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8 results on '"Héry, Mélanie"'

2. Low CCR5 expression protects HIV-specific CD4+ T cells of elite controllers from viral entry

Author

Claireaux, Mathieu, Robinot, Rémy, Kervevan, Jérôme, Patgaonkar, Mandar, Staropoli, Isabelle, Brelot, Anne, Nouël, Alexandre, Gellenoncourt, Stacy, Tang, Xian, Héry, Mélanie, Volant, Stevenn, Perthame, Emeline, Avettand-Fenoël, Véronique, Buchrieser, Julian, Cokelaer, Thomas, Bouchier, Christiane, Ma, Laurence, Boufassa, Faroudy, Hendou, Samia, Libri, Valentina, Hasan, Milena, Zucman, David, de Truchis, Pierre, Schwartz, Olivier, Lambotte, Olivier, and Chakrabarti, Lisa A.

Published
2022
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3. Hypomorphic pathogenic variant in SFTPBleads to adult pulmonary fibrosis

Author

Desroziers, Tifenn, Prévot, Grégoire, Coulomb, Aurore, Nau, Valérie, Dastot-Le Moal, Florence, Duquesnoy, Philippe, Héry, Mélanie, Le Borgne, Aurélie, Amselem, Serge, Legendre, Marie, and Nathan, Nadia

Abstract

Biallelic pathogenic variants in the surfactant protein (SP)-B gene (SFTPB) have been associated with fatal forms of interstitial lung diseases (ILD) in newborns and exceptional survival in young children. We herein report the cases of two related adults with pulmonary fibrosis due to a new homozygous SFTPBpathogenic variant, c.582G>A p.(Gln194=). In vitro transcript studies showed that this SFTPBsynonymous pathogenic variant induces aberrant splicing leading to three abnormal transcripts with the preservation of the expression of a small proportion of normal SFTPBtranscripts. Immunostainings on lung biopsies of the proband showed an almost complete loss of SP-B expression. This hypomorphic splice variant has thus probably allowed the patients’ survival to adulthood while inducing an epithelial cell dysfunction leading to ILD. Altogether, this report shows that SFTPBpathogenic variants should be considered in atypical presentations and/or early-onset forms of ILD particularly when a family history is identified.

Published
2023
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4. Specificities and Commonalities of Carbapenemase-Producing Escherichia coli Isolated in France from 2012 to 2015

Author

Patiño-Navarrete, Rafael, Rosinski-Chupin, Isabelle, Cabanel, Nicolas, Dieudonné Zongo, Pengdbamba, Héry, Mélanie, Oueslati, Saoussen, Girlich, Delphine, Dortet, Laurent, Bonnin, Rémy, Naas, Thierry, Glaser, Philippe, Écologie et Évolution de la Résistance aux Antibiotiques / Ecology and Evolution of Antibiotics Resistance (EERA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Saclay-Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes (IMVA-HB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, French National Reference Center for Antibiotic Resistance: Carbapenemase producing Enterobacteriaceae [Le Kremlin-Bicêtre], AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), This work was supported by grants from the French National Research Agency (ANR-10-LABX-62-IBEID, ANR-10-LABX-33, INCEPTION project (PIA/ANR-16-CONV-0005)) and from the European Union’s Horizon 2020 research and Innovation Program under grant agrement No 773830 (Project ARDIG, One Health EJP). Pengdbamba Dieudonné Zongo is a scholar of Ed525 CDV, Sorbonne Université, Paris., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-LABX-0033,LERMIT,Research Laboratory on Drugs and Therapeutic Innovation(2010), ANR-16-CONV-0005,INCEPTION,Institut Convergences pour l'étude de l'Emergence des Pathologies au Travers des Individus et des populatiONs(2016), European Project: 773830,H2020-SFS-2017-1,MedVetKlebs (a component of European Joint Programme One Health)(2018), Ecologie et Evolution de la Résistance aux Antibiotiques / Ecology and Evolution of Antibiotics Resistance (EERA), Institut Pasteur [Paris]-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPC), and European Project: 773830,H2020-SFS-2017-1,MedVetKlebs (a component of European Joint Programme One Health EJP)(2018)

Subjects
Carbapenem-Resistant Enterobacteriaceae, multidrug resistance, drug resistance evolution, Escherichia coli, genomics, Humans, emergence, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, France, surveillance studies, Escherichia coli Infections, Phylogeny, Research Article
Abstract

Carbapenemase-producing Escherichia coli (CP-Ec) represents a major public health threat with a risk of dissemination in the community as has occurred for lineages producing extended-spectrum β-lactamases. To characterize the extent of CP-Ec spread in France, isolates from screening and infection samples received at the French National Reference Center (F-NRC) laboratory for carbapenemase-producing Enterobacterales were investigated. A total of 691 CP-Ec isolates collected between 2012 and 2015 and 22 isolates collected before 2012 were fully sequenced. Analysis of their genome sequences revealed some disseminating multidrug-resistant (MDR) lineages frequently acquiring diverse carbapenemase genes mainly belonging to clonal complex 23 (CC23) (sequence type 410 [ST410]) and CC10 (ST10 and ST167) and sporadic isolates, including rare ST131 isolates (n = 17). However, the most represented sequence type (ST) was ST38 (n = 92) with four disseminated lineages carrying blaOXA-48-like genes inserted in the chromosome. Globally, the most frequent carbapenemase gene (n = 457) was blaOXA-48. It was also less frequently associated with MDR isolates being the only resistance gene in 119 isolates. Thus, outside the ST38 clades, its acquisition was frequently sporadic with no sign of dissemination, reflecting the circulation of the IncL plasmid pOXA-48 in France and its high frequency of conjugation. In contrast, blaOXA-181 and blaNDM genes were often associated with the evolution of MDR E. coli lineages characterized by mutations in ftsI and ompC. IMPORTANCE Carbapenemase-producing Escherichia coli (CP-Ec) might be difficult to detect, as MICs can be very low. However, their absolute number and their proportion among carbapenem-resistant Enterobacterales have been increasing, as reported by WHO and national surveillance programs. This suggests a still largely uncharacterized community spread of these isolates. Here, we have characterized the diversity and evolution of CP-Ec isolated in France before 2016. We show that carbapenemase genes are associated with a wide variety of E. coli genomic backgrounds and a small number of dominant phylogenetic lineages. In a significant proportion of CP-Ec, the most frequent carbapenemase gene blaOXA-48, was detected in isolates lacking any other resistance gene, reflecting the dissemination of pOXA-48 plasmids, likely in the absence of any antibiotic pressure. In contrast, carbapenemase gene transfer may also occur in multidrug-resistant E. coli, ultimately giving rise to at-risk lineages encoding carbapenemases with a high potential of dissemination.

Published
2022

5. Specificities and commonalities of carbapenemase producing Escherichia coli isolated in France from 2012 to 2015

Author

Patiño-Navarrete, Rafael, primary, Rosinski-Chupin, Isabelle, additional, Cabanel, Nicolas, additional, Zongo, Pengdbamba Dieudonné, additional, Héry, Mélanie, additional, Oueslati, Saoussen, additional, Girlich, Delphine, additional, Dortet, Laurent, additional, Bonnin, Rémy A, additional, Naas, Thierry, additional, and Glaser, Philippe, additional

Published
2021
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6. Functional assessment and phenotypic heterogeneity of SFTPA1 and SFTPA2 mutations in interstitial lung diseases and lung cancer

Author

Legendre, Marie, primary, Butt, Afifaa, additional, Borie, Raphaël, additional, Debray, Marie-Pierre, additional, Bouvry, Diane, additional, Filhol-Blin, Emilie, additional, Desroziers, Tifenn, additional, Nau, Valérie, additional, Copin, Bruno, additional, Dastot-Le Moal, Florence, additional, Héry, Mélanie, additional, Duquesnoy, Philippe, additional, Allou, Nathalie, additional, Bergeron, Anne, additional, Bermudez, Julien, additional, Cazes, Aurélie, additional, Chene, Anne-Laure, additional, Cottin, Vincent, additional, Crestani, Bruno, additional, Dalphin, Jean-Charles, additional, Dombret, Christine, additional, Doray, Bérénice, additional, Dupin, Clairelyne, additional, Giraud, Violaine, additional, Gondouin, Anne, additional, Gouya, Laurent, additional, Israël-Biet, Dominique, additional, Kannengiesser, Caroline, additional, Le Borgne, Aurélie, additional, Leroy, Sylvie, additional, Longchampt, Elisabeth, additional, Lorillon, Gwenaël, additional, Nunes, Hilario, additional, Picard, Clément, additional, Reynaud-Gaubert, Martine, additional, Traclet, Julie, additional, de Vuyst, Paul, additional, Coulomb L'Hermine, Aurore, additional, Clement, Annick, additional, Amselem, Serge, additional, and Nathan, Nadia, additional

Published
2020
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8. Specificities and Commonalities of Carbapenemase-Producing Escherichia coli Isolated in France from 2012 to 2015.

Author

Patiño-Navarrete R, Rosinski-Chupin I, Cabanel N, Zongo PD, Héry M, Oueslati S, Girlich D, Dortet L, Bonnin RA, Naas T, and Glaser P

Subjects
Humans, Escherichia coli, Phylogeny, France, Escherichia coli Infections epidemiology, Carbapenem-Resistant Enterobacteriaceae
Abstract

Carbapenemase-producing Escherichia coli (CP- Ec ) represents a major public health threat with a risk of dissemination in the community as has occurred for lineages producing extended-spectrum β-lactamases. To characterize the extent of CP- Ec spread in France, isolates from screening and infection samples received at the French National Reference Center (F-NRC) laboratory for carbapenemase-producing Enterobacterales were investigated. A total of 691 CP- Ec isolates collected between 2012 and 2015 and 22 isolates collected before 2012 were fully sequenced. Analysis of their genome sequences revealed some disseminating multidrug-resistant (MDR) lineages frequently acquiring diverse carbapenemase genes mainly belonging to clonal complex 23 (CC23) (sequence type 410 [ST410]) and CC10 (ST10 and ST167) and sporadic isolates, including rare ST131 isolates ( n  = 17). However, the most represented sequence type (ST) was ST38 ( n  = 92) with four disseminated lineages carrying bla OXA-48-like genes inserted in the chromosome. Globally, the most frequent carbapenemase gene ( n  = 457) was bla OXA-48 . It was also less frequently associated with MDR isolates being the only resistance gene in 119 isolates. Thus, outside the ST38 clades, its acquisition was frequently sporadic with no sign of dissemination, reflecting the circulation of the IncL plasmid pOXA-48 in France and its high frequency of conjugation. In contrast, bla OXA-181 and bla NDM genes were often associated with the evolution of MDR E. coli lineages characterized by mutations in ftsI and ompC. IMPORTANCE Carbapenemase-producing Escherichia coli (CP- Ec ) might be difficult to detect, as MICs can be very low. However, their absolute number and their proportion among carbapenem-resistant Enterobacterales have been increasing, as reported by WHO and national surveillance programs. This suggests a still largely uncharacterized community spread of these isolates. Here, we have characterized the diversity and evolution of CP- Ec isolated in France before 2016. We show that carbapenemase genes are associated with a wide variety of E. coli genomic backgrounds and a small number of dominant phylogenetic lineages. In a significant proportion of CP- Ec , the most frequent carbapenemase gene bla OXA-48 , was detected in isolates lacking any other resistance gene, reflecting the dissemination of pOXA-48 plasmids, likely in the absence of any antibiotic pressure. In contrast, carbapenemase gene transfer may also occur in multidrug-resistant E. coli, ultimately giving rise to at-risk lineages encoding carbapenemases with a high potential of dissemination.

Published
2022
Full Text
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