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Granular cell tumor (GCT) is a rare, benign neoplasm of Schwann cell origin. GCT is composed of cells with eosinophilic granular cytoplasm. GCT presents as a solitary painless nodule. Because of their subtle clinical presentation, GCTs are often misdiagnosed. This report of a 47-year-old woman with an auricular GCT serves to highlight that complete excision and histopathological evaluation should be attempted even in apparently benign cases, to ensure complete cure.

Papillary oncocytoma is an uncommon tumor arising within the ductular cell lining of glandular structures. These tumors contain transformed epithelial cells with eosinophilic granular cytoplasm containing densely packed abnormal mitochondrias. Ocular oncocytomas are usually benign in nature, but occasionally there can be malignant transformation, with both local and distant spread. We are reporting a case of papillary oncocytoma, who was managed with surgery without any recurrence on follow up. Keywords: Oxyphil adenomas, Papillary oncocytoma.

Patient Q.Q.S., female, melanoderm, 75 years old, attended the dentistry outpatient complaining of asymptomatic volume increase in lingual dorsum region 10 years ago. In anamnesis, the patient reported being a smoker and an alcoholic. At the oral examination, there was a nodular and fibrous lesion, similar in color to the adjacent mucosa on the back of the tongue, measuring approximately 2 cm in diameter. Preoperative examinations were requested, and incisional biopsy was performed. Microscopic examination revealed a tissue fragment coated with stratified keratinized squamous epithelium with no atypia, with intense proliferation of polygonal cells, with rounded, regular nuclei and abundant granular cytoplasm. The anatomopathologic examination was compatible with granular cell tumor. Contrast face tomography examination was requested, but the report is not yet available. The treatment planned by the team of dental surgeons was defined in the follow-up of the lesion every 6 months.

Oncocytic and oncocytoid lesions represent a distinct subset of salivary gland lesions. True oncocytic lesions of the salivary gland are entirely composed of oncocytes. These are characterized by the presence of abundant eosinophilic granules due to the presence of abundant cytoplasmic mitochondria. Oncocytic lesions of the salivary gland include oncocytosis, oncocytoma, and oncocytic carcinoma. In addition to the true oncocytic lesion, there exists another group of salivary gland lesions, which demonstrate cells with abundant and occasionally granular cytoplasm. These are often termed as "oncocytoid" lesions. The recently proposed Milan System for reporting salivary gland cytology clearly states that fine-needle aspiration specimens representing oncocytic/oncocytoid lesions of salivary gland cannot effectively distinguish between a nonneoplastic lesion, benign and malignant neoplasms. Therefore, most lesions lacking classic cytomorphologic features will be classified under the umbrella diagnostic term of "Salivary Gland Neoplasm of Uncertain Malignant Potential" (SUMP). In this review, we discuss and illustrate key clinicopathologic and radiologic features that can help the practicing cytopathologist narrow down the differential and provide the best management based diagnosis.

Hurthle cell neoplasms have been cytologically placed in a category distinct from follicular neoplasms in our past experience. Cytological features of hematoxylin-eosin-stained specimens diagnosed as “suspected Hurthle cell neoplasms” include that the aspirate is generally high cellular, is comprised exclusively of Hurthle cells, has well-defined cell borders with enlarged nuclei, has abundant granular cytoplasm, and has round, oval to polygonal nuclei sometimes with prominent macronucleoli. The background is usually clean or bloody with or without histiocytes. Hurthle cell metaplasia of follicular cells is seen in several conditions affecting the thyroid, displaying a range of cytological features that mimic those encountered in Hurthle cell neoplasms, constituting important diagnostic pitfalls. An admixture of regular follicular cells, oncocytes with highly pleomorphic in size, transition forms, degenerative changes with histiocytes, lymphocytes or colloid in the background, absent macronucleus could serve as the clues to the diagnosis of nonneoplastic Hurthle cell nodules.

Fewer than 90 cases of granular cell tumour (GCT) of the biliary tract have been reported, including only five cases of multiple GCTs. We present the unusual case of a 40-year-old woman with multifocal GCTs affecting the intrahepatic biliary tree, which were initially suspected to be hepatic multiple metastases from a malignancy of unknown origin. The surgical specimen consisted of a hepatic segment in which five whitish nodular lesions were observed. On microscopic examination, nodular lesions were found in the portal tracts; these were composed of large polygonal cells with abundant highly granular cytoplasm. The nuclei were small and centrally located. The tumour cells tested diffusely positive for CD68-PGM1, S100 protein and α-inhibin, so a diagnosis of multifocal GCT of the biliary tree was made. Three years later, the patient is still alive and a MRI has shown no changes.

Introduction: Granular Cell Tumours (GCTs) can occur in any part of the body. Many a times, they occur as a small swelling, clinically suspected to be a benign process. Histologically, they are characterised by the presence of cells with abundant granular cytoplasm. Aims: To study the clinicopathological spectrum of GCTs diagnosed. Materials and Methods: Data were collected from the archives of the Department of Pathology, on diagnosed cases of GCTs for a period of five years; from 2012 to 2017 and a total of 22 cases were included in the study. The slides, including special stains and Immunohistochemistry (IHC), whenever performed, were retrieved and studied. The results were tabulated and analysed. Results: In the present study 22 cases of GCT were described, commonly involving skin and subcutaneous tissue. Most of the lesions were less than a centimeter in size. Females were more often affected. On follow-up, no recurrence was noted. Conclusion: Granular cell tumours should be a part of the diagnostic differentials whenever lesions having cells with granular cytoplasm are encountered.

espanolResumen: En 2011 se efectuo una exploracion nematologica a la zona agricola del Valle de Tepeaca, municipio de Acatzingo en el estado de Puebla, Mexico. En una parcela cultivada con betabel ubicada en la localidad de Santa Maria Actipa, municipio de Acatzingo se encontraron, en las raices del cultivo, agallas prominentes. Se tomo una muestra (suelo + plantas) en dicha parcela, detectandose, en el laboratorio hembras de Meloidogyne en las raices, se procedio a la identificacion de la especie utilizando la prueba de fenotipos isoenzimaticos de esterasa (EST) y malato deshidrogenasa (MDH) y se estudiaron los patrones perineales de las hembras. Adicionalmente se evaluaron las alteraciones histologicas causadas por el nematodo en el interior de las raices. Los fenotipos isoenzimatico para EST y MDH fueron I1 y N2a, respectivamente, correspondientes a la especie Meloidogyne incognita y los patrones perineales de las hembras mostraron arco dorsal alto y cuadrado, sin lineas laterales claramente visibles, caracteristicos de la misma especie. Por su parte, el estudio histologico mostro hiperplasia e hipertrofia celular que provoco la formacion de cinco a siete celulas gigantes multinucleadas, con paredes celulares engrosadas y citoplasma denso y granuloso. Tambien se observaron de cuatro a seis hembras piriformes por agalla, embebidas en el tejido parenquimatoso, con disposicion cercana al cilindro vascular. La presencia de estas hembras provoco el desplazamiento de los vasos xilematicos y lignificacion de celulas aledanas. EnglishAbstract: In 2011, a nematological exploration was carried out in the agricultural area of ​​the Tepeaca Valley, Municipality of Acatzingo, in the state of Puebla, where a plot planted with beet was found in the town of Santa Maria Actipa, municipality of Acatzingo, Puebla, Mexico, where a high percentage of the roots presented prominent galls. A sample (soil + plants) was taken, in the laboratory females of Meloidogyne was detected, the identification of the nematode was carried out by the isozyme phenotype test using the enzymes esterase (EST) and malate dehydrogenase (MDH) and confirmed by morphological characters of the female perineal models. Additionally, the histological alterations were evaluated. The results showed that the isoenzymatic phenotype for EST corresponded to type I1; while for the MDH it was the N2a phenotype both associated with the specie Meloidogyne incognita. Likewise, perineal females models showed a high and square dorsal arch, with no clearly visible lateral lines those are characteristics of this species. On the other hand, the histological study showed hyperplasia and cellular hypertrophy that caused the formation of five to seven multinucleated giant cells, with thickened cell walls and dense and granular cytoplasm. Four to six piriform females per gall, embedded in the parenchymal tissue, were also observed near the vascular cylinder. The presence of these females provoked the displacement of the xylem vessels and the lignification of neighboring cells.

The effects of feeding imidacloprid were assessed in the liver of Japanese quails and studied the effect of feed mix of Butea monosperma on ameliorating effect against the imidacloprid toxicity. The serum biochemical assay revealed significant increase in AST, ALT and GGT in imidacloprid treated birds. Histologically, the liver showed sinusoidal dilatation, bile duct hyperplasia and periportal necrosis. Ultra thin sections of liver revealed numerous fat globules, condensed hepatocytes and mitochondria with granular cytoplasm. It was observed that co-treatment with Butea monosperma moderately restored the imidacloprid induced changes.

Gastrointestinal granular cell tumors (GCTs), usually benign, soft-tissue tumors, are thought to arise from Schwann cells that may occur at many sites. Only 5-7% of these lesions are detected in the gastrointestinal tract. Histologically, it is composed of sheets or nests of plump round or polygonal cells having abundant slightly amphophilic granular cytoplasm with centrally located uniform pyknotic nuclei and immunohistochemical staining for S-100 protein supports the proposed derivation from Schwann cells. In this study, we reported a case of a solitary GCT of the stomach that was completely removed after endoscopic submuscosal resection.

Background: On in vitro expansion for therapeutic purposes, the regenerative potentials of mesenchymal stem cells (MSCs) decline and rapidly enter pre-mature senescence probably involving oxidative stress. To develop strategies to prevent or slow down the decline of regenerative potentials in MSC culture, it is important to first address damages caused by oxidative stress-induced premature senescence (OSIPS). However, most existing OSIPS study models involve either long-term culture to achieve growth arrest or immediate growth arrest post oxidative agent treatment and are unsuitable for post-induction studies. Methods: In this work, we aimed to establish an OSIPS model of MSCs derived from Wharton's Jelly by hydrogen peroxide (H2O2) treatment. Results: The optimal H2O2 concentration was determined to be 200 µM to achieve OSIPS when MSC reached growth arrest in 3 to 4 passages post-H2O2 treatment. H2O2-treated cells became heterogeneous in morphology, and were irregularly enlarged and flattened with granular cytoplasm. The cells were stained positive for SA-β-galactosidase, a senescence marker, and were shown to express elevated levels of other well-characterized senescence molecular markers, including p53, p21, p16 and lysosomal β-galactosidase (GLB1) in real-time RT-PCR analysis. The OSIPS-like features were confirmed with three independent WJ-MSC lines. Conclusion: The establishment of an OSIPS model of WJ-MSC is a first step for subsequent investigation on molecular mechanisms of senescence and for screening potential anti-oxidative agents to delay or revert stressed-induced senescence.

Oncocytic tumors are characterized by an excessive eosinophilic, granular cytoplasm due to aberrant accumulation of mitochondria. Mutations in mitochondrial DNA occur in oncocytic thyroid tumors, but there is no information about their lipid composition, which might reveal candidate theranostic molecules. Here, we used desorption electrospray ionization mass spectrometry (DESI-MS) to image and chemically characterize the lipid composition of oncocytic thyroid tumors, as compared with nononcocytic thyroid tumors and normal thyroid samples. We identified a novel molecular signature of oncocytic tumors characterized by an abnormally high abundance and chemical diversity of cardiolipins (CL), including many oxidized species. DESI-MS imaging and IHC experiments confirmed that the spatial distribution of CLs overlapped with regions of accumulation of mitochondria-rich oncocytic cells. Fluorescent imaging and mitochondrial isolation showed that both mitochondrial accumulation and alteration in CL composition of mitochondria occurred in oncocytic tumors cells, thus contributing the aberrant molecular signatures detected. A total of 219 molecular ions, including CLs, other glycerophospholipids, fatty acids, and metabolites, were found at increased or decreased abundance in oncocytic, nononcocytic, or normal thyroid tissues. Our findings suggest new candidate targets for clinical and therapeutic use against oncocytic tumors. Cancer Res; 76(22); 6588–97. ©2016 AACR.

OBJECTIVE The present study consists of quality comparison among oocytes retrieved from women under 37 years old showing increased levels of FSH (prone to premature ovarian insufficiency) and women at the same age with normal hormone levels. METHODS Oocyte quality was accessed according to Lucinda L. Veeck parameters (1986) and the statistical analyses were carried out using Chisquared, SPSS for Windows 13.0 (SPSS, Inc., Chicago, IL). All pvalues were twotailed, and 95% confidence intervals (CIs) were calculated. A P value

Granular cell tumor (GCT) is an extremely uncommon entity first described by Abrikossoff in 1926 when he reported a series of five patients with such tumors found in the tongue [1], and he was also the first in reporting GCTexistence in the esophagus 5 years later. GCTs are soft-tissue neoplasms composed of Schwann cells with granular cytoplasm [2] and are mainly asymptomatic. In the gastrointestinal (GI) tract, they occur at a rate of 6 to 8% [3, 4] and especially their occurrence in esophagus is even rarer, accounting for approximately 2 % of all GCTs [4]. Herein, we present a middle-agedmale patient in whom, incidentally, a GCTwas discovered in distal part of esophagus.

Oncocytic tumors, composed of eosinophilic, mitochondria-rich cells, can occur in several locations throughout the body. These tumors can occur in the adrenal cortex and are rarely malignant. We report a case of a patient presenting with an incidental adrenal mass which was later diagnosed as a oncocytic adrenocortical neoplasm (OAN). The patient is a 53-year-old man found to have a 7.2 cm right adrenal mass, incidentally found by computed tomography (CT). After metabolic workup was negative, a right robotic adrenalectomy (RA) was performed. Pathologic analysis revealed clusters of large cells with abundant eosinophilic and granular cytoplasm, consistent with OAN. This pathology is rare, with only about 150 cases described in the literature. It occurs in females 2.5 times more frequently and more commonly on the left side. Diagnosis is usually made by imaging criteria, typically with CT or magnetic resonance imaging (MRI). Treatment is generally surgical, since OAN can be malignant in some cases. Differentiation between benign and malignant OAN is done based on the Lin-Weiss-Bisceglia criteria and can be difficult. If malignancy is diagnosed, recurrence is common and close surveillance should be performed.

Bone xanthomas are rare and are usually are associated with endocrine or metabolic diseases, mainly lipid disorders. In the absence of systemic diseases, the lesion is called a primary xanthoma. Primary mandibular xanthomas are extremely rare. The aim of this report is to describe the clinical and radiographic findings of a primary mandibular xanthoma, discussing the epidemiological features, pathogenesis and differential diagnosis. A 25-year-old man was referred for evaluation of a left mandibular lesion detected in a routine radiographic exam. Radiographically, there was a diffuse, unilocular and radiolucent lesion, with irregular margins located adjacent to the surface from the distal root of the left mandibular third molar. The lesion was excised under local anaesthesia. Microscopically, there were several cells with a foamy and granular cytoplasm and central small, round nuclei, similar to xanthomatous macrophages. No lipid disorders were diagnosed. According to these features, the diagnosis of primary mandibular xanthoma was established. In conclusion, xanthomas of the jaws are rare and all seem to be primary and occur exclusively in the mandible.

We report a rare pediatric case of granular cell tumor (GCT) arising from the subglottis. A 10-year-old boy seen for hoarseness was found in flexible fiberscopic examination to have a submucosal mass below the left vocal cord. The tumor was completely removed in direct laryngoscopy under general anesthesia. Histologically, the tumor showed the proliferation of large cells with abundant eosinophilic granular cytoplasm and oval nuclei and a surface entirely covered by stratified squamous epithelium. Immunohistochemically, S-100 protein was positive. Although a polypoid mass was seen 3 months after surgery, reexcision confirmed it to be postoperative granulation eliminating the possibility of recurrence. He remains free of recurrence 29 months after surgery. The number of pediatric laryngeal GCT reported appears to be increasing, possibly due to progress in indirect laryngoscopic devices.

Background Oncocytic carcinoid tumor of the lung is a rare variant of pulmonary carcinoid. This report describes the morphologic appearance of this rare tumor on filter membrane preparation along with potential pitfalls. Case A 49-year-old woman presented with cough and expectoration. On chest radiograph a mass lesion was seen in the upper zone of the right lung. Bronchial washings were sent for evaluation. On filter membrane (Millipore) preparation of bronchial washings the possibility of a non-small cell carcinoma, possibly squamous, was suggested. Right upper lobectomy was subsequently performed and a histologic diagnosis of oncocytic carcinoid given. The cytomorphologic features of this tumor on the Millipore preparation were reviewed. Conclusion Differential diagnosis of oncocytic carcinoid should be kept in mind while assessing cytologic material when tumor cells show abundant granular cytoplasm and prominent nucleoli. Oncocytic carcinoid also must be differentiated from oncocytoma and granular cell tumor. Immunocytochemistry and electron microscopy are useful in confirming the diagnosis.

Background: We report a case of polymorphous low-grade adenocarcinoma (PLGA) of the palate showing features similar to adenoid cystic carcinoma (ACC) in smear cytology.Case and Conclusion: Imprint specimens from the cut surface of biopsied tissue in a 78-year-old woman with a painless mass in the palate contained many neoplastic cells arranged in tight clusters, and mucous ball-like structures, pseudopapillary, tubular, and solid patterns were observed. Cells forming these clusters varied in size and had abundant eosinophilic, granular cytoplasm. Nuclei were round to oval and had a relatively smooth configuration. Although ACC was suspected cytologically, PLGA was confirmed by histopathological section. As opposed to imprint specimens obtained from resected tumors, no indian file arrays or concentric targetoids were detected in the imprint from the biopsied specimen, which thus led to diagnostic failure.

Background Breast carcinomas composed predominantly or exclusively of cells with foamy and/or granular cytoplasm have been teamed histiocytoid breast carcinoma (HBC). Cases Three cases of HBC had fine needle aspirates that were moderately cellular and composed of cells with abundant foamy and/or granular cytoplasm, arranged in loosely cohesive groups and dispersed singly. The cells shirked subtle cytologic atypia, including nuclear hyperchromasia and slightly irregular nuclear outlines. Definitive cytologic diagnosis was not possible in the ) cases, and they were reported as "suspicions for malignancy." Core biopsies of 2 cases showed a typical Indian file pattern of invasive lobular carcinoma, while the third case was composed of sheets of discohesive histiocytoid cells admixed with a prominent lymphoid infiltrate. All 3 cases were E-cadherin negative, confirming their lobular nature. Conclusion HBC represents an unusual morphologic pattern of apocrine change that may be seen in lobular and ductal breast carcinomas. Recognition of these lesions is vital in that they may he mistaken for a variety of other entities composed of foamy/granular cells; some of those entities have vastly different implications for treatment and prognosis.

Oncocytoma is an uncommon neoplasm with a characteristic histologic feature of abundant eosinophilic granular cytoplasm. It almost always occurs in kidney, thyroid, or salivary gland. Pulmonary oncocytoma is a rare pulmonary neoplasm, with fewer than 10 cases reported so far in the available English literature. We encountered one such case that was successfully managed by thoracoscopic lobectomy.

We report a case in a female newborn infant of multiple congenital epulis, i.e. granular cell tumor, that was undetected during regular pregnancy ultrasound monitoring. At birth the neoplasms appeared as two voluminous lesions protruding from the newborn's mouth. The greater of them (5.5 cm x4 cm x3 cm) was pedunculated and attached to the external superior gingiva, shifting the alae nasi and making it difficult to enter the coanae. The second mass was somewhat smaller (3 cm x4 cm x2.5 cm), pedunculated and attached to the external inferior gum. A third smaller mass was less evident, unpedunculated and attached to the rim of the lower gingiva. Histologically the lesions were characterized by large cells, which had abundant pale acidophilic granular cytoplasm. A round-oval nucleus was located centrally. The cell membranes were distinct. Neither mitosis nor necrosis was found. Staining for cytoplasmic granules was intensely periodic acid-Schiff (PAS) positive and diastase resistant. Immunohistochemical negativity for S100 protein, positivity for lysozyme and numerous phagolysosomes in the cytoplasm of neoplastic elements, observed on ultrastructural examination, supported the hypothesis that the congenital type of granular cell tumor cannot have a Schwannian origin like that of the adult type, but is probably a mesenchymal lesion which, for unknown cause, regresses by a degenerative process.

We encountered a rare case of oncocytoma of the submandibular gland.A 73-year-old man complained of a gradually growing mass in the right submandibular region. The tumor was elastic soft and mobile, with a clear margin. Echography revealed a hypoechoic lesion, CT scans showed a welldefined homogenous submandibular mass, and MRI scans demonstrated a high-intensity mass on Ti and T2-weighted images in the right submandibular region. The tumor exhibited an intense uptake of 99mTc pertechnatate. Histopathological examination showed that the tumor cells consisted of large cells with eosinophilic granular cytoplasm. On the basis of these findings, we diagnosed the tumor as an oncocytoma.The postoperative course has been uneventful for 9 years.

Synovial sarcoma (SS) is a high-grade sarcoma that can be diagnosed in cytology with certainty only when it presents with a biphasic pattern. Monophasic SS (MSS), however, is a diagnostic consideration when a uniform spindle cell population is present. The purpose of this study was to evaluate a series of cytologic cases of MSS and its cytologic presentation. Twenty-one FNAs of histologically confirmed MSS were reviewed. Specimens consisted of tissue fragments and single cells containing scant granular cytoplasm, medium-sized nuclei, and coarse chromatin. A monotonous spindle pattern with comma-shaped nuclei was present in 5 cases. Sixteen cases contained oval and spindled nuclei. Eight of these specimens contained round nuclei, and 5 of these cases showed prominent nucleoli and cohesive clusters, reminiscent of biphasic SS. We conclude that a spectrum of cytologic findings can be seen in MSS, including a secondary population of cells with morphology usually typical of biphasic SS. Diagn. Cytopathol. 2004;30:19–23. © 2004 Wiley-Liss, Inc.

A 53 year-old woman presented with a slowly progressive, painless proptosis OS. Computed tomography disclosed a round, homogeneous, well-delimited lesion in the inferior-temporal orbit. The tumor was composed of round cells with eosinophilic granular cytoplasm. Some of the cells had larger eosinophilic granules surrounded by a clear halo; known as pustulo-ovoid bodies of Milian or Bangle bodies. The diagnosis of a granular cell tumor was then established and confirmed by immunohistochemistry. Granular cell tumors are uncommon benign soft tissue neoplasms that have a predilection for the head and neck region. Awareness of the typical histopathological features is crucial for the correct diagnosis.

Oncocytic carcinoma is an extremely rare neoplasm of the salivary gland, with only a few cases reported in literature till date. We report the occurrence of this rare lesion in lip in a 43-year-old female presenting with a progressively increasing swelling for which excision was done. Fine needle aspiration was done and the smears revealed tumor cells with well-defined cell borders, round to oval, central to eccentrically located moderately pleomorphic nuclei with fine chromatin, prominent nucleoli and abundant eosinophilic granular cytoplasm. Microscopic examination of the resected tumor showed solid sheets, nests, islands and cords of oncocytic cells diffusely infiltrating the surrounding tissues. After 5 months, the patient again presented with bilateral submandibular and right axillary lymphadenopathy revealing metastatic deposits from oncocytic carcinoma. We report this case of oncocytic carcinoma because of its unusual location, the minor salivary gland of lip being a rare site for the tumor.

Enhanced surface photooxidation property associated with the ZnS nanoparticles caused the reduction of dissolved oxygen content in water in a dose dependent manner, when ZnS nanoparticles of different sizes are exposed to the water in various concentrations. This property was more prominent for ZnS nanoparticles with smaller sizes.Mystus tengara, exposed to ZnS nanoparticles, responded to hypoxia with varied behavioural, physiological, and cellular responses in order to maintain homeostasis and organ function in an oxygen-depleted environment. The histomorphology of corpuscles of Stannius of the fish showed conspicuous vicissitudes under exposure of ZnS nanoparticles. The population of the cell type with granular cytoplasm showed significant increase at the expense of the other that consisted of agranular cytoplasm with increasing nanoparticle concentration. This can be explained as the defence mechanism of the fish against ZnS nanoparticle induced hypoxia and environmental acidification. The altering histomorphology has been studied employing an analytical approach.

Mammary analogue secretory carcinoma (MASC) is a recently recognized tumor of salivary glands characterized by the ETV6-NTRK3 fusion gene. This tumor is very rare in children and adolescents. We report a case of MASC in a 15-year-old girl, the fifth youngest case so far reported. The patient complained of a left infra-auricular mass that gradually enlarged for a year. Fine-needle aspiration cytology/imprint cytology showed individual tumor cells that had faintly eosinophilic granular cytoplasm with secretion granules sometimes seen adjacent to the tumor cells. These cytological features overlapped between those of zymogen granule-poor acinic cell carcinoma (AciCC) and MASC. In addition to the case report, we present a review of the related literature with a focus on the cytological features of MASC. The differential diagnostic clues are also discussed.

Summary Oncocytic meningioma is a recently described rare variant of meningothelial neoplasms that typically occurs as a large cell tumor with granular cytoplasm. The distinct histological features of numerous cells with granular cytoplasm and the ultrastructural evidence of numerous mitochondria in the cytoplasm differentiate this tumor from other neoplasms with granular appearance. We report an additional case of oncocytic meningioma investigated by ultrastructural and immunohistochemical methods.

目的:唾液腺穿刺吸引細胞診の有用性について検討し, さらに穎粒状細胞質を示す病変について, 細胞学的鑑別点を明らかにする.対象および方法:1994年から1999年に山口県立中央病院で唾液腺穿刺吸引細胞診を行い, 組織像との比較が可能であった73例を対象にした.(1) 細胞診での推定病変と組織診断の不一致例の細胞像を再検討し, 不一致の原因を検討した.(2) 顆粒状細胞質を示す病変については, 出現細胞を好酸性細胞と腺房細胞に大別し, その鑑別点について検討した.結果:(1) 推定病変と組織診断の一致率は84.9%, 良悪性の一致率は97.3%であった. 不一致の原因は細胞像把握ミスが6例, 病変推定困難が5例であった.(2) 好酸性細胞と腺房細胞の鑑別において, 前者で細胞質内顆粒が大きく, 分布が均等で密であった. 後者では細胞境界が明瞭で, 出現細胞間で細胞質所見に差がみられた.結論:唾液腺穿刺吸引細胞診は, 高い精度をもって, 病変推定が可能である. 顆粒状細胞質を示す病変の推定に関しては, 好酸性細胞と腺房細胞を, 細胞質の詳細な観察により鑑別することで, より正確な病変推定が可能である.

BACKGROUND: The plasmacytoid variant is a rare and controversial subtype of myoepithelioma that lacks myogenic differentation and the cytologic findings of which have not been reported previously. CASE: A 46-year-old man presented with a painless tumor located in the soft palate. Fine needle aspiration cytology (FNAC) showed odd-shaped cellular aggregates and single cells with round nuclei and finely granular cytoplasm resembling plasma cells together with strands of metachromatic stroma. The light, immunohistochemical and ultrastructural studies performed on the surgical specimen confirmed the initial cytologic diagnosis. CONCLUSION: Recognition of the cytologic findings of plasmacytoid myoepithelioma on needle aspirates allows a reliable and quick diagnosis that prompts correct management.

Oncocytic carcinoma (OC) arising in the salivary gland is a very rare tumor with only 32 previously reported cases. In this report, we describe a novel case of oncocytic carcinoma with associated thymoma, which arose in the left parotid gland of a 66-year-old male with a history of a painless left parotid mass for 1 year. Oncocytes are large, polygonal cells that are characterized by marked cellular atypia, frequent mitoses, wide eosinophilic granular cytoplasm, a central nucleus and a prominent nucleolus. The follow-up data showed no evidence of recurrence and the patient is in a good health 20 months after the surgery. In the current case, the patient had not only OC but also thymoma, which is exceedingly rare and may represent the first documented case in the literature.

Objectives: The tall cell variant of papillary thyroid carcinoma (TCV-PTC) is an aggressive variant of PTC requiring accurate cytopathologic diagnosis for early aggressive management. Study Design: Twenty-five cases of TCV-PTC in which the tall cells comprised at least 30% of surgically resected tumor were included in the study. The direct smears from a preoperative fine needle aspiration (FNA) and available hematoxylin and eosin cell block sections were reviewed. Ten cases of TCV-PTC were randomly selected and blinded with an equal number of conventional PTC cases. Representative slides were independently reviewed by 7 cytologists. Results: In a majority of the cases, the FNA direct smears were hypercellular and displayed flat monolayer sheets of cells. Tall columnar cells with cytoplasmic tails were seen in 56% of cases. The presence of large polygonal follicular cells with abundant granular oncocytic cytoplasm and distinct cell borders was the most common feature seen in all cases. Seventeen (68%) cases displayed intranuclear pseudoinclusions in cells with abundant granular cytoplasm. A correct diagnosis of TCV-PTC was made in 30-40% of cases by 7 study participants. Conclusions: The correct recognition of TCV-PTC features in preoperative FNA is important for clinical management, and reporting these features in a cytopathology report is suggested.

Degranulation of intestinal mast cells in rainbow trout (Oncorhynchus mykiss Walbaum) was studied by means of image analysis technique. Two strips from the same intestinal segment from ten clinically healthy trout were sampled. One was immediately mounted in an organ bath, treated with compound 48/80 and then processed, as the remnant untreated strip, for light microscopy. Colour pictures taken from each section were converted in their gray levels RGB stacks equivalent and in 8 bits gray levels. Five granular cytoplasm areas of mast cells (MCs) for each section were analysed for the following parameters: mean gray values and Gray Level Correlation Matrices parameters. All RGB channels and the 8 bit gray levels transformed images showed higher mean gray level values after compound 48/80 exposure (Anova, p0.01). Only two channels (Red and Green) and the 8 bit gray levels transformed images of a unique texture parameter (contrast) appeared to be significantly higher after compound 48/80 exposure (Anova, p0.05). Red channel and Blue channel means gray level values were able to discriminate between not degranulated and degranulated MCs. The described model represents a promising alternative in mast cell research.

Note Oncocytes (from the greek word "swell"), also known as Hurthle cells, oxyphilic cells or Askanazy cells, are characterized by abundant granular cytoplasm due to aberrant accumulation of mitochondria. The cause for the mitochondria accumulation is unknown. The increased number of mithochondria may be a compensatory to intrinsic defects in the energy production machinery of the cell. Cells that fit this basic description may be identified in a variety of nonneoplastic lesions as well as in the affected tissues of patients with mitochondrial myopaties. Tumors composed of oncocytes are not restricted to the thyroid gland and may arise in a variety of locations, most commonly in the kidney and salivary gland and may be benign or malignant.

Fine-needle aspiration (FNA) diagnosis of oncocytic lesions is challenging. In fact, oncocytic changes occur in inflammatory, hyperplastic, and neoplastic settings, including both benign and malignant tumors. The rare oncocytic variant of papillary thyroid carcinoma (PTC), shows papillae composed by cells with large oncocytic granular cytoplasm featuring clear PTC nuclear features. A morphological similar, but biologically distinct lesion, is the encapsulated papillary oncocytic neoplasia. Here, we first report on FNA, its cytological features together with histological, immunohistochemical, and molecular correlates. Diagn. Cytopathol. 2011;. © 2011 Wiley-Liss, Inc.

A case of alveolar soft-part sarcoma diagnosed by fine needle aspiration cytology is reported. The cytologic findings from both Diff-Quick and Pananicolaou stained slides showed cells arranged in both clusters and individually, with rare groups of cells mimicking an alveolar pattern. The individual cells showed minimal nuclear pleomorphism and eccentrically placed vesicular nuclei; some had 1 to 2 small prominent nucleoli and moderate amounts of granular cytoplasm. These features are suggestive of alveolar softpart sarcoma in the appropriate clinical setting. The cytopathologic diagnosis was subsequently confirmed by the histopathologic study of the cell block preparation and by the electron microscopy submitted from the aspirated material. (The J Histotechnol 16:375, 1993)

Tumors with oncocytic features are characterized histologically by cells with eosinophilic granular cytoplasm and by the ultrastructural presence of numerous closely packed mitochondria. Adrenal oncocytic tumors are very rare. We describe the case of a 36-year old white male who was newly diagnosed with hypertension and referred for evaluation. An abdominal computer tomography scan revealed a right adrenal tumor, which was removed surgically. The final diagnosis was made histologically on the excised tumor specimen. Hypertension in this patient was cured by the removal of the tumor. Adrenal oncocytic tumors are often benign, non-functional tumors that rarely cause hypertension. The diagnosis and assessment of metastatic potential can be made with histology and appropriate immunohistochemical stains. Removal of the tumor is necessary as some may have a malignant potential. Long-term follow-up is important in these patients in view of the difficulty in accurately predicting biological behavior.