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227 results on '"Gress, R E"'

2. Peripheral blood marker of residual acute leukemia after hematopoietic cell transplantation using multi-plex digital droplet PCR

Author

Stanojevic, M, Grant, M, Vesely, S K, Knoblach, S, Kanakry, C G, Nazarian, J, Panditharatna, E, Panchapakesan, K, Gress, R E, Holter-Chakrabarty, J, Williams, Kirsten M, Stanojevic, M, Grant, M, Vesely, S K, Knoblach, S, Kanakry, C G, Nazarian, J, Panditharatna, E, Panchapakesan, K, Gress, R E, Holter-Chakrabarty, J, and Williams, Kirsten M

Abstract

BACKGROUND Relapse remains the primary cause of death after hematopoietic cell transplantation (HCT) for acute leukemia. The ability to identify minimal/measurable residual disease (MRD) via the blood could identify patients earlier when immunologic interventions may be more successful. We evaluated a new test that could quantify blood tumor mRNA as leukemia MRD surveillance using droplet digital PCR (ddPCR). METHODS The multiplex ddPCR assay was developed using tumor cell lines positive for the tumor associated antigens (TAA: WT1, PRAME, BIRC5), with homeostatic ABL1. On IRB-approved protocols, RNA was isolated from mononuclear cells from acute leukemia patients after HCT (n = 31 subjects; n = 91 specimens) and healthy donors (n = 20). ddPCR simultaneously quantitated mRNA expression of WT1, PRAME, BIRC5, and ABL1 and the TAA/ABL1 blood ratio was measured in patients with and without active leukemia after HCT. RESULTS Tumor cell lines confirmed quantitation of TAAs. In patients with active acute leukemia after HCT (MRD+ or relapse; n=19), the blood levels of WT1/ABL1, PRAME/ABL1, and BIRC5/ABL1 exceeded healthy donors (p<0.0001, p=0.0286, and p=0.0064 respectively). Active disease status was associated with TAA positivity (1+ TAA vs 0 TAA) with an odds ratio=10.67, (p=0.0070, 95% confidence interval 1.91 - 59.62). The area under the curve is 0.7544. Changes in ddPCR correlated with disease response captured on standard of care tests, accurately denoting positive or negative disease burden in 15/16 (95%). Of patients with MRD+ or relapsed leukemia after HCT, 84% were positive for at least one TAA/ABL1 in the peripheral blood. In summary, we have developed a new method for blood MRD monitoring of leukemia after HCT and present preliminary data that the TAA/ABL1 ratio may may serve as a novel surrogate biomarker for relapse of acute leukemia after HCT.

Published
2022

5. Malnutrition in patients with chronic GVHD

Author

Bassim, C W, Fassil, H, Dobbin, M, Steinberg, S M, Baird, K, Cole, K, Joe, G, Comis, L E, Mitchell, S A, Grkovic, L, Edwards, D, Mays, J W, Cowen, E W, Pulanic, D, Williams, K M, Gress, R E, and Pavletic, S Z

Published
2014
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11. NOVEL IMAGING REVEALS EARLY ENGRAFTMENT AND STEM CELL HOMING AFTER HSCT: PH-P299

Author

Williams, K. M, Holter Chakrabarty, J. L, Lindenberg, L., Gea-Banacloche, J., Schuver, Blacklock B., Hickstein, D., Kochenderfer, J., Wilder, J., Kurdziel, K., Steinberg, S., Khuu, H., Fowler, D. H., Halverson, D., Avila, D. N., Selby, G., Taylor, T. N., Mann, J., Hsu, J., Pavletic, S. Z., Bollard, C. M., Choyke, P., and Gress, R. E.

Published
2014

18. A Large Cohort Comparison of 2005 National Institutes of Health Chronic Graft-Versus-Host Disease Staging Criteria to 2014 National Institutes of Health Chronic Graft-Versus Host Disease Staging Criteria

Author

Kerep, A. Z., Broome, J., Pirsl, F., Steinberg, S. M., Mitchell, S. A., Cowen, E. W., Pichard, D. C., Joe, G. O., Comis, L. E., Mays, J. W., Datiles, M. B., Stratton, P., Zolton, J., Berger, A., Hendricks, J., Kenyon, M., Baruffaldi, J., Denisova, M., Curtis, L. M., Pulanić, Dražen, Baird, K., Fowler, D. H., Gress, R. E., Pavletic, S. Z., and Bob Löwenberg

Subjects
chronic graft versus host disease, criteria comparison
Abstract

Usporedba NIH kriterija za kroničnu bolest davatelja protiv primatelja 2005 i 2014 godina.

Published
2017

19. Predictive models for ocular chronic graft-versus-host disease diagnosis and disease activity in transplant clinical practice

Author

Curtis, L. M., primary, Datiles, M. B., additional, Steinberg, S. M., additional, Mitchell, S. A., additional, Bishop, R. J., additional, Cowen, E. W., additional, Mays, J., additional, McCarty, J. M., additional, Kuzmina, Z., additional, Pirsl, F., additional, Fowler, D. H., additional, Gress, R. E., additional, and Pavletic, S. Z., additional

Published
2015
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20. Differential T cell hyporesponsiveness induced by in vivo administration of intact or F(ab')2 fragments of anti-CD3 monoclonal antibody. F(ab')2 fragments induce a selective T helper dysfunction

Author

Raphael Hirsch, Archibald, J., and Gress, R. E.

Subjects
Immunology, Immunology and Allergy
Abstract

Induction of peripheral T cell anergy associated with stimulation through the TCR complex in vivo has been described in mice using chemically modified APC, staphylococcal enterotoxin B, and intact anti-CD3 mAb. In the latter two models, T cell proliferation, IL-2R expression, and lymphokine production have been demonstrated before subsequent induction of hyporesponsiveness, whereas in the former model, these events have not been observed. To further investigate the relationship between mitogenicity and induction of peripheral hyporesponsiveness, mice were treated with either mitogenic intact anti-CD3 mAb or nonmitogenic F(ab')2 fragments of anti-CD3 mAb. T cells from F(ab')2-treated mice demonstrated a selective decrease in helper functions, with minimal effect on CTL function. Specifically, a marked reduction in ability of Th cells to secrete IL-2 when challenged in vitro with mitogen or alloantigen was observed, which persisted for at least 2 mo after mAb administration and which was independent of T cell depletion. Proliferative function was decreased in CD4+ T cells and could not be fully restored with addition of exogenous IL-2. A helper defect was also evident in vivo, in that F(ab')2-treated mice were deficient in their ability to reject MHC-disparate skin grafts, and in vivo administration of IL-2 reconstituted their ability to reject skin grafts normally. In contrast, T cells from mice treated with intact mAb demonstrated a significant decrease in both CTL and helper functions. A long term reduction in TCR expression on CD4+ cells from F(ab')2-treated mice, and on both CD4+ and CD8+ cells from intact mAb-treated mice was observed. These findings demonstrate that peripheral T cell hyporesponsiveness can be induced in vivo by binding an identical epitope on the TCR complex in the presence or absence of initial proliferation, lymphokine secretion, or IL-2R expression, and that binding to the same epitope can result in varying long term effects on T cell function.

Published
1991
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21. In vivo administration of anti-CD3 monoclonal antibodies or immunotoxins in murine recipients of allogeneic T cell-depleted marrow for the promotion of engraftment

Author

Blazar, B. R., Raphael Hirsch, Gress, R. E., Carroll, S. F., and Vallera, D. A.

Subjects
Immunology, Immunology and Allergy
Abstract

The role of host anti-donor cells in rejection of fully allogeneic donor T cell-depleted marrow was investigated by using mAb or immunotoxins directed against T cell or NK cell determinants. Immunotoxins consisting of mAb conjugated to a low oligosaccharide-containing fraction of purified ricin toxin A chain (RTA) facilitated in vivo-depletion of target cell populations. BALB/c and DBA/1 donors were selected based upon their expression (BALB/c) or lack of (DBA/1) hemopoietic histocompatibility (Hh1) Ag, which may serve as targets for donor rejection in C57BL/6 hosts. When studies directed toward eliminating CD3+ cells were performed in both systems, injections of intact anti-CD3 mAb or anti-CD3-RTA reproducibly produced the highest engraftment values. The fact that engraftment values obtained with anti-CD3 or anti-CD3-RTA therapy in allogeneic systems were substantially higher than in syngeneic controls suggested that engraftment stimulatory proteins were released upon TCR engagement. Elevated levels of cytokines and a high mortality rate in allogeneic recipients confirmed that this was the case. Nonstimulatory preparations of anti-CD3F(ab')2 fragments and anti-CD3F(ab')2-RTA promoted engraftment of both types of allogeneic marrow, as measured by short term 125I-IUdR assays, suggesting that stimulation was not a prerequisite for engraftment. Recipients of anti-CD3F(ab')2 or anti-CD3F(ab')2-RTA showed a marked reduction of host CD3+ cells as measured by immunofluorescence and flow cytometry. In long term chimerism studies, recipients of Hh1-disparate marrow and anti-CD3F(ab')2 had a dramatic increase in donor cell engraftment as compared to controls, indicating that positive effects on engraftment were long lived. Studies further showed that BALB/c donor cells exhibiting an Hh1 disparity were rejected by host cells expressing NK1.1 or Ly-1 (NK cells and T cells). In contrast, DBA/1 donor cells that were not Hh1-disparate were rejected by cells expressing Ly-1, but not NK1.1 (T cells only). These studies provide definitive data that CD3+ cells participate in the rejection of either Hh1+ or Hh1null T cell-depleted allografts and offer new strategies for alloengraftment using regimens containing nonmitogenic anti-CD3.

Published
1991
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22. Validation of the National Institutes of Health chronic GVHD Oral Mucosal Score using component-specific measures

Author

Bassim, C W, primary, Fassil, H, additional, Mays, J W, additional, Edwards, D, additional, Baird, K, additional, Steinberg, S M, additional, Williams, K M, additional, Cowen, E W, additional, Mitchell, S A, additional, Cole, K, additional, Taylor, T, additional, Avila, D, additional, Zhang, D, additional, Pulanic, D, additional, Grkovic, L, additional, Fowler, D, additional, Gress, R E, additional, and Pavletic, S Z, additional

Published
2013
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24. Neoantigen and tumor antigen-specific immunity transferred from immunized donors is detectable early after allogeneic transplantation in myeloma patients

Author

Foglietta, M, primary, Neelapu, S S, additional, Kwak, L W, additional, Jiang, Y, additional, Nattamai, D, additional, Lee, S-T, additional, Fowler, D H, additional, Sportes, C, additional, Gress, R E, additional, Steinberg, S M, additional, Vence, L M, additional, Radvanyi, L, additional, Dwyer, K C, additional, Qazilbash, M H, additional, Bryant, R N K, additional, and Bishop, M R, additional

Published
2012
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25. Clinical laboratory markers of inflammation as determinants of chronic graft-versus-host disease activity and NIH global severity

Author

Grkovic, L, primary, Baird, K, additional, Steinberg, S M, additional, Williams, K M, additional, Pulanic, D, additional, Cowen, E W, additional, Mitchell, S A, additional, Hakim, F T, additional, Martires, K J, additional, Avila, D N, additional, Taylor, T N, additional, Salit, R B, additional, Rowley, S D, additional, Zhang, D, additional, Fowler, D H, additional, Bishop, M R, additional, Gress, R E, additional, and Pavletic, S Z, additional

Published
2011
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28. Association of serum interleukin-7 and interleukin-15 levels with immune reconstitution (IR), graft-versus-host disease (GVHD), and survival after reduced-intensity allogeneic stem cell transplantation (RIST)

Author

Dean, R. M., primary, Hakim, F. T., additional, Fry, T., additional, Steinberg, S. M., additional, Boyiadzis, M., additional, Fowler, D. H., additional, Odom, J., additional, Mackall, C., additional, Gress, R. E., additional, and Bishop, M. R., additional

Published
2007
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33. Comparative analysis of FoxP3+ regulatory T cells in the target tissues and blood in chronic graft versus host disease.

Author

Imanguli, M M, Cowen, E W, Rose, J, Dhamala, S, Swaim, W, Lafond, S, Yagi, B, Gress, R E, Pavletic, S Z, and Hakim, F T

Subjects
T cells, INFLAMMATION, GRAFT versus host disease, CD3 antigen, CHEMOKINE receptors
Abstract

Activation and migration of regulatory T cells (Treg) into tissue is critical in control of inflammation, but has not been examined extensively in chronic graft versus host disease (cGVHD). In parallel studies of tissues and blood, we determined that FoxP3+ T cells increased in proportion to T effectors (Teff) in tissue infiltrates in oral and cutaneous lichenoid cGVHD. These FoxP3+ cells expressed distinguishing phenotypic and functional markers of Treg (CD3+, CD4+, CD27+, ICOS+ and CD39+), not found on FoxP3− Teff. Both Teff and FoxP3+ Treg expressed T-bet and the chemokine receptor CXCR3, however, consistent with a common mechanism of chemokine-mediated migration into tissue. Furthermore, functional markers (ICOS and CD39) and chemokine receptors (CXCR3) were both present in a higher proportion of FoxP3+ cells in tissues than in peripheral blood, consistent with recruitment and activation of Treg in cGVHD target tissues. Finally, the 'activated' CD45RA−FoxP3hi subset of Treg cells, which highly express functional markers, were found in comparable frequencies in cGVHD patients and normal controls, despite a significant deficit in naive 'resting' Treg. These findings are consistent with Treg capacity to upregulate functional markers and traffick into tissue in cGVHD. [ABSTRACT FROM AUTHOR]

Published
2014
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40. Early suppressive effects of chemotherapy and cytokine treatment on committed versus primitive haemopoietic progenitors in patient bone marrow

Author

Schwartz, G. N., primary, Hakim, F., additional, Zujewski, J., additional, Szabo, J. M., additional, Cepada, R., additional, Riseberg, D., additional, Warren, M. K., additional, Mackall, C. L., additional, Setzer, A., additional, Noone, M., additional, Cowan, K. H., additional, O’Shaughnessy, J., additional, and Gress, R. E., additional

Published
1996
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42. Immune dysregulation in TGF-beta 1-deficient mice.

Author

Christ, M, primary, McCartney-Francis, N L, additional, Kulkarni, A B, additional, Ward, J M, additional, Mizel, D E, additional, Mackall, C L, additional, Gress, R E, additional, Hines, K L, additional, Tian, H, additional, and Karlsson, S, additional

Published
1994
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