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2. Caloric restriction leading to attenuation of experimental Alzheimer's disease results from alterations in gut microbiome.

Author

Chen, Junyu, Zou, Cong, Guan, Hongbing, Zhou, Xiaoming, Hou, Le, Cui, Yayong, Xu, Junhua, Luan, Ping, and Zheng, Dong

Abstract

Background: Caloric restriction (CR) might be effective for alleviating/preventing Alzheimer's disease (AD), but the biological mechanisms remain unclear. In the current study, we explored whether CR caused an alteration of gut microbiome and resulted in the attenuation of cognitive impairment of AD animal model. Methods: Thirty‐week‐old male APP/PS1 transgenic mice were used as AD models (AD mouse). CR was achieved by 30% reduction of daily free feeding (ad libitum, AL) amount. The mice were fed with CR protocol or AL protocol for six consecutive weeks. Results: We found that with CR treatment, AD mice showed improved ability of learning and spatial memory, and lower levels of Aβ40, Aβ42, IL‐1β, TNF‐α, and ROS in the brain. By sequencing 16S rDNA, we found that CR treatment resulted in significant diversity in composition and abundance of gut flora. At the phylum level, Deferribacteres (0.04%), Patescibacteria (0.14%), Tenericutes (0.03%), and Verrucomicrobia (0.5%) were significantly decreased in CR‐treated AD mice; at the genus level, Dubosiella (10.04%), Faecalibaculum (0.04%), and Coriobacteriaceae UCG‐002 (0.01%) were significantly increased in CR‐treated AD mice by comparing with AL diet. Conclusions: Our results demonstrate that the attenuation of AD following CR treatment in APP/PS1 mice may result from alterations in the gut microbiome. Thus, gut flora could be a new target for AD prevention and therapy. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Gradient sequential treatment of temporomandibular joint disorders

Author

ZHANG Qingbin and GUAN Hongbing

Subjects
temporomandibular joint disorders，, abnormal mandibular movement ，, regional pain around the temporomandibular joint，, clicking noises around the temporomandibular joint，, gradient sequential treatment，, physical therapy，, occlusal plate treatment ，, injection therapy，, articular lavage，, arthroscopic minimally invasive surgery，, open surgery，, personalized，, Medicine
Abstract

Temporomandibular joint disorder (TMD) is a common clinical disease in stomatology that occurs frequently in young people, mostly in women, with an incidence of approximately 30%; its clinical manifestations include mandibular dysfunction, regional pain, and clicking noises around the temporomandibular joint (TMJ). Some patients have tinnitus, headache and other symptoms. With regard to the treatment procedures of TMD, a gradient sequential treatment model is currently preferred, each of which has strict indications. Generally, conservative treatment or noninvasive treatment is preferred and is suitable for patients with dysfunction or mild organic disease. The second-choice minimally invasive treatment is suitable for patients who have failed conservative treatment or patients with mild organic disease. Finally, open surgery, which is suitable for patients who are not responsive to the first two treatments and show severe organic lesions, can be considered. The formulation of an open surgery treatment diagnosis and treatment plan should be personalized, led by doctors, and completed with the cooperation of patients. This article describes the “gradient sequential treatment” of temporomandibular joint disorders.

Published
2020
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4. Research on the inhibitory effect of Baicalin on the human tongue squamous cell carcinoma cell line SCC15

Author

ZHONG Wende, LIU Rongjing, GUAN Hongbing, and CHEN Guangsheng

Subjects
Baicalin, Tongue, Carcinoma, Squamous cell, SCC15 cell, Migration, Cell cycle, Signal transduction and transcription activator 3(STAT3), Medicine
Abstract

Objective To explore the inhibitory effect and possible mechanism of Baicalin on the human tongue squamous cell carcinoma cell line SCC15 and to provide a new idea and experimental basis for the clinical prevention and treatment of tongue squamous cell carcinoma. Methods SCC15 cells cultured in DMEM alone were used as the control group, and SCC15 cells cultured in 20 mg/mL baicalin solution were used as the baicalin group. Scratch tests and Transwell migration tests were performed to detect changes in cell migration ability, and flow cytometry was used to detect changes in the cell cycle. Western blotting was used to detect differences in the phosphorylation levels of signal transduction and transcription activator 3 (STAT3). Results Compared with the control group, the scratch test and the Transwell migration test showed that the cell migration ability of cells in the baicalin group was significantly decreased (t＝4.927, P＝0.008); flow cytometry showed that the number of cells of the baicalin group increased in the G0/G1 phase (t＝9.893, P＝0.001), decreased in the S phase (t＝8.528, P＝0.001), and decreased in the G2/M phase (t＝ 3.550, P＝0.024); Western blotting results showed that the STAT3 protein of SCC15 cells in the baicalin group de⁃creased (t＝3.550, P＝0.024), and the phosphorylation level significantly decreased (t＝8.262, P＝0.001). Conclu⁃ sion Baicalin inhibits the human tongue squamous cell carcinoma cell line SCC15, and its mechanism may be related to a decrease in STAT3 pathway phosphorylation activity.

Published
2019
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12. The current understanding of immunotoxicity induced by silica nanoparticles

Author

Shao Longquan, Zhong Wenchao, Liu Jia, Guan Hongbing, Kang Yiyuan, Chen Liangjiao, and Zhang Yanli

Subjects
Chemistry, Autophagy, Biomedical Engineering, Medicine (miscellaneous), Bioengineering, Development, Silicon Dioxide, Silica nanoparticles, Immune system, Biophysics, Animals, Humans, Nanoparticles, General Materials Science, Current (fluid), Cytotoxicity
Published
2019

23. Blockade of the NLRP3/Caspase-1 Axis Ameliorates Airway Neutrophilic Inflammation in a Toluene Diisocyanate-Induced Murine Asthma Model

Author

Chen, Shuyu, primary, Yao, Lihong, additional, Huang, Peikai, additional, He, Qiaoling, additional, Guan, Hongbing, additional, Luo, Yiqin, additional, Zou, Zehong, additional, Wei, Shushan, additional, Peng, Guoyou, additional, Yan, Jie, additional, Chen, Rongchang, additional, Zhang, Qingling, additional, and Tao, Ailin, additional

Published
2019
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24. CD44 deletion leading to attenuation of experimental autoimmune encephalomyelitis results from alterations in gut microbiome and short-chain fatty acids

Author

Chitrala, Kumaraswamy Naidu, Guan, Hongbing, Singh, Narendra P., Busbee, Brandon, Gandy, Alexa, Mehrpouya-Bahrami, Pegah, Ganewatta, Mitra S., Tang, Chuanbing, Chatterjee, Saurabh, Nagarkatti, Prakash, and Nagarkatti, Mitzi

Subjects
Mice, Knockout, Encephalomyelitis, Autoimmune, Experimental, Bacteroidetes, Firmicutes, Fecal Microbiota Transplantation, Fatty Acids, Volatile, Article, Gastrointestinal Microbiome, Mice, Inbred C57BL, Disease Models, Animal, Feces, Mice, Hyaluronan Receptors, RNA, Ribosomal, 16S, Animals, Dysbiosis, Metagenomics, Propionates, Gene Deletion
Abstract

Dysbiosis in gut microbiome has been shown to be associated with inflammatory and autoimmune diseases. Previous studies from our laboratory demonstrated the pivotal role played by CD44 in the regulation of experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS). Specifically, we showed that CD44 knockout (KO) mice were resistant to induction of EAE when compared to CD44wildtype (WT) mice and this was due to inhibition in the pro-inflammatory Th1/Th17 cells and increased induction of anti-inflammatory Th2/Treg cells. In the current study, we determined whether these effects resulted from an alteration in gut microbiota and the short-chain fatty acid (SCFA) production in CD44KO mice. Fecal transfer from naïve CD44KO but not CD44WT mice, into EAE-induced CD44WT mice, led to significant amelioration of EAE. High-throughput bacterial 16S rRNA gene sequencing, followed by clustering sequences into operational taxonomic units (OTUs) and biochemical analysis, revealed that EAE-induced CD44KO mice showed significant diversity, richness, and evenness when compared to EAE-induced CD44WT mice at the phylum level, with dominant Bacteroidetes (68.5%) and low Firmicutes (26.8%). Further, data showed a significant change in the abundance of SCFAs, propionic acid and i-butyric acid in EAE-CD44KO compared to EAE-CD44WT mice. In conclusion, our results demonstrate that the attenuation of EAE seen following CD44 gene deletion in mice may result from alterations in the gut microbiota and SCFAs. Furthermore, our studies also demonstrate that the phenotype of gene knock-out animals may be shaped by gut microbiota.

Published
2017

27. Intramuscular injection of exogenous leptin induces adiposity, glucose intolerance and fatty liver by repressing the JAK2-STAT3/PI3K pathway in a rat model

Author

Wu, Lihong, primary, Chen, Guoxiong, additional, Liu, Wen, additional, Yang, Xuechao, additional, Gao, Jie, additional, Huang, Liwen, additional, Guan, Hongbing, additional, Li, Zhengmao, additional, Zheng, Zhichao, additional, Li, Meiling, additional, Gu, Weiwang, additional, and Ge, Linhu, additional

Published
2017
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28. CD44 deletion leading to attenuation of experimental autoimmune encephalomyelitis results from alterations in gut microbiome in mice

Author

Chitrala, Kumaraswamy Naidu, primary, Guan, Hongbing, additional, Singh, Narendra P., additional, Busbee, Brandon, additional, Gandy, Alexa, additional, Mehrpouya‐Bahrami, Pegah, additional, Ganewatta, Mitra S., additional, Tang, Chuanbing, additional, Chatterjee, Saurabh, additional, Nagarkatti, Prakash, additional, and Nagarkatti, Mitzi, additional

Published
2017
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30. Comparison of bacterial adhesion and biofilm formation on zirconia fabricated by two different approaches: an in vitroand in vivostudy

Author

Chen, Liyu, Yang, Shuang, Yu, Pei, Wu, Jincheng, Guan, Hongbing, and Wu, Zhe

Abstract

ABSTRACTPrepared by dry and wet approaches, respectively, the surface properties and bacterial adhesion of conventional zirconia (CZ) and self-glazed zirconia (SGZ) were investigated. Each zirconia was divided into unpolished and polished groups. Our results revealed that SGZ showed significantly lower surface roughness than CZ (P < 0.05) and lower surface-free energy (P < 0.05) in both polished and unpolished groups. The number of bacteria attached to SGZ was less than CZ in vitro(P < 0.05), but no significant difference was found between two kinds of zirconia in vivo(P > 0.05). Our findings suggest that SGZ, due to its excellent surface characteristics, is superior to CZ in bacterial adhesion and zirconia polishing serves as an indispensable procedure in clinics.

Published
2020
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32. Therapeutic effects of 3,3′-diindolylmethane on the alveolar bone in mice with experimentally induced periodontitis.

Author

Jiang, Qianzhou, Guan, Hongbing, He, Jinquan, Li, Baishun, and Pan, Jingjing

Subjects
*DIINDOLYLMETHANE, *BONE resorption, *PERIODONTITIS, *CYTOKINES, *REVERSE transcriptase polymerase chain reaction, *ANTI-inflammatory agents, *THERAPEUTICS
Abstract

In this study, we investigated effects of 3,3′-diindolylmethane (DIM) on alveolar bone resorption in mice with experimentally induced periodontitis. Experimental periodontitis was induced by placing 5.0 silk sutures around the maxillary left second molar for 1 week. Fifty-three SPF, 6-8-week-old, female C57 mice were randomly divided into four groups: (1) healthy mice (control group), (2) mice with experimentally induced periodontitis (Ped group), (3) mice with periodontitis treated with corn oil (OIL-Ped group) and (4) mice with periodontitis treated with DIM dissolved in corn oil (DIM-Ped group). Infiltration of inflammatory cells in the gingival tissue was assessed via histomorphometrical analysis, inflammatory factors were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and changes in the alveolar bone were detected by micro-computed tomography (micro-CT) after treatment for 1 and 4 weeks. After 1 week of treatment, compared with the Ped and OIL-Ped groups, the DIM-Ped group showed decreased infiltration of inflammatory cells, preservation of connective tissue in the papilla and less interproximal and interradicular bone resorption. In the DIM-Ped group, the expression of IL-1β and RANKL decreased significantly (P < 0.05). After 4 weeks of treatment, periodontal inflammation level and alveolar bone absorption were similar among groups. However, in the DIM-Ped group, the expression of IL-1β, IL-6 and RANK significantly decreased (P < 0.05). As a whole, DIM treatment for 1 week can benefit alveolar bone recovery by inhibiting the inflammation of the periodontal tissue and expression of bone resorption-related cytokines. [ABSTRACT FROM AUTHOR]

Published
2018
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