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24 results on '"Gu, Zhenning"'

3. TMPO-AS1, a Novel E2F1-Regulated lncRNA, Contributes to the Proliferation of Lung Adenocarcinoma Cells via Modulating miR-326/SOX12 Axis

Author

Wei,Lin, Liu,Yugang, Zhang,Huijun, Ma,Yuefeng, Lu,Zenghui, Gu,Zhenning, and Ding,Chao

Subjects
Cancer Management and Research
Abstract

Lin Wei,1 Yugang Liu,1 Huijun Zhang,1 Yuefeng Ma,2 Zenghui Lu,1 Zhenning Gu,1 Chao Ding1 1Department of Thoracic Surgery, Xi’an Chest Hospital, Xi’an 710100, People’s Republic of China; 2Department of Thoracic Surgery, The Second Affiliated Hospital of Xi’an Jiao Tong University, Xi’an 710004, People’s Republic of ChinaCorrespondence: Chao DingDepartment of Thoracic Surgery, Xi’an Chest Hospital, Xi’an, Shaanxi Province 710100, People’s Republic of ChinaTel/Fax +86-29-62500053Email abroad2014@sohu.comBackground: TMPO-AS1, an antisense lncRNA located at human chromosome 12p23.1, has been identified as an oncogene involved in cell proliferation in various cancers, including LUAD. In this study, we aimed to explore the novel molecular mechanism of TMPO-AS1 underlying LUAD growth.Materials and Methods: The transcription levels of TMPO-AS1, miR-326, and SOX12 in LUAD tissues and cell lines were detected by quantitative real-time PCR (qRT-PCR). The cell proliferation ability was evaluatect 3d by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis analysis was assessed by flow cytometry. The target relationship among TMPO-AS1, miR-326, and SOX12 and promoter activity of TMPO-AS1 was measured using dual-luciferase reporter assay. The protein levels of SOX12 in LUAD cells were determined by Western blot. ChIP-qPCR assay was performed to validate the direct binding between E2F1 and TMPO-AS1 promoter.Results: TMPO-AS1 was up-regulated in LUAD tissues as well as cell lines. Boosted TMPO-AS1 expression was positively correlated with poor prognosis and pathological stage in LUAD. Down-regulation of TMPO-AS1 could restrain the proliferation of LUAD cells through arresting the cell cycle at G0/G1 phase and inducing apoptosis in vitro. Mechanically, we demonstrated that TMPO-AS1 could modulate the proliferation of LUAD cells through increasing SOX12 expression level via sponging miR-326 in accordance with bioinformatics analysis and experimental validation. Furthermore, we identified that TMPO-AS1 could be activated by E2F transcription factor 1 (E2F1) as a novel target gene.Conclusion: TMPO-AS1 can modulate LUAD cell proliferation through E2F1/miR-326/SOX12 pathway.Keywords: lung adenocarcinoma, TMPO-AS1, cell proliferation, miR-326, SOX12, E2F1

Published
2020

4. Bulk Cutting of Carbon Nanotubes Using Electron Beam Irradiation

Author

Ziegler, Kirk J, Rauwald, Urs, Hauge, Robert H, Schmidt, Howard K, Smalley, Richard E, Kittrell, W. Carter, and Gu, Zhenning

Subjects
Composite Materials
Abstract

According to some embodiments, the present invention provides a method for attaining short carbon nanotubes utilizing electron beam irradiation, for example, of a carbon nanotube sample. The sample may be pretreated, for example by oxonation. The pretreatment may introduce defects to the sidewalls of the nanotubes. The method is shown to produces nanotubes with a distribution of lengths, with the majority of lengths shorter than 100 tun. Further, the median length of the nanotubes is between about 20 nm and about 100 nm.

Published
2013

5. Identification of large fullerenes formed during the growth of single-walled carbon nanotubes in the HiPco process

Author

Ramesh, Sivarajan, Brinson, Bruce, Margrave, J.L., Johnson, M. Pontier, Gu, Zhenning, Saini, Rajesh K., Willis, Peter, Marriott, Terry, Billups, W.E., Hauge, Robert H., and Smalley, Richard E.

Subjects
Mass spectrometry -- Usage, Nanotubes -- Chemical properties, Chemicals, plastics and rubber industries
Abstract

A small fraction of high-molecular-weight closed shell carbon clusters are formed along with the single-walled carbon nanotubes during the catalytic, high-pressure decomposition of carbon monoxide. The fullerenic clusters and can be desorbed by laser irradiation thus providing for a quantitative estimation by LDI-TOF mass spectrometry with appropriate markers.

Published
2003

14. Fluorination of pressure-polymerized C60 phases

Author

Gu, Zhenning, primary, Zhang, Lei, additional, Margrave, John L., additional, Davydov, Valery A., additional, Rakhmanina, Aleksandra V., additional, Agafonov, Vyacheslav, additional, and Khabashesku, Valery N., additional

Published
2005
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15. Cutting single-walled carbon nanotubes

Author

Ziegler, Kirk J, primary, Gu, Zhenning, additional, Shaver, Jonah, additional, Chen, Zheyi, additional, Flor, Erica L, additional, Schmidt, Daniel J, additional, Chan, Candace, additional, Hauge, Robert H, additional, and Smalley, Richard E, additional

Published
2005
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24. Fluorination of pressure-polymerized C60 phases

Author

Gu, Zhenning, Zhang, Lei, Margrave, John L., Davydov, Valery A., Rakhmanina, Aleksandra V., Agafonov, Vyacheslav, and Khabashesku, Valery N.

Subjects
*REACTIVITY (Chemistry), *FLUORINE, *TEMPERATURE, *FOURIER transform infrared spectroscopy, *X-ray diffraction, *STOICHIOMETRY, *POLYMERS, *FLUOROPOLYMERS
Abstract

Abstract: The reactivity of O-, T- and R-phases of the high pressure–high temperature (HPHT) polymerized C60 towards gaseous fluorine in the temperature range of 50–250°C was investigated. The reaction products were characterized by FTIR, powder X-ray diffraction, SEM, EDX, and VTP-EIMS to determine the bulk stoichiometries, bonding patterns, phase compositions, crystalline structures and thermal decomposition behavior of the fluorinated polymers. At 1 h isothermal treatment duration, fluorinated products with various bulk stoichiometries were obtained from different polymer phases with the R-phase showing the highest fluorine uptake. At 250°C, all C60 polymers showed partial decomposition to unfluorinated C60 monomer under fluorine atmosphere. At 200°C, the fluorination of R-phase yielded a pure fluoropolymer most likely having a {C60F x } n (x =36–44) composition. The same fluoropolymer was presumably obtained from O- and T-phases in lower yields. The linear chain structure was suggested for this new fluorocarbon polymer in agreement with the molecular mechanics modeling calculations. [Copyright &y& Elsevier]

Published
2005
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