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2. International Forum on GMP‐grade human platelet lysate for cell propagation: summary

Author

Strunk, D., Lozano, M., Marks, D.C., Loh, Y. S., Gstraunthaler, G., Schennach, H., Rohde, E., Laner‐Plamberger, S., Öller, M., Nystedt, J., Lotfi, R., Rojewski, M., Schrezenmeier, H., Bieback, K., Schäfer, R., Bakchoul, T., Waidmann, M., Jonsdottir‐Buch, S.M., Montazeri, H., Sigurjonsson, O.E., Iudicone, P., Fioravanti, D., Pierelli, L., Introna, M., Capelli, C., Falanga, A., Takanashi, M., Lόpez‐Villar, O., Burnouf, T., Reems, J. A., Pierce, J., Preslar, A.M., and Schallmoser, K.

Published
2018
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4. Guidance Document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)

Author

Pamies, D., Leist, Marcel, Coecke, S., Bowe, G., Allen, D., Gstraunthaler, G., Vries, R.B.M. de, Hartung, Thomas, Stacey, G., Pamies, D., Leist, Marcel, Coecke, S., Bowe, G., Allen, D., Gstraunthaler, G., Vries, R.B.M. de, Hartung, Thomas, and Stacey, G.

Abstract

Contains fulltext : 247184.pdf (Publisher’s version ) (Open Access)

Published
2022

10. International Forum on GMP-grade human platelet lysate for cell propagation

Author

Strunk, D, Lozano, M, Marks, D, Loh, Y, Gstraunthaler, G, Schennach, H, Rohde, E, Laner-Plamberger, S, Öller, M, Nystedt, J, Lotfi, R, Rojewski, M, Schrezenmeier, H, Bieback, K, Schäfer, R, Bakchoul, T, Waidmann, M, Jonsdottir-Buch, S, Montazeri, H, Sigurjonsson, O, Iudicone, P, Fioravanti, D, Pierelli, L, Introna, M, Capelli, C, Falanga, A, Takanashi, M, López-Villar, O, Burnouf, T, Reems, J, Pierce, J, Preslar, A, Schallmoser, K, Strunk D, Lozano M, Marks DC, Loh YS, Gstraunthaler G, Schennach H, Rohde E, Laner-Plamberger S, Öller M, Nystedt J, Lotfi R, Rojewski M, Schrezenmeier H, Bieback K, Schäfer R, Bakchoul T, Waidmann M, Jonsdottir-Buch SM, Montazeri H, Sigurjonsson OE, Iudicone P, Fioravanti D, Pierelli L, Introna M, Capelli C, Falanga A, Takanashi M, López-Villar O, Burnouf T, Reems JA, Pierce J, Preslar AM, Schallmoser K, Strunk, D, Lozano, M, Marks, D, Loh, Y, Gstraunthaler, G, Schennach, H, Rohde, E, Laner-Plamberger, S, Öller, M, Nystedt, J, Lotfi, R, Rojewski, M, Schrezenmeier, H, Bieback, K, Schäfer, R, Bakchoul, T, Waidmann, M, Jonsdottir-Buch, S, Montazeri, H, Sigurjonsson, O, Iudicone, P, Fioravanti, D, Pierelli, L, Introna, M, Capelli, C, Falanga, A, Takanashi, M, López-Villar, O, Burnouf, T, Reems, J, Pierce, J, Preslar, A, Schallmoser, K, Strunk D, Lozano M, Marks DC, Loh YS, Gstraunthaler G, Schennach H, Rohde E, Laner-Plamberger S, Öller M, Nystedt J, Lotfi R, Rojewski M, Schrezenmeier H, Bieback K, Schäfer R, Bakchoul T, Waidmann M, Jonsdottir-Buch SM, Montazeri H, Sigurjonsson OE, Iudicone P, Fioravanti D, Pierelli L, Introna M, Capelli C, Falanga A, Takanashi M, López-Villar O, Burnouf T, Reems JA, Pierce J, Preslar AM, and Schallmoser K

Published
2018

11. Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0) - Draft for stakeholder discussion and call for action

Author

Pamies, D., Leist, M., Coecke, S., Bowe, G., Allen, D., Gstraunthaler, G., Bal-Price, A., Pistollato, F., Vries, R.B. de, Hartung, T., Stacey, G., Pamies, D., Leist, M., Coecke, S., Bowe, G., Allen, D., Gstraunthaler, G., Bal-Price, A., Pistollato, F., Vries, R.B. de, Hartung, T., and Stacey, G.

Abstract

Contains fulltext : 225450.pdf (publisher's version ) (Open Access)

Published
2020

17. International Forum on GMP-grade human platelet lysate for cell propagation: summary

Author

Strunk, D., primary, Lozano, M., additional, Marks, D.C., additional, Loh, Y. S., additional, Gstraunthaler, G., additional, Schennach, H., additional, Rohde, E., additional, Laner-Plamberger, S., additional, Öller, M., additional, Nystedt, J., additional, Lotfi, R., additional, Rojewski, M., additional, Schrezenmeier, H., additional, Bieback, K., additional, Schäfer, R., additional, Bakchoul, T., additional, Waidmann, M., additional, Jonsdottir-Buch, S.M., additional, Montazeri, H., additional, Sigurjonsson, O.E., additional, Iudicone, P., additional, Fioravanti, D., additional, Pierelli, L., additional, Introna, M., additional, Capelli, C., additional, Falanga, A., additional, Takanashi, M., additional, Lόpez-Villar, O., additional, Burnouf, T., additional, Reems, J. A., additional, Pierce, J., additional, Preslar, A.M., additional, and Schallmoser, K., additional

Published
2017
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18. Fetal Bovine Serum (FBS) — A Pain in the Dish?

Author

van der Valk, J.B.F., Gstraunthaler, G., van der Valk, J.B.F., and Gstraunthaler, G.

Abstract

The use of Fetal Bovine Serum in replacement alternative methods is associated with serious animal welfare concerns, as well as worrying reproducibility issues

Published
2017

23. A plea to reduce or replace fetal bovine serum in cell culture media

Author

Gstraunthaler, G., Lindl, T., van der Valk, J.B.F., dASS BW-1, Dep of Animals in Science and Society, LAS/3’R Centre ULS, dASS BW-1, Dep of Animals in Science and Society, and LAS/3’R Centre ULS

Subjects
business.industry, Clinical Biochemistry, Biomedical Engineering, Bioengineering, Cell Biology, Biology, Bioinformatics, Biotechnology, Supply and demand, Biosafety, Prion Proteins, business, Letter to the Editor, Fetal bovine serum
Abstract

To the Editor,Fetal bovine serum (FBS) is a universal growthsupplement of cell and tissue culture media. FBS is anatural cocktail of most of the factors required for cellattachment, growth, and proliferation, effective formost types of human and animal (including insect)cells. Although in use for more than 50 years, FBS hasnever been fully characterized. Recent proteomic andmetabolomic studies revealed approx. 1,800 proteins(Anderson and Anderson 2002; Anderson et al. 2004)and more than 4,000 metabolites (Psychogios et al.2011) present in serum. However, the use of serum incell culture also bears a number of disadvantages.These disadvantages can either be seen from: (a) ascientific, cell biological point of view, since serum ingeneral is an ill-defined mixture of components inculture media, with qualitative and quantitative,geographical and seasonal batch-to-batch variations,(b) from biosafety aspects, since FBS may containadverse factors, like endotoxin, mycoplasma, viralcontaminants or prion proteins, (c) from ethicalperspectives in terms of animal protection argumentsregarding the harvest and collection of FBS frombovine fetuses, and (d) in terms of recent concernsabout the global supply versus demand of FBS. As aconsequence, a number of strategies were developedto reduce or replace the requirement for FBS in cellculture media.FBS is a by-product of the beef packing industry.Thus, the supply is dictated by many factors, includingbeef consumption, dairy product consumption, feedprices, environmental factors such as drought, cattleimport and export, governmental farm policies (Sha-iler and Corrin 1999), and the outbreak of diseases(foot and mouth disease, BSE) (Asher 1999; Dormont1999; Even et al. 2006; Wessmann and Levings ).The availability of FBS has changed dramatically overthe past few years (Fujimoto 2002). Therefore, allefforts and attemptsshouldbe undertaken toovercomethe expected shortfall in FBS supply. In the case ofFBS, supply versus demand models do not followtypical economic principles. Normally, supply can beadjusted to meet the demand. However, in case ofFBS, supply and demand operate independently ofeach other. In addition, there is a severe geographicalmismatch between the supply of and the demand forFBS. Demand is highest in US and Europe, while themajor sources of FBS are far away—in Brasil,Argentina, South Africa, Australia, New Zealand,and Central America, since in those countries huge

Published
2013

28. A plea to reduce or replace fetal bovine serum in cell culture media

Author

dASS BW-1, Dep of Animals in Science and Society, LAS/3’R Centre ULS, Gstraunthaler, G., Lindl, T., van der Valk, J.B.F., dASS BW-1, Dep of Animals in Science and Society, LAS/3’R Centre ULS, Gstraunthaler, G., Lindl, T., and van der Valk, J.B.F.

Published
2013

29. Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Author

Algemeen Onderzoek DGK, Dep of Animals in Science and Society, van der Valk, J.B.F., Brunner, D., De Smet, K., Fex Svenningsen, Å., Honegger, P., Knudsen, L.E., Lindl, T., Noraberg, J., Price, A., Scarino, M.L., Gstraunthaler, G., Algemeen Onderzoek DGK, Dep of Animals in Science and Society, van der Valk, J.B.F., Brunner, D., De Smet, K., Fex Svenningsen, Å., Honegger, P., Knudsen, L.E., Lindl, T., Noraberg, J., Price, A., Scarino, M.L., and Gstraunthaler, G.

Published
2010

30. Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods

Author

van der Valk, J, Brunner, D, De Smet, K, Fex Svenningsen, A, Honegger, P, Knudsen, Lisbeth E., Lindl, T, Noraberg, J, Price, Anna, Scarino, M L, Gstraunthaler, G, van der Valk, J, Brunner, D, De Smet, K, Fex Svenningsen, A, Honegger, P, Knudsen, Lisbeth E., Lindl, T, Noraberg, J, Price, Anna, Scarino, M L, and Gstraunthaler, G

Abstract

Udgivelsesdato: 2010-Jun, Quality assurance is becoming increasingly important. Good laboratory practice (GLP) and good manufacturing practice (GMP) are now established standards. The biomedical field aims at an increasing reliance on the use of in vitro methods. Cell and tissue culture methods are generally fast, cheap, reproducible and reduce the use of experimental animals. Good cell culture practice (GCCP) is an attempt to develop a common standard for in vitro methods. The implementation of the use of chemically defined media is part of the GCCP. This will decrease the dependence on animal serum, a supplement with an undefined and variable composition. Defined media supplements are commercially available for some cell types. However, information on the formulation by the companies is often limited and such supplements can therefore not be regarded as completely defined. The development of defined media is difficult and often takes place in isolation. A workshop was organised in 2009 in Copenhagen to discuss strategies to improve the development and use of serum-free defined media. In this report, the results from the meeting are discussed and the formulation of a basic serum-free medium is suggested. Furthermore, recommendations are provided to improve information exchange on newly developed serum-free media.

Published
2010

31. The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture

Author

Universiteit Utrecht, Faculteit Diergeneeskunde, van der Valk, J.B.F., Mellor, D., Brands, R., Fischer, R., Gruber, F., Gstraunthaler, G., Hellebrekers, L.J., Hyllner, J., Jonker, F.H., Prieto, P., Thalen, M., Baumans, V., Universiteit Utrecht, Faculteit Diergeneeskunde, van der Valk, J.B.F., Mellor, D., Brands, R., Fischer, R., Gruber, F., Gstraunthaler, G., Hellebrekers, L.J., Hyllner, J., Jonker, F.H., Prieto, P., Thalen, M., and Baumans, V.

Published
2004

47. Fusion of cultured dog kidney (MDCK) cells: I. Technique, fate of plasma membranes and of cell nuclei.

Author

Kersting, U, Joha, H, Steigner, W, Gassner, B, Gstraunthaler, G, Pfaller, W, and Oberleithner, H

Abstract

The evaluation of the intracellular signal train and its regulatory function in controlling transepithelial transport with electrophysiological methods often requires intracellular measurements with microelectrodes. However, multiple impalements in epithelial cells are hampered by the small size of the cells. In an attempt to avoid these problems we fused cells of an established cell line. Madin Darby canine kidney cells, originally derived from dog kidney, to "giant" cells by applying a modified polyethylene glycol method. During trypsin-induced detachment from the ground of the petri dish, individual cells grown in a monolayer incorporate volume and mainly lose basolateral plasma membrane by extrusion. By isovolumetric cell-to-cell fusion, spherical "giant" cells are formed within 2 hr. During this process a major part of the individual cell plasma membranes is internalized. Over three weeks following cell plasma membrane fusion degradation of single cell nuclei and cell nuclear fusion occurs. We conclude that this experimental approach opens the possibility to investigate ion transport of epithelia in culture by somatic cell genetic techniques. [ABSTRACT FROM AUTHOR]

Published
1989

50. Precursors of ribose 5-phosphate suppress expression of glucose-regulated proteins in LLC-PK1 cells

Author

Gstraunthaler, G., Harris, H. W., and Handler, J. S.

Abstract

Withdrawal of glucose from the medium bathing mammalian cells in culture results in cessation of growth and induces the synthesis of two stress proteins (Mr approximately 94-100 kDa and 78-80 kDa) that have been termed glucose-regulated proteins (GRPs). In LLC-PK1 cells, proteins of the same molecular weights, assayed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography, are fully induced by 24 h in glucose-free medium. The GRPs from LLC-PK1 cells cross-react with antibodies to GRPs of nonpolar cells, confirming their identification. Since glucose is not essential for energy production in cultured cells, but is essential for ribose 5-phosphate and nucleotide biosynthesis (Wice et al. J. Biol. Chem. 256: 7812-7819, 1981), we tested the effect of several precursors of ribose 5-phosphate on the induction of GRPs. The addition of 25 mM fructose or galactose to glucose-free medium suppressed the induction of GRPs. The pyrimidine ribonucleosides uridine and cytidine also suppressed GRP synthesis, ribose and the purine ribonucleosides guanosine and adenosine suppressed partially. The results, coupled with indirect evidence in the literature, lead to the suggestion that cell ribose 5-phosphate or a related metabolite regulates the expression of GRPs.

Published
1987
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