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3 results on '"Guang-wen WANG"'

1. Generation and application of two monoclonal antibodies targeting conserved linear epitopes in the NP protein of influenza A virus

Author

Yu-hui ZHAO, Xia WEN, Qi-bing LI, Li JIANG, Guang-wen WANG, Li-bin LIANG, Xiu-rong WANG, Hua-lan CHEN, and Cheng-jun LI

Subjects
influenza A virus, nucleoprotein, monoclonal antibody, application, Agriculture (General), S1-972
Abstract

Monoclonal antibodies (mAbs) are widely used in virus research and disease diagnosis. The nucleoprotein (NP) of influenza A virus (IAV) plays important roles in multiple stages of the virus life cycle. Therefore, generating conserved mAbs against NP and characterizing their properties will provide useful tools for IAV research. In this study, two mAbs against the NP protein, 10E9 and 3F3, were generated with recombinant truncated NP proteins (NP-1 and NP-2) as immunogens. The heavy-chain subclass of both 10E9 and 3F3 was determined to be IgG2α, and the light-chain type was κ. Truncation and site-specific mutation analyses showed that the epitopes of mAbs 10E9 and 3F3 were located in the N terminal 84–89 amino acids and the C terminal 320–324 amino acids of the NP protein, respectively. We found that mAbs 10E9 and 3F3 reacted well with the NP protein of H1–H15 subtypes of IAV. Both 10E9 and 3F3 can be used in immunoprecipitation assay, and 10E9 was also successfully applied in confocal microscopy. Furthermore, we found that the 10E9-recognized 84SAGKDP89 epitope and 3F3-recognized 320ENPAH324 epitope were highly conserved in NP among all avian and human IAVs. Thus, the two mAbs we developed could be used as powerful tools in the development of diagnostic methods of IAV, and also surely promote the basic research in understanding the replication mechanisms of IAV.

Published
2022
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2. Evaluation of mean diffusion and kurtosis MRI mismatch in subacute ischemic stroke: Comparison with NIHSS score

Author

Lingmei Kong, Guang-wen Wang, Renhua Wu, Zhiwei Shen, Hai-bing Rao, Guishan Zhang, Zhong-ping Zhang, Wei Chen, Wen-bin Zheng, and Yue-lin Guo

Subjects
Adult, Male, medicine.medical_specialty, Pathology, Ischemia, Infarction, Lesion Number, Severity of Illness Index, Brain Ischemia, 030218 nuclear medicine & medical imaging, Lesion, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Humans, Diagnosis, Computer-Assisted, Molecular Biology, Stroke, Aged, Aged, 80 and over, Neurologic Examination, medicine.diagnostic_test, business.industry, Cerebral infarction, General Neuroscience, Brain, Magnetic resonance imaging, Middle Aged, medicine.disease, Diffusion Magnetic Resonance Imaging, Data Interpretation, Statistical, Cardiology, Female, Neurology (clinical), medicine.symptom, Complication, business, 030217 neurology & neurosurgery, Developmental Biology
Abstract

Neurological deterioration (ND) is a devastating complication following ischemic stroke. This study aimed to identify the differences in lesion characteristics in subacute ischemic stroke patients with and without ND using diffusional kurtosis imaging (DKI), as well as to confirm the responsible lesions that may lead to ND, as assessed by the National Institutes of Health Stroke Scale (NIHSS) score. Seventy-nine patients with subacute cerebral infarction were allocated to the ND (-) and ND (+) groups according to the NIHSS score and lesion number. The mean diffusion (MD) lesions were significantly larger than the mean kurtosis (MK) deficits in the ND (+) group (P0.05); however, there was no significant difference in the ND (-) group (P0.05). The MD and MK in the lesion recovered to normal levels over time; however, the recovery trends in the ND (+) group were substantially slower than the ND (-) group. The differences between the two groups were only significant regarding the MK (p0.05). Furthermore, multiple infarction lesions exhibited good consistency in the ND (-) group, but were non-homogeneous in the ND (+) group. To the best of our knowledge, this is the first study to demonstrate that a significant MD/MK mismatch and heterogeneity of multiple ischemic lesions on MK in subacute ischemic stroke may represent a new expansion of an ischemic lesion or acute reinfarction, which is closely related to ND.

Published
2016

3. Task Scheduling in Multi-Process with Resource Constraints under MG Workflow

Author

Guang Wen Wang, Yu Ying Wang, Feng Xiong, and Yi Ping Yuan

Subjects
Rate-monotonic scheduling, Earliest deadline first scheduling, Computer science, Distributed computing, General Engineering, Scheduling (production processes), Flow shop scheduling, Dynamic priority scheduling, Round-robin scheduling, Workflow engine, Fair-share scheduling, Deadline-monotonic scheduling, Stride scheduling, Scheduling (computing), Fixed-priority pre-emptive scheduling, Workflow, Genetic algorithm scheduling, Two-level scheduling, Critical path method, Workflow management system
Abstract

In manufacturing Grid workflow, multiple tasks share a common and limited resource pool. In order to solve task scheduling in multi-process with resource constraints under MG workflow, the Task-Resource Constrained model is set up to descript the assignment relation between task and resource. The framework of the task scheduling and the scheduling policies are also presented that can readjust the tasks according to the priority rules and the time parameters in the process. Then we present a heuristic scheduling algorithm that includes multiple policies. The heuristic scheduling algorithm will update the critical path of DAG (Direct Acyclic Graph) and the beginning time of post-tasks. MG Workflow engine can dynamically schedule the resources according the task requirement. An example is given to illustrate the method at last.

Published
2008

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