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Members of the bacterial candidate phylum WPS-2 (or Eremiobacterota) are abundant in several dry, bare soil environments. In a bare soil deposited by an extinct iron-sulfur spring, we found that WPS-2 comprised up to 24% of the bacterial community and up to 108 cells per g of soil based on 16S rRNA gene sequencing and quantification. A single genus-level cluster (Ca. Rubrimentiphilum) predominated in bare soils but was less abundant in adjacent forest. Nearly complete genomes of Ca. Rubrimentiphilum were recovered as single amplified genomes (SAGs) and metagenome-assembled genomes (MAGs). Surprisingly, given the abundance of WPS-2 in bare soils, the genomes did not indicate any capacity for autotrophy, phototrophy, or trace gas metabolism. Instead, they suggest a predominantly aerobic organoheterotrophic lifestyle, perhaps based on scavenging amino acids, nucleotides, and complex oligopeptides, along with lithotrophic capacity on thiosulfate. Network analyses of the entire community showed that some species of Chloroflexi, Actinobacteria, and candidate phylum AD3 (or Dormibacterota) co-occurred with Ca. Rubrimentiphilum and may represent ecological or metabolic partners. We propose that Ca. Rubrimentiphilum act as efficient heterotrophic scavengers. Combined with previous studies, these data suggest that the phylum WPS-2 includes bacteria with diverse metabolic capabilities.

A causal mechanism for the Volgian Isotopic Carbon Excursion (VOICE) remains enigmatic. Elemental geochemical profiles of the Deer Bay Formation, Sverdrup Basin, Arctic Canada that record the VOICE and contemporaneous strata are herein examined to provide insight into depositional environments during Late Jurassic‐Early Cretaceous time. Silver (Ag) and Cadmium (Cd) are enriched across the VOICE at localities on Axel Heiberg Island, and in Tithonian (∼Volgian) strata of Ellef Ringnes Island. Other redox‐sensitive trace elements do not exhibit spatially or temporally consistent patterns and indicate oxic conditions. A lack of relationship across the VOICE between Ag and the quality, quantity, and isotopic composition of organic matter suggests that the negative isotope excursion and interval of Ag enrichment are not merely functions of changes in organic matter source or amount, while a lack of spatially consistent change in geochemical indices of weathering similarly excludes climate change and/or sediment provenance as a driver. Therefore, in a ventilated setting and without marked changes in organic matter content, Ag enrichment may be due to hydrothermal activity. Contemporaneous Ag enrichment in strata from Svalbard suggests that a source of hot fluid sufficient to produce Ag‐rich seawater may have been related to rifting in the adjacent proto‐Amerasia Basin. Hydrothermal activity may also have been a widespread source of isotopically depleted carbon. This work develops new geochemical fingerprints that may be used to trace the spatial extent of hydrothermal events that do not leave an extinction pattern but may nonetheless have a far‐reaching influence on biogeochemical systems. Plain Language Summary: Silver (Ag) is enriched in marine sedimentary strata of Tithonian age, and in particular, across the Volgian Isotopic Carbon Excursion in Arctic Canada. However, other redox‐sensitive trace elements do not exhibit spatially or temporally consistent patterns, and collectively suggest oxygenated conditions near the seafloor during this time. Contemporaneous Ag enrichment in strata from Svalbard suggests that a source of hot fluid sufficient to produce Ag‐rich seawater may have been related to rifting in the adjacent proto‐Amerasia Basin. Hydrothermal activity may also have been a widespread source of isotopically depleted carbon. This work develops new geochemical fingerprints that may be used to trace the spatial extent of hydrothermal events that do not leave an extinction pattern but may nonetheless have a far‐reaching influence on biogeochemical systems. Key Points: Silver is enriched across the Volgian Isotopic Carbon Excursion (VOICE) in the Canadian ArcticOther redox‐sensitive trace elements do not exhibit spatially or temporally consistent patterns and indicate oxic conditionsRifting in the proto‐Amerasia Basin may have resulted in hydrothermal activity that caused the silver enrichment [ABSTRACT FROM AUTHOR]

In this study, we describe a deep learning (DL)-based workflow for the three-dimensional (3D) geophysical inversion of magnetotelluric (MT) data. We derived a mathematical connection between a 3D resistivity model and the surface-observed electric/magnetic field response by using a fully connected neural network framework (U-Net). Limited by computer hardware functionality, the resistivity models were generated by using a random walk technique to enlarge the generalization coverage of the neural network model, and 15,000 paired datasets were utilized to train and validate it. Grid search was used to select the optimal configuration parameters. With the optimal model framework from the parameter tuning phase, the metrics showed stable convergence during model training/validation. In the test period, the trained model was applied to predict the resistivity distribution by using both the simulated synthetic and the real MT data from the Mount Meager area, British Columbia. The reliability of the model prediction was verified with noised input data from the synthetic model. The calculated results can be used to reconstruct the position and shape trends of bodies with anomalous resistivity, which verifies the stability and performance of the DL-based 3D inversion algorithm and showcases its potential practical applications. [ABSTRACT FROM AUTHOR]

Analysis of the increasing wealth of metagenomic data collected from diverse environments can lead to the discovery of novel branches on the tree of life. Here we analyse 5.2 Tb of metagenomic data collected globally to discover a novel bacterial phylum ('Candidatus Kryptonia') found exclusively in high-temperature pH-neutral geothermal springs. This lineage had remained hidden as a taxonomic 'blind spot' because of mismatches in the primers commonly used for ribosomal gene surveys. Genome reconstruction from metagenomic data combined with single-cell genomics results in several high-quality genomes representing four genera from the new phylum. Metabolic reconstruction indicates a heterotrophic lifestyle with conspicuous nutritional deficiencies, suggesting the need for metabolic complementarity with other microbes. Co-occurrence patterns identifies a number of putative partners, including an uncultured Armatimonadetes lineage. The discovery of Kryptonia within previously studied geothermal springs underscores the importance of globally sampled metagenomic data in detection of microbial novelty, and highlights the extraordinary diversity of microbial life still awaiting discovery.