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Additional file 2: Table S2. Sociodemographic and clinical characteristics in sub-group analyses

Additional file 1: Table S1. Hazard ratio (HR) and corresponding 95% confidence interval (CI) for progression-free survival and overall survival in 163 patients with stage III–IV oropharyngeal squamous cell carcinoma according to combination of HPV status and LINE-1 methylation

A device and method to compensate for distortions of amplitude that afflicts systems for communicating through capacitive coupling.

A method for verifying alignment between two integrated circuits connected to form a 3D stack. The method is based on a reference communication channel and several adjacent channels: the difference in the received signals allows to detect misalignments in x,y and z directions

We present the development and application of a microfluidic device that can efficiently and reproducibly isolate a programmable number of cells into microwells. The chip consists of a 3x4 array of 100 µm microwells drilled through a flex-PCB substrate along microchannels. Negative DEP focusing of particles is used to axially centre particles within the channel and also to shield the microwell entrance for flowing particles. An optical detection routine analyses and selects the cells to be delivered with single cell resolution. Cell viability results based on a calcein release assay performed on single cells are within the range of physiological loss of fluorescence intensity.

We present a novel method for the isolation, functional analysis and sorting of single cells or small cellular aggregates. In- verted open microwells featuring a microchannel on top, an open air-fluid interface on bottom side and embedded electrodes were implemented on flexible-PCB technology. Microwell arrays in pitch with standard microtiter plates were implemented. K562 cells were delivered to the microwells and trapped at the air-flow interface. Cell delivery and aggregate formation were controlled by dielectrophoresis. Continuous fluid replacement around the cells during the experiment was demonstrated. After analysis, live cells were easily recovered to standard plates where cell growth was demonstrated.

We present here an innovative architecture based on negative-dielectrophoresis (nDEP) to perform the controlled isolation and patterning of a programmable number of cells inside microwells. The high efficiency solution we propose features electrodes placed on the floor and sidewalls of the microfluidic channels where microwells are realized and allows for the successful isolation, patterning and live-cell imaging of both single cells and cell clusters, with single cell resolution. Cell viability was assessed after delivery performing a calcein release assay in the microwells.

In this work we present a method for delivering small fluid volumes possibly containing cells and particles over a flexible large area substrate. Particles are deposited by a dispensing device that performs serial delivery of liquid volumes in the range of 200 pL. Due to the required precision, the alignment and the calibration of the spotting system are crucial for the proper performance of the delivery process. The system provides automated motion of mechanical parts with micrometric precision. To address the problem of the device deformation caused by the flexibility of the substrate, the 3D coordinates of each site need to be known, but the serial acquisition of all the coordinates would be highly time consuming for high-throughput arrays and large devices. We solved this problem by sampling the coordinates of a small subset and interpolating the positions of the remaining sites by means of a custom algorithm. System calibration for%2

We present here a lab-on-a-chip to perform cell centrifugation and re-suspension at single-cell level. Microchannels are used to isolate cells inside microwells where fluid replacement can be obtained thanks to microfluidic mixing. Electrodes integrated into each microwell were used to detect conductivity variation of the infused fluids allowing also for a monitored and automated execution of biological protocols which require supernatant substitution. A protocol involving a precise replacement of the supernatants as required by a cell viability assay was implemented demonstrating single-cell microcentrifugation and re-suspension capabilities as confirmed by calcein uptake shown by fluorescence microscopy.

This paper describes a System on Chip implementation of a reconfigurable digital signal processor. The device is suitable for execution of a wide range of applications exploiting a balanced mix of heterogeneous reconfigurable fabrics merged together by a flexible and efficient communication infrastructure based on a 64-bit Network On Chip. The SoC combines a fine grain embedded FPGA, a mid grain configurable processor and a coarse grain reconfigurable array. An ARM processor featuring a resident operating system is the SoC supervisor, managing communication, synchronization and reconfiguration mechanisms. This computational model enables the programmer to manage the high level synchronization and global data of complex signal processing applications through the ARM processor, while allocating most critical computational kernels to the most suitable reconfigurable engines. The SoC has been fabricated in 90-nm technology, the die area being 110mm2

In this article we present an impedance-based measurement technique for single-cell detection within open microwells. It is used for continuous monitoring of cells delivered in open-microwell arrays which enable simplified cell recovery procedures.