14 results on '"Hai, Guangfan"'
Search Results
2. Anti-Hepatoma Activity of a Novel Compound Glaucocalyxin H In Vivo and In Vitro
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Hai, Guangfan, Zhang, Chong, Jia, Yanlong, Bai, Suping, Han, Jinfen, Guo, Lanqing, Cui, Taizhen, Niu, Bingxuan, Huang, Feng, and Song, Yu
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- 2015
- Full Text
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3. The Two‐Steps Reaction Fluorescent Probe for the Selective Detection of Cysteine and Its Applications**
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Liu, Xueliang, primary, Wei, Haiyuan, additional, Yan, Mengdi, additional, Hai, Guangfan, additional, Li, Qin, additional, Yan, Ruifang, additional, Zhao, Shan, additional, Zhao, Xiaoxia, additional, Sun, Xiaoqing, additional, and Zhang, Tao, additional
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- 2022
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4. Network pharmacology study on the active components of Pterocypsela elata and the mechanism of their effect against cerebral ischemia
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Yunjuan Jiao, Fulin Yan, Hai Guangfan, Yansheng Feng, Bingxuan Niu, Chunyan Li, Hui Zhang, and Yu Song
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0301 basic medicine ,Pharmacology ,Pterocypsela elata ,Cerebral infarction ,Mechanism (biology) ,Active components ,Ischemia ,Pharmaceutical Science ,Computational biology ,Biology ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Network pharmacology ,Drug Discovery ,medicine ,DrugBank ,Ribosyldihydronicotinamide dehydrogenase - Abstract
Objective The aim of this study was to identify the active anti-ischemic components of Pterocypsela elata (P. elata) using a network pharmacology approach to construct an effective component anti-cerebral ischemic target network and systematically analyze this medicinal material. Methods Pharmacological studies have shown that P. elata has an obvious effect against cerebral ischemia. To identify the potential targets, 14 components of P. elata were docked to each structural element of the targets in the DRAR-CPI database by reverse docking technology. We then compared the identified potential targets with FDA-approved targets for stroke/cerebral infarction treatment in the DrugBank database and identified the active components of P. elata and their potential targets for stroke/cerebral infarction treatment. The active component-target networks were constructed using Cytoscape 3.5.1 software. The target protein-protein interactions were analyzed using the STRING database. KEGG pathway analysis and gene ontology (GO) enrichment analysis were performed through the Database for Annotation, Visualization and Integrated Discovery (DAVID). Results There were 14 active components identified from P. elata and 21 potential targets identified for cerebral ischemia treatment, including carbonic anhydrase 2, ribosyldihydronicotinamide dehydrogenase, cholinesterase, and glutathione S-transferase P. The main involved pathways include metabolic pathways, complement and coagulation cascades and steroid hormone biosynthesis. Conclusion Through a network pharmacology approach, we predicted the active components of P. elata and their potential targets for cerebral ischemia treatment. Our results provide new perspectives and clues for further studies on the anti-cerebral ischemia mechanism of P. elata.
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- 2019
5. Network pharmacology analysis and experimental validation of the antidepression mechanism of lactuside B.
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Niu, Bingxuan, Li, Shengying, Li, Chunyan, Song, Yu, Zhang, Hui, Niu, Huifang, Hua, Ruifang, Yan, Fulin, and Hai, Guangfan
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CELLULAR signal transduction ,PHARMACOLOGY ,MOLECULAR docking ,CARRIER proteins ,ASTROCYTES ,ANIMAL experimentation - Abstract
Background: Lactuside B (LB) is a novel active ingredient separated from Pterocypsela elata by our team. In our preliminary study, LB showed a therapeutic effect on depression. However, the underlying molecular mechanisms remain unclear. Objectives: This study was the first to study the pharmacological effects and mechanisms of LB on depression. Materials and Methods: Network pharmacology method was applied to screen the candidate targets and signaling pathways. Furthermore, molecular docking, cell test, and animal experiments were used to confirm the antidepression mechanisms. Results: The network pharmacology results showed 29 targets and 40 signaling pathways of LB on depression. Molecular docking showed that there was a strong binding effect between LB and each target (cAMP response element binding protein B [CREB], Ras, Raf, and ERK1/2). Cell tests indicated that the expressions of Ras and CREB in astrocyte cells (0.05–0.20 g/L of LB) exhibited significant differences (P < 0.05) compared with the model group, respectively. In animal tests, the expressions of Ras, ERK1/2, and Raf (12.5–50.0 μg/10 g of LB) showed a significant difference compared with mice in the model group (P < 0.05). Conclusion: These results indicated that the antidepression mechanism of LB was mainly associated with Ras signaling pathway, and the cAMP signaling pathway and PI3K-Akt signaling pathway may play an essential role. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Design, Synthesis and Biological Evaluation of Novel Thiazole-Fused Glaucocalyxin A Derivatives
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Zhang, Tao, primary, Li, Nianxian, primary, Zhou, Nanqian, primary, Ma, Wen, primary, Wei, Haiyuan, primary, Zhang, Bingxin, primary, Chen, Lianghui, primary, Hai, Guangfan, primary, Duan, Yingchao, primary, and Bai, Suping, primary
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- 2021
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7. Network pharmacology study on the active components of Pterocypsela elata and the mechanism of their effect against cerebral ischemia
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Niu,Bingxuan, Zhang,Hui, Li,Chunyan, Yan,Fulin, Song,Yu, Hai,Guangfan, Jiao,Yunjuan, and Feng,Yansheng
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Drug Design, Development and Therapy - Abstract
Bingxuan Niu,1 Hui Zhang,1 Chunyan Li,2 Fulin Yan,1,2 Yu Song,1 Guangfan Hai,1 Yunjuan Jiao,3 Yansheng Feng31College of Pharmacy, Xinxiang Medical University, Xingxiang, Henan Province 453003, People’s Republic of China; 2Sanquan College of Xinxiang Medical University, Xinxiang, Henan Province 453002, People’s Republic of China; 3Basic Medical College, Xinxiang Medical University, Xinxiang, Henan Province 453003, People’s Republic of ChinaCorrespondence: Fulin YanCollege of Pharmacy, Xinxiang Medical University, 601 Jinsui Avenue, Hongqi District, Xingxiang, Henan Province 453003, People’s Republic of ChinaTel +1 378 251 3326Email yannz2009@163.comObjective: The aim of this study was to identify the active anti-ischemic components of Pterocypsela elata (P. elata) using a network pharmacology approach to construct an effective component anti-cerebral ischemic target network and systematically analyze this medicinal material.Methods: Pharmacological studies have shown that P. elata has an obvious effect against cerebral ischemia. To identify the potential targets, 14 components of P. elata were docked to each structural element of the targets in the DRAR-CPI database by reverse docking technology. We then compared the identified potential targets with FDA-approved targets for stroke/cerebral infarction treatment in the DrugBank database and identified the active components of P. elata and their potential targets for stroke/cerebral infarction treatment. The active component-target networks were constructed using Cytoscape 3.5.1 software. The target protein-protein interactions were analyzed using the STRING database. KEGG pathway analysis and gene ontology (GO) enrichment analysis were performed through the Database for Annotation, Visualization and Integrated Discovery (DAVID).Results: There were 14 active components identified from P. elata and 21 potential targets identified for cerebral ischemia treatment, including carbonic anhydrase 2, ribosyldihydronicotinamide dehydrogenase, cholinesterase, and glutathione S-transferase P. The main involved pathways include metabolic pathways, complement and coagulation cascades and steroid hormone biosynthesis.Conclusion: Through a network pharmacology approach, we predicted the active components of P. elata and their potential targets for cerebral ischemia treatment. Our results provide new perspectives and clues for further studies on the anti-cerebral ischemia mechanism of P. elata.Keywords: network pharmacology, Pterocypsela elata, cerebral ischemia, molecular docking
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- 2019
8. Alterations in splenic function and gene expression in mice with depressive-like behavior induced by exposure to corticosterone
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Cui Taizhen, Xiaoman Jia, Heqin Zhan, Hai Guangfan, Yongji Shi, Feng Huang, Fu-Lin Yan, Zhenghang Zhao, Jixia Zhang, and Fan Yang
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0301 basic medicine ,differentially expressed genes ,Gene Expression ,Prefrontal Cortex ,Spleen ,Biology ,splenic function ,Hippocampus ,Pathogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Immune system ,Corticosterone ,Gene expression ,Genetics ,medicine ,Animals ,Cluster Analysis ,Regulation of gene expression ,Oncogene ,Behavior, Animal ,Depression ,Gene Expression Profiling ,General Medicine ,Articles ,depressive-like behavior ,Gene expression profiling ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Gene Expression Regulation ,protein levels ,Immunology ,histopathology ,spleen ,Female ,030217 neurology & neurosurgery ,Biomarkers - Abstract
Depressed patients present with increased cortisol levels and attenuated immune responses. However, little is known about the association between depression and the spleen, as this is the largest peripheral immune organ. In this study, we examined alterations in splenic function and gene expression in mice with depressive-like behavior, well as the expression of certain proteins in related pathways. A mouse model of depression was established with the use of corticosterone. Splenic function and histopathology were assessed using Wright and H&E staining. The Agilent Whole Mouse Genome Oligo Microarray containing >41,174 transcript probes was used to measure the levels of gene-expression in the spleens from control and model mice, and the levels of certain proteins associated with depression were measured by western blot analysis in the brain and spleen separately. We found that splenic function and immunity in the mice with depressive-like behavior were markedly impaired. A total of 53 genes exhibited a differential response in the mice with depressive-like behavior, 11 of which were more notable, including collagen, type VI, α5 (Col6a5), immunoglobulin superfamily, member 11 (Igsf11), D site albumin promoter binding protein (Dbp), tachykinin 2 (Tac2) and γ-aminobutyric acid B receptor 2 (Gabbr2). Pathway analysis revealed that the amino acid biosynthesis and the clock gene pathways were more meaningful among these genes. The levels of GABBR2, DBP and substance P (SP; encoded by the Tac2 gene) related proteins in the brain were markedly downregulated, and similar results were observed in the spleen. The anti-depressant, fluoxetine, reversed the changes in the levels of these proteins. The findings of our study regarding changes occurring in the spleen during depression may indirectly elucidate and shed light into the pathogenesis of depression and depressive-like behavior.
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- 2017
9. Design, Synthesis and Biological Evaluation of Novel Thiazole-Fused Glaucocalyxin A Derivatives
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Li Nianxian, Ma Wen, Nan-Qian Zhou, Tao Zhang, Haiyuan Wei, Hai Guangfan, Yingchao Duan, Bingxin Zhang, Chen Lianghui, and Su-Ping Bai
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chemistry.chemical_compound ,Design synthesis ,Chemistry ,Organic Chemistry ,Glaucocalyxin A ,Thiazole ,Combinatorial chemistry ,Biological evaluation - Published
- 2021
10. Alterations in splenic function and gene expression in mice with depressive-like behavior induced by exposure to corticosterone
- Author
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Zhan, Heqin, primary, Huang, Feng, additional, Yan, Fulin, additional, Zhao, Zhenghang, additional, Zhang, Jixia, additional, Cui, Taizhen, additional, Yang, Fan, additional, Hai, Guangfan, additional, Jia, Xiaoman, additional, and Shi, Yongji, additional
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- 2017
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11. Anti-hepatoma activity of a novel compound glaucocalyxin H in vivo and in vitro
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Bingxuan Niu, Cui Taizhen, Yu Song, Guo Lanqing, Han Jinfen, Huang Feng, Jia Yanlong, Bai Suping, Chong Zhang, and Hai Guangfan
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Male ,Carcinoma, Hepatocellular ,Pharmaceutical Science ,Down-Regulation ,Apoptosis ,Aquatic Science ,Glaucocalyxin H ,Mice ,Bcl-2-associated X protein ,Downregulation and upregulation ,In vivo ,Cell Line, Tumor ,Drug Discovery ,Animals ,Humans ,Medicine, Chinese Traditional ,Ecology, Evolution, Behavior and Systematics ,bcl-2-Associated X Protein ,Ecology ,biology ,Chemistry ,Liver Neoplasms ,General Medicine ,Hep G2 Cells ,Molecular biology ,Antineoplastic Agents, Phytogenic ,In vitro ,Up-Regulation ,Blot ,Proto-Oncogene Proteins c-bcl-2 ,Cell culture ,biology.protein ,Female ,Diterpenes, Kaurane ,Agronomy and Crop Science ,Drugs, Chinese Herbal ,Research Article - Abstract
Glaucocalyxin H (GLH) is a new compound isolated from a traditional Chinese medical herb Isodon japonica var. glaucocalyx which has been used for folk medicine. This study was carried out for the first time to investigate the potential role of GLH in anti-hepatoma activity and underlying mechanisms in it. GLH could inhibit the growth of tumor in mice and induce HepG2 cells to death as assessed by the tumor reduction assay, toxic assay, morphological change, and survival rate assay. Many antitumor drugs originated from plants could inhibit the growth of tumor by inducing cells to apoptosis. The morphological changes of HepG2 cells treated with different concentrations of GLH under fluorescence and electron microscope and apoptotic rates were detected to verify its effect on apoptosis. As shown in the study, GLH could induce HepG2 cells to apoptosis in a dose-dependent manner. Bcl2 and Bax proteins played important roles in apoptosis and the disequilibrium between Bcl2 and Bax might result in apoptosis. The expression of Bax protein was upregulated and Bcl2 protein was downregulated in HepG2 cells treated with GLH assessed by Western blotting, and they were in a dose-dependent manner. Taken together, GLH can inhibit the growth of hepatoma cells in vivo and in vitro by inducing cell apoptosis due to the decreased Bcl2 and increased Bax proteins suggesting that GLH could be a potential candidate as an anti-hepatoma agent for the therapeutic treatment of hepatoma.
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- 2014
12. Isolation, structural elucidation, and cytotoxicity of three new ent-kaurane diterpenoids from Isodon japonica var. glaucocalyx
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Hai Guangfan, Yun-Xiao Zhang, Dan-Dan Ye, Nan-Qian Zhou, Hui-Juan Liang, Su-Ping Bai, and Yong-Liang Yuan
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Magnetic Resonance Spectroscopy ,Stereochemistry ,Cell Survival ,In vitro cytotoxicity ,Pharmaceutical Science ,Crystallography, X-Ray ,Analytical Chemistry ,HeLa ,Glaucocalyxin H ,Inhibitory Concentration 50 ,Isodon japonica ,Cell Line, Tumor ,Drug Discovery ,Humans ,Cytotoxicity ,Ent kaurane ,Pharmacology ,Plants, Medicinal ,biology ,Molecular Structure ,Chemistry ,Organic Chemistry ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,Human tumor ,Plant Leaves ,Complementary and alternative medicine ,Cell culture ,Isodon ,Molecular Medicine ,Biological Assay ,Drug Screening Assays, Antitumor ,Diterpenes, Kaurane ,Drugs, Chinese Herbal - Abstract
Three new ENT-kaurane diterpenoids, glaucocalyxin H ( 1), glaucocalyxin I ( 2), and glaucocalyxin J ( 3), together with four known diterpenoids ( 4- 7), were isolated from the leaves of Isodon japonica Hara var. glaucocalyx. Their structures were elucidated by spectroscopic analysis, and the structures of compounds 2 and 3 were further confirmed by X-ray crystallographic analysis. Compounds 1, 4, and 5 were evaluated for their cytotoxicity IN VITRO against CE-1, U87, A-549, MCF-7, Hela, K-562, and HepG-2 human tumor cell lines. Compound 1 showed potent inhibitory activities against six tumor cell lines with IC (50) values ranging from 1.86-10.95 µM, and compounds 4 and 5 exhibited significant selective cytotoxicity on seven tumor cell lines.
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- 2012
13. Anti-Hepatoma Activity of a Novel Compound Glaucocalyxin H In Vivo and In Vitro
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Hai, Guangfan, primary, Zhang, Chong, additional, Jia, Yanlong, additional, Bai, Suping, additional, Han, Jinfen, additional, Guo, Lanqing, additional, Cui, Taizhen, additional, Niu, Bingxuan, additional, Huang, Feng, additional, and Song, Yu, additional
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- 2014
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14. [Effect of calcium supplement on superoxide dismutase and malonaldehyde of disuse osteoporosis in young rats].
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Tian Y, Li W, Hai G, and Zhang Y
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- Animals, Animals, Newborn, Bone Density, Calcium, Dietary pharmacology, Female, Malondialdehyde blood, Pregnancy, Random Allocation, Rats, Rats, Sprague-Dawley, Calcium pharmacology, Osteoporosis blood, Superoxide Dismutase blood
- Abstract
Effects of Ca supplement on the bone mineral density(BMD), red blood cell superoxide dismutase(SOD), plasma malonaldehyde(MDA) of disused osteoporosis were studied in this paper. Twenty five healthy pregnant SD rats were randomly divided into 5 groups, groups 2-5 were reproduced with patterns of animal osteoporosis. The diet in groups 1-2 was the basic diet, while the groups 3-5 experiment diets were supplemented with Ca. Selected 3 young rats, live with the mother rate each brood. The BMD, red blood cell SOD, plasma MDA were measured 37 d later. The results showed that BMD level of young rats in Ca supplement groups is higher than vigorous groups (P < 0.05). The MDA in vigorous group was higher than Ca supplement, groups(P < 0.01) and SOD was lower than high biologic calcium group(P < 0.05). It was suggested that Ca supplemented certain effect on red blood cell SOD, plasma MDA of disused osteoporosis.
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- 2003
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