1. Effects of Lactobacillus plant arum on genes expression pattern in mice jejunal Peyer's patches
- Author
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Yonghui Shi, Jin Sun, Jianing Li, Ziwei Xu, Gui-fang Chang, and Guowei Le
- Subjects
Male ,Cellular differentiation ,Gene Expression Profiling ,Probiotics ,Immunology ,Gene Expression ,Biology ,Microbiology ,Cell biology ,Jejunum ,Gene expression profiling ,Mice, Inbred C57BL ,Lactobacillus ,Mice ,Peyer's Patches ,medicine.anatomical_structure ,Immune system ,Transcytosis ,Gene expression ,medicine ,Animals ,Signal transduction ,Intestinal Mucosa ,Microfold cell - Abstract
Jejunal Peyer's patches contain specialized epithelial M cells that take up ingested microorganisms from the lumen of the gut by transcytosis. Using DNA-micro array, we analyzed the gene expression patterns of jejunal Peyer's patches in order to gain insight into the molecular mechanism by which Lp6 interacted with the host organism in a gnotobiotic environment v. in the gut normal microflora. The micro array data revealed that, among approximately 14,000 genes, 420 were expressed in Lp6 administration group at twofold or higher levels compared to the control group. These included genes involved in immune response, and cell differentiation, cell-cell signaling, cell adhesion, signal transcription, and transduction. Real-time PCR confirmed the reliability of the analysis. These data indicated that administration of Lactobacillus Lp6 was associated with a complex genetic response in the jejunal Peyer's patches.
- Published
- 2008