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Metamitron (MTM) is a typical and widely used triazine herbicide in agricultural production. Its moderate toxicity and high residue in the environment have deleterious impacts on human health. The establishment of a rapid and efficient MTM detection method is of great significance. In this study, a magnetic-bead SELEX (systematic evolution of ligands by exponential enrichment) system was developed to select the MTM aptamers with high affinity and specificity. Through 10 rounds of screening, six candidate aptamers with the highest abundance were obtained by high-throughput sequencing. The homology, secondary structure, and affinity analyses were performed. The aptamer named MTM-6 was selected as the optimal aptamer with the dissociation constant (

The search for alternatives to chemicals from natural products as precursors for the preparation of highly doped carbon dots (CDs) remains challenging. Novel CDs (W-CDs) were synthesised using a one-step pyrolysis method with wastewater hyacinth as the sole carbon and nitrogen source at a mild temperature without using any surface-activating reagents or salt. The obtained W-CDs emitted strong blue fluorescence under 365 nm UV light excitation, with a quantum yield of 15.12%. The Box-Behnken design of the response surface methodology was applied to optimize the W-CD preparation conditions, including the reaction temperature, reaction time and weight of water hyacinths. The temperature was found to be the most important factor affecting the fluorescence intensity of the W-CDs. Additionally, the fluorescence sensor based on W-CDs demonstrated excellent selectivity towards ferric (Fe) ions, with a limit of detection of 2.35 μM. The fluorescent sensor was successfully applied for detecting Fe

This paper illustrates the establishment of a colourimetric method for detection of the fungicide tebuconazole (TEB) in aqueous samples based on an unmodified TEB-specific aptamer and gold nanoparticles (AuNPs). In the absence of TEB, the AuNPs are coated with a TEB-specific aptamer and then stably dispersed a NaCl solution of high concentration, leading to a red solution and producing a maximum UV absorption peak at 520 nm. In the presence of TEB, due to the specific high affinity between TEB and the TEB-specific aptamer, the aptamer combines with TEB to form stable compounds, causing the AuNPs to be exposed in the solution and aggregate. The aggregated AuNPs turn the solution from red to blue, presenting a maximum UV absorption peak at 650 nm. Therefore, the concentration of TEB in the system can be quantitatively detected through the changes in absorbance. This TEB selective colourimetric biosensor detects TEB over a linear concentration range of 20 to 400 nM (R = 0.99385) and has a limit of detection (LOD) of 4.13 nM. The average recovery of TEB is 94.9–104.8 % in the application of actual water samples with the relative standard deviations (RSD) ranging from 1.01 to 5.34 %. With considerable sensitivity and selectivity, this aptasensor indicates great potential for TEB detection in aqueous samples.

Ubiquitination and deubiquitination of target proteins is an important mechanism for cells to rapidly respond to changes in the external environment. The deubiquitinase, cylindromatosis (CYLD), is a tumor suppressor protein. CYLD from Drosophila melanogaster participates in the antimicrobial immune response. In vertebrates, CYLD also regulates bacterial-induced apoptosis. However, whether CYLD can regulate the bacterial-induced innate immune response in crustaceans is unknown. In the present study, we reported the identification and cloning of CYLD in Chinese mitten crab, Eriocheir sinensis. Quantitative real-time reverse transcription polymerase chain reaction analysis showed that EsCYLD was widely expressed in all the examined tissues and was upregulated in the hemolymph after Vibrio parahaemolyticus challenge. Knockdown of EsCYLD in hemocytes promoted the cytoplasm-to-nucleus translocation of transcription factor Relish under V. parahaemolyticus stimulation and increased the expression of corresponding antimicrobial peptides. In vivo, silencing of EsCYLD promoted the removal of bacteria from the crabs and enhanced their survival. In addition, interfering with EsCYLD expression inhibited apoptosis of crab hemocytes caused by V. parahaemolyticus stimulation. In summary, our findings revealed that EsCYLD negatively regulates the nuclear translocation of Relish to affect the expression of corresponding antimicrobial peptides and regulates the apoptosis of crab hemocytes, thus indirectly participating in the innate immunity of E. sinensis.

Integrins are cellular adhesion molecules that mediate cell-cell, cell-extracellular matrix, and cell-pathogen interactions. Integrins can stimulate various signaling pathways by binding to different ligands, thereby exerting immunological functions. While integrins have been found to primarily play a role in bacterial agglutination, phagocytosis, and inhibition of apoptosis in invertebrates, the specific signaling pathway and mechanism of action remain unclear. In vertebrates, β1 integrin and extracellular matrix interactions can associate with focal adhesion kinase (FAK) to initiate MAPK/ERK signaling and regulate cell survival; however, in invertebrates (e.g., Chinese mitten crab), the mechanisms of integrins are poorly understood. The purpose of this study was to investigate whether integrinβ1/FAK activation of the MAPK/ERK pathway regulates hemocyte survival and the associated mechanism. Treatment with an integrinβ1 inhibitor RGD (a conserved tripeptide Arg-Gly-Asp), decreased the levels of FAK and ERK expression and phosphorylation, followed by an intensification of apoptosis. Similar results were obtained following siRNA knockdown of integrinβ1 expression. We further found that the attenuation of ERK phosphorylation enhanced the level of Caspase-3 expression. Together, these findings suggest that integrinβ1 activates the FAK/ERK signaling cascade and is involved in the survival of Chinese mitten crab hemocytes.

The presence of pesticide residues has become one of the main risk factors affecting the safety and quality of agro-food. In this study, a multi-residue method for the analysis of 284 pesticides in five local fruit cultivars in Shanghai was developed based on ultrahigh-performance liquid chromatography-quadrupole time-of-fight mass spectrometry (UPLC-QTOF/MS). The limits of determination and the limits of quantitation of pesticides were 0.6–10 and 2–30 µg/kg, respectively. A total of 44, 10, 10, 18, and 7 pesticides were detected in strawberries, watermelons, melons, peaches, and grapes, respectively. The pesticide levels in 95.0% of the samples were below the maximum residual limits (MRLs) prescribed by China, and in 66.2% of the samples below the EU MRLs. The dietary risk assessment study showed big differences in the chronic and acute exposure risk values among different Chinese consumer groups. Through fruit consumption, children/females showed higher exposure risks than adults/males. But both the risk values were less than 100%, indicating that potential dietary risk induced by the pesticides was not significant for Chinese consumers. Nevertheless, certain measures are needed for both growers and government in order to decrease the MRL-exceeding rate of pesticide residues and ensure the quality and safety of fruits for consumers.

Soil fumigation using 1,3-dichloropropene (1,3-D) and chloropicrin (CP) is an important strategy for agriculture production; however, excessive emissions can cause air pollution and possible human exposure. In this study, solar light-driven CuInS2/ZnS:Al-TiO2 photocatalytic film was prepared through spin-coating on the flexible polyethylene terephthalate (PET) substrate of 0.1 mm. Using the photocatalytic film, degradation of 1,3-D was inhibited in the Pci-clor 60 formulation of 1,3-D and CP. However, the degradation of CP was accelerated in this formulation, and the half-life was shortened from 0.66 to 0.40 h. Emissions of 1,3-D from soil to the air were reduced by 97.30%, 97.17%, 47.10%, and 7.88%, for treatments of D + Film, D + C + Film, D + PET, and D, respectively. The efficiencies for reducing 1,3-D emission were significantly improved by about 1.1 and 11.3 times using the film, compared with using the PET alone and no film, respectively. Furthermore, fumigation effects on nematodes could still achieve higher than 90%. The findings provided a basis for the practical application of quantum dot films to reduce soil fumigants emissions by photocatalytic degradation.

In order to study a new clean and efficient combustion mode, which can relieve the pressure of traditional energy and ensure low emissions, in this study, a diesel/natural gas dual fuel engine is designed by non-dominant sorting genetic algorithm (NSGA-?), and its thermodynamic characteristics are studied. The WP10.290 Diesel engine is modified into a diesel/natural gas dual fuel engine. The emissions of harmful substances and thermal efficiency of the modified engine under different working conditions are compared. The combustion chamber structure and adaptability between combustion chamber and injection parameters are optimized by using NSGA-II algorithm and CFD software. The results show that the emission of NOx and CH4 and the fuel consumption rate can be reduced simultaneously by using the composite combustion model compared with the original engine. When the CH4 emission is close to zero, the fuel consumption rate decreases obviously, and NOx slightly increases. When the angle between the injection holes is 141.57? the amount of NOx in the cylinder is large. When the injection advance angle is 21.91?CA, the pressure in the cylinder is the highest, the CH4 production is the lowest, the NOx production is higher, and the oxygen content in the combustion mixture is less. The NOx production is the lowest. diesel/natural gas dual fuel engine can ensure efficient combustion while reducing emissions. In this study, the performance of the dual fuel engine at various speeds can be further studied, which can provide theoretical support for the design of diesel/natural gas dual fuel engine.

Netilmicin (NET) is an antibiotic widely used in healthcare and agriculture, but it can accumulate in the environment to threat human health. Netilmicin (NET) is an antibiotic used for veterinary purposes, for human therapy and for agricultural purposes. Therefore, there is a need to develop high-sensitive measuring methods to detect NET. Aptamer-based detecting methods are highly sensitive, inexpensive, and portable. In this study, we developed an aptamer-based fluorescence method to detect and quantify NET. NET was first conjugated to magnetic beads by amidation reaction and then NET-coated beads were used as the stationary phase to isolate aptamers by systematic evolution of ligands by exponential enrichment (SELEX) screening method. After ten rounds of SELEX screening, 32 aptamers with NET-binding affinity were obtained and the candidate aptamer APT-21 was finally chosen by comprehensively comparing their secondary structure characters and NET-binding affinity. APT-21 bound to NET with high affinity (Kd = 194.1 nmol/L) and high specificity that it displayed low cross-binding activities on 7 different structural analogs. We also developed a fluorometric assay using SYBR Green I (SG-I) and the APT-21. Key experimental parameters were optimized, including buffer system, SG-I and APT-21 reaction time, SG-I concentration, and aptamer concentration, to improve the detecting sensitivity. Our results suggest that the low limit of detection (LOD) of this method reached a low level of 1.95 nM and it also exhibited a good linear range up to 200 nM. Moreover, we successfully applied our method to detect the NET spiked in tap water and river water with good recoveries in the range from 97% to 111%. In conclusion, our current study isolated a NET-specific aptamer and developed an aptamer-based quantification method, which is promising to apply to detect NET in environmental samples.

A Flash Lidar utilizing a novel Super-Resolution technique has been developed for providing Terrain Relative Navigation and Hazard Avoidance capabilities onboard landing vehicles. Performance of this lidar for space and aerial vehicles will be discussed.

As the most typical example of mRNA variable splicing, Down Syndrome Cell Adhesion Molecule (Dscam) can produce a large number of mRNA isomers. It plays an important role not only in the nervous system, but also in the immune system. In Eriocheir sinensis, the extracellular region of Dscam has three variable domains, which can produce 25, 34 and 18 exons and encode the N-terminal region of immunoglobulin (Ig) 2 and Ig3 domains, and the entire Ig7 domain, respectively. In addition to three variable domains, the extracellular non-variable region of Dscam also includes many Ig domains and fibronectin type III (FNIII) domains. However, the role of the extracellular non-variable region function of Dscam in the immune defense of E. sinensis is unclear. In this study, we focused on the role of the extracellular non-variable region of Dscam in crab immune defense. The results indicate that the extracellular non-variable region of Dscam can bind bacteria and has bacteriostatic function. At the same time, the extracellular non-variable region of Dscam can also directly promote bacterial clearance by promoting phagocytosis of hemocytes.

Ceramic membrane condensers that are used for water and waste heat recovery from flue gas have the dual effects of saving water resources and improving energy efficiency. However, most ceramic membrane condensers use water as the cooling medium, which can obtain a higher water recovery flux, but the waste heat temperature is lower, which is difficult to use. This paper proposes to use the secondary boiler air as the cooling medium, build a ceramic membrane condenser with negative pressure air to recover water and waste heat from the flue gas, and analyze the transfer characteristics of flue gas water and waste heat in the membrane condenser. Based on the experimental results, it is technically feasible for the ceramic membrane condenser to use negative pressure air as the cooling medium. The flue gas temperature has the most obvious influence on the water and heat transfer characteristics. The waste heat recovery is dominated by latent heat of water vapor, accounting for 80% or above. The negative pressure air outlet temperature of the ceramic membrane condenser can reach 50.5 °C, and it is in a supersaturated state. The research content of this article provides a new idea for the water and waste heat recovery from flue gas.

Trichlorphon, a common organophosphorus pesticide (OPs), is widely used in aquaculture to prevent aquatic insects from infecting cultured objects as well as to control the excessive proliferation of plankton in water bodies. However, its repeated use time can contaminate water bodies and impart direct/indirect toxicity to beneficial aquatic species. However, the underlying mechanism regarding toxicity and cellular metabolism remains unclear. Understanding the mechanism would enable the standardized use and management of OPs and their use in the aquatic environment. Here, low concentration of trichlorphon (5 × 10−5 g/L) was used to construct a hepatopancreatic transcriptional library 30 d, 60 d and 90 d after exposure using RNA-Seq. We detected 649, 148, and 2949 DEGs in the hepatopancreas of E. sinensis for the Tri01 vs. Ctr01, Tri02 vs. Ctr02 and Tri03 vs. Ctr03 library, respectively. The results of KEGG pathway enrichment analysis showed that DEGs were mainly enriched in signal transduction, carbohydrate metabolism, transport and catabolism, endocrine system, and digestive system. Also, under trichlorfon stress, DEGs of E. sinensis were enriched in thyroid hormone signaling pathways, protein digestion and absorption, cancer pathways, etc. The significant DEGs were mainly related to metabolism and the apoptosis and autophagy pathways. This study lays a foundation for further revealing the effects of long-term trichlorfon stress on E. sinensis as well as the potential physiological toxicity. The relevant transcriptome data could provide a reference for the molecular toxicological evaluation of trichlorfon in aquaculture.

Granulation of biochar-based fertilizer is one potential method to reduce transportation costs, provide for enhanced handling, and decrease the loss of fertilizer during soil application. This study aimed to synthesize binder-free biogas residue biochar-based fertilizer (RBF) pellets and investigate their physical properties and slow-release potential. Results showed that the physical properties and forming quality of the pellets reached the best when the moisture content was 7.84%, the diameter was 7 mm, the compression speed was 49.54 mm/min, and the molding pressure was 7.5 MPa. Sustained-release kinetic analysis and characterization results identified that the RBF had excellent nitrogen (N), phosphorus (P), and potassium (K) sustained release properties. The sustained release of nutrients gradually increased with the drying temperature, and the sustained-release effect of P was the best, followed by that of N and K. Therefore, RBF pellets may be applied as a green slow-release fertilizer in agricultural production. Physical, chemical, and slow-release properties could be improved by optimizing the drying and granulation process parameters, thus providing a new idea for the combination of kitchen waste recycling and sustainable agricultural development.

In this study, a numerical and experimental investigation was carried out to research the effect of three types of working fluids (i.e., helium, nitrogen and argon) on the performance of a thermoacoustic Stirling engine. To predict and analyze thermoacoustic conversion capacity of the engine, a wide range of critical parameters (e.g. onset temperature, working frequency and pressure oscillations) were selected as analysis parameters. According to the results, a relatively optimum value of mean pressure (0.7–1.0 MPa) appears when the thermoacoustic engine is filled with helium. Compared to other working fluids (i.e, nitrogen and argon), the engine could work (onset temperature below 500 °C) in a relatively high mean pressure range (0.4–2.6 MPa) when using helium. Furthermore, working frequency of helium was found to be nearly three times that of the other two gases regardless of operating conditions. Moreover, when using nitrogen and argon as working gases, the pressure amplitude of thermoacoustic engine was significant under the condition of the same mean pressure. The findings in this study are helpful to improve the practicability of thermoacoustic engine.

Heat transfer in nanofluids due to natural convection and pool boiling (including subcooled boiling and saturated boiling) is analyzed by hot wire method based on the effects of ultrasonic waves in Al2O3 nanofluids. The heat transfer characteristics due to ultrasonic wave propagation in Al2O3 nanofluids under different flow regimes were studied through experiments. In the natural convection state, ultrasonic waves enhance heat transfer in Al2O3 nanofluids, with the highest strengthening heat transfer efficiency reaching 128%. However, the increased heat transfer efficiency showed a downward trend with increased heat flux. Heat transfer in the nanofluid is enhanced by ultrasound when the main body temperature of the fluid is 50 °C, 70 °C and 80 °C under subcooled boiling conditions. However, when the main body temperature is 60 °C, heat transfer in the nanofluid is inhibited by ultrasound, i.e., under saturated boiling condition, ultrasound will still enhance heat transfer in the nanofluids, and the enhanced heat transfer efficiency will decrease with increased heat flux. This paper analyzes experimental results from the aspects of nanoparticle deposition on the heat transfer surface under the influence of ultrasound, the distribution of nanoparticles in liquid, and forces between nanoparticles. In addition, the boiling curves and critical heat flux (CHF) in Al2O3 nanofluids were obtained with and without ultrasound.

Micro-porous ceramic membrane tube is a new type of condensation material which can synchronously recover both water vapor and its accompanied latent heat from exhausted flue gas. The recovery outcome is helpful to reduce amount of make-up water and enhance boiler efficiency of thermal power plant. In this study, an experiment has been carried out to investigate condensation heat-transfer, water-recovery, and flow-resistance performance of micro-porous ceramic membrane tube bundle (CMTB), respectively. In addition, real exhausted flue gas with inlet flow rate ranging from 390 to 750 m3/h, temperature ranging from 323 K to 373 K was used as experimental feed gas. Results showed that the condensation heat-transfer process got more apparently with increasing of flue gas inlet temperature (from 335.3 K to 364.8 K). In addition, both cooling water flow rate and heat-transfer temperature difference had a remarkable influence on the water-recovery performance of the CMTB, and maximal water-recovery mass flow rate could reach 0.0336 × 103 kg/h. Most of all, velocity drop of flue gas was controlled within an appropriate range (from 28.94 m3/h to 178.78 m3/h). The application analysis on the CMTB gives a good perspective for designing high efficiency membrane-type condenser to recover water vapor from exhausted flue gas and make good use of its latent heat in the thermodynamic system of power plant.

In a coal-fired power plant, locating the leak area during boiler hot-state operation has practical engineering value, has apart from its significance in proper shutdown procedures, repair-time reduction, and reduction of financial losses. In this study, we adopted a three-dimensional space location algorithm based on acoustic array to determine the location of water-cooling wall tube leaks in real time. We used a spherical interpolation algorithm to solve the location problem and adopted a time delay estimation based on SWITCH function to obtain the time of arrival. An acoustic location test platform was constructed for studying location accuracy in different location condition. Finally, a field study was performed to verify the spherical interpolation algorithm’s feasibility and practicability in a boiler. Results show that the proposed three-dimensional, eight-element array can locate the leak area during boiler hot-state operation.

The morphology, mineralogy, and sintering characteristics of different lignite ashes prepared at varying temperatures in various lab-scale furnaces were investigated concerning the effect of K2CO3 addition. Lignites with and without K2CO3 addition were oxidised in a plasma asher at 423 K, a muffle furnace at 1073 K and a drop tube furnace (DTF) at 1773 K. Sintering temperatures of the ashes were determined, and their mineralogical and morphological features were analysed using XRD and SEM-EDS. Results showed that ash sintering temperature was increased as combustion temperature increased but decreased as K2CO3 addition ratio increased. Moreover, sintering temperatures of ashes rich in alumina oxide were the highest among the lignite ashes prepared at the same temperature. This trend remains valid for ashes of K2CO3-doped lignites. SEM-EDS results revealed that ashes from plasma asher were irregular and loose whereas the DTF ashes of the K2CO3-doped lignites were mostly spherical in shape, having incurred severe sintering. The lignite ashes from plasma asher tended to be enriched in boehmite and arcanite. At 1723 K as in DTF, new mineral phases including mullite and kalsilite were detected. When K2CO3 was added into lignites, kalsilite, microcline, and potassium sulphate of low melting-points were identified, consequently decreased ash sintering temperature.

Furnace exit gas temperature (FEGT) is an important physical parameter of energy conservation and environmental protection in a coal-fired boiler furnace; however, to date, there is no effective method for monitoring it continuously online. The recently developed acoustic pyrometry is a comparatively advanced method of temperature measurement which possesses the essential characteristics of traditional temperature measurement approaches. We experimentally implemented online FEGT monitoring using acoustic pyrometry, and we could successfully replace the flue gas temperature probes successfully. Two key problems of the acoustic pyrometry technique, i.e. the acoustic signal and time delay estimation, were solved. We adopted the linear sweeping frequency signal as a sound source signal; its sweeping frequency ranged from 500 to 4000 Hz and the sweeping cycle was 0.1 s. We used a generalized cross-correlation technique with PHAT (Phase Transformation) and ML (Maximum Likelihood) as the time delay estimation method when the boiler was in different states. The actual operation of the monitor indicated that the acoustic pyrometry system was accurate and reliable.

Growth of metal oxide layers on quantum dots (QDs) has been regarded as a good way to improve the photostability of QDs. However, direct growth of metal oxides on individual QD remains a great challenge. Here we report a novel approach to rapidly anchor metal oxides on QD surfaces through a sacrificial oxidation of a self-metal source strategy. As typical core/shell QDs, CdSe/CdS or aluminum doped CdSe/CdS (CdSe/CdS:Al) QDs were chosen and treated with peroxide (benzoyl peroxide). Self-metal sources (cadmium or/and aluminum) can be easily sacrificially oxidized, leading to the quick growth of cadmium oxide (CdO) or aluminum/cadmium hybrid oxides (Al2O3/CdO) on the surface of individual QD for improved photostability. Compared with CdO, Al2O3 possesses excellent barrier properties against moisture and oxygen. Therefore, CdSe/CdS QDs with the protection of an Al2O3/CdO hybrid layer show much superior photostability. Under strong illumination with blue light, the QDs coated with the Al2O3/CdO hybrid layer retained 100% of the original photoluminescence intensity after 70 h, while that of the untreated CdSe/CdS:Al, the treated CdSe/CdS and the CdSe/CdS QDs dropped to 65%, 45%, and 5%, respectively. Furthermore, we demonstrate that this method can be extended to other metal-doped QD systems, even including some inactive metals difficult to be oxidized spontaneously in an ambient atmosphere, which provides a new way to stabilize QDs for diverse optoelectronic applications.

The disposal of large amounts of biogas residue from anaerobically digested waste is a burden on environment protection. Porous biochars (BCs) were synthesized from biogas residue at three pyrolysis temperatures (300 °C, 550 °C, and 800 °C) and used to catalyze H2O2 for the degradation of benzene, toluene, ethylbenzene, xylene isomers (ortho, para, and meta), and styrene (BTEXS) to develop a new use for biogas residues. The prepared BCs were characterized through scanning electron microscopy, Brunauer–Emmett–Teller method, Fourier transform infrared spectrometry, and X-ray photoelectron spectroscopy. Results showed that BC800/H2O2 had the highest BTEXS degradation performance over 6 h. The degradation kinetic data were most consistent with the pseudo-second-order model. The different catalytic effect of the three BCs pyrolyzed at different temperatures were attributed to the dominant active sites (C–O/C–OH/C=C/C=O groups, pyridinic N, and graphitic N) that induced the production of reactive oxygen species (ROS). ROS-quenching experiments indicated that the degradation of BTEXS by BC300/H2O2, BC550/H2O2, and BC800/H2O2 involved ∙OH, ∙O2−, and 1O2. ∙OH was the dominant ROS in BC300/H2O2 and BC550/H2O2, and 1O2 was the dominant ROS in BC800/H2O2. Our findings provided new insight into the different catalytic mechanisms for BC production at different pyrolysis temperatures and demonstrated that a porous BC catalyst with high utilization value could be prepared from biogas residue and could hold considerable potential for application in BTEXS treatment in the future.

A colorimetric method is described for detection of the antibiotic ciprofloxacin (CIP) in aqueous solutions based on an unmodified CIP-aptamer and gold nanoparticles (AuNPs), which are regarded as a probe and an indicator, respectively. In the absence of CIP, aptamers can hybridize with poly dimethyl diallyl ammonium chloride (PDDA) to form a 'duplex' structure through electrostatic interactions, and AuNPs remain dispersed in solution, leading to a red solution and an obvious absorption peak at 520 nm. Contrarily, the CIP-aptamer can specifically bind to CIP after the introduction of CIP into the solution. Then PDDA is thus free to aggregate AuNPs. The solution turns blue from red accordingly, presenting a strong absorption at 650 nm. Hence, the concentration of CIP can be quantified through the changes of the absorption. This sensitive and selective colorimetric method for CIP detection has a good linear response (R = 0.9935) in the 20 to 300 nM CIP concentration range, with a limit of detection (LOD) of 0.215 nM. Importantly, the proposed aptasensor demonstrates great application potential in CIP determination in aqueous samples.