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302 results on '"Grodsky, G M"'

9. British Diabetic Association Abstracts: Medical and Scientific Section, Autumn Meeting London, 6th and 7th October, 1967

Author

Hill, D. M., Munro-Faure, A. D., Knight, G. J., Anderson, J., Jarrett, R. J., Cohen, N. M., Nelson, J. K., Rabinowitz, D., Merimee, T. J., Baker, P., Mottram, R. F., Moody, A. J., Gliemann, J., Nielsen, Aa. V., Csorba, T. R., Butterfield, W. J. H., Whichelow, M. J., Abrams, M. E., Henderson, J. R., Segall, M. M., Tamir, I., Lloyd, J. K., Hales, C. N., Greenwood, F. C., Mitchell, F. L., Strauss, W. T., Farrant, P. C., Neville, R. W. J., Stewart, G. A., Keen, H., Whittington, T. H., Baird, J. D., Cameron, D., Kellock, M., Scott, A. M., Track, N. S., Watkins, P. J., Smith, J. S., Cohen, H., Hart, A., Jarret, R. J., Malaisse, W. J., Malaisse-Lagae, F., Grodsky, G. M., Bennett, L. L., Turner, D., Howell, S. L., Hellerström, C., Ashcroft, S. J. H., Coll-Garcia, E., Gill, J. B., Randle, P. J., Taylor, K. W., Lacy, P. E., Humbel, R. E., Zahn, H., Danho, W., Schmidt, G., and Falkmer, S.

Published
1968
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10. Functional effects of transgenic expression of cholera toxin in pancreatic beta-cells

Author

Wogensen, L, Ma, Y H, Grodsky, G M, Robertson, R P, Burton, F, Sutcliffe, J G, and Sarvetnick, N

Subjects
Male, Cholera Toxin, Mice, Inbred BALB C, Base Sequence, Recombinant Fusion Proteins, Colforsin, Molecular Sequence Data, Mice, Transgenic, Islets of Langerhans, Mice, Glucose, Phenotype, GTP-Binding Proteins, 1-Methyl-3-isobutylxanthine, Hyperglycemia, Cyclic AMP, Animals, Humans, Insulin, Female, Promoter Regions, Genetic, Signal Transduction
Abstract

Investigation of intracellular pathways of stimulus-secretion signaling in vivo is possible by transgenic expression of agents known to influence specific biochemical interactions in the cells. The objective of the present study was to establish an experimental model for analyzing signal transduction mechanisms in pancreatic beta-cells in vivo, by expressing the cholera toxin A1 subunit under control of the insulin promoter, intending a constant activation of the Gs-protein, and thereby constant generation of cAMP. Surprisingly, the transgenic mice demonstrated mild hyperglycemia and hypoinsulinemia in vivo, and diminished glucose-induced insulin release from the in vitro perfused pancreas, whereas the pancreatic insulin content was normal. These observations suggest a deficiency in either the insulin release mechanisms or glucose recognition. Although the translated cholera toxin A1 subunit was biologically active, there was no increase in the islet content of cAMP. We conclude that the observed phenotype in the cholera toxin transgenic mice may be caused by a deleterious effect of the transgene itself on beta-cell function, or that counter regulatory mechanisms may compensate for the transgene-induced changes in intracellular enzymatic pathways.

Published
1993

46. THE RESPONSE OF HUMAN LEUCOCYTE CULTURES TO STIMULATION BY TUBERCULIN AND PHYTOHAEMAGGLUTININ MEASURED BY THE UPTAKE OF RADIOACTIVE THYMIDINE.

Author

Hartog, M., Cline, M. J., and Grodsky, G. M.

Subjects
MYCOBACTERIUM tuberculosis, THYMIDINE, BACTERIAL antigens, TUBERCULIN, RADIOACTIVE substances, CULTURES (Biology)
Abstract

The article discusses a method for assessing the lymphocyte transformation of human leucocyte cultures induced by phytohaemagglutinin and tuberculin purified protein derivative (PPD) by the measurement of the uptake of thymidine. The thymidine uptake of the cultures has been expressed as percentage of the total activity added. The mean basal uptake of twenty-two unstimulated cultures was 0-96 percent. The mean uptake of twenty-four PHA stimulated cultures was 19-5 percent, whilst the mean uptake of eleven PPD stimulated cultures from seven subjects, who were strongly positive on skin testing to tuberculin, was 20-1 percent. A rise of thymidine uptake was detected in five out of six tuberculin negative subjects.

Published
1967

50. Early phase of insulin release.

Author

Simpson, Ronald G., Benedetti, Andrea, Grodsky, Gerold M., Karam, John H., Forsham, Peter H., Simpson, R G, Benedetti, A, Grodsky, G M, Karam, J H, and Forsham, P H

Published
1968
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