Search

Los aceites esenciales se caracterizan por presentar olor, sabor y muchas veces propiedades antifúngicas;son producidos por plantas aromáticas y medicinales. Es importante determinar qué efecto tienen los aceites esenciales de L graveolensen el control de fitopatógenos. Se realizó la evaluación del aceite esencial de Lippia graveolens sobre el hongo Fusarium chlamydosporum, que tiene alta incidencia, reduce la productividad y puede afectar al humano. La extracción de los aceites se realizó en etanol absoluto. De la biomasa se realizó la extracción de DNA y se hizo una PCR utilizando los oligonucleótidos ITS1 e ITS4. El hongose confrontóen cajas de petri con los aceites esencialesde L graveolens. Seobservó menor crecimiento de Fusarium sp, en la muestra Viad 01, con 1.23 cm de crecimiento;mientras que en el testigo se obtuvo una colonia de 4.60 cm, lo cual representa una diferencia de 3.37 cm con respecto a la muestra Viad 01. Lo anterior muestra que los extractos de Lippia graveolenspueden ser una alternativa para el control de enfermedades innovando para la agricultura sostenible y competitiva, además de su uso potencial en salud humana.

Background: Human breast milk is a complex fluid that provides both macronutrients and micronutrients critical for the infant development. Several studies have been focused on the characterization of the human breast milk (HBM) microbiota, nowadays we know it is composed by a rich community of bacteria, viruses, protozoa and anaerobic fungi. However, it is considered that the human microbiome is not static, instead is described as a dynamic living system shaped by several factors like age, demography, malnutrition, sex, diet, sexual behavior, antibiotics and health condition. Previous studies have demonstrated that metabolic disorders such as diabetes mellitus induces dysbiosis in the gut microbiome in affected individuals. However, how gestational diabetes mellitus (GDM) affects the HBM microbiota remains poorly understood. In this study, we analyzed the bacterial diversity through the massive sequencing of the 16S rRNA in Colombian infants breastfed with HBM from GDM and control women. Methods: In this descriptive cross-sectional study, we collected HBM and stool samples from GDM and healthy mother-infant pairs in Pereira (Colombia). The whole DNA was purified and the 16S V3-V4 region was amplified and sequenced. Reads obtained were quality filtered and classified by homology according to the Ribosomal Small Subunit SILVA. Results: Our results revealed that the most abundant phyla in the collected samples are Firmicutes, Bacteroidetes and Proteobacteria. Interestingly, we found significant differences in the bacterial relative abundances GDM mothers’ gut compared to the control group, notably Bifidobacterium, Serratia and Sutterella were negatively associated to GDM. In the HBM significant differences were observed in Sutterella, Serratia, Lactococcuswere in low RA in GDM whereas Veillonellawas in high RA. On the other hand, in the infant, Bifidobacterium, Lactobacillus, Sutterella, Serratia, Streptococcus, and Veillonella had low presence while Lactococcus and Flavonifractor were in significant high abundance in GDM, these differences in the bacterial community structure in GDM mother and their infants could be linked to vitamin K and several forms of vitamin B deficiency, inflammatory disease, and gut bacterial homeostasis. Conclusion: Our results suggest that gut bacteriome profiles vary between healthy and GDM women, leading to gut bacterial dysbiosis in their infants.

Objetivo: Se evaluó la actividad antimicrobiana de un extracto crudo de B. bassiana y dos fracciones del mismo contra bacterias de importancia clínica. Métodos: El micelio de cepa B. bassiana se remojó en metanol durante una semana, después se evaporo en un rotovapor a 45°C aplicando vacío. El extracto metanólico se hizo pasar con dos fases móviles para obtener una fracción A y B. La fracciones A, B y el extracto crudo C se evaluaron contra las cepas Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella tiphy, Pseudomonas aeruginosa y Acinetobacter baumannii con la técnica de microdilución en placa. Resultados: En la fracción A se observó un efecto antimicrobiano contra Salmonella typhi, Pseudomonas aeruginosa y Acinetobacter baumannii el crecimiento bacteriano alcanzó el 70, 60 y 83 % respectivamente. La fracción B causó un efecto antimicrobiano en Klebsiella pneumoniae, S. typhi, P. aeruginosa y A. baumannii con un crecimiento bacteriano del 62, 58, 41 y 7 % respectivamente. Y El extracto crudo no causó inhibición del crecimiento en A. baumannii, pero para el resto de la bacterias hubo un crecimiento del 56 al 88 %. Conclusiones: Beauveria bassiana es un hongo entomopatógeno que produce diferentes metabolitos con actividad insecticida, citotóxica, antifúngica, antibiótica y antiviral. Este es el primer estudio de los efectos antimicrobianos de un extracto metanólico del hongo entomopatógeno B. bassiana contra cepas bacterianas de importancia clínica., Objective: The antimicrobial activity of a crude extract of B. bassiana and two fractions thereof against clinically important bacteria was evaluated. Methods: The mycelium of strain B. bassiana was soaked in methanol for a week, then it was evaporated in a rotovap at 45 ° C applying a vacuum. The methanolic extract was passed through two mobile phases to obtain a fraction A and B. Fractions A, B and crude extract C were evaluated against the strains Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella tiphy, Pseudomonas aeruginosa and Acinetobacter baumannii with the plate microdilution technique. Results: In fraction A an antimicrobial effect against Salmonella typhi, Pseudomonas aeruginosa and Acinetobacter baumannii was observed, bacterial growth reached 70, 60 and 83% respectively. Fraction B caused an antimicrobial effect in Klebsiella pneumoniae, S. typhi, P. aeruginosa and A. baumannii with a bacterial growth of 62, 58, 41 and 7% respectively. And the crude extract did not cause growth inhibition in A. baumannii, but for the rest of the bacteria there was a growth of 56 to 88%. Conclusions: Beauveria bassiana is an entomopathogenic fungus that produces different metabolites with insecticidal, cytotoxic, antifungal, antibiotic and antiviral activity. This is the first study of the antimicrobial effects of a methanolic extract of the entomopathogenic fungus B. bassiana against bacterial strains of clinical importance.