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1. Structural analysis of mixed alkali borosilicate glasses containing Cs+ and Na+ using strong magnetic field magic angle spinning nuclear magnetic resonance

Author

S. Kaneko, Y. Tokuda, Y. Takahashi, H. Masai, and Y. Ueda

Subjects
NMR, Borosilicate glass, Mixed alkali effect, Cs, Na, Clay industries. Ceramics. Glass, TP785-869
Abstract

We have investigated the local structure of alkali atoms in mixed alkali silicate, borate, and borosilicate glasses, which contain Cs+ and Na+, using strong magnetic field magic angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy of 133Cs and 23Na. The spectral peaks of 133Cs in borosilicate (Si:B = 1:1) and Si-rich borosilicate (Si:B = 2:1) glasses shifted to upfield with increasing Cs+/(Na+ + Cs+) ratio, which implies that the coordination number of Cs+ decreased as in the case of silicate and borate glasses. However, this trend was not observed in the 23Na spectra of either borosilicate glass. This might be because the chemical shift of 23Na in borosilicate glass is strongly affected by nearby species such as Si or B, and not by the coordination number of Na+.

Published
2017
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2. Structural analysis of alkali cations in mixed alkali silicate glasses by 23Na and 133Cs MAS NMR

Author

T. Minami, Y. Tokuda, H. Masai, Y. Ueda, Y. Ono, S. Fujimura, and T. Yoko

Subjects
Mixed alkali silicate glasses, Solid-state NMR, Coordination number, Mixed alkali effect, Clay industries. Ceramics. Glass, TP785-869
Abstract

We report the structural analysis of Na+ and Cs+ in sodium cesium silicate glasses by using 23Na and 133Cs magic-angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy. In the NMR spectra of cesium silicate crystals, the peak position shifted to higher magnetic field for structures with larger Cs+ coordination numbers and to lower magnetic field for smaller Cs+ coordination numbers. The MAS NMR spectra of xNa2O-yCs2O-2SiO2 (x = 0, 0.2, 0.33, 0.5, 0.66, 0.8, 1.0; x + y = 1) glass reveal that the average coordination number of both the alkali cations decreases with increasing Cs+/(Na+ + Cs+) ratio. In addition, the coordination number of Na+ in xNa2O-yCs2O-2SiO2 glass is smaller than that of Cs+. This difference between the average coordination numbers of the alkali cations is considered to be one structural reason of the mixed alkali effect.

Published
2014
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3. Poster Session 2: Monday 4 May 2015, 08:00-18:00 * Room: Poster Area

Author

S. E. Bouyoucef, V. Uusitalo, V. Kamperidis, M. De Graaf, T. Maaniitty, I. Stenstrom, A. Broersen, A. Scholte, A. Saraste, J. Bax, J. Knuuti, T. Furuhashi, M. Moroi, T. Awaya, H. Masai, M. Minakawa, T. Kunimasa, H. Fukuda, K. Sugi, A. Berezin, A. Kremzer, O. Clerc, B. Kaufmann, M. Possner, R. Liga, J. Vontobel, F. Mikulicic, C. Graeni, D. Benz, P. Kaufmann, R. Buechel, M. Ferreira, M. Cunha, A. Albuquerque, D. Ramos, G. Costa, J. Lima, M. Pego, A. Peix, L. Cisneros, L. Cabrera, K. Padron, L. Rodriguez, F. Heres, R. Carrillo, E. Mena, Y. Fernandez, E. Huizing, J. Van Dijk, J. Van Dalen, J. Timmer, J. Ottervanger, C. Slump, P. Jager, S. Venuraju, A. Jeevarethinam, A. Yerramasu, S. Atwal, V. Mehta, A. Lahiri, A. Arjonilla Lopez, M. J. Calero Rueda, G. Gallardo, J. Fernandez-Cuadrado, D. Hernandez Aceituno, J. Sanchez Hernandez, H. Yoshida, A. Mizukami, A. Matsumura, O. Smettei, R. Abazid, S. Sayed, A. Mlynarska, R. Mlynarski, K. Golba, M. Sosnowski, S. Winther, M. Svensson, H. Jorgensen, K. Bouchelouche, L. Gormsen, N. Holm, H. Botker, P. Ivarsen, M. Bottcher, C. M. Cortes, E. Aramayo G, M. Daicz, J. Casuscelli, E. Alaguibe, A. Neira Sepulveda, M. Cerda, G. Ganum, M. Embon, J. Vigne, B. Enilorac, A. Lebasnier, L. Valancogne, D. Peyronnet, A. Manrique, D. Agostini, D. Menendez, S. Rajpal, C. Kocherla, M. Acharya, P. Reddy, I. Sazonova, Y. Ilushenkova, R. Batalov, Y. Rogovskaya, Y. Lishmanov, S. Popov, N. Varlamova, S. Prado Diaz, C. Jimenez Rubio, D. Gemma, E. Refoyo Salicio, S. Valbuena Lopez, M. Moreno Yanguela, M. Torres, M. Fernandez-Velilla, J. Lopez-Sendon, G. Guzman Martinez, A. Puente, S. Rosales, C. Martinez, M. Cabada, G. Melendez, R. Ferreira, A. Gonzaga, J. Santos, S. Vijayan, S. Smith, M. Smith, R. Muthusamy, Y. Takeishi, M. Oikawa, J. L. Goral, J. Napoli, O. Montana, A. Damico, M. Quiroz, P. Forcada, J. Schmidberg, N. Zucchiatti, D. Olivieri, A. Dumo, S. Ruano, R. Rakhit, J. Davar, D. Nair, M. Cohen, D. Darko, S. Yokota, A. Maas, M. Mouden, S. Knollema, S. Sanja Mazic, B. Lazovic, M. Marina Djelic, J. Jelena Suzic Lazic, T. Tijana Acimovic, M. Milica Deleva, Z. Vesnina, N. Zafrir, T. Bental, I. Mats, A. Solodky, A. Gutstein, Y. Hasid, D. Belzer, R. Kornowski, R. Ben Said, N. Ben Mansour, H. Ibn Haj Amor, C. Chourabi, A. Hagui, W. Fehri, H. Hawala, Z. Shugushev, A. Patrikeev, D. Maximkin, A. Chepurnoy, V. Kallianpur, A. Mambetov, G. Dokshokov, A. Teresinska, O. Wozniak, A. Maciag, J. Wnuk, A. Dabrowski, A. Czerwiec, J. Jezierski, K. Biernacka, J. Robinson, J. Prosser, G. Cheung, S. Allan, G. Mcmaster, S. Reid, A. Tarbuck, W. Martin, R. Queiroz, A. Falcao, M. Giorgi, R. Imada, S. Nogueira, W. Chalela, R. Kalil Filho, W. Meneghetti, V. Matveev, A. Bubyenov, V. Podzolkov, V. Baranovich, A. Faibushevich, Y. Kolzhecova, O. Volkova, J. Fernandez, G. Lopez, M. Dondi, D. Paez, C. Butcher, E. Reyes, M. Al-Housni, R. Green, H. Santiago, F. Ghiotto, S. Hinton-Taylor, A. Pottle, M. Mason, S. Underwood, I. Casans Tormo, R. Diaz-Exposito, E. Plancha-Burguera, K. Elsaban, H. Alsakhri, K. Yoshinaga, N. Ochi, Y. Tomiyama, C. Katoh, M. Inoue, M. Nishida, E. Suzuki, O. Manabe, Y. Ito, N. Tamaki, A. Tahilyani, F. Jafary, H. Ho Hee Hwa, S. Ozdemir, B. Kirilmaz, A. Barutcu, Y. Tan, F. Celik, S. Sakgoz, M. Cabada Gamboa, A. Puente Barragan, N. Morales Vitorino, M. Medina Servin, C. Hindorf, S. Akil, F. Hedeer, J. Jogi, H. Engblom, V. Martire, E. Pis Diez, M. Martire, D. Portillo, C. Hoff, A. Balche, J. Majgaard, L. Tolbod, H. Harms, J. Soerensen, J. Froekiaer, F. Nudi, G. Neri, E. Procaccini, A. Pinto, M. Vetere, G. Biondi-Zoccai, J. Soares, R. Do Val, M. Oliveira, J. Meneghetti, Y. Tekabe, T. Anthony, Q. Li, A. Schmidt, L. Johnson, M. Groenman, M. Tarkia, M. Kakela, P. Halonen, T. Kiviniemi, M. Pietila, S. Yla-Herttuala, A. Roivainen, S. Nekolla, S. Swirzek, T. Higuchi, S. Reder, S. Schachoff, M. Bschorner, I. Laitinen, S. Robinson, B. Yousefi, M. Schwaiger, T. Kero, L. Lindsjo, G. Antoni, P. Westermark, K. Carlson, G. Wikstrom, J. Sorensen, M. Lubberink, F. Rouzet, T. Cognet, K. Guedj, M. Morvan, F. El Shoukr, L. Louedec, C. Choqueux, A. Nicoletti, D. Le Guludec, A. Jimenez-Heffernan, F. Munoz-Beamud, E. Sanchez De Mora, C. Borrachero, C. Salgado, C. Ramos-Font, J. Lopez-Martin, M. Hidalgo, R. Lopez-Aguilar, E. Soriano, A. Okizaki, M. Nakayama, S. Ishitoya, J. Sato, K. Takahashi, I. Burchert, F. Caobelli, T. Wollenweber, M. Nierada, J. Fulsche, C. Dieckmann, F. Bengel, S. Shuaib, D. Mahlum, S. Port, E. Refoyo, E. Cuesta, G. Guzman, T. Lopez, S. Valbuena, S. Del Prado, M. Moreno, M. Harbinson, L. Donnelly, A. J. Einstein, L. L. Johnson, A. J. Deluca, A. C. Kontak, D. W. Groves, J. Stant, T. Pozniakoff, B. Cheng, L. E. Rabbani, S. Bokhari, C. Schuetze, S. Aguade-Bruix, M. Pizzi, G. Romero-Farina, M. Terricabras, D. Villasboas, J. Castell-Conesa, J. Candell-Riera, S. Brunner, L. Gross, A. Todica, S. Lehner, A. Di Palo, A. Niccoli Asabella, C. Magarelli, A. Notaristefano, C. Ferrari, G. Rubini, A. Sellem, S. Melki, W. Elajmi, H. Hammami, M. Ziadi, J. Montero, J. Ameriso, R. Villavicencio, T. F. Benito Gonzalez, A. Mayorga Bajo, R. Gutierrez Caro, M. Rodriguez Santamarta, L. Alvarez Roy, E. Martinez Paz, C. Barinaga Martin, J. Martin Fernandez, D. Alonso Rodriguez, I. Iglesias Garriz, S. Rosillo, S. Taleb, G. Cherkaoui Salhi, Y. Regbaoui, M. Ait Idir, A. Guensi, C. E. Martin Lopez, and M. Castano Ruiz

Subjects
Medical education, business.industry, Medicine, Radiology, Nuclear Medicine and imaging, General Medicine, Session (computer science), Cardiology and Cardiovascular Medicine, business
Published
2015
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4. Poster session: Dobutamine stress echo

Author

C. Vizza, X. Jeanrenaud, M. Satendra, L. Monti, A. Kovacs, D. Vanoli, V. Charles, A. Kardos, M. Chiarlo, C. Gruner, C. Monaco, A. Kuch-Wocial, K. Mizia-Stec, L. Sargento, D. Kautznerova, N. Resseguier, G. Tamborini, C. Cruz, P. Jurzak, A. Cogo, E. Lira, D. Al Mesned, Y. Aizawa, A. Chmiel, R. Corti, K. Kim, G. N. Elkilany, M. Haarman, R. Badagliacca, R. Weber, R. Bruno, L. Di Pino, I. Kaplanis, D. Kalimanovska-Ostric, D. Tsounis, M. Varoudi, H. Yoon, P. Goncalves, G. Mpitsios, I. Garcia-Lunar, P. Min, R. Mogelvang, K. Gieszczyk-Strozik, A. Blundo, A. Tarr, E. W. Remme, David Garcia-Dorado, V. Petronilli, A. Patel, C. Sousa, R. M. Lang, J. Mcghie, V. Monivas Palomero, C. Nomura, H. Yoshikawa, N. Lagopati, M. Gomberg-Maitland, R. Kalil, H. K. Jeon, K. Mrabet, A. Riberi, C. Zito, A. Khalatbari, D. Tarasov, L. Fusini, P. Marques, S. Hassantash, I. Zimbarra Cabrita, M. Francone, A. Germain, A. Theron, J. Sousa, A. Kantorova, F. Collart, C. J. Vrints, A. Forteza, C. Tamburino, D. Cerna, S. Buccheri, M. Taborsky, I. P. Monte, F. Elmkies, A. Castro Beiras, S. Ranjbar, A. Perpinia, O. A. Tolba, R. Pretre, T. Chua, F. Fedele, M. Calcagnino, D. Dragulescu, M. Greutmann, M. Pepi, M. Bartesaghi, S. Urheim, R. Muscariello, F. Ben Moussa, W. Saib, M. Thameur, J. Ternacle, V. Matzraki, M. Ghannouchi, G. Kocabay, A. Margulescu, R. Sicari, R. Ippolito, M. Kloeckner, A. Toth, J. Gonzalez Mirelis, K. Sugi, M. Geleijnse, T. Otsuka, A. Hervold, S. Benyoussef, B. Basnyat, H. Suomi, L. Gargani, M. Stosic, P. Monney, J. Segovia Cubero, M. Karvandi, P. Sousa, J. Gonzalez-Mirelis, P. Caso, M. Murata, M. Vieira, C. Fulcheri, M. Júlia Maciel, P. Garcia-Pavia, M. Bobbo, J. Sun, B. Nardi, V. Pyrgakis, J. W. Kim, F. Alamanni, D. Ozel, A. Cordovil, S. Cimino, S. Papa, A. Carro, E. Leiballi, S. Karakas, J. Cho, C. Mornos, H. Masai, M. D'angelo, S. Mingo Santos, J. Kang, N. Nishiyama, J. Brugada, W. Tsang, Y. Yoon, B. Herzog, F. Dominguez Rodriguez, G. Ertl, E.R. Valsangiacomo Buechel, H. Shin, M. Palinsky, P. Gaudron, O. Gaemperli, A. Bouzas Mosquera, R. Bogle, J. Rodriguez-Palomares, N. Liel-Cohen, J. Burrello, M. Henein, H. Yilmaz, M. Laine, C. Foucher, K. Tanimoto, P. Schiattarella, G. Teixido, V. Schiano Lomoriello, M. R. El-Shanshory, N. Lousada, T. Minarik, F. Machado, G. Hashimoto, Y. Ishikawa, P. Atkinson, I. Zairi, B. Lee, V. Lanska, T. Biering-Sorensen, D. Vinereanu, H. Dores, M. Nakamura, R. Kockova, A. Chenzbraun, A. Manrique, N. A. Garcia, C. Zimmermann, L. Carpinteiro, H. Youn, J. Guimaraes, P. Meimoun, M. Mohammed, A. Gaspar, G. Styczynski, M. Castella, R. Esposito, A. Karavidas, F. Tosello, J. Mills, J. E. Sanderson, Y. Lau, D. Lee, C. Chin, M. Dostanic, D. Liu, P. Lupinek, T. Sato, M. Lewis, M. Reali, E. Cervesato, A. Apor, D. Sharif, S. Leggio, T. Ono, S. Wos, S. Kadrabulatova, S. Miyoshi, B. Milakovic, M. Gonzalez-Alujas, Y. Y. Lam, W. Tietge, M. Tramarin, L. Balzarini, E.-S. El-Hawary, G. E. Nagib Elkilany, P. Lim, P. Lindqvist, F. Veronesi, G. Flahaut, M. Thomas, A. Redheuil, Y. Ahn, M. Galderisi, M. Cavero Gibanel, J. Roquette, G. D. Lenders, F. Cicogna, P. Nihoyannopoulos, S. Taddei, C. Shahla, O. Mirea, A. Aleixo, E. Altekin, A. Milan, J. Roncalli, V. Mor-Avi, P. Crapanzano, S. Wang, A. Rodrigues, D. De Palma, M. Sitges, J. Peteiro, G. Maldonado, A. Nagy, J. Wang, M. Miglioranza, M. J. Claeys, J. Kluin, R. H. Strasser, J. Masura, B. Pezzuto, S. Aakhus, M C De Knegt, F. Broullon, N. Bhave, Y. Kusunose, R. Domburg, S. Moral Torres, J. Song, G. Carlomagno, P. Carrilho-Ferreira, A. Mornos, K. Sedlacek, Y. Villain, S. Arapi, M. Segetova, T. Le Tourneau, M. Kucuk, H. Tsuruta, J.-L. Monin, L. Badano, C. Mueller, C. Jorge, J. Kautzner, U. Schubert, L. Zhong, B. Suran, J. Clerc, I. Demir, S. Chamuleau, P. Tittel, E. Boussabah, P. Punjabi, L. Guimaraes, C. Magnino, B. Delasalle, D. Leone, J. Gruenenfelder, H. Blafield, F. Thuny, J. Jensen, J. Silva Cardoso, S. Stoebe, S. Sioua, K. Fukuda, M. Nocioni, P. Linden, V. Sanchez, D. Silva, V. Sikula, F. Pizzino, L. Kryze, A. Lebreiro, M. Deljanin-Ilic, A. Arsenio, S. Takatsuki, M. Kaldararova, A. Sikora-Puz, M. Cinello, S. Naffati, M. Pirscova, V. Lisignoli, A. Hagendorff, T. Iwaki, M. Niemann, E. Rees, U. Rosenschein, V. Vrsanska, C. A. Szmigielski, G. L. Nicolosi, G. Di Bella, D. Pfeiffer, R. Giorgi, K. Korpi, E. Paucca, M. Sanchez Garcia, S. Kammoun, M. Rodolico, Arturo Evangelista, I. Baka, J. Lima, C. Yu, B. Hong, C. Fischer, P. Morera, F. C. Tanner, R. Manganaro, M. Mezzapesa, B. Seifert, A. Berruezo, H. Guterman, K. Sveric, U. Wiklund, R. Sant'anna, R. Piazza, L. Oreto, L. Mont, J. Rosso, B. P. Paelinck, S. Severino, J. Park, S. Morhy, S. Mingo, A. Ledakowicz-Polak, L. Arcari, E. E.-S. El-Hawary, E. Caiani, R. Fabregas Casal, A. Bensaid, N. A. E.-A. El-Shitany, F. Veglio, L. Gutierrez, R. Massey, R. Mimo, A. Yanikoglu, A. Al Akhfash, J. Rodriguez Garrido, S. Kovalova, N. Patrascu, M. Liu, B. Bijnens, J.-L. Dubois-Rande, M. Suzuki, I. Garcia Lunar, D. Muraru, S. Iwanaga, R. Borras, R. Karpov, T. Nastasovic, T. Gonzalez-Alujas, M. Jasinski, H. Marques, W. Voelker, D. Maan Hasson, K. Murbrach, J. Yoon, M. Cusma-Piccione, S. Carerj, E. Hopp, D. A. Rees, M. Zielinska, M. Forkmann, M. Sotiropoulos, I. Zegri, Y. Neuder, V. Hraska, R. Iengo, I. Losano, P. Gripari, J. Avierinos, I. Simkova, M. Yaacobi, F. Weidemann, C. Sordelli, H. Jeong, T. Osaki, M. Kubanek, R. Sharma, M. Yamamoto, D. Bettex, J. Sivertsen, G. Bruno, A E Van Den Bosch, P. Kracht, P. L. Van Herck, J. Roos-Hesselink, D. Cozma, E. Teiger, L. Said, B. Freed, A. Loimaala, T. Pinho, L. Pomidori, A. Mantovani, A. Santoro, R. Kadour, R. Calabro, S. Rim, L. Sim, B. Merkely, P. Gueret, R. Jansen, G. Curatolo, C.H. Attenhofer Jost, C. Gambardella, V. Jarvinen, P. Hol, D. Mihalcea, P. Sogaard, D. Peluso, O. Kretschmar, F. Fang, H. Cuellar, F. Maffessanti, R Palma Dos Reis, J. Grapsa, A. Sharif-Rasslan, H. Kwon, P. Novak, R. Gallet, C. Sportouch, O. Enescu, H. Chung, M. Valtonen, D. Dawson, A. G. Fraser, M. Lyra Georgosopoulou, Q. Shang, V. Leonelli, L. Agati, A. Khalil, G. Habib, M. Cavero, A. Ionac, M. Florescu, S. Pescariu, L. Ascione, M. Carmo, A. Marouen, A. D'Andrea, S. Champagne, S. Iliceto, J. P. Halcox, M. Mizia, Z. Gasior, M. Cramer, S F de Marchi, S. Goncalves, L. Dal Bianco, N. Cortez-Dias, U. Richter, I. Santos, U. Naslund, E. Gonzalez Lopez, M. Rover, H. Vago, A. E.-A. El-Shitany, G Teixido Tura, M. Sramko, J. Necas, S. Fennira, M. Gomez Bueno, L. Zakhama, L. Costanzo, H. Zemir, F. Dunstan, R. Pecoraro, R. Hocking, L. Gabrielli, R. Tan, J. Tintera, L. Pratali, V. Monivas, B. Bouzas Zubeldia, B. Segafredo, T. Leiria, R. Mincu, A. Kaczynska, L. Petrescu, J. M. Bosmans, A. Ben Yaala, A. Ploussi, K. Hu, Z. Frikha, L. De Luca, E. Choi, J. Yanez Wonenburger, I. Serbanoiu, C. Iacoboni, J. Trochu, S. Montserrat, X. Luo, E. Pavlukova, D. Martinez Ruiz, G. Lazaros, B. Tan, D. Hudziak, J. Petrovicova, S. Herrmann, P. Biaggi, E. Picano, I. E. Rodrigus, Y. Lam, M. Jeong, M. Fedorco, P. Beltran Correas, C. Felix, L. Polak, C. Wunderlich, S. Hohlfeld, S. Tripepi, M. Haberka, R. Poscia, L. Halmai, A. Luycx-Bore, K. Tunstall, D. Becker, H. Dave, P. Lemarchand, and M. Carvalho

Subjects
Leading edge, business.industry, Reference values, Healthy subjects, Medicine, Radiology, Nuclear Medicine and imaging, Geometry, General Medicine, Edge (geometry), Cardiology and Cardiovascular Medicine, business
Published
2012
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5. Abstracts

Author

V. Dunet, A. Dabiri, G. Allenbach, A. Goyeneche Achigar, B. Waeber, F. Feihl, R. Heinzer, J. O. Prior, J. E. Van Velzen, J. D. Schuijf, F. R. De Graaf, M. A. De Graaf, M. J. Schalij, L. J. Kroft, A. De Roos, J. W. Jukema, E. E. Van Der Wall, J. J. Bax, E. Lankinen, A. Saraste, T. Noponen, R. Klen, M. Teras, T. Kokki, S. Kajander, M. Pietila, H. Ukkonen, J. Knuuti, A. P. Pazhenkottil, R. N. Nkoulou, J. R. Ghadri, B. A. Herzog, R. R. Buechel, S. M. Kuest, M. Wolfrum, O. Gaemperli, L. Husmann, P. A. Kaufmann, D. Andreini, G. Pontone, S. Mushtaq, L. Antonioli, E. Bertella, A. Formenti, S. Cortinovis, G. Ballerini, C. Fiorentini, M. Pepi, A. S. Koh, J. S. Flores, F. Y. J. Keng, R. S. Tan, T. S. J. Chua, A. D. Annoni, G. Tamborini, M. Fusari, A. L. Bartorelli, S. H. Ewe, A. C. T. Ng, V. Delgado, J. Schuijf, F. Van Der Kley, A. Colli, A. De Weger, N. A. Marsan, K. H. Yiu, A. C. Ng, S. A. J. Timmer, P. Knaapen, T. Germans, P. A. Dijkmans, M. Lubberink, J. M. Ten Berg, F. J. Ten Cate, I. K. Russel, A. A. Lammertsma, A. C. Van Rossum, Y. Y. Wong, G. Ruiter, P. Raijmakers, W. J. Van Der Laarse, N. Westerhof, A. Vonk-Noordegraaf, G. Youssef, E. Leung, G. Wisenberg, C. Marriot, K. Williams, J. Etele, R. A. Dekemp, J. Dasilva, D. Birnie, R. S. B. Beanlands, R. C. Thompson, A. H. Allam, L. S. Wann, A. H. Nureldin, G. Adelmaksoub, I. Badr, M. L. Sutherland, J. D. Sutherland, M. I. Miyamoto, G. S. Thomas, H. J. Harms, S. De Haan, M. C. Huisman, R. C. Schuit, A. D. Windhorst, C. Allaart, A. J. Einstein, T. Khawaja, C. Greer, A. Chokshi, M. Jones, K. Schaefle, K. Bhatia, D. Shimbo, P. C. Schulze, A. Srivastava, R. Chettiar, J. Moody, C. Weyman, D. Natale, W. Bruni, Y. Liu, E. Ficaro, A. J. Sinusas, A. Peix, E. Batista, L. O. Cabrera, K. Padron, L. Rodriguez, B. Sainz, V. Mendoza, R. Carrillo, Y. Fernandez, E. Mena, A. Naum, T. Bach-Gansmo, N. Kleven-Madsen, M. Biermann, B. Johnsen, J. Aase Husby, S. Rotevatn, J. E. Nordrehaug, J. Schaap, R. M. Kauling, M. C. Post, B. J. W. M. Rensing, J. F. Verzijlbergen, J. Sanchez, G. Giamouzis, N. Tziolas, P. Georgoulias, G. Karayannis, A. Chamaidi, N. Zavos, K. Koutrakis, G. Sitafidis, J. Skoularigis, F. Triposkiadis, S. Radovanovic, A. Djokovic, D. V. Simic, M. Krotin, A. Savic-Radojevic, M. Pljesa-Ercegovac, M. Zdravkovic, J. Saponjski, S. Jelic, T. Simic, R. Eckardt, B. J. Kjeldsen, L. I. Andersen, T. Haghfelt, P. Grupe, A. Johansen, B. Hesse, H. Pena, G. Cantinho, M. Wilk, Y. Srour, F. Godinho, N. Zafrir, A. Gutstein, I. Mats, A. Battler, A. Solodky, E. Sari, N. Singh, A. Vara, A. M. Peters, A. De Belder, S. Nair, N. Ryan, R. James, S. Dizdarevic, G. Depuey, M. Friedman, R. Wray, R. Old, H. Babla, B. Chuanyong, J. Maddahi, E. Tragardh Johansson, K. Sjostrand, L. Edenbrandt, S. Aguade-Bruix, G. Cuberas-Borros, M. N. Pizzi, M. Sabate-Fernandez, G. De Leon, D. Garcia-Dorado, J. Castell-Conesa, J. Candell-Riera, D. Casset-Senon, M. Edjlali-Goujon, D. Alison, A. Delhommais, P. Cosnay, C. S. Low, A. Notghi, J. 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Kostina, H. Hommel, G. Feuchtner, O. Pachinger, G. Friedrich, A. M. Stel, J. W. Deckers, V. Gama, A. Ciarka, L. A. Neefjes, N. R. Mollet, E. J. Sijbrands, J. Wilczek, C. Llibre Pallares, O. Abdul-Jawad Altisent, H. Cuellar Calabria, P. Mahia Casado, M. T. Gonzalez-Alujas, A. Evangelista Masip, D. Garcia-Dorado Garcia, Y. Tekabe, X. Shen, Q. Li, J. Luma, D. Weisenberger, A. M. Schmidt, R. Haubner, L. Johnson, L. Sleiman, S. Thorn, M. Hasu, M. Thabet, J. N. Dasilva, S. C. Whitman, D. Genovesi, A. Giorgetti, A. Gimelli, G. Cannizzaro, F. Bertagna, G. Fagioli, M. Rossi, R. Bonini, P. Marzullo, C. A. Paterson, S. A. Smith, A. D. Small, N. E. R. Goodfield, W. Martin, S. Nekolla, H. Sherif, S. Reder, M. Yu, A. Kusch, D. Li, J. Zou, M. S. Lloyd, K. Cao, D. W. Motherwell, A. Rice, G. M. Mccurrach, S. M. Cobbe, M. C. Petrie, I. Al Younis, E. Van Der Wall, T. Mirza, M. Raza, H. Hashemizadeh, L. Santos, B. A. Krishna, F. Perna, M. Lago, M. Leo, G. Pelargonio, G. Bencardino, M. L. Narducci, M. Casella, F. Bellocci, S. Kirac, O. Yaylali, M. Serteser, T. Yaylali, A. Okizaki, Y. Urano, M. Nakayama, S. Ishitoya, J. Sato, Y. Ishikawa, M. Sakaguchi, N. Nakagami, T. Aburano, S. V. Solav, R. Bhandari, S. Burrell, S. Dorbala, I. Bruno, C. Caldarella, A. Collarino, M. V. Mattoli, A. Stefanelli, A. Cannarile, F. Maggi, V. Soukhov, S. Bondarev, A. Yalfimov, M. Khan, P. P. Priyadharshan, G. Chandok, T. Aziz, M. Avison, R. A. Smith, D. S. Bulugahapitya, T. Vakhtangadze, F. Todua, M. Baramia, G. Antelava, N.- C. Roche, P. Paule, S. Kerebel, J.- M. Gil, L. Fourcade, A. Tzonevska, K. Tzvetkov, M. Atanasova, V. Parvanova, A. Chakarova, E. Piperkova, B. Kocabas, H. Muderrisoglu, C. P. Allaart, E. Entok, S. Simsek, B. Akcay, I. Ak, E. Vardareli, M. Stachura, P. J. Kwasiborski, G. J. Horszczaruk, E. Komar, A. Cwetsch, B. Zraik, R. Morales Demori, A. D. J. Almeida, M. E. Siqueira, E. Vieira, I. Balogh, G. Kerecsen, E. Marosi, Z. S. Szelid, A. Sattar, T. Swadia, J. Chattahi, W. Qureshi, F. Khalid, A. Gonzalez, S. Hechavarria, K. Takamura, S. Fujimoto, R. Nakanishi, S. Yamashina, A. Namiki, J. Yamazaki, K. Koshino, Y. Hashikawa, N. Teramoto, M. Hikake, S. Ishikane, T. Ikeda, H. Iida, Y. Takahashi, N. Oriuchi, H. Higashino, K. Endo, T. Mochizuki, K. Murase, A. Baali, R. Moreno, M. Chau, H. Rousseau, F. Nicoud, P. Dolliner, L. Brammen, G. Steurer, T. Traub-Weidinger, P. Ubl, P. Schaffarich, G. Dobrozemsky, A. Staudenherz, M. Ozgen Kiratli, B. Temelli, N. B. Kanat, T. Aksoy, G. A. Slavich, G. Piccoli, M. Puppato, S. Grillone, D. Gasparini, S. Perruchoud, C. Poitry-Yamate, M. Lepore, R. Gruetter, T. Pedrazzini, D. Anselm, A. Anselm, H. Atkins, J. Renaud, R. Dekemp, I. Burwash, A. Guo, R. Beanlands, C. Glover, I. Vilardi, B. Zangheri, L. Calabrese, P. Romano, A. Bruno, O. C. Fernandez Cimadevilla, V. A. Uusitalo, M. Luotolahti, M. Wendelin-Saarenhovi, J. Sundell, O. Raitakari, S. Huidu, R. Gadiraju, M. Ghesani, Q. Uddin, B. Wosnitzer, N. Takahashi, E. Alhaj, A. Legasto, B. Abiri, K. Elsaban, T. El Khouly, T. El Kammash, A. Al Ghamdi, B. Kyung Deok, K. Bon Seung, Y. Sang Geun, D. Chang Min, and M. Gwan Hong

Subjects
Cardiology and Cardiovascular Medicine
Published
2011
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6. Morphological variation in crucian brains, with special reference to the origin of the goldfish

Author

Y. Sato, Y. Ojima, K. Takatsuji, and H. Masai

Subjects
Genetics, Morphological variation, Zoology, Animal Science and Zoology, Biology, Molecular Biology, Ecology, Evolution, Behavior and Systematics
Abstract

Summary In fourteen individuals belonging to four Japanese crucian subspecies and one Chinese subspecies, the brain features were studied macroscopically and histologically, in comparison with those of goldfishes domesticated from the crucian. The Chinese crucians, differed from the Japanese crucians, showed brain patterns similar to those of the goldfish, in the relative sizes of the vagal lobe and its sensory layer. The origin of the goldfish was discussed on the basis of brain morphology and other data already presented. Zusammenfassung Morphologische Variation der Gehirne der Karausche, unter besonderer Berucksichtigung des Ursprungs des Goldfisches Von vierzehn Individuen, die zu vier japanischen Unterarten von Karauschen und Chinesischer Karausche gehoren, wurden die Gehirne makroskopisch und histologisch untersucht und mit den Gehirnen von Goldfischen verglichen. Zum Unterschied zu den Japanischen Karauschen zeigten die Chinesischen Karauschen Gehirneigenarten, die denjenigen der Goldfische ahnlich sind; dies gilt insbesondere fur die Grose vom Lobus vagalis und seiner sensorischen Schicht. Der Ursprung des Goldfisches wurde auf Grund der Morphologie des Gehirns und anderer Daten diskutiert.

Published
2009
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7. Comparative morphology of the telencephalon of the Japanese colubrid snakes under consideration of habit

Author

Y. Sato, K. Takatsuji, and H. Masai

Subjects
Morphology (linguistics), biology, Cerebrum, Achalinus, Anatomy, biology.organism_classification, Intermediate type, Dorsal cortex, Natrix, medicine.anatomical_structure, Genetics, medicine, Habit (biology), Animal Science and Zoology, Elaphe, Molecular Biology, Ecology, Evolution, Behavior and Systematics
Abstract

Summary The cytoarchitecture of telencephalon in the colubrid snakes, which are ranged in Japan Island, were studied comparatively under consideration of habit and zoogeographical pattern. The cytoarchitecture of telencephalon is modified in parallel to deviativeness of the ecological character. The dorsal ventricular ridge, the dorsal cortex and nucleus sphaericus are greatly developed in Natrix, Elaphe and Dinodon rufozonatus, while they are reduced in Achalinus. These telencephalic components in Dinodon orientals shows an intermediate type between Natrix-Elaphe group and Achalinus. Among the Japanese colubrid snakes, Achalinus is the most deviate species in both the ecology and telencephalon morphology. Zusammenfassung Vergleichende Untersuchungen uber das Telencephalon japanischer colubrider Schlangen unter Berucksichtigung des Lebensraumes Unter Berucksichtigung der Lebensweise und der zoogeographischen Verbreitung wurde die Zyto-architektur des Endhirns bei den auf den Japan-Inseln verbreiteten colubriden Schlangen untersucht. Die Zytoarchitektur des Endhirns ist, den Unterschieden des okologischen Charakters entsprechend, modifiziert. “Dorsal ventricular ridge”, dorsaler Kortex und Nucleus sphaericus sind bei Natrix, Elaphe und Dinodon rufozonatus starker entwickelt, bei Achalinus reduziert. Diese Bestandteile des Endhirns zeigen bei Dinodon orientals einen intermediaren Typus zwischen der Natrix-Elaphe-Gmppe und Achalinus. Unter den japanischen colubriden Schlangen ist Achalinus die am starksten abweichende Art in Okologie und Morphologie des Endhirns.

Published
2009
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8. The cerebral hemisphere patterns in hybrids produced by artificial crosses of a new species, Bufo torrenticola with Bufo bufo japonicus

Author

K. Takatsuji, Masafumi Matsui, and H. Masai

Subjects
biology, Ecology, Genetics, Bufo bufo japonicus, Zoology, Animal Science and Zoology, biology.organism_classification, Molecular Biology, Bufo torrenticola, Ecology, Evolution, Behavior and Systematics, Hybrid
Abstract

Summary In Japanese toads, B. torrenticola, B. japonicus and their hybrids the cerebral hemisphere patterns were quantitatively studied. The posterior portion of pallium is less developed in B. torrenticola as a relict-like species than in B. japonicus as a advanced toad. The developmental degrees of the posterior portion of pallium in hybrids are intermediate between that of parental species, and furthermore, tend towards maternal side. Zusammenfassung Muster der Groshirnhemispharen von Hybriden, die durch kunstliche Kreuzungen zwischen Bufo torrenticola, einer neuen Art, und Bufo bufo japonicus erzeugt wurden Bei japanischen Kroten, B. torrenticola, B. japonicus und ihren Hybriden wurden die Muster der Groshirnhemispharen quantitativ studiert. Der hintere Abschnitt des Palliums von B. torrenticola, als reliktahnlicher Art, ist weniger entwickelt, im Vergleich zu B. japonicus, einer hoher evoluierten Art. Die Enrwicklungsgrade des hinteren Abschnitts des Palliums sind bei den Hybriden intermediar zwischen den elterlichen Arten, sie neigen zur mutterlichen Seite.

Published
2009
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9. Morphological variability of the brains under domestication from the crucian carp to the goldfish

Author

Y. Sato, K. Takatsuji, and H. Masai

Subjects
Dorsum, biology, Anatomy, biology.organism_classification, Molecular biology, Lobus, Feeding behavior, Relative Volume, Genetics, Crucian carp, Animal Science and Zoology, Brain weight, Molecular Biology, Ecology, Evolution, Behavior and Systematics
Abstract

Summary In connection with domestication, a comparative study was performed on the brain patterns of 99 goldfishes and 52 crucian carps. The brain weight is more decreased in the goldfish than in the crucian carp. Among the major brain subdivisions, the relative size on dorsal aspect and the weight of the vagal lobes are prominently decreased in the goldfish. Within the vagal lobe the relative volume of the sensory layer is reduced in the goldfish, compared with that in the crucian. We suggest that in the microevolutionary process through domestication of the crucian carp towards the goldfish, modification of the brain has occurred in the vagal lobe, as one of the primary gustatory centers much related to feeding behavior. Zusammenfassung Domestikationsveranderungen des Gehirns von der Karausche zum Goldfisch Um Domestikationsveranderungen im Aufbau des Gehirns von der Karausche zum Goldfisch zu erfas-sen, wurden 52 Karauschen und 99 Goldfische untersucht. Das relative Hirngewicht vermindert sich von der Karausche zum Goldfisch. Bereits in der Dorsalansicht ist eine Abnahme der relativen Grose des Lobus vagus auffallig, was sich im Gewicht bestatigt. Im Vergleich zur Karausche ist beim Goldfisch das relative Volumen der sensiblen Schicht gemindert. Im Laufe der Mikroevolution, welcher die Domestikation der Karausche zum Goldfisch' gleich-kommt, ist nach unserer Meinung der Veranderung im Lobus vagus des Gehirns als eine Abnahme eines primaren, fur die Nahrungsaufnahme wichtigen Geschmackszentrums zu deuten.

Published
2009
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10. Roles of phi X174 type primosome- and G4 type primase-dependent primings in initiation of lagging and leading strand syntheses of DNA replication

Author

H. Masai, N. Nomura, Y. Kubota, and Ken-ichi Arai

Subjects
Genetics, Replication Initiation, Bacteriophage phi X174, Cell Biology, Primase, Biology, Origin of replication, Molecular Biology, Biochemistry, dnaC, Primosome, dnaB helicase, Conserved sequence
Abstract

Eleven single strand initiation sequences (ssi) were isolated from various plasmid genomes using a plaque-morphology assay. Out of seven ssi that require dnaB and dnaC functions for replication in a crude in vitro system, six use a phi X174 type priming mechanism, and a phi X174 type primosome is assembled at these sequences from the purified proteins, n'(priA), n(priB), n"(priC), dnaT, dnaB, dnaC, and primase. The same ssi potentiate dATPase activity of n' protein, and thus represent new n' protein recognition sequences (n'-pas). Based on sequence homology, two structural groups are evident. Two sequences show a strong homology with the phi X174 site, whereas three share extensive homology with the previously characterized n'-pas of ColE1, ssiA(ColE1). All the n'-pas have a potential to form stem and loop structures, although sequence homology between the two classes is absent. In addition to the phi X174 type priming, three ssi do not require either dnaB or dnaC function for replication, and use a G4 type priming, requiring only SSB and primase. The 5' ends of primer RNA synthesized by primase are localized within the vicinity of one of the three blocks of highly conserved nucleotide sequences. Deletions of parts of these conserved sequences result in loss of priming activity, suggesting that they are important for priming on the G4 type ssi, which are termed G site. The general significance of these two types of priming in initiation of lagging or leading strand synthesis as well as various modes of initiation at origins of replication are proposed.

Published
1990
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11. Human Cdc7-related kinase complex. In vitro phosphorylation of MCM by concerted actions of Cdks and Cdc7 and that of a criticial threonine residue of Cdc7 bY Cdks

Author

H, Masai, E, Matsui, Z, You, Y, Ishimi, K, Tamai, and K, Arai

Subjects
Threonine, Humans, Nuclear Proteins, Cell Cycle Proteins, Minichromosome Maintenance Complex Component 2, Phosphorylation, Protein Serine-Threonine Kinases, Antigens, Viral, Tumor, Cyclin-Dependent Kinases
Abstract

huCdc7 encodes a catalytic subunit for Saccharomyces cerevisae Cdc7-related kinase complex of human. ASK, whose expression is cell cycle-regulated, binds and activates huCdc7 kinase in a cell cycle-dependent manner (Kumagai, H., Sato, N., Yamada, M., Mahony, D. , Seghezzi, W., Lees, E., Arai, K., and Masai, H. (1999) Mol. Cell. Biol. 19, 5083-5095). We have expressed huCdc7 complexed with ASK regulatory subunit using the insect cell expression system. To facilitate purification of the kinase complex, glutathione S-transferase (GST) was fused to huCdc7 and GST-huCdc7-ASK complex was purified. GST-huCdc7 protein is inert as a kinase on its own, and phosphorylation absolutely depends on the presence of the ASK subunit. It autophosphorylates both subunits in vitro and phosphorylates a number of replication proteins to different extents. Among them, MCM2 protein, either in a free form or in a MCM2-4-6-7 complex, serves as an excellent substrate for huCdc7-ASK kinase complex in vitro. MCM4 and MCM6 are also phosphorylated by huCdc7 albeit to less extent. MCM2 and -4 in the MCM2-4-6-7 complex are phosphorylated by Cdks as well, and prior phosphorylation of the MCM2-4-6-7 complex by Cdks facilitates phosphorylation of MCM2 by huCdc7, suggesting collaboration between Cdks and Cdc7 in phosphorylation of MCM for initiation of S phase. huCdc7 and ASK proteins can also be phosphorylated by Cdks in vitro. Among four possible Cdk phosphorylation sites of huCdc7, replacement of Thr-376, corresponding to the activating threonine of Cdk, with alanine (T376A mutant) dramatically reduces kinase activity, indicative of kinase activation by phosphorylation of this residue. In vitro, Cdk2-Cyclin E, Cdk2-Cyclin A, and Cdc2-Cyclin B, but not Cdk4-Cyclin D1, phosphorylates the Thr-376 residue of huCdc7, suggesting possible regulation of huCdc7 by Cdks.

Published
2000

12. A Cdc7p-Dbf4p protein kinase activity is conserved from yeast to humans

Author

L H, Johnston, H, Masai, and A, Sugino

Subjects
Enzyme Activation, Fungal Proteins, Eukaryotic Cells, Saccharomyces cerevisiae Proteins, Yeasts, Cell Cycle, Humans, Cell Cycle Proteins, Protein Serine-Threonine Kinases, Substrate Specificity
Abstract

DBF4 and CDC7 were identified as budding yeast cell cycle mutants that arrest immediately before S phase. The Dbf4p and Cdc7p proteins interact to form a protein kinase, Cdc7p being the catalytic subunit and Dbf4p is a cyclin-like molecule that activates the kinase in late G1. Dbf4p also targets Cdc7p to origins of replication where likely substrates include the Mcm proteins. Dbf4p and Cdc7p related proteins occur in the fission yeast and in metazoans. These also phosphorylate Mcm proteins and preliminary evidence indicates a similar function to Dbf4p/Cdc7p in budding yeast. The Dbf4p/Cdc7p activity will therefore very likely be conserved in all eukaryotes.

Published
2000

13. Growth regulation of the expression of mouse cDNA and gene encoding a serine/threonine kinase related to Saccharomyces cerevisiae CDC7 essential for G1/S transition. Structure, chromosomal localization, and expression of mouse gene for s. cerevisiae Cdc7-related kinase

Author

J M, Kim, N, Sato, M, Yamada, K, Arai, and H, Masai

Subjects
DNA, Complementary, Saccharomyces cerevisiae Proteins, Base Sequence, Sequence Homology, Amino Acid, Transcription, Genetic, Molecular Sequence Data, G1 Phase, Chromosome Mapping, Cell Cycle Proteins, Exons, Saccharomyces cerevisiae, Sequence Analysis, DNA, Protein Serine-Threonine Kinases, Xenopus Proteins, Introns, S Phase, Mice, Gene Expression Regulation, Animals, Amino Acid Sequence, Cloning, Molecular, Promoter Regions, Genetic, Interphase, In Situ Hybridization, Fluorescence
Abstract

Saccharomyces cerevisiae CDC7 encodes a serine/threonine kinase required for G1/S transition of the yeast cells. We previously reported human and Xenopus cDNAs encoding CDC7-related kinases and suggested the possibility that chromosomal replication of higher eukaryotes may be regulated through conserved mechanisms involving Cdc7-related kinases. Here we report a murine cDNA and gene (muCdc7) encoding a serine/threonine kinase related to CDC7. The predicted coding frame for the longest cDNA for muCdc7 consists of 564 amino acids, which shares 46, 77, and 93% identity, respectively, with those of budding yeast, Xenopus, and human in kinase conserved domains. The chromosomal gene for muCdc7, located at the band 5E5 on the mouse chromosome 5, consists of 12 exons, and its exon/intron organization shares some similarity with that of other protein kinases including Cdk and cAMP-dependent kinase. Transcription of muCdc7, initiated at multiple sites over the 370-base pair promoter region, is repressed in the resting state and is induced at the G1/S boundary after growth factor stimulation in a growth factor-dependent cell line. Transient transfection assays indicated that a 231-base pair segment of the muCdc7 promoter containing three putative E2F binding sites and one Sp1 site but lacking TATA sequence is sufficient for response to growth stimulation.

Published
1998

15. [Two cases of systemic lupus erythematosus presenting with disc edema]

Author

H, Masai and S, Kashii

Subjects
Adult, Pseudotumor Cerebri, Humans, Lupus Erythematosus, Systemic, Female, Papilledema
Abstract

We report two young women, 22 and 19 years old, who showed bilateral optic disc edema in the course of systemic lupus erythematosus. Lumbar puncture showed increased intracranial hypertension with no abnormal findings in the composition of the cerebrospinal fluid. Computed tomography and magnetic resonance imaging showed no abnormal findings. They were diagnosed as having rare intracranial hypertension associated with systemic lupus erythematosus. Treatment with systemic corticosteroids produced a dramatic resolution of the increased intracranial hypertension and the disc edema.

Published
1996

16. Fabrication and Photoluminescent Property of Transparent Nanocrystallized-Glass in Li2O-ZnO-GeO2System

Author

R Ihara, H Masai, Yoshihiro Takahashi, Takumi Fujiwara, Masataka Ando, and Kenichiro Iwasaki

Subjects
Fabrication, Photoluminescence, Materials science, Mineralogy, law.invention, Germanium compounds, Chemical engineering, Photon emission, law, visual_art, visual_art.visual_art_medium, Ceramic, Crystallization, Luminescence, Ternary operation
Abstract

We investigated crystallization behaviour of several glasses in ternary Li2O-ZnO-GeO2 system in order to fabricate the glass-ceramics consisting of Zn-related compound showing photoluminescent property. Nanocrystallized glass with willemite-type Zn2GeO4 could be prepared from the 15Li2O-15ZnO-70GeO2 glass, and revealed a long-lasting photoluminescence (LLP). Origin of the LLP in the nanocrystallized glass is also discussed.

Published
2011
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18. Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome

Author

K Arai and H Masai

Subjects
Genetics, Terminator (genetics), Operon, Base pair, DNA replication, Cell Biology, Biology, Molecular Biology, Biochemistry, Primosome, Gene, dnaC, dnaB helicase
Abstract

dnaT and dnaC of Escherichia coli, whose products are components of the primosome, encode genes essential for both normal chromosomal replication and stable DNA replication induced by SOS signals. dnaT and dnaC together with an unknown gene (P-18), which encodes an 18-kDa polypeptide present downstream of dnaC, are cotranscribed from a promoter located 104 base pairs upstream of dnaT. A portion of the transcripts are processed 16 or 27 base pairs upstream of the dnaC coding region and 20% of the transcripts extending through dnaC are terminated at a terminator 103 base pairs downstream of dnaC. The rest of the transcripts pass through this terminator and are terminated downstream of the P-18 gene. The predicted amino acid sequence of the P-18 protein indicate the presence of a 26-amino acid signal peptide at its N terminus, suggesting that this protein may be secreted into the membrane or a periplasmic space. Another gene, which encodes a 14-kDa hydrophobic protein (P-14), was identified upstream of dnaT. The P-14 gene is transcribed from an upstream promoter, and its transcript extends through dnaT and dnaC. These results indicate that dnaT and dnaC constitute an operon with two unknown genes whose products may be associated with a membrane.

Published
1988
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19. Escherichia coli dnaT gene function is required for pBR322 plasmid replication but not for R1 plasmid replication

Author

H Masai and K Arai

Subjects
DNA Replication, DNA, Bacterial, R1 plasmid, viruses, In Vitro Techniques, Regulatory Sequences, Nucleic Acid, Biology, medicine.disease_cause, Microbiology, Gene product, Plasmid, Escherichia coli, medicine, Molecular Biology, Gene, Plasmid preparation, Temperature, DNA replication, Molecular biology, PBR322, Genes, Bacterial, Mutation, bacteria, Research Article, Plasmids
Abstract

Plasmid pBR322 was unable to replicate in a temperature-sensitive dnaT1 strain at a nonpermissive temperature, whereas a pBR322-derived plasmid carrying the wild-type dnaT+ gene was able to replicate under the same conditions. In contrast to pBR322, plasmid R1 could replicate in the dnaT1 strain at a nonpermissive temperature. In keeping with this finding, in vitro replication of plasmid R1 did not require DnaT protein.

Published
1989
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20. Initiation of lagging-strand synthesis for pBR322 plasmid DNA replication in vitro is dependent on primosomal protein i encoded by dnaT

Author

K Arai and H Masai

Subjects
Genetics, biology, Mutant, Cell Biology, biology.organism_classification, medicine.disease_cause, Biochemistry, Enterobacteriaceae, Primosome, In vitro, PBR322, Cell biology, chemistry.chemical_compound, Plasmid, chemistry, medicine, Molecular Biology, Escherichia coli, DNA
Abstract

The role of the primosome assembly and protein n' recognition site in replication of pBR322 plasmid was examined. The following evidence indicates that the primosome is involved in lagging-strand synthesis of pBR322 plasmid replication in vitro. Early replicative intermediates with newly synthesized leading strand, approximately 1 kilobase pair long, immediately downstream of the replication origin accumulate in products synthesized in extracts from a dnaT strain that lacks primosomal protein i or in wild-type extracts supplemented with anti-protein i antibody. These intermediates are converted efficiently into full-length DNA by addition of purified protein i. Consistent with the previously proposed role of the primosome (Arai, K. and Kornberg, A. (1981) Proc. Natl. Acad. Sci. U. S. A. 78, 69-73), an n' site on the lagging strand, but not on the leading strand, is required for efficient replication of the plasmid in vitro. Plasmids lacking an n' site on the lagging strand replicate only to a limited extent in vitro and early replicative intermediates carrying nascent leading strands are accumulated, although a portion of the intermediates complete replication to yield full-length DNA. The latter reaction is completely inhibited by addition of anti-protein i antibody. Insertion of the n' site of phage phi X174 into pBR322 plasmids lacking lagging-strand n' sites restores the replicative ability of the mutant plasmid comparable to that of the wild-type plasmid. These results indicate that protein i is essential for lagging-strand synthesis of pBR322 plasmid in vitro and that it may play an important role in the priming events as a part of either an n' site-dependent primosome or an n' site-independent, as yet unidentified, priming complex.

Published
1988
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21. Leading strand synthesis of R1 plasmid replication in vitro is primed by primase alone at a specific site downstream of oriR

Author

K.-I. Arai and H. Masai

Subjects
DnaG, Sense strand, Replication Initiation, Prokaryotic DNA replication, Coding strand, DNA replication, Cell Biology, Primase, Biology, Molecular Biology, Biochemistry, Molecular biology, DnaA
Abstract

By using an in vitro system for R1 plasmid replication dependent on a plasmid-encoded repA protein and host dnaA protein, 5' ends of the nascent leading strand were located at positions 1986-1992, some 380 base pair downstream of oriR. Analyses of early replication intermediates generated in vitro in the presence of dideoxy TTP also indicated that replication initiates about 400 base pair downstream of oriR and proceeds unidirectionally. When a 418-base single-stranded DNA from position 1778 to 2195, derived from the leading strand template, was cloned onto an M13 vector, the chimeric single-stranded phage could be replicated in vitro with only single-stranded DNA binding protein, primase (dnaG gene product), and DNA polymerase III holoenzyme. Furthermore, the priming occurred at a site identical to leading strand initiation. These results strongly suggest that the leading strand synthesis is primed by primase alone. The lagging strand synthesis is specifically terminated at position 1515 or 1516 within oriR, preventing further leftward fork movement. Based on these results, a scheme of R1 plasmid replication is presented.

Published
1989
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22. Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast.

Author

T, Takeda, K, Ogino, K, Tatebayashi, H, Ikeda, Ki, Arai, and H, Masai

Abstract

Hsk1, Saccharomyces cerevisiae Cdc7-related kinase in Shizosaccharomyces pombe, is required for G1/S transition and its kinase activity is controlled by the regulatory subunit Dfp1/Him1. Analyses of a newly isolated temperature-sensitive mutant, hsk1-89, reveal that Hsk1 plays crucial roles in DNA replication checkpoint signaling and maintenance of proper chromatin structures during mitotic S phase through regulating the functions of Rad3 (ATM)-Cds1 and Rad21 (cohesin), respectively, in addition to expected essential roles for initiation of mitotic DNA replication through phosphorylating Cdc19 (Mcm2). Checkpoint defect in hsk1-89 is indicated by accumulation of cut cells at 30 degrees C. hsk1-89 displays synthetic lethality in combination with rad3 deletion, indicating that survival of hsk1-89 depends on Rad3-dependent checkpoint pathway. Cds1 kinase activation, which normally occurs in response to early S phase arrest by nucleotide deprivation, is largely impaired in hsk1-89. Furthermore, Cds1-dependent hyperphosphorylation of Dfp1 in response to hydroxyurea arrest is eliminated in hsk1-89, suggesting that sufficient activation of Hsk1-Dfp1 kinase is required for S phase entry and replication checkpoint signaling. hsk1-89 displays apparent defect in mitosis at 37 degrees C leading to accumulation of cells with near 2C DNA content and with aberrant nuclear structures. These phenotypes are similar to those of rad21-K1 and are significantly enhanced in a hsk1-89 rad21-K1 double mutant. Consistent with essential roles of Rad21 as a component for the cohesin complex, sister chromatid cohesion is partially impaired in hsk1-89, suggesting a possibility that infrequent origin firing of the mutant may affect the cohesin functions during S phase.

Published
2001

23. Co-porphyrin functionalized CVD graphene ammonia sensor with high selectivity to disturbing gases: hydrogen and humidity.

Author

K. Sawada, T. Tanaka, T. Yokoyama, R. Yamachi, Y. Oka, Y. Chiba, H. Masai, J. Terao, and K. Uchida

Abstract

Recently low-power, small gas sensors have been strongly demanded to realize “super smart society.” In particular, ammonia: NH3 sensors are expected to be key devices for breath diagnosis. However, it is difficult for NH3 sensors to obtain high selectivity against hydrogen: H2, since conventional metal-oxide gas sensors respond to any reducing gases. In this study, Co-porphyrin functionalized graphene sensors were fabricated, and selective NH3 sensing was realized by the selective metal-ligand bond of Co with NH3. The sensor successfully detected sub-ppm NH3, while it showed no response to high concentration H2. Furthermore, we investigated the effect of humidity on the Co-porphyrin functionalized graphene NH3 sensor. We demonstrated the detection of low concentration NH3 even under rapid humidity changes. We considered that the key sensing mechanism of our sensor is charge transfer to graphene by the electronic structure change of Co-porphyrin-graphene complexes due to NH3 adsorption on Co-porphyrin. [ABSTRACT FROM AUTHOR]

Published
2020
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24. Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome

Author

H, Masai and K, Arai

Subjects
DNA Replication, Base Sequence, Transcription, Genetic, Molecular Sequence Data, Nucleotide Mapping, DNA Restriction Enzymes, Chromosomes, Bacterial, Blotting, Northern, Bacterial Proteins, Genes, Genes, Bacterial, Operon, Escherichia coli, Amino Acid Sequence, Plasmids
Abstract

dnaT and dnaC of Escherichia coli, whose products are components of the primosome, encode genes essential for both normal chromosomal replication and stable DNA replication induced by SOS signals. dnaT and dnaC together with an unknown gene (P-18), which encodes an 18-kDa polypeptide present downstream of dnaC, are cotranscribed from a promoter located 104 base pairs upstream of dnaT. A portion of the transcripts are processed 16 or 27 base pairs upstream of the dnaC coding region and 20% of the transcripts extending through dnaC are terminated at a terminator 103 base pairs downstream of dnaC. The rest of the transcripts pass through this terminator and are terminated downstream of the P-18 gene. The predicted amino acid sequence of the P-18 protein indicate the presence of a 26-amino acid signal peptide at its N terminus, suggesting that this protein may be secreted into the membrane or a periplasmic space. Another gene, which encodes a 14-kDa hydrophobic protein (P-14), was identified upstream of dnaT. The P-14 gene is transcribed from an upstream promoter, and its transcript extends through dnaT and dnaC. These results indicate that dnaT and dnaC constitute an operon with two unknown genes whose products may be associated with a membrane.

Published
1988

27. Brain organization of sharks, with special reference to archaic species

Author

Y, Sato, K, Takatsuji, and H, Masai

Subjects
Cerebral Cortex, Species Specificity, Cerebellum, Sharks, Animals, Brain, Globus Pallidus, Biological Evolution
Abstract

Using photographic measurements and normal neurohistological preparations, a number of brains of Galeomorph and Squalomorph sharks were studied and compared. The brains are generally more developed in Galeomorph than in Squalomorph sharks. Among the major brain territories in Galeomorph sharks, the nucleus centralis is prominently enlarged, and the corpus cerebelli is much swollen and fissured. In archaic species such as Scapanorhynchus, Heterodontus, and Chlamydoselachus, the cerebral pallium and corpus cerebelli are less developed than those of taxonomically close species. These results were discussed, in relation to taxonomy, phylogeny and ecology.

Published
1983

32. Leading strand synthesis of R1 plasmid replication in vitro is primed by primase alone at a specific site downstream of oriR

Author

H, Masai and K, Arai

Subjects
DNA Replication, Base Sequence, Single-Strand Specific DNA and RNA Endonucleases, DNA, Single-Stranded, Nucleic Acid Hybridization, RNA Nucleotidyltransferases, DNA Primase, DNA Restriction Enzymes, Templates, Genetic, Endonucleases, DNA-Binding Proteins, Bacterial Proteins, DNA, Viral, Escherichia coli, Thymine Nucleotides, Bacteriophages, Cloning, Molecular, Bacteriophage phi X 174, DNA Polymerase III, Dideoxynucleotides, Plasmids
Abstract

By using an in vitro system for R1 plasmid replication dependent on a plasmid-encoded repA protein and host dnaA protein, 5' ends of the nascent leading strand were located at positions 1986-1992, some 380 base pair downstream of oriR. Analyses of early replication intermediates generated in vitro in the presence of dideoxy TTP also indicated that replication initiates about 400 base pair downstream of oriR and proceeds unidirectionally. When a 418-base single-stranded DNA from position 1778 to 2195, derived from the leading strand template, was cloned onto an M13 vector, the chimeric single-stranded phage could be replicated in vitro with only single-stranded DNA binding protein, primase (dnaG gene product), and DNA polymerase III holoenzyme. Furthermore, the priming occurred at a site identical to leading strand initiation. These results strongly suggest that the leading strand synthesis is primed by primase alone. The lagging strand synthesis is specifically terminated at position 1515 or 1516 within oriR, preventing further leftward fork movement. Based on these results, a scheme of R1 plasmid replication is presented.

Published
1989

33. Initiation of lagging-strand synthesis for pBR322 plasmid DNA replication in vitro is dependent on primosomal protein i encoded by dnaT

Author

H, Masai and K, Arai

Subjects
DNA Replication, Kinetics, Bacterial Proteins, Genes, Genes, Bacterial, Immune Sera, Mutation, Escherichia coli, Plasmids
Abstract

The role of the primosome assembly and protein n' recognition site in replication of pBR322 plasmid was examined. The following evidence indicates that the primosome is involved in lagging-strand synthesis of pBR322 plasmid replication in vitro. Early replicative intermediates with newly synthesized leading strand, approximately 1 kilobase pair long, immediately downstream of the replication origin accumulate in products synthesized in extracts from a dnaT strain that lacks primosomal protein i or in wild-type extracts supplemented with anti-protein i antibody. These intermediates are converted efficiently into full-length DNA by addition of purified protein i. Consistent with the previously proposed role of the primosome (Arai, K. and Kornberg, A. (1981) Proc. Natl. Acad. Sci. U. S. A. 78, 69-73), an n' site on the lagging strand, but not on the leading strand, is required for efficient replication of the plasmid in vitro. Plasmids lacking an n' site on the lagging strand replicate only to a limited extent in vitro and early replicative intermediates carrying nascent leading strands are accumulated, although a portion of the intermediates complete replication to yield full-length DNA. The latter reaction is completely inhibited by addition of anti-protein i antibody. Insertion of the n' site of phage phi X174 into pBR322 plasmids lacking lagging-strand n' sites restores the replicative ability of the mutant plasmid comparable to that of the wild-type plasmid. These results indicate that protein i is essential for lagging-strand synthesis of pBR322 plasmid in vitro and that it may play an important role in the priming events as a part of either an n' site-dependent primosome or an n' site-independent, as yet unidentified, priming complex.

Published
1988

35. New heat-stable acid-phosphatase test for differentiation of mycobacteria

Author

H Saito and H Masai

Subjects
Microbiology (medical), Microbiological Techniques, biology, Chemistry, Acid phosphatase test, Acid Phosphatase, Acid phosphatase, biology.organism_classification, Microbiology, Mycobacterium, Biochemistry, Reagent, biology.protein, Research Article
Abstract

A simple, rapid, heat-stable (70 degrees C) acid-phosphatase test for differentiation of mycobacteria was developed with the alpha-naphthylphosphoric acid-diazonium o-dianisidine color reagent.

Published
1980

36. A new heat-stable acid phosphatase test for mycobacteria

Author

H, Saito, K, Yamaoka, K, Kiyotani, and H, Masai

Subjects
Diagnosis, Differential, Heating, Bacteriological Techniques, Mycobacterium Infections, Acid Phosphatase, Animals, Humans, Mycobacterium
Abstract

The heat-stable (70degrees C) acid phosphatase test performed by the method of Kind and King is a simple method for differentiating Mycobacterium kansasii, M. marinum, M. gastri, M. nonchromogenicum, and M. triviale from other slowly growing mycobacteria, and M. fortuitum from other rapidly growing acid-fast bacilli.

Published
1976

38. Comparative Studies on the Distribution of Monoamine Oxidase and Succinic Dehydrogenase in Vertebrates’ Forebrain

Author

T. Kusunoki, H. Masai, and H. Ishibashi

Subjects
Succinic dehydrogenase, medicine.anatomical_structure, Biochemistry, Monoamine oxidase, Forebrain, Central nervous system, medicine, Thalamic nucleus, Neural tube, Biology, Sulcus limitans, Neuroscience, psychological phenomena and processes
Abstract

The purpose of this work is to study the chemoarchitectonics of the forebrain, related to a fundamental plan of the central nervous system of vertebrates. In the architectural organization of the central nervous system of vertebrates the sulcus limitans of His is the most important landmark which runs rostrocaudally to the lateral walls of the neural tube in the embryonal stage.

Published
1966
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41. [Fundamental structure of the brain stem of vertebrates]

Author

H, Masai

Subjects
Amphibians, Birds, Mammals, Succinate Dehydrogenase, Sensory Receptor Cells, Fishes, Animals, Humans, Paleontology, Reptiles, Alkaline Phosphatase, Monoamine Oxidase, Brain Stem
Published
1967

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