11 results on '"Guillaume Lardier"'
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2. Supplementary Figure 4 from Characterization of a Large Panel of Patient-Derived Tumor Xenografts Representing the Clinical Heterogeneity of Human Colorectal Cancer
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Cyril Berthet, Olivier Duchamp, Sergio Roman-Roman, Patricia Vrignaud, Grégoire Prévost, Brigitte Demers, Loreley Calvet, Guillaume Lardier, Manoel Nunes, Hany Soliman, Hugues De Thé, Alain Pierré, Alain Bruno, Philippe Dessen, Bastien Job, Virginie Dangles-Marie, Patrick Gonin, Emmanuel Pham, Loïc Morgand, Denis Lantuas, Louis-Bastien Weiswald, Peggy Dartigues, Fariba Nemati, Ludovic Bigot, Sophie Landron, Pascale Mariani, Diane Goéré, Marc Pocard, Ludovic Lacroix, Ana Merino-Trigo, and Sylvia Julien
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PDF file, 923K, Comparison of gene expression between primary tumor sample, early and late xenograft sample.
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- 2023
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3. Supplementary Tables 1 - 4 from Characterization of a Large Panel of Patient-Derived Tumor Xenografts Representing the Clinical Heterogeneity of Human Colorectal Cancer
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Cyril Berthet, Olivier Duchamp, Sergio Roman-Roman, Patricia Vrignaud, Grégoire Prévost, Brigitte Demers, Loreley Calvet, Guillaume Lardier, Manoel Nunes, Hany Soliman, Hugues De Thé, Alain Pierré, Alain Bruno, Philippe Dessen, Bastien Job, Virginie Dangles-Marie, Patrick Gonin, Emmanuel Pham, Loïc Morgand, Denis Lantuas, Louis-Bastien Weiswald, Peggy Dartigues, Fariba Nemati, Ludovic Bigot, Sophie Landron, Pascale Mariani, Diane Goéré, Marc Pocard, Ludovic Lacroix, Ana Merino-Trigo, and Sylvia Julien
- Abstract
PDF file, 129K, Table S1: Description of sequenced exons. Table S2: tumor growth observed in the first engraftment. Table S3: Tumor samples for which development of metastasis has been observed after an engraftment into the cecum. Table S4: Ontology analysis of gene differentially expressed between patient tumors and xenografts.
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- 2023
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4. CCR Translation for This Article from Characterization of a Large Panel of Patient-Derived Tumor Xenografts Representing the Clinical Heterogeneity of Human Colorectal Cancer
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Cyril Berthet, Olivier Duchamp, Sergio Roman-Roman, Patricia Vrignaud, Grégoire Prévost, Brigitte Demers, Loreley Calvet, Guillaume Lardier, Manoel Nunes, Hany Soliman, Hugues De Thé, Alain Pierré, Alain Bruno, Philippe Dessen, Bastien Job, Virginie Dangles-Marie, Patrick Gonin, Emmanuel Pham, Loïc Morgand, Denis Lantuas, Louis-Bastien Weiswald, Peggy Dartigues, Fariba Nemati, Ludovic Bigot, Sophie Landron, Pascale Mariani, Diane Goéré, Marc Pocard, Ludovic Lacroix, Ana Merino-Trigo, and Sylvia Julien
- Abstract
CCR Translation for This Article from Characterization of a Large Panel of Patient-Derived Tumor Xenografts Representing the Clinical Heterogeneity of Human Colorectal Cancer
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- 2023
- Full Text
- View/download PDF
5. Data from Characterization of a Large Panel of Patient-Derived Tumor Xenografts Representing the Clinical Heterogeneity of Human Colorectal Cancer
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Cyril Berthet, Olivier Duchamp, Sergio Roman-Roman, Patricia Vrignaud, Grégoire Prévost, Brigitte Demers, Loreley Calvet, Guillaume Lardier, Manoel Nunes, Hany Soliman, Hugues De Thé, Alain Pierré, Alain Bruno, Philippe Dessen, Bastien Job, Virginie Dangles-Marie, Patrick Gonin, Emmanuel Pham, Loïc Morgand, Denis Lantuas, Louis-Bastien Weiswald, Peggy Dartigues, Fariba Nemati, Ludovic Bigot, Sophie Landron, Pascale Mariani, Diane Goéré, Marc Pocard, Ludovic Lacroix, Ana Merino-Trigo, and Sylvia Julien
- Abstract
Purpose: Patient-derived xenograft models are considered to represent the heterogeneity of human cancers and advanced preclinical models. Our consortium joins efforts to extensively develop and characterize a new collection of patient-derived colorectal cancer (CRC) models.Experimental Design: From the 85 unsupervised surgical colorectal samples collection, 54 tumors were successfully xenografted in immunodeficient mice and rats, representing 35 primary tumors, 5 peritoneal carcinoses and 14 metastases. Histologic and molecular characterization of patient tumors, first and late passages on mice includes the sequence of key genes involved in CRC (i.e., APC, KRAS, TP53), aCGH, and transcriptomic analysis.Results: This comprehensive characterization shows that our collection recapitulates the clinical situation about the histopathology and molecular diversity of CRC. Moreover, patient tumors and corresponding models are clustering together allowing comparison studies between clinical and preclinical data. Hence, we conducted pharmacologic monotherapy studies with standard of care for CRC (5-fluorouracil, oxaliplatin, irinotecan, and cetuximab). Through this extensive in vivo analysis, we have shown the loss of human stroma cells after engraftment, observed a metastatic phenotype in some models, and finally compared the molecular profile with the drug sensitivity of each tumor model. Through an experimental cetuximab phase II trial, we confirmed the key role of KRAS mutation in cetuximab resistance.Conclusions: This new collection could bring benefit to evaluate novel targeted therapeutic strategies and to better understand the basis for sensitivity or resistance of tumors from individual patients. Clin Cancer Res; 18(19); 5314–28. ©2012 AACR.
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- 2023
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6. Supplementary Figure 2 from Characterization of a Large Panel of Patient-Derived Tumor Xenografts Representing the Clinical Heterogeneity of Human Colorectal Cancer
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Cyril Berthet, Olivier Duchamp, Sergio Roman-Roman, Patricia Vrignaud, Grégoire Prévost, Brigitte Demers, Loreley Calvet, Guillaume Lardier, Manoel Nunes, Hany Soliman, Hugues De Thé, Alain Pierré, Alain Bruno, Philippe Dessen, Bastien Job, Virginie Dangles-Marie, Patrick Gonin, Emmanuel Pham, Loïc Morgand, Denis Lantuas, Louis-Bastien Weiswald, Peggy Dartigues, Fariba Nemati, Ludovic Bigot, Sophie Landron, Pascale Mariani, Diane Goéré, Marc Pocard, Ludovic Lacroix, Ana Merino-Trigo, and Sylvia Julien
- Abstract
PDF file, 241K, Frequency of abnormalities detected by aCGH among all samples tested across the genome.
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- 2023
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7. Supplementary Figure 3 from Characterization of a Large Panel of Patient-Derived Tumor Xenografts Representing the Clinical Heterogeneity of Human Colorectal Cancer
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Cyril Berthet, Olivier Duchamp, Sergio Roman-Roman, Patricia Vrignaud, Grégoire Prévost, Brigitte Demers, Loreley Calvet, Guillaume Lardier, Manoel Nunes, Hany Soliman, Hugues De Thé, Alain Pierré, Alain Bruno, Philippe Dessen, Bastien Job, Virginie Dangles-Marie, Patrick Gonin, Emmanuel Pham, Loïc Morgand, Denis Lantuas, Louis-Bastien Weiswald, Peggy Dartigues, Fariba Nemati, Ludovic Bigot, Sophie Landron, Pascale Mariani, Diane Goéré, Marc Pocard, Ludovic Lacroix, Ana Merino-Trigo, and Sylvia Julien
- Abstract
PDF file, 67K, Hierarchical clustering of 82 tumors based on Chromosomal abnormalities analyzed by 244k aCGH.
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- 2023
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8. Supplementary Figure Legend from Characterization of a Large Panel of Patient-Derived Tumor Xenografts Representing the Clinical Heterogeneity of Human Colorectal Cancer
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Cyril Berthet, Olivier Duchamp, Sergio Roman-Roman, Patricia Vrignaud, Grégoire Prévost, Brigitte Demers, Loreley Calvet, Guillaume Lardier, Manoel Nunes, Hany Soliman, Hugues De Thé, Alain Pierré, Alain Bruno, Philippe Dessen, Bastien Job, Virginie Dangles-Marie, Patrick Gonin, Emmanuel Pham, Loïc Morgand, Denis Lantuas, Louis-Bastien Weiswald, Peggy Dartigues, Fariba Nemati, Ludovic Bigot, Sophie Landron, Pascale Mariani, Diane Goéré, Marc Pocard, Ludovic Lacroix, Ana Merino-Trigo, and Sylvia Julien
- Abstract
PDF file, 72K.
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- 2023
- Full Text
- View/download PDF
9. Characterization of a large panel of patient-derived tumor xenografts representing the clinical heterogeneity of human colorectal cancer
- Author
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Sylvia Julien, Ana Merino-Trigo, Ludovic Lacroix, Marc Pocard, Diane Goéré, Pascale Mariani, Sophie Landron, Ludovic Bigot, Fariba Nemati, Peggy Dartigues, Louis-Bastien Weiswald, Denis Lantuas, Loïc Morgand, Emmanuel Pham, Patrick Gonin, Virginie Dangles-Marie, Bastien Job, Philippe Dessen, Alain Bruno, Alain Pierré, Hugues De Thé, Hany Soliman, Manoel Nunes, Guillaume Lardier, Loreley Calvet, Brigitte Demers, Grégoire Prévost, Patricia Vrignaud, Sergio Roman-Roman, Olivier Duchamp, and Cyril Berthet
- Subjects
Oncology ,Male ,Cancer Research ,medicine.medical_specialty ,Organoplatinum Compounds ,Colorectal cancer ,Cetuximab ,Bioinformatics ,medicine.disease_cause ,Antibodies, Monoclonal, Humanized ,Irinotecan ,Article ,Mice ,Internal medicine ,medicine ,Animals ,Humans ,neoplasms ,Aged ,Neoplasm Staging ,Aged, 80 and over ,business.industry ,Microsatellite instability ,Cancer ,Antibodies, Monoclonal ,Middle Aged ,medicine.disease ,Xenograft Model Antitumor Assays ,digestive system diseases ,Oxaliplatin ,Rats ,Gene Expression Regulation, Neoplastic ,Cell Transformation, Neoplastic ,Drug Resistance, Neoplasm ,Monoclonal ,Camptothecin ,Female ,Microsatellite Instability ,KRAS ,Fluorouracil ,business ,Colorectal Neoplasms ,medicine.drug - Abstract
Purpose: Patient-derived xenograft models are considered to represent the heterogeneity of human cancers and advanced preclinical models. Our consortium joins efforts to extensively develop and characterize a new collection of patient-derived colorectal cancer (CRC) models. Experimental Design: From the 85 unsupervised surgical colorectal samples collection, 54 tumors were successfully xenografted in immunodeficient mice and rats, representing 35 primary tumors, 5 peritoneal carcinoses and 14 metastases. Histologic and molecular characterization of patient tumors, first and late passages on mice includes the sequence of key genes involved in CRC (i.e., APC, KRAS, TP53), aCGH, and transcriptomic analysis. Results: This comprehensive characterization shows that our collection recapitulates the clinical situation about the histopathology and molecular diversity of CRC. Moreover, patient tumors and corresponding models are clustering together allowing comparison studies between clinical and preclinical data. Hence, we conducted pharmacologic monotherapy studies with standard of care for CRC (5-fluorouracil, oxaliplatin, irinotecan, and cetuximab). Through this extensive in vivo analysis, we have shown the loss of human stroma cells after engraftment, observed a metastatic phenotype in some models, and finally compared the molecular profile with the drug sensitivity of each tumor model. Through an experimental cetuximab phase II trial, we confirmed the key role of KRAS mutation in cetuximab resistance. Conclusions: This new collection could bring benefit to evaluate novel targeted therapeutic strategies and to better understand the basis for sensitivity or resistance of tumors from individual patients. Clin Cancer Res; 18(19); 5314–28. ©2012 AACR.
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- 2012
10. Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype
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Nicole Stange-Thomann, Michael Katinka, Michael Levy, Vincent Laudet, Olivier Jaillon, Cécile Fischer, Véronique de Berardinis, Zineb Skalli, Jill P. Mesirov, David M. Jaffe, Alain Bernot, Genís Parra, Simone Duprat, Claude Scarpelli, Daniel Kahn, Chad Nusbaum, Sophie Nicaud, Laurence Bouneau, Francis Quetier, Hugues Roest Crollius, Corinne Cruaud, Bruce W. Birren, Jérôme Gouzy, Kevin McKernan, Eric S. Lander, Nathalie Boudet, Vanina Castelli, Marc Robinson-Rechavi, Jean-Pierre Coutanceau, Béatrice Segurens, Michael C. Zody, Corinne Dasilva, Evan Mauceli, Paul A. McEwan, Manolis Kellis, Carole Dossat, Benoit Vacherie, Marcel Salanoubat, Roderic Guigó, Sheila Fisher, Christian Biémont, Stephanie Bosak, Jean Weissenbach, Véronique Anthouard, Jean-Nicolas Volff, William Saurin, Vincent Schächter, Julie Poulain, Catherine Ozouf-Costaz, Jean-Louis Petit, Laurence Cattolico, Guillaume Lardier, Charles Chapple, Sergi Castellano, Georges Lutfalla, Frédéric Brunet, Kerstin Lindblad-Toh, Philippe Brottier, Claire Jubin, Patrick Wincker, Jean-Marc Aury, Eléments transposables, évolution, populations, Département génétique, interactions et évolution des génomes [LBBE] (GINSENG), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Bioinformatique, phylogénie et génomique évolutive (BPGE), Département PEGASE [LBBE] (PEGASE), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Unité mixte de recherche biologie moléculaire de la cellule, École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Broad Institute of MIT and Harvard (BROAD INSTITUTE), Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT)-Massachusetts General Hospital [Boston], Department of Atmospheric and Environmental Chemistry [Bothell], University of Washington-Bothell, Bayer Cropscience, Centre d'étude spatiale des rayonnements (CESR), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS)
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Genome evolution ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,Proteome ,[SDV]Life Sciences [q-bio] ,Molecular Sequence Data ,2R hypothesis ,Tetraodon nigroviridis ,Fish Proteins ,Synteny ,Genome ,Chromosomes ,Evolution, Molecular ,Gene Duplication ,biology.animal ,Animals ,Chromosomes, Human ,Humans ,Urochordata ,Tetraodon ,Conserved Sequence ,Mammals ,Genetics ,Base Composition ,Multidisciplinary ,Models, Genetic ,biology ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,Fishes ,Vertebrate ,Sequence Analysis, DNA ,Physical Chromosome Mapping ,biology.organism_classification ,Genes ,Karyotyping ,Vertebrates ,Human genome - Abstract
International audience; Tetraodon nigroviridis is a freshwater puffer fish with the smallest known vertebrate genome. Here, we report a draft genome sequence with long-range linkage and substantial anchoring to the 21 Tetraodon chromosomes. Genome analysis provides a greatly improved fish gene catalogue, including identifying key genes previously thought to be absent in fish. Comparison with other vertebrates and a urochordate indicates that fish proteins have diverged markedly faster than their mammalian homologues. Comparison with the human genome suggests ∼900 previously unannotated human genes. Analysis of the Tetraodon and human genomes shows that whole-genome duplication occurred in the teleost fish lineage, subsequent to its divergence from mammals. The analysis also makes it possible to infer the basic structure of the ancestral bony vertebrate genome, which was composed of 12 chromosomes, and to reconstruct much of the evolutionary history of ancient and recent chromosome rearrangements leading to the modern human karyotype.
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- 2004
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11. Analysis of 148 kb of genomic DNA of Tetraodon nigroviridis covering an amylase gene family
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Laurence Bouneau, Jean Weissenbach, Guillaume Lardier, Cécile Fischer, Alain Bernot, and Muriel Ronsin
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DNA, Complementary ,Molecular Sequence Data ,Biology ,Tetraodon nigroviridis ,Biochemistry ,Genome ,Synteny ,Endocrinology ,Intergenic region ,Genetics ,Humans ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,Gene ,Sequence Homology, Amino Acid ,cDNA library ,Tetraodontiformes ,Chromosome Mapping ,DNA ,Sequence Analysis, DNA ,biology.organism_classification ,genomic DNA ,Chromosomes, Human, Pair 1 ,Amylases ,Human genome ,Sequence Alignment ,Software - Abstract
We have sequenced and analysed a 148 kb genomic region of Tetraodon nigroviridis, a teleost fish with a compact genome. Several genes were identified by comparison with genomic or transcript sequences of other species, informatic prediction and screening of a cDNA library. As expected for a compact genome, sizes of the identified genes and introns are very small, and intergenic distances are short. Among identified genes, three code for amylases. As in mammals, these genes are linked, but they are found in a small region of less than 11 kb. These results represent the first description of a genomic sequence larger than 100 kb in this species. Synteny with the human genome is restricted to three regions corresponding to human 1p32.3, 1p13.3 and 1p21.1.
- Published
- 2003
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