4 results on '"Gregor Bötticher"'
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2. Aneurysmen der Nieren- und Viszeralarterien
- Author
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Gregor Bötticher
- Published
- 2022
- Full Text
- View/download PDF
3. Long-Term Quality of Life of Abdominal Aortic Aneurysm Patients Under Surveillance or After Operative Treatment
- Author
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Herold Kuffner, Irene Hinterseher, Diane T. Smelser, Hendrik Berth, Gregor Bötticher, Gabor Gäbel, and Hans Detlev Saeger
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,Population ,Endovascular aneurysm repair ,Blood Vessel Prosthesis Implantation ,Aortic aneurysm ,Quality of life ,Surveys and Questionnaires ,medicine ,Humans ,cardiovascular diseases ,education ,Aged ,Aged, 80 and over ,education.field_of_study ,business.industry ,Endovascular Procedures ,General Medicine ,Middle Aged ,medicine.disease ,humanities ,Abdominal aortic aneurysm ,Surgery ,Elective Surgical Procedures ,Quality of Life ,cardiovascular system ,Female ,Cardiology and Cardiovascular Medicine ,Elective Surgical Procedure ,business ,Aortic Aneurysm, Abdominal ,Abdominal surgery ,Patient education - Abstract
The objective of this study was to determine the long-term quality of life (QOL) in patients with an abdominal aortic aneurysm (AAA) undergoing surveillance or after operative treatment.249 patients with AAAs completed the WHO Quality of Life-BREF (WHOQOL-BREF) test and Short Form (36) Health Survey (SF-36) survey: 78 patients with small AAAs under surveillance, 26 after ruptured AAAs (rAAAs), 47 after endovascular aneurysm repair (EVAR), and 98 after elective open repair. The results were compared with WHOQOL-BREF and SF-36 standard values from a matched German population using the Student's 2-tailed t-test.Long-term results of the WHOQOL-BREF test showed that patients undergoing AAA surveillance had a significantly lower physical QOL (P = 0.04). Patients after EVAR or open repair rated their environmental QOL significantly higher than the age- and sex-matched general population (open repair: P = 0.006; EVAR: P0.001). Patients with rAAAs had the same QOL as the matched German population. Long-term results of the QOL SF-36 showed that patients undergoing AAA surveillance rated their QOL significantly lower in the subgroup of role-physical (P = 0.02) and role-emotional (P = 0.003). Patients with rAAAs rated lower scores for role-physical (P = 0.02) and had more bodily pain (P = 0.02). Patients who underwent elective open repair had the same high QOL as the matched German population, whereas patients who underwent EVAR reported significant improvement in vitality (P = 0.002) and mental health (P = 0.03) compared with the matched German population.Based on measurements from 2 independent QOL tests, the well-established operative treatment of AAAs provided patients with a QOL comparable to that of a matched German population. The electively treated AAA groups rated environmental QOL factors significantly higher than the control group. The impaired physical and emotional QOL of the AAA group under surveillance suggests that more intense patient education could be beneficial.
- Published
- 2013
- Full Text
- View/download PDF
4. Isolation of Human Islets from Partially Pancreatectomized Patients
- Author
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Michele Solimena, Gregor Bötticher, Florian Ehehalt, Klaus Peter Knoch, Gustavo B. Baretton, Stephan Kersting, Dorothée Sturm, Robert Grützmann, and Hans Detlev Saeger
- Subjects
medicine.medical_specialty ,Pathology ,medicine.medical_treatment ,General Chemical Engineering ,Cytological Techniques ,Ficoll ,030209 endocrinology & metabolism ,human islets ,General Biochemistry, Genetics and Molecular Biology ,Pathogenesis ,03 medical and health sciences ,Islets of Langerhans ,0302 clinical medicine ,Pancreatectomy ,Diabetes mellitus ,partial pancreatectomy ,Internal medicine ,medicine ,Humans ,Pancreas ,Issue 53 ,030304 developmental biology ,0303 health sciences ,geography ,geography.geographical_feature_category ,Common bile duct ,General Immunology and Microbiology ,business.industry ,General Neuroscience ,medicine.disease ,Islet ,3. Good health ,medicine.anatomical_structure ,Endocrinology ,Diabetes Mellitus, Type 2 ,Pancreatitis ,Medicine ,Digestion ,business ,human islet isolation - Abstract
Investigations into the pathogenesis of type 2 diabetes and islets of Langerhans malfunction (1) have been hampered by the limited availability of type 2 diabetic islets from organ donors(2). Here we share our protocol for isolating islets from human pancreatic tissue obtained from type 2 diabetic and non-diabetic patients who have undergone partial pancreatectomy due to different pancreatic diseases (benign or malignant pancreatic tumors, chronic pancreatitis, and common bile duct or duodenal tumors). All patients involved gave their consent to this study, which had also been approved by the local ethics committee. The surgical specimens were immediately delivered to the pathologist who selected soft and healthy appearing pancreatic tissue for islet isolation, retaining the damaged tissue for diagnostic purposes. We found that to isolate more than 1,000 islets, we had to begin with at least 2 g of pancreatic tissue. Also essential to our protocol was to visibly distend the tissue when injecting the enzyme-containing media and subsequently mince it to aid digestion by increasing the surface area. To extend the applicability of our protocol to include the occasional case in which a large amount (>15g) of human pancreatic tissue is available , we used a Ricordi chamber (50 ml) to digest the tissue. During digestion, we manually shook the Ricordi chamber(3) at an intensity that varied by specimen according to its level of tissue fibrosis. A discontinous Ficoll gradient was then used to separate the islets from acinar tissue. We noted that the tissue pellet should be small enough to be homogenously resuspended in Ficoll medium with a density of 1.125 g/ml. After isolation, we cultured the islets under stress free conditions (no shaking or rotation) with 5% CO(2;) at 37 °C for at least 48 h in order to facilitate their functional recovery. Widespread application of our protocol and its future improvement could enable the timely harvesting of large quantities of human islets from diabetic and clinically matched non-diabetic subjects, greatly advancing type 2 diabetes research.
- Published
- 2011
- Full Text
- View/download PDF
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