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The detection of high‐risk human papillomaviruses (HPVs) is crucial for early screening and preventing cervical cancer. However, the substantial workload in high‐level hospitals or the limited resources in primary‐level hospitals hinder widespread testing. To address this issue, we explored a sample‐to‐answer genotyping system and assessed its performance by comparing it with the traditional real‐time polymerase chain reaction (PCR) method conducted manually. Samples randomly selected from those undergoing routine real‐time PCR detection were re‐analyzed using the fully automatic GenPlex® system. This system identifies 24 types of HPV through a combination of ordinary PCR and microarray‐based reverse hybridization. Inconsistent results were confirmed by repeated testing with both methods, and the κ concordance test was employed to evaluate differences between the two methods. A total of 365 samples were randomly selected from 7259 women. According to real‐time PCR results, 76 were high‐risk HPV negative, and 289 were positive. The GenPlex® system achieved a κ value greater than 0.9 (ranging from 0.920 to 1.000, p < 0.0001) for 14 types of high‐risk HPV, except HPV 51 (κ = 0.697, p < 0.0001). However, the inconsistent results in high‐risk HPV 51 were revealed to be false positive in real‐time PCR by other method. When counting by samples without discriminating the high‐risk HPV type, the results of both methods were entirely consistent (κ = 1.000, p < 0.0001). Notably, the GenPlex® system identified more positive cases, with 73 having an HPV type not covered by real‐time PCR, and 20 potentially due to low DNA concentration undetectable by the latter. Compared with the routinely used real‐time PCR assay, the GenPlex® system demonstrated high consistency. Importantly, the system's advantages in automatic operation and a sealed lab‐on‐chip format respectively reduce manual work and prevent aerosol pollution. For widespread use of GenPlex® system, formal clinical validation following international criteria should be warranted. [ABSTRACT FROM AUTHOR]

The prerequisite for flexible magnetic electronic devices is the knowledge of the preparation technology of flexible magnetic films and the evolution of the film properties under strain. In this work, CoFeB amorphous ferromagnetic films with stripe domains were prepared on flexible polyimide (PI) substrates by oblique sputtering. The results show that oblique sputtering induces the formation of columnar crystal structure in CoFeB films, which increases the perpendicular magnetic anisotropy of the films, thus leading to the appearance of stripe magnetic domain structures. On this basis, the CoFeB films with stripe domains were processed on a microscopic scale to investigate the size effect and strain regulation on the microscopic domain structure of the magnetic films. The characterization of the magnetic domain structure shows that the stripe domain contrast is reduced by the striped structure prepared by lithography. The triangular, circular and ring patterns deflect the alignment of the stripe domain to different degrees. The experimental results show that the deflection of the stripe domains is caused by the anisotropy of the shapes produced by the different patterns and that the size of the microstructure needs to be close to the period of the stripe domains for the size effect to be significant. In addition, the strain-induced magnetoelastic anisotropy effectively rotates the orientation of the stripe domains, and the variation in domain contrast demonstrates that tensile/compressive strains vary the magnitude of the out-of-plane stray field of the film. Our results provide some insight into the modulation of the physical properties of flexible magnetic films. [ABSTRACT FROM AUTHOR]

Using literature review, questionnaire and interviews, the key time sequence node data for the growth of 175 Olympic champions were analyzed. It is found that: (I) The average training time to become Olympic champions is 13.85 years. In the time sequence of the entire growth stage of Olympic champions, the youth training period is the longest (average 5.66 years) and has great gender and event group differences. There is an inverse relationship between the training start age and the training start period. As long as adolescent training is within a reasonable age range, it will be "the same" success sooner or later. but premature specialized training may not be conducive to the rapid development of the elite level in adulthood. (2) In comparison, the phenomenon of "male long female short" for the time sequence of Olympic champions' growth is more common, and the characteristics of physiological development of "male late, female early" are not the only basis for the start of training. (3) The age of the start of training is more reasonable according to the characteristics of the sports. It is recommended to divide it into 3 age groups of young, middle and old. The athlete of individual event that compete against each other across the net which are known to be China's dominant event are the typical "start training at a very young age and need to train carefully" and become a talent late, but not suitable for most events of strength and condition. Male athletes in the endurance event group may start late and become quick. [ABSTRACT FROM AUTHOR]

Background: This study was initiated to investigate the difference in HER2 status between tumor tissue and circulating tumor cells (CTCs), as well as the predictive value of CTC HER2 status for predicting the outcomes of anti-HER2 therapy in histologically HER2-positive metastatic breast cancer (MBC) patients.Methods: HER2 expression on CTCs was detected using a CellSearch system within 7 days before a new line of anti-HER2 therapy was begun. According to the criterion proposed in our previous report, patients were defined as CTC HER2-positive or -negative. After close follow-up, the correlation between CTC HER2 status and the outcome of the treatment was evaluated by statistical analysis.Results: CTCs were detected in 57.4 % (58/101) of the patients. Notably, 62.1 % (36/58) of these patients had an inconsistent HER2 status between their tissue and CTCs. The discordant rate may correlate with the time interval between histological and CTC HER2 testing and is more likely to occur in the subgroup of patients with an interval of > 1 year than in those with an interval < 1 year (70.7 % vs. 41.2 %, P = 0.043). For PFS, positive HER2 status on CTCs was shown to be a valuable predictor, both in univariate (HR = 0.321, 95%CI, 0.156-0.62, P = 0.0011) and multivariate (HR = 0.383, 95%CI, 0.166-0.831, P = 0.019) Cox regression analysis. Meanwhile, Kaplan-Meier survival curves revealed that the median PFS of CTC HER2-positive patients was significantly longer than CTC HER2-negative ones (8.5 vs. 3.5 months, P < 0.001).Conclusions: HER2 status on CTCs was different from that of tumor tissues and predicted a different outcome of the patients' anti-HER2 therapy. This difference may be correlated with the time interval between tissue and CTC HER2 testing, indicating the necessity of real-time HER2 analysis for histologically HER2-positive MBC patients. [ABSTRACT FROM AUTHOR]