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36 results on '"Ha, Zhuo"'

9. Interaction of species A rotavirus VP4 with the cellular proteins vimentin and actin related protein 2 discovered by a proximity interactome assay

Author

Hao, Pengfei, primary, Qu, Qiaoqiao, additional, Pang, Zhaoxia, additional, Li, Letian, additional, Du, Shouwen, additional, Shang, Limin, additional, Jin, Chaozhi, additional, Xu, Wang, additional, Ha, Zhuo, additional, Jiang, Yuhang, additional, Chen, Jing, additional, Gao, Zihan, additional, Jin, Ningyi, additional, Wang, Jian, additional, and Li, Chang, additional

Published
2023
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13. Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Author

Zhao, Guanyu, primary, Gao, Yan, additional, Shi, Ning, additional, Zhang, Shiheng, additional, Xiao, Pengpeng, additional, Zhang, Jiaqi, additional, Xie, Changzhan, additional, Ha, Zhuo, additional, Feng, Sheng, additional, Li, Chenghui, additional, Zhang, Xuancheng, additional, Xie, Yubiao, additional, Yu, Ning, additional, Zhang, He, additional, Bi, Junlong, additional, and Jin, Ningyi, additional

Published
2023
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15. Immunological evaluation of recombination PRRSV GP3 and GP5 DNA vaccines in vivo

Author

Zhao, Guanyu, primary, Zhang, Jiaqi, additional, Sun, Wenchao, additional, Xie, Changzhan, additional, Zhang, He, additional, Gao, Yan, additional, Wen, Shubo, additional, Ha, Zhuo, additional, Nan, Fulong, additional, Zhu, Xiangyu, additional, Feng, Sheng, additional, Cao, Xinyu, additional, Zhang, Ying, additional, Zhu, Yanzhu, additional, Jin, Ningyi, additional, and Lu, Huijun, additional

Published
2022
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16. Establishment of a reverse transcription real-time quantitative PCR method for Getah virus detection and its application for epidemiological investigation in Shandong, China

Author

Cao, Xinyu, primary, Qiu, Xiangshu, additional, Shi, Ning, additional, Ha, Zhuo, additional, Zhang, He, additional, Xie, Yubiao, additional, Wang, Peng, additional, Zhu, Xiangyu, additional, Zhao, Wenxin, additional, Zhao, Guanyu, additional, Jin, Ningyi, additional, and Lu, Huijun, additional

Published
2022
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17. Toll-like receptor 2 signaling pathway activation contributes to a highly efficient inflammatory response in Japanese encephalitis virus-infected mouse microglial cells by proteomics

Author

Zhao, Guanyu, primary, Gao, Yan, additional, Zhang, Jiaqi, additional, Zhang, He, additional, Xie, Changzhan, additional, Nan, Fulong, additional, Feng, Sheng, additional, Ha, Zhuo, additional, Li, Chenghui, additional, Zhu, Xiangyu, additional, Li, Zhuoxin, additional, Zhang, Ping, additional, Zhang, Ying, additional, Lu, Huijun, additional, and Jin, Ningyi, additional

Published
2022
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22. Lentogenic NDV V protein inhibits IFN responses and represses cell apoptosis

Author

Nan, Fu Long, primary, Zhang, He, additional, Nan, Wen Long, additional, Xie, Chang Zhan, additional, Ha, Zhuo, additional, Chen, Xing, additional, Xu, Xiao Hong, additional, Qian, Jing, additional, Qiu, Xu Sheng, additional, Ge, Jin Ying, additional, Bu, Zhi Gao, additional, Zhang, Ying, additional, Lu, Hui Jun, additional, and Jin, Ning Yi, additional

Published
2021
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23. Additional file 1 of Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm

Author

Zhang, Jinyong, Li, Chenghui, Meng, Yuan, Xie, Yubiao, Shi, Ning, Zhang, He, Yu, Chengdong, Nan, Fulong, Xie, Changzhan, Ha, Zhuo, Han, Jicheng, Li, Zhuoxin, Li, Qiuxuan, Wang, Peng, Gao, Xu, Jin, Ningyi, and Lu, Huijun

Abstract

Additional file 1: Table S1. Primers detection of SVV by qRT-PCR. Table S2. Primers detection of SVV.

Published
2021
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24. Additional file 3 of Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm

Author

Zhang, Jinyong, Li, Chenghui, Meng, Yuan, Xie, Yubiao, Shi, Ning, Zhang, He, Yu, Chengdong, Nan, Fulong, Xie, Changzhan, Ha, Zhuo, Han, Jicheng, Li, Zhuoxin, Li, Qiuxuan, Wang, Peng, Gao, Xu, Jin, Ningyi, and Lu, Huijun

Abstract

Additional file 3: Fig. S2. Detection of SVV in Culicoides samples. 4/10 of the groups were positive, for group 3, 4, 5, 6 (A); the results of separate sample detection showed that sample 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 25, 30 was positive (B). Table S3. Results of SVV were detected in mosquitoes and Culicoides.

Published
2021
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25. Pathogenicity of Seneca Valley virus in Pigs and Detection in Culicoides from an Infected Pig Farm

Author

Zhang, Jinyong, primary, Li, Chenghui, additional, Meng, Yuan, additional, Xie, Yubiao, additional, Shi, Ning, additional, Zhang, He, additional, Yu, Chengdong, additional, Nan, Fulong, additional, Xie, Changzhan, additional, Ha, Zhuo, additional, Han, Jicheng, additional, Li, Zhuoxin, additional, Li, Qiuxuan, additional, Wang, Peng, additional, Gao, Xu, additional, Jin, Ningyi, additional, and Lu, Huijun, additional

Published
2021
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26. Newcastle Disease Virus Inhibits the Proliferation of T Cells Induced by Dendritic Cells In Vitro and In Vivo

Author

Nan, Fu Long, primary, Zheng, Wei, additional, Nan, Wen Long, additional, Yu, Tong, additional, Xie, Chang Zhan, additional, Zhang, He, additional, Xu, Xiao Hong, additional, Li, Cheng Hui, additional, Ha, Zhuo, additional, Zhang, Jin Yong, additional, Zhuang, Xin Yu, additional, Han, Ji Cheng, additional, Wang, Wei, additional, Qian, Jing, additional, Zhao, Guan Yu, additional, Li, Zhuo Xin, additional, Ge, Jin Ying, additional, Bu, Zhi Gao, additional, Zhang, Ying, additional, Lu, Hui Jun, additional, and Jin, Ning Yi, additional

Published
2021
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27. Prevalence, pathogenesis, and evolution of porcine circovirus type 3 in China from 2016 to 2019

Author

Ha, Zhuo, primary, Li, Jinfeng, additional, Xie, Changzhan, additional, Yu, Chengdong, additional, Hao, Pengfei, additional, Zhang, Ying, additional, Xu, Wang, additional, Nan, Fulong, additional, Xie, Yubiao, additional, Li, Yanwei, additional, Rong, Fulong, additional, Wang, Guanyu, additional, Guo, Yingcheng, additional, Lu, Huijun, additional, and Jin, Ningyi, additional

Published
2020
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29. Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1-7-4 viruses) in China

Author

Xie, Chang-Zhan, primary, Ha, Zhuo, additional, Zhang, Han, additional, Zhang, Ying, additional, Xie, Yu-Biao, additional, Zhang, He, additional, Nan, Fu-Long, additional, Wang, Zheng, additional, Zhang, Ping, additional, Xu, Wang, additional, Han, Ji-Cheng, additional, Wen, Shu-Bo, additional, Lu, Hui-Jun, additional, and Jin, Ning-Yi, additional

Published
2020
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30. First detection and genomic characterization of porcine circovirus 3 in mosquitoes from pig farms in China

Author

Ha, Zhuo, primary, Li, Jin-Feng, additional, Xie, Chang-Zhan, additional, Li, Cheng-Hui, additional, Zhou, Hong-Ning, additional, Zhang, Ying, additional, Hao, Peng-Fei, additional, Nan, Fu-Long, additional, Zhang, Jin-Yong, additional, Han, Ji-Cheng, additional, Zhang, He, additional, Zhuang, Xin-Yu, additional, Guo, Ying-Cheng, additional, Lu, Hui-Jun, additional, and Jin, Ning-Yi, additional

Published
2020
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31. Construction and immunological evaluation of recombinant Newcastle disease virus vaccines expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP3/GP5 proteins in pigs

Author

Zhang, He, primary, Nan, Fulong, additional, Li, Zhuoxin, additional, Zhao, Guanyu, additional, Xie, Changzhan, additional, Ha, Zhuo, additional, Zhang, Jinyong, additional, Han, Jicheng, additional, Xiao, Pengpeng, additional, Zhuang, Xinyu, additional, Wang, Wei, additional, Ge, Jinying, additional, Tian, Mingyao, additional, Lu, Huijun, additional, Bu, Zhigao, additional, and Jin, Ningyi, additional

Published
2019
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32. Construction and immunological evaluation of recombinant adenovirus vaccines co-expressing GP3 and GP5 of EU-type porcine reproductive and respiratory syndrome virus in pigs

Author

XIE, Changzhan, primary, HA, Zhuo, additional, SUN, Wenchao, additional, NAN, Fulong, additional, ZHANG, Ping, additional, HAN, Jicheng, additional, ZHAO, Guanyu, additional, ZHANG, He, additional, ZHUANG, Xinyu, additional, LU, Huijun, additional, and JIN, Ningyi, additional

Published
2019
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33. Prokaryotic expression of VP3 gene of infectious pancreas necrosis virus and antigenicity of expressed product

Author

Ge JunWei, Li YiJing, Ha Zhuo, Liu Min, Zhao Yongxin, Zhao LiLi, Liu WeiWei, and Qiao XinYuan

Subjects
Antiserum, Antigenicity, Viral inner capsid, viruses, virus diseases, biochemical phenomena, metabolism, and nutrition, Management, Monitoring, Policy and Law, Aquatic Science, Biology, Virology, Molecular biology, Fusion protein, Virus, law.invention, law, Gene expression, Recombinant DNA, Infectious pancreatic necrosis, Ecology, Evolution, Behavior and Systematics
Abstract

Infectious pancreatic necrosis(IPN) virus,the etiologic agent of infectious pancreatic necrosis in salmonid fish,causes significant losses to the aquaculture industry.The gene for the viral inner capsid protein(VP3) was amplified by RT-PCR method from IPNV,and cloned into pET30b vector.The expression of recombinant plasmid pET30b-VP3 in E.coli BL21(DE3) was induced and detected by SDS-PAGE analysis.The predicted molecular weight for unmodified r-trunc VP3 was approximately 30 ku and this was found to be the case for E.coli protein.The amount of expression made up 30 percent of the bacteria protein total expression by thin layer scanning analysis.The results showed that the VP3 gene of IPNV can express successfully in E.coli BL21.The fusion protein was purified with ProBondTM resin from the suspension centrifuged and the antisera against VP3 protein was produced.The pET30b-VP3 fusion protein can be recognized by the positive serum of IPNV by Western-blotting analysis.The prepared antisera reacted specifically with IPNV antigen by indirect ELISA.The antisera against VP3 protein had OD values at least twice that obtained for the negative control serum at a dilution of 1 :25 600.The results showed that the expressed VP3 protein was immunogenical and antigenical which is the same as the natural IPNV VP3 protein.In this experiment the IPNV VP3 protein was expressed successfully by using prokaryotic expression system.The expressed fusion protein was active and the antisera against VP3 protein were produced.

Published
2010
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35. Characterization and pathogenesis of swine influenza virus isolate.

Author

HA Zhuo, Peng-chao, ZHANG, Ll Qian, Xiao-xiao, SHI, Bai-zhong, RAO, Yong-jie, LIU, and Hui-li, LIU

Abstract

The article focuses on the Swine influenza virus, a virus that infects swine that has been isolated and its pathogenesis along with its characterization. It explains the procedure to characterize the Swine flu virus by analysis of receptor sites, a binding site. It mentions that main amino acid indicates the sensitivity of virus towards drugs zanamivir and amantadine. It also states that the viral strain did not caused disease in chickens but was pathogenic to mice.

Published
2013

36. [High-level expression and antimicrobial activity of recombinant N-terminal porcine lactoferrin].

Author

Ha Z, Zhao L, Yu X, Zong X, Mao Y, Zhang J, Li Y, Ge J, Qiao X, and Tang L

Subjects
Amino Acid Sequence, Animals, Anti-Infective Agents metabolism, Base Sequence, Escherichia coli genetics, Escherichia coli metabolism, Genetic Vectors genetics, Lactoferrin genetics, Molecular Sequence Data, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics, Swine, Anti-Infective Agents pharmacology, Lactoferrin biosynthesis, Lactoferrin pharmacology, Recombinant Fusion Proteins pharmacology
Abstract

Lactoferrin in milk is a multifunctional protein. In addition, lactoferrin has antiviral, antifungal and antiparasitic activity. In this study, the N-terminus from porcine lactoferrin (PLF-N) was designed to express the antimicrobial action of recombinant porcine lactoferrin. We cloned a 1077 bp fragment of the PLF gene from mammary gland tissue of the lactating sow at the third day. Comparing nucleotide sequence with four strains of PLF gene published on GenBank, the homology was more than 99%. With the reference template of the cloned fragment of PLF-N and optimizing codon bias, we synthesized the gene of N-terminus encoding porcine lactoferrin (PLF-NS). The high expression gene of PLF-NS was cloned into the fusion expression vector pET30b and expressed in E. coli BL21 (DE3). After induced with Isopropyl beta-D-1-Thiogalactopyranoside (IPTG), the target fusion protein was successfully expressed and identified in inclusion bodies by SDS-PAGE and Western blotting. The protein had a molecular weight of 42 kDa and accounted for 32% of the total cellular protein. After purification and renaturation, the purity of the expressed protein was 98%. The expressed PLF-NS protein showed obviously antibacterial activity. This method provides an excellent way for high expression of antimicrobial proteins when optimizing codon bias.

Published
2010

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