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20. Union Schweizerischer Gesellschaften für experimentelle Biologie Berichte der 2. Jahresversammlung: Fribourg, 23.–24. Mai 1970

Author

Akert, K., Kawana, E., Pfenninger, K., Moor, H., Sandri, C., Arczynska, W., Held, D. R., Haab, P., Boudry, J. F., Rioch-Ramel, F., Burri, P. H., Weibel, E. R., Cerretelli, P., di Prampero, P. E., Piiper, J., Chinet, A., Farber, J. P., Bloom, D. A., Farhi, L. E., Chmouliovsky, M., Churchill, P. C., Dolivo, M., Koelle, G. B., Geelhaar, A., Robert, M., Handwerker, H. O., Wettstein, A., Hösli, L., TebĒcis, A. K., Schönwetter, H. P., Andrès, P. F., Hösli, E., Jirounek, P., Straub, R. W., Kalix, P., Kelly, J. S., Dreifuss, J. J., Jéquier, E., Krayenbühl, H. P., Mehmel, H., Rutishauser, W., Meier, R. E., Dalderup, J., Cuénod, M., Perisic, M., Niemeyer, G., Forssmann, W. G., Oetliker, H., McGuigan, J. A. S., Parmeggiani, P. L., Franzini, C., Scheid, P., Roch-Ramel, F., Jotterand, N., Scherrer, M., Bitterli, J., Siegwart, B., Steiner, C. A., Tomori, Z., Trachtenberg, M. C., Kornblith, P., Häuptli, J., Braun, R., Zellweger, A., Bron, Th., Rous, S., Chmelar, M., Chmelarova, M., Favarger, P., Cueni, L., Schoenenberger, G. A., Allgöwer, M., Schonbach, J., Exton, J. H., Miller, Jr., T. B., Park, C. R., Fellay, G., Portmann, P., GonÇalves, J., Honegger, C. G., Schardt, M., Hecker, H., Marx, W., Shoyab, M., Murer, H., Schatzmann, H. J., Schenker, T. M., von Wartburg, J. P., Schindler, R., Schaer, J. C., Grieder, A., Stauffacher, W., Amherdt, M., Cameron, D., Burr, I., Balant, L., Orci, L., Stucki, J. W., Walter, P., Touabi, M., Jeanrenaud, B., Wacker, H., Lehky, P., Stein, E., Anabitarte, M., Winterhalter, K. H., Tuchschmid, P., Briehl, R. W., Brunori, M., Amiconi, G., Antonini, E., Wyman, J., Anner, Béatrice, Ferrero, J., Schorderet, M., Burkard, W. P., Lengsfeld, H., Gey, K. F., Haefely, W., Corthay, J., Benakis, A., Glasson, B., Haeusler, G., Somlyo, A. V., Somlyo, A. P., Heinrich, U., Langemann, H., Lichtensteiger, W., Iynedjian, P. B., Peters, G., Jalfre, M., Monachon, M. A., Lazáry, S., Stähelin, H., Minder, R., Bickel, M. H., Müller, A. H., Brunner, H., Pletscher, A., Da Prada, M., Berneis, K. H., Thomasset, M., Black, L. W., Ahmad-Zadeh, C., Dubochet, J., Ducommun, M., Kellenberger, E., Wacher, Ph., Tesi, D., Daldrup, J., Gautier, A., Schreyer, M., Cogliati, R., Gautschi, J. R., Goodman, H. M., Billeter, M. A., Hindley, J., Weissmann, C., Granboulan, P., Hess, M. W., Sordat, B., Cottier, H., Joel, D. D., Kellenberger-van der Kamp, C., Kolakofsky, D., Mechler, B., Mach, B., Mira-Moser, F., Schiller, P., Schwyzer, R., Scholl, A., Eppenberger, H. M., Séchaud, J., Staehelin, M., Wehrli, W., Yanagida, M., and Ahmad-Zadeh, G.

Published
1970
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47. Light and electron microscopic localization of GABAA-receptors on cultured cerebellar granule cells and astrocytes using immunohistochemical techniques

Author

Hansen, Gert Helge, Hösli, E, Belhage, B, Schousboe, A, Hösli, L, Hansen, Gert Helge, Hösli, E, Belhage, B, Schousboe, A, and Hösli, L

Abstract

Udgivelsesdato: 1991-Mar, GABAA-receptors were localized in explant cultures of rat cerebellum and in dissociated primary cultures of rat cerebellar granule cells and rat cerebellar astrocytes using the monoclonal antibody bd-17 directed against the beta-subunit of the GABAA/benzodiazepine/chloride channel complex. At the light microscope level specific staining of GABAA-receptors was localized in various types of neurones in explant cultures of rat cerebellum using the indirect peroxidase-antiperoxidase (PAP) technique, whereas no specific staining was found in astrocytes. At the electron microscope level labeling of GABAA-receptors was observed in the plasma membrane of both the cell bodies and processes in dissociated primary cultures of cerebellar granule cells using an indirect preembedding immunogold staining technique which in contrast to the classical PAP technique allows quantitative estimations to be performed. Quantification of the labeling intensity revealed a higher concentration of GABAA-receptors per microns plasma membrane in the cell bodies than in the processes. In discrete areas an extremely high density of the GABAA-receptors was observed. No specific labeling of GABAA-receptors was observed in dissociated primary cultures of cerebellar astrocytes.

Published
1991

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