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Cellular analysis of body fluids (BFs) can assist clinicians for the diagnosis of many medical conditions. The aim of this work is the evaluation of the analytical performance of the UF-5000 body fluid mode (UF-BF) analyzer compared to the gold standard method (optical microscopy, OM) and to XN-1000 (XN-BF), another analyzer produced by the same manufacturer (Sysmex) and with a similar technology for BF analysis. One hundred BF samples collected in K3EDTA tubes were analyzed by UF-BF, XN-BF and OM. The agreement was evaluated using Passing and Bablok regression and Bland–Altman plot analysis. The receiver operating characteristic (ROC) curves were selected for evaluating the diagnostic agreement between OM classification and UF-BF parameters. Comparison between UF-BF and OM, in all BF types, showed Passing and Bablok's slope comprised between 0.99 (polymorphonuclear cells count, PMN-BF) and 1.39 (mononuclear cells count, MN-BF), the intercepts ranged between 26.47 (PMN-BF parameter) and 226.80 (white blood cell count). Bland–Altman bias was comprised between 7.3% (total cell count, TC-BF) and 52.9% (MN-BF). Comparison between UF-BF and XN-BF in all BF showed slopes ranged between 1.07 (TC-BF and PMN-BF) and 1.16 (MN-BF), intercepts ranged between 8.30 (PMN) and 64.78 (WBC-BF). Bland–Altman bias ranged between 5.8 (TC-BF) and 21.1% (MN-BF). The ROC curve analysis showed an area under the curve ranged between 0.9664 and 1.000. UF-BF shows very good performance for the differential counts of cells in ascitic, pleural and synovial fluids and therefore it is useful to screen and count cells in this type of BF. [ABSTRACT FROM AUTHOR]

My commentary on the positional paper aims to contribute to the goal of extending psychoanalysis to an analysis and intervention in the social systems. It is not a question of "applying" psychoanalysis outside the dual clinical context but of identifying the elements that allow it to be used correctly in non-classical clinical contexts. In this perspective, my article revisits Massenpsychologie und Ich-Analyze, in which Freud, while demonstrating the potential of psychoanalysis for attaining an understanding of social processes, moves within implicit presuppositions that can result in epistemic and technical obstacles to the very extension of psychoanalysis. In this sense, the article discusses three issues. A first issue is the legitimacy of defining the social in its various components (individual, groups, organizational) as a set of levels made up of unconscious and conscious variables, each endowed with emerging specificities (autonomy) that cannot be reduced to simpler underlying levels. A second issue concerns the fact that analyzing unconscious processes present in social systems, such as identifications, defense mechanisms, desires and fantasies, cannot ignore the complex relationship with organizational aspects (objectives, roles, functions, technologies), i.e., with conscious and socially shared projects. The third issue is the need to historicize, i.e., to interpret the signs of the times, the specificity of the historical moment that determines certain forms of social and cultural life. [ABSTRACT FROM AUTHOR]

Recent data indicate that the Simian virus 40 (SV40) infection appears to be transmitted in humans independently from early SV40‐contaminated antipolio vaccines. Serum antibodies against SV40 large T antigen (Tag) were analyzed in children/adolescents and young adults. To investigate antibodies reacting to SV40 Tag antigens, serum samples (n = 812) from children and young adults were analyzed by indirect ELISAs using specific SV40 Tag mimotopes. Mimotopes were synthetic peptides corresponding to SV40 Tag epitopes. In sera (n = 412) from healthy children up to 17 years old, IgG antibodies against SV40 Tag mimotopes reached an overall prevalence of 15%. IgM antibodies against SV40 Tag were detected in sera of children 6–8 months old confirming and extending the knowledge that SV40 seroconversion occurs early in life. In children/adolescents affected by different diseases (n = 180) SV40 Tag had a prevalence of 18%, being the difference no significant compared to healthy subjects (n = 220; 16%) of the same age. Our immunological data indicate that SV40 circulates in children and young adults, both in healthy conditions and affected by distinct diseases. The IgM detection in sera from healthy children suggests that the SV40 infection/seroconversion occurs early in life (>6 months). Our immunological data support the hypothesis that SV40, or a closely related still unknown polyomavirus, infects humans. The SV40 seroprevalence is lower than common polyomaviruses, such as BKPyV and JCPyV, and other new human polyomaviruses. In addition, our immunological surveillance indicates a lack of association between different diseases, considered herein, and SV40. Antibodies reacting to SV40 Tag antigens were investigated in serum samples (n = 812) from children and young adults by indirect ELISAs using specific SV40 Tag mimotopes. IgG had an overall prevalence of 15% and 18% in healthy subjects and young patients, respectively. IgM antibodies against SV40 Tag were detected in sera of children 6–8 months old confirming and extending the knowledge that SV40 seroconversion occurs early in life. [ABSTRACT FROM AUTHOR]