1. Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
- Author
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S.F.S. Domingues, H.S. Ferreira, J.A.P.C. Muniz, A.K.F. Lima, O.M. Ohashi, J.R. Figueiredo, and L.D.M. Silva
- Subjects
monkey ,preantral follicle ,isolation ,ovary ,Cebus apella ,Animal culture ,SF1-1100 - Abstract
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.
- Published
- 2003
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