, Miguel, Herrera, Alexandra, Viso, Susana, García-Garrote, Fernando, Tchesnokova, Veronika, Billig, Mariya, de la Cruz, Fernando, de Toro, María, González-López, Juan José, Prats, Guillermo, Chaves, Fernando, Martínez-Martínez, Luis, López-Cerezo, Lorena, Denamur, Erick, and Blanco, Jorge

Published
2014
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42. Ceftazidime-Avibactam plus Aztreonam for the Treatment of Infections by VIM-Type-Producing Gram-Negative Bacteria

Author

Sempere, Abiu, primary, Viñado, Belén, additional, Los-Arcos, Ibai, additional, Campany, David, additional, Larrosa, Nieves, additional, Fernández-Hidalgo, Nuria, additional, Rodríguez-Pardo, Dolors, additional, González-López, Juan José, additional, Nuvials, Xavier, additional, Almirante, Benito, additional, and Escolà-Vergé, Laura, additional

Published
2022
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44. CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3

Author

Cañada García, Javier E., Moure, Zaira, Sola Campoy, Pedro J., Delgado Valverde, Mercedes, Cano, María E., Gijón, Desirèe, González, Mónica, Gracia Ahufinger, Irene, Larrosa, Nieves, Mulet, Xavier, Pitart, Cristina, Rivera, Alba, Bou, Germán, Calvo, Jorge, Cantón, Rafael, González-López, Juan José, Martínez-Martínez, Luis, Navarro, Ferran, Oliver, Antonio, Palacios Baena, Zaira R., Pascual, Alvaro, Ruiz Carrascoso, Guillermo, Vila Estapé, Jordi, Aracil, Belén, Pérez-Vázquez, María, Oteo Iglesias, Jesús, GEMARA/GEIRAS-SEIMC/REIPI CARB-ES-19 Study Group, Instituto de Salud Carlos III, Red Española de Investigación en Patología Infecciosa, European Commission, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (España), Universidad de Sevilla. Departamento de Microbiología, Universidad de Sevilla. CTS210: Resistencia a antimicrobianos., Ministerio de Economía y Competitividad (MINECO). España, Universidad de Cantabria, Institut Català de la Salut, [Cañada-García JE, Moure Z, Sola-Campoy PJ] Laboratorio de Referencia e Investigación en Resistencia a Antibióticos e Infecciones Relacionadas con la Asistencia Sanitaria, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain. [Delgado-Valverde M] Unidad de Enfermedades Infecciosas y Microbiología, Hospital Universitario Virgen Macarena, Instituto de Biomedicina de Sevilla (Hospital Universitario Virgen Macarena/CSIC/Universidad de Sevilla), Seville, Spain. CIBER de Enfermedades Infecciosas (CIBERINFEC), REIPI, Instituto de Salud Carlos III, Madrid, Spain. [Cano ME] Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, IDIVAL, Santander, Spain. [Gijón D] CIBER de Enfermedades Infecciosas (CIBERINFEC), REIPI, Instituto de Salud Carlos III, Madrid, Spain. Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain. [Larrosa N, González-López JJ] CIBER de Enfermedades Infecciosas (CIBERINFEC), REIPI, Instituto de Salud Carlos III, Madrid, Spain. Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain, Vall d'Hebron Barcelona Hospital Campus, Plan Nacional de I+D+i (España), Ministerio de Economía y Competitividad (España), Red de Investigación Cooperativa en Investigación en Patología Infecciosa (España), Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas), and Unión Europea

Subjects
Microbiology (medical), Enzimologia, Human genome, Bacteria::bacterias gramnegativas::bacilos gramnegativos anaerobios facultativos::Enterobacteriaceae::Escherichia::Escherichia coli [ORGANISMOS], acciones y usos químicos::acciones farmacológicas::usos terapéuticos::antiinfecciosos::antibacterianos [COMPUESTOS QUÍMICOS Y DROGAS], Otros calificadores::Otros calificadores::Otros calificadores::/enzimología [Otros calificadores], Carbapenemases, Genoma humà, Microbiology, Enterobacteriàcies, Klebsiella pneumoniae, Bacteria::Gram-Negative Bacteria::Gram-Negative Facultatively Anaerobic Rods::Enterobacteriaceae::Escherichia::Escherichia coli [ORGANISMS], Escheríchia coli, Bacteria::Gram-Negative Bacteria::Gram-Negative Facultatively Anaerobic Rods::Enterobacteriaceae::Klebsiella::Klebsiella pneumoniae [ORGANISMS], Enterobacteriaceae, Whole genome sequencing, Bacteria::bacterias gramnegativas::bacilos gramnegativos anaerobios facultativos::Enterobacteriaceae::Klebsiella::Klebsiella pneumoniae [ORGANISMOS], Escherichia coli, Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agents [CHEMICALS AND DRUGS], Antibiòtics betalactàmics, Medicaments antibacterians, CARB-ES-19 study, Other subheadings::Other subheadings::Other subheadings::/enzymology [Other subheadings], High-risk clones, Beta lactam antibiotics
Abstract

CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain., This research was supported by grants from the Instituto de Salud Carlos III (numbers PI18CIII/00030 and PI21CIII/00039). It was also supported by Plan Nacional de I + D + i 2013–2016, Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía y Competitividad, Spanish Network for Research in Infectious Diseases (grants RD16CIII/0004/0002, RD16/0016/0001, RD16/0016/0003, RD16/0016/0004, RD16/0016/0006, RD16/0016/0007, RD16/0016/0008, RD16/0016/0010, and RD16/0016/0011). Cofinanced by the European Development Regional Fund “A way to achieve Europe,” Operative Program Intelligent Growth 2014–2020. CIBER – Consorcio Centro de Investigación Biomédica en Red (CB21/13/00095, CB21/13/00012, CB21/13/00049, CB21/13/00054, CB21/13/00055, CB21/13/00068, CB21/13/00081, CB21/13/00084, and CB21/13/00099) (CIBERINFEC) and Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea – NextGenerationEU also supported this work.

Published
2022

45. Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology : Implementation and Clinical Validation

Author

Calvo Lozano, Olalla, Sierra, Miquel, Soler, María José, Estévez, M. Carmen, Chiscano-Camón, Luis, Ruiz-Sanmartin, Adolfo, Ruiz-Rodriguez, Juan Carlos, Ferrer, Ricard, González-López, Juan José, Esperalba, Juliana, Fernandez-Naval, Candela, Bueno, Leticia, López-Aladid, Ruben, Torres, Antoni, Fernández-Barat, Laia, Attoumani, Sarah, Charrel, Rémi, Coutard, Bruno, Lechuga, Laura, Calvo Lozano, Olalla, Sierra, Miquel, Soler, María José, Estévez, M. Carmen, Chiscano-Camón, Luis, Ruiz-Sanmartin, Adolfo, Ruiz-Rodriguez, Juan Carlos, Ferrer, Ricard, González-López, Juan José, Esperalba, Juliana, Fernandez-Naval, Candela, Bueno, Leticia, López-Aladid, Ruben, Torres, Antoni, Fernández-Barat, Laia, Attoumani, Sarah, Charrel, Rémi, Coutard, Bruno, and Lechuga, Laura

Abstract

Serological tests are essential for the control and management of COVID-19 pandemic (diagnostics and surveillance, and epidemiological and immunity studies). We introduce a direct serological biosensor assay employing proprietary technology based on plasmonics, which offers rapid (<15 min) identification and quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies in clinical samples, without signal amplification. The portable plasmonic device employs a custom-designed multiantigen (RBD peptide and N protein) sensor biochip and reaches detection limits in the low ng mL -1 range employing polyclonal antibodies. It has also been implemented employing the WHO-approved anti-SARS-CoV-2 immunoglobulin standard. A clinical validation with COVID-19 positive and negative samples (n = 120) demonstrates its excellent diagnostic sensitivity (99%) and specificity (100%). This positions our biosensor as an accurate and easy-to-use diagnostics tool for rapid and reliable COVID-19 serology to be employed both at laboratory and decentralized settings for the disease management and for the evaluation of immunological status during vaccination or treatment.

Published
2022

46. Table_4_CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3.pdf

Author

Cañada-García, Javier E, Moure, Zaira, Sola-Campoy, Pedro J., Delgado-Valverde, Mercedes, Cano, María Eugenia, Gijón, Desirèe, González-Bardanca, Mónica, Gracia-Ahufinger, Irene, Larrosa, Nieves, Mulet, Xavier, Pitart, Cristina, Rivera, Alba, Bou, Germán, Calvo-Montes, Jorge, Cantón, Rafael, González-López, Juan José, Martínez-Martínez, Luis, Navarro, Ferrán, Oliver, Antonio, Palacios-Baena, Zaira Raquel, Pascual, Álvaro, Ruiz Carrascoso, Guillermo, Vila, Jordi, Aracil, Belén, Pérez-Vázquez, María, Oteo-Iglesias, Jesús, Cañada-García, Javier E, Moure, Zaira, Sola-Campoy, Pedro J., Delgado-Valverde, Mercedes, Cano, María Eugenia, Gijón, Desirèe, González-Bardanca, Mónica, Gracia-Ahufinger, Irene, Larrosa, Nieves, Mulet, Xavier, Pitart, Cristina, Rivera, Alba, Bou, Germán, Calvo-Montes, Jorge, Cantón, Rafael, González-López, Juan José, Martínez-Martínez, Luis, Navarro, Ferrán, Oliver, Antonio, Palacios-Baena, Zaira Raquel, Pascual, Álvaro, Ruiz Carrascoso, Guillermo, Vila, Jordi, Aracil, Belén, Pérez-Vázquez, María, and Oteo-Iglesias, Jesús

Abstract

[Objectives] CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain., [Methods] In total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC > 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis., [Results] In total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were blaOXA–48 (263/377), blaKPC–3 (62/377), blaVIM–1 (28/377), and blaNDM–1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5)., [Conclusion] This study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3.

Published
2022

47. Image_1_CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3.pdf

Author

Cañada-García, Javier E, Moure, Zaira, Sola-Campoy, Pedro J., Delgado-Valverde, Mercedes, Cano, María Eugenia, Gijón, Desirèe, González-Bardanca, Mónica, Gracia-Ahufinger, Irene, Larrosa, Nieves, Mulet, Xavier, Pitart, Cristina, Rivera, Alba, Bou, Germán, Calvo-Montes, Jorge, Cantón, Rafael, González-López, Juan José, Martínez-Martínez, Luis, Navarro, Ferrán, Oliver, Antonio, Palacios-Baena, Zaira Raquel, Pascual, Álvaro, Ruiz Carrascoso, Guillermo, Vila, Jordi, Aracil, Belén, Pérez-Vázquez, María, Oteo-Iglesias, Jesús, Cañada-García, Javier E, Moure, Zaira, Sola-Campoy, Pedro J., Delgado-Valverde, Mercedes, Cano, María Eugenia, Gijón, Desirèe, González-Bardanca, Mónica, Gracia-Ahufinger, Irene, Larrosa, Nieves, Mulet, Xavier, Pitart, Cristina, Rivera, Alba, Bou, Germán, Calvo-Montes, Jorge, Cantón, Rafael, González-López, Juan José, Martínez-Martínez, Luis, Navarro, Ferrán, Oliver, Antonio, Palacios-Baena, Zaira Raquel, Pascual, Álvaro, Ruiz Carrascoso, Guillermo, Vila, Jordi, Aracil, Belén, Pérez-Vázquez, María, and Oteo-Iglesias, Jesús

Abstract

[Objectives] CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain., [Methods] In total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC > 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis., [Results] In total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were blaOXA–48 (263/377), blaKPC–3 (62/377), blaVIM–1 (28/377), and blaNDM–1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5)., [Conclusion] This study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3.

Published
2022

48. CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3

Author

Instituto de Salud Carlos III, Red Española de Investigación en Patología Infecciosa, European Commission, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (España), Cañada-García, Javier E, Moure, Zaira, Sola-Campoy, Pedro J., Delgado-Valverde, Mercedes, Cano, María Eugenia, Gijón, Desirèe, González-Bardanca, Mónica, Gracia-Ahufinger, Irene, Larrosa, Nieves, Mulet, Xavier, Pitart, Cristina, Rivera, Alba, Bou, Germán, Calvo-Montes, Jorge, Cantón, Rafael, González-López, Juan José, Martínez-Martínez, Luis, Navarro, Ferrán, Oliver, Antonio, Palacios-Baena, Zaira Raquel, Pascual, Álvaro, Ruiz Carrascoso, Guillermo, Vila, Jordi, Aracil, Belén, Pérez-Vázquez, María, Oteo-Iglesias, Jesús, Instituto de Salud Carlos III, Red Española de Investigación en Patología Infecciosa, European Commission, Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (España), Cañada-García, Javier E, Moure, Zaira, Sola-Campoy, Pedro J., Delgado-Valverde, Mercedes, Cano, María Eugenia, Gijón, Desirèe, González-Bardanca, Mónica, Gracia-Ahufinger, Irene, Larrosa, Nieves, Mulet, Xavier, Pitart, Cristina, Rivera, Alba, Bou, Germán, Calvo-Montes, Jorge, Cantón, Rafael, González-López, Juan José, Martínez-Martínez, Luis, Navarro, Ferrán, Oliver, Antonio, Palacios-Baena, Zaira Raquel, Pascual, Álvaro, Ruiz Carrascoso, Guillermo, Vila, Jordi, Aracil, Belén, Pérez-Vázquez, María, and Oteo-Iglesias, Jesús

Abstract

CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain.

Published
2022

49. Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology: Implementation and Clinical Validation

Author

European Commission, Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Consejo Superior de Investigaciones Científicas (España), Fundació Glòria Soler, European Virus Archive Global, Vall d'Hebron Research Institute, Instituto de Salud Carlos III, Calvo-Lozano, Olalla [0000-0001-5486-2237], Soler, María [0000-0001-7232-2277], Estévez, M. Carmen [0000-0003-3694-7186], Lechuga, Laura M. [0000-0001-5187-5358], Calvo-Lozano, Olalla, Sierra, Miquel, Soler, María, Estévez, M. Carmen, Chiscano- Camón, Luis, Ruiz-Sanmartín, Adolfo, Ruiz-Rodriguez, Juan Carlos, Ferrer, Ricard, González-López, Juan José, Esperalba, Juliana, Fernández-Nava, Candela, Bueno, Leticia, López-Aladid, Rubén, Torres, Antoni, Fernández-Barat, Laia, Attoumani, Sarah, Charrel, Remi, Coutard, Bruno, Lechuga, Laura M., European Commission, Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Consejo Superior de Investigaciones Científicas (España), Fundació Glòria Soler, European Virus Archive Global, Vall d'Hebron Research Institute, Instituto de Salud Carlos III, Calvo-Lozano, Olalla [0000-0001-5486-2237], Soler, María [0000-0001-7232-2277], Estévez, M. Carmen [0000-0003-3694-7186], Lechuga, Laura M. [0000-0001-5187-5358], Calvo-Lozano, Olalla, Sierra, Miquel, Soler, María, Estévez, M. Carmen, Chiscano- Camón, Luis, Ruiz-Sanmartín, Adolfo, Ruiz-Rodriguez, Juan Carlos, Ferrer, Ricard, González-López, Juan José, Esperalba, Juliana, Fernández-Nava, Candela, Bueno, Leticia, López-Aladid, Rubén, Torres, Antoni, Fernández-Barat, Laia, Attoumani, Sarah, Charrel, Remi, Coutard, Bruno, and Lechuga, Laura M.

Abstract

Serological tests are essential for the control and management of COVID-19 pandemic (diagnostics and surveillance, and epidemiological and immunity studies). We introduce a direct serological biosensor assay employing proprietary technology based on plasmonics, which offers rapid (<15 min) identification and quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies in clinical samples, without signal amplification. The portable plasmonic device employs a custom-designed multiantigen (RBD peptide and N protein) sensor biochip and reaches detection limits in the low ng mL–1 range employing polyclonal antibodies. It has also been implemented employing the WHO-approved anti-SARS-CoV-2 immunoglobulin standard. A clinical validation with COVID-19 positive and negative samples (n = 120) demonstrates its excellent diagnostic sensitivity (99%) and specificity (100%). This positions our biosensor as an accurate and easy-to-use diagnostics tool for rapid and reliable COVID-19 serology to be employed both at laboratory and decentralized settings for the disease management and for the evaluation of immunological status during vaccination or treatment.

Published
2022

50. Pertactin-Deficient Bordetella pertussis with Unusual Mechanism of Pertactin Disruption, Spain, 1986–2018

Author

Mir-Cros, Alba, primary, Moreno-Mingorance, Albert, additional, Martín-Gómez, M. Teresa, additional, Abad, Raquel, additional, Bloise, Iván, additional, Campins, Magda, additional, González-Praetorius, Alejandro, additional, Gutiérrez, M. Nieves, additional, Martín-González, Héctor, additional, Muñoz-Almagro, Carmen, additional, Orellana, M. Ángeles, additional, de Pablos, Manuela, additional, Roca-Grande, Josep, additional, Rodrigo, Carlos, additional, Rodríguez, M. Elena, additional, Uriona, Sonia, additional, Vidal, M. José, additional, Pumarola, Tomàs, additional, Larrosa, M. Nieves, additional, and González-López, Juan José, additional

Published
2022
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