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Protein arginine methyltransferase 3 (PRMT3), a type I arginine methyltransferase is localized predominantly in the cytoplasm and regulates different cellular functions. Nevertheless, PRMT3 also exhibits regulatory functions in the nucleus by interacting with the liver X receptor alpha (LXRa) and catalyzes asymmetric dimethylation modifications at arginine 3 of histone 4 (H4R3me2a). However, very little is known about the regulation of the versatile global regulator PRMT3 and how PRMT3 is translocated to the nucleus. In this study, we identified ZNF200, a hitherto uncharacterized protein, as a potential binding partner of PRMT3 through yeast two-hybrid screening. We confirmed the interaction of PRMT3 with ZNF200 using immunoprecipitation and in vitro pull-down experiments. GST pull-down experiments and molecular docking studies revealed that the N-terminal zinc finger domain of PRMT3 binds to the C-terminal zinc finger regions of ZNF200. Furthermore, the evolutionary conservation of the Znf domain of PRMT3 correlates with the emergence of ZNF200 in mammals. We found that ZNF200 stabilizes PRMT3 by inhibiting its proteasomal degradation. ZNF200, a nuclear-predominant protein, promotes the nuclear translocation of PRMT3, leading to the global increase of H4R3me2a modifications. These findings imply that ZNF200 is a critical regulator of the steady-state levels and nuclear and epigenetic functions of PRMT3. [ABSTRACT FROM AUTHOR]

The study reports on the use of sol–gel technique to yield zircon type [Zr(1–0.1–x) GdxTi0.1] [(SiO4)1–x(PO4)x] solid solution. Titanium has been used as a mineralizer to trigger zircon formation while equimolar concentrations of Gd3+ and PO43− were added to determine their accommodation limits in the zircon structure. The crystallization of t‐ZrO2 as a dominant phase alongside the crystallization of m‐ZrO2 and zircon were detected at 1200°C while their further annealing revealed the formation of zircon as a major phase at 1300°C. Heat treatment at 1400°C revealed the formation of zircon‐type solid solution [Zr(1–0.1‐x)GdxTi0.1][(SiO4)1−x(PO4)x] comprising the accommodation of 10 mol.% of Gd3+/PO43− at the zircon lattice. Beyond 10 mol.% of Gd3+/PO43−, the crystallization of GdPO4 as a secondary phase is noticed. Structural analysis revealed the expansion of zircon lattice due to the simultaneous occupancy of Gd3+/PO43− for the corresponding Zr4+/SiO44− sites. The mechanical strength of single‐phase zircon solid solution was higher in comparison to that of multiphase materials, namely in the presence of GdPO4 formed as a secondary phase in samples with added equimolar Gd3+/PO43− contents beyond 10 mol.%. Nevertheless, the paramagnetic behavior of the samples demonstrated a steady surge as a function of enhanced Gd3+ content. [ABSTRACT FROM AUTHOR]

The crystallization of ZrSiO4 is generally accomplished by the addition of mineralizers into ZrO2–SiO2 binary oxides. The current investigation aimed to investigate the effect of adding calcium phosphates into ZrO2–SiO2 binary oxides on the yield of ZrSiO4. The concentration of calcium phosphate additions were varied to obtain ZrSiO4 that fetches improved mechanical and biological properties for application in hard tissue replacements. The findings highlight the significant role of Ca2+ and P5+ in triggering the ZrSiO4 formation via their accommodation at the Zr4+ and Si4+ sites. Especially, calcium phosphate additions trigger the t- → m-ZrO2 transition beyond 1000°C, which consequently reacts with SiO2 to promote ZrSiO4 formation. Calcium phosphates are accommodated at the lattice sites of ZrSiO4 with a maximum limit of 20 mol%, beyond which the crystallization of β-Ca3(PO4)2 is noticed. The optimum amount of 20 mol% of calcium phosphates displayed a better strength than that of all the investigated specimens. More than 80% of cell viability in MG-63 cells was invariably determined in all the calcium phosphate-added ZrSiO4 systems. [ABSTRACT FROM AUTHOR]

The study reports on the use of sol-gel technique to yield zircon type [Zr (1-0.1-x) GdxTi 0.1 ] [(SiO 4 ) 1-x (PO 4 ) x ] solid solution. Titanium has been used as a mineralizer to trigger zircon formation while equimolar concentrations of Gd 3+ and PO 4 3- were added to determine their accommodation limits in the zircon structure. The crystallization of t-ZrO 2 as a dominant phase alongside the crystallization of m-ZrO 2 and zircon were detected at 1200°C while their further annealing revealed the formation of zircon as a major phase at 1300°C. Heat treatment at 1400°C revealed the formation of zircon-type solid solution [Zr (1-0.1-x) Gd x Ti 0.1 ][(SiO 4 ) 1-x (PO 4 ) x ] comprising the accommodation of 10 mol.% of Gd 3+ /PO 4 3- at the zircon lattice. Beyond 10 mol.% of Gd 3+ /PO 4 3- , the crystallization of GdPO 4 as a secondary phase is noticed. Structural analysis revealed the expansion of zircon lattice due to the simultaneous occupancy of Gd 3+ /PO 4 3- for the corresponding Zr 4+ /SiO 4 4- sites. The mechanical strength of single-phase zircon solid solution was higher in comparison to that of multiphase materials, namely in the presence of GdPO 4 formed as a secondary phase in samples with added equimolar Gd 3+ /PO 4 3- contents beyond 10 mol.%. Nevertheless, the paramagnetic behavior of the samples demonstrated a steady surge as a function of enhanced Gd 3+ content., (© 2023 Wiley Periodicals LLC.)