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ABSTRACT From 1913 onwards, the global situation changed from a scenario of nitrogen (N) scarcity to an abundance of ammonia (NH3) produced synthetically via the Haber-Bosch process. Several N compounds have been synthesized since then, with urea becoming the main source of N, accounting for 55 % of current N consumption. However, N efficiency in agroecosystems is low and, normally, N recovery in cultivated plants is less than 50 %. This occurs because a large amount of reactive N is lost to the environment, inducing various forms of pollution, threatening human and environmental health, in addition to causing a negative economic impact on the farmer. The main processes responsible for low N efficiency are NH3 volatilization, leaching, and N denitrification. Considering global NH3 volatilization losses of 14 %, it can be assumed that up to 8.6 million Mg of urea are lost every year in the form of NH3. For each ton of NH3 produced, 1.9 to 3.8 Mg of CO2 is emitted into the atmosphere. Therefore, increasing N use efficiency (NUE) without compromising yield is a necessity and a challenge for crop improvement programs and current management systems, in addition to reducing greenhouse gas emissions. In this context, enhanced efficiency fertilizers (EEFs), which contain technologies that minimize the potential for nutrient losses compared to conventional sources, are an alternative to increasing the efficiency of nitrogen fertilization. Currently, EEFs are classified into three categories: stabilized, slow-release, and controlled-release. This study aims to understand the technologies used to produce EEFs and the factors that govern their availability to plants. This review covers the following topics: the discovery of N, N dynamics in the soil-atmosphere system, N assimilation in plants, strategies to increase NUE in agrosystems, NH3 synthesis, NH3 volatilization losses, N fertilizer technologies, the importance of characterization of EEFs, conventional nitrate or ammonium-based fertilizers to reduce gaseous losses of NH3 and future prospects for the use of N fertilizers in agriculture.

Abstract This Opinion highlights several successful cases of biofuel technologies recently described by the IEA Bioenergy Intertask Report on Lessons Learned. The report discussed the potential of biofuels to contribute to a significant market supply, thus replacing fossil fuels and mitigating global warming, and it underscores the challenges in expanding biofuel production and replicating successful models between countries and regions. Based on the lessons learned from conventional, established technologies, the authors analyzed policies, feedstocks, products, technologies, economics, environmental concerns, social aspects, scalability, and ease of implementation and replication in different countries or regions. There are blending mandates in place around the world to foster the use of biofuels. Dependence on the availability and price fluctuations of crop feedstocks may limit biofuel production in certain circumstances. Legal restrictions on using food crops as feedstocks present obstacles to scaling up production. Temporary constraints related to feedstock costs and availability, as evidenced by changes and postponements of biofuel blending mandates in various countries (particularly during the COVID‐19 pandemic) also pose challenges. Technological hurdles exist for advanced biofuels that implicate premium pricing. Still, 2G ethanol from sugarcane meets very strict feedstock requirements with a carbon footprint so low that only electric vehicles charged in Norway could have life‐cycle GHG emissions at the same level as a 2G ethanol‐fueled combustion engine car. The authors evaluate whether and how much electrification could contribute to advance the decarbonization efforts in different countries. Drawing from these observations, the authors express their viewpoints to assist researchers and policymakers in the energy sector in formulating viable approaches to combat the climate crisis.

Micronutrient fertilization is usually neglected by producers, especially for peanut, a crop that is frequently grown in crop rotation systems due to its low perceived nutrient requirements. New peanut cultivars are able to achieve high yields when grown under suitable conditions. However, fertilization recommendation tables are dated and do not consider the need for micronutrients. To support improvements in these recommendations, this study quantified the micronutrient demand (B, Cu, Fe, Mn, and Zn) of three runner peanut cultivars (IAC Runner 886, IAC 505, and IAC OL3) during the biological cycle and the transport of these micronutrients to pods and kernels. The experiment was carried out in a randomized complete block with a split-plot design and nine replications. The whole plots consisted of the three peanut cultivars, and the subplots comprised nine plant samplings (at 14, 28, 42, 56, 70, 84, 105, 126, and 147 days after emergence (DAE)). These modern peanut cultivars exhibited high uptake and accumulation of Fe, but the proportion of Fe removed by pods and kernels was lowest among all analyzed micronutrients. The second-most-accumulated micronutrient was Mn. The maximum requirement for micronutrients of peanut occurred around 84 DAE, and IAC 505 had the highest micronutrient uptake and accumulation among the cultivars (especially at later stages), followed by IAC OL3 and IAC Runner 886. Our results provide new insights into micronutrient requirements for peanut and demonstrate the need for new fertilizer recommendation programs for peanut cultivation.

Soil parent material is a fundamental natural resource for the generation of new soils. Through weathering processes, soil parent materials provide many of the basic building blocks for soils and have a significant bearing on the physico-chemical makeup of the soil profile. Parent materials are critical for governing the stock, quality, and functionality of the soil they form. Most research on soil parent materials to date has aimed to establish and measure the processes by which soil is generated from them. Comparatively little work has been performed to assess the rates at which soil parent materials erode if they are exposed at the land surface. This is despite the threat that the erosion of soil parent materials poses to the process of soil formation and the loss of the essential ecosystem services those soils would have provided. A salient but unanswered question is whether the erosion of soil parent materials, when exposed at the land surface, outpaces the rates at which soils form from them. This study represents one of the first to detect and measure the loss of soil parent material. We applied Uncrewed Aerial Vehicle Structure-From-Motion (UAV-SfM) photogrammetry to detect, map, and quantify the erosion rates of an exposed saprolitic (i.e., weathered bedrock) surface on an agricultural hillslope in Brazil. We then utilized a global inventory of soil formation to compare these erosion rates with the rates at which soils form in equivalent lithologies and climatic contexts. We found that the measured saprolite erosion rates were between 14 and 3766 times faster than those of soil formation in similar climatic and lithological conditions. While these findings demonstrate that saprolite erosion can inhibit soil formation, our observations of above-ground vegetation on the exposed saprolitic surface suggests that weathered bedrock has the potential to sustain some biomass production even in the absence of traditional soils. This opens up a new avenue of enquiry within soil science: to what extent can saprolite and, by extension, soil parent materials deliver soil ecosystem services?

Inputs of carbon (C) and nutrients from organic residues may select specific microbes and shape the soil microbial community. However, little is known about the abiotic filtering of the same residues with different nutrient concentrations applied to the soil. In our study, we explored how applying organic residue, vinasse, as fertilizer in its natural state (V) versus its concentrated form (CV) impacts soil microbiota. We conducted two field experiments, evaluating soil prokaryotic and fungal communities over 24 and 45 days with vinasse (V or CV) plus N fertilizer. We used 16S rRNA gene and ITS amplicon sequencing. Inorganic N had no significant impact on bacterial and fungal diversity compared to the control. However, the varying concentrations of organic C and nutrients in vinasse significantly influenced the soil microbiome structure, with smaller effects observed for V compared to CV. Prokaryotic and fungal communities were not correlated (co-inertia: RV coefficient = 0.1517, p = 0.9708). Vinasse did not change the total bacterial but increased the total fungal abundance. A higher C input enhanced the prokaryotic but reduced the fungal diversity. Our findings highlight vinasse’s role as an abiotic filter shaping soil microbial communities, with distinct effects on prokaryotic and fungal communities. Vinasse primarily selects fast-growing microorganisms, shedding light on the intricate dynamics between organic residues, nutrient concentrations, and soil microbes.

Foliar fertilization has been used as a supplemental strategy to plant nutrition especially in crops with high yield potential. Applying nutrients in small doses stimulates photosynthesis and increases yield performance. The aim of this study was to evaluate the efficiency of foliar application of molybdenum (Mo) to soybean and maize. The treatments consisted of the presence (+Mo) and absence (-Mo) of supplementation. Plant nutritional status, nitrate reductase (NR) activity, gas exchange parameters, photosynthetic enzyme activity (Rubisco in soybean and maize and PEPcase in maize), total soluble sugar concentration, leaf protein content, shoot dry matter, shoot nitrogen accumulated, number of grains per plant, mass of 100 grains, and grain yield were evaluated. For soybean and maize, application of Mo increased leaf NR activity, nitrogen and protein content, Rubisco activity, net photosynthesis, and grain yield. These results indicate that foliar fertilization with Mo can efficiently enhance nitrogen metabolism and the plant’s response to carbon fixation, resulting in improved crop yields.

Liming contributes to the alleviation of acidity in highly weathered soils. For sugarcane, the use of green harvest methods and new soil tillage systems requires an adjustment of lime application rates. In the present study, the effects of different lime rates and tillage systems on sugarcane performance and soil chemical fertility parameters were assessed. Three experiments were conducted in two locations between April 2015 and October 2019. The study design was a randomized block field study with four replicates. Four lime rates were applied once at sugarcane establishments in each soil tillage system and location: no liming (control); lime recommended rate (LRR); two times LRR (2× LRR); and three times LRR (3× LRR). The three soil tillage systems were conventional (CT), deep-strip (DT), and modified deep-strip tillage (MDT). Soil chemical fertility, leaf nutrient concentrations, and sugarcane yield components were analyzed, and correlations were identified by principal component analysis (PCA). The soil acidity was adequately alleviated in all tillage systems. Increasing the lime rate improved the lime distribution and soil fertility parameters. Applying lime at rates higher than LRR improved stalk and sugar yields, longevity, agronomic efficiency index (AEI), and correlated with a longer residual effect of liming, mainly in the last ratoon.

Intercropping grain with forage crops bridges the gap between agriculture and sustainability. In tropical regions, forage grasses are increasingly being adopted as winter pasture intercropped and in rotation with maize to maximize food production. However, current recommendations for nitrogen (N) fertilizer application are based on monocropped maize (Zea mays), and the best N management approach for intercropping systems remains unclear. A field experiment was carried out in three growing seasons with three intercropping systems [monoculture maize, intercropped with palisadegrass (Urochloa brizantha), and intercropped with guineagrass (Megathyrus maximus)] combined with six different split applications of N to maize (0–0, 100–0, 70–30, 50–50, 30–70, and 0–100 kg N ha−1 at seeding-sidedressing) with four replicates. We measured dry matter (DM) and accumulated N in maize and forage grasses, as well as maize production components and yields. Additionally, land equivalent ratio, relative crowding coefficient, aggressivity of maize with forage grasses, forage crude protein (CP) concentration, estimated animal stocking rate, and estimated meat production and economic outcomes. Greatest maize yield was 8.7 Mg ha−1 for monocropped maize. However, favorable maize yield was also obtained in intercropping systems. Although no difference was observed between intercropping systems, applying all N at sidedressing of maize negatively affected maize and forage yields and, consequently, land use and economic evaluation. For both intercropping systems, estimated meat and land use were 114 and 10% higher when N fertilizer was applied than the control (0–0 kg N ha−1), on average. Maize-forage grass intercropping is a viable alternative production system for improving yields and land use. In addition, estimated meat production and revenue can be enhanced with palisadegrass or guineagrass. At least half of the N fertilizer must be applied early in the growing season of maize to maximize production of the entire system.

Nitrogen management in crops is a key activity for agricultural production. Methods that can determine the levels of this element in plants in a quick and non-invasive way are extremely important for improving production systems. Within several fronts of study on this subject, proximal and remote sensing methods are promising techniques. In this regard, this research sought to demonstrate the relationships between variations in leaf nitrogen content (LNC) and sugarcane spectral behaviour. The work was carried out in three experimental areas in São Paulo State, Brazil, with different soils, varieties and nitrogen rates during the 2012/13 and 2013/14 seasons. A significant correlation was observed between the LNC and variations in the sugarcane spectra. The green and red-edge spectral bands were the most consistent and stable predictors of LNC among the evaluated harvests. Stepwise multiple linear regression analysis (MSLR) generated better models for LNC estimation when calibrated with experimental area, independent of the variety. The present research demonstrates that specific wavelengths are associated with the variation in LNC in sugarcane, and these are reported in the green region (near 550 nm) and in the red-edge wavelengths (680 to 720 nm). These results may help in future research on the direct in situ application of nitrogen fertilizers.

Intercropping forage grasses with upland rice is an alternative cropping system to improve agroecosystem diversification and could potentially enhance sustainability in tropical regions. However, nitrogen (N) immobilization and nutrient competition between rice and forage grasses could reduce rice grain yield and decrease overall productivity. Therefore, fertilizer N requirements of upland rice intercropped with forage grasses needs to be better defined. Field experiments were carried out during three growing seasons on a Typic Haplorthox soil in São Paulo state of Brazil. The experimental design was a randomized block design with a 3 × 4 factorial scheme with four replications. Treatments were cropping system [monocropped rice (Oryza sativa L.), rice intercropped with palisadegrass (Urochloa brizantha), and rice intercropped with guineagrass (Megathyrsus maximus) and sidedress N application rate (0, 40, 80, and 120 kg N ha−1). Intercropped grasses were sown between upland rice rows 30 days after rice emergence. On average, intercropping of rice with palisadegrass or guineagrass decreased rice shoot dry matter and grain yield by 11% and milled rice productivity by 10% compared with monocropped rice. Grain yield, grain protein, and milled productivity of rice increased as N application rate increased. Forage dry matter production (first and second cut) and crude protein (second cut) were greatest in the rice + palisadegrass intercropping system. Production of both forage grasses increased with up to 80 kg N ha−1 in the first cut and increased linearly with N in the second cut. Intercropping of rice with palisadegrass or guineagrass with 80 kg N ha−1 application resulted in the greatest land equivalent ratio (1.96 and 1.55, respectively). Relative N yield was greatest at 120 kg N ha−1 (220 and 173%, respectively). Although rice monocropping had greatest grain yield, intercropping systems with forage grasses were more favorable from both economic and environmental perspectives by enhancing plant diversification, nutrient cycling with forage grasses, land use production per unit area, and profitability throughout the year.

Abstract Enhanced efficiency fertilizers (EEF) are of interest for sugarcane (Saccharum spp.) production due to the potential to reduce N losses, and improve crop yield and environmental conditions. This study was conducted to determine the effect of urea with N‐(n‐butyl) thiophosphoric triamide (NBPT) or dicyandiamide (DCD) on NH3 volatilization and sugarcane yield. The NH3 volatilization, the foliar concentration of N and S, and yield of stalk and pol were monitored in two field trials testing six N sources (urea; urea + NBPT; urea + DCD; ammonium sulfate [AS]; ammonium nitrate [AN]; ammonium sulfate nitrate), and three N rates (50, 100, and 150 kg ha−1). The N losses from urea totaled 22 and 18% in Sites 1 and 2, respectively. Treating urea with NBPT reduced NH3 volatilization by 60%. Sugarcane yield increased 8.8; 11.6; and 16.0 Mg ha−1 (Site 1), and 4.5, 9.3, and 14.2 Mg ha−1 (Site 2) with the application of 50, 100, and 150 kg ha−1 N as compared to control, respectively. All N sources increased yields, demonstrating similar efficiency for sugarcane production. Green harvesting sugarcane cultivated in sandy soils with low organic matter concentration is highly responsive to N, showing similar efficiency between EEF and conventional N fertilizers.

Urea is the most widely used nitrogen (N) fertilizer, with a projected increase in annual demand of 1.5% in the coming years. After its application to soil, urea undergoes hydrolysis via the urease enzyme, causing increases in the soil pH in the surrounding area of the granules and resulting in NH3 losses that average 16% of N applied worldwide and can reach 40% or more in hot and humid conditions. The use of urease inhibitors is an effective way to reduce NH3 losses. Several compounds act as urease inhibitors, but only N-(n-butyl) thiophosphoric triamide (NBPT) has been used worldwide, being the most successful in a market that has grown 16% per year in the past 10 years. Only in the past three years other compounds are being commercially launched. In comparison to urea, NBPT-treated urea reduces NH3 loss by around 53%. Yield gain by NBPT usage is of the order of 6.0% and varies from −0.8 to 10.2% depending on crop species. Nitrification inhibitors usually increase NH3 volatilization and mixing them with urease inhibitors partially offsets the benefits of the latter in reducing NH3 loss. The efficacy of NBPT to reduce NH3 loss is well documented, but there is a need for further improvement to increase the period of inhibition and the shelf life of NBPT-treated urea. Keywords: NBPT, NPPT, Ammonia volatilization, Soil urease, Nutrient use efficiency, Urea, Nitrogen fertilizer

Abstract Background The production of 1 L of ethanol from sugarcane generates up to 12 L of vinasse, which is a liquid waste containing an as-yet uncharacterized microbial assemblage. Most vinasse is destined for use as a fertilizer on the sugarcane fields because of the high organic and K content; however, increased N2O emissions have been observed when vinasse is co-applied with inorganic N fertilizers. Here we aimed to characterize the microbial assemblage of vinasse to determine the gene potential of vinasse microbes for contributing to negative environmental effects during fertirrigation and/or to the obstruction of bioethanol fermentation. Results We measured chemical characteristics and extracted total DNA from six vinasse batches taken over 1.5 years from a bioethanol and sugar mill in Sao Paulo State. The vinasse microbial assemblage was characterized by low alpha diversity with 5–15 species across the six vinasses. The core genus was Lactobacillus. The top six represented bacterial genera across the samples were Lactobacillus, Megasphaera and Mitsuokella (Phylum Firmicutes, 35–97% of sample reads); Arcobacter and Alcaligenes (Phylum Proteobacteria, 0–40%); Dysgonomonas (Phylum Bacteroidetes, 0–53%); and Bifidobacterium (Phylum Actinobacteria, 0–18%). Potential genes for denitrification but not nitrification were identified in the vinasse metagenomes, with putative nirK and nosZ genes the most represented. Binning resulted in 38 large bins with between 36.0 and 99.3% completeness, and five small mobile element bins. Of the large bins, 53% could be classified at the phylum level as Firmicutes, 15% as Proteobacteria, 13% as unknown phyla, 13% as Bacteroidetes and 6% as Actinobacteria. The large bins spanned a range of potential denitrifiers; moreover, the genetic repertoires of all the large bins included the presence of genes involved in acetate, CO2, ethanol, H2O2, and lactose metabolism; for many of the large bins, genes related to the metabolism of mannitol, xylose, butyric acid, cellulose, sucrose, “3-hydroxy” fatty acids and antibiotic resistance were present based on the annotations. In total, 21 vinasse bacterial draft genomes were submitted to the genome repository. Conclusions Identification of the gene repertoires of vinasse bacteria and assemblages supported the idea that organic carbon and nitrogen present in vinasse together with microbiological variation of vinasse might lead to varying patterns of N2O emissions during fertirrigation. Furthermore, we uncovered draft genomes of novel strains of known bioethanol contaminants, as well as draft genomes unknown at the phylum level. This study will aid efforts to improve bioethanol production efficiency and sugarcane agriculture sustainability.

The productive potential of new peanut cultivars has increased over the years in relation to old cultivars, especially when compared with ones with upright growth habit. Thus, the requirement for macronutrients for these new cultivars may also have increased, making the existing fertilizer recommendation tables obsolete, thus increasing the need for further studies measuring the real macronutrient requirements of these new peanut cultivars. Our study aimed to evaluate the growth patterns and the macronutrient absorption rate throughout the biological cycle of three modern runner peanut cultivars, as well as the potential for producing dry matter, pods, and kernels, and their respective macronutrient accumulations. The experimental design was a randomized complete block with split-plots and nine replications. The experimental plots consisted of three peanut cultivars (IAC Runner 886, IAC 505, and IAC OL3), and subplots consisted of nine plant samplings (14, 28, 42, 56, 70, 84, 105, 126, and 147 days after emergence (DAE)). Our results showed that modern peanut cultivars presented nutrient accumulation around 30 to 40 days earlier than older cultivars, as well as increasing the uptake by K and Ca. IAC 505 absorbed higher amounts of macronutrients and resulted in greater dry matter production compared with IAC OL3 and IAC Runner 886. Our study demonstrated that the most appropriate time for plants to find greater availability of nutrients in the soil is 70 to 84 DAE, in addition to highlighting the need for updates on nutritional recommendations for higher yields of modern peanut cultivars.

O objetivo do trabalho foi avaliar o efeito das plantas de cobertura e da fonte de nitrogênio nas bactérias nitrificantes e amonificantes do solo, bem como nos teores de nitrato e amônio. O experimento foi conduzido em um latossolo vermelho distrófico sobre sistema plantio direto (SPD) por seis anos. O delineamento experimental foi em blocos casualizados, no esquema de parcelas subdivididas, com quatro repetições. As parcelas foram constituídas por seis espécies de plantas de cobertura do solo (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria ruziziensis, Pennisetum americanum e Crotalaria spectabilis) e as subparcelas pelo controle e três fontes de nitrogênio (1- controle, sem aplicação de N, 2- nitrato de cálcio, 3- sulfato de amônio e 4- sulfato de amônio + dicianodiamida (DCD)) aplicadas imediatamente após a emergência do arroz na dose de 40 kg ha-1 de N. Foram avaliadas a atividade das bactérias e os teores de nitrato e amônio no solo aos 15 DAE. As plantas de cobertura milheto (Pennisetum americanum), crotalaria (Crotalaria spectabilis); Brachiaria brizantha, B. decumbens; e B. humidicola proporcionaram os maiores teores de amônio no solo; O uso do inibidor de nitrificação (dicianodiamida-DCD) inibiu parte das bactérias nitrificantes e proporcionou os maiores teores de amônio no solo; A atividade das bactérias amonificantes e nitrificantes foi maior nas parcelas cultivadas com braquiárias; Plantas de cobertura aliada ao uso de DCD pode ser uma estratégia para aumentar os teores de amônio do solo cultivado sobre SPD

ABSTRACT The use of nitrification inhibitors (NIs; dicyandiamide - DCD) is an alternative to reduce oxidation of ammonium (NH4+-N) to nitrate (NO3–-N) in the soil, reducing NO3–-N losses from fertilization practices. Based on the hypothesis that DCD reduces conversion of NH4+-N to NO3–-N in tropical soils and inhibits ammonia oxidizing microorganisms (AOM) abundance, soils from the Piracicaba region, São Paulo, with different textures (sand, loam and clay) were incubated with ammonium sulphate (AS) and DCD. Contents of NH4+-N, NO3–-N, soil pH, and AOM abundance were quantified periodically. Ammonium sulphate increased AOM abundance in all soils, but AS+DCD presented AOM abundances similar to the control. During 90 days of incubation, the effectiveness of DCD in reducing NO3–-N production was 1.8, 86.4, and 145.6 mg kg–1, while the effectiveness of DCD in reducing AOM abundance was 1.2, 3.0 and 2.3 × 10–3 g soil–1 for sandy, loamy, and clayey soils, respectively. DCD effectiveness was greater in loamy and clayey soils due to the naturally low nitrification in sandy soils. Application of AS treated with DCD showed potential not only to reduce NO3–-N production in loamy and clayey soils, but also to decrease the soil nitrification rate. Overall, DCD was effective in reducing AOM abundance and conversion of NH4+-N to NO3–-N in loamy and clay soils evaluated here. The increase in clay content directly influences DCD effectiveness in reducing conversion of NH4+-N to NO3–-N.

Bioenergy is an important and feasible option for mitigating global warming and climate change. However, large-scale land-use change (LUC) to expand bioenergy crops, such as sugarcane, raises concerns about the potential negative environmental and socioeconomic side effects. Such effects are context-specific, and depending on the LUC scenario and management practices, several co-benefits can be attained. We reviewed the literature and discussed how LUC and best management practices affect key components of sustainability (e.g., soil health, soil carbon (C) sequestration, greenhouse gas emissions (GHG) emissions, nutrient cycling, water quality, among others) of sugarcane-derived bioenergy production in Brazil. Sugarcane expansion has occurred predominantly over pasture areas, although converting croplands could be also an environmentally feasible option. The land transition from low-productivity pastures to sugarcane cultivation seems to be a sustainable pathway to increase bioenergy production. This LUC scenario enhances soil health and soil C sequestration over time, although soil compaction, biodiversity loss, and erosion are still challenging. Besides, adopting best management practices, such as conservation tillage, sustainable crop residue management, rational fertilization, and recycling by-products, has been fundamental to ensuring sustainable bioenergy production. Public policies and well-designed legal frameworks and regulations, such as the Forest Code and the RenovaBio legislations in Brazil, are necessary to make bioenergy production compatible with rational land use and protection. Lastly, our analysis provided insights into sugarcane expansion over a small proportion (1%) of pasture areas in Latin American and Caribbean (LAC) and sub-Saharan African (SSA) countries, which may result in a substantial impact on global bioenergy supply. We concluded that sugarcane-derived bioenergy is a sustainable option to tackle climate change while provisioning other key ecosystem services and promoting socioeconomic development.

ABSTRACT Retaining the mineral N in the form of NH4+ in the soil for a lengthy period is desirable for reducing losses. Furthermore, there is evidence that sugarcane prefers NH4+-N in place of NO3−-N. This study aimed firstly, to evaluate the potential of root extracts of Bracchiaria humidicola andSaccharum spontaneum, in contrast with the DCD (Dicyandiamide) inhibitor, to increase absorption of N by plants fertilized with ammonium sulfate, and secondly, to quantify the emission of N2O fluxes with the use of this inhibitor. The experiment was developed in a glasshouse in an entirely randomized design where four treatments were applied: AS) ammonium sulfate (control); AS+DCD) ammonium sulfate associated with dicyandiamide; AS+BCH) ammonium sulfate associated with root extracts ofBrachiaria humidicola; and AS+SCS) ammonium sulfate associated with root extracts of Saccharum spontaneum. Differences were observed in biomass production in plants 45 and 60 days after fertilization (DAF) and 15 and 60 days in biomass accumulation of roots. The application of AS associated with DCD synthetic inhibitor kept NO3−-N values low throughout the evaluation period, while in other treatments the concentration increased right up to the second evaluation 15 DAF. Sugarcane plants did not benefit from the increased presence of ammoniacal N promoted by DCD. The use of DCD reduced the average flux of N2O during the evaluation period compared to plants receiving AS treatments only, which was not observed when root extracts of B. humidicola and S. spontaneum were used.

Organic vinasse, a residue produced during bioethanol production, increases nitrous oxide (N2O) emissions when applied with inorganic nitrogen (N) fertilizer in soil. The present study investigated the role of the ammonia-oxidizing bacteria (AOB) community on the N2O emissions in soils amended with organic vinasse (CV: concentrated and V: non-concentrated) plus inorganic N fertilizer. Soil samples and N2O emissions were evaluated at 11, 19, and 45 days after fertilizer application, and the bacterial and archaea gene (amoA) encoding the ammonia monooxygenase enzyme, bacterial denitrifier (nirK, nirS, and nosZ) genes and total bacteria were quantified by real time PCR. We also employed a deep amoA amplicon sequencing approach to evaluate the effect of treatment on the community structure and diversity of the soil AOB community. Both vinasse types applied with inorganic N application increased the total N2O emissions and the abundance of AOB. Nitrosospira sp. was the dominant AOB in the soil and was correlated with N2O emissions. However, the diversity and the community structure of AOB did not change with vinasse and inorganic N fertilizer amendment. The results highlight the importance of residues and fertilizer management in sustainable agriculture and can be used as a reference and an input tool to determine good management practices for organic fertilization.

Abstract:The objective of this work was to evaluate whether a canopy sensor is capable of estimating sugarcane response to N, as well as to propose strategies for handling the data generated by this device during the decision-making process for crop N fertilization. Four N rate-response experiments were carried out, with N rates varying from 0 to 240 kg ha-1. Two evaluations with the canopy sensor were performed when the plants reached average stalk height of 0.3 and 0.5 m. Only two experiments showed stalk yield response to N rates. The canopy sensor was able to identify the crop response to different N rates and the relationship of the nutrient with sugarcane yield. The response index values obtained from the canopy sensor readings were useful in assessing sugarcane response to the applied N rate. Canopy reflectance sensors can help to identify areas responsive to N fertilization and, therefore, improve sugarcane fertilizer management.

O cultivo do arroz no ambiente terras altas parece ser, dentre as principais culturas, o menos adaptado ao sistema plantio direto (SPD). Uma das razões atribuídas para esta menor adaptação ao SPD seria a baixa atividade da enzima nitrato redutase (NR), o que dificultaria a assimilação de nitrogênio pelas plântulas. Entretanto, pode haver diferenças entre os genótipos quanto à atividade dessa enzima. Desse modo, o objetivo deste trabalho foi determinar a atividade da enzima NR em distintos cultivares de arroz de terras altas e em diferentes épocas do início de desenvolvimento da cultura. O experimento foi conduzido em condições controladas, em delineamento experimental em blocos completos casualizados com os tratamentos arranjados em esquema fatorial, 10 (cultivares de arroz) x 4 (épocas de avaliações), com quatro repetições. As avaliações da atividade da enzima NR foram realizadas no terço médio do tecido foliar fresco. Houve interação entre cultivares e épocas de avaliação (p=0,0178). A atividade enzimática da NR foi superior na cultivar IAC 202, aos 7 dias após a emergência (DAE). Aos 14 DAE, as cultivares Carajás, IAC 202, BRS Sertaneja, BRS Bonança e BRS Curinga apresentaram elevada atividade desta enzima. Aos 21 DAE, a atividade da NR foi superior nas cultivares Primavera e BRS Bonança. Entretanto, aos 28 DAE, não houve diferenças entre as cultivares quanto a esta variável. Conclui-se que atividade da enzima NR, na cultura do arroz de terras altas, é dependente da cultivar, do período de desenvolvimento vegetal e decresce com o avanço do ciclo da cultura.

The grain yield of upland rice under no-tillage has been unsatisfactory and one reason could be the nitrate/ammonium balance in the soil. Cover crops and nitrogen fertilization can be used to change the nitrate/ammonium relation in the soil and improve conditions for the development of upland rice in the no-tillage (NT) system. The aim was to study the effect of cover crops and nitrogen sources on grain yield of upland rice under no tillage. The study was carried out on the Fazenda Experimental Lageado, in Botucatu, State of São Paulo, Brazil, in an Oxisol area under no-tillage for six years. The experiment was arranged in a randomized block split-plot design with four replications. The plots consisted of six cover crop species (Brachiaria brizantha, B. decumbens, B. humidicola, B. ruziziensis, Pennisetum americanum, and Crotalaria spectabilis) and the split-plots of seven forms of N fertilizer management. Millet is the best cover crop to precede upland rice under NT. The best form of N application, as nitrate, is in split rates or total rate at topdressing or an ammonium source with or without a nitrification inhibitor, in split doses. When the cover crops C. spectabilis, B. brizantha, B. decumbens, B. humidicola, and B. ruziziensis preceded rice, they induced the highest grain yield when rice was fertilized with N as ammonium sulfate source + nitrification inhibitor in split rates or total dose at topdressing.

The use of cover crops can change the soil NO3-: NH4+ ratio, providing larger amounts of NH4+, and enable the development of crops which absorb more or prefer this form of nitrogen (N), like rice. This study aimed at evaluating the effect of the interaction of cover crops and N sources, with and without nitrification inhibitor (dicyandiamide), under no-tillage system (NTS). The experiment was carried out in 2009/2010, in Botucatu, São Paulo State, Brazil, in an Oxisol under NTS for six years. The experimental design was randomized blocks, arranged in a split plot, with four replications. The plots consisted of six cover crops species (Urochloa brizantha, U. decumbens, U. humidicola, U. ruziziensis, Pennisetum americanum and Crotalaria spectabilis) and the split plots were seven forms of nitrogen fertilization, at 0 and 30 days after the emergence (DAE) of rice [1 - control, without N fertilization; 2 - calcium nitrate (40 + 40 kg ha-1); 3 - calcium nitrate (0 + 80 kg ha-1); 4 - ammonium sulfate (40 + 40 kgha-1); 5 - ammonium sulfate (0 + 80 kg ha-1); 6 - ammonium sulfate + dicyandiamide (40 + 40 kg ha-1); and 7 - ammonium sulfate + dicyandiamide (0 + 80 kg ha-1)]. The use of nitrification inhibitor and the C. spectabilis cover crop resulted in higher levels of ammonium in the soil. The application of the ammonium source without nitrification inhibitor in a total dose at 30 DAE and with inhibitor (split or total dose) resulted in the highest levels of nitrate in the soil.

The lowest grain yield of rice under no-tillage system (NTS) in relation to the conventional system may be due to the predominance nitrate in the soil and the low nitrate reductase activity. Another reason may be caused by micronutrient deficiency because of superficially soil acidity corrections. Therefore, the objective of this study was to evaluate the changes caused by soil pH in the N forms in the soil, micronutrients concentration in rice plants, nitrate reductase activity, yield of rice and its components. The experiment was performed in a greenhouse conditions. The experimental design was a completely randomized in a factorial three (levels of soil acidity) x five (micronutrients sources) with four replications. The addition of micronutrients does not affect levels of nitrate and ammonium in the soil; soil acidity significantly affects levels of nitrate and ammonium in the soil, concentration of micronutrients in rice plants and crop yield and its components; medium soil acidity (pH 5.5) result in medium to high levels of Cu and Fe, medium level of Zn and Mn, high nitrate reductase activity, resulting in higher dry matter, tillers, panicles, spikelets, weight of 100 grains and hence grain yield.

The maintenance of sugarcane (Saccharum spp.) straw on a soil surface increases the soil carbon (C) stocks, but at lower rates than expected. This fact is probably associated with the soil management adopted during sugarcane replanting. This study aimed to assess the impact on soil C stocks and the humification index of soil organic matter (SOM) of adopting no-tillage (NT) and conventional tillage (CT) for sugarcane replanting. A greater C content and stock was observed in the NT area, but only in the 0-5 cm soil layer (p < 0.05). Greater soil C stock (0-60 cm) was found in soil under NT, when compared to CT and the baseline. While C stock of 116 Mg ha-1 was found in the baseline area, in areas under CT and NT systems the values ranged from 120 to 127 Mg ha-1. Carbon retention rates of 0.67 and 1.63 Mg C ha-1 year-1 were obtained in areas under CT and NT, respectively. Laser-Induced Fluorescence Spectroscopy showed that CT makes the soil surface (0-20 cm) more homogeneous than the NT system due to the effect of soil disturbance, and that the SOM humification index (H LIF) is larger in CT compared to NT conditions. In contrast, NT had a gradient of increasing H LIF, showing that the entry of labile organic material such as straw is also responsible for the accumulation of C in this system. The maintenance of straw on the soil surface and the adoption of NT during sugarcane planting are strategies that can increase soil C sequestration in the Brazilian sugarcane sector.

The area under mechanized sugarcane (Saccharum spp.) harvesting is expanding in Brazil, increasing the return of trash to the soil. The main questions regarding this management are: (i) after adopting unburned mechanical harvesting, how long will it take to observe decreases in fertilizer requirements, (ii) what will be the magnitude of this decrease and, (iii) the impact in the short run of removing trash for energy purposes in the nutrient cycling? This study aimed to build an N prediction model for long term assessment of the contribution of sugarcane crop residues to sugarcane nutrition and to evaluate the cycling of other nutrients derived from crop residues. Keeping crop residues over the soil will increase soil N stock and N recovery by sugarcane, reaching equilibrium after 40 years with recovery of approximately 40 kg ha-1 year-1 of N. Removing trash for energy production will decrease the potential reduction in N fertilizer requirement. Of the total nutrients in the trash, 75 % of the K2O (81 kg ha-1 year-1) and 50 % of the N (31 kg ha-1 year-1) are in the tops, indicating the importance of maintaining tops in the soil to sustain soil fertility. Because the input data employed in the simulations are representative of the conditions in Southeast Brazil, these results might not be definitive for situations not represented in the experiments used in the study, but the model produced is useful to forecast changes that occur in the soil under different trash management.

O objetivo deste trabalho foi avaliar o acúmulo de matéria seca e de nutrientes de plantas forrageiras consorciadas com o milho safrinha, em função da adubação nitrogenada. As espécies Urochloa brizantha cv. Marandu, U. decumbens cv. Basilisk, U. ruziziensis cv. Comum e Panicum maximum cv. Tanzânia, com semeadura em meados de março, foram avaliadas em quatro municípios paulistas, em consórcio com o milho. Utilizou-se o delineamento experimental de blocos ao acaso, com quatro repetições, em parcelas subdivididas. Nas parcelas, foram avaliados os consórcios e nas subparcelas, quatro doses de nitrogênio em cobertura (0, 30, 60 e 90 kg ha-1). Foi determinado o acúmulo de matéria seca e de nutrientes pelas plantas forrageiras em três épocas: no florescimento, na maturidade fisiológica do milho e por ocasião da dessecação das forrageiras, em outubro. O acúmulo de matéria seca das forrageiras intensifica-se após a colheita do milho safrinha. Quando as forrageiras são semeadas na entrelinha do milho, a adubação nitrogenada em cobertura não interfere no acúmulo de matéria seca e de nutrientes pelas forrageiras, e o consórcio não afeta a produtividade de grãos de milho safrinha.

Legumes as green manure are alternative sources of nitrogen (N) for crops and can supplement or even replace mineral nitrogen fertilization due to their potential for biological nitrogen fixation (BNF). The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulfate (AS) was evaluated using the 15N tracer technique. N was added at the rate of 196 and 70 kg ha-1 as 15Nlabeled sunn hemp green manure (SH) and as ammonium sulfate (AS), respectively. Treatments were: (i) Control; (ii) AS15N; (iii) SH15N + AS; (iv) SH15N; and (v) AS15N + SH. Sugarcane was cultivated for five years and was harvested three times. 15N recovery was evaluated in the two first harvests. In the sum of the three harvests, the highest stalk yields were obtained with a combination of green manure and inorganic N fertilizer; however, in the second cutting the yields were higher where SH was used than in plots with AS. The recovery of N by the first two consecutive harvests accounted for 19 to 21% of the N applied as leguminous green manure and 46 to 49% of the N applied as AS. The amounts of inorganic N, derived from both N sources, present in the 0-0.4 m layer of soil in the first season after N application and were below 1 kg ha-1.Leguminosas, como adubo verde, são fontes alternativas de nitrogênio para as culturas e podem complementar ou mesmo substituir a adubação mineral nitrogenada, devido ao seu potencial de fixação biológica de nitrogênio (FBN). A utilização do nitrogênio pela cana-de-açúcar (Saccharum spp.) fertilizada com crotalária (Crotalaria juncea L.) e sulfato de amônio (SA) foi avaliada utilizando a técnica de traçador 15N. As quantidades equivalentes a 196 e 70 kg de N por hectare foram adicionados como adubo verde crotalária júncea (CJ) e como o sulfato de amônio (SA), respectivamente, nos seguintes tratamentos: (i) controle; (ii) SA15N; (iii) CJ15N + SA; (iv) CJ15N; e (v) SA15N + CJ. A cana-de-açúcar foi cultivada por cinco anos e colhida três vezes. A recuperação do 15N foi avaliada nas duas primeiras colheitas. Na soma das três safras, os maiores rendimentos de colmos foram obtidos com uma combinação de adubos verdes e fertilizantes N inorgânicos, mas, no segundo corte rendimentos superiores foram observados nos tratamentos com CJ em comparação com os observados com SA. A recuperação de N nas primeiras duas safras consecutivas representou 19 a 21% do N aplicado como adubo verde e 46 a 49% do N aplicado como SA. As quantidades de N inorgânico derivado das fontes marcadas, presentes na camada 0-0,4 m do solo na primeira safra após a aplicação do N foram inferiores a 1 kg ha-1.

A field experiment was carried out on an Ultisol located at the city of Agudos (22º30'S; 49º03'W), in the state of São Paulo, Brazil, in order to determine the effects of rates and sources of potassium fertilizer on nutritional status of 'Smooth Cayenne' pineapple and on some soil chemical properties. The experiment was a complete factorial design with four rates (0, 175, 350, and 700 kg ha-1 of K2O) and three combinations of K sources (100% KCl, 100% K2SO4 and 40% K2SO4 + 60% KCl). Soil samples were taken from the depths 0-20 cm, 20-40 cm and 40-60 cm at planting and 14 months after. Nutritional status of pineapple plants was assessed by means of tissue analysis. Soil K availability increased with application of K fertilizer, regardless of K sources. Soil chlorine and Cl concentration in pineapple leaves increased with application of KCl or K2SO4+KCl. Plant uptake of potassium was shaped by soil K availability and by the application rates of K fertilizer, independently of K sources.

A field experiment with pineapple (Smooth Cayenne) was carried out on an Ultisol located in the city of Agudos (22º30'S; 49º03'W), in the state of São Paulo, Brazil, with the objective of investigating the effects of rates and sources of potassium fertilizer on plant growth and fruit yield. The experiment was a complete factorial design (4x3) with four rates (0, 175, 350, and 700 kg ha-1 of K2O) and three combinations of K sources (100% KCl, 100% K2SO4, and 40% K2SO4 + 60% KCl). Plant growth and fruit yield were evaluated. Biomass accumulation of pineapple plants was impaired by chlorine added with potassium chloride. Fruit yield increased with potassium fertilization. At high rates of K application, fertilization with K2SO4 showed better results than with KCl. Detrimental effects of KCl were associated with excess of chlorine.

A cana-de-açúcar (Saccharum spp.) vem sendo cultivada no Brasil para produção de açúcar e agroenergia. Em seu sistema de produção, após um ciclo de 4 a 8 anos, é possível a rotação com plantas de cobertura, antes do seu replantio, para melhoria do solo e geração de renda. Estudou-se a caracterização e produtividade de biomassa de leguminosas (como adubos-verdes) e girassol (Helianthus annuus L.), a ocorrência natural de micorrizas, o teor de açúcar e a produtividade em colmos da cana-de-açúcar IAC 87-3396 e a viabilidade econômica desse sistema com cultivo após as opções de rotação, com quantificação da produtividade durante três cortes consecutivos. O amendoim (Arachis hypogaea L.) cv. IAC-Caiapó, girassol cv. IAC-Uruguai e mucuna-preta (Mucuna aterrimum Piper and Tracy) foram as culturas que apresentaram maior percentagem de colonização por fungos micorrízicos. O girassol foi a planta de cobertura que mais extraiu nutrientes do solo, seguido por amendoim (Arachis hipogaea L.) cv. IAC-Tatu e feijão-mungo (Vigna radiata L. Wilczek). A colonização por fungos micorrízicos mostrou correlação positiva com a altura de plantas de cana no primeiro corte (p = 0,01 e R = 0,52), mas não se correlacionou com a produtividade de colmos ou açúcar. No primeiro corte, o girassol foi a cultura de rotação que ocasionou o maior aumento de produtividade, da ordem de 46% em colmos e de 50% na quantidade de açúcar, em comparação com a testemunha. Com exceção dos amendoins, todas as culturas em rotação aumentaram a renda líquida do sistema na média de três cortes de cana-de-açúcar.Sugarcane (Saccharum spp.) is an important crop for sugar production and agro-energy purposes in Brazil. In the sugarcane production system after a 4- to 8-year cycle crop rotation may be used before replanting sugarcane to improve soil conditions and give an extra income. This study had the objective of characterizing the biomass and the natural colonization of arbuscular mycorrhizal fungi (AMF) of leguminous green manure and sunflower (Helianthus annuus L.) in rotation with sugarcane. Their effect on stalk and sugar yield of sugarcane cv. IAC 87-3396 grown subsequently was also studied. Cane yield was harvested in three subsequent cuttings. Peanut cv. IAC-Caiapó, sunflower cv. IAC-Uruguai and velvet bean (Mucuna aterrimum Piper and Tracy) were the rotational crops that resulted in the greater percentage of AMF. Sunflower was the specie that most extracted nutrients from the soil, followed by peanut cv. IAC-Tatu and mung bean (Vigna radiata L. Wilczek). The colonization with AMF had a positive correlation with sugarcane plant height, at the first cut (p = 0.01 and R = 0.52) but not with the stalk or cane yields. Sunflower was the rotational crop that brought about the greatest yield increase of the subsequent sugarcane crop: 46% increase in stalk yield and 50% in sugar yield compared with the control. Except for both peanut varieties, all rotational crops caused an increase in net income of the cropping system in the average of three sugarcane harvests.

The original version of the article contained a mistake. The accession number has been incorrectly published in the Availability of data and materials section.

Estudos com isótopos estáveis são cada vez mais comuns pela qualidade e confiabilidade dos resultados obtidos e por não apresentarem risco ao homem e ao ambiente. No entanto, é necessário o uso de microparcelas para diminuir o custo do projeto. Por suas áreas serem muito reduzidas em relação à parcela onde está inserida, o objetivo deste trabalho foi comparar o estado nutricional de plantas das microparcelas com as do restante das parcelas experimentais, na fase de máximo desenvolvimento da cultura da cana-de-açúcar, em experimentos com doses de N. Foram instalados três experimentos, no Estado de São Paulo, com três doses de N e uma testemunha, com quatro repetições, em delineamento em blocos completos casualizados. Nas parcelas, de 48 linhas de 15 m cada, foram instaladas microparcelas de 3 m² com fertilizante-15N. Na época de máximo desenvolvimento da cultura foram coletadas folhas-diagnóstico para a avaliação de seu estado nutricional em todas as parcelas e microparcelas. Nessas folhas foram determinados os teores de macronutrientes, e os resultados foram submetidos à análise de variância. Foram comparados, em cada área experimental e de forma conjunta, os resultados das parcelas com os das microparcelas quanto aos teores foliares dos nutrientes e, quando significativo o efeito das doses de N na variação desses, foram feitas regressões. Não houve diferença entre os teores de N, P, Ca, Mg e S das folhas-diagnóstico entre as parcelas e as microparcelas, as quais foram consideradas representativas da área experimental. A adubação nitrogenada aumentou os teores dos nutrientes nas folhas-diagnóstico, o que deve ser considerado na avaliação nutricional da cultura quando este tipo de experimento for realizado.Studies with stable isotopes are becoming more common due to the increased safety of operation and quality and reliability of results. However, the use of microplots is required to decrease the costs of such studies. Since microplots are small compared to regular plot areas, the purpose of this study was to investigate whether nutritional data based on microplot samples can adequately represent the whole area, in a comparison of the nutritional status of microplot sugar cane plants at their maximum development stage with those of the regular plots in experiments with N rates. Three experiments were set up, with three N rates (40, 80, and 120 kg ha-1 N) and a control, with four repetitions, in a randomized complete block design, in the state of São Paulo. Microplots of 3 m² containing 15N-fertilizer (5.04% atom 15N) were included in the main plots formed by 48 lines of sugar cane spaced 1.5 m apart. At the time of maximum development stage, diagnostic leaves were collected in the main and microplots to evaluate the nutritional status of plants by analyzing the total concentration of macronutrients. There were no differences in N, P, Ca, Mg, and S concentrations in the diagnostic leaves from the main and microplots, so that the latter can be considered representative of the experimental area. Higher nitrogen fertilizer rates induced increased concentrations of not only N, but also of P, Ca, Mg, and S in the diagnostic leaves.

Assessing the spatial variability of soil chemical properties has become an important aspect of soil management strategies with a view to higher crop yields with minimal environmental degradation. This study was carried out at the Centro Experimental of the Instituto Agronomico, in Campinas, São Paulo, Brazil. The aim was to characterize the spatial variability of chemical properties of a Rhodic Hapludox on a recently bulldozer-cleaned area after over 30 years of coffee cultivation. Soil samples were collected in a 20 x 20 m grid with 36 sampling points across a 1 ha area in the layers 0.0-0.2 and 0.2-0.4 m to measure the following chemical properties: pH, organic matter, K+, P, Ca2+, Mg2+, potential acidity, NH4-N, and NO3-N. Descriptive statistics were applied to assess the central tendency and dispersion moments. Geostatistical methods were applied to evaluate and to model the spatial variability of variables by calculating semivariograms and kriging interpolation. Spatial dependence patterns defined by spherical model adjusted semivariograms were made for all cited soil properties. Moderate to strong degrees of spatial dependence were found between 31 and 60 m. It was still possible to map soil spatial variability properties in the layers 0-20 cm and 20-40 cm after plant removal with bulldozers.A avaliação da variabilidade espacial dos atributos químicos do solo tem se tornado importante ferramenta na determinação de estratégias de manejo que visam aumentar a produtividade agrícola com menor degradação ambiental. O presente trabalho foi realizado no Centro Experimental Central do Instituto Agronômico, localizado em Campinas/SP, com o objetivo de caracterizar a variabilidade espacial dos atributos químicos de um Latossolo Vermelho após a remoção de um cafezal, cultivado por mais de 30 anos, com trator de esteira. As amostras de solo foram coletadas em grade georreferenciada de 20 x 20 m, totalizando 36 pontos nas camadas de 0-0,2 e 0,2-0,4 m, para determinação dos seguintes atributos químicos: pH, matéria orgânica do solo (MOS), K+, P, Ca2+, Mg2+, acidez potencial (H + Al), N-NH4 e N-NO3. A variabilidade espacial foi estudada utilizando-se a geoestatística, por meio da análise de semivariogramas e da interpolação por krigagem. Para todos os atributos estudados foi possível estabelecer uma estrutura de dependência espacial definida por um semivariograma ajustado a um modelo esférico. Foram encontradas dependências espaciais de moderada a forte, com valores de alcance variando de 31 a 60 m. Mesmo com a remoção das plantas com trator de esteira, foi possível mapear a variabilidade espacial dos atributos do solo nas camadas de 0-0,2 e 0,2-0,4 m de profundidade.

Due to their nitrogen fixation potential, legumes represent an alternative for supplying nutrients, substituting or complementing mineral fertilization in cropping systems involving green manuring. The objective of this study was to evaluate the N balance in a soil-plant system involving green manures [sunn hemp (Crotalaria juncea L.) and velvet bean (Mucuna aterrima Piper & Tracy)], both labeled with 15N. They were incorporated into two soils of contrasting textural classes: a clayey Eutrudox and a sandy-clayey Paleudalf, both cultivated with corn. The research was carried out in a greenhouse, using pots containing 6 kg of air dried soil, to which the equivalent to 13 Mg ha-1 dry matter of above-ground mass plus 2.7 or 2.2 Mg ha-1 of velvet bean and sunn hemp roots were incorporated, respectively, with 15N labeling of either shoots or roots. One hundred days after emergence of the corn, the velvet bean residues provided higher accumulation of N in the soil, higher absorption by corn plants and accumulation in the shoot. The green manure decomposition was more intense in the medium-textured Paleudalf. The highest nitrogen losses were also observed in this soil.Em função de seu potencial de fixação de nitrogênio, as leguminosas representam uma alternativa ao suprimento, substituição ou complementação da adubação mineral em sistemas de cultivos envolvendo adubação verde. O objetivo deste estudo foi avaliar o balanço do N no sistema solo planta com adubos verdes crotalária júncea (Crotalaria juncea L.) e mucuna-preta (Mucuna aterrima Piper & Tracy), marcadas com 15N, incorporadas em dois solos de diferentes classes texturais: Latossolo Vermelho eutroférrico textura argilosa, A moderado (LVef) e Argissolo Vermelho-Amarelo distrófico textura arenosa/média, A moderado (PVAd), e cultivados com milho. O trabalho foi desenvolvido em casa-de-vegetação, em vasos contendo 6 kg de terra aos quais foi incorporado o equivalente a 13 Mg ha-1 de massa seca da parte aérea e 2,7 ou 2,2 Mg ha-1 de raízes de mucuna-preta e de crotalária júncea, respectivamente. A marcação com 15N foi efetuada ou nas raízes ou na parte aérea. Cem dias após emergência do milho, a incorporação de mucuna-preta aos solos proporcionou maior acúmulo de nitrogênio no solo, maior absorção do elemento pelas plantas de milho e sua acumulação na parte aérea. A decomposição da parte aérea e raízes dos adubos verdes foi mais intensa no solo de textura média (PVAd). Neste solo, também, foram observadas as maiores perdas de nitrogênio.

O experimento foi realizado em canavial comercial, com a variedade SP81 3250, na Usina São Martinho (Pradópolis-SP), em Latossolo Vermelho-Escuro de textura argilosa, com o objetivo de avaliar a mineralização da palha de cana-de-açúcar e sua composição após um ciclo de desenvolvimento da cultura. Foi utilizado um delineamento experimental de blocos completos casualizados, com quatro repetições. Sacos de telas que continham palha marcada em 15N (1,07 % de átomos de 15N), em quantidades equivalentes a 9 t ha-1 de matéria seca, foram colocados entre as fileiras de cana-planta, em todos os tratamentos (0, 40, 80 e 120 kg ha-1 de N). Após 14 meses (de junho 2005 a agosto 2006), foram retirados os sacos para a quantificação do material seco remanescente e para determinações de N, de isótopos de 15N e do teor de C, por espectrometria de massas. A decomposição da palhada nos sacos foi maior nos tratamentos adubados com N e o balanço de massa subestimou a liberação do N da palha em comparação com os dados obtidos com a técnica isotópica. Após 14 meses, verificou-se que 37 a 65 % da matéria seca do material da palhada remanescente sobre o solo eram compostos por restos de raízes da cana cultivada durante esse período, pela contaminação por solo e por microrganismos que se desenvolveram na palhada, indicando que os processos ocorridos durante a decomposição da palhada são mais dinâmicos do que os avaliados pelo balanço de massas.The experiment was conducted with the sugar cane variety SP81 3250, in a commercial area of the sugar mill São Martinho (Pradópolis-SP), on a clay-texture Rhodic eutrustox. The experimental had a randomized block design with four replications. Bags containing straw enriched with 15N (1.07 % 15N atoms) equivalent to 9 t ha-1 dry matter were placed on the soil surface between the sugar cane plant rows in all treatments (0, 40, 80 and 120 kg ha-1 of N). After 14 months (from June 2005 to August 2006) the bags were removed to quantify the remaining dry material, determine total N and C, and 15N isotopic concentration by mass spectrometry. Results indicated that straw decomposition was higher in the N fertilized treatments and that the mass balance underestimated the N amounts released from straw. The isotopic technique showed that after 14 months, between 37 and 65 % of the dry mass of the straw remaining on the soil surface came from the live sugar cane rooting system or from microorganisms and soil residues adhered to the straw. This suggests that the processes that occur during straw decomposition are more dynamic than those evaluated by mass-balance equations.

Legal restrictions from burning sugarcane prior to harvest are causing a sharp increase in acreage which is harvested as green cane. The presence of a thick sugarcane trash mulch left after harvest makes it difficult to incorporate fertilisers in the soil. Since large losses of ammonia may occur when urea is surface applied to trash, it is important to find ways to improve urea-N use efficiency. The urease inhibitor NBPT slows down urea hydrolysis and thus may help decrease ammonia losses. Ammonia traps were set up in seven sugarcane fields covered with trash and fertilised with ammonium sulfate or ammonium nitrate, urea, and NBPT-treated urea. All N fertilisers were surface-applied at rates of 80 or 100 kg N ha-1. Very little N was lost when ammonium nitrate or ammonium sulfate were used. However, volatilisation losses as ammonia from the urea treatments varied from 1% (rainy days after fertilisation) to 25% of the applied N. The percentage of reduction in volatilisation due to NBPT application ranged from 15% to 78% depending on the weather conditions during the days following application of N. Addition of NBPT to urea helped to control ammonia losses, but the inhibitor was less effective when rain sufficient to incorporate urea into the soil occurred only 10 to 15 days or latter after fertiliser application.Restrições legais à colheita de cana-de-açúcar com despalha a fogo estão causando um aumento da área cultivada com cana crua. Essa prática gera uma espessa camada de palha de cana sobre o solo após a colheita, o que dificulta a incorporação de fertilizantes. Uma vez que grandes quantidades de amônia podem ser perdidas quando a uréia é aplicada superficialmente sobre a palha, é importante buscar alternativas para maximizar a eficiência de uso do N-uréia. O inibidor de urease NBPT retarda a hidrólise da uréia e pode contribuir para diminuir as perdas de amônia por volatilização. Para quantificar essas perdas, foram instaladas câmaras coletoras de amônia em sete áreas de produção de cana-de-açúcar colhida sem queima; estas foram fertilizadas com sulfato ou nitrato de amônio, uréia ou uréia tratada com NBPT. Todos os fertilizantes nitrogenados foram aplicados superficialmente em doses de 80 ou 100 kg ha-1de N. As perdas de N foram muito pequenas quando se usou nitrato ou sulfato de amônio. Entretanto, as perdas por volatilização de amônia decorrentes do uso de uréia variaram de 1% (com dias chuvosos após a adubação) a 25% do N aplicado. O uso de NBPT proporcionou reduções de 15 a 78% nas perdas por volatilização, dependendo das condições climáticas nos dias posteriores à aplicação de N. A adição de NBPT à uréia ajudou a controlar as perdas de amônia, mas o inibidor foi menos efetivo quando chuvas suficientes para incorporar a uréia no solo ocorreram somente 10 a 15 dias, ou mais, após a aplicação dos fertilizantes.

Há controvérsias quanto à eficiência da correção da acidez da camada subsuperficial pela aplicação de calcário na presença de materiais vegetais de plantas de cobertura e faltam informações sobre os mecanismos envolvidos no processo. O objetivo deste estudo foi quantificar a contribuição dos materiais vegetais de plantas de cobertura, com ênfase nos seus conteúdos de ácidos orgânicos de baixa massa molar e nutrientes solúveis, sobre a mobilização, no perfil do solo, dos produtos da dissolução do calcário aplicado em superfície. Foram desenvolvidos dois experimentos em laboratório, usando colunas de PVC de 30 cm de altura com amostras deformadas de um Latossolo Vermelho textura muito argilosa. Os tratamentos constituíram-se da aplicação isolada ou conjunta de calcário para atingir 80 % de saturação por bases (6,1 t ha-1) e de materiais vegetais (20 t ha-1) de nabo forrageiro e aveia-preta, bem como de soluções de ácidos orgânicos e sais neutros aproximadamente equivalentes ao conteúdo dessas espécies. A calagem isolada ou em associação com os materiais vegetais promoveu a redução da acidez somente na camada superficial (0-8 cm). A baixa taxa de recuperação dos ácidos orgânicos adicionados (< 7,2 %) indica que essas substâncias presentes na massa seca do nabo forrageiro foram rapidamente degradadas por microrganismos ou adsorvidas aos colóides do solo, tendo pouca influência na mobilização de cátions. Parte substancial do efeito sobre a mobilização de íons pela aplicação de material vegetal de nabo forrageiro se deveu à composição química do material, em vista da alta solubilidade em água dos cátions (65 a 71 %) e ânions (84 %). Além disso, a solução de sais foi o tratamento mais eficiente para deslocar o Al do solo para as soluções percoladas. A adição de materiais vegetais teve pouca influência na mobilização no perfil do solo dos produtos da dissolução do calcário aplicado em superfície.The efficiency of subsurface acidity alleviation by surface liming in the presence of crop residues is controversial and more information is needed. The objective of this study was to quantify the contribution of cover crop residues, regarding their contents of low molar mass organic acids and soluble nutrients to mobilization in the soil profile of the reaction products of surface-applied lime. Two experiments were carried out under laboratory conditions using 30 cm high PVC cylinders filled with acid and clayey Red Latosol. Treatments consisted of liming to 80 % soil base saturation (6.1 t ha-1) solely as or liming combined with radish (Raphanus sativus) or with black oat (Avena strigosa) crop residues (20 t ha-1), as well as the equivalent amounts of organic acids and inorganic salts present in the plant materials. Lime application alone or associated with plant material reduced soil acidity in the 0-8 cm surface layer only. The low recovery of organic acids added to the soils (< 7.2 %) indicates that the acids were rapidly metabolized or adsorbed to the soil colloidal fraction, which explains the small effect on cation mobilization. A substantial part of the ion mobilization in the soil and leached solution after application of crop residues was probably due to the plant-own inorganic ion content due to the high water solubility: 65 to 71 % for cations and 84 % for anions. The largest amount of aluminum displaced from the soil was caused by the application of inorganic salt solutions. The presence of plant residues had little effect on the mobilization of the reaction products of surface-applied lime in the soil profile.

A produção e a qualidade da forragem do capim-coastcross cultivado em Latossolo Vermelho Distroférrico foram avaliadas no período de novembro a abril dos anos de 1998-1999 e 1999-2000, em São Carlos, SP, sob clima tropical de altitude. O delineamento experimental utilizado foi o de blocos casualizados, com quatro repetições, em parcelas subdivididas. Nas parcelas foram distribuídos dez tratamentos, organizados em esquema fatorial 2 x 5 (duas fontes de nitrogênio: uréia e nitrato de amônia; e cinco doses: 0, 25, 50, 100, 200 kg N/ha/corte, em cinco cortes consecutivos - medidas repetidas). Na análise, utilizaram-se o procedimento MIXED do SAS e o modelo misto usual para análises de medidas repetidas. A adubação nitrogenada aumentou a produção de MS, o teor de PB, a digestibilidade in vitro da MS e o teor de nitrato e reduziu os teores de MS e FDN na forragem do capim-coastcross. No primeiro ano, as doses de N associadas a 80% da produção máxima de forragem foram de 88 e 78 kg N ha/corte, correspondendo a produções de MS de 2.769 e 3.202 kg N/ha/corte, respectivamente, para uréia e nitrato de amônio. No segundo ano, as doses foram de 91 e 116 kg N/ha/corte e resultaram em produções de MS de 2.312 e 3.072.8 kg N/ha/corte, na mesma ordem.Forage production and quality of coastcross grown on a dark red latosol (Hapludox) were evaluated in 1998-1999 and 1999-2000, from November to April, in São Carlos, São Paulo state, Brazil, under a tropical altitude climate. The experimental design was a randomized block design, in a split-plot arrangement, with four replications. In the main plots were allocated ten treatments, organized in a 2 ´ 5-factorial scheme (two nitrogen sources: urea and ammonium nitrate, and five N levels: 0, 25, 50, 100, and 200 kg N/ha/cutting, in five consecutive cuttings - repeated measures). Data were analyzed by MIXED procedure of SAS, using a repeated measure model. Nitrogen fertilization increased DM yield, CP concentration, in vitro DM digestibility, and nitrate concentration, and reduced DM and NDF concentrations in the forage. In the first year, N doses associated with 80% of the maximum forage yield were 88 and 78 kg/ha, corresponding to average dry matter yields of 2,769 and 3,202 kg N/ha/cutting, respectively, for urea and ammonium nitrate. In the second year, N doses which produced 80% of the maximum forage yield were 91and 116 kg/ha, respectively, for urea and ammonium nitrate, corresponding to average DM yields of 2,312 and 3,073 kg ha/cutting, respectively.

O objetivo deste trabalho foi avaliar, em laboratório, a mineralização líquida do N orgânico de dois lodos de curtume em solos. Um Latossolo Vermelho acriférrico e um Latossolo Vermelho-Amarelo distrófico receberam quatro doses de cada lodo (0, 595, 1.190 e 1.785 kg ha-1 de N): do efluente de caleiro (LCL), com concentração baixíssima de crômio e do decantador primário (LCR), com 17 g kg-1 de Cr. As misturas solos+lodos foram lixiviadas após incubação por períodos crescentes até 132 dias em colunas de percolação. Um modelo cinético de primeira ordem descreveu a mineralização líquida do N (R² = 0,967** a 0,998**). Aplicações de LCR, em comparação às de LCL, diminuíram a porcentagem acumulada do N mineralizado proveniente do lodo (médias de 4 e 35,5%), reduziram o valor da taxa constante de mineralização (médias de 0,0048 e 0,028 dia-1) e aumentaram substancialmente o tempo de meia-vida (T1/2) (variação de 100 a 267 e de 19 a 29 dias), o que indica inibição da mineralização do N, possivelmente em decorrência das elevadas concentrações de Cr daquele lodo. Uma análise detalhada das curvas de mineralização com a aplicação de LCR apontou a existência de três fases, que provavelmente representam adaptações sucessivas da microbiota a diferentes frações recalcitrantes do lodo.The objective of this work was to evaluate nitrogen mineralization in two tannery sludges in soils. A Rhodic Acrustox and a Typic Haplustox received four levels of each sludge (0, 595, 1,190 and 1,785 kg ha-1 N): a liming sludge (LCL), comprising very low chromium contents and a primary sludge (LCR), with 17 g kg-1 Cr. Soil-sludge mixture was placed in percolation tubes and periodically leached after incubation up to 132 days. First order kinetic model described the nitrogen net mineralization (R² = 0.967** to 0.998**). Compared to LCL, the addition of LCR caused a decrease in the percentages of the accumulated mineralized nitrogen from sludges (averages 35.5 and 4.0%, respectively), a decrease in the mineralization rates constants (averages 0.028 and 0.0048 day-1) and substantially increased the T1/2 (ranging from 267 to 100 and from 29 to 19 days), indicating N mineralization inhibition, possible due to the high chromium content of that sludge. A detailed analysis of mineralization curves with LCR applications pointed that there are three phases that probably represent successive adaptations of the microbiota to different recalcitrant fractions.

The aviation industry has set ambitious goals to reduce carbon emissions in coming decades. The strategy involves the use of sustainable biofuels, aiming to achieve benefits from environmental, social, and economic perspectives. In this context, Brazilian conditions are favorable, with a mature agroindustry that regularly produces automotive biofuel largely adopted by Brazilian road vehicles, while air transportation has been growing at an accelerating pace and a modern aircraft industry is in place. This paper presents the main conclusions and recommendations from a broad assessment of the technological, economic, and sustainability challenges and opportunities associated with the development of drop-in aviation biofuels in Brazil. It was written by a research team that prepared the initial reports and conducted eight workshops with the active participation of more than 30 stakeholders encompassing the private sector, government institutions, NGOs, and academia. The main outcome was a set of guidelines for establishing a new biofuels industry, including recommendations for (a) filling the identified research and development knowledge gaps in the production of sustainable feedstock; (b) overcoming the barriers in conversion technology, including scaling-up issues; (c) promoting greater involvement and interaction between private and government stakeholders; and (d) creating a national strategy to promote the development of aviation biofuels.

Objetivou-se verificar possíveis perdas de N-nitrato em profundidade, em experimentos conduzidos no período das chuvas em dois anos consecutivos em pastagem de capim-coastcross (Cynodon dactylon cv. coastcross) instalada sobre Latossolo Vermelho Distrófico típico, na região de São Carlos, SP. Foram determinados os teores de nitrato no perfil do solo, após aplicações superficiais de 125, 250, 500 e 1.000 kg ha-1ano-1 de nitrogênio, na forma de uréia e nitrato de amônio, parcelados em cinco vezes, até a profundidade de 160 cm. Em 1999, a determinação dos teores de N-nitrato após o quinto corte do capim, indicou maiores teores para nitrato de amônio, em especial na maior dose de N. As maiores variações ocorreram até 100 cm. Em 2000, foram medidos semanalmente os teores de N-nitrato no perfil do solo nos tratamentos com as doses de 500 e 1.000 kg ha-1ano-1 de N. As maiores variações (de 0 a 81,2 mg kg-1 de N-NO3-) ocorreram até 40 cm de profundidade. Os resultados indicam não haver risco de contaminação do lençol freático em solos profundos de textura média ocupados por gramínea tropical manejada intensivamente quando não se utilizam adubações nitrogenadas superiores à capacidade de ciclagem da forrageira e quando se considera o potencial de fornecimento do solo.The goal of this work was to assess nitrate losses in the soil profile. Nitrate concentration was determined in the profile of a soil under coastcross pasture (Cynodon dactylon cv. Coastcross), in São Carlos, SP, Brazil, in the rainy season of two consecutive years. The soil was a dark red Latosol (Hapludox), affected by tropical altitude climate. Nitrogen doses of 125, 250, 500 and 1,000 kg ha-1 year-1, as urea or ammonium nitrate were broadcasted splited in five applications. Soil nitrate concentration was determined down to 160 cm depth. In 1999, nitrate level varied according to N sources, reaching higher figure in soil that had received 1,000 kg ha-1 year-1 of N. The highest NO3-N concentration occurred in soil layer down to 100 cm depth, in the plots treated with ammonium nitrate. In 2000, nitrate-N was measured weekly in the soil profile, in treatments with 500 and 1,000 kg ha-1 year-1. The greatest variations, from 0 to 81.2 mg kg-1 of NO3-N, occurred down to 40 cm depth. Results point to low danger of water table contamination in deep medium textured soils of intensively well managed tropical grass pasture, using N doses lower than the forage N cycling capacity and considering the potential soil N sources.

Em pastagens manejadas intensivamente, que recebem altas doses de nutrientes, é necessário conhecer sua extração para auxiliar as recomendações de adubação. Este trabalho teve a finalidade de avaliar os nutrientes extraídos pela fitomassa de capim-marandu (Brachiaria brizantha (Hochst ex. A. Rich.) Stapf cv. Marandu) submetido a fontes e doses de N e seu efeito na recuperação aparente do N, em experimento conduzido em Latossolo Vermelho Distrófico típico, sob condições tropicais. Foram aplicadas três doses de N: 50, 100 e 200 kg ha-1 corte-1, nas formas de uréia e de nitrato de amônio (NA), após quatro cortes consecutivos na estação chuvosa, além da testemunha. Houve aumento na extração dos nutrientes com doses crescentes de N, com valores elevados para K e N. As extrações dos macronutrientes foram maiores para K seguidas de N, Ca, Mg, P e S e dos micronutrientes na seguinte ordem decrescente: Fe, Mn, Zn e Cu. A recuperação média do N, pela forragem, de todas as doses da uréia foi de 84% da obtida com o NA, para o qual variou entre 38 e 51%. Em capim-marandu com produções elevadas de forragem, a extração de K é grande e a recuperação média de N da uréia é inferior à média do nitrato de amônio.Data about nutrient extraction in pastures receiving high rates of nutrients are important to help to define fertilizer recommendations. The present work had the objective of evaluating nutrient extraction and N recovery by marandu grass (Brachiaria brizantha (Hochst ex. A. Rich.) Stapf cv. Marandu) treated with different sources and rates of N, grown on a dark red latosol (Hapludox), under tropical conditions, in Brazil. Three rates of N were surface-applied: 50, 100 and 200 kg ha-1cutting-1 as urea or ammonium nitrate, after four consecutive cuttings, during the rainy season. Nutrient extraction increased with increasing nitrogen rates, with high values mainly for K and N. When forage yield was high (treatment with 400 kg ha-1 year-1 of N) and for both fertilizer, macronutrient extraction was greater for K and N, followed by Ca, Mg, P, and S. Micronutrient extraction decreased in the following order: Fe, Mn, Zn, and Cu. Mean N recovery, by forage, from urea was of about 84% of that of ammonium nitrate, which varied from 38 to 51% of applied N. Large amounts of K are extract for marandu grass with high forage yield, and mean urea-N recovery is lower than for ammonium nitrate-N.

Given their potential for biological nitrogen fixation, legumes used as green manure are an alternative source of nitrogen to crops, and can supplement or even replace mineral nitrogen fertilization. The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulphate (AS) was evaluated using the 15N tracer technique. Amounts of 195.8 kg and 70 kg N per hectare, respectively, of sunn hemp and AS were added in the following treatments: without green manure and without AS; without green manure, with AS -15N; with green manure-15N and with AS; with green manure-15N, without AS; with green manure and with AS-15N. Four samples from the leaves +3 were collected and 2 m of the sugar cane row were harvested to estimate crop yield. The results for N contents (g kg-1), isotopic abundance of N (atoms % 15N) in leaf +3 samples, and sugarcane productivity were used to calculate cumulative N, nitrogen in the plant derived from the fertilizer-Ndff (% and kg ha-1), as well as percent recovery of fertilizer N (R%). Sugarcane was analysed and pol and total recovered sugar calculated. The highest Ndff percentages were observed eight months after sugarcane planting for treatments containing green manure without mineral N, and green manure with mineral N, at 15.3 and 18.4%, respectively. The best nitrogen recovery was observed during harvest, 18 months after planting; the treatment containing mineral fertilizer showed 34.4% recovery, while the sum between mineral N plus green manure N showed 40.0%. Treatments containing green manure plus mineral N changed soil attributes, by increasing Ca and Mg contents, sum of bases, pH, and base saturation, and decreasing potential acidity. In the plant, those treatments increased Ca and K contents.

Sulfato de amônio (SA) e uréia (U) marcados com 15N foram aplicados na cultura do milho, em sucessão à aveia preta (Avena strigosa Schieb.), no sistema plantio direto, 43 dias antes e 31 dias depois da semeadura, na dose de 80 kg ha-1 de N, incorporados a 5-7 cm de profundidade, em sulcos espaçados de 0,8 m, nas entrelinhas do milho. O objetivo foi quantificar o N dos fertilizantes imobilizado no solo (15N-orgânico), no sulco de adubação, e o N-recuperado na planta nos estádios de 5-6 folhas, 11-12 folhas, florescimento e maturação fisiológica. O delineamento experimental foi inteiramente casualizado com parcelas subdivididas e três repetições. As parcelas foram constituídas das fontes U e SA, e as subparcelas, das épocas de aplicação de N. O experimento foi realizado em Latossolo Vermelho ácrico típico fase cerrado subcadocifólio, na Fazenda Floresta do Lobo-Pinusplan, em Uberlândia (MG). Na aplicação em pré-semeadura, a máxima imobilização foi observada aos 19 dias da aplicação do SA (13,3 kg ha-1 ou 16,6 % do N-aplicado) e aos 40 dias da aplicação da U (13,7 kg ha-1 ou 17,1 % do N-aplicado). A maior quantidade de N fertilizante assimilado pela planta ocorreu entre os estádios de 5-6 e 11-12 folhas (44,1 e 23,4 % do N-SA e N-U, respectivamente). Na aplicação em cobertura, a imobilização do N-SA foi inferior a 3,5 % do N-aplicado, enquanto a imobilização do N-U foi de 9,9 kg ha-1 e 7,9 kg ha-1, respectivamente, nos estádios de 11-12 folhas e florescimento. Até o estádio de maturação fisiológica da cultura, 61,8 % do N-SA e 42,0 % do N-U foram recuperados pelo milho. Em média, nos estádios de 11-12 folhas e de florescimento, para cada kg de N-SA imobilizado, as plantas de milho recuperaram 8,0 e 16,7 kg ha-1 de N fertilizante em pré-semeadura e cobertura, respectivamente. Nos tratamentos com U, a média foi de 3,1 kg ha-1, independentemente da época de aplicação. As produtividades de grãos obtidas com SA e U, independentemente da época de aplicação, foram de 7.824 kg ha-1 e 6.977 kg ha-1, respectivamente. Na adubação em pré-semeadura do milho, o SA apresentou maior rapidez na ciclagem do N imobilizado-mineralizado ("turnover"), em relação a U, e, conseqüentemente, causou maior assimilação do N pela cultura. Em cobertura, no sulco de adubação, somente houve imobilização do N-U, retardando a sua assimilação pela planta.In order to evaluate the amount of immobilized N and the amount of N-fertilizer recovered by corn plants at the stages:5-6 leaves, 11-12 leaves, flowering, and physiologic maturation, ammonium sulfate (AS) and urea (U), labeled with 15N, were applied to maize in a no-till system 43 days before planting and 31 days after planting at a single rate of 80 kg ha-1 of N incorporated at 5-7 cm depth and in a spacing of 0.8 m. Corn was sown after black oat (Avena strigosa Schieb.). The treatments were applied in split-plots set up in the completely randomized design with three replications. The original plots with two N sources were halved for the application time factor (pre-planting and top-dressing application). The field experiment was carried out on a Typic Acrustox on the farm Floresta do Lobo, Uberlandia, state of Minas Gerais. Results show that in pre-planting application treatments the maximum N-AS immobilization occurred 19 days after the fertilizer application (13.3 kg ha-1 or 16.6 % of N applied) whereas the maximum immobilization of N-U occurred 40 days after fertilizer application (13.7 kg ha-1 or 17.1 % of N applied). The highest amount of 15N-fertilizer taken up by corn plants was observed between the stages 5-6 leaves and 11-12 leaves, at ratios of 44.1 % of N-AS and 23.4 % of N-U. The immobilized N in the treatment with top-dressed AS was lower than 3.5 % of the applied N whereas in the treatment with top-dressed U it was 9.9 kg ha-1 and 7.9 kg ha-1 at the stages 11-12 leaves and flowering, respectively. The recovery of N-fertilizer measured at the stage of physiologic maturation in the treatments with AS and U was 61.8 % and 42.0 % respectively. The 15N-fertilizer amounts recovered by corn plants per kg of 15N immobilized in the treatments with AS were 8.0 kg ha-1 and 16.7 kg ha-1 respectively for pre-planting and top-dressing application. Independent of the application time, the ratio N-fertilizer recovery/immobilized N-fertilizer in the U treatments was 3.1 kg ha-1. The highest corn yields were obtained in the AS treatments (grain mean of 7,824 kg ha-1), independent of the application time. The average crop yield in the treatments with U in both application periods was 6,977 kg ha-1. These results show that when the fertilizers were applied at pre-planting the immobilization-mineralization turnover was faster in the AS than in the U treatment. Consequently, the N-assimilation by corn plants was higher in the AS treatments. For top-dressing application, only U was significantly immobilized.

Os processos fisiológicos das plantas são afetados pelo balanço de cátions e ânions absorvidos. O objetivo deste trabalho foi determinar a absorção de cátions e ânions quando plantas de capim-coastcross receberam doses elevadas de nitrogênio. O delineamento experimental foi o de blocos casualizados com quatro repetições, num esquema fatorial 2x5 - duas fontes de N: uréia e nitrato de amônio, e cinco doses de N: 0, 25, 50, 100, 200 kg ha-1 corte-1 . As doses de N foram aplicadas após cada corte num total de cinco cortes, durante a época das chuvas. A absorção de cátions e de ânions pelo capim-coastcross aumentou com o acréscimo das doses de N dos dois fertilizantes, sendo maior com o nitrato de amônio. Com doses crescentes de N, verificou-se entre os cátions maior absorção do K+, e do Cl- entre os ânions. Com exceção do N, a absorção do K+ foi superior à dos demais nutrientes, com redução no teor relativo de cálcio. Doses altas de N aplicadas em capim-coastcross, na forma de uréia ou de nitrato de amônio, favorecem a absorção de cátions e de ânions.Physiologic processes of plants are affected by uptake of cations and anions. The aim of this work was to determine the uptake of cations and anions when plants of coastcross grass received high doses of nitrogen. The experimental design was a randomized block, in a 2x5 factorial arrangement - two N sources: urea and ammonium nitrate and five N rates: 0, 25, 50, 100, and 200 kg ha-1 cutting-1 - with four replications. Treatments were applied after each of five consecutive cutting in the rainy season. Uptake of cations and anions by coastcross grass increased with increasing of N rates with both fertilizers, but was higher with ammonium nitrate. Increasing rates of N caused higher K+ uptake in relation to other cations, and in Cl- among the anions. Except for N, K+ uptake was greater than that of other nutrients, with a reduction on the relative content of Ca2+. High doses of N as urea or ammonium nitrate applied on coastcross grass favor absorption of cations and anions.

O valor de comercialização no mercado de fruta fresca e a maior resistência a doenças, como o declínio e a clorose variegada dos citros, comparado às variedades de laranjas, estimularam a produção de tangerinas e de 'Murcott' na última década. Por outro lado, faltam informações seguras para o diagnóstico das necessidades de adubação para esses cítricos. Assim, o presente trabalho foi planejado com os objetivos de estudar a demanda por nutrientes e estabelecer doses de fertilizantes para maximizar a produtividade e a qualidade dos frutos de tangor 'Murcott' e definir critérios de diagnóstico da análise de folhas para o manejo nutricional dessas plantas. O experimento foi desenvolvido durante seis anos, num pomar comercial da variedade Murcott sobre limoeiro 'Cravo', com 4 anos de idade. Os tratamentos foram arranjados no delineamento fatorial fracionado, do tipo ½ (4³) e constituídos por níveis de N (30; 100; 170 e 240 kg N ha-1), de P (20; 80; 140 e 200 kg ha-1 de P2O5) e de K (30; 110; 190 e 270 kg ha-1 de K2O). O N aumentou a produção média do período, enquanto o efeito do K foi negativo. Não houve efeito significativo para doses de P. A produção máxima de frutos foi obtida com as doses 155; 20 e 30 kg ha-1, respectivamente, de N, P2O5 e K2O, enquanto, para otimizar o tamanho dos frutos, foi necessário elevar a doses de K para 270 kg ha-1 de K2O. Outras características de qualidade dos frutos também foram estudas, bem como critérios para a interpretação de resultados de análises de folhas.The Brazilian production of mandarins and 'Murcott' tangor has increased during the 1990's due to superior value of fruit for commercialization and higher resistance to citrus diseases, i.e.: citrus variegated chlorosis, compared to sweet oranges. On the other hand, there is a lack of information to establish optimum nutrient requirements for such varieties. Therefore, the present work aimed to study the response of 'Murcott' trees to NPK and their interactions to establish fertilizer rates for maximum fruit yield and superior quality, and to define diagnostic criteria based on leaf analysis for best nutrient management. The experiment was conducted for six-years in a commercial orchard of 4-yr-old 'Murcott' tangor on Rangpur lime rootstock, which was arranged in a fractional factorial design of the ½ (4³) type. Treatments consisted of four rates of N (30, 100, 170, and 240 kg N ha-1), P (20, 80, 140, and 200 kg ha-1 de P2O5), and K (30, 110, 190, and 270 kg ha-1 de K2O). Fruits, measuring about 3-cm diameter, were thinned yearly to maintain a minimum distance of 20-cm between them in the canopy. Average fruit yield increased with increased N rates and decreased with K rates. There was no significant response of P fertilization on fruit yield. Maximum fruit yield was attained at fertilizer rates of N = 155, P2O5 = 20, and K2O = 30 kg ha-1, whereas optimum fruit size was attained at K2O =& 270 kg ha-1. Tree growth and other fruit quality characteristics were evaluated, as well as the criteria for interpretation of leaf analysis.

Com a finalidade de determinar as doses de N, P e K para obter a máxima produção econômica de forragem para a linhagem UPF 87111, nos sistemas de plantio convencional e plantio com cobertura morta, foram instalados experimentos em Latossolo Vermelho-Amarelo Distrófico típico, em São Carlos, SP. O delineamento experimental foi o fatorial fracionado tipo (1/2)4³ com dois blocos ao acaso, total de 32 parcelas, sem repetição. Os tratamentos foram quatro doses de N e de K2O (0, 70, 140 e 210 kg ha-1), como uréia e cloreto de potássio, e quatro doses de P2O5 (0, 60, 120, 180 kg ha-1), como superfosfato triplo. As doses de N, P e K para a produção de forragem de aveia com máxima receita líquida foram, em kg ha-1 para o plantio convencional: N =160, P2O5 = 180, K2O = 180 e para o plantio com cobertura morta: N = 200, P2O5 = 120, K2O = 95, com produções de matéria seca de, respectivamente, 7.398 kg ha-1 e 8.111 kg ha-1.Two field experiments were carried out on a Red Yellow Latosol (Hapludox), at São Carlos, SP in order to determine the rates of N, P, and K to obtain maximum economic return per area of forage yield for the oat line UPF 87111 grown in two planting systems: conventional tillage and mulch-covered. The experimental design was a fractionated factorial type (1/2)4³, with two blocks. The treatments consisted of four rates of N and K2O (0, 70, 140, and 210 kg ha-1), using urea and potassium chloride, and four rates of P2O5 (0, 60, 120 and 180 kg ha-1), using triple superphosphate. The N, P, and K rates for maximum profit were, in kg ha-1 for conventional tillage: N = 160, P2O5 = 180, K2O = 180; and for mulch-covered: N = 200, P2O5 = 120, K2O = 95 resulting in forage yields of, respectively, 7,398 and 8,111 kg ha-1 of dry matter. In both planting systems, the use of nitrogen resulted in higher forage yield per unit of applied fertilizer.

Foi conduzido experimento em Latossolo Vermelho Distrófico típico, em São Carlos, SP, para avaliar o efeito de doses e fontes de nitrogênio (N), no teor, extração dos nutrientes e na recuperação do N aplicado em capim-coastcross (Cynodon dactylon cv. Coastcross). Foram aplicadas sobre a superfície do solo cinco doses de N (0, 25, 50, 100 e 200 kg ha-1corte-1), na forma de uréia ou de nitrato de amônio, em cinco períodos consecutivos, durante a estação chuvosa. Foram determinados os teores e calculada a extração de nutrientes e a recuperação do N. Houve aumento na extração dos nutrientes com o aumento da produção de forragem, com elevados valores principalmente para N e K. Para altas produções de forragem (tratamento 500 kg ha-1 ano-1de N) e para as duas fontes de nitrogênio as extrações dos macronutrientes foram maiores para K e N, seguidas de Ca, S, P e Mg e dos micronutrientes Fe, Mn, Zn e Cu. A recuperação média de N da uréia foi de 68% do N obtido do nitrato de amônio, que variou, em média, de 68 a 75%.Contents, nutrient extraction, and apparent applied N recovery were determined in a coastcross pasture established on a dark red latosol (Hapludox), in São Carlos, SP, Brazil, receiving five rates of N as urea or amonium nitrate, surface-applied (0, 25, 50, 100, and 200 kg ha-1 cutting-1) in five consecutive periods, during the rainy season. Nutrient extraction increased with increasing nitrogen rates. When forage yield was high (treatment with 500 kg ha-1 year-1 of N) and for both fertilizers, macronutrient extraction was greater for K and N, followed by Ca, S, P, and Mg. Micronutrient extraction occurred in the following decreasing order: Fe, Mn, Zn, and Cu. Nitrogen recoveries from urea and ammonium nitrate surface-applied on coastcross pasture were calculated. Significant differences occurred within periods (P