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32 results on '"Gupta, Prerak"'

8. List of Contributors

Author

Al Halabi, Noor A., primary, Alsalloum, Ghufran A., additional, Bhattacharjee, Promita, additional, Bommana, Murali M., additional, Cubayachi, Camila, additional, Dalmolin, Luciana F., additional, Evran, Serap, additional, Feksa, Luciane R., additional, Guaadaoui, Abdelkarim, additional, Gupta, Prerak, additional, Himri, Imane, additional, Joseph Christakiran, M., additional, Kopylov, Alexey, additional, Kumar, Anoop, additional, Kurbanoglu, Sevinc, additional, Lemos, Camila N., additional, Lopez, Renata F.V., additional, Luliński, Piotr, additional, Machado, Aline B., additional, Mandal, Biman B., additional, Muller, Cristina D., additional, Muthuraman, Arunachalam, additional, Nandi, Samit K., additional, Ozkan, Sibel A., additional, Ozyurt, Canan, additional, Pereira, Francieli, additional, Petrak, Karel, additional, Pillai, Jonathan, additional, Ramos, Danielle N., additional, Raut, Sangram, additional, Rech, Virgínia C., additional, Ribeiro, António J., additional, Santos, Ana C., additional, Sequeira, Joana A.D., additional, Serra, João, additional, Sobiech, Monika, additional, Troian, Eduardo A., additional, Ugurlu, Ozge, additional, Uslu, Bengi, additional, Veiga, Francisco, additional, Viegas, Fabian, additional, Yadav, Hemant K.S., additional, and Zavyalova, Elena, additional

Published
2018
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11. Deterministic Single Cell Encapsulation in Asymmetric Microenvironments to Direct Cell Polarity.

Author

Cho, Ik Sung, Gupta, Prerak, Mostafazadeh, Nima, Wong, Sing Wan, Saichellappa, Saiumamaheswari, Lenzini, Stephen, Peng, Zhangli, and Shin, Jae‐Won

Subjects
*CELL polarity, *CELL-matrix adhesions, *MESENCHYMAL stem cells, *CELL size, *CELL populations, *CELL adhesion
Abstract

Various signals in tissue microenvironments are often unevenly distributed around cells. Cellular responses to asymmetric cell‐matrix adhesion in a 3D space remain generally unclear and are to be studied at the single‐cell resolution. Here, the authors developed a droplet‐based microfluidic approach to manufacture a pure population of single cells in a microscale layer of compartmentalized 3D hydrogel matrices with a tunable spatial presentation of ligands at the subcellular level. Cells elongate with an asymmetric presentation of the integrin adhesion ligand Arg‐Gly‐Asp (RGD), while cells expand isotropically with a symmetric presentation of RGD. Membrane tension is higher on the side of single cells interacting with RGD than on the side without RGD. Finite element analysis shows that a non‐uniform isotropic cell volume expansion model is sufficient to recapitulate the experimental results. At a longer timescale, asymmetric ligand presentation commits mesenchymal stem cells to the osteogenic lineage. Cdc42 is an essential mediator of cell polarization and lineage specification in response to asymmetric cell‐matrix adhesion. This study highlights the utility of precisely controlling 3D ligand presentation around single cells to direct cell polarity for regenerative engineering and medicine. [ABSTRACT FROM AUTHOR]

Published
2023
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12. CCL2 loaded microparticles promote acute patency in silk-based vascular grafts implanted in rat aortae

Author

Lorentz, Katherine L., primary, Gupta, Prerak, additional, Shehabeldin, Mostafa S., additional, Cunnane, Eoghan M., additional, Ramaswamy, Aneesh K., additional, Verdelis, Konstantinos, additional, DiLeo, Morgan V., additional, Little, Steven R., additional, Weinbaum, Justin S., additional, Sfeir, Charles S., additional, Mandal, Biman B., additional, and Vorp, David A., additional

Published
2021
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16. Extracellular Vesicles Enhance the Remodeling of Cell-Free Silk Vascular Scaffolds in Rat Aortae

Author

Cunnane, Eoghan M, Lorentz, Katherine L, Ramaswamy, Aneesh K, Gupta, Prerak, Mandal, Biman B, O'Brien, Fergal J, Weinbaum, Justin S, and Vorp, David A

Subjects
vascular tissue engineering, mesenchymal stem cells, microvesicles, exosomes, aortic graft
Abstract

Vascular tissue engineering is aimed at developing regenerative vascular grafts to restore tissue function by bypassing or replacing defective arterial segments with tubular biodegradable scaffolds. Scaffolds are often combined with stem or progenitor cells to prevent acute thrombosis and initiate scaffold remodeling. However, there are limitations to cell-based technologies regarding safety and clinical translation. Extracellular vesicles (EVs) are nanosized particles released by most cell types, including stem and progenitor cells, that serve to transmit protein and RNA cargo to target cells throughout the body. EVs have been shown to replicate the therapeutic effect of their parent cells; therefore, EVs derived from stem or progenitor cells may serve as a more translatable, cell-free, therapeutic base for vascular scaffolds. Our study aims to determine if EV incorporation provides a positive effect on graft patency and remodelingin vivo. We first assessed the effect of human adipose-derived mesenchymal stem cell (hADMSC) EVs on vascular cells usingin vitrobioassays. We then developed an EV-functionalized vascular graft by vacuum-seeding EVs into porous silk-based tubular scaffolds. These constructs were implanted as aortic interposition grafts in Lewis rats, and their remodeling capacity was compared to that observed for hADMSC-seeded and blank (non-seeded) controls. The EV group demonstrated improved patency (100%) compared to the hADMSC (56%) and blank controls (82%) following eight weeksin vivo. The EV group also produced significantly more elastin (126.46%) and collagen (44.59%) compared to the blank group, while the hADMSC group failed to produce significantly more elastin (57.64%) or collagen (11.21%) compared to the blank group. Qualitative staining of the explanted neo-tissue revealed improved endothelium formation, increased smooth muscle cell infiltration, and reduced macrophage numbers in the EV group compared to the controls, which aids in explaining this group’s favorable pre-clinical outcomes.

Published
2020

29. Substrate stiffness does affect the fate of human keratinocytes

Author

Gupta, Prerak, primary, Hari Narayana S. N., Gautham, additional, Kasiviswanathan, Uvanesh, additional, Agarwal, Tarun, additional, K., Senthilguru, additional, Mukhopadhyay, Devdeep, additional, Pal, Kunal, additional, Giri, Supratim, additional, Maiti, Tapas K., additional, and Banerjee, Indranil, additional

Published
2016
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32. Fabrication of Small-Diameter Tubular Grafts for Vascular Tissue Engineering Applications Using Mulberry and Non-mulberry Silk Proteins.

Author

Gupta P and Mandal BB

Subjects
Fibroins, Silk, Tissue Engineering, Tissue Scaffolds, Morus
Abstract

Silk fibroin (SF) is a natural well-known biomaterial that has widely been explored for various tissue engineering applications with great success. Herein, we describe the methodology for fabricating two different types of tubular silk scaffolds aimed for vascular grafting. The first method emphasizes the use of very thin (10-15μm) silk films with unidirectional longitudinal micro-patterns, followed by their sequential rolling, which results in a multilayered tubular graft mimicking native-like cellular composition. The second method describes the fabrication of a bi-layered tubular scaffold comprising of a highly porous inner layer covered with an outer nanofibrous electrospun layer., (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2022
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