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36 results on '"Guo, Yanghao"'

35. [Study on effect of Vitreoscilla hemoglobin gene expression on growth and metabolism of Streptomyces aureofaciens].

Author

Meng C, Ye Q, Qiu L, Shi X, Su W, and Guo Y

Subjects
Bacterial Proteins genetics, Gene Expression, Hemoglobins genetics, Oxygen administration & dosage, Promoter Regions, Genetic, Streptomyces aureofaciens metabolism, Tetracycline Resistance, Truncated Hemoglobins, Bacterial Proteins biosynthesis, Chlortetracycline biosynthesis, Genes, Bacterial, Hemoglobins biosynthesis, Streptomyces aureofaciens growth & development
Abstract

Vitreoscilla hemoglobin gene was expressed in S. aureofaciens through promoter of tetracycline resistance gene. Characteristics of S. aureofaciens growth and metabolism were studied in 1 m3 fermenter. In high dissolved oxygen concentrations, expression of hemoglobin gene had little effect on growth and metabolism of S. aureofaciens, and there were no obvious differences between engineering strain and control. The chlortetracycline of engineering strain was 22905 u/mL and of control was 22896 u/mL respectively. Under conditions of low dissolved oxygen, expression of hemoglobin enhanced growth, maintenance of energetic mycelium configuration and chlortetracycline yield of S. aureofaciens: the mycelium concentrations of engineering strain were more about 5%-10% and yield was more 11.4% than control.

Published
2002

36. [Cloning and expression of Vitreoscilla hemoglobin in Streptomyces aureofaciens].

Author

Meng C, Ye Q, Shi X, Qiu L, Song S, and Guo Y

Subjects
Bacterial Proteins biosynthesis, Cloning, Molecular, Escherichia coli genetics, Genetic Vectors, Hemoglobins biosynthesis, Plasmids, Promoter Regions, Genetic, Streptomyces aureofaciens metabolism, Tetracycline Resistance genetics, Truncated Hemoglobins, Bacterial Proteins genetics, Chlortetracycline biosynthesis, Genes, Bacterial, Hemoglobins genetics, Streptomyces aureofaciens genetics
Abstract

Vitreoscilla hemoglobin Gene was cloned in Streptomyces aureofaciens through E. coli-Streptomyces shuttle plasmids constructed by both pIJ699-pUC19(vhb) and pIJ702-pBR322(vhb). Under low dissolved oxygen conditions, expression of hemoglobin enhanced CTC yield of engineering strain more about 40%-60% that that of control by improving oxygen transmission in cells. Hemoglobin expression by Promoter of tetracycline resistance gene was more effective for oxygen transmission than that by its native promoter regulated by dissolved oxygen concentrations in environments of locally low concentrations of dissolved oxygen.

Published
2002

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