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A new type of hybrid material (NCMTs@MoO2/FeNi3) with a multi-layer heterostructure was designed and fabricated via a one-step pyrolysis process using FeOOH/NiMoO4@PDA as the precursor. FeOOH/NiMoO4@PDA was prepared by the solvothermal method, followed by the nickel-ion etching method coupled with the polymerization of dopamine (DA). The as-obtained material was made of nitrogen-doped carbon nanotubes embedded with FeNi3 and MoO2 nanoparticles (NPs). Notably, the FeNi3 NPs exhibited significantly improved performance in the reduction of 4-nitrophenol (4-NP) and adsorption of histidine-rich protein as well as provided appropriate magnetism resources. The MoO2 NPs imparted a metallic nature with excellent conductivity, and the N-doped mesoporous carbon microtubes also improved conductivity and facilitated mass transfer, thus leading to enhanced performance in catalysis. Benefiting from the 1D hierarchical porous structure and compositional features, the NCMTs@MoO2/FeNi3 composites exhibited excellent performance in 4-NP reduction and protein adsorption via specific metal affinity between the polyhistidine groups of proteins and the FeNi3 NPs. The result presented here indicates that the strategy of combining tailored components, heterostructuring, and carbon integration is a promising way to obtain high-performance composites for other energy-related applications. [ABSTRACT FROM AUTHOR]

The emerging new variants of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) needs booster vaccination. We evaluated the long‐term safety and immunogenicity of heterologous boosting with a SARS‐CoV‐2 messenger RNA vaccine SYS6006. A total of 1000 participants aged 18 years or more who had received two (Group A) or three (Group B) doses of SARS‐CoV‐2 inactivated vaccine were enrolled and vaccinated with one dose of SYS6006 which was designed based on the prototype spike protein and introduced mutation sites. Adverse events (AEs) through 30 days and serious AEs during the study were collected. Live‐virus and pseudovirus neutralizing antibody (Nab), binding antibody (immunoglobulin G [IgG]) and cellular immunity were tested through 180 days. Solicited all, injection‐site and systemic AEs were reported by 618 (61.8%), 498 (49.8%), and 386 (38.6%) participants, respectively. Most AEs were grade 1. The two groups had similar safety profile. No vaccination‐related SAEs were reported. Robust wild‐type (WT) live‐virus Nab response was elicited with peak geometric mean titers (GMTs) of 3769.5 (Group A) and 5994.7 (Group B) on day 14, corresponding to 1602.5‐ and 290.8‐fold increase versus baseline, respectively. The BA.5 live‐virus Nab GMTs were 87.7 (Group A) and 93.2 (Group B) on day 14. All participants seroconverted for WT live‐virus Nab. Robust pseudovirus Nab and IgG responses to wild type and BA.5 were also elicited. ELISpot assay showed robust cellular immune response, which was not obviously affected by virus variation. In conclusion, SYS6006 heterologous boosting demonstrated long‐term good safety and immunogenicity in participants who had received two or three doses of SARS‐CoV‐2 inactivated vaccine. [ABSTRACT FROM AUTHOR]

In the realm of bridge structural engineering, it is customary to meticulously contemplate the material's strength and rigidity attributes during the dimensioning phase. In recent years, there has been a burgeoning interest in employing Ultrahigh-Performance Concrete (abbreviated as UHPC) for the construction of bridge decks and wet joints. However, the large self-shrinkage of UHPC can easily lead to shrinkage cracking and affect its service life. This study delves into the utilization of a blend of basalt coarse aggregate and high-modulus aggregate (HMA) in the formulation of Ultrahigh-Performance Concrete (UHPC) with the objectives of achieving exceptional strength (>180 MPa), superior modulus of elasticity (>56 GPa), and synergistic effect of using prewetted internal curing aggregate (ICA), metallurgical ore sand (MOS), and calcium–magnesium composite-based expansion agent (EA) to reduce the shrinkage of UHPC. Furthermore, the mechanical properties, shrinkage, hydration process, and microstructure of UHPC prepared with EA and ICA were studied. The results show that UHPC prepared with both 3% EA and 20% ICA had the optimal volume stability (the shrinkage was only 273 με at 180 d). In contrast, the 180 d shrinkage of UHPC with 3% EA and 20% ICA separately was 287 με and 373 με, respectively. In addition, the incorporation of EA and ICA can effectively improve the flexural strength of UHPC, although it affects the compressive strength and modulus of elasticity of UHPC (small decrease). [ABSTRACT FROM AUTHOR]

Utilizing the inherent high nitrogen content in natural microalgae to produce value‐added nitrogen‐containing compounds such as fatty amides and fatty nitriles is a promising method. Herein, a method for producing value‐added fatty amides and nitriles by liquefaction of natural microalgae from water blooms in n‐heptane was developed. The effects of temperature, metal oxide catalyst (ZrO2, Al2O3, TiO2, ZnO, MgO, CaO), catalyst amount, and reaction time on the preparation of value‐added nitrogen‐containing compounds were studied. Under the optimized conditions (0.3 g ZrO2, 300 °C, 6 h), the total yield of fatty amides was 6.9 wt %, and the yield of fatty nitriles was 1.9 wt %. Compared with the results obtained in the absence of ZrO2, after adding ZrO2 the total yield of fatty acids was reduced by 4.7 wt % (18.5 to 13.8 wt %), while the total yield of fatty amides only increased by 0.9 wt % (6.0 to 6.9 wt %) and fatty nitriles was increased by 1.5 wt % (0.4 to 1.9 wt %). Exploring the role of ZrO2 by using model compounds (i. e. palmitic acid and palmitamide) revealed that ZrO2 could promote the dehydration of fatty amides to form fatty nitriles, but had limited effect on the reaction of fatty acid and NH3. [ABSTRACT FROM AUTHOR]

A new type of hybrid material (NCMTs@MoO 2 /FeNi 3 ) with a multi-layer heterostructure was designed and fabricated via a one-step pyrolysis process using FeOOH/NiMoO 4 @PDA as the precursor. FeOOH/NiMoO 4 @PDA was prepared by the solvothermal method, followed by the nickel-ion etching method coupled with the polymerization of dopamine (DA). The as-obtained material was made of nitrogen-doped carbon nanotubes embedded with FeNi 3 and MoO 2 nanoparticles (NPs). Notably, the FeNi 3 NPs exhibited significantly improved performance in the reduction of 4-nitrophenol (4-NP) and adsorption of histidine-rich protein as well as provided appropriate magnetism resources. The MoO 2 NPs imparted a metallic nature with excellent conductivity, and the N-doped mesoporous carbon microtubes also improved conductivity and facilitated mass transfer, thus leading to enhanced performance in catalysis. Benefiting from the 1D hierarchical porous structure and compositional features, the NCMTs@MoO 2 /FeNi 3 composites exhibited excellent performance in 4-NP reduction and protein adsorption via specific metal affinity between the polyhistidine groups of proteins and the FeNi 3 NPs. The result presented here indicates that the strategy of combining tailored components, heterostructuring, and carbon integration is a promising way to obtain high-performance composites for other energy-related applications.

Objective: Based on the analysis of omics data of Volvariella volvacea, a gene encoding glutathione S- transferase (GSTs) named vv-gtol was obtained. To reveal the role of GSTs in the growth and development in edible fungi, the structure, the sequence characters and the expression profile of a GST gene vv-gto1 of Volvariella volvacea were analyzed., Methods: ZOOM software was used to map sequencing read (reads) from genome and transcriptome against the splicing sequence of genome, to confirm the complete length and the accuracy of the gene sequence, and to visualize gene structure. The MEGA 5.1 was used to do the multiple sequence alignment and phylogenetic tree analysis. Real time fluorescent quantitative PCR was used to determine the expression levels of vv-gtol at different growth periods of Volvariella volvacea., Results: The full sequence of vv-gtol covered 2083 bp, containing 11 exons and 10 introns, and encoded a protein with 356 amino acids. 5'UTR was 305 bp which contains one intron region, and 3'UTR was 86bp. Two intron retentions could be recognized during RNA processing, and the transcripts formed by the intron retention could not translate the correct conservative functional domains. The full-length of vv-gtol had more than 50 accurate positioning genome sequencing reads, suggesting that genome sequencing and assembly results are accurate and reliable. The phylogenetic tree showed that GTO1 of Volvariella volvacea belonged to the subclass I of the Omega class of glutathione S-transferase superfamily, and had the closest relationship with GTO1 and GTO2 in Phanerochaete chrysosporium. The analysis of digital gene expression profiling, fluorescence quantitative PCR and proteomics showed that vv-gtol had the highest expression level in the heterokaryotic hyphae., Conclusions: This is the first time to obtain a gene encoding glutathione S-transferase from Volvariella volvacea which belongs to Omega class. Our study showed that the gene may play an important role during the special biological functions of heterokaryotic hyphae. This study also suggested that Volvariella volvacea heterokaryotic hyphae in H1521 had stronger resistance ability than other samples. In addition, vv-gto1 could form different alternative splicesome to regulate gene transcription and translation, and ultimately affect the function of the protein.