Search

Abstract Aims We examined the prevalence and incidence of mental health symptoms (MHS) in active professional male footballers over a 12-month period and investigated if MHS was associated with severe injuries or surgeries. Participants and methods Football Players Worldwide (FIFPRO) affiliated national unions invited active professional male football players to participate in the study. MHS was operationalised in symptoms of anxiety, disordered eating, depression, distress, sleep disturbance, alcohol misuse and drug misuse, all being assessed with validated questionnaires. Results Of the 101 participants enrolled, the prevalence of distress was 53% and MHS was between 6% for drug misuse and 48% for alcohol misuse. The incidence of distress was 29% and MHS ranged from 1% for anxiety to 11% for sleep disturbance. At baseline, players suffering from injury/surgery were more likely to report depression (OR 1.35; 95%CI 1.10–1.70) and disordered eating (OR 1.22; 95%CI 1.02–1.47). At follow-up, players who suffered injury or surgery were inclined to report distress (OR 2.15; 95%CI 1.26–4.31) and drug misuse (OR 2.05; 95%CI 1.01–4.04). Conclusion There seems to be a greater prevalence of MHS in active professional male footballers than in the global population and other sports. After severe injury/surgery, the risk of developing MHS is increased, confirming that healthcare professionals should be aware of the mental health of injured players.

Coconut trees are vital in various agricultural and economic sectors. Their susceptibility to nutrient deficiencies poses a significant threat to growth and productivity. Traditional nutrient assessment methods are time and labour-intensive, relying on manual inspection and chemical testing. There has been no significant research on detecting nutrient deficiencies in coconut trees. Additionally, assessing deficiency, and severity automatically and recommending suitable fertilizers remain unexplored. This research leverages the YOLOv9 model to identify macro and micronutrient deficiencies in coconut trees and proposes an Image Analysis based Severity Detection (IASD), to determine the severity of these deficiencies. Along with these a Severity Index Calculation Model (SICM) is also introduced that calculates the Severity Index (SI) of these deficiencies. For each identified deficiency, the appropriate fertilizer and its application quantity are suggested. Four deep learning models—RetinaNet, Faster Regional Convolutional Neural Network (Faster R-CNN), You Only Look Once version 5 (YOLOv5), and version 9 (YOLOv9) —were compared for the prediction of nutrient deficiencies in coconut trees using a dataset of 5,720 images of nutrient-deficient coconut tree leaves. YOLOv9 outperformed other models with Accuracy, Precision, and Recall values of 80 %, 98.59 %, and 80.37 %, respectively. Manual verification ensured the correctness of IASD and SICM predictions during model creation, providing farmers and agricultural professionals with a precise, automated tool for managing coconut plantations.

Secondary lymphedema is a common complication following surgical treatment of solid tumors. Although more prevalent in women due to higher breast cancer rates, men also develop lymphedema, often with more severe manifestations. Despite these differences in clinical presentation, the cellular mechanisms underlying sex differences are poorly understood. Previous studies have shown that inducible nitric oxide synthase (iNOS) expression by inflammatory cells is an important regulator of lymphatic pumping and leakiness in lymphedema and that lymphatic endothelial cells are highly sensitive to nitrosative stress. Based on this rationale, we used a mouse tail model of lymphedema to study the role of nitric oxide in sex-related differences in disease severity. Consistent with clinical findings, we found that male mice have significantly worse tail edema and higher rates of tail necrosis compared with female mice following tail skin/lymphatic excision (p = 0.001). Our findings correlated with increased tissue infiltration of iNOS + inflammatory cells, increased iNOS protein expression, and increased nitrosative stress in male mouse lymphedematous skin tissues (p < 0.05). Importantly, transgenic male mice lacking the iNOS gene (iNOS-KO) displayed markedly reduced swelling, inflammation, and tissue necrosis rates, whereas no differences were observed between wild-type and iNOS-KO female mice. Overall, our results indicate that iNOS-mediated nitric oxide production contributes to sex-based differences in secondary lymphedema severity, emphasizing the need to consider sex as a biological variable in lymphedema research.

Contemporary patient care requires a multidisciplinary approach to monitoring, assessing, and managing diseases. Promoting multidisciplinary approaches encourages the purposeful participation of many healthcare professionals and harnessing their combined knowledge to provide tailored treatment plans. Pharmacists, skilled and knowledgeable professionals in medication management, drug-related problems, and disease prevention, can offer vital interventions that contribute to improved patient outcomes. Advances in healthcare and information technology have expanded pharmacists' professional roles and made them essential in healthcare. Pharmacist-managed clinics (PMCs), an innovative healthcare approach, could potentially improve patient safety, satisfaction, accessibility, and affordability to quality healthcare. Spread across the healthcare continuum, pharmacists have a well-defined role in providing comprehensive pharmaceutical care and interprofessional collaboration, further reinforcing the necessity of establishing PMCs. This narrative review aims to compile and summarize information on PMCs from PubMed, Scopus, Web of Science, and Google Scholar till December 2023. The PMC shortlist covers specialties such as cardiovascular, hematologic, endocrine, pain medicine, respiratory medicine, infectious diseases, gastrointestinal, nephrology, neurology, and oncology. Pharmacists in disease-specific PMCs have demonstrated improved treatment outcomes and access to specialty care. Additionally, based on peer-reviewed literature, the review also highlights how PMCs enhance the pharmacist's role in improving disease-specific outcomes, overall quality of care, and medication management. The inclusion criteria are randomized controlled trials, case-control studies, cohort studies, and pre-post studies involving patients from cardiology, hematology, endocrinology, pain medicine, respiratory medicine, infectious diseases, neurology, nephrology, gastroenterology, and oncology specialties, focusing on pharmacist-driven clinics, published in English, and covering any geographical location. The exclusion criteria include review articles, proposed models, commentaries, editorials, and those published in languages other than English. Our findings reveal that PMCs are underutilized globally. PMCs work better in developed countries, possibly on account of robust healthcare infrastructure, adequate healthcare budgets, availability of trained pharmacists, and supportive regulatory environments. The review found that pharmacist-led interventions, such as medication monitoring and patient education, significantly enhance therapeutic outcomes. Pharmacist Managed Clinics improve affordability and acceptability, expanding healthcare access in outpatient and inpatient settings. This review also highlights the critical need for implementing PMCs to improve healthcare delivery, particularly in providing comprehensive and accessible services in developing countries.

As a tropical fruit, the banana is one of the favorite fruits, and the banana herb only bears fruit once in its lifespan; it is chopped off after harvesting, and only 40% of it is adequately utilized, leaving the remaining 60% as trash rich in cellulose, hemicellulose, and natural fibers. The pseudostem accounts for 75% of the waste, with the rest accounting for 25%. The purpose of this paper is to raise important awareness about the bioactive chemicals found in banana pseudostems hence they can be considered as sustainable foods and their applications as culinary raw materials and in other sectors. Even though bulk waste is biodegradable, it causes many problems in our environment, so sustainable waste usage with proper management and utilization of the residues can not only help in the utilization of the bioactive and nutrient compounds present but also increase the economic and environmental issues associated with the problem. The banana pseudostem is used in various businesses as culinary ingredients, fibers, and hazardous removers. Even when used for heavy metal treatment in wastewater, the fiber in it is strong enough to manufacture cardboard and food packaging materials. Some pseudostem types can even be extracted for pectin and used as food additives. The powdered pseudostem is used to boost the nutritional content of numerous recipes. In summary, the banana pseudostem can be divided into two first the central core part that can be used as food raw material and other outer sheaths in various different industries.

Background: Catatonia has been reported with withdrawal of medications. Among the various psychotropics, clozapine has been implicated to cause catatonia when abruptly withdrawn. The data regarding clozapine withdrawal catatonia are scarce and are mostly available in the form of case reports and series. Aim: In this case series, we present three cases of clozapine withdrawal catatonia and review the available literature on clozapine withdrawal clozapine. Results: All the three patients developed catatonia within 48 h to 14 days of stoppage of clozapine in the doses of 100–350 mg/day. Two of the patients experiencing clozapine withdrawal improved with reinstitution of clozapine in the previous doses along with benzodiazepines. However, one patient additionally required electroconvulsive therapy (ECT) for the management of withdrawal catatonia. A review of literature showed that clozapine withdrawal catatonia has male preponderance and has been reported with withdrawal of clozapine doses of 50–550 mg/day. The catatonia usually starts in 48 h to 2 weeks of stopping clozapine. Available literature suggests that most of the patients have been managed with restarting of clozapine, along with lorazepam and occasional patients require ECT. Conclusions: To conclude our case series and the available review of literature suggests that sudden discontinuation of clozapine can lead to withdrawal catatonia. Our cases also highlight the importance of reviewing treatment history, especially of abrupt discontinuation of clozapine when a patient on clozapine present with catatonia. This can be very helpful in deciding about further management.

Human serum albumin (HSA) is synthesized by the liver, accounting for 60 % of total plasma protein in vertebrates' blood. It is the most predominant extracellular plasma protein that acts as a repository and transporter of exogenous and endogenous substances in the blood of healthy humans. Decreased albumin concentration in the human body or its abnormal levels indicate the occurrence of hepatic, renal, and digestive-related diseases. Therefore, accurate quantification of HSA is of great significance in diagnostic testing and routine clinical analysis of albumin-linked diseases. Herein, a class of triphenylamine rhodanine-3-acetic acid (mRA)-a bifunctional fluorescent molecule with twisted intramolecular charge transfer (TICT)-induced emission characteristics is synthesized and employed as a novel sensing probe for the fluorescent detection of human albumin. mRA can be selectively lighted up through site-specific interactions with serum albumin-binding moieties and show enhanced photophysical or biological response efficacy. Understanding the interaction of mRA with HSA at the molecular level was carried out using docking methodology to explore the site-specific interaction phenomenon. The resulting fluorescence strategy produced a dose-dependent signal response enhancement upon interaction with HSA in the concentration range of 0.01–400 μg/ml. The sensor probe exhibits a low detection limit of 10 ng/mL and is found to be a feasible, low-cost, and effective approach for HSA analysis in complex biological fluids for early detection and diagnosis of albumin-related diseases.

Introduction: Family planning includes a wide range of services, such as counseling, contraception, and support to couples. Evidence shows that developing countries have a high degree of inequality in contraception use and prevalence. Reasons for these inequalities include cultural barriers such as traditional preferences and a desire for larger families and lineage, especially in rural areas. The primary objective of this research was to examine the updated contraceptive method preferences of couples in rural and urban regions of Pakistan and how these translate to family planning practices among the different provinces. Methods: A secondary survey analysis using the Pakistan Demographic and Health Survey 2019 survey data was conducted. The dataset included 15 143 women sampled proportionally from the provinces, including Gilgit Baltistan and Azad Jammu and Kashmir. The unit of analysis was 'women' from the individual survey dataset. Age, type of residence (rural, urban), division, education level, and language were used to evaluate access to family planning and contraception services. The c2 test assessed the relationship between dependent and independent variables. Multivariate logistic regression analysis was then performed to see the likelihood of contraceptive use among women. Results: Of the women in the sample, 55% were from rural areas and 50% were without formal education; 51.7% of these women were using or practicing any form of contraception method. The most common method used was condoms (9.2%), followed by injectables (6.2%). Regression analysis showed that women aged 15-19 years were less likely (odds ratio (OR)=0.71, 95% confidence interval (CI)=0.51-1.01) to use contraception when compared to the reference group. The likelihood of contraceptive use was higher in urban areas (OR=1.53, 95%Cl=1.39-1.69). Noticeably, contraceptive use was less likely in uneducated women (OR=0.62, 95%Cl=0.56-070). Punjab province had the highest contraceptive prevalence (34.3%), whereas Baluchistan had the lowest (6.9%). The use of contraception in urban and rural populations was similar in all provinces except Sindh and Gilgit Baltistan. In urban and rural areas, women in the age group 30-35 years who use contraception show a prevalence of 21% and 22% respectively. Conclusion: The study highlights suboptimal use of contraceptives and the existence of high levels of inequalities among the regions. There is a need for the implementation of focused educational initiatives and counseling interventions along with prioritization of accessibility and affordability of contraceptive methods among women in lower socioeconomic regions.

Abstract Background Non‐small cell lung cancer (NSCLC) is often diagnosed at an advanced stage. Clinical trials have demonstrated that first‐line immunotherapy alone or in combination with chemotherapy improves overall survival. However, reports of survival outcomes in real‐world settings are limited. We assessed survival in advanced NSCLC patients treated with immunotherapy alone or in combination with chemotherapy in first‐ or second‐line at the Windsor Regional Cancer Program (WRCP) and compared it to existing literature. Methods We included patients diagnosed with stage IV NSCLC from January 2015 to December 2020 and treated with first‐line chemoimmunotherapy (ChemoImmuno1), chemotherapy followed by immunotherapy (Chemo1), or immunotherapy followed by chemotherapy (Immno1) in our survival analysis. Patients with oncogene‐addicted mutations were excluded. Results There were 160 patients of which 41.5% were female. Mean age was 68 years. Median overall survival from time of diagnosis was 474 days (95% CI: 249, 949) with an estimated 5‐year survival of 11.1% (95% CI: 4.5, 21.3). Median OS in ChemoImmuno1 was 9.6 months, in Chemo1 was 19.2 months from time of diagnosis and 10.5 months from time of initiation of immunotherapy, and in Immuno1 was 18.4 months, respectively. Estimated survival at three years from time of diagnosis for ChemoImmuno1 was 17.6% and for Immuno1 was 17.9%. For Chemo1, from diagnosis it was 20.1% and from second‐line therapy it was 15.4%. Survival outcomes were comparable to clinical trials and other studies. Conclusion Real‐world survival outcomes of immunotherapy for advanced NSCLC are comparable to the existing literature in this single center study.

In this paper, the feasibility of employing a fast intensity modulator and a chirped fibre Bragg grating to build a dispersion-tuned mode-locked swept laser (DTML-SS) for OCT at 850 nm is evaluated. Stable mode-locking is achieved by applying 50 ps pulses at 1 GHz to the modulator, obtaining 35 nm tuning range at 10 KHz sweep rate and 1 mm axial range.

A detailed description of the Limbus and the pupil.Anatomy of limbus which is a cardinal structure in ocular surgeries and its importance in maintaining corneal integrity is discussed .Also provided is a clinical algorithm on assessment of pupil.

Abstract Despite significant research, mechanisms underlying the failure of islet beta cells that result in type 2 diabetes (T2D) are still under investigation. Here, we report that Sox9, a transcriptional regulator of pancreas development, also functions in mature beta cells. Our results show that Sox9-depleted rodent beta cells have defective insulin secretion, and aging animals develop glucose intolerance, mimicking the progressive degeneration observed in T2D. Using genome editing in human stem cells, we show that beta cells lacking SOX9 have stunted first-phase insulin secretion. In human and rodent cells, loss of Sox9 disrupts alternative splicing and triggers accumulation of non-functional isoforms of genes with key roles in beta cell function. Sox9 depletion reduces expression of protein-coding splice variants of the serine-rich splicing factor arginine SRSF5, a major splicing enhancer that regulates alternative splicing. Our data highlight the role of SOX9 as a regulator of alternative splicing in mature beta cell function.

Sensory parameters of food and beverages secured much importance with mounting changes in diet preferences. The taste and flavour of these food groups are largely affected by astringency which in turn influence the sensory experience. Derived from Latin, astringency deals with the sensation of extreme dryness, roughness or puckering involving the secretions of salivary glands. Accountable astringent clusters majorly revolving around the presence of tannins in food trailed by the incidence of salts of multivalent cations like Al, Zn, Cr etc, and dehydrating agents like mineral acids, alcohol etc. To augment the sensory feeling and to broaden the marketing possibilities related to beverages, it is important to accomplish techniques to reduce or control the development of these sensations. De-astringency practices performed in foods can be broadly catalogued into thermal and non-thermal treatments. While the former majorly included hot water, steam and microwave treatment, the latter concentrated mainly on innovative techniques like high hydrostatic pressure, pulsed electric field, thermo-sonication, ultrasonication etc. The effectiveness of these procedures is largely dependent on the mechanisms associated with the development of astringency feelings in foods. Understanding the mechanism underlying astringency sensation is still in a nascent stage and needs more exploration to state the explicit reason behind the process. This review chiefly covers the explanation of astringency, the mechanism involved and the different de-astringency techniques as per the prevailing astringency models.

There is scarce research on the use of black soldier fly larvae (BSFL) meal in the diets of Atlantic salmon (Salmo salar) reared in a real farm. Thus, the current study aimed to evaluate the effect of feeding BSFL meal to salmon in terms of fillet quality and sensory analysis at farm level conditions. Fish were fed a total of three diets (pellet size; 4.5 mm and 9 mm); a control diet (Control: 0% BSFL meal) formulated using a standard commercial recipe, containing FM (10%) and plant-based proteins. Two experimental diets were formulated using partially defatted BSFL meal (53% crude protein), in which plant-based proteins at 37% inclusion level from the control diet (4.5 mm pellets) were replaced by BSFL meal at 5% (BSFL 5%) and 10% (BSFL 10%). While for the 9.0 mm pellets, plant-based proteins at 35% were replaced by the BSFL meal at 5% and 10% inclusion level. All the three diets were formulated to be iso-nitrogenous and iso-lipidic diets. Diets were assigned to the sea-cages in triplicate (12 ×12 m; 1900 m3); housing ∼6000 fish per cage from 173 ± 2.7 g to harvest size 3.45 ± 74 kg for a period of 13 months. Feeding salmon with BSFL meal had no negative impact on the general fillet physical, chemical, and nutritional parameters. The fillet sensory evaluation showed that the control samples were associated with the typical attributes such as fresh salmon taste, while BSLF 10% to rancid taste but these attributes were not significantly different among the dietary groups. Overall, the current study showed that diet containing BSFL meal did not affect the general fillet parameters compared to salmon fed a commercial diet and thus validate the inclusion of BSFL meal up to 10% reared for a whole production cycle in sea cages.

Abstract Civets are frugivorous animals in the Order Carnivora. They are relatively less shy towards people and anthropogenic habitats. It has been reported that the civets’ preference of defecating in open sites enable them to be important seed dispersers of degraded forests and urban ecosystems of Asia and Africa. We surveyed for scats of palm civet (Paradoxurus hermaphroditus) in forest fragments of sacred groves (closed), coffee plantations (partly closed) and home gardens (relatively open) during the fruit ripening period of Coffee and Caryota urens – the two preferred fruits of civet – to report the microhabitat characteristics and seed composition of civet latrines. The microhabitat of each scat position – whether on or off the ground and the shade type – was recorded. The scat analysis showed the presence of 4234 seeds belonging to coffee (90.2%), C. urens (9.7%), and an anonymous Rubiacea species (0.10%) in a total of 105 scats collected from coffee plantations (55), home gardens (5), and sacred groves (45). The number of scats sampled from the three habitats was different, but not the number of seeds per scat. Overall, the number of scats increased with the canopy cover, but the trend was different for different habitats. In home garden and coffee plantations, it decreased, but in sacred groves, it increased with the canopy cover. The number of scats sampled above the ground – on tree branches, logs and built-up structures– was more than that was on the ground. The findings contradict the general belief that the civet latrines occur more in open areas than the shaded areas. Because the civet latrines are seen more above ground than on the ground, their efficiency as seed dispersal agent may be examined critically in different contexts.

BACKGROUND: Online dating is becoming more and more popular not only among the adult population, but also among adolescents, which comes with its own advantages and disadvantages. Adolescents are more vulnerable to a number of issues connected with online dating, including online grooming, bullying, emotional abuse, revenge porn, harassment, and lack of social interaction. AIM: We aimed to briefly review the available literature exploring the impact of online dating on adolescents, with special reference to the current Indian Scenario. METHODS: A brief literature search was conducted in PubMed and Google Scholar in September 2022 with no date limits. Keywords included various combinations of terms such as online dating, dating applications, social media, mental illness, psychiatric disorders, adolescents, and mental health. Original studies and review articles exploring the impact of online dating on adolescents and published in English were reviewed in our work. A descriptive strategy was used to summarise the findings. RESULTS: The impact of online dating on adolescents is discussed in the light of (1) issues associated with online dating among adolescents, (2) the international context, and (3) Indian context. CONCLUSION: Since the beginning of the COVID-19 pandemic, online dating has grown in popularity among adolescents, which has led to a number of worrying situations, including increased risk of sexually transmitted infections, dating violence, and mental health issues. All of these issues are described in the literature in the context of unsupervised use of technology, peer pressure, and desire to fit into the society. Data from India remain scarce on this topic, highlighting the need for research exploring the influence of online dating on adolescents.

Abstract Graphene is emerging in numerous wearable and biomedical applications. Understanding the impact of graphene‐based nanocomposites over bacterial activity is critical for design appropriate technical solutions. In this work, antibacterial activity of monolayer and few‐layer graphene is tested against Gram‐negative and Gram‐positive model bacteria. Such graphene‐based nanomaterials present different effects on bacteria viability, depending on concentration and shape. The presence of dopant agents can either increase or revert graphene antibacterial effect. Among other bactericidal mechanisms, we also demonstrate that graphene surface polarity is connected to antibacterial properties and can be easily modified by surface charge transfer doping. This electrophilicity tuning offers a novel mechanism for the molecular basis of graphene's antibacterial activity.

Background: The prevalence of diabetes and its adverse effects have snowballed through the world due to changes in lifestyle. Association of diabetes with hearing loss has been a topic of discussion for many years. Aim and Objectives: To assess the Sensorineural Hearing Loss (SNHL) in patients with diabetes and correlate it with the duration and severity of hyperglycemia. Material and Methods: This hospital based cross-sectional study was carried out from August 2017 to July 2018 among 100 patients with diabetes aged between 31-60 years. Patients were grouped as per age, duration of Diabetes Mellitus (DM) and glycaemic control using HbA1c levels. The results were statistically analysed using Chi-square test and Pearson's coefficient to draw significance. Results: The incidence of SNHL in patients with diabetes was 79% and the correlation among different age group showed statistical significance (p < 0.05). There was no statistical association between the gender and hearing loss among patients with diabetes (p = 0.70). However, association of SNHL to the duration of DM was statistically significant when compared among all three duration groups. No statistical correlation was present with the glycaemic control of DM. Results showed high frequency SNHL with 41.8% patients with diabetes having moderate degree of hearing loss. Conclusion: Early referral for hearing assessment in patients with diabetes can be followed by clinicians aiming at improving quality of life with timely measures.

Abstract Early postoperative mortality risk prediction is crucial for clinical management of gastric cancer. This study aims to predict 90-day mortality in gastric cancer patients undergoing gastrectomy using automated machine learning (AutoML), optimize models for preoperative prediction, and identify factors influential in prediction. National Cancer Database was used to identify stage I–III gastric cancer patients undergoing gastrectomy between 2004 and 2016. 26 features were used to train predictive models using H2O.ai AutoML. Performance on validation cohort was measured. In 39,108 patients, 90-day mortality rate was 8.8%. The highest performing model was an ensemble (AUC = 0.77); older age, nodal ratio, and length of inpatient stay (LOS) following surgery were most influential for prediction. Removing the latter two parameters decreased model performance (AUC 0.71). For optimizing models for preoperative use, models were developed to first predict node ratio or LOS, and these predicted values were inputted for 90-day mortality prediction (AUC of 0.73–0.74). AutoML performed well in predicting 90-day mortality in a larger cohort of gastric cancer patients that underwent gastrectomy. These models can be implemented preoperatively to inform prognostication and patient selection for surgery. Our study supports broader evaluation and application of AutoML to guide surgical oncologic care.

Key message Hydatid disease is a zoonotic parasitic infestation caused by tapeworms, particularly Echinococcus granulosus. The lung is the second most site followed by liver accounting for 10%–30% of the cases. Rupture of hydatid cyst can occur into pleura or bronchus. Surgery remains the mainstay of treatment for pulmonary hydatid.

Abstract Background Depression is the leading cause of global disability and can develop following the change in body image and functional capacity associated with stoma surgery. However, reported prevalence across the literature is unknown. Accordingly, we performed a systematic review and meta-analysis aiming to characterise depressive symptoms after stoma surgery and potential predictive factors. Methods PubMed/MEDLINE, Embase, CINAHL and Cochrane Library were searched from respective database inception to 6 March 2023 for studies reporting rates of depressive symptoms after stoma surgery. Risk of bias was assessed using the Downs and Black checklist for non-randomised studies of interventions (NRSIs), and Cochrane RoB2 tool for randomised controlled trials (RCTs). Meta-analysis incorporated meta-regressions and a random-effects model. Registration: PROSPERO, CRD42021262345. Results From 5,742 records, 68 studies were included. According to Downs and Black checklist, the 65 NRSIs were of low to moderate methodological quality. According to Cochrane RoB2, the three RCTs ranged from low risk of bias to some concerns of bias. Thirty-eight studies reported rates of depressive symptoms after stoma surgery as a proportion of the respective study populations, and from these, the median rate across all timepoints was 42.9% 42.9% (IQR: 24.2–58.9%). Pooled scores for respective validated depression measures (Hospital Anxiety and Depression Score (HADS), Beck Depression Inventory (BDI), and Patient Health Questionnaire-9 (PHQ-9)) across studies reporting those scores were below clinical thresholds for major depressive disorder according to severity criteria of the respective scores. In the three studies that used the HADS to compare non-stoma versus stoma surgical populations, depressive symptoms were 58% less frequent in non-stoma populations. Region (Asia–Pacific; Europe; Middle East/Africa; North America) was significantly associated with postoperative depressive symptoms (p = 0.002), whereas age (p = 0.592) and sex (p = 0.069) were not. Conclusions Depressive symptoms occur in almost half of stoma surgery patients, which is higher than the general population, and many inflammatory bowel disease and colorectal cancer populations outlined in the literature. However, validated measures suggest this is mostly at a level of clinical severity below major depressive disorder. Stoma patient outcomes and postoperative psychosocial adjustment may be enhanced by increased psychological evaluation and care in the perioperative period.

ABSTRACTSesame is an indeterminate oilseed crop where yield is determined by source and its capacity for synthesis and mobilization of photosynthates. Knowledge of the existing relation between source and sink and its validation under deficit soil moisture conditions is limited in sesame. Therefore, experiments were conducted using 25 sesame genotypes with differing plant architecture in terms of branching pattern (mono to multiple) and leaf area (minimum to maximum) under both irrigated (IR) and deficit soil moisture stress (DS) conditions for two consecutive years 2021 and 2022. The variations existing among the source and sink traits were measured, and the ANOVA of the measured traits revealed a significant differences among the genotypes for source traits, viz. leaf area, stem and leaf dry weight, net photosynthesis, total dry matter and their relationship with sink, i.e. seed yield under IR and DS conditions, and it was further confirmed using regression analysis. The results showed, certain source traits viz., stem dry weight and total dry matter at seed filling stage were positively associated with seed yield at both IR and DS conditions. Additionally, cluster analysis showed that the genotypes IC 54039-B-1 and SI 1802 had a proportional leaf area with good seed yield and displayed a better source and sink relationship, highlighting the fact that photosynthates were efficiently translocated to the sink. The genotypes IC 54039-B-1 and SI 1802 outperformed IR under DS with minimal yield differences and optimal leaf weight, total dry matter, and plant stem weight. These genotypes with better source and sink relationships can be used in sesame crop improvement programmes.

Objective: To determine the neurocognitive function of active professional male footballers, determine whether deficits/impairments exist, and investigate the association between previous concussion(s) and neurocognitive function. Methods: An observational cross-sectional study conducted via electronic questionnaires. The CNS Vital Signs online testing system was used to evaluate neurocognitive function. Results: Of the 101 participants, 91 completed the neurocognitive function testing. Neurocognitive function domain deficits or impairments were unlikely in 54.5–89.1%, slight in 5.9–21.8%, moderate in 1.0–9.9%, and likely in 4.0–14.9% of participants. A history of zero concussions found a significant association between the neurocognitive index (Odds Ratio [OR] 0.6; 95% CI 0.2–0.4) and complex attention domain (OR 0.3; 95% CI 0.1–0.9), with 40% and 70% less odds, respectively, of deficit/impairment. Among the 54.5% who reported any number of concussions, there were increased odds of neurocognitive domain deficits/impairments for complex attention (CA) [3.4 times more] and simple attention (SA) [3.1 times more]. Conclusion: In the active professional male footballer, most neurocognitive functions do not have significant deficits/impairments. The odds of neurocognitive function deficit/impairment were significantly increased threefold for CA and SA in those who reported a history of any concussion(s).

Background: Gender preference among women remains a significant phenomenon that reflects the imbalance in the sex ratio. Understanding the determinants of gender preference is crucial in addressing associated challenges and promoting gender equality. Objectives: To study the socio-demographic profile of post-natal women, gender preference among them and the factors affecting their preference. Methodology: A hospital-based Cross-Sectional Study was conducted in a tertiary hospital in Mangalore among 155 multiparous postnatal women. The data were collected using a pre-tested semi-structured questionnaire and was analysed using SPSS version 17.0. Results: Overall, 54.83 % of the study participants belonged to the age group of 18-27 years, 57.42% were unaware of any techniques for determining a person’s sex, and the most frequently mentioned method was ultrasound. The proportion of respondents with a preference for a male child was only 20.6%. The most common reason was the male gender carrying the family name. According to 73% of respondents, having two children is ideal for a family, while having an equal number of boys and girls was the most desired combination (60%). There is a significant preference (75%) for the second child to be male if the first child was female. Conclusion: It’s noteworthy that the preference for a male child was often tied to cultural and familial considerations, such as carrying on the family name. Interestingly, the study also highlights a prevalent preference for a second child to be male if the first child was female, signifying the persistence of gender-biased attitudes.

This study shows the effect of the ultrasound-assisted extraction (UAE) method in desi chickpea protein isolate at 50 % and 100 % amplitude i.e., 200 W – 24 kHz at various processing times. The changes occur to the functional properties of desi chickpea protein compared to the conventional extraction method. The UAE approach yielded more protein than the conventional method. The solubility also increased in the UAE method at 15 min at 200 W. The highest solubility was 91.1 %, and the lowest was in the conventional extraction method, 85.5 %. In emulsion, oil holding capacity and stability, foaming capacity, and stability were also increased according to the increase in the ultrasound than the conventional extraction method. The water-holding capacity was decreased in the UAE method. The WHC in the US-assisted extracted protein was 1.528 g, and in conventional method, 1.614 g. The digestibility in US-treated protein shows better results than the control. In FT-IR, there was a variation in the nitro and cyclic alkane compounds in the US-treated sample. According to the results from SEM, zeta, particle size, and SDS PAGE, desi chickpea protein isolate powder showed smooth surface, decrease in zeta potential along with the particle size and legumin, vicilin protein fractions are detected respectively.

Novel visual intelligent pH-indicator film was prepared from the eggshell membrane- gelatin, pectin, and anthocyanin pigment from butterfly pea flower (BP) (Clitoria ternatea). It was used as a real-time pH indicator for predicting food freshness. Eggshells are useful biowaste, gelatin has been extracted from the eggshell membrane and was used for fabricating the film. The Anthocyanin content of BP extract was 198.3 mg g−1. The film's surface morphology and chemical nature were estimated using a Scanning electron microscope, Fourier transform infrared spectroscopy, and X-ray diffraction technique. The film was pH responsive and exhibited color variation ranging from shades of red, purple, blue, green, and yellow at different pH (1-13). The applicability of the developed pH- indicator film was studied on fresh Tilapia fish by monitoring its deterioration for a specific time period. The film showed a visible color change after seven days of storage at 4ºC from dark blue, bluish-grey to olive, and deep green. Change in the total volatile basic nitrogen (TVB-N) content and pH change had an effect on the color response of the film. The findings demonstrated that the fabricated pH indicator film proved to be pH sensitive and could be used to monitor fish freshness.

Atlantic salmon (Salmo salar) were fed diets containing black soldier fly larvae (BSFL) meal at two inclusion levels for 13 months in open sea-cages. BSFL meal replaced plant-based ingredients and dietary insect meal inclusion levels were at 5% and 10%. A commercial salmon diet was fed as a control diet. Fish were reared from ~173 g to ~ 4 kg and were randomly distributed into nine open sea-cages with ~6000 salmon/cage (12×12 m2; 1900 m3). Fish from the sea-cages were sampled at two time points (mid and final samplings) to study the dietary effects of BSFL meal on the general health and welfare. Monthly assessments of sea-lice and gill score were conducted to evaluate the overall well-being of the salmon. The findings from the current study revealed that dietary inclusion of BSFL meal up to 10% did not have any significant effects in general growth, welfare or survival. However, significant positive response was observed in the general skin mucosal, hematological, and gene expression profiles of salmon. Notably, the group of salmon fed with 5% BSFL meal showed a significant decrease in plasma aspartate transaminase and alanine transaminase. Also, a significantly higher expression of interleukin1β in both skin and gill along with upregulation of matrix metallopeptidase9 and mucin18 in gill were observed in salmon fed BSFL at 5%, which aided in increased immune responses. Apart from that, this group had significantly higher mucus secretions, decreased cortisol response and increased number of erythrocytes. Furthermore, the delousing stress had a significant effect on the plasma cortisol, and these responses were independent of the dietary effect. Moreover, these immune responses behaved differently at different fish size and time points, acknowledging the influence of various factors in immune modulation. Overall, the findings from this study showed the effects of dietary BSFL meal to modulate the immune status of salmon. This study aims to fill the existing knowledge gaps regarding the impact of incorporating BSFL meal as a functional feed ingredient into the salmon diet on health and immune status replicating real farm conditions.

Background: Hindlimb-unloaded (HU) mice are animal models of simulated microgravity that exhibit pathological changes in several organs; however, relevant findings regarding the kidneys remain elusive. We investigated the possible contribution of protein dysregulation through endoplasmic reticulum (ER) stress to renal pathology in HU mice. Methods: We categorized male C57BL/6j mice into ground-based control and HU groups and treated them daily with a placebo or 4-phenylbutyrate (4PBA, an ER stress inhibitor), respectively, for 3 weeks. HU mice showed reduced body weight, whereas kidney weight remained unchanged. Results: Treatment with 4PBA increased the kidney weight of HU mice. The histopathological changes in HU mouse kidneys, including the widening of the Bowman's capsule and increased glomerular area, were reversed through 4PBA treatment, accompanied by a 4PBA-induced reduction in the expression of several ER stress markers. Particularly, we found a reduction in the splicing of the X-box binding protein, indicating reduced ER stress in 4PBA-treated HU mice. Thus, 4PBA may use additional mechanisms to suppress ER stress and prevent renal pathology in HU mice. Conclusion: Our findings are the first to suggest that HU causes disruption of renal microarchitecture and that 4PBA may be a potent drug for kidney restoration under HU. Our preliminary findings have translational potential for conditions that mimic HU, such as prolonged bed rest.

The proliferation of electromagnetic (EM) pollution is more rapid now and can be more outrageous in near future due to the exponential growth of the electronics industry. Strategy of using natural fibers for fabricating efficient EMI shielding materials is an environmentally benign solution for this serious peril. With this motivation, we are introducing novel e-textile derived from sustainable jute fabrics that are capable of suppressing and absorbing EM radiations in the frequency range 8.2–12.4 GHz (X-band). In the present work, jute fabrics were in-situ polymerized with pyrrole monomer. These conductive fabrics were then characterized and correlated for their structure, morphology, thermal stability, electrical conductivity, and EMI shielding capabilities. Moreover, this e-textile showcased excellent electrical conductivity of 1.160 S/cm and exhibited a maximum EMI shielding effectiveness value of −30.2 dB (>99.9% blockage) at 1 mm fabric thickness by a predominant absorption behavior with menial reflection or secondary pollution, which is highly beneficial for commercial applications. This is so far the first report of jute fabrics as e-textile with the highest shielding efficacy value reported, which makes them an ideal candidate for next generation of smart and wearable electronic textiles for futuristic applications such as robotics and artificial intelligence (AI).

Abstract Pollinator conservation is a global priority. Efforts are taken to restore pollinators by improving flower resources, a crucial driver of pollinator diversity and population growth. It helped gardening and landscaping supply chains, which introduced lists of bee-friendly plants and bee hotels, yet, desirable results seem distant. One shortcoming of the present schemes is that they lack a cohesive planning for nesting opportunities and nesting provisions for wild solitary bees, the crucial pollinators of crop and wild plants. We tested whether the world’s popular ornamental plant, rose (Rosa chinensis Jacq.)—a hitherto unlisted bee-friendly plant—can aid in conserving leafcutter bees, which require fresh leaves for constructing nest cells. We surveyed 2360 rose plants in 136 sites in rural and urban places and lowlands and highlands of south (8°N–12°N) and northeastern India (26°N–27°N) for the characteristic notches the bees leave on foraged leaves. We reared brood constructed with rose and non-rose leaves to examine the brood success rate. About a quarter of all the roses surveyed had the notches of leafcutter bees on the leaves. However, the proportion of cut roses varied considerably among sites. Bees used roses much higher in urban areas and lowlands than in rural areas and highlands. The selection of plants was negatively associated with pesticide application. The brood success rate was 100% for the brood that was constructed by the leaves of rose and non-rose plants. Rose flowers do not support bees, but rose leaves indeed do. We recommend rose plants in leafcutter bee conservation and restoration schemes, particularly in urban environment.

Abstract Mechanical unloading of the body in the hindlimb unloaded (HU) mice induces pathology in multiple organs, but the effects on testes are poorly characterized. We investigated the histology and Raman spectroscopy of the mouse testes following HU condition. We divided male, c57BL/6j mice into ground-based controls or experimental groups for two and four weeks of HU. The testes tissues were dissected after euthanasia to investigate histological and Raman spectroscopic analysis. We found an HU-induced atrophy of testes irrespective of the time duration (p

Lei et al. systematically characterized the epistasis among natural mutations in the neuraminidase of human influenza H3N2 virus, which provide insights into the biophysical constraints that shaped its evolution trajectory over the past half-century.

Leadership training is a necessary component of undergraduate medical education. Our group successfully implemented a student-led organization starting from 2016 (Student Leadership Development Initiative; SLDI) that aimed to provide medical students with exposure to physician-leader career paths in an informal, organic, interactive setting. The COVID-19 pandemic necessitated a shift to online programming, and given the high prevalence of ZOOM TM fatigue, we incorporated monthly, freely available, self-directed modules as an additional leadership training opportunity. The goals of this study are to assess the (1) feasibility of and participation in a virtual student organization focused on leadership training, (2) whether students’ perceptions of the importance of leadership were associated with participation in SLDI, and (3) lessons learned from transitioning to virtual modalities. An anonymous, retrospective cross-sectional survey with 13-items was distributed through an email listserv and a 6-question survey was sent to attendees following each virtual group-discussion. A Fisher's exact test was conducted to assess whether the number of modules completed was associated with students’ perception of leadership importance. Survey results showed that 85% strongly agreed or agreed that SLDI helped them develop professional goals and career paths, and 74% reported benefits in becoming more compassionate physician leaders and valuing wellness. All respondents completed ≥1 self-directed module, and the students’ perception of leadership importance did not influence the number of self-directed modules completed ( p > .05). Most participants (63%) attended ≥67% of virtual events, and postevent feedback was positive; however, only 46% of respondents reported meeting someone new at events and 32% reported that they intended on connecting with new contacts. Our results suggest that virtual leadership student-organization, involving small-group discussions and self-directed modules, is feasible and beneficial for medical students. However, the inability to promote meaningful networking opportunities is a major limitation of a virtual training model.

Lung infection with the fungus Aspergillus fumigatus (Af) is a common complication in cystic fibrosis (CF) and is associated with loss of pulmonary function. We established a fungal epithelial co-culture model to examine the impact of Af infection on CF bronchial epithelial barrier function using Af strains 10AF and AF293-GFP, and the CFBE41o- cell line homozygous for the F508del mutation with (CF+CFTR) and without (CF) normal CFTR expression. Following exposure of the epithelial surface to Af conidia, formation of germlings (early stages of fungal growth) was detected after 9-12 hours and hyphae (mature fungal growth) after 12-24 hours. During fungal morphogenesis, bronchial epithelial cells showed signs of damage including rounding, and partial detachment after 24 hours. Fluorescently labeled conidia were internalized after 6 hours and more internalized conidia were observed in CF compared to CF+CFTR cells. Infection of the apical surface with 10AF conidia, germlings, or hyphae was performed to determine growth stage-specific effects on tight junction protein zona occludens protein 1 (ZO-1) expression and transepithelial electrical resistance (TER). In response to infection with conidia or germlings, epithelial barrier function degraded time-dependently (based on ZO-1 immunofluorescence and TER) with a delayed onset in CF+CFTR cell monolayers and required viable fungi and apical application. Infection with hyphae caused an earlier onset and faster rate of decline in TER compared to conidia and germlings. Gliotoxin, a major Af virulence factor, caused a rapid decline in TER and induced a transient chloride secretory response in CF+CFTR but not CF cells. Our findings suggest growth and internalization of Af result in deleterious effects on bronchial epithelial barrier function that occurred more rapidly in the absence of CFTR. Bronchial epithelial barrier breakdown was time-dependent and morphotype-specific and mimicked by acute administration of gliotoxin. Our study also suggests a protective role for CFTR by turning on CFTR-dependent chloride transport in response to gliotoxin, a mechanism that will support mucociliary clearance, and could delay the loss of epithelial integrity during fungal development in vivo.

Background: Python is a popular and easy-to-use programming language. It is constantly expanding, with new features and libraries being introduced daily for a broad range of applications. This dynamic expansion needs a robust support structure for developers to effectively utilise the language. Aim: In this study we conduct an in-depth analysis focusing on several research topics to understand the theme of Python questions and identify the challenges that developers encounter, using the questions posted on Stack Overflow. Method:We perform a quantitative and qualitative analysis of Python questions in Stack Overflow. Topic Modelling is also used to determine the most popular and difficult topics among developers. Results: The findings of this study revealed a recent surge in questions about scientific computing libraries pandas and TensorFlow. Also, we observed that the discussion of Data Structures and Formats is more popular in the Python community, whereas areas such as Installation, Deployment, and IDE are still challenging. Conclusion: This study can direct the research and development community to put more emphasis on tackling the actual issues that Python programmers are facing.

This study reports the feasibility of recycled polyvinylidene difluoride (PVDF) beads to decolourize methylene blue (MB) from aqueous streams. The beads were characterized using scanning electron microscopy (SEM), X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR) for its morphological and structural analysis. The effect of various process parameters such as adsorbent dose, initial concentration, contact time, and pH was studied. The first principle density functional theory (DFT) calculations were performed to investigate the underlying mechanism behind the adsorption process. The MB dye adsorption on recycled PVDF beads followed the pseudo-second-order kinetics and Langmuir isotherm, indicating the adsorption was chemical and monolayer. The maximum adsorption capacity obtained was 27.86 mg g−1. The adsorption energy of MB-PVDF predicted from the DFT study was –64.7 kJ mol−1. The HOMO-LUMO energy gap of PVDF decreased from 9.42 eV to 0.50 eV upon interaction with MB dye due to the mixing of molecular orbitals. The DFT simulations showed that the interaction of the MB dye molecule was from the electronegative N atom of the MB dye molecule, implying that electrostatic interactions occurred between the recycled PVDF beads and the positively charged quaternary ammonium groups in MB dye. The present study demonstrates the potential of recycled PVDF beads for a low-cost dye removal technique from textile wastewater. HIGHLIGHTS Experiments and DFT calculations were performed to evaluate the binding of MB dye to PVDF beads synthesized from used membranes.; The adsorption of MB dye on PVDF beads was monolayer and chemical in nature, with a maximum adsorption capacity of 27.86 mg g−1.; The DFT study predicted the adsorption energy of 64.7 kJ mol−1 for MB-PVDF, indicating the strong interaction between MB dye and PVDF.; The study demonstrated the potential of recycled PVDF beads for a low-cost dye removal technique.;

Abstract Muscle disuse in the hindlimb unloaded (HU) mice causes significant atrophy and weakness. However, the cellular and molecular mechanisms driving disuse-muscle atrophy remain elusive. We investigated the potential contribution of proteins dysregulation by sarcoplasmic reticulum (SR), a condition called SR stress, to muscle loss during HU. Male, c57BL/6j mice were assigned to ground-based controls or HU groups treated with vehicle or 4-phenylbutyrate (4-PBA), a potent inhibitor of SR stress, once a day for three weeks. We report that the 4-PBA reduced the SR stress and partly reversed the muscle atrophy and weakness in the HU mice. Transcriptome analysis revealed that several genes were switched on (n = 3688) or differentially expressed (n = 1184) due to HU. GO, and KEGG term analysis revealed alterations in pathways associated with the assembly of cilia and microtubules, extracellular matrix proteins regulation, calcium homeostasis, and immune modulation during HU. The muscle restoration with 4-PBA partly reversed these changes along with differential and unique expression of several genes. The analysis of genes among the two comparisons (HU-v vs. control and HU-t vs. HU-v.) shows 841 genes were overlapped between the two comparisons and they may be regulated by 4-PBA. Altogether, our findings suggest that the pharmacological suppression of SR stress may be an effective strategy to prevent disuse-induced muscle weakness and atrophy.

Peripheral artery disease is a prevalent illness affecting more than 200 million people worldwide. A commonly used technique to manage the condition has been open endarterectomy. However, in recent times, a shift towards minimally invasive techniques has resulted in endovascular intervention as a popular alternative. This review aims to assess the safety and efficacy of endovascular intervention when compared with endarterectomy. A systematic review of the articles published in PubMed, Ovid, Embase, and Scopus within the last 10 years was conducted. The PRISMA guidelines were adhered to, and the Newcastle-Ottawa and NICE quality assessment scales were used. A meta-analysis of proportions was performed using the RStudio software (RStudio Team (2021). RStudio: Integrated Development Environment for R, PBC, Boston, MA, USA). Twenty-six studies were included, with a total of 7126 patients (endovascular, 2496; endarterectomy, 4630). Technical success was greater for endarterectomy than endovascular intervention with an odds ratio of 0.38; 95% CI [0.27–0.54]. In terms of safety as well endovascular intervention was better than endarterectomy with an odds ratio of 0.22; 95% CI [0.15 to 0.31] for wound infection. Endovascular intervention is a safe and effective procedure; however, it cannot be considered superior to endarterectomy.

Context: Sense of coherence (SOC), as stated, is a global orientation that views life as comprehensible, structural, manageable, and coherent. Pregnancy is considered as a crucial time period where an individual experiences increased stress and apprehension which could indirectly affect the child's Quality of Life. Hence, it is important to assess the SOC during this phase of life. Aims: This study aimed to assess the SOC and knowledge about early childhood caries (ECC) among pregnant women and to correlate SOC, level of education, and oral health status of the pregnant women. Materials and Methods: A questionnaire-based cross-sectional study was conducted on pregnant women. The oral health status of the pregnant women was assessed using ICDAS II index and the education status of the participants was also obtained. Statistical Analysis Used: Chi-square test was used for intergroup comparison. Mann–Whitney U-test and Pearson's correlations were done to compare the variables between urban and rural population. Simple linear regression analysis and binary logistic regression were done to determine if SOC varied with change in other variables. Results: The mean SOC among rural and urban population were 33.64 and 41.01, respectively. It was seen that urban pregnant women had more knowledge regarding ECC and less Decayed Missing Filled Teeth (DMFT) as compared to rural pregnant women. There was an inverse relation of knowledge on ECC and SOC with DMFT among urban and rural pregnant women and a direct correlation between knowledge on ECC and SOC with education. Conclusions: This study concludes that SOC and knowledge on ECC are low among rural pregnant women. Furthermore, the SOC correlates with the level of education and oral health status of pregnant women in urban and rural districts of Bangalore, Karnataka.

Introduction Almost one out of four patients with acute middle cerebral artery occlusion also have ipsilateral internal carotid artery occlusion (ICAO). The interventions for acute stroke due to extracranial ICAO with or without intracranial occlusions are still a challenge. Case series reported early revascularization reduced stroke recurrence and improved outcomes. The benefits of this intervention on hyperacute ischemic strokes (within 6 hours) were much less known. We reported here two hyperacute stroke patients who emergent CAS on ICAO. Methods Electronic medical charts were reviewed, assessing intracranial hemorrhage (ICH) in two hyperacute stroke patients resulting from emergent carotid artery stenting (CAS) on the occluded internal carotid artery (ICA). Results Case description:The first patient was a 60‐year‐old male who had acute right hemiparesis, aphasia, and left gaze deviation with NIHSS12. The last known normal was five hours ago. Head and neck CT angiography (CTA) showed left anterior M2 branch artery occlusion and left ICAO. Head CT perfusion (CTP) showed a small core infarct with a large perfusion mismatch. Emergent CAS was performed without distal embolic protection (DEP) and followed by distal mechanical thrombectomy (MT). TICI 2B recanalization was achieved. After CAS, aspirin and clopidogrel were administrated. He had a large left MCA and PCA stroke from fetal PCA. A few days later, the patient developed large intraparenchymal hemorrhage (IPH) and intraventricular hemorrhage (IVH). He expired shortly. The second was a 52‐year‐old male had acute right facial droop, aphasia, dysarthria, and decreased consciousness (NIHSS 8). CTA showed left ICAO but patent intracranial arteries. CT perfusion showed a large mismatch without core infarction. He received intravenous tPA and had emergent CAS with a DEP. Aspirin 600 mg was administrated afterward. A few hours later, he had worsened weakness. Head CT showed left IPH, IVH, and subarachnoid hemorrhages with cerebral edema, and midline shift. He was medically managed for a prolonged stay and was discharged to a rehabilitation facility. Conclusions We presented two consecutive cases of emergent revascularization of ICAO in hyperacute stroke carried a high risk of ICH with poor outcome. Our online database search found that only a few case series of emergent CAS on ICAO were reported. Overall, emergent CAS carried about 20% risk of ICH and high mortality. Other series reported angioplasty on stenotic or occluded cervical ICA lesions with MT on distal occlusions had less hemorrhagic risk because there was no need fordual antiplatelet treatment. Most emergent CAS cases were performed on tandem occlusions for faster direct access and better efficacy of distal recanalization. A futurestudy comparing hemorrhagic risk betweenemergent CAS versus angioplasty of ICAO in patients with tandem occlusions can help to establish a standard MT protocol. For isolated ICAO with patent intracranial arteries from good collaterals, CTP may not be a good guidance tool for decision‐making of emergent CAS as it can falsely show mismatch from existing collaterals due to occlusion. A randomized clinical trial of comparison of medical management versus emergent CAS on those patients is warranted.

Introduction: Intraoral translocation of periodontal pathogens from untreated intraoral niches can lead to recolonisation and reinfection of recently treated periodontal pockets. Poor oral health hampers the patient’s Quality of Life (QoL) as it causes social isolation, inferiority and the development of psychiatric conditions. Full Mouth Disinfection (FMD) performed within 24 hours using chlorhexidine has reported significant improvements in clinical, microbiological and psychological parameters when compared to conventional periodontal treatment. Aim: To investigate the long-term effect of FMD on Oral Health-Related Quality of Life (OHRQoL) in patients with generalised chronic periodontitis. Materials and Methods: This study was a prospective clinical study conducted in the Department of Periodontology, Dr DY Patil Dental College and Hospital, Pune, Maharashtra, India, from October 2019 to December 2021. Study consisted of 60 participants who were allotted into two groups; 30 periodontally healthy volunteers (control group) and 30 patients (test group) diagnosed with generalised chronic periodontitis. The test group was asked to fill out the Oral Health Impact Profile-14 (OHIP-14) questionnaire to assess the OHRQoL at baseline followed by FMD protocol. All the patients were kept at periodic recall, and the OHRQoL was assessed again taken after one months three months, and six months. The control group was asked to fill out the questionnaire at the baseline. Repeated measure analysis of variance with post-hoc bonferroni test was used to compare differences in variation present from baseline to one month, three months and six months. For intergroup comparison at different time intervals, an unpaired t-test was applied. Results: The age group of participants ranged from 25-55 years with a mean age of 41.3 years. Test group had 18 males and 12 females and, 20 males and 10 females were in the control group. The mean OHIP-14 score at baseline was 3.58±3.29 in the control group and 42.35±4.32 in the test group at baseline, showing a mean difference of -38.76±0.93, which was statistically significant. After one month there was a significant improvement in the OHIP-14 scores of the test group (26.35±2.60), which was further reduced to -13.58±0.81 and -9.0±0.88 by the end of three and six months. Therefore, a notable improvement in the QoL and periodontal conditions of the patients following FMD was appreciated. Conclusion: Periodontitis negatively impacts the OHRQoL. FMD provides an improvement in the perceived QoL among periodontitis patients.