1. New Fe 3 O 4 -Based Coatings with Enhanced Anti-Biofilm Activity for Medical Devices.
- Author
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Pirușcă, Ioana Adelina, Balaure, Paul Cătălin, Grumezescu, Valentina, Irimiciuc, Stefan-Andrei, Oprea, Ovidiu-Cristian, Bîrcă, Alexandra Cătălina, Vasile, Bogdan, Holban, Alina Maria, Voinea, Ionela C., Stan, Miruna S., Trușcă, Roxana, Grumezescu, Alexandru Mihai, and Croitoru, George-Alexandru
- Subjects
SODIUM dodecyl sulfate ,IRON oxides ,PULSED lasers ,CYTOSKELETON ,LACTATE dehydrogenase - Abstract
With the increasing use of invasive, interventional, indwelling, and implanted medical devices, healthcare-associated infections caused by pathogenic biofilms have become a major cause of morbidity and mortality. Herein, we present the fabrication, characterization, and in vitro evaluation of biocompatibility and anti-biofilm properties of new coatings based on Fe
3 O4 nanoparticles (NPs) loaded with usnic acid (UA) and ceftriaxone (CEF). Sodium lauryl sulfate (SLS) was employed as a stabilizer and modulator of the polarity, dispersibility, shape, and anti-biofilm properties of the magnetite nanoparticles. The resulting Fe3 O4 functionalized NPs, namely Fe3 O4 @SLS, Fe3 O4 @SLS/UA, and Fe3 O4 @SLS/CEF, respectively, were prepared by co-precipitation method and fully characterized by XRD, TEM, SAED, SEM, FTIR, and TGA. They were further used to produce nanostructured coatings by matrix-assisted pulsed laser evaporation (MAPLE) technique. The biocompatibility of the coatings was assessed by measuring the cell viability, lactate dehydrogenase release, and nitric oxide level in the culture medium and by evaluating the actin cytoskeleton morphology of murine pre-osteoblasts. All prepared nanostructured coatings exhibited good biocompatibility. Biofilm growth inhibition ability was tested at 24 h and 48 h against Staphylococcus aureus and Pseudomonas aeruginosa as representative models for Gram-positive and Gram-negative bacteria. The coatings demonstrated good biocompatibility, promoting osteoblast adhesion, migration, and growth without significant impact on cell viability or morphology, highlighting their potential for developing safe and effective antibacterial surfaces. [ABSTRACT FROM AUTHOR]- Published
- 2024
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