249 results on '"Guo-Wei Zhang"'
Search Results
2. Pathogenic genes of primary open angle glaucoma in a Chinese family
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Juan-Juan Xie, Ying Chen, Guo-Wei Zhang, Hai-Yue Cui, Meng-Yao Hao, Shao-Wen Wang, Zhen Jia, Chun-Long Xu, and Hong Lu
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primary open angle glaucoma ,ltbp2 gene ,gene mutation ,whole exon sequencing ,inheritance ,Ophthalmology ,RE1-994 - Abstract
AIM: To identify the pathogenic gene in a family with primary open angle glaucoma(POAG)from Nantong, Jiang Province, and to analyze its clinical phenotype and pathogenic mechanism.METHOD: A POAG pedigree was reviewed and recruited from January 2020 to December 2020, which spans 5 generations, with 33 people in total. A total of 13 family members were enrolled in our study, of whom 4 members were diagnosed with POAG, 1 with ocular hypertension(OHT), and the other 8 members were unaffected. Detailed medical history was collected and a comprehensive ophthalmic examination was performed. High throughput sequencing was used to screen for possible pathogenic gene, and Sanger sequencing was used to verify candidate pathogenic gene.RESULT: All patients in this family were found to have elevated intraocular pressure(IOP)and diagnosed with glaucoma at a young age, requiring surgical treatments to control the IOP. The highest IOP of proband was up to 55mmHg. A heterozygous mutation(c.1197C>A, p.Phe399Leu)of LTBP2 gene was found in the proband genome by whole exon sequencing(WES). Sanger sequencing verified that the mutation was not isolated from the family disease.CONCLUSION: LTBP2 (c.1197C>A)mutation was not the pathogenic gene of POAG in this family. However, the pathogenic potential of LTBP2 gene in POAG cases is worth studying.
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- 2023
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3. Acinar ATP8b1/LPC pathway promotes macrophage efferocytosis and clearance of inflammation during chronic pancreatitis development
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Wang-jun Yang, Rong-chang Cao, Wang Xiao, Xiao-lou Zhang, Hao Xu, Meng Wang, Zhi-tao Zhou, Huo-ji Chen, Jia Xu, Xue-mei Chen, Jun-ling Zeng, Shu-ji Li, Min Luo, Yan-jiang Han, Xiao-bing Yang, Guo-dong Feng, Yu-heng Lu, Yuan-yuan Ni, Chan-gui Wu, Jun-jie Bai, Zi-qi Yuan, Jin Jin, and Guo-wei Zhang
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Cytology ,QH573-671 - Abstract
Abstract Noninflammatory clearance of dying cells by professional phagocytes, termed efferocytosis, is fundamental in both homeostasis and inflammatory fibrosis disease but has not been confirmed to occur in chronic pancreatitis (CP). Here, we investigated whether efferocytosis constitutes a novel regulatory target in CP and its mechanisms. PRSS1 transgenic (PRSS1 Tg ) mice were treated with caerulein to mimic CP development. Phospholipid metabolite profiling and epigenetic assays were performed with PRSS1 Tg CP models. The potential functions of Atp8b1 in CP model were clarified using Atp8b1-overexpressing adeno-associated virus, immunofluorescence, enzyme-linked immunosorbent assay(ELISA), and lipid metabolomic approaches. ATAC-seq combined with RNA-seq was then used to identify transcription factors binding to the Atp8b1 promoter, and ChIP-qPCR and luciferase assays were used to confirm that the identified transcription factor bound to the Atp8b1 promoter, and to identify the specific binding site. Flow cytometry was performed to analyze the proportion of pancreatic macrophages. Decreased efferocytosis with aggravated inflammation was identified in CP. The lysophosphatidylcholine (LPC) pathway was the most obviously dysregulated phospholipid pathway, and LPC and Atp8b1 expression gradually decreased during CP development. H3K27me3 ChIP-seq showed that increased Atp8b1 promoter methylation led to transcriptional inhibition. Atp8b1 complementation substantially increased the LPC concentration and improved CP outcomes. Bhlha15 was identified as a transcription factor that binds to the Atp8b1 promoter and regulates phospholipid metabolism. Our study indicates that the acinar Atp8b1/LPC pathway acts as an important “find-me” signal for macrophages and plays a protective role in CP, with Atp8b1 transcription promoted by the acinar cell-specific transcription factor Bhlha15. Bhlha15, Atp8b1, and LPC could be clinically translated into valuable therapeutic targets to overcome the limitations of current CP therapies.
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- 2022
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4. Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork
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Miao Wei, Lu-Ming Chen, Ze-Yu Huang, Guo-Wei Zhang, Huai-Jin Guan, and Min Ji
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dexamethasone ,trabecular meshwork cells ,steroid-induced glaucoma ,differentially expressed genes ,protein-protein interaction ,Ophthalmology ,RE1-994 - Abstract
AIM: To investigate potential gene changes in trabecular meshwork (TM) induced by dexamethasone (DEX) in steroid-induced glaucoma (SIG). METHODS: The expression data of 24 cases from a public functional genomics data were sorted to identify the mechanisms of action of DEX on the TM. The relationships of the differentially expressed genes (DEGs) were enriched using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In addition, the hub genes were screened by the Search Tool for the Retrieval of Interacting Genes Database (STRING) and Cytoscape tools. Finally, human TM cells (HTMCs) were treated with DEX to preliminarily explore the function of hub genes. RESULTS: Totally 47 DEGs, including 21 downregulated and 26 upregulated genes were identified. The primary enriched results of the DEGs consisted of inflammatory response, extracellular matrix (ECM), negative regulation of cell proliferation, TNF signalling pathway and the regulation of tryptophan channels by inflammatory mediators. Subsequently, pro-melanin-enriched hormone (PMCH) and Bradykinin B1 receptor (BDKRB1) were screened as hub genes. It is verified in GSE37474 data set. Western blot and quantitative real-time polymerase chain reaction (qPCR) results showed that protein and RNA expression levels of BDKRB1 were significantly decreased after DEX treatment, while PMCH was not significantly changed. CONCLUSION: BDKRB1 may be a key gene involved in SIG onset, providing a suitable therapeutic target for improving the prognosis of SIG patients.
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- 2022
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5. St13 protects against disordered acinar cell arachidonic acid pathway in chronic pancreatitis
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Rong-chang Cao, Wan-jun Yang, Wang Xiao, Lei Zhou, Jie-hui Tan, Meng Wang, Zhi-tao Zhou, Huo-ji Chen, Jia Xu, Xue-mei Chen, Yang-chen Jin, Jia-yu Lin, Jun-ling Zeng, Shu-ji Li, Min Luo, Guo-dong Hu, Jin Jin, Xiao-bing Yang, Da Huo, Jie Zhou, and Guo-wei Zhang
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PRSS1 ,Pancreatitis ,St13 ,Sdf2l1 ,Lipid metabolism ,PET/CT ,Medicine - Abstract
Abstract Background Early diagnosis and treatment of chronic pancreatitis (CP) are limited. In this study, St13, a co-chaperone protein, was investigated whether it constituted a novel regulatory target in CP. Meanwhile, we evaluated the value of micro-PET/CT in the early diagnosis of CP. Methods Data from healthy control individuals and patients with alcoholic CP (ACP) or non-ACP (nACP) were analysed. PRSS1 transgenic mice (PRSS1 Tg ) were treated with ethanol or caerulein to mimic the development of ACP or nACP, respectively. Pancreatic lipid metabolite profiling was performed in human and PRSS1 Tg model mice. The potential functions of St13 were investigated by crossing PRSS1 Tg mice with St13−/− mice via immunoprecipitation and lipid metabolomics. Micro-PET/CT was performed to evaluate pancreatic morphology and fibrosis in CP model. Results The arachidonic acid (AA) pathway ranked the most commonly dysregulated lipid pathway in ACP and nACP in human and mice. Knockout of St13 exacerbated fatty replacement and fibrosis in CP model. Sdf2l1 was identified as a binding partner of St13 as it stabilizes the IRE1α-XBP1s signalling pathway, which regulates COX-2, an important component in AA metabolism. Micro-PET/CT with 68Ga-FAPI-04 was useful for evaluating pancreatic morphology and fibrosis in CP model mice 2 weeks after modelling. Conclusion St13 is functionally activated in acinar cells and protects against the cellular characteristics of CP by binding Sdf2l1, regulating AA pathway. 68Ga-FAPI-04 PET/CT may be a very valuable approach for the early diagnosis of CP. These findings thus provide novel insights into both diagnosis and treatment of CP.
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- 2022
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6. The effect of posterior lumbar dynamic fixation and intervertebral fusion on paraspinal muscles
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Geng-Xiong Lin, Yan-Ming Ma, Yong-Chun Xiao, Dian Xiang, Jian-Xian Luo, Guo-Wei Zhang, Zhi-Sheng Ji, and Hong-Sheng Lin
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K-rod ,Dynamic internal fixation ,PLIF ,Lumbar degenerative disease ,Paraspinal muscles atrophy ,Fatty infiltration ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background The aim of this study was to analyze the effect of unilateral K-rod dynamic internal fixation on paraspinal muscles for lumbar degenerative diseases. Methods This study retrospectively collected 52 patients who underwent lumbar surgery with the K-rod group or PLIF. The operation time, intraoperative blood loss, postoperative drainage volume, postoperative exercise time were compared in the two groups. The visual analog scale (VAS) score and the oswestry dysfunction index (ODI) were employed to evaluate the clinical outcomes. The functional cross-sectional area (FCSA) of the paraspinal muscles and paraspinal muscles fat infiltration were measured to assess on the paraspinal muscles. Results As compared with the PLIF group, the operation time, the postoperative time in the field, and the average postoperative hospital stay in the K-rod internal fixation group were significantly shortened. At the last follow-up, both the groups showed significant improvement in the VAS score and ODI. The FCSA atrophy of the upper and lower adjacent segments (UAS and LAS) of the K-rod internal group was significantly less than that of the PLIF group. The extent of increase in the fatty infiltration of the paraspinal muscles in the K-rod group was significantly lesser than that in the PLIF group. The postoperative low back pain of the two groups of patients was significantly positively correlated with the FCSA atrophy. Conclusions As compared to PLIF, the posterior lumbar unilateral K-rod dynamic internal fixation showed significantly lesser paraspinal muscle atrophy and fatty infiltration, which were significantly positively correlated with postoperative low back pain.
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- 2021
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7. Effect of age on reversible lens opacity and Na+-K+-ATPase expression induced by chloral hydrate in mice
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Bai Qin, Jun-Fang Zhang, Mei Yang, Liu-Ping Wu, Li-Hua Kang, Guo-Wei Zhang, Hai-Hong Shi, and Huai-Jin Guan
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lens opacity ,chloral hydrate ,age ,na+-k+-atpase ,mice ,Ophthalmology ,RE1-994 - Abstract
AIM: To investigate the effect of age on the expression of Na+-K+-ATPase and acute reversible lens opacification induced by chloral hydrate in mice. METHODS: Acute reversible lens opacification was induced by intraperitoneal injection of 4% chloral hydrate(400mg/kg)in 3-month-old(young group)and 24-month-old(old group)C57BL/6 mice. The lens opacification was graded at 10, 20, 30, 45, 60, 90, 120 and 150min after chloral hydrate injection. The histopathological changes of lens were observed by hematoxylin eosin staining, and the expression of Na+-K+-ATPase in lens was detected by immunohistochemistry. RESULTS: The development of lens opacity is similar in young and old mice after chloral hydrate injection. The lens opacification in the young group appeared earlier, thicker and lasted longer than the old group. HE staining showed that many vesicles appeared in the cortex below lens epithelial cells(LECs), and the structure of superficial lens fiber cells were disordered after chloral hydrate injection. Immunohistochemical staining showed that the expression of Na+-K+-ATPase was positive in LECs and fibers. The expression of Na+-K+-ATPase in LECs were weak before chloral hydrate injection and up-regulated 45min after chloral hydrate injection in young and old groups. The up-regulation of Na+-K+-ATPase was stronger in the old group than in the young group. CONCLUSION: Age may play a role in the acute reversible lens opacification induced by chloral hydrate in mice. The expression of Na+-K+-ATPase is involved in lens opacity induced by chloral hydrate.
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- 2021
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8. Research progress on the exosomes in ophthalmic diseases
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Jia-Wei Luo, Guo-Wei Zhang, Li-Hua Kang, and Huai-Jin Guan
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exosomes ,ophthalmic diseases ,molecular pathology ,intercellular communication ,research progress ,Ophthalmology ,RE1-994 - Abstract
Exosomes are extracellular vesicles of sizes ranging from 50-150nm in diameter. Exosomes can deliver bioactive molecules(e.g. proteins, lipids, DNA, microRNA, etc.)into target cells which play an important role in cell-cell communication. Researches demonstrated that exosomes mediated cell-cell communication can impact cell apoptosis, invasion, migration, immune response and oxidative repair ability of recipient cells. Recently, researches on exosomes developed rapidly in the field of ophthalmology. This review summarized the latest research progress of exosomes in ophthalmic diseases.
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- 2021
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9. Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching
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Zhi-Sheng Ji, Jian-Ping Li, Chao-Hua Fu, Jian-Xian Luo, Hua Yang, Guo-Wei Zhang, Wutian Wu, and Hong-Sheng Lin
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collapsin response mediator protein 3 ,liquid chromatography-mass spectrometry ,microtubule ,neurite growth ,protein interactions ,proteomics ,spastin ,spinal cord injury ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Cytoskeletal microtubule rearrangement and movement are crucial in the repair of spinal cord injury. Spastin plays an important role in the regulation of microtubule severing. Both spastin and collapsin response mediator proteins can regulate neurite growth and branching; however, whether spastin interacts with collapsin response mediator protein 3 (CRMP3) during this process remains unclear, as is the mechanism by which CRMP3 participates in the repair of spinal cord injury. In this study, we used a proteomics approach to identify key proteins associated with spinal cord injury repair. We then employed liquid chromatography-mass spectrometry to identify proteins that were able to interact with glutathione S-transferase-spastin. Then, co-immunoprecipitation and staining approaches were used to evaluate potential interactions between spastin and CRMP3. Finally, we co-transfected primary hippocampal neurons with CRMP3 and spastin to evaluate their role in neurite outgrowth. Mass spectrometry identified the role of CRMP3 in the spinal cord injury repair process. Liquid chromatography-mass spectrometry pulldown assays identified three CRMP3 peptides that were able to interact with spastin. CRMP3 and spastin were co-expressed in the spinal cord and were able to interact with one another in vitro and in vivo. Lastly, CRMP3 overexpression was able to enhance the ability of spastin to promote neurite growth and branching. Therefore, our results confirm that spastin and CRMP3 play roles in spinal cord injury repair by regulating neurite growth and branching. These proteins may therefore be novel targets for spinal cord injury repair. The Institutional Animal Care and Use Committee of Jinan University, China approved this study (approval No. IACUS-20181008-03) on October 8, 2018.
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- 2021
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10. Roles and Clinical Significances of ATF6, EMC6, and APAF1 in Prognosis of Pancreatic Cancer
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Wang Xiao, Rong-Chang Cao, Wan-Jun Yang, Jie-Hui Tan, Ruo-Qi Liu, He-Ping Kan, Lei Zhou, Na Zhang, Zhi-Ye Chen, Xue-Mei Chen, Jia Xu, Guo-Wei Zhang, and Peng Shen
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pancreatic cancer ,endoplasmic reticulum stress (ER stress) ,apoptosis ,ATF6 ,EMC6 ,Apaf1 ,Genetics ,QH426-470 - Abstract
Background: Pancreatic cancer (PC) is prevalent among malignant tumors with poor prognosis and lacks efficient therapeutic strategies. Endoplasmic reticulum (ER) stress and apoptosis are associated with chronic inflammation and cancer progression. However, the prognostic value of ER stress-related, and apoptosis-related genes in PC remains to be further elucidated. Our study aimed at confirming the prognostic values of the ER stress-related genes, ATF6, EMC6, XBP1, and CHOP, and the apoptosis-related gene, APAF1, in PC patients.Methods: Gene Expression Profiling Interactive Analysis 2 (GEPIA2) was used to evaluate prognosis value of ATF6, EMC6, XBP1, CHOP, and APAF1 in PC. Clinical data from 69 PC patients were retrospectively analyzed. Immunohistochemistry, Western blotting, and qRT-PCR were used for the assessment of gene or protein expression. The cell counting kit-8 (CCK-8) and the Transwell invasion assays were, respectively, used for the assessment of the proliferative and invasive abilities of PC cells. The prognostic values of ATF6, XBP1, CHOP, EMC6, and APAF1 in PC patients were evaluated using Kaplan–Meier and Cox regression analyses.Results: XBP1 and CHOP expressions were not associated with PC recurrence-free survival (RFS), overall survival (OS) and disease-specific survival (DSS). ATF6 upregulation and EMC6 and APAF1 downregulations significantly correlated with the poor RFS, OS, and DSS of PC patients. ATF6 promoted PC cell proliferation and invasion, while EMC6 and APAF1 inhibited these events.Conclusion: ATF6 upregulation and EMC6 and APAF1 downregulations may be valid indicators of poor prognosis of PC patients. Moreover, ATF6, EMC6, and APAF1 may constitute potential therapeutic targets in PC patients.
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- 2022
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11. Analysis of clinical effect and radiographic outcomes of Isobar TTL system for two-segment lumbar degenerative disease: a retrospective study
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Zhi-Sheng Ji, Hua Yang, Yu-Hao Yang, Shao-Jin Li, Jian-Xian Luo, Guo-Wei Zhang, and Hong-Sheng Lin
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Isobar TTL ,Dynamic stabilization system ,Two-segment ,Lumbar degenerative disease ,Selective fusion ,Surgery ,RD1-811 - Abstract
Abstract Background Nonfusion fixation is an effective way to treat lumbar degeneration. In the present study, we analyzed the clinical effects and radiographic outcomes of the Isobar TTL system used to treat two-segment lumbar degenerative disease. Method Forty-one patients diagnosed with two-segment lumbar degenerative disease underwent surgical implantation of the Isobar TTL dynamic stabilization system (n = 20) or a rigid system (n = 21) from January 2013 to June 2017. The mean follow-up time was 23.6 (range 15–37) months. Clinical results were evaluated with the Oswestry Disability Index (ODI), modified Macnab criteria, and the visual analog score (VAS). Radiographic evaluations included the height of the intervertebral space and the range of motion (ROM) of the treated and adjacent segments. The intervertebral disc signal was classified using the modified Pfirrmann grading system and the University of California at Los Angeles (UCLA) system. Results The clinical results, including the ODI and VAS, showed that there was significant improvement in the two groups after implantation and that the difference between the two groups was not significant. In addition, the clinical efficacy indicated by the modified Macnab criteria for the two groups was similar. Radiological outcomes included the height of the intervertebral space, lumbar mobility, and intervertebral disc signal. The height of the intervertebral space of the upper adjacent segment L2/3 in the rigid group was significantly lower than that in the Isobar TTL group at the last follow-up. Furthermore, the number of ROMs of the fixed-segment L3/4 in the Isobar TTL group was significantly less than that before implantation, suggesting that the fixed-segment ROMs in the Isobar TTL group were limited. In addition, the ROM of the upper adjacent segment L2/3 in the last follow-up of the rigid group increased significantly, while that of the Isobar TTL group did not change after implantation. Finally, the incidence of adjacent-segment degeneration (ASD) was significantly greater in the rigid group than in the Isobar TTL group according to the UCLA system. Conclusion The Isobar TTL system can be clinically effective for treating two-segment lumbar degenerative disease. Compared with rigid fixation, the Isobar TTL system yielded better radiographic outcomes and maintained the mobility of the treated segments with less impact on the proximal adjacent segment.
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- 2020
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12. Correction: Acinar ATP8b1/LPC pathway promotes macrophage efferocytosis and clearance of inflammation during chronic pancreatitis development
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Wan-jun Yang, Rong-chang Cao, Wang Xiao, Xiao-lou Zhang, Hao Xu, Meng Wang, Zhi-tao Zhou, Huo-ji Chen, Jia Xu, Xue-mei Chen, Jun-ling Zeng, Shu-ji Li, Min Luo, Yan-jiang Han, Xiao-bing Yang, Guo-dong Feng, Yu-heng Lu, Yuan-yuan Ni, Chan-gui Wu, Jun-jie Bai, Zi-qi Yuan, Jin Jin, and Guo-wei Zhang
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Cytology ,QH573-671 - Published
- 2022
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13. Density functional theory investigation on the stability, adhesion strength, tensile properties and fracture behavior of γ-Fe/Cu heterogeneous nucleation interface
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Ming-Jie Wang, Guo-Wei Zhang, Hong Xu, Ying Dong, Feng-Er Sun, and Yi-Zheng Fu
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Density functional theory ,Adhesion energy ,Fracture behavior ,γ-Fe (110)/Cu (110) interface ,Heterogeneous nucleation ,Physics ,QC1-999 - Abstract
To theoretically study the stability and the heterogeneous nucleation characteristics of γ-Fe/Cu interface in steel/copper bimetallic, the six surface models and seven interface models were investigated through density functional theory. Besides, the interface stability, adhesion energy, electronic properties, tensile process and fracture behavior of three kinds typical Fe/Cu interface model were calculated by first-principles calculations. Simultaneously, the mechanism on heterogeneous nucleation of pure Fe on Cu-based alloy was revealed. The results indicate that the bramfitt’s lattice mismatches of all the three Fe/Cu interfaces were both 4.11%, and all the Cu surfaces and the Fe surfaces were trends to converge, when the number of layers were 9 and 7, respectively. Among three kinds Fe/Cu interfaces with HCP structure, the Fe (110)/Cu (110) interface shown higher strength of metallic bonds and stable ionic properties. When the strain is 37%, the maximum value of the tensile stress is about 20.3GPa and the electron density between Fe and Cu atoms drop to disappear. The calculated adhesion work of Fe (110)/Cu (110) interface with HCP structure is 1.88 J/m2, is higher than that between Cu/Cu melt (1.78 J/m2), which proved that the Fe particles can promote heterogeneous nucleation of Cu atoms and then account for the grain refinement of Cu-based alloy.
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- 2021
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14. SUMOylation of spastin promotes the internalization of GluA1 and regulates dendritic spine morphology by targeting microtubule dynamics
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Zhi-Sheng Ji, Qiu-Ling Liu, Ji-feng Zhang, Yu-Hao Yang, Jiong Li, Guo-Wei Zhang, Ming-Hui Tan, Hong-Sheng Lin, and Guo-Qing Guo
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SUMOylation ,Spastin ,Dendritic spine ,Microtubule dynamics ,GluA1 internalization ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Dendritic spines are specialized structures involved in neuronal processes on which excitatory synaptic contact occurs. The microtubule cytoskeleton is vital for maintaining spine morphology and mature synapses. Spastin is related to microtubule-severing proteases and is involved in synaptic bouton formation. However, it is not yet known if spastin can be modified by Small Ubiquitin-like Modifier (SUMO) or how this modification regulates dendritic spines. Spastin was shown to be SUMOylated at K427, and its deSUMOylation promoted microtubule stability. In addition, SUMOylation of spastin was shown to affect signalling pathways associated with long term synaptic depression. SUMOylated spastin promoted the development of dendrites and dendritic spines. Moreover, SUMOylated spastin regulated endocytosis and affected the transport of the AMPA receptor, GluA1. Our findings suggest that SUMOylation of spastin promotes GluA1 internalization and regulates dendritic spine morphology through targeting of microtubule dynamics.
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- 2020
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15. Identification of risk factors for pancreatic pseudocysts formation, intervention and recurrence: a 15-year retrospective analysis in a tertiary hospital in China
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Jie-hui Tan, Lei Zhou, Rong-chang Cao, and Guo-wei Zhang
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Pancreatic pseudocyst ,Alcoholic ,Pancreatitis ,Outcome ,Intervention ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Abstract Background Pancreatic pseudocyst (PPC) is a common complication of acute and chronic pancreatitis. To our knowledge no study has systematically reported the risk factors for the formation, intervention and recurrence of PPC. Therefore, the present study aimed to investigate the potential risk factors for PPC, with regards to its formation, intervention and recurrence. Methods A database containing 5106 pancreatitis patients was retrospectively analyzed. As a result, a total of 4379 eligible patients were identified and divided into 2 groups: PPC group (group A, n = 759) and non-PPC group (group B, n = 3620). The PPC group was subdivided into 2 groups: intervention PPC (group C, n = 347) and resolution PPC (group D, n = 412). The differences in surgical complication and recurrence rates were compared among 347 PPC patients receiving different interventions, including surgical, endoscopic and percutaneous drainages. Furthermore, group C was subdivided into 2 groups: recurrent PPC (group E, n = 34) and non-recurrent PPC (group F, n = 313). All possible risk factors for PPC formation, intervention and recurrence were determined by multivariate regression analysis. Results In this study, PPC was developed in 17.3% (759/4379) of pancreatitis patients. The significant risk factors for PPC formation included alcoholic pancreatitis (OR, 6.332; 95% CI, 2.164–11.628; p = 0.031), chronic pancreatitis (CP) (OR, 5.822; 95% CI, 1.921–10.723; p = 0.006) and infected pancreatic necrosis (OR, 4.253; 95% CI, 3.574–7.339; p = 0.021). Meanwhile, the significant risk factors of PPC patients who received intervention were alcoholic pancreatitis (OR, 7.634; 95% CI, 2.125–13.558; p = 0.016), size over 6 cm (OR, 8.834; 95% CI, 2.017–16.649; p = 0.002) and CP (OR, 4.782; 95% CI, 1.897–10.173; p = 0.038). In addition, the recurrence rate in PPC patients treated with percutaneous drainage was found to be the highest (16.3%) among the three intervention groups. Furthermore, percutaneous drainage was the only risk factor of PPC recurrence (OR, 7.812; 95% CI, 3.109–23.072; p = 0.013) identified from this retrospective cohort study. Conclusions Alcoholic pancreatitis and CP are the main risk factors for PPC formation and intervention, but not PPC recurrence. A higher recurrence rate is found in PPC patients treated with percutaneous drainage, as compared to endoscopic and surgical interventions.
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- 2018
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16. Epidemiological survey of age-related macular degeneration in population aged ≥50 years in Funing county, Jiangsu
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Yu Guan, Mei Yang, Li-Hua Kang, Guo-Wei Zhang, and Huai-Jin Guan
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vision ,age-related macular degeneration ,prevalence ,Ophthalmology ,RE1-994 - Abstract
AIM: To investigate the prevalence of age-related macular degeneration(ARMD)among people aged 50 years and above in Funing county, Jiangsu province. METHODS: Survey research. Random cluster sampling was used in selecting individuals aged ≥50 years in 30 clusters in Funing county. Proportions were compared by using the χ2 test and the means compared by using the t-test. Logistic regression was used to detect possible factors of ARMD such as age and gender. RESULTS: A total of 6 145 persons aged 50 years were enumerated and 5 947(96.78%)participants were received visual acuity test and eye examination. The prevalence of ARMD was 7.53%, with a total of 448 individuals(633 eyes). The prevalence of blindness and visual impairment for presenting visual acuity were 4.13% and 11.96%, respectively. The prevalence of blindness and visual impairment for presenting visual acuity were 4.45% and 7.79%, respectively. Older were significant risk factors of ARMD(OR=1.01, P=0.04). CONCLUSION: The prevalence of ARMD was higher among people aged 50 years and above in Funing county, Jiangsu province. ARMD is one of the leading cause of visual impairment.
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- 2018
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17. Research on Hydroelastic Response of an FMRC Hexagon Enclosed Platform
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Wei-Qin Liu, Luo-Nan Xiong, Guo-Wei Zhang, Meng Yang, Wei-Guo Wu, and Xue-Min Song
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hexagon enclosed platform ,FMRC ,hydroelastic ,load ,stress ,frequency-domain ,Mathematics ,QA1-939 - Abstract
The numerical hydroelastic method is used to study the structural response of a hexagon enclosed platform (HEP) of flexible module rigid connector (FMRC) structure that can provide life accommodation, ship berthing and marine supply for ships sailing in the deep ocean. Six trapezoidal floating structures constitute the HEP structure so that it is a symmetrical very large floating structure (VLFS). The HEP has the characteristics of large area and small depth, so its hydroelastic response is significant. Therefore, this paper studies the structural responses of a hexagon enclosed platform of FMRC structure in waves by means of a 3D potential-flow hydroelastic method based on modal superposition. Numerical models, including the hydrodynamic model, wet surface model and finite element method (FEM) model, are established, a rigid connection is simulated by many-point-contraction (MPC) and the number of wave cases is determined. The load and structural response of HEP are obtained and analyzed in all wave cases, and frequency-domain hydroelastic calculation and time-domain hydroelastic calculation are carried out. After obtaining a number of response amplitude operators (RAOs) for stress and time-domain stress histories, the mechanism of the HEP structure is compared and analyzed. This study is used to guide engineering design for enclosed-type ocean platforms.
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- 2021
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18. Preparation of CO2/N2 cryogenic slurry and its pipeline flow characteristics
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Xin-hai ZHANG, Kai DOU, Guo-wei ZHANG, Xun-guang LI, Wang-shou CHENG, and Hui ZHU
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Environmental sciences ,GE1-350 - Abstract
It was proposed to use CO2/N2 to effectively prevent and control Class A solid spontaneous fires such as coal. The preparation process of CO2/N2 cryogenic slurry pipeline and its pipeline flow characteristics were studied. The temperature field, pressure field and phase change characteristic changed during the transportation of the CO2/N2 mixture in the pipeline are analyzed by numerical simulation. The results showed that: the fluid in the pipeline exchanged heat and started to contact at a position of 0.1m in the pipeline. The temperature first rapidly dropped to the lowest temperature of 103K, and then the fluids in the range of 0.1m to 0.3m were fully mixed, and the temperature rised to 164K. The temperature was consistent with the equilibrium state in the pipe, and the temperature was gentle and stable; the pressure in the pipe was on the whole downward trend, and each position along the main pipe produced a positive pressure difference relative to the end of the pipe. The closer to the mixing place, the greater the corresponding pressure difference; During the mixing process, LN2 was acted as a cold source to sublime CO2 to form dry ice particles. And through the cryogenic fluid mixing experiment, the temperature distribution in the pipeline, range of the dry ice particle generation position, and the dry ice particle generation phenomenon was measured. The results showed that the mixing was completed within 0.8 m of the pipeline, and the temperature in the tube rised to 168K and remain stable. The data were basically consistent. And dry ice particles generated at the temperature of 194.5K, and a diffuse gas cloud formed at the outlet with the momentum of the jet.
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- 2021
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19. The Application of GGE Biplot Analysis for Evaluat ng Test Locations and Mega-Environment Investigation of Cotton Regional Trials
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Nai-yin XU, Michel Fok, Guo-wei ZHANG, Jian LI, and Zhi-guo ZHOU
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cotton ,multi-environmental trial ,GGE biplot ,test location ,mega-environment ,Agriculture (General) ,S1-972 - Abstract
In the process to the marketing of cultivars, identification of superior test locations within multi-environment variety trial schemes is of critical relevance. It is relevant to breeding organizations as well as to governmental organizations in charge of cultivar registration. Where competition among breeding companies exists, effective and fair multi-environment variety trials are of utmost importance to motivate investment in breeding. The objective of this study was to use genotype main effect plus genotype by environment interaction (GGE) biplot analysis to evaluate test locations in terms of discrimination ability, representativeness and desirability, and to investigate the presence of multiple mega-environments in cotton production in the Yangtze River Valley (YaRV), China. Four traits (cotton lint yield, fiber length, lint breaking tenacity, micronaire) and two composite selection indices were considered. It was found that the assumption of a single mega-environment in the YaRV for cotton production does not hold. The YaRV consists of three cotton mega-environments: a main one represented by 11 locations and two minor ones represented by two test locations each. This demands that the strategy of cotton variety registration or recommendation must be adjusted. GGE biplot analysis has also led to the identification of test location superior for cotton variety evaluation. Although test location desirable for selecting different traits varied greatly, Jinzhou, Hubei Province, China, was found to be desirable for selecting for all traits considered while Jianyang, Sichuan Province, China, was found to be desirable for none.
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- 2014
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20. Polarization-insensitive quantum key distribution using planar lightwave circuit chips.
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Guo-Wei Zhang, Wei Chen 0132, Guan-Jie Fan-Yuan, Li Zhang, Fang-Xiang Wang, Shuang Wang, Zhen-Qiang Yin, De-Yong He, Wen Liu, Jun-Ming An, Guang-Can Guo, and Zheng-Fu Han
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- 2022
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21. Intelligent measuring device and method for large grinding wheel size
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Xiao-Wei ZHU, Lin ZHANG, Hai-Qi Xi, Zhe-Hui LI, Fei Liu, Guo-Wei ZHANG, Chong GAO, Ben YANG, and Zhifeng Zhang
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- 2023
22. Penetrance of MYOC gene mutation in primary open-angle glaucoma: A systematic review and meta-analysis
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Juan-Juan Xie, Guo-Wei Zhang, Hai-Yue Cui, Na Li, Xing-Xing Liu, Meng-Yao Hao, Shao-Wen Wang, and Hong Lu
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Ophthalmology ,genetic structures ,Pediatrics, Perinatology and Child Health ,eye diseases ,Genetics (clinical) - Abstract
To investigate the penetrance of MYOC gene mutation in primary open-angle glaucoma (POAG) through systematic review and meta-analysis. To explore the factors affecting the penetrance of MYOC and provide evidence-based medical evidence for clinical work. We searched all studies that reported the penetrance of MYOC mutation in PubMed, Embase, Web of Science, and Chinese databases including Wanfang, CNKI (China National Knowledge Infrastructure), and CBM (China Bio-Med). Random effects meta-analysis was conducted to estimate the penetrance of MYOC mutation in POAG. Fifty-two studies were included in this analysis after screening. Meta-analysis of the penetrance of MYOC mutation showed that the penetrance of MYOC mutation in POAG was 60% (95% CI: 51.0% to 68.0%) and the penetrance of MYOC mutation in POAG and suspected POAG was 68% (95% CI: 60.0% to 75.0%). The penetrance of MYOC mutation increases with age. Among Caucasians, Asians, and Africans, the penetrance of MYOC mutation in POAG was 55%, 71%, 54%, respectively, and the penetrance of MYOC mutation in POAG and suspected POAG was 64%, 83%, and 57%, respectively. Besides, the penetrance of different MYOC mutation sites was significantly discrepant. The penetrance of MYOC mutation in POAG ranged from 10.3% to 100% depending on the mutation sites. Some MYOC mutation sites have a certain population specificity, which is only pathogenic in Caucasians or Asians. The penetrance of MYOC mutation in POAG showed significant differences due to different mutation sites. The penetrance increased with the accrescent of age. Ethnic difference was an important factor affecting the penetrance of MYOC mutation. Knowing the rules and influencing factors of the penetrance of MYOC mutations is significant for the assessment of the risk of POAG in carriers with the MYOC mutation.
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- 2022
23. Induced-photorefractive attack against Quantum Key Distribution
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Peng Ye, Wei Chen, Guo-Wei Zhang, Feng-Yu Lu, Fang-Xiang Wang, Guan-Zhong Huang, Shuang Wang, De-Yong He, Zhen-Qiang Yin, Guang-Can Guo, and Zheng-Fu Han
- Subjects
Quantum Physics ,General Physics and Astronomy ,FOS: Physical sciences ,Quantum Physics (quant-ph) - Abstract
Lithium niobate (LiNbO3, LN) devices play critical roles in quantum information processing. However, for special applications like quantum key distribution (QKD), the characteristics of materials and devices and their impact on practical systems must be intensively inquired. For the first time, we reveal that the photorefractive effect in LN can be utilized as a potential loophole to carry out malicious attacks by the eavesdroppers. We take a commercial LN-based variable optical attenuator as an example to demonstrate the method we named Induced-photorefractive attack (IPA) and propose two techniques to enable controllable attacks. Our results show that eavesdroppers can fulfill an efficient source-side attack by injecting an optimized irradiation beam with only several nanowatts, which is realistic when accessing commercial fiber channels. These measure and techniques can be employed for all individual and on-chip LN devices and initially explored a new security branch for system design and standardization of real-life QKD.
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- 2023
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24. Short‐term creep properties and creep model of wood‐plastic composites
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Fei Xi, Yang Wei, Longlong Zhao, and Guo Wei Zhang
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Materials science ,Polymers and Plastics ,Creep ,Materials Chemistry ,Ceramics and Composites ,General Chemistry ,Composite material ,Term (time) - Published
- 2021
25. Dynamic Nomogram for predicting the severity of acute pancreatitis in adults and Decision Curve analysis: a single-center retrospective study
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Hao Xu, Xing-da Xu, Jie-hui Tan, Wang-jun Yang, Wang Xiao, Xiao-lou Zhang, Si-yun Zhang, Qun He, Xiao-kang Zhang, Jian-ping Qian, and Guo-wei Zhang
- Abstract
Objective Acute pancreatitis (AP) is a common and unpredictable disease. Severity stratification and prognostic prediction play an important role in reducing the mortality of AP and improving its prognosis in the early stage. We aimed to establish a clinical prediction model by logistic regression analysis visualized by nomogram. Methods A total of 497 patients with AP in the Nanfang Hospital, Southern Medical University between January 2015 and January 2018 were retrospectively collected. 497 patients were randomly (ratio = 7:3) divided into the training cohort (N = 347) and the validation cohort (N = 147), and both cohorts were divided into non-severe acute pancreatitis (non-SAP) and SAP groups. Univariate and multivariate logistic regression analysis were used to identify the factors associated with the severity of AP. Based on multivariate logistic regression analysis, nomogram was established and validated. The performance, discrimination, and calibration of nomogram were conducted. Decision curve analysis was used to evaluate the net benefits and clinical usefulness of the prediction model. Result Multivariate logistic regression analysis showed that alcohol use, hyperlipidemia, gender, hypertension, ionized serum calcium and serum albumin were independent risk factors for SAP. The individualized nomogram showed good discrimination both in the training cohort (area under the receiver operating characteristic curve [AUC], 0.782) and in the validation cohort (AUC, 0.764) with good calibration. Decision curve analysis demonstrated that in terms of clinical usefulness, the nomogram was found to have some clinical values and can be used in clinical practice. Conclusion The proposed nomogram based on easy-to-obtain features are of high efficacy in predicting SAP patients, which may facilitate clinical decision-making.
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- 2022
26. 减库对大豆叶片碳代谢的影响
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Guo-Wei ZHANG, Kai LI, Si-Jia LI, Xiao-Jing WANG, Chang-Qin YANG, and Rui-Xian LIU
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Plant Science ,Agronomy and Crop Science ,Biotechnology - Published
- 2021
27. Transmittance-invariant phase modulator for chip-based quantum key distribution
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Peng Ye, Wei Chen, Ze-Hao Wang, Guo-Wei Zhang, Yu-Yang Ding, Guan-Zhong Huang, Zhen-Qiang Yin, Shuang Wang, De-Yong He, Wen Liu, Guang-Can Guo, and Zheng-Fu Han
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Atomic and Molecular Physics, and Optics - Abstract
In chip-based quantum key distribution (QKD) systems, the non-ideal quantum state preparation due to the imperfect electro-optic phase modulators (EOPM) decreases the secret key rate and introduces potential vulnerabilities. We propose and implement an on-chip transmittance-invariant phase modulator (TIPM) to solve this problem. Simulated and experimental results show that TIPM can eliminate the correlation between phase, intensity, and polarization of quantum states caused by phase-dependent loss. The design can tolerate a significant fabrication mismatch and is universal to multi-material platforms. Furthermore, TIPM increases the modulation depth achievable by EOPMs in standard process design kit (PDK). The proposal of TIPM can improve the practical security and performance of the chip-based QKD systems.
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- 2022
28. Acinar ATP8b1/LPC pathway promotes macrophage efferocytosis and clearance of inflammation during chronic pancreatitis development
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Wan-jun Yang, Rong-chang Cao, Wang Xiao, Xiao-lou Zhang, Hao Xu, Meng Wang, Zhi-tao Zhou, Huo-ji Chen, Jia Xu, Xue-mei Chen, Jun-ling Zeng, Shu-ji Li, Min Luo, Yan-jiang Han, Xiao-bing Yang, Guo-dong Feng, Yu-heng Lu, Yuan-yuan Ni, Chan-gui Wu, Jun-jie Bai, Zi-qi Yuan, Jin Jin, and Guo-wei Zhang
- Subjects
Adenosine Triphosphatases ,Inflammation ,Cancer Research ,Macrophages ,Immunology ,Lysophosphatidylcholines ,Acinar Cells ,Cell Biology ,Histones ,Mice ,Cellular and Molecular Neuroscience ,Pancreatitis, Chronic ,Animals ,Phospholipid Transfer Proteins ,Ceruletide ,Transcription Factors - Abstract
Noninflammatory clearance of dying cells by professional phagocytes, termed efferocytosis, is fundamental in both homeostasis and inflammatory fibrosis disease but has not been confirmed to occur in chronic pancreatitis (CP). Here, we investigated whether efferocytosis constitutes a novel regulatory target in CP and its mechanisms. PRSS1 transgenic (PRSS1Tg) mice were treated with caerulein to mimic CP development. Phospholipid metabolite profiling and epigenetic assays were performed with PRSS1Tg CP models. The potential functions of Atp8b1 in CP model were clarified using Atp8b1-overexpressing adeno-associated virus, immunofluorescence, enzyme-linked immunosorbent assay(ELISA), and lipid metabolomic approaches. ATAC-seq combined with RNA-seq was then used to identify transcription factors binding to the Atp8b1 promoter, and ChIP-qPCR and luciferase assays were used to confirm that the identified transcription factor bound to the Atp8b1 promoter, and to identify the specific binding site. Flow cytometry was performed to analyze the proportion of pancreatic macrophages. Decreased efferocytosis with aggravated inflammation was identified in CP. The lysophosphatidylcholine (LPC) pathway was the most obviously dysregulated phospholipid pathway, and LPC and Atp8b1 expression gradually decreased during CP development. H3K27me3 ChIP-seq showed that increased Atp8b1 promoter methylation led to transcriptional inhibition. Atp8b1 complementation substantially increased the LPC concentration and improved CP outcomes. Bhlha15 was identified as a transcription factor that binds to the Atp8b1 promoter and regulates phospholipid metabolism. Our study indicates that the acinar Atp8b1/LPC pathway acts as an important “find-me” signal for macrophages and plays a protective role in CP, with Atp8b1 transcription promoted by the acinar cell-specific transcription factor Bhlha15. Bhlha15, Atp8b1, and LPC could be clinically translated into valuable therapeutic targets to overcome the limitations of current CP therapies.
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- 2022
29. Temperature development in steel members exposed to localized fire in large enclosure
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Guo-wei, Zhang, Guo-qing, Zhu, and Li-li, Huang
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- 2014
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30. Study on prescription medication mode and mechanism of traditional Chinese medicine in the treatment of noncritical COVID-19 based on data mining.
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Jia-Ming Xiong, Jia-Yu Wang, Yi Liu, Bin Hao, Yue-Yu Zhang, Tian-Lang Li, Zi-Tong Fu, Quan Qi, Yu-Liang Zhang, Shi-Hui Sun, and Guo-Wei Zhang
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CHINESE medicine ,COVID-19 pandemic ,DATA mining ,MOLECULAR docking ,LUNG injuries - Abstract
Background: As of 2023, coronavirus disease 2019 (COVID-19) is still spreading globally. Therefore, we aim to integrate non-critical COVID-19 high-frequency and high-targeting Chinese medicines to provide a reference for clinical prescriptions to improve COVID-19-related symptoms. Materials and methods: The information on non-critical COVID-19 high-frequency Chinese medicines in the diagnosis and treatment of COVID-19 was obtained by the TCM inheritance support platform. Using network pharmacology and molecular docking technology, high-targeting Chinese medicines with good docking activity with COVID-19 receptors angiotensin-converting enzyme-II (ACE2), 3CLpro and tyrosine-protein kinase receptor UFO (AXL) were obtained. A new prescription for non-critical COVID-19 was established by integrating high-frequency and high-targeting Chinese medicines. Rats with acute lung injury induced by lipopolysaccharide were used as the experimental model. The histopathological changes in the lungs of rats in each group were observed by hematoxylin-eosin staining. The lung coefficient of rats was measured. The levels of IL-6, TNF-a, and IL-1ß in serum were detected by enzyme-linked immunosorbent assay. The mRNA and protein levels of ACE2 and AXL in lung tissue were detected by real-time quantitative polymerase chain reaction and western blot. Results: Through data mining, it was found that there were 39 high-frequency traditional Chinese medicines for non-critical COVID-19 in the diagnosis and treatment guidelines. According to network pharmacology and molecular docking, 30 highly targeted traditional Chinese drugs for COVID-19 were found. The new prescriptions for non-critical COVID-19 were comprehensively obtained, including Glycyrrhizae Radix, Ephedra Herba, Amygdalus Communis Vas, Gypsum Fibrosum, Descurainiae Semen, Atractylodes Lancea, Scutellariae Radix, Amomum Tsao-Ko Crevostet, Forsythiae Fructus, Pogostemon cablin, Magnolia Officinalis. Compared with the LPS-induced lung injury model group, the medium dose of the new prescription group had significantly alleviated pathological changes in lung tissue, decreased lung coefficient, decreased contents of IL-6, TNF-a and IL-1ß, and increased mRNA and protein expression of ACE2 and AXL (P < 0.05). Conclusion: Based on data mining, network pharmacology and molecular docking technology, the new prescription for non-critical COVID-19 established by this method has an anti-inflammatory effect on rats with acute lung injury induced by lipopolysaccharide and can provide a reference for clinicians to alleviate the symptoms related to non-critical COVID-19. [ABSTRACT FROM AUTHOR]
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- 2023
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31. Acinar ATP8b1/LPC pathway promotes macrophage efferocytosis and clearance of inflammation during chronic pancreatitis development
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Guo-wei Zhang, Rong-chang Cao, Wan-jun Yang, Wang Xiao, Xiao-lou Zhang, Hao Xu, Meng Wang, Zhitao Zhou, Huoji Chen, Jia Xu, Xuemei Chen, Jun-ling Zeng, Shu-ji Li, Min Luo, Yan-jiang Han, Xiao-bing Yang, Guo-dong Feng, Yu-heng Lu, Yuan-yuan Ni, Chan-gui Wu, Jun-jie Bai, Zi-qi Yuan, and Jin Jin
- Abstract
Background Noninflammatory clearance of dying cells by professional phagocytes, termed efferocytosis, is fundamental in both homeostasis and inflammatory fibrosis disease but has not been confirmed to occur in chronic pancreatitis (CP). Here, we investigated whether efferocytosis constitutes a novel regulatory target in CP and its mechanisms. Methods PRSS1 transgenic (PRSS1Tg) mice were treated with caerulein to mimic CP development. Sphingomyelin metabolite profiling and epigenetic assays were performed with PRSS1Tg CP models. The potential functions of Atp8b1 in CP model were clarified using Atp8b1-overexpressing adeno-associated virus, immunofluorescence, enzyme-linked immunosorbent assay(ELISA) and lipid metabolomic approaches. ATAC-seq combined with RNA-seq was then used to identify transcription factors binding to the Atp8b1 promoter, and ChIP-qPCR and luciferase assays were used to confirm that the identified transcription factor bound to the Atp8b1 promoter, and to identify the specific binding site. Flow cytometry was performed to analysis proportion of pancreatic macrophages. Results Decreased efferocytosis with aggravated inflammation was identified in CP. The lysophosphatidylcholine (LPC) pathway was the most obviously dysregulated sphingomyelin pathway, and LPC and Atp8b1 expression gradually decreased during CP development. H3K27me3 ChIP-seq showed that increased Atp8b1 promoter methylation led to transcriptional inhibition. Atp8b1 complementation substantially increased the LPC concentration and improved CP outcomes. Bhlha15 was identified as a transcription factor that binds to the Atp8b1 promoter and regulate sphingomyelin metabolism. Conclusion The acinar Atp8b1/LPC pathway acts as an important “find-me” signal for macrophages and plays a protective role in CP, with Atp8b1 transcription promoted by the acinar cell-specific transcription factor Bhlha15. Bhlha15, Atp8b1 and LPC could be clinically translated into valuable therapeutic targets to overcome the limitations of current CP therapies.
- Published
- 2022
32. Retraction notice to 'Inhibition of interleukin-1β plays a protective role in Alzheimer’s disease by promoting microRNA-9-5p and downregulating targeting protein for xenopus kinesin-like protein 2' [Int. Immunopharmacol. 97 (2021) 107578]
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Yan-Yun Zhang, Li-Xia Dong, Hai-Lan Bao, Yu Liu, Feng-Mao An, and Guo-Wei Zhang
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Pharmacology ,Immunology ,Immunology and Allergy - Published
- 2023
33. Concentrations of glucose metabolites in the aqueous humour of diabetic cataract eyes
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Ruoxin Ren, Guo-Wei Zhang, Wen-Yu Yang, Yi Xie, Miao Wei, Huai-Jin Guan, and Min Ji
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Ophthalmology ,General Medicine - Abstract
Purpose Glucose metabolism underpins diabetic cataracts (DCs), but the relationship between the two remains unclear. Here, we tested the aqueous humour (AH) of patients with DCs to elucidate glucose metabolite levels. Methods In this study, aqueous humour (AH) samples were collected preoperatively from DC eyes (n = 37) and age-related cataract eyes (n = 37) from 74 patients (74 eyes) undergoing uncomplicated cataract surgery. The content of glucose, pyruvate, L-lactate were detected by biochemical methods and advanced glycation end-products (AGEs) was detemined using enzyme-linked immunosorbent assay (ELISA) technique. Furthermore, the ratios of glucose/pyruvate and L-lactate/pyruvate in the AH were calculated. In addition, we calculated the correlation between glucose levels and AGEs in the AH. Results The concentrations of glucose, pyruvate and AGEs in the DC group were higher than those in the control group. Significantly lower levels of L-lactate in the AH were found in the DC group. We calculated the glucose/pyruvate ratio and the L-lactate/pyruvate ratio in the AH, which showed that glucose metabolism was changed in the AH from DC patients. Interestingly, we observed that AGEs in the AH were significantly correlated with increased anterior chamber glucose permeability. A stronger correlation was found in the subgroups of male patients, younger patients, and patients with poor glycaemic control status. Conclusions Comparison of the levels of glucose metabolism-related products in the AH in the DC group highlight a potential pathological mechanism for DC from a glucose metabolism perspective. The findings indicated an alteration in the metabolic pathways of energy metabolism and amino acids in the AH of DC patients.
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- 2023
34. Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of Alzheimer’s disease
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Yan-Yun Zhang, Hai-Lan Bao, Yu Liu, Guo-Wei Zhang, Li-Xia Dong, and Feng-Mao An
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0301 basic medicine ,endocrine system ,Cell Survival ,Apoptosis ,Disease ,Biology ,PC12 Cells ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Alzheimer Disease ,Cell Line, Tumor ,microRNA ,Animals ,HMGB1 Protein ,Molecular Biology ,Membrane Potential, Mitochondrial ,Neurons ,Amyloid beta-Peptides ,RNA ,Cell Biology ,Peptide Fragments ,Rats ,Up-Regulation ,Cell biology ,MicroRNAs ,Oxidative Stress ,030104 developmental biology ,030220 oncology & carcinogenesis ,embryonic structures ,RNA, Long Noncoding ,Cellular model ,Research Paper ,Signal Transduction ,Developmental Biology - Abstract
Accumulating data manifest that long non-coding RNA (lncRNAs) are involved in all kinds of neurodegenerative disorders, consisting of the onset and progression of Alzheimer’s disease (AD). The study was for the research of the mechanism of lncRNA H19 (H19) in viability and apoptosis of PC12 cells induced by Aβ(25-35) in a cellular model of AD with the regulation of microRNA (miR)-129 and high mobility group box-1 protein (HMGB1). An AD cellular model of PC12 cells was established using Aβ(25-35). The Aβ(25-35)-induced PC12 cells were transfected with si-H19 or miR-129 mimic to figure their roles in cell viability,apoptosis, mitochondrial membrane potential dysfunction and oxidative stress in AD. Luciferase reporter assay and RNA-pull down assay were employed for verification of the binding relationship between H19 and miR-129 and the targeting relationship between miR-129 and HMGB1. An AD mouse model was induced and brain tissues were collected. H19, miR-129 and HMGB1 were detected in Aβ(25-35)-treated cells and brain tissues of AD mice. Elevated H19, HMGB1 and decreased miR-129 were found in Aβ(25-35)-treated PC12 cells as well as in brain tissues of AD mice. Silenced H19 or elevated miR-129 promoted viability, inhibited apoptosis, prevented mitochondrial membrane potential dysfunction and decreased oxidative stress in Aβ(25-35)-treated PC12 cells. H19 could specifically bind to miR-129. MiR-129 specifically suppressed HMGB1 expression. This study suggests that silenced H19 and up-regulated miR-129 accelerates viability and represses apoptosis of PC12 cells stimulated by Aβ(25-35) in AD, which is beneficial for AD treatment.
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- 2021
35. EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis
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Jie-hui Tan, Jia Xu, Guo-wei Zhang, Yang-chen Jin, Min Luo, Xue-mei Chen, Jun-ling Zeng, Lei Zhou, Shu-ji Li, Rong-chang Cao, Guo-dong Hu, Jin Jin, Jia-yu Lin, Zhao-chang Qi, Huo-ji Chen, and Zhi-tao Zhou
- Subjects
0301 basic medicine ,Proteomics ,Cancer Research ,Molecular biology ,Immunology ,Inflammation ,Apoptosis ,Mice, Transgenic ,Article ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,Pancreatitis, Chronic ,Acinar cell ,Medicine ,Animals ,Humans ,APAF1 ,lcsh:QH573-671 ,Gene knockdown ,business.industry ,lcsh:Cytology ,Membrane Proteins ,Cell Biology ,Middle Aged ,medicine.disease ,Mice, Inbred C57BL ,030104 developmental biology ,Apoptotic Protease-Activating Factor 1 ,Pancreatitis ,030220 oncology & carcinogenesis ,Acute Disease ,Cancer research ,Acute pancreatitis ,medicine.symptom ,business - Abstract
Treatment of acute pancreatitis (AP) and chronic pancreatitis (CP) remains problematic due to a lack of knowledge about disease-specific regulatory targets and mechanisms. The purpose of this study was to screen proteins related to endoplasmic reticulum (ER) stress and apoptosis pathways that may play a role in pancreatitis. Human pancreatic tissues including AP, CP, and healthy volunteers were collected during surgery. Humanized PRSS1 (protease serine 1) transgenic (PRSS1Tg) mice were constructed and treated with caerulein to mimic the development of human AP and CP. Potential regulatory proteins in pancreatitis were identified by proteomic screen using pancreatic tissues of PRSS1Tg AP mice. Adenoviral shRNA-mediated knockdown of identified proteins, followed by functional assays was performed to validate their roles. Functional analyses included transmission electron microscopy for ultrastructural analysis; qRT-PCR, western blotting, co-immunoprecipitation, immunohistochemistry, and immunofluorescence for assessment of gene or protein expression, and TUNEL assays for assessment of acinar cell apoptosis. Humanized PRSS1Tg mice could mimic the development of human pancreatic inflammatory diseases. EMC6 and APAF1 were identified as potential regulatory molecules in AP and CP models by proteomic analysis. Both EMC6 and APAF1 regulated apoptosis and inflammatory injury in pancreatic inflammatory diseases. Moreover, APAF1 was regulated by EMC6, induced apoptosis to injure acinar cells and promoted inflammation. In the progression of pancreatitis, EMC6 was activated and then upregulated APAF1 to induce acinar cell apoptosis and inflammatory injury. These findings suggest that EMC6 may be a new therapeutic target for the treatment of pancreatic inflammatory diseases.
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- 2020
36. A case of double primary carcinoma was accidentally found during operation: hepatocellular carcinoma and appendiceal mucinous adenocarcinomas
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Hai-Liang Li, Yi Zhou, Peng Gao, Xian-Cheng Zeng, Guo-Wei Zhang, and Bo-Hao He
- Subjects
digestive system diseases - Abstract
Background: Synchronous double primary cancer consisting of hepatocellular carcinoma (HCC) and appendiceal mucinous adenocarcinomas has not been reported in the world so far. Liver metastasis of digestive tract tumor is so common that it is easy to ignore the existence of double primary cancer in the digestive system. Appendiceal malignant tumor itself is easy to be misdiagnosed or missed. For the better understanding of HCC and appendiceal mucinous adenocarcinomas, the information of our recent case has been summarized.Case presentation: A 58-year-old man was admitted with repeated upper abdominal pain for one week. Imaging examination and liver biopsy pathology were diagnosed as hepatocellular carcinoma. Colonoscopy showed no obvious organic lesions in the terminal ileum and the whole large intestine. We had anatomical resection of S5, S6 segment of liver. At the same time, two suspicious lesions in the right liver were ablated by microwave. But at the end of the operation, we accidentally found a hard mass about 3cm x 3cm in size at the end of the appendix. And the rapid pathological results showed appendiceal adenocarcinoma, so we had a right hemicolectomy. So far, the diagnosis of the patient was changed to synchronous double primary cancer consisting of HCC and appendiceal mucinous adenocarcinomas.Conclusions: It is the first time we report a case of synchronous double primary hepatic cancer consisting of HCC and appendiceal mucinous adenocarcinomas in the world. Due to the rarity and unspecific clinical features, it is extremely challenging to be diagnosed preoperatively. It requires us to have a more comprehensive understanding of the patient's medical history, careful physical examination and comprehensive exploration of the abdominal cavity during the operation, so as to avoid missed diagnosis. Patients with HCC and appendiceal mucinous adenocarcinomas are generally associated with poor prognosis. It needs we put forward new countermeasures for the new question.
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- 2022
37. Properties and characteristics of the Srivastava-Khairnar-More integral operator.
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Zhi-Gang Wang, Guo-Wei Zhang, and Feng-Hua Wen
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- 2012
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38. Viral hepatitis B and C infections increase the risks of intrahepatic and extrahepatic cholangiocarcinoma: Evidence from a systematic review and meta-analysis
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Wan-Yan Zhou, Lei Zhou, Guo-wei Zhang, Rong-chang Cao, and Jie-hui Tan
- Subjects
Adult ,Male ,Hepatitis B virus ,medicine.medical_specialty ,Hepatitis C virus ,Hepacivirus ,medicine.disease_cause ,Gastroenterology ,Bile duct cancer ,Cholangiocarcinoma ,Extrahepatic Cholangiocarcinoma ,03 medical and health sciences ,0302 clinical medicine ,Bile Ducts, Extrahepatic ,Risk Factors ,Internal medicine ,parasitic diseases ,medicine ,Humans ,Antigens, Tumor-Associated, Carbohydrate ,Lymph node ,Intrahepatic Cholangiocarcinoma ,Aged ,business.industry ,fungi ,Middle Aged ,Hepatitis B ,medicine.disease ,Hepatitis C ,digestive system diseases ,Bile Ducts, Intrahepatic ,medicine.anatomical_structure ,Bile Duct Neoplasms ,030220 oncology & carcinogenesis ,Meta-analysis ,cardiovascular system ,Female ,Original Article ,030211 gastroenterology & hepatology ,Lymph Nodes ,alpha-Fetoproteins ,Lymph ,business - Abstract
Background/aims Previous study has shown a positive relationship between the hepatitis B virus (HBV) or hepatitis C virus (HCV) infection and cholangiocarcinoma (CCA); however, their correlation with different anatomical sites of CCA (i.e. ICC and ECC) has not been revealed. This study aims to evaluate the association of HBV or HCV infection with CCA, including the intrahepatic cholangiocarcinoma (ICC) and extrahepatic cholangiocarcinoma (ECC), and to determine the roles of α-1 fetoprotein (AFP), CA19-9, and lymph node involvement in CCA with HBV infection. Materials and methods Relevant studies published between 2004 and 2016 were systematically searched and retrieved from PubMed, SpringerLink, and Science Direct using key terms such as "cholangiocarcinoma", "bile duct cancer", "extrahepatic cholangiocarcinoma", and "intrahepatic cholangiocarcinoma". The demographic, clinical, and laboratory data were extracted from the included studies, and the meta-analysis was performed using RevMan and STATA 11.0 software. Results A total of 13 studies with CCA matched the inclusion criteria in this meta-analysis, including 7,113 CCA patients and 24,763 controls. This meta-analysis showed that the HBV or HCV infections can significantly increase the risk of CCA, including ICC and ECC. In addition, the higher levels of AFP, lower levels of CA19-9, and lymph node involvement were detected in the CCA patients with HBV infection as compared to those without. Conclusion The HBV and HCV infections significantly increased the risk of CCA, as well as ICC and ECC. The involvement of AFP, CA19-9, and lymph nodes may play an important role in the diagnosis of CCA.
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- 2020
39. P53 Activated by ER Stress Aggravates Caerulein-Induced Acute Pancreatitis Progression by Inducing Acinar Cell Apoptosis
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Guo-wei Zhang, Xue-mei Chen, Lei Zhou, Shi-jing Ren, Jie-hui Tan, Jia Xu, Wan-Yan Zhou, and Zhen-yu Lin
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Genetically modified mouse ,Physiology ,Apoptosis ,Mice, Transgenic ,Acinar Cells ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Acinar cell ,Animals ,Humans ,Trypsin ,Activator (genetics) ,Chemistry ,Endoplasmic reticulum ,Gastroenterology ,Endoplasmic Reticulum Stress ,Blot ,Pancreatitis ,030220 oncology & carcinogenesis ,Unfolded protein response ,Cancer research ,Immunohistochemistry ,030211 gastroenterology & hepatology ,Tumor Suppressor Protein p53 ,Ceruletide - Abstract
Acute pancreatitis (AP) is a severe pancreatic disorder that remains associated with high mortality due to a lack of effective drugs and management strategies. This study aimed to investigate the molecular pathogenic mechanisms of AP involving p53 and endoplasmic reticulum (ER) stress pathways. Expression of PRSS1 and p53 in human AP tissues was detected by immunohistochemistry and Western blotting. AP was induced with caerulein in humanized PRSS1 transgenic mice, and its severity was verified by histological imaging, evaluation of edema, serum amylase, and trypsin activity assays. A transferase-mediated d-UTP nick end-labeling assay was performed to evaluate acinar cell apoptosis associated with AP. The expression of ER stress genes was assessed by quantitative RT-PCR (qRT-PCR) and Western blotting. PRSS1 and p53 were highly expressed in human AP tissues. Expression of human PRSS1 in caerulein-treated mice induced significant acinar cell apoptosis and AP progression. P53 knockout significantly suppressed AP progression in humanized PRSS1 transgenic mice. The ER stress pathway was activated by PRSS1 and mediated the progression of AP in mouse pancreatic tissues. Application of a p53 inhibitor effectively ameliorated caerulein-induced AP in PRSS1 transgenic mice, while a p53 activator promoted the progression of AP. P53, which was activated by the ER stress pathway, promoted the progression of AP in mice expressing PRSS1 by inducing acinar cell apoptosis.
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- 2020
40. Penetrance of
- Author
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Juan-Juan, Xie, Guo-Wei, Zhang, Hai-Yue, Cui, Na, Li, Xing-Xing, Liu, Meng-Yao, Hao, Shao-Wen, Wang, and Hong, Lu
- Subjects
Cytoskeletal Proteins ,DNA Mutational Analysis ,Mutation ,Humans ,Penetrance ,Eye Proteins ,Glaucoma, Open-Angle ,Glycoproteins - Abstract
To investigate the penetrance ofWe searched all studies that reported the penetrance ofFifty-two studies were included in this analysis after screening. Meta-analysis of the penetrance ofThe penetrance of
- Published
- 2022
41. Highly bioactive iridium metal-complex alleviates spinal cord injury via ROS scavenging and inflammation reduction
- Author
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Zhi-Sheng Ji, Gui-Bin Gao, Yan-Ming Ma, Jian-Xian Luo, Guo-Wei Zhang, Hua Yang, Nan Li, Qing-Yu He, and Hong-Sheng Lin
- Subjects
Inflammation ,Biophysics ,Bioengineering ,Iridium ,Antioxidants ,Biomaterials ,Mice ,Superoxide Dismutase-1 ,Spinal Cord ,Mechanics of Materials ,Coordination Complexes ,Ceramics and Composites ,Animals ,Gliosis ,Reactive Oxygen Species ,Spinal Cord Injuries - Abstract
Generation of a promising antioxidative reagent with superior biocompatibility is urgently needed to remedy spinal cord injuries (SCI), repair the damaged neurons and restrain the secondary injuries caused by inflammation-induced oxidative stress. Inhibitory elements in the injury sites and necessitous inherent neural regeneration ability were major challenges for functional recovery after spinal cord injuries. We here developed a highly bioactive iridium complex (IrFPHtz) with enhanced antioxidative activities and improved SCI therapeutic efficacy. Both in vivo and in vitro, IrFPHtz has exhibited neuroprotective and anti-inflammatory properties. Mechanically, IrFPHtz directly targets SOD1 and upregulates the expression of SOD1 to eliminate the excess Reactive Oxygen Species (ROS) production induced by SCI, and thus protecting neuron cells from further damage. As a result, IrFPHtz safeguarded the neurons and myelin sheaths against trauma, lessened glial scar conformations and facilitated the repair of neurons and long axon expansion in the glial scar. Furthermore, IrFPHtz significantly ameliorated the behavioral functions of SCI mice and promoted a satisfactory curative effect. Therefore, this study sheds light on a novel method for SCI treatment using IrFPHtz as a potential drug and implicates the clinical significance of metal complexes in diseases featuring with upregulated ROS species.
- Published
- 2022
42. A Cinar ATP8b1/LPC Pathway Promotes Macrophage Efferocytosis And Clearance of Inflammation During Chronic Pancreatitis Development
- Author
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Rong-chang Cao, Wan jun Yang, Wang Xiao, Xiao-lou Zhang, Hao Xu, Meng Wang, Zhi-tao Zhou, Huo-ji Chen, Jia Xu, Xue-mei Chen, Jun-ling Zeng, Shu-ji Li, Min Luo, Yan-jiang Han, Xiao-bing Yang, Guo-dong Feng, Yi-heng Lu, Yuan-yuan Ni, Chan-gui Wu, Jun-jie Bai, Zi-qi Yuan, Jin Jin, and Guo-wei Zhang
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
43. The mechanism of TCM prevention and treatment of heavy COVID-19 based on data mining and network pharmacology
- Author
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Zi-Tong Fu, Jia-Yu Wang, Yi Liu, Jia-Ming Xiong, and Guo-Wei Zhang
- Published
- 2023
44. Comment on'mechanisms of compound Kushen injection for treatment of bladder cancer based on bioinformatics and network pharmacology with experimental validation'
- Author
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Xiang-Ying Tian and Guo-Wei Zhang
- Published
- 2023
45. Young Chinese children without seroprotective hepatitis B surface antibody could be at risk of hepatitis B virus infection through horizontal transmission
- Author
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Hong Zhu, Wu‐Ning Mo, Guo‐Wei Zhang, Wen‐Li Zhang, Sheng‐Tao Li, Nan Wu, Xin Lv, Yun‐Feng Liu, Xiaowang Qu, Ping Si, Wen‐Yuan Shi, Rui Zhao, Li‐Yan Ge, Zhen‐Wen Zhou, Ping Liu, Jun‐Yi Liao, Hong‐Zhi Wang, Xiao-Qun Zheng, Hong‐Juan Li, Wenpei Liu, Zhi‐Li Yang, Yu‐Qiang Zheng, Zheng Yang, Tian Wu, Yu‐Jing Song, Jian Yu, and Xiao-Ben Pan
- Subjects
Hepatitis B virus ,China ,Hepatitis B Surface Antigens ,Hepatology ,business.industry ,Infant ,Hepatitis B ,medicine.disease_cause ,Virology ,law.invention ,Infectious Diseases ,Transmission (mechanics) ,law ,Hepatitis B surface antibody ,medicine ,Humans ,Hepatitis B Antibodies ,Child ,business ,Horizontal transmission - Published
- 2019
46. Surgical Techniques for the Laparoscopic Treatment of Bile Duct Stones in Patients With a History of Upper Abdominal Operations: Retrospective Cohort Study
- Author
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Guo-wei Zhang, Lei Zhou, and Jie-hui Tan
- Subjects
Male ,Reoperation ,medicine.medical_specialty ,Gallstones ,Group B ,03 medical and health sciences ,0302 clinical medicine ,Blood loss ,Abdomen ,medicine ,Humans ,In patient ,Laparoscopy ,Retrospective Studies ,Cholangiopancreatography, Endoscopic Retrograde ,Common Bile Duct ,Laparotomy ,medicine.diagnostic_test ,Bile duct ,business.industry ,Retrospective cohort study ,Middle Aged ,Surgery ,Treatment Outcome ,medicine.anatomical_structure ,Cholecystectomy, Laparoscopic ,030220 oncology & carcinogenesis ,Feasibility Studies ,Female ,030211 gastroenterology & hepatology ,Abdominal operations ,business ,Laparoscopic treatment ,Follow-Up Studies - Abstract
PURPOSE Few authors have studied applying the laparoscopic approach in patients with previous upper abdominal operations, but no comparison has been made between laparoscopic and open approaches in patients with previous upper abdominal operations. This article aims to introduce surgical techniques and details in treatment to surgeons specialized in minimally invasive surgery. MATERIALS AND METHODS From January 2010 to January 2018, 460 eligible patients were divided into 3 groups and analyzed retrospectively. Group A: patients with a history of upper abdominal operations who underwent laparoscopy (n=124); group B: patients without a history of upper abdominal operations who underwent laparoscopy (n=140); and group C: patients with a history of upper abdominal operations who underwent an open operation (n=196). Group A was the experimental group; groups B and C served as the control groups. RESULTS No significant difference was found between groups A and B. Significant differences were found between groups A and C in estimated blood loss (258.3±67.2 vs. 424.7±103.7 mL, P
- Published
- 2019
47. Designing and Practical Computing of Fire Isolation Belt in Large Space Building
- Author
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Guo-qing, ZHU, Guo-wei, ZHANG, Xiao-lan, WANG, and Wei-hua, WU
- Published
- 2011
- Full Text
- View/download PDF
48. The Comparison of Fire Numerical Simulations based on the Large Space Factory Fire Experiment
- Author
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Guo-wei, Zhang, Guo-qing, Zhu, Xiao-lan, Wang, and Wei-hua, Wu
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- 2011
- Full Text
- View/download PDF
49. The Comparative Analysis of Numerical Simulation and Experimental Studies in Large Space Building
- Author
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Wei-hua, Wu, Guo-qing, Zhu, Xiao-lan, Wang, and Guo-wei, Zhang
- Published
- 2011
- Full Text
- View/download PDF
50. The Engineering Application and Case Analysis of ‘Quasi-security zone’ in Large Space Building
- Author
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Xiao-lan, WANG, Guo-qing, ZHU, Guo-wei, ZHANG, and Wei-hua, WU
- Published
- 2011
- Full Text
- View/download PDF
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