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Colorful flower patterns are key signals to attract pollinators. To produce such motifs, plants specify boundaries dividing petals into subdomains where cells develop distinctive pigmentations, shapes, and textures. While some transcription factors and biosynthetic pathways behind these characteristics are well studied, the upstream processes restricting their activities to specific petal regions remain enigmatic. Here, we unveil that the petal surface of Hibiscus trionum, an emerging model featuring a bullseye on its corolla, is prepatterned as the bullseye boundary position is specified long before it becomes visible. Using a computational model, we explore how pattern proportions are maintained while petals experience a 100-fold size increase. Exploiting transgenic lines and natural variants, we show that plants can regulate boundary position during the prepatterning phase or modulate growth on either side of this boundary later in development to vary bullseye proportions. Such modifications are functionally relevant, as buff-tailed bumblebees can reliably identify food sources based on bullseye size and prefer certain pattern proportions. [ABSTRACT FROM AUTHOR]

Summary: Hibiscus glaber is a symbolically significant endemic species found exclusively on the Ogasawara (Bonin) Islands. It is one of the main tree species in the natural forests of the island. This species is illustrated here, and its natural history, ecology, pollination, and cultivation are described. [ABSTRACT FROM AUTHOR]

True mangroves and mangrove associates are salt‐tolerant trees and shrubs that serve a critical role in preserving the ecological balance of tropical and subtropical estuarine environments. However, they are not immune to the damage caused by fungal pathogens. In this study, leaves exhibiting leaf spot symptoms were gathered from mangrove estuary and forests in Prachuap Khiri Khan, Thailand. Through morphological and multilocus phylogenetic analyses (LSU, ITS, RPB2 and TUB2) along with genealogical concordance phylogenetic species recognition, we identified Allophoma acanthi sp. nov. from Acanthus ilicifolius. Additionally, A. minor was identified as the causative agent of leaf spots in true mangrove and mangrove associate species, namely, Acanthus ilicifolius, Combretum sp., Hibiscus tiliaceus, Intsia bijuga, Lumnitzera racemosa and Sonneratia alba. We also provide evidence to support the synonymization of A. thunbergiae with A. minor. All eight isolates of Allophoma species in this study demonstrated pathogenicity to their original hosts with varied virulence, confirming Koch's postulates. Our findings contribute to the understanding of fungal pathogens affecting true mangroves and mangrove associates. [ABSTRACT FROM AUTHOR]

Hibisceae subtribe Trionum is the largest clade within Hibiscus s.l. and contains genera with a wide range of growth forms: herbaceous (Abelmoschus), shrubs (Malvaviscus), trees (Wercklea), and exceptionally lianas. The rare and localized Thepparatia species are unique in that they combine a lianescent habit and trioecy. Several problems have delayed the study of Hibisceae classification including low genetic variability, lack of synapomorphic morphological characters, transoceanic dispersal events, and allopolyploidy. Here, we present a comprehensive phylogenomic hypothesis of Thepparatia based on analyses of the maternally inherited chloroplast and biparentally inherited nuclear ribosomal cistron sequences, and compare these results with its speciation, karyology, and breeding system. Chloroplast genomes in subtribe Trionum were highly conserved except in Abelmoschus where four additional genes were duplicated. Recent diploid interspecific hybridization is suggested in Thepparatia sipsongpannaensis between T. fragrans and T. scandens during the Pleistocene, around 640 000 years ago. Our observations also indicate Thepparatia have a trioecious breeding system that combined with a unique habit, and a highly localized distribution pattern, have created cross-pollination barriers and ecological specialization. This suggests hybridization events are rare but evolutionary significant in subtribe Trionum. Finally, our study supports important nomenclature changes: the synonymization of Hibiscus austroyunnanensis with Thepparatia fragrans and the publication of a new species, Thepparatia sipsongpannaensis. [ABSTRACT FROM AUTHOR]

Wound is defined as the damage to biological tissues including skin, mucous membranes and organ tissues. The acute wound heals in less than 4 weeks without complications, while a chronic wound takes longer than 6 weeks to heal. Wound healing occurs in 4 phases, namely, coagulation, inflammatory, proliferative and remodeling phases. Triclosan and benzalkonium chloride are commonly used as skin disinfectants in wound healing. However, they cause allergic contact dermatitis and antibiotic resistance. Medicinal plants are widely studied due to the limited availability of wound healing agents. The present review included six commonly available medicinal plants in Malaysia such as Aloe barbadensis Miller, Carica papaya Linn., Centella asiatica Linn., Cymbopogon nardus Linn., Ficus benghalensis Linn. and Hibiscus rosa sinensis Linn. Various search engines and databases were used to obtain the scientific findings, including Google Scholar, ScienceDirect, PubMed Central and Research Gate. The review discussed the possible mechanism of action of medicinal plants and their active constituents in the wound healing process. In addition, their application in nanotechnology and wound dressings was also discussed in detail. [ABSTRACT FROM AUTHOR]

The Hibiscus sabdariffa (H. sabdariffa) belongs to the Malvaceae family, and originates from Africa. It is characterized by a high polyphenols content (e.g., quercetin and anthocyanins), and has recognized bioactivity, that makes this plant an interesting resource for possible addition of its extracts to dairy beverages and obtain functional beverages. Nonetheless, it may be observed that many of these phenolic compounds are not stable depending on the environmental conditions. In this work, we propose the use of nanostructured lipid carriers (NLC) as a carrier for the loading of H. sabdariffa extracts, for further incorporation in daily beverages. Polyphenols-enriched extracts from H. sabdariffa were obtained using two distinct extraction approaches: by (i) microwave-assisted extraction (MAE), or by (ii) pressurized liquid extraction (PLE). The obtained extracts were then loaded into NLC to obtain two distinct samples identified as: HS-MAE-NLC and HS-PLE-NLC. The developed nanoparticles were then incorporated into a dairy beverage for the production of fortified milks identified with the same names. The long-term stability, texture properties and in vitro release profile were evaluated. The results show that the fortified milks, HS-MAE-NLC and HS-PLE-NLC, were stable under stress conditions attributed to the enhanced stability provided by the protein content present in the milk. The in vitro release profile of quercetin and anthocyanins from NLC-enriched dairy beverage was more prolonged than the milk containing the non-loaded extract, fitting better to the Korsmeyers–Peppas model. From our results, NLC may be considered a potential approach to formulate photoprotective, anti-inflammatory, and antioxidant bioactives from H. sabdariffa adding the extracts into dairy beverages to increase their bioavailability, and bioactivity. [ABSTRACT FROM AUTHOR]

Hinduism has profound religious beliefs and cultural connections with many plants employed in various religious rituals to worship gods and goddesses. Most of these plants are believed to have curative properties for many ailments. In view of the Hindu population decline in Bangladesh during the last decades, there is a need to gather ethnopharmacological data on these sacred plants. This study focuses on documenting the therapeutic uses of plants significant to Hindu practices in southwestern Bangladesh. Through free listing interviews with 28 Hindu participants, medico-religious data on plants were collected using open-ended and semi-structured questionnaires. Results revealed 56 plant species from 32 botanical families as religiously sacred, with Aegle marmelos being most culturally significant. The study identified 89 diseases treated by these plants, with Curcuma longa being the most cited species, followed by Hibiscus rosa-sinensis. It introduced two new ethnomedicinal plant species, Oxystelma esculentum and Reinwardtia indica, previously unreported in Bangladesh. Additionally, new medicinal uses were uncovered, highlighting the intricate relationship between culture, religion, and traditional medicinal knowledge within the Bangladeshi Hindu community. This study represents the first attempt to compile a comprehensive list of plants utilized in Hindu religious practices in Bangladesh. [ABSTRACT FROM AUTHOR]

Plants and microbes are closely associated with each other in their ecological niches. Much has been studied about plant–microbe interactions, but little is known about the effect of phytochemicals on microbes at the molecular level. To access the products of cryptic biosynthetic gene clusters in bacteria, we incorporated an organic extract of hibiscus flowers into the culture media of different Actinobacteria isolated from plant rhizospheres. This approach led to the production of broad-spectrum dithiolopyrrolone (DTP) antibiotics, thiolutin (1) and aureothricin (2), by Streptomyces sp. MBN2-2. The compounds from the hibiscus extract responsible for triggering the production of these two DTPs were found to be hibiscus acid dimethyl ester (3) and hydroxycitric acid 1,3-dimethyl ester (4). It was subsequently found that the addition of either Fe2+ or Fe3+ to culture media induced the production of 1 and 2. The Chrome Azurol S (CAS) assay revealed that 3 and 4 can chelate iron, and therefore, the mechanism leading to the production of thiolutin and aureothricin appears to be related to changes in iron concentration levels. This work supports the idea that phytochemicals can be used to activate the production of cryptic microbial biosynthetic gene clusters and further understand plant–microbe interactions. [ABSTRACT FROM AUTHOR]

Copyright of Online Turkish Journal of Health Sciences (OTJHS) / Online Türk Sağlık Bilimleri Dergisi is the property of Oguz KARABAY and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Isotonic drinks are sports beverages that replenish minerals and rapidly absorbable carbohydrates. We proposed to surpass traditional formulations of isotonic drinks by integrating thermal water, which remains unexploited as a source of electrolytes in isotonic despite the high mineral content. The novel formula combined thermal water with apple juice and hibiscus extract (as sources of sugars and color, respectively, and bioactive compounds). Through comprehensive analysis of chemical, physicochemical, and microbiological properties over 45 days at 4°C and 25°C, the results unveiled an isotonic drink with satisfactory characteristics for this product category: pH of 3.72, 7.93 mg/100 mL of total sugars, and 148.30 mg/100 mL minerals, predominantly sodium (34.99 mg/100 mL) and potassium (73.20 mg/100 mL). It showed significant phenolic compounds, notably chlorogenic acid (2.75±0.10 mg/100 mL), and antioxidant activity (472.72 µmol Trolox eq/100 mL). It maintained a vibrant red hue derived from hibiscus anthocyanins (delphinidin 3-O-sambubioside and cyanidin 3-O-sambubioside, totaling 4.06±0.03 mg/100 mL), and demonstrated microbiological stability throughout the storage period in both temperatures studied. Therefore, by harnessing the potential of thermal water, this study paves the way for a novel approach to developing sports beverages and can serve as a model for countries rich in thermal springs.

ABSTRACTThis study aimed to produce herbal tea using an infusion of Hibiscus with Lemon verbena. Dried Hibiscus calyces and lemon verbena leaves were combined in a percentage of: 90:5 (HL1), 90:10 (HL2), 85:15 (HL3), 80:20 (HL4), 75:25 (HL5), 70:30 (HL6), 65:35 (HL7), 60:40 (HL8), 55:45 (HL9), 50:50 (HL10), respectively, and commercial tea and 100% Hibiscus were used for comparison. The formulated tea was evaluated for pH, total soluble solids (TSS), color density, hue tint, total phenolics, total flavonoids, DPPH radical scavenging capacity and ferric reducing antioxidant power (FRAP), sensory acceptance (7-hedonic scale) and their correlation were determined. No significant differences (p > .05) were found for total soluble solids (TSS). The pH, total flavonoids, total phenolics and antioxidant capacities of blended tea were significantly decreased with the concentrations of calyces. The blended tea had sensory acceptance with incorporation of 10–45% dried lemon verbena leaves, and potential phytochemical and antioxidant capacities.

• Hibiscus , a flowering plant (Malvaceae) renowned for medicinal and aesthetic value. • First in-depth report on the characterization of 19 different Hibiscus germplasms. • Revealed significant morphological, biochemical, and molecular diversity. • A multifaceted analysis of biochemical traits encompassing antioxidant properties. • Exhibited 34.75 % polymorphism among 19 Hibiscus germplasms using four molecular marker systems. Hibiscus , a popular ornamental plant of mallow family Malvaceae, is composed of more than 400 species of flowering plants. It is originally native to tropical Asia (China and India) and grows in warm-temperate, subtropical, and tropical regions worldwide. The basic chromosome number varies from x = 7, 8, 9, 11, 12, 15, 17, 19, 20 and 39. In ancient times, it was only used for religious purposes (as offerings to God), but nowadays, it is considered of great significance as an ornamental as well as a medicinal plant. Important species found in this zone are Hibiscus rosa-sinensis L., H. cannabinus L., H. storckii Seem., H. fragilis DC., H. schizopetalus (Mast.) Hooker. f. along with germplasms like Celia, Kali Jaba, Red Dragon, Cinnamon Girl, Brilliant, etc. have been identified to date. Still, there is a lack of proper documentation and characterization (based on morphological, biochemical, and molecular traits). With this aim in mind, the above research work was considered. On the basis of morphological parameters (plant height, flower weight, type of flower, etc.), Brilliant (T 1), Sudarshan Chakra (T 16), Storckii (T 15), and Golden Doublon (T 8) were similar but diverse from the rest of the germplasms. Likewise, Brilliant (T 1), Golden Doublon (T 8), Celia (T 2), and Versicolor Pinwheel (T 19) were rich in biochemical pigments viz., chlorophyll, carotenoid, lycopene, and anthocyanin content. In contrast, phenol and ascorbic acid were found to be highest in Spider (T 15), and antioxidant properties (DPPH and FRAP assay) were maximum in Oiseau Blue (T 12). Finally, a significant amount of genetic diversity was assessed with molecular markers, and 141 loci were produced using inter simple sequence repeats, start codon targeted, conserved DNA-derived polymorphism, and directed amplification of minisatellite-region DNA marker systems, out of which 49 loci were polymorphic (34.75 % polymorphism). On the basis of molecular diversity analyzed by network plot, unweighted pair group method with arithmetic mean clustering, and principal component analyses, Oiseau Blue (T 12) was found completely diverse from all the germplasms, placed separately in the dendrogram, and thus may be considered as a potential parent for the hybridization programme. [ABSTRACT FROM AUTHOR]

Simple Summary: This study investigated the resistance of Hibiscus mutabilis varieties to the whitefly Bemisia tabaci. It identified the highly resistant variety Jinqiusong (JQS) and moderately resistant Bairihuacai (BRHC), which showed significantly lower B. tabaci populations than the susceptible variety Chongbanbai (CBB). Metabolomic analysis revealed that fifteen key metabolites are linked to resistance, with the phenylpropanoid biosynthesis pathway being critical in defense. Additionally, volatile compounds emitted by the resistant varieties deterred the pest's behavior. The findings offer a theoretical basis for breeding insect-resistant H. mutabilis varieties, providing a sustainable strategy for managing B. tabaci infestations. Hibiscus mutabilis, the city flower of Chengdu, is culturally significant and has nutritional and medicinal benefits. However, frequent infestations of Bemisia tabaci have caused economic losses. This study aimed to identify insect-resistant H. mutabilis varieties. Over two years, varieties like Jinqiusong, Zuiyun, and Zuifurong showed moderate to high resistance based on reproductive indices. Assessments of antixenosis and developmental impacts revealed that adult B. tabaci exhibited low selectivity toward these resistant varieties, indicating a strong repellent effect. Gas chromatography-mass spectrometry analysis identified volatile organic compounds, such as alcohols, alkanes, and terpenes. Notably, 2-ethylhexanol and 6-methylheptanol exhibited repellent properties. Using nontargeted metabolomics, this study compared the metabolite profiles of the insect-resistant variety Jinqiusong (JQS), moderately resistant Bairihuacai (BRHC), and highly susceptible Chongbanbai (CBB) post B. tabaci infestation. Fifteen key metabolites were linked to resistance, emphasizing the phenylpropanoid biosynthesis pathway as crucial in defense. These findings offer a theoretical foundation for breeding insect-resistant H. mutabilis varieties and developing eco-friendly strategies against B. tabaci infestations. [ABSTRACT FROM AUTHOR]

We examined the antibacterial efficacy of streptomycin, hibiscus acid, and their combination against multidrug-resistant Shiga-toxin-producing Escherichia coli (STEC) and Salmonella Typhimurium in mice. We determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for streptomycin, hibiscus acid, and their combination against STEC and Salmonella. Fifteen sets of six mice in each set were utilised: six groups were orally exposed to 4 log10 colony forming units (CFUs) of S. Typhimurium and another six to STEC, and three acted as the controls. Six hours post-inoculation, specific groups of mice received either oral solutions containing hibiscus acid at 5 and 7 mg/ml; streptomycin at 50 and 450 µg/ml; hibiscus acid/streptomycin (5 mg/ml hibiscus acid and 50 µg/ml streptomycin); or isotonic saline. The study determined the MIC and MBC of 7 mg/ml of hibiscus acid; 300 and 450 µg/ml of streptomycin; and two concentrations of hibiscus/streptomycin (3 mg/ml / 20 µg/ml and 5 mg/ml / 50 µg/ml). Interestingly, the mice that were infected and subsequently treated with hibiscus acid at 7 mg/ml alone or in conjunction with streptomycin did not have either STEC or Salmonella in their faecal samples, and none of the mice died. In contrast, the untreated mice and those exclusively treated with streptomycin had the pathogens present in their stool, leading to the mortality of all the subjects. [ABSTRACT FROM AUTHOR]

The article explores Egypt's rich botanical heritage, emphasizing its ancient use of plants in daily life, medicine, and religious rituals. It also discusses the economic importance of medicinal and aromatic plants (MAPs) in modern Egypt, aiming to boost agricultural productivity and foreign income through exports.

We aimed to examine the potential protective effects of Iraqi H. tiliaceus L. chloroform leaves extract on DEN-induced HCC in male Wistar Albino rats. Rats were assigned to four groups, six in each group. Group I: rats were administered a daily oral dose of 1 mL/kg/day of distilled water. Group II: rats were intraperitoneally injected with 70 mg/kg DEN once per week for 10 consecutive weeks. Group III: rats received 250 mg/kg of chloroform leaves extract. Groups IV: the rats were administered 500 mg/kg of chloroform leaves extract, along with their food, for five days per week over 20 weeks, with a subsequent dose of DEN once per week for 10 consecutive weeks. The results indicate that the extract demonstrated a significant reduction (p<0.05) in oxidative stress, pro-inflammatory mediators, and HCC parameters, the extract also had a beneficial effect on liver function tests, and there was a significant elevation (p<0.05) of antioxidant parameters in a dose-dependent manner. This study supports the protective properties of the chloroform extract of Iraqi H. tiliaceus L. leaves in HCC. [ABSTRACT FROM AUTHOR]

Background: The flower colour of H. syriacus 'Qiansiban' transitions from fuchsia to pink–purple and finally to pale purple, thereby enhancing the ornamental value of the cultivars. However, the molecular mechanism underlying this change in flower colour in H. syriacus has not been elucidated. In this study, the transcriptomic data of H. syriacus 'Qiansiban' at five developmental stages were analysed to investigate the impact of flavonoid components on flower colour variation. Additionally, five cDNA libraries were constructed from H. syriacus 'Qiansiban' during critical blooming stages, and the transcriptomes were sequenced to investigate the molecular mechanisms underlying changes in flower colouration. Results: High-performance liquid chromatography‒mass spectrometry detected five anthocyanins in H. syriacus 'Qiansiban', with malvaccin-3-O-glucoside being the predominant compound in the flowers of H. syriacus at different stages, followed by petunigenin-3-O-glucoside. The levels of these five anthocyanins exhibited gradual declines throughout the flowering process. In terms of the composition and profile of flavonoids and flavonols, a total of seven flavonoids were identified: quercetin-3-glucoside, luteolin-7-O-glucoside, Santianol-7-O-glucoside, kaempferol-O-hexosyl-C-hexarbonoside, apigenin-C-diglucoside, luteolin-3,7-diglucoside, and apigenin-7-O-rutinoside. A total of 2,702 DEGs were identified based on the selected reference genome. Based on the enrichment analysis of differentially expressed genes, we identified 9 structural genes (PAL, CHS, FLS, DRF, ANS, CHI, F3H, F3'5'H, and UFGT) and 7 transcription factors (3 MYB, 4 bHLH) associated with flavonoid biosynthesis. The qRT‒PCR results were in good agreement with the high-throughput sequencing data. Conclusion: This study will establish a fundamental basis for elucidating the mechanisms underlying alterations in the flower pigmentation of H. syriacus. [ABSTRACT FROM AUTHOR]

• Green synthesis of agnps from H. tiliaceus L. leaves extract. • Characterization of agnps was done by FE-SEM, HR-TEM, XRD and FT-IR. • Nanoparticles were stable in charge, crystalline in shape, with an average size of 88.10 nm in colloidal form. • AgNPs displayed potential antioxidant activity and broad range of antibacterial activity. • AgNPs have been shown to have potential as a catalyst in the dye degradation process. The present study reports the green synthesis of silver nanoparticles (AgNPs) from aqueous extract of Hibiscus tiliaceus L. leaves and their application in dye degradation, antioxidant, antimicrobial, and anticancer activities. Analysis using Fourier transform infrared (FT-IR) spectroscopy revealed that plant metabolite functional groups had a role in the reduction and stability of AgNPs, and X-ray crystallography (XRD) demonstrated that the AgNPs were in crystal form. The UV–vis spectroscopy, dynamic light scattering (DLS), and zeta potential investigations revealed that the AgNPs were formed with an average size of 88.10 nm in colloidal form and were stable (-49 mV). The field emission scanning electron microscopy (FE-SEM) and high-resolution transmission electron microscopy (HR-TEM) confirmed the shape and size of the AgNPs as spherical with a particle size of 30 - 35 nm, respectively. The AgNPs exhibited potential antioxidant activity by total antioxidant, DPPH, and reducing power assays. The biosynthesized AgNPs displayed a wide range of antibacterial activity on Gram-ve and Gram+ve bacteria by the zone of inhibition assay. AgNPs showed good anticancer activity on MCF-7 cells with an IC 50 value of 65.83 µg/mL. Furthermore, AgNPs acted as potential catalysts in combination with the reducing agent sodium borohydride (NaBH 4) for the degradation of methylene blue (MB), methylene orange (MO), and methylene green (MG) dyes. The degradation efficiency of catalyst AgNPs in the attendance of NaBH 4 for 15 min was noted to be 12.8 %, 26.92 %, and 47.56 % for MO, MB, and MG, respectively. The study concluded that green synthesized AgNPs could be highly applicable as an antioxidant, antimicrobial, and anticancer agents in the biomedical field. Furthermore, AgNPs could be helpful in the remediation of dye effluents. [ABSTRACT FROM AUTHOR]

The mealybug, Phenacoccus solenopsis, is a serious pest of Hibiscus rosa-sinensis. The waxy coating on its body may hinder pesticide penetration, and the extensive use of pesticides is risky to humans and the environment. Considering these drawbacks, fermented plant extracts (FPEs) were explored for their potential use in controlling this pest in a more user and environmentally-friendly manner. FPEs derived from eleven plant materials were evaluated against P. solenopsis for their insecticidal activity, mealybug wax removal potential and phytotoxicity effect on H. rosa-sinensis. Five concentrations of FPE [5, 10, 15, 20, and 25% (w/v)] were prepared. Among the 11 FPEs, FPE derived from ficus, kaffir lime, and turmeric were effective in suppressing P. solenopsis with lethal concentration at 50% (LC50 value) less than 20% concentration. Although ficus FPE was the top performer in the insecticidal assay, it induced medium to very high levels of leaf damage after being treated with 15–25% concentration at 24 and 72 hr post-treatment. A low level of leaf damage was observed in treatment with turmeric and kaffir lime FPEs at 72 hr post-treatment. FPEs could remove wax from the body of P. solenopsis with no significant difference among them. In conclusion, the FPE of ficus, kaffir lime, and turmeric showed promising insecticidal effects against P. solenopsis. [ABSTRACT FROM AUTHOR]

Background: Child malnutrition is a major public health challenge, affecting millions of children worldwide, with alarming proportions of children under five in Benin. The complexity of managing this condition is increased by its potential association with opportunistic pathologies. An interesting approach arises from the use of medicinal plants, to address child malnutrition and its associated pathologies. This study aimed to document the knowledge and practices of Beninese mothers and traditional practitioners with regard to the use of medicinal plants to treat child malnutrition and associated diseases. Methods: A total of 844 Beninese mothers and 201 traditional healers were surveyed between March 2022 and August 2023 in the communes of Karimama, Bopa and Za-Kpota in Benin. The respondents' knowledge of child malnutrition and associated pathologies was explored. The ethnobotanical data collected from the subjects concerned the medicinal recipes used to treat child malnutrition, the medicinal plants that make them up and the methods of use. These data were analyzed using ethnobotanical indices such as the Informant Consensus Factor, the frequency of citation of medicinal recipes types and medicinal plants, and the contribution of plants to medicinal recipes. Results: All respondents cited a total of 82 plant species used to treat child malnutrition and associated diseases. These plants were grouped into 37 botanical families, the most common of which were Fabaceae, Malvaceae and Annonaceae. The leaves were the most commonly used part of the plant species identified. The mothers shared 122 medicinal recipes, ranging from recipes based on a single plant to more complex compositions involving five plants. The most notable plants were Moringa oleifera Lam, Phyllanthus amarus Schumach & Thonn, Senna siamea (Lam.) H.S.Irwin & Barneby, Carica papaya L. and Ocimum gratissimum L. Traditional healers provided 52 plants in 71 recipes, with Moringa oleifera featuring prominently in both single-plant and multiplant formulations. Conclusion: This study made it possible to constitute a rich base of medicinal recipes used against malnutrition and associated pathologies, with the preponderant involvement of certain plant species. It is therefore necessary to deepen research on these different identified species in order to scientifically assess their potential. [ABSTRACT FROM AUTHOR]

In recent years, carbon quantum dots (CQDs) have garnered considerable attention as a promising material for biomedical applications because of their unique optical and biological properties. In this study, CQDs were derived from the leaves of Hibiscus rosa-sinensis Linn. via microwave-assisted technique and characterized using different techniques such as ultraviolet–visible, Fourier transform infrared, fluorescence spectrometry, X-ray diffraction, dynamic light scattering, transmission electron microscopy and energy-dispersive X-ray spectroscopy. Subsequently, their potential for biomedical applications was investigated through in vitro assays assessing scratch healing, anti-inflammatory, antibacterial, and cytotoxicity properties. It was found that the CQDs were fluorescent, polycrystalline, quasi-spherical, ~ 12 nm in size with presence of –OH and –COOH groups on their negatively charged surfaces, and demonstrated good anti-inflammatory by inhibiting protein denaturation, cyclooxygenase-2 and regulating inflammatory cytokines. The CQDs also exhibited antimicrobial activity against Klebsiella pneumoniae and Bacillus cereus, good biocompatibility, along with excellent promotion of cell proliferation in vitro, indicating their potential as a anti-inflammatory and wound healing material. The properties were more enhanced than their precursor, H. rosa-sinensis leaf extract. Hence, the CQDs synthesized from the leaves of H. rosa-sinensis can serve as a potential biomedical agent. [ABSTRACT FROM AUTHOR]

Lung cancer is a major public health issue and heavy burden in China and worldwide due to its high incidence and mortality without effective treatment. It's imperative to develop new treatments to overcome drug resistance. Natural products from food source, given their wide-ranging and long-term benefits, have been increasingly used in tumor prevention and treatment. This study revealed that Hibiscus manihot L. flower extract (HML) suppressed the proliferation and migration of A549 cells in a dose and time dependent manner and disrupting cell cycle progression. HML markedly enhanced the accumulation of ROS, stimulated the dissipation of mitochondrial membrane potential (MMP) and that facilitated mitophagy through the loss of mitochondrial function. In addition, HML induced apoptosis by activation of the PTEN-P53 pathway and inhibition of ATG5/7-dependent autophagy induced by PINK1-mediated mitophagy in A549 cells. Moreover, HML exert anticancer effects together with 5-FU through synergistic effect. Taken together, HML may serve as a potential tumor prevention and adjuvant treatment for its functional attributes. [ABSTRACT FROM AUTHOR]

Cancer, diabetes, and wounds are critical health challenges affecting millions of people worldwide. Cancer arises from the transformation of normal cells into tumor cells, leading to uncontrolled growth and potential spread to other parts of the body. Diabetes is a chronic metabolic disorder characterized by elevated blood sugar levels, and wounds can result from various injuries and diseases. In this study, we investigated the therapeutic potential of Hibiscus rosa-sinensis for its anticancer, antidiabetic, and in vitro wound healing activities. The anticancer activity of the ethanolic extract of H. rosa-sinensis was evaluated using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, which showed a concentration-dependent decrease in cell proliferation and growth. The extract exhibited promising anticancer potential, which could help control cancer progression. For the antidiabetic activity, we assessed a-amylase inhibition using a colorimetric method. The extract demonstrated substantial a-amylase inhibitory activity, comparable with the standard metformin. This indicates its potential in reducing postprandial blood sugar spikes in diabetic patients. In the in vitro wound healing activity, a scratch assay was performed to measure cell migration and growth. The results showed that H. rosa-sinensis extract promoted wound closure similar to the control. The extract's wound healing properties suggest its potential use in managing various types of wounds. Phytochemical analysis confirmed the presence of key bioactive compounds in both ethanolic and aqueous extracts of H. rosa-sinensis, supporting the observed therapeutic effects. In conclusion, H. rosa-sinensis holds promise as a potential source of novel therapeutic agent for cancer, diabetes, and wound management. The results from this study provide valuable insights into the plant's pharmacological activities and may pave the way for the development of new herbal-based treatments for these conditions. [ABSTRACT FROM AUTHOR]

Worm infections remain a significant health challenge globally, including in Indonesia, where they continue to contribute to a majority of health issues. Soil-transmitted helminths (STH), particularly intestinal nematode parasites, are predominant in causing these infections. Microscopic examination with 2% eosin staining is a conventional method for diagnosing worm infections. However, exploring alternative staining agents, such as anthocyanin-rich extracts from Hibiscus rosasinensis L. (hibiscus flower) and Hibiscus sabdariffa L. (roselle flower), presents a promising avenue. These plants possess anthocyanin compounds, resulting in a captivating red pigment and making them suitable for utilization as natural coloring agents. This study aimed to evaluate the efficacy of hibiscus and roselle infusions as alternative staining reagents for examining STH egg, compared to the standard 2% eosin staining method. Adopting a true experimental design, samples of STH worm egg suspensions were subjected to different concentrations of hibiscus and roselle infusions. The results revealed varying staining outcomes with different concentrations. However, a 1: 1 concentration ratio of hibiscus and roselle infusions demonstrated effective staining against STH worm eggs. In conclusion, this research underscores the comparative effectiveness of hibiscus and roselle infusions as alternative staining reagents for STH egg examination, with 2% eosin serving as the control. These findings hold potential implications for improving diagnostic techniques in managing worm infections. [ABSTRACT FROM AUTHOR]

The development of Supercritical Fluid Extraction (SFE) has opened the door to the harvesting of plants for a wide range of chemical compounds. This study distilled essential oil from hibiscus flowers utilizing the supercritical CO2 method. Different extraction parameters, including pressure (100-300 bar) and temperature (300-350 K), were studied to visualize how they affected oil recovery. Response surface analysis was used to fine-tune the extraction process. The chemical composition of the recovered oil was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). According to the findings, 13.11% per 80 g of dry flowers is the ideal oil extracted from Hibiscus flowers, using SFE at 200 bar pressure and 325 K extraction temperature. Six compounds were provisionally identified in the extracted oil from hibiscus flowers under optimum SFE conditions. [ABSTRACT FROM AUTHOR]

Nitrite is a vital preservative widely used in meat processing. Carcinogenic N-nitroso compounds discovered in processed meat products became serious impacts negatively affect meat industry. The present study provided a nitrite free fresh sausage with a novel method to substitute nitrite with a mixture consisted of (400 ppm nisin+25 ppm Zinc oxide nanoparticles+ 1% Hibiscus sabdariffa extract +1% chitosan) employing nisin (N) and Zinc oxide nanoparticles (ZON) as antimicrobials, chitosan (C) as antioxidant and H. sabdariffa extract (H) for color improving. In vitro antibacterial activity of nisin and ZON against Clostridium perfringens reference strain was estimated using agar well diffusion test. H. sabdariffa extract was evaluated for its phenolic and flavonoid contents as well as its phytochemical profile was analyzed using HPLC. Four variants of fresh sausage with different treatments were prepared: Nitrite (NT) treated, (N.C.H), (ZON.C.H) and (N.ZON.C.H), packed in polyethylene bags and kept in a refrigerator at 4 °C. Samples were examined to assess their pH, microbiological evaluation (aerobic plate, Enterobacteriaceae, yeast and mold counts), also, sensory assessment and shelf life were evaluated. Results exhibited that nisin and ZON have potent antibacterial activity against Cl. perfringens with inhibition zone of 30 mm and 15 mm compared to 30 mm of sodium nitrite. Hibiscus extract manifested a high phenolic content about 27.213 mg Gallic acid/g, 2.896 mg QE/g for flavonoids and about 19 main phenolic compounds were detected by HPLC. Results exhibited that N.ZON.C.H group samples showed the lowest pH values, APC, Enterobacteriaceae, yeast and mold counts among sausage variants. Regarding shelf life and sensory evaluation, as expected, N.ZON.C.H showed the highest sensory scores and shelf life (18 days) followed by N.C.H and ZON.C.H that remained accepted till the 15th and finally NT till the 12th day of cold storage. Results suggested that combination of nisin, ZON, chitosan and H. sabdariffa extract would be a promising new strategy to replace sodium nitrite and improve safety and quality of fresh sausage. [ABSTRACT FROM AUTHOR]

ABSTRACT Purpose: Hibiscus sabdariffa (HS) extract has several health benefits and anti-obesogenic effects. The aim of the present study was to assess whether the medicinal properties attributable to HS would prevent or mitigate bladder changes induced by obesity in an experimental model. Methods: Forty-eight male Wistar rats were submitted to one of four different dietary interventions (12 animals each): G1, standard diet and water (controls); G2, standard diet and HS tea; G3, a palatable high-fat diet and water; and G4, high-fat diet diet and HS tea. The animals were monitored for body weight, feed, and water and tea intake, according to the allocated group. After 16 weeks, the animals were euthanized, and the levels of creatinine, inflammatory cytokines, testosterone, cholesterol, triglycerides, and electrolytes were evaluated. In addition, histopathological analysis of the animals’ bladder was performed. Results: Groups receiving HS (G2 and G4) showed decreased levels of the pro-inflammatory cytokine interleukin-1α. HS tea was able to reduce low-density lipoprotein and triglyceride levels in the G2 group compared to other groups. Only in the G3 there was a significant increase in the body weight when it was compared the 12th and 16th weeks. Leptin was shown to be elevated in the groups that received a high-fat diet. There was a significant decrease in the muscle fibers thickness and in the total collagen count in G4 bladder when compared with G1 and G3. Conclusions: HS has an anti-inflammatory role, can reverse hyperlipidemia in rats, and reduced deleterious effects of obesity on these animals’ bladder.

Comparative analysis of the microbial properties of Hibiscus sabdariffa calyces and leaves (Red variety) was subjected to different thermal drying methods: electric oven, infrared oven, and sun drying was conducted using standard analytical and molecular techniques. The study revealed significant differences (p

Summary. In Spring 2024, a symptomatic plant of China rose (Hibiscus rosa-sinensis) showing leaf yellowing and deformation, located in the province of Naples (South Ita- ly), was re-sampled for further investigations. Total RNAs were purified from leaves and subjected to Illumina HTS analysis. Results confirmed that the plant was infected by hibiscus chlorotic ringspot virus (HCRSV), citrus exocortis viroid (CEVd), and cit- rus viroid VI (CVd-VI). The two viroids had not been previously detected in China rose. HTS results were confirmed by RT-PCR in the re-sampled plant, and in three nearby China rose plants with the same symptoms, using specific primer pairs for the three pathogens, followed by Sanger sequencing and BLAST analysis of the sequences. Different results obtained can be due to differing sensitivity and specificity in HTS- based detection of plant viruses. This is the first report of multiple infections by HCRS, CEVd and CVd-VI in China rose, which is also a new host for both of the viroids. [ABSTRACT FROM AUTHOR]

The Hibiscus manihot L. (HML) Medic, an edible hibiscus of the Malvaceae family, is abundant with flavonoids. The study investigated how Rhizopus-arrhizus-31-assisted pretreatment affects the extraction and bioactivity of flavonoids from HML. The fiber structure of the fermented flavonoid sample (RFF) appears looser, more porous, and more disordered than the unfermented flavonoid sample (RUF). RFF demonstrates milder conditions and yields higher extraction rates. According to the Box–Behnken response surface optimization experiment, the optimal conditions for RFF include a material–liquid ratio of 1:41 g/mL, a 2 h extraction time, a 57% ethanol concentration, and an extraction temperature of 800 °C, resulting in a 3.69% extraction yield, which is 39.25% higher than that of RUF. Additionally, RFF exhibits greater activity than RUF in the radical-scavenging system. The IC50 values for DPPH, OH, and ABTS radicals are 83.43 μg/mL and 82.62 μg/mL, 208.38 μg/mL and 175.99 μg/mL, and 108.59 μg/mL and 75.39 μg/mL for RUF and RFF, respectively. UPLC-QTOF-MS analysis of the active components in the HML flavonoid sample revealed significant differences in the chromatograms of RUF and RFF, indicating that biofermentation led to substantial changes in composition and content from HML. [ABSTRACT FROM AUTHOR]

The chronic metabolic disease known as diabetes mellitus causes hyperglycemia in the body. Antioxidant and antidiabetic qualities are well-known benefits of Hibiscus rosa sinensis (HRS). In this work, diabetic Wistar albino rats were used to assess the antidiabetic properties of HRS flower extract. A total of 18 animals were taken and divided into three groups (n = 6) - (Group 1): Normal control group, (Group 2): Diabetic control group, (Group 3): Diabetic group treated with a 125 mg/kg dose of HRS flower extract. Group 2 animals showed a progressive decrease in body weight, while Group 1 animals showed a considerable gain in body weight. After overcoming the weight loss, Group 3 animals also showed an increase in body weight that was similar to Group 1. Group 2 animals had blood glucose levels higher than 400 mg/dL, but Group 1 animals had blood glucose levels below 200 mg/dL throughout the experiment. Group 3 animals first had glucose levels higher than 350 mg/dL and then lower than 200 mg/dL, comparable to Group 1 animals. Upon histological examination, the pancreatic islets of Group 2 animals showed vacuolation, necrosis, and degeneration. The animals in Group 3 displayed regenerated islets of Langerhans and enhanced pancreatic anatomy. The animals in Group 3 also returned to normal in terms of body weight and blood glucose levels, similar to those in Group 1. These findings show that Hibiscus rosa sinensis has potential as an alternative diabetic treatment; further research is needed to fully understand its modes of action and long-term effects. [ABSTRACT FROM AUTHOR]

This study investigated the physicochemical properties of Hibiscus congestiflorus leaf powder (HCLP) and its application, as a foaming agent, in the foam-mat drying of cowpea paste using glyceryl monostearate as reference. The protein, fat, fiber, ash, K, Fe, flavonoid, and alkaloid contents of HCLP were 21.09 ± 0.20 g/100 g, 2.37 ± 0.10 g/100 g, 13.42 ± 0.04 g/100 g, 13.72 ± 0.20 g/100 g, 146.00 ± 1.42 mg/100 g, 46.65 ± 0.98 mg/100 g, 94.10 ± 1.21 mg/100 g, and 48.00 ± 0.43 mg/100 g, respectively, suggesting its high nutrient density. There was no significant difference (p >.05) between the cowpea powder foam-mat-dried with HCLP (CPHC) and cowpea powder foam-mat-dried with glyceryl monostearate (CPGM) in foam density and solubility. CPGM had significantly (p <.05) higher bulk density, water absorption, swelling capacity, L* value, and energy value while CPHC had higher (p <.05) dispersibility and proximate composition. HCLP had excellent physicochemical properties that could be exploited in foam-mat drying. [ABSTRACT FROM AUTHOR]

It is known that endophytes can produce the same or similar secondary metabolites as their host plants. Medicinal plants have been proven to be a source of microorganisms that can make certain metabolites. Our previous research succeeded in isolating the endophytic bacteria Staphylococcus warneri strain 25 from waru leaves (Hibiscus tiliaceus), which had antioxidant and antibacterial activity and contained saponins and alkaloids. In this study, alkaloids were extracted from the endophytic bacteria found in waru leaves. Subsequently, their bioactivity was assessed for antibacterial properties against Escherichia coli, Salmonella typhi, and Staphylococcus aureus using the well diffusion method. Additionally, their antioxidant activity was evaluated through the DPPH method, with gallic acid serving as the standard. A cyclic amine alkaloid compound was isolated, exhibiting a Minimum Inhibitory Concentration (MIC) of 23 ppm against E. coli, S. typhi, and S. aureus bacteria, accompanied by inhibitory zones measuring 7 mm, 8 mm, and 6 mm, respectively. Additionally, the compound had very strong antioxidant activity, with an IC50 value of 50.11 ppm. [ABSTRACT FROM AUTHOR]

This study examined the effects of adding herbs, spices, and fruits into fermented Olympus Mountain tea (Sideritis scardica) kombucha using thyme honey as a sweetener. This study evaluated how these additions affected the tea's physical, chemical, and functional characteristics. Two different enrichments were proposed: a "Golden Mountain tea and honey Kombucha" (KG) with fresh ginger, turmeric powder, and lemon zest and juice and a "Red Mountain tea and honey Kombucha" (KR) with dried hibiscus calyces, rose petals, and lavender blossoms. In KR, the levels of vitamin C increased from 33.2 ± 2.7 to 48.4 ± 4.5. Additionally, the levels of calcium increased from 31.0 ± 1.2 to 55.7 ± 1.2, while the levels of potassium practically doubled from 64.7 ± 0.6 to 115.7 ± 2.5. An increased potassium concentration was observed in KG, and ionic iron was found for the first time after both enrichments. The total phenolic and flavonoid contents, along with antioxidant capacity, as assessed by the ABTS and DPPH methods, were found to be substantially enhanced in KR. In KG, the total phenolic content increased, together with antioxidant activity, as assessed by ABTS. Enrichment with hibiscus calyces, rose petals, and lavender blossoms significantly increased inhibitory effects against α-amylase, α-glucosidase, acetylcholinesterase, and butyrylcholinesterase. On the other hand, enrichment with ginger, turmeric, and lemon zest and juice decreased inhibitory effects against α-glucosidase and increased those against α-amylase, acetylcholinesterase, and butyrylcholinesterase. KR had the strongest enzyme-inhibiting activity, with its α-glucosidase-inhibiting activity increased by approximately 18 times. Therefore, enrichment with selected herbs, spices, and fruits can transform fermented Olympus Mountain tea kombucha sweetened with honey into a novel beverage with enhanced functional properties. [ABSTRACT FROM AUTHOR]

Tisane is a fruit or herbal infusion, commonly referred to as herbal tea. These products are consumed as part of a balanced diet, which is closely related to the trend of a healthier lifestyle. In this work, tisanes prepared from rosehip (R), and herbal mixtures containing rosehip/hibiscus flowers (R/H) and rosehip/hibiscus flowers/saffron (R/H/S) were studied. Rosehip was dried by the convective drying method at 40, 50 and 60 °C. Total phenolic content (TPC), total flavonoid content (TFC), total flavonol content (TFlC), total anthocyanin content (TAC), antioxidant properties (DPPH∙ and ABTS∙+ assays) and in vitro inhibitory potential toward α-amylase of tisanes were examined. The highest TPC (based on dry weight (dw)) was measured in tisane obtained from rosehip dried at 60 °C (37.84 mg GAE/g dw). Tisanes prepared from a R/H/S mixture had the highest values of TFC (4.66–6.13 mg QUE/g dw), TFlC (2.67–3.98 mg QUE/g dw) and TAC (1.35–2.27 mg Cy 3-glc/g dw). The highest DPPH∙ scavenging activity (53.42 mg TE/g dw) was measured in rosehip (dried at 60 °C) tisane, whereas tisane prepared from a rosehip (dried at 60 °C)/hibiscus mixture expressed the best ABTS∙+ scavenging activity (107.44 mg TE/g dw). All tisane samples expressed high inhibitory potential toward α-amylase, with the highest activity of 85.03% and 89.90%, measured for tisanes prepared from rosehip/hibiscus flowers mixture (rosehip dried at 50 and 60 °C, respectively). [ABSTRACT FROM AUTHOR]

The dried flowers of Hibiscus sabdariffa (HS), available worldwide, have various applications in both non-medicinal and medicinal fields. The growing global interest in the health benefits of HS is linked to its potential prevention or management of non-communicable diseases. The aim of this research was to find the optimal extraction method that ensures the maximum yield of multiple beneficial bioactive components, such as polyphenols, anthocyanins, vitamin C, β-carotene, antioxidant activity, free radical scavenging activity DPPH and ferric reducing antioxidant power (FRAP). To this end, stirring, pulsed electric field, and ultrasound-assisted extraction were evaluated, either alone or in combination. Under optimized extraction conditions, the obtained extract exhibited an elevated total polyphenol content (37.82 mg of gallic acid equivalents/g dry weight (dw)), total anthocyanin content (610.42 μg of cyanidin equivalents/g dw), total carotenoids content (921.84 μg of β-carotene equivalents/g dw), and ascorbic acid content (507.44 mg/100 g dw). Remarkably, the extracts exhibited strong antioxidant properties (487.51 μmol of ascorbic acid equivalents (AAE)/g dw and 243.42 μmol AAE/g dw as evidenced by FRAP and DPPH assays, respectively). This research advances the parameters that should be employed to produce the optimal and nutritionally enhanced HS flower extracts, that can be used in the commercial sector. [ABSTRACT FROM AUTHOR]

This document explores the potential of natural compounds found in Hibiscus sabdariffa as adjuncts in the treatment of hypertension. The study focuses on the inhibitory effects of Hibiscus sabdariffa on angiotensin-converting enzyme (ACE) and its potential synergistic interactions with other compounds. The researchers used in vitro and in silico methods to evaluate the inhibitory activity of different chromatographic collections of Hibiscus sabdariffa extract. The results suggest that certain collections exhibited significant ACE inhibition, comparable to the activity of the antihypertensive drug captopril. The study also conducted Nuclear Magnetic Resonance analysis to further analyze the compounds present in the chromatographic collections. Overall, this research provides insights into the potential of Hibiscus sabdariffa as a natural adjunct in antihypertensive treatment. [Extracted from the article]

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Hibiscus syriacus L. is a renowned ornamental plant. We constructed 95 chloroplast genomes of H. syriacus L. cultivars using a short-read sequencing platform (Illumina) and a long-read sequencing platform (Oxford Nanopore Technology). The following genome assembly, we delineate quadripartite structures encompassing large single-copy, small single-copy, and inverted repeat (IRa and IRb) regions, from 160,231 bp to 161,041 bp. Our comprehensive analyses confirmed the presence of 79 protein-coding genes, 30 tRNA genes, and 4 rRNA genes in the pan-chloroplast genome, consistent with prior research on the H. syriacus chloroplast genome. Subsequent pangenome analysis unveiled widespread genome sequence conservation alongside unique cultivar-specific variant patterns consisting of 193 single-nucleotide polymorphisms and 61 insertions or deletions. The region containing intra-species variant patterns, as identified in this study, has the potential to develop accession-specific molecular markers, enhancing precision in cultivar classification. These findings are anticipated to drive advancements in breeding strategies, augment biodiversity, and unlock the agricultural potential inherent in H. syriacus. [ABSTRACT FROM AUTHOR]

Hibiscus is not native to Colombia but well suited to its arid soil and dry climates. A single hibiscus plant from Risaralda, showing black spots on upper and lower sides of its leaves, was collected for virome analysis using meta-transcriptomic high-throughput sequencing technology. Bioinformatic analysis identified 12.5% of the total reads in the Ribo-Zero cDNA library which mapped to viral genomes. BLAST searches revealed the presence of carlavirus, potexvirus, and of known members of the genera Betacarmovirus, Cilevirus, Nepovirus, and Tobamovirus in the sample; confirmed by RT-PCR with virus-specific primers followed by amplicon sequencing. Furthermore, in silico analysis suggested the possibility of a novel soymovirus, and a new hibiscus strain of citrus leprosis virus C2 in the mixed infection. Both RNA dependent RNA polymerase and coat protein gene sequences of the potex and carla viruses shared less than 72% nucleotide and 80% amino acid identities with any alphaflexi- and betaflexi-virus sequences available in GenBank, identifying three novel carlavirus and one potexvirus species in the Hibiscus rosa-sinensis plant. The detection of physalis vein necrosis nepovirus and passion fruit green spot cilevirus in hibiscus are also new reports from Colombia. Overall, the meta-transcriptome analysis identified the complex virome associated with the black spot symptoms on hibiscus leaves and demonstrated the diversity of virus genera tolerated in the mixed infection of a single H. rosa-sinensis plant. [ABSTRACT FROM AUTHOR]

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