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Understanding cellular architectures and their connectivity is essential for interrogating system function and dysfunction. However, we lack technologies for mapping the multiscale details of individual cells and their connectivity in the human organ-scale system. We developed a platform that simultaneously extracts spatial, molecular, morphological, and connectivity information of individual cells from the same human brain. The platform includes three core elements: a vibrating microtome for ultraprecision slicing of large-scale tissues without losing cellular connectivity (MEGAtome), a polymer hydrogel-based tissue processing technology for multiplexed multiscale imaging of human organ-scale tissues (mELAST), and a computational pipeline for reconstructing three-dimensional connectivity across multiple brain slabs (UNSLICE). We applied this platform for analyzing human Alzheimers disease pathology at multiple scales and demonstrating scalable neural connectivity mapping in the human brain.

Background: The rising incidence of hilar malignant bile duct strictures poses challenges for diagnosis and treatment. While endoscopic treatment is essential for relieving obstruction, it carries a high risk of postoperative cholangitis. Sequential cutting of nasobiliary tubes as an alternative to stent placement after nasobiliary drainage may emerge as an effective strategy to mitigate these complications. Objectives: This study aimed to evaluate the efficacy and safety of nasobiliary tube cutting after nasobiliary drainage versus conventional stent placement in reducing postoperative cholangitis in patients with hilar malignant biliary strictures. Design: A retrospective cohort study. Methods: From 2015 to 2023, 208 patients were divided into two groups: nasobiliary tube cutting group (n = 103) and conventional stent group (n = 105). The primary outcome was postoperative cholangitis, with secondary outcomes including drainage success, bilirubin reduction, re-interventions, complications, and hospital stay. Results: Cholangitis occurred in 11.7% of the nasobiliary tube cutting group and 26.7% of the conventional stent group (p = 0.006). Successful drainage was achieved in 84 patients (81.6%) in the nasobiliary tube cutting group and 78 patients (74.3%) in the conventional stent group, with no statistically significant difference (p = 0.207). Patients whose total bilirubin reduction >50% within 7 days were significantly higher in the nasobiliary tube cutting group (48.5% vs 27.6%, p = 0.002). There were no significant differences between the groups in the number of endoscopic retrograde cholangiopancreatography procedures or re-interventions required (p > 0.05). Except for postoperative cholangitis, other postoperative complications were comparable between the groups (p > 0.05). The postoperative hospital stay was significantly longer in the nasobiliary tube cutting group, with a median duration of 10.0 (7.0, 14.0) days compared to 7.0 (5.5, 12.5) days in the conventional stent group (p = 0.024). Bismuth-Corlette Type IV was identified as an independent risk factor for cholangitis (OR = 3.207, 95%CI: 1.253–8.210, p = 0.015). Conclusion: For patients with hilar malignant biliary stricture, sequential cutting of nasobiliary tubes as an alternative to stent placement after nasobiliary drainage may reduce the incidence of postoperative cholangitis and achieve early successful drainage, but it may result in a prolonged postoperative hospital stay. Bismuth-Corlette Type IV is an independent risk factor for the development of postoperative cholangitis. [ABSTRACT FROM AUTHOR]

The discovery of high critical temperature in cubic H3S under high pressures attracts significant attention. Of particular recent interest is to search for superconductors stabilized at low pressure in order to approach practical applications. To examine the doping effect on the stability and superconductivity of H3S, we construct H6SX by replacing half of the S atoms with an X atom (X = Si, Ge, Sn, P, As, Sb, Te, Cl, Br and I). In addition to previously reported compounds, we identified seven new superconductors that are dynamically stable below 200 GPa, e.g., Fd3¯m H6SAs, H6SSb, H6SI, H6SSn, H6STe, Cmmm H6SCl, H6SBr. Among these, H6SSb exhibits Tc of 91 K at 200 GPa, and increases to 115 K as pressure decreases to 40 GPa. Electron–phonon analysis indicates that the coupling between the electron of Sb atoms and the associated low-frequency phonons is responsible for the enhancement of the superconductivity. Additionally, the superconductivity of H6SSX is closely related to the electronegativity of X in the same main group, where X with a larger electronegativity results in a strong H–X bond, resulting in a higher superconductivity. [ABSTRACT FROM AUTHOR]

Peripheral nerve injury may result in muscle atrophy and impaired motor function recovery, and numerous pieces of evidence indicate that long noncoding RNAs (lncRNAs) play crucial roles in skeletal muscle regeneration. Our preliminary sequencing results showed that LNC_000280 was significantly down-regulated in denervated mouse skeletal muscle and we hypothesized that LNC_000280 may play an important role in skeletal muscle regeneration. In this research, flow cytometry and EdU staining results showed that overexpression of LNC_000280 promoted the proliferation of C2C12, while knockdown LNC_000280 had the opposite effect. Knockdown LNC_000280 inhibited the differentiation of C2C12 cells. LNC_000280 regulated the expression of proliferation genes (Cdk2, Cdc27) and differentiation genes (MyoG, MyoD). GO analysis and PPI network of LNC_000280 target genes showed that LNC_000280 mainly regulates skeletal muscle cell metabolism, mitochondrial and muscle growth. Idh2, Klhl31, Agt, and Gpt2 may be important downstream targets for its function. Therefore, we believe that that LNC_000280 can regulate the proliferation and differentiation of myoblasts by regulating gene expression. [ABSTRACT FROM AUTHOR]

Ratoon rice occupies an important position in rice production owing to its time-saving, labor-saving and low-pollution planting, and increased benefits. However, the impact of tillage management on the yield in rice ratooning system has not yet been reported. Thus, field experiments were carried out to investigate the impact of seven tillage methods on the yield of ratoon rice crop in Jingzhou City in 2021–2022. The managements included winter plowing + rotary 2 times (PTw + RT2) or 3 times (PTw + RT3), spring plowing + rotary 2 times (PTs + RT2) or 3 times (PTs + RT3), no plowing + rotary 2 times (P0 + RT2) or 3 times (P0 + RT3) and no tillage (NT). PTw + RT3 had the highest total rice yield. The experimental data were collected in 2021 and 2022. In terms of main season rice yield, the order of ranking was PTw > PTs ≈ NT ≈ P0, while for ratoon rice yield, the ranking was NT > PTw ≈ PTs > P0. Generally, the root function ranked as PTw > PTs > P0 > NT. The photosynthetic capacity of the main season rice always maximized in PTw, those of the ratoon rice all maximized in NT, and those of both the main season rice and ratoon rice always minimized in P0. In the three tillage modes (PTw, PTs, P0), an additional rotary tillage did not affect the growth or yield of rice. PTw + 3RT was the highest yielding tillage management, but it is still necessary to explore other PTw + 3RT methods and more economical tillage management to increase the yield of ratoon rice. [ABSTRACT FROM AUTHOR]