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Histochemical staining of histological sections of Varroa destructor (Anderson and Trueman, 2000) mites reveal the internal body plan and are used to contrast the internal organs associated with feeding and reproduction of starved versus recently fed female mites. The gnathosoma is comprised of a powerful sucking pharynx, which employs 11 alternating dilator and constrictor muscles, the chelicerae, the salivary ducts, and the salivarium. Coronally, the esophagus is visible through the synganglion between its supraesophageal and subesophageal regions and connects posteriorly to the midgut. The midgut is devoid of food particles in starved mites, but in fed mites, the midgut epithelial cells are filled with innumerable globular spheroids replete with lipoproteins, including polyunsaturated lipids, whereas the lumen is filled with saturated lipids or other unidentified nutrients. In the opisthosomal body region of the fed female, the bilobed lyrate organ lies adjacent to the midgut on one side and the ovary on the opposite side, with very close cell to cell linkages that appear to form a syncytium. The fed female ovary contains an enormously enlarged ovum, and numerous elongated nurse cells extending from the lyrate organ. Dyes staining selectively for lipoproteins suggests rapid incorporation of neutral and polyunsaturated lipids and lipoproteins. Also evident near the ovary in fed females is the spermatheca filled with elongated, fully capacitated spermatozoa. The histological and histochemical findings reported in this study provide a fresh insight into the body structure, nutrition, and reproductive activity of the female of this harmful honey bee parasite and disease vector. [ABSTRACT FROM AUTHOR]

Single-cell sequencing studies have characterized the transcriptomic signature of cell types within the kidney. However, the spatial distribution of acute kidney injury (AKI) is regional and affects cells heterogeneously. We first optimized coordination of spatial transcriptomics and single-nuclear sequencing data sets, mapping 30 dominant cell types to a human nephrectomy. The predicted cell-type spots corresponded with the underlying histopathology. To study the implications of AKI on transcript expression, we then characterized the spatial transcriptomic signature of 2 murine AKI models: ischemia/reperfusion injury (IRI) and cecal ligation puncture (CLP). Localized regions of reduced overall expression were associated with injury pathways. Using single-cell sequencing, we deconvoluted the signature of each spatial transcriptomic spot, identifying patterns of colocalization between immune and epithelial cells. Neutrophils infiltrated the renal medulla in the ischemia model. Atf3 was identified as a chemotactic factor in S3 proximal tubules. In the CLP model, infiltrating macrophages dominated the outer cortical signature, and Mdk was identified as a corresponding chemotactic factor. The regional distribution of these immune cells was validated with multiplexed CO-Detection by indEXing (CODEX) immunofluorescence. Spatial transcriptomic sequencing complemented single-cell sequencing by uncovering mechanisms driving immune cell infiltration and detection of relevant cell subpopulations.