112 results on '"Hai-Ming Xu"'
Search Results
2. Preliminary study on the role of aryl hydrocarbon receptor in the neurotoxicity of three typical bisphenol compounds (BPA, BPS and TBBPA) at environmentally relevant concentrations to adult zebrafish (Danio rerio)
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Jing Shan, Xiao-Fa Ma, Meng-Yu Wu, Yu-Jia Lin, Yi Wang, Rui Wang, Hong-Mei Li, Zhong-Lan Wu, and Hai-Ming Xu
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Bisphenol compounds ,Neurotoxicity ,Aryl hydrocarbon receptor ,Zebrafish ,Environmentally relevant concentrations ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Objective: This study was aimed to explore the role of AhR in the neurotoxicity of adult zebrafish induced by three typical bisphenol compounds (BPA, BPS, TBBPA) at environmentally relevant doses. Methods: The adult zebrafish were randomly divided into solvent control group (DMSO) and AhR inhibitor CH223191 (CH) group (0.05 μmol/L), bisphenol exposure groups (10, 100, 1000 nmol/L) and combined exposure groups (0.05 μmol/L CH and 1000 nmol/L bisphenol compounds). Each tank contained 8 fish (4 male and 4 female), and two parallel tanks were set synchronously. After 30 days of exposure, zebrafish were put on ice plate for anesthesia, weighed and measured for body length, and dissected for brain tissue. The gene expression was detected by RT-qPCR, and the activities of antioxidant enzymes were detected by commercial kits. SPSS 26.0 was used to analyze the data. Additionally, GO, KEGG and principal component analysis (PCA) were carried out. Results: Compared with the solvent control group, there were no significant differences in body weight and length among the exposed groups. In general, exposure to bisphenol compounds could affect the expression of Ahr2 and AhR target genes (cyp1a1, cyp1a2, and cyp1c1), key genes of neural function (elavl3, gfap, mbp, syn2a, gap43, Zn5, shha, and ache), oxidative stress related genes (nrf2, gpx1a, gstp1/gstp1.2, gstp2/gstp1.1, sod1, sod2, and cat), and the activities of antioxidant enzymes (SOD, CAT and GSH-Px/GPX) in zebrafish brain tissue to some extent. Compared with the groups exposed to bisphenols alone, CH could antagonize the above interference effects caused by bisphenols to some extent. Therefore, the toxic effects of BPA, BPS and TBBPA might be produced through similar mechanisms. Conclusion: Environmentally related doses of bisphenols (BPA, BPS, TBBPA) could disturb the expression of key molecules of oxidative stress and neural function through activating the AhR signaling pathway, and ultimately lead to neurotoxicity.
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- 2023
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3. Unique regulatory roles of ncRNAs changed by PM2.5 in human diseases
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Bo He, Hai-Ming Xu, Hao-Wen Liu, and Yin-Feng Zhang
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Ambient particulate matter ,LncRNA ,MiRNA ,CircRNA ,Biomarker ,Diagnosis ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
PM2.5 is a type of particulate matter with an aerodynamic diameter smaller than 2.5 µm, and exposure to PM2.5 can adversely damage human health. PM2.5 may impair health through oxidative stress, inflammatory reactions, immune function alterations and chromosome or DNA damage. Through increasing in-depth studies, researchers have found that noncoding RNAs (ncRNAs), particularly microRNAs (miRNAs), circular RNAs (circRNAs) as well as long noncoding RNAs (lncRNAs), might play significant roles in PM2.5-related human diseases via some of the abovementioned mechanisms. Therefore, in this review, we mainly discuss the regulatory function of ncRNAs altered by PM2.5 in human diseases and summarize the potential molecular mechanisms. The findings reveal that these ncRNAs might induce or promote diseases via inflammation, the oxidative stress response, cell autophagy, apoptosis, cell junction damage, altered cell proliferation, malignant cell transformation, disruption of synaptic function and abnormalities in the differentiation and status of immune cells. Moreover, according to a bioinformatics analysis, the altered expression of potential genes caused by these ncRNAs might be related to the development of some human diseases. Furthermore, some ncRNAs, including lncRNAs, miRNAs and circRNAs, or processes in which they are involved may be used as biomarkers for relevant diseases and potential targets to prevent these diseases. Additionally, we performed a meta-analysis to identify more promising diagnostic ncRNAs as biomarkers for related diseases.
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- 2023
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4. Preliminary Study on the Effect and Molecular Mechanism of Tetrandrine in Alleviating Pulmonary Inflammation and Fibrosis Induced by Silicon Dioxide
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Yi Wang, Bin Cheng, Yu-Jia Lin, Rui Wang, Jie Xuan, and Hai-Ming Xu
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silicosis ,tetrandrine ,SiO2 ,lung inflammation ,pulmonary fibrosis ,autophagy ,Chemical technology ,TP1-1185 - Abstract
This study aims to explore the molecular mechanism of tetrandrine (Tet) in alleviating pulmonary inflammation and fibrosis induced by silica (SiO2) from the perspective of autophagy. C57BL/6J mice were selected as experimental animals, and SiO2 was exposed by intranasal instillation. Tet was intervened by oral gavage. The mice were euthanized on the 7th and 42nd day of SiO2 exposure, and lung tissues were collected for histopathological, molecular biological, immunological, and transmission electron microscopy analysis. The results showed that SiO2 exposure could lead to significant lung inflammation and fibrosis, while Tet could significantly reduce SiO2 exposure-induced lung inflammation and fibrosis. Molecular mechanism research indicated that, compared with SiO2 expose group, Tet intervention could significantly reduce the expression levels of inflammatory cytokines and fibrosis markers (TNF-α, IL-1β, MCP-1, TGF-β1, HYP, Col-I, and Fn), and regulate the expression of key molecules ATG7, microtubule-associated protein 1 light chain 3B (LC3B), and P62 in the autophagy pathway to improve the blocking of autophagic flux, promote the recovery of autophagic lysosomal system function, and inhibit apoptosis. In summary, Tet can alleviate silica-induced lung inflammation and fibrosis, which may be achieved by regulating the expression of key molecules in the autophagy process and associated apoptotic pathway.
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- 2023
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5. Genetic architecture of protein expression and its regulation in the mouse brain
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Alyssa Erickson, Suiping Zhou, Jie Luo, Ling Li, Xin Huang, Zachary Even, He Huang, Hai-Ming Xu, Junmin Peng, Lu Lu, and Xusheng Wang
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Proteome ,Mouse ,Brain ,Protein expression ,Allele-specific expression ,Protein regulation ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Natural variation in protein expression is common in all organisms and contributes to phenotypic differences among individuals. While variation in gene expression at the transcript level has been extensively investigated, the genetic mechanisms underlying variation in protein expression have lagged considerably behind. Here we investigate genetic architecture of protein expression by profiling a deep mouse brain proteome of two inbred strains, C57BL/6 J (B6) and DBA/2 J (D2), and their reciprocal F1 hybrids using two-dimensional liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) technology. Results By comparing protein expression levels in the four mouse strains, we observed 329 statistically significant differentially expressed proteins between the two parental strains and characterized the genetic basis of protein expression. We further applied a proteogenomic approach to detect variant peptides and define protein allele-specific expression (pASE), identifying 33 variant peptides with cis-effects and 17 variant peptides showing trans-effects. Comparison of regulation at transcript and protein levels show a significant divergence. Conclusions The results provide a comprehensive analysis of genetic architecture of protein expression and the contribution of cis- and trans-acting regulatory differences to protein expression.
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- 2021
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6. Role of non-neuronal cholinergic system in the early stage response of epithelial-mesenchymal transformation related markers in A549 cells induced by coal particles
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Meng-Yu Wu, Xin-Chen Shi, Jing Shan, Rui Wang, Yi Wang, Jie Li, Da-Nian Tian, and Hai-Ming Xu
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Non-neuronal cholinergic system ,Epithelial-mesenchymal transformation ,Coal particles ,Oxidative stress ,Inflammatory response ,A549 cells ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Objective: This study was aimed to investigate the role of non-neuronal cholinergic system (NNCS) in the early stage response of epithelial-mesenchymal transformation (EMT) related markers in human lung adenocarcinoma A549 cells induced by coal particles. Methods: A549 cells were exposed to different concentrations of GBW11110K, GBW11126D and exogenous acetylcholinesterase (AChE) (the exposure doses were determined according to the results of CCK-8 experiment, and the doses that had no significant effects on cell viability were selected) for 24 h. After exposure, the indexes of oxidative stress (SOD and MDA), inflammatory factors (IL-6 and TNF-α), EMT marker proteins (E-cadherin and vimentin), AChE enzymatic activity and mRNA expression levels of different types of acetylcholine receptors (CHRM3, CHRM5, CHRNA5, CHRNA7, CHRNA9 and CHRNB2) were determined. Results: GBW11110K and GBW11126D exposure could lead to the following injury effects: the levels of oxidative stress and inflammatory factors changed to a certain extent (SOD decreased gradually, while MDA, IL-6 and TNF-α increased). The protein level of E-cadherin decreased while the vimentin level increased (P < 0.05), suggesting the occurrence of EMT. The AChE enzymatic activity decreased gradually. The expression of acetylcholine receptor mRNA changed as follows (GBW11110K/GBW11126D: CHRM3 (↑↑), CHRM5 (↓↓), CHRNA5 (↓↓), CHRNA7 (↓↓), CHRNA9 (– ↑), CHRNB2 (– –). The addition of exogenous AChE recombinant protein could antagonize the damage effects caused by the coal particles to a certain extent. Conclusion: The coal particle exposure could induce the change of oxidative stress response, inflammatory response and EMT related markers, down-regulate the AChE enzymatic activity, and interfere the mRNA expression levels of AChRs in A549 cells. The addition of exogenous AChE recombinant protein could reverse the above effects to a certain extent.
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- 2022
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7. Exosomal noncoding RNAs in central nervous system diseases: biological functions and potential clinical applications
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Zhong-Yu Wang, Zeng-Jin Wen, Hai-Ming Xu, Yu Zhang, and Yin-Feng Zhang
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exosome ,noncoding RNA ,central nervous system ,biomarkers ,pathophysiology ,therapy ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Central nervous system (CNS) disease is a general term for a series of complex and diverse diseases, including Alzheimer’s disease (AD), Parkinson’s disease (PD), multiple sclerosis (MS), CNS tumors, stroke, epilepsy, and amyotrophic lateral sclerosis (ALS). Interneuron and neuron-glia cells communicate with each other through their homeostatic microenvironment. Exosomes in the microenvironment have crucial impacts on interneuron and neuron-glia cells by transferring their contents, such as proteins, lipids, and ncRNAs, constituting a novel form of cell-to-cell interaction and communication. Exosomal noncoding RNAs (ncRNAs), including microRNAs (miRNAs), long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), and PIWI-interacting RNAs (piRNAs), regulate physiological functions and maintain CNS homeostasis. Exosomes are regarded as extracellular messengers that transfer ncRNAs between neurons and body fluids due to their ability to cross the blood-brain barrier. This review aims to summarize the current understanding of exosomal ncRNAs in CNS diseases, including prospective diagnostic biomarkers, pathological regulators, therapeutic strategies and clinical applications. We also provide an all-sided discussion of the comparison with some similar CNS diseases and the main limitations and challenges for exosomal ncRNAs in clinical applications.
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- 2022
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8. Study on the molecular mechanisms of tetrandrine against pulmonary fibrosis based on network pharmacology, molecular docking and experimental verification
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Jie Li, Yi Wang, Rui Wang, Meng-Yu Wu, Jing Shan, Ying-Chi Zhang, and Hai-Ming Xu
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Pulmonary fibrosis ,Tetrandrine ,Network pharmacology ,Target prediction ,Molecular docking ,Experimental verification ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Aims: This study aims to screen the potential targets of tetrandrine (Tet) against pulmonary fibrosis (PF) based on network pharmacological analysis, molecular docking and experimental verification. Main methods: The network pharmacology methods were employed to predict targets, construct Tet-PF-intersection target-pathway networks, and screen the candidate targets. The molecular docking was performed using AutoDockTools1.5.6. TGF-β1-induced human lung adenocarcinoma A549 cells were used as an in vitro experimental verification model, taking dexamethasone (Dex) as the positive control, to verify the effects of Tet on the mRNA expression of the candidate targets. Key findings: Six candidate targets were predicted based on network pharmacology and molecular docking, namely PIK3CA, PDPK1, RAC1, PTK2, KDR, and RPS6KB1. The experimental verification results showed that Dex and Tet presented quite different pharmacological effects. Specifically, compared with the model group, both Dex and Tet (5 μΜ) significantly increased the mRNA expression of PIK3CA and KDR (P < 0.001). Dex up-regulated the mRNA expression of PDPK1 and RAC1, while Tet (1.25 μΜ) down-regulated (P < 0.001). Dex up-regulated the mRNA expression of PTK2, but Tet had no effect. Dex down-regulated RPS6KB1 mRNA expression, while Tet (5 μΜ) up-regulated (P < 0.01). Significance: Combined with the results of theoretical calculation and experimental verification, and considering the roles of these targets in the pathogenesis of PF, Tet might antagonize PF by acting on PDPK1 and RAC1. The results of this study will provide scientific reference for the prevention and clinical diagnosis and treatment of PF.
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- 2022
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9. Bisphenol B disrupts testis differentiation partly via the estrogen receptor-mediated pathway and subsequently causes testicular dysgenesis in Xenopus laevis
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Hong-Mei Li, Yuan-Yuan Li, Ying-Chi Zhang, Jin-Bo Li, Hai-Ming Xu, Yi-Ming Xiong, and Zhan-Fen Qin
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Bisphenol B ,Xenopus laevis ,Testis differentiation ,Testicular dysgenesis ,Estrogenic activity ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
There is growing concern about adverse effects of bisphenol A alternatives including bisphenol B (BPB) due to their estrogenic activity. However, limited data are available concerning the influences of BPB on male reproductive development in vertebrates, especially in amphibians, which are believed to be susceptible to estrogenic chemicals. The present study investigated the effects of 10, 100 and 1000 nM BPB (2.42, 24.2 and 242 μg/L) on testis development in Xenopus laevis, a model amphibian species for studying gonadal feminization. We found that exposure to BPB from stages 45/46 to 52 resulted in down-regulation of testis-biased gene expression and up-regulation of ovary-biased gene and vitellogenin (vtgb1) expression in gonad-mesonephros complexes (GMCs) of tadpoles at stage 52, coupled with suppressed cell proliferation in testes and reduced gonadal metameres, resembling the effects of 17ß-estradiol. Moreover, an estrogen receptor (ER) antagonist ICI 182780 antagonized BPB-caused up-regulation of ovary-biased gene and vtgb1 expression to some degree, indicating that the effects of BPB on X. laevis testis differentiation could be partly mediated by ER. All observations demonstrate that early exposure to BPB inhibited testis differentiation and exerted certain feminizing effects during gonadal differentiation. When exposure was extended to post-metamorphosis, testes exhibited histological and morphological abnormalities including segmented, discontinuous and fragmented shapes, besides altered sex-dimorphic gene expression. Notably, most of BPB-caused alterations were not concentration-dependent, but the lowest concentration indeed exerted significant effects. Overall, our study for the first time reveals that low concentrations of BPB can disrupt testis differentiation partly due to its estrogenic activity and subsequently cause testicular dysgenesis after metamorphosis, highlighting its reproductive risk to amphibians and other vertebrates including humans. Our finding also implies that estrogenic chemicals-caused testis differentiation inhibition at tadpole stages could predict later testicular dysgenesis after metamorphosis, meaning a possibility of early detection of abnormal testis development caused by estrogenic chemicals.
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- 2022
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10. Preliminary Study on the Protective Effects and Molecular Mechanism of Procyanidins against PFOS-Induced Glucose-Stimulated Insulin Secretion Impairment in INS-1 Cells
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Hai-Ming Xu, Meng-Yu Wu, Xin-Chen Shi, Ke-Liang Liu, Ying-Chi Zhang, Yin-Feng Zhang, and Hong-Mei Li
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PFOS ,glucose-stimulated insulin secretion (GSIS) ,insulin level ,procyanidins ,reactive oxygen species ,INS-1 cells ,Chemical technology ,TP1-1185 - Abstract
This study aimed to investigate the effects of perfluorooctanesulfonic acid (PFOS) exposure on glucose-stimulated insulin secretion (GSIS) of rat insulinoma (INS-1) cells and the potential protective effects of procyanidins (PC). The effects of PFOS and/or PC on GSIS of INS-1 cells were investigated after 48 h of exposure (protein level: insulin; gene level: glucose transporter 2 (Glut2), glucokinase (Gck), and insulin). Subsequently, the effects of exposure on the intracellular reactive oxygen species (ROS) activity were measured. Compared to the control group, PFOS exposure (12.5, 25, and 50 μM) for 48 h had no significant effect on the viability of INS-1 cells. PFOS exposure (50 μM) could reduce the level of insulin secretion and reduce the relative mRNA expression levels of Glut2, Gck, and insulin. It is worth noting that PC could partially reverse the damaging effect caused by PFOS. Significantly, there was an increase in ROS after exposure to PFOS and a decline after PC intervention. PFOS could affect the normal physiological function of GSIS in INS-1 cells. PC, a plant natural product, could effectively alleviate the damage caused by PFOS by inhibiting ROS activity.
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- 2023
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11. Subsampling Technique to Estimate Variance Component for UK-Biobank Traits
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Ting Xu, Guo-An Qi, Jun Zhu, Hai-Ming Xu, and Guo-Bo Chen
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polygenicity ,UK Biobank ,subsampling estimator ,effective number of markers ,Haseman-Elston regression ,Genetics ,QH426-470 - Abstract
The estimation of heritability has been an important question in statistical genetics. Due to the clear mathematical properties, the modified Haseman–Elston regression has been found a bridge that connects and develops various parallel heritability estimation methods. With the increasing sample size, estimating heritability for biobank-scale data poses a challenge for statistical computation, in particular that the calculation of the genetic relationship matrix is a huge challenge in statistical computation. Using the Haseman–Elston framework, in this study we explicitly analyzed the mathematical structure of the key term tr(KTK), the trace of high-order term of the genetic relationship matrix, a component involved in the estimation procedure. In this study, we proposed two estimators, which can estimate tr(KTK) with greatly reduced sampling variance compared to the existing method under the same computational complexity. We applied this method to 81 traits in UK Biobank data and compared the chromosome-wise partition heritability with the whole-genome heritability, also as an approach for testing polygenicity.
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- 2021
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12. Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139
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Li-Cheng Gong, Hai-Ming Xu, Gong-Liang Guo, Tao Zhang, Jing-Wei Shi, and Chang Chang
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Long non-coding RNA H19 ,Acute myocardial infarction ,MiR-139 ,Sox8 ,PI3K/AKT ,MTOR ,MAPK ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Acute myocardial infarction (AMI) occurs when blood supply to the heart is diminished (ischemia) for long time; ischemia is primarily caused due to hypoxia. The present study evaluated the effects of long non-coding RNA H19 on hypoxic rat H9c2 cells and mouse HL-1 cells. Methods: Hypoxic injury was confirmed by measuring cell viability, migration and invasion, and apoptosis using MTT, Transwell and flow cytometry assays, respectively. H19 expression after hypoxia was estimated by qRT-PCR. We then measured the effects of non-physiologically expressed H19, knockdown of miR-139 with or without H19 silence, and abnormally expressed Sox8 on hypoxia-induced H9c2 cells. Moreover, the interacted miRNA for H19 and downstream target gene were virtually screened and verified. The involved signaling pathways and the effects of abnormally expressed H19 on contractility of HL-1 cells were explored via Western blot analysis. Results: Hypoxia induced decreases of cell viability, migration and invasion, increase of cell apoptosis and up-regulation of H19. Knockdown of H19 increased hypoxia-induced injury in H9c2 cells. H19 acted as a sponge for miR-139 and H19 knockdown aggravated hypoxia-induced injury by up-regulating miR-139. Sox8 was identified as a target of miR-139, and its expression was negatively regulated by miR-139. The mechanistic studies revealed that overexpression of Sox8 might decrease hypoxia-induced cell injury by activating the PI3K/AKT/mTOR pathway and MAPK. Besides, H19 promoted contractility of HL-1 cells. Conclusion: These findings suggest that H19 alleviates hypoxia-induced myocardial cell injury by miR-139-mediated up-regulation of Sox8, along with activation of the PI3K/AKT/mTOR pathway and MAPK.
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- 2017
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13. Role of short-wavelength filtering lenses in delaying myopia progression and amelioration of asthenopia in juveniles
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Hai-Lan Zhao, Jin Jiang, Jie Yu, and Hai-Ming Xu
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1267 ,short-wavelength filtering lenses ,asthenopia ,juvenile myopia ,Ophthalmology ,RE1-994 - Abstract
AIM: To evaluate the positive effects of blue-violet light filtering lenses in delaying myopia and relieving asthenopia in juveniles. METHODS: Sixty ametropia juveniles (aged range, 11-15y) were randomized into two groups: the test group (30 children, 60 eyes), wearing blue-violet light filtering lenses; and the control group (30 children, 60 eyes), wearing ordinary aspherical lenses. Baseline refractive power of the affected eyes and axial length of the two groups was recorded. After 1-year, the patients underwent contrast sensitivity (glare and non-glare under bright and dark conditions), accommodation-related testing, asthenopia questionnaire assessment, and adverse reaction questionnaire assessment. RESULTS: After 1y of wearing the filtering lenses, changes in refractive power and axial length were not significantly different between the two groups (P>0.05). Under bright conditions, the contrast sensitivities at low and medium-frequency grating (vision angles of 6.3°, 4.0°, and 2.5°) with glare in the test group were significantly higher than in the control group (P0.05). In the test group, the amplitude of accommodation, accommodative lag, and accommodative sensitivity of patients wearing glasses for 6 and 12mo were significantly elevated (P0.05), and the asthenopia grating was not significantly decreased (P>0.05). In addition, after wearing glasses for 6 to 12mo, the asthenopia grating of patients in the test group decreased significantly compared with the control group (P0.05). CONCLUSION: A 1-year follow-up reveal that compare with ordinary glasses, short-wavelength filtering lenses (blue/violet-light filters) increase the low- and medium-frequency contrast sensitivity under bright conditions and improved accommodation. They effectively relieved asthenopia without severe adverse reactions, suggesting potential for clinical application. However, no significant advantages in terms of refractive power or axial length progression were found compared with ordinary aspheric lenses.
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- 2017
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14. Crosstalk between MicroRNAs and Peroxisome Proliferator-Activated Receptors and Their Emerging Regulatory Roles in Cardiovascular Pathophysiology
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Yin-Feng Zhang, Hai-Ming Xu, Fei Yu, Man Wang, Meng-Yang Li, Tao Xu, Yan-Yan Gao, Jian-Xun Wang, and Pei-Feng Li
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Biology (General) ,QH301-705.5 - Abstract
Peroxisome proliferator-activated receptors (PPARs) play vital roles in cardiovascular pathophysiology, such as energy balance, cell proliferation/apoptosis, inflammatory response, and adipocyte differentiation. These vital roles make PPARs potential targets for therapeutic prevention of cardiovascular diseases (CVDs). Emerging evidence indicates that the crosstalk of microRNAs (miRNAs) and PPARs contributes greatly to CVD pathogenesis. PPARs are inhibited by miRNAs at posttranscriptional mechanisms in the progress of pulmonary hypertension and vascular dysfunction involving cell proliferation/apoptosis, communication, and normal function of endothelial cells and vascular smooth muscle cells. In the development of atherosclerosis and stroke, the activation of PPARs could change the transcripts of target miRNA through miRNA signalling. Furthermore, the mutual regulation of PPARs and miRNAs involves cell proliferation/apoptosis, cardiac remodeling, and dysfunction in heart diseases. In addition, obesity, an important cardiovascular risk, is modulated by the regulatory axis of PPARs/miRNAs, including adipogenesis, adipocyte dysfunction, insulin resistance, and macrophage polarization in adipose tissue. In this review, the crosstalk of PPARs and miRNAs and their emerging regulatory roles are summarized in the context of CVDs and risks. This provides an understanding of the underlying mechanism of the biological process related to CVD pathophysiology involving the interaction of PPARs and miRNAs and will lead to the development of PPARs/miRNAs as effective anti-CVD medications.
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- 2018
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15. Multivariate dimensionality reduction approaches to identify gene-gene and gene-environment interactions underlying multiple complex traits.
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Hai-Ming Xu, Xi-Wei Sun, Ting Qi, Wan-Yu Lin, Nianjun Liu, and Xiang-Yang Lou
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Medicine ,Science - Abstract
The elusive but ubiquitous multifactor interactions represent a stumbling block that urgently needs to be removed in searching for determinants involved in human complex diseases. The dimensionality reduction approaches are a promising tool for this task. Many complex diseases exhibit composite syndromes required to be measured in a cluster of clinical traits with varying correlations and/or are inherently longitudinal in nature (changing over time and measured dynamically at multiple time points). A multivariate approach for detecting interactions is thus greatly needed on the purposes of handling a multifaceted phenotype and longitudinal data, as well as improving statistical power for multiple significance testing via a two-stage testing procedure that involves a multivariate analysis for grouped phenotypes followed by univariate analysis for the phenotypes in the significant group(s). In this article, we propose a multivariate extension of generalized multifactor dimensionality reduction (GMDR) based on multivariate generalized linear, multivariate quasi-likelihood and generalized estimating equations models. Simulations and real data analysis for the cohort from the Study of Addiction: Genetics and Environment are performed to investigate the properties and performance of the proposed method, as compared with the univariate method. The results suggest that the proposed multivariate GMDR substantially boosts statistical power.
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- 2014
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16. Practical and theoretical considerations in study design for detecting gene-gene interactions using MDR and GMDR approaches.
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Guo-Bo Chen, Yi Xu, Hai-Ming Xu, Ming D Li, Jun Zhu, and Xiang-Yang Lou
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Medicine ,Science - Abstract
Detection of interacting risk factors for complex traits is challenging. The choice of an appropriate method, sample size, and allocation of cases and controls are serious concerns. To provide empirical guidelines for planning such studies and data analyses, we investigated the performance of the multifactor dimensionality reduction (MDR) and generalized MDR (GMDR) methods under various experimental scenarios. We developed the mathematical expectation of accuracy and used it as an indicator parameter to perform a gene-gene interaction study. We then examined the statistical power of GMDR and MDR within the plausible range of accuracy (0.50∼0.65) reported in the literature. The GMDR with covariate adjustment had a power of >80% in a case-control design with a sample size of ≥2000, with theoretical accuracy ranging from 0.56 to 0.62. However, when the accuracy was
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- 2011
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17. ProtoTransfer: Cross-Modal Prototype Transfer for Point Cloud Segmentation.
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Pin Tang, Hai-Ming Xu, and Chao Ma 0004
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- 2023
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18. DETRDistill: A Universal Knowledge Distillation Framework for DETR-families.
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Jiahao Chang, Shuo Wang, Hai-Ming Xu, Zehui Chen, Chenhongyi Yang, and Feng Zhao 0004
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- 2023
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19. Progressive Feature Adjustment for Semi-supervised Learning from Pretrained Models.
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Hai-Ming Xu, Lingqiao Liu, Hao Chen, Ehsan Abbasnejad, and Rafael Felix
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- 2023
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20. Towards Domain Generalization for Multi-view 3D Object Detection in Bird-Eye-View.
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Shuo Wang, Xinhai Zhao, Hai-Ming Xu, Zehui Chen, Dameng Yu, Jiahao Chang, Zhen Yang, and Feng Zhao 0004
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- 2023
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21. Progressive Class Semantic Matching for Semi-supervised Text Classification.
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Hai-Ming Xu, Lingqiao Liu, and Ehsan Abbasnejad
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- 2022
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22. Semi-supervised Learning via Conditional Rotation Angle Estimation.
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Hai-Ming Xu, Lingqiao Liu, and Dong Gong
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- 2021
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23. Two-stage Decision Improves Open-Set Panoptic Segmentation.
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Hai-Ming Xu, Hao Chen, Lingqiao Liu, and Yufei Yin
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- 2022
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24. Improving Fine-Grained Visual Recognition in Low Data Regimes via Self-Boosting Attention Mechanism.
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Yangyang Shu, Baosheng Yu, Hai-Ming Xu, and Lingqiao Liu
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- 2022
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25. Semi-supervised Semantic Segmentation with Prototype-based Consistency Regularization.
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Hai-Ming Xu, Lingqiao Liu, Qiuchen Bian, and Zhen Yang
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- 2022
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26. Semi-supervised Learning via Conditional Rotation Angle Estimation.
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Hai-Ming Xu, Lingqiao Liu, and Dong Gong
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- 2020
27. Low concentrations of benzophenone-type UV-filters impair testis development in the amphibian Xenopus laevis
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Hong-Mei Li, Ying-Chi Zhang, Yuan-Yuan Li, Qing-Qing Zhu, Jie Li, Hai-Ming Xu, Yi-Ming Xiong, and Zhan-Fen Qin
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Health, Toxicology and Mutagenesis ,Aquatic Science - Abstract
Benzophenone-type UV filters (BPs) are ubiquitous contaminants in aquatic environments, possibly posing ecological risks to aquatic populations. So far, little is known about the potential adverse effects of BPs on amphibians. Given their potential estrogenic property, we investigated the detrimental effects of the commonly used BPs, BP-3, BP-2, and BP-1, on testis development in amphibians using Xenopus laevis as a model species. Following exposure to 10, 100, 1000 nM BP-3, BP-2, or BP-1 from stages 45/46 to 52, tadpoles presented morphological abnormal testes, characterized by reduced gonomere size and testis area, coupled with suppressed cell proliferation. Meanwhile, the downregulation of testis-biased gene expression and the upregulation of ovary-biased gene expression were observed in BPs-treated testes. Moreover, the estrogen receptor (ER) antagonist ICI 182780 significantly antagonized ovary-biased gene upregulation caused by BPs, suggesting that the effects of BPs on testis differentiation could be mediated by ER, at least partially. Of note, the effects of BPs were not concentration-dependent, but the lowest concentration generally exerted significant effects. Altogether, these observations indicate that the three BPs inhibited testis differentiation and exerted feminizing effects. Importantly, when BP-2 exposure was extended to two months post-metamorphosis, testes of froglets were generally less-developed, with relatively fewer spermatocytes, more spermatogonia, and poorly formed seminiferous tubules. Considering the fact that the lowest concentration (10 nM) of BPs in this study are detectable in aquatic environments, we conclude that BP-3, BP-2, and BP-1, even at environmentally relevant concentrations, can retard testis differentiation at pre-metamorphic stages and cause testis dysgenesis after metamorphosis in the amphibian X. laevis. Our findings suggest that ubiquitous BPs in aquatic environments could pose a potential risk to amphibians.
- Published
- 2022
28. Searching across-cohort relatives via encrypted genotype regression
- Author
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Qixin Zhang, Tianzi Liu, Xinxin Guo, Jianxin Zhen, Kan Bao, Meng-yuan Yang, Saber Khederzadeh, Fang Zhou, Xiaotong Han, Qiwen Zheng, Peilin Jia, Xiaohu Ding, Mingguang He, Xin Zou, Hongxin Zhang, Ji He, Xiaofeng Zhu, Yangyun Zou, Sijia Lu, Daru Lu, Hongyan Chen, Changqing Zeng, Fan Liu, Hou-Feng Zheng, Siyang Liu, Hai-Ming Xu, and Guo-Bo Chen
- Abstract
Identifying relatives across cohorts makes one of the basic routines for genomic data. As conventional such practice often requires explicit genomic data sharing, it is easily hampered by privacy or ethical constraints. In this study, using our proposed scheme for genomic encryption we developedencG-reg, a regression approach that is able to detect relatives of various degrees based on encrypted genomic data. The encryption properties ofencG-regis built on random matrix theory, which masks the original genotypic matrix but still provides controllable precision to that of direct individual-level genotype data. After having found tractable eighth-order moments for encrypted genotype, we established connection between the dimension of a random matrix and the required precision of a study.encG-regconsequently led to balanced i) false positive and false negative rates and ii) the computational cost and the degree of relatives to be searched. We validatedencG-regin 485,158 UKBiobank multi-ethnical samples, and the resolution ofencG-regwas comparable with the conventional method such as KING. In a more complex application, we launched a fine-devised multi-center collaboration across 6 research institutes in China, covering 11 cohorts of 64,091 GWAS samples. In both examples,encG-regrobustly identified and validated relatives existing across the cohorts even under various ethnical background and different genotypic qualities.
- Published
- 2022
29. Genetic dissection of the bacterial blight disease resistance in super hybrid rice RILs using genome-wide association study
- Author
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Hai-Han ZHAO, Wang-Min LIAN, Xiao-Deng ZHAN, Hai-Ming XU, Ying-Xin ZHANG, Shi-Hua CHENG, Xiang-Yang LOU, Li-Yong CAO, and Yong-Bo HONG
- Subjects
Plant Science ,Agronomy and Crop Science ,Biotechnology - Published
- 2021
30. Multi-omics analysis reveals expression complexity and functional diversity of mouse kinome
- Author
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Xin Huang, Ling Li, Suiping Zhou, Dehui Kong, Jie Luo, Lu Lu, Hai‐Ming Xu, and Xusheng Wang
- Subjects
Mice, Inbred C57BL ,Mice ,Phenotype ,Species Specificity ,Mice, Inbred DBA ,Animals ,Molecular Biology ,Biochemistry ,Protein Kinases - Abstract
Protein kinases are a crucial component of signaling pathways involved in a wide range of cellular responses, including growth, proliferation, differentiation, and migration. Systematic investigation of protein kinases is critical to better understand phosphorylation-mediated signaling pathways and may provide insights into the development of potential therapeutic drug targets. Here we perform a systems-level analysis of the mouse kinome by analyzing multi-omics data. We used bulk and single-cell transcriptomic data from the C57BL/6J mouse strain to define tissue- and cell-type-specific expression of protein kinases, followed by investigating variations in sequence and expression between C57BL/6J and DBA/2J strains. We then profiled a deep brain phosphoproteome from C57BL/6J and DBA/2J strains as well as their reciprocal hybrids to infer the activity of the mouse kinome. Finally, we performed phenome-wide association analysis using the BXD recombinant inbred (RI) mice (a cross between C57BL/6J and DBA/2J strains) to identify any associations between variants in protein kinases and phenotypes. Collectively, our study provides a comprehensive analysis of the mouse kinome by investigating genetic sequence variation, tissue-specific expression patterns, and associations with downstream phenotypes.
- Published
- 2022
31. Hsa-miR-379 down-regulates Rac1/MLK3/JNK/AP-1/Collagen I cascade reaction by targeting connective tissue growth factor in human alveolar basal epithelial A549 cells
- Author
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Jing Shan, Meng-Yu Wu, Ying-Chi Zhang, Yu-Jia Lin, Bin Cheng, Yan-Rong Gao, Zhi-Hong Liu, and Hai-Ming Xu
- Subjects
Immunology ,Immunology and Allergy ,Hematology ,Molecular Biology ,Biochemistry - Published
- 2023
32. A primal perspective for indefinite kernel SVM problem
- Author
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Hai-Ming Xu, Yunyun Wang, Xiaohong Chen, and Hui Xue
- Subjects
Line search ,General Computer Science ,Computer science ,020207 software engineering ,02 engineering and technology ,Function (mathematics) ,Theoretical Computer Science ,Support vector machine ,Kernel (linear algebra) ,Rate of convergence ,0202 electrical engineering, electronic engineering, information engineering ,020201 artificial intelligence & image processing ,Descent direction ,Convex function ,Focus (optics) ,Algorithm - Abstract
Indefinite kernel support vector machine (IKSVM) has recently attracted increasing attentions in machine learning. Since IKSVM essentially is a non-convex problem, existing algorithms either change the spectrum of indefinite kernel directly but risking losing some valuable information or solve the dual form of IKSVM whereas suffering from a dual gap problem. In this paper, we propose a primal perspective for solving the problem. That is, we directly focus on the primal form of IKSVM and present a novel algorithm termed as IKSVM-DC for binary and multi-class classification. Concretely, according to the characteristics of the spectrum for the indefinite kernel matrix, IKSVM-DC decomposes the primal function into the subtraction of two convex functions as a difference of convex functions (DC) programming. To accelerate convergence rate, IKSVM-DC combines the classical DC algorithm with a line search step along the descent direction at each iteration. Furthermore, we construct a multi-class IKSVM model which can classify multiple classes in a unified form. A theoretical analysis is then presented to validate that IKSVM-DC can converge to a local minimum. Finally, we conduct experiments on both binary and multi-class datasets and the experimental results show that IKSVM-DC is superior to other state-of-the-art IKSVM algorithms.
- Published
- 2019
33. Comparison the sensitivity of amphibian metamorphosis assays with NF 48 stage and NF 51 stage Xenopus laevis tadpoles
- Author
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Dong-Dong Jia, Yinfeng Zhang, Peifeng Li, Hong-Yu Yang, Fei Yu, and Hai-Ming Xu
- Subjects
endocrine system ,genetic structures ,Health, Toxicology and Mutagenesis ,Xenopus ,Amphibian metamorphosis ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Economic cooperation ,03 medical and health sciences ,Methimazole ,biology.animal ,parasitic diseases ,medicine ,0105 earth and related environmental sciences ,0303 health sciences ,biology ,urogenital system ,030302 biochemistry & molecular biology ,Thyroid ,Vertebrate ,biology.organism_classification ,Cell biology ,medicine.anatomical_structure ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
The amphibian metamorphosis assay (AMA) was proposed by the Organization for Economic Cooperation and Development (OECD) to screen thyroid disruptors of vertebrate species. The general experimental...
- Published
- 2019
34. Metabolic profiling study on potential toxicity in male mice treated with Dechlorane 602 using UHPLC-ESI-IT-TOF-MS
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Wuqun Tao, Zhiguang Zhou, Wanglong Zhang, Jun Yang, Chao Ma, Yangsheng Chen, Songyan Zhang, Xuejiao Yin, Heidi Qunhui Xie, Jijing Tian, Tuan Xu, Li Xu, Hualing Fu, Zhiling Guo, Bin Zhao, Feng Ji, and Hai-Ming Xu
- Subjects
Male ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,Phenylalanine ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Mass Spectrometry ,Mice ,chemistry.chemical_compound ,Metabolomics ,Kynurenic acid ,Hydrocarbons, Chlorinated ,Animals ,Polycyclic Compounds ,Xanthurenic acid ,Tyrosine ,Chromatography, High Pressure Liquid ,0105 earth and related environmental sciences ,Environmental Biomarkers ,Chemistry ,Tryptophan ,General Medicine ,Pollution ,Metabolic pathway ,Biochemistry ,Multivariate Analysis ,Pyrimidine metabolism ,Metabolome ,Environmental Pollutants ,Metabolic Networks and Pathways - Abstract
Dechlorane 602 (Dec 602), a chlorinated flame retardant, has been widely detected in different environmental matrices and biota. However, toxicity data for Dec 602 seldom have been reported. A metabolomics study based on ultra-high performance liquid chromatography coupled with ion trap time-of-flight mass spectrometry was employed to study the urine and sera metabolic profiles of mice administered with Dec 602 (0, 0.001, 0.1, and 10 mg/kg body weight per day) for 7 days. A significant difference in metabolic profiling was observed between the Dec 602 treated group and the control group by multivariate analysis, which directly reflected the metabolic perturbations caused by Dec 602. The metabolomics analyses of urine from Dec 602-exposed animals exhibited an increase in the levels of thymidine and tryptophan as well as a decrease in the levels of tyrosine, 12,13-dihydroxy-9Z-octadecenoic acid, 2-hydroxyhexadecanoic acid and cuminaldehyde. The metabolomics analyses of sera showed a decrease in the levels of kynurenic acid, daidzein, adenosine, xanthurenic acid and hypoxanthine from Dec 602-exposed animals. These findings indicated Dec 602 induced disturbance in phenylalanine, tyrosine and tryptophan biosynthesis, tryptophan metabolism, tyrosine metabolism, pyrimidine metabolism, purine metabolism, ubiquinone and other terpenoid-quinone biosynthesis; phenylalanine metabolism and aminoacyl-tRNA biosynthesis. Significant alterations of immune and neurotransmitter-related metabolites (tyrosine, tryptophan, kynurenic acid, and xanthurenic acid) suggest that the toxic effects of Dec 602 may contribute to its interactions with the immune and neuronal systems. This study demonstrated that the UHPLC-ESI-IT-TOF-MS-based metabolomic approach can obtain more specific insights into the potential toxic effects of Dec 602 at molecular level.
- Published
- 2019
35. Natural variation and evolutionary dynamics of transposable elements in Brassica oleracea based on next-generation sequencing data
- Author
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Jian-Hong Xu, Miao Fan, Zhen Liu, Hai-Ming Xu, Er-Kui Yue, Ming-Hua Duan, Yu Li, and Ruo-Fu Tao
- Subjects
0106 biological sciences ,0301 basic medicine ,Genome evolution ,Population ,Plant Science ,Horticulture ,Biology ,01 natural sciences ,Biochemistry ,Genome ,Article ,Mobile elements ,03 medical and health sciences ,Genetics ,Genetic variation ,Evolutionary dynamics ,education ,education.field_of_study ,Genetic diversity ,biology.organism_classification ,030104 developmental biology ,Evolutionary biology ,Brassica oleracea ,Mobilome ,Mobile genetic elements ,010606 plant biology & botany ,Biotechnology - Abstract
Brassica oleracea comprises various economically important vegetables and presents extremely diverse morphological variations. They provide a rich source of nutrition for human health and have been used as a model system for studying polyploidization. Transposable elements (TEs) account for nearly 40% of the B. oleracea genome and contribute greatly to genetic diversity and genome evolution. Although the proliferation of TEs has led to a large expansion of the B. oleracea genome, little is known about the population dynamics and evolutionary activity of TEs. A comprehensive mobilome profile of 45,737 TE loci was obtained from resequencing data from 121 diverse accessions across nine B. oleracea morphotypes. Approximately 70% (32,195) of the loci showed insertion polymorphisms between or within morphotypes. In particular, up to 1221 loci were differentially fixed among morphotypes. Further analysis revealed that the distribution of the population frequency of TE loci was highly variable across different TE superfamilies and families, implying a diverse expansion history during host genome evolution. These findings provide better insight into the evolutionary dynamics and genetic diversity of B. oleracea genomes and will potentially serve as a valuable resource for molecular markers and association studies between TE-based genomic variations and morphotype-specific phenotypic differentiation.
- Published
- 2020
36. Integrating transcriptome and metabolome reveals molecular networks involved in genetic and environmental variation in tobacco
- Author
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Jianfeng Shao, Zheng Qingxia, Shengchun Xu, Xu Guoyun, Niu Zhai, Gangjun Wang, Xin Lu, Jin Lifeng, Jie Luo, Cao Peijian, Pingping Liu, Xu Yalong, Guowang Xu, Hai-Ming Xu, Xusheng Wang, Qiansi Chen, and Huina Zhou
- Subjects
0106 biological sciences ,Nicotiana tabacum ,Metabolite ,Gene regulatory network ,Regulator ,Computational biology ,Genes, Plant ,tobacco ,01 natural sciences ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,Genetics ,Metabolome ,Gene Regulatory Networks ,Molecular Biology ,Gene ,030304 developmental biology ,0303 health sciences ,Full Paper ,biology ,co-expression module ,Genetic Variation ,molecular network ,Genomics ,General Medicine ,biology.organism_classification ,Carotenoids ,Molecular network ,Biological Variation, Population ,chemistry ,omics integration ,010606 plant biology & botany - Abstract
Tobacco (Nicotiana tabacum) is one of the most widely cultivated commercial non-food crops with significant social and economic impacts. Here we profiled transcriptome and metabolome from 54 tobacco samples (2–3 replicates; n = 151 in total) collected from three varieties (i.e. genetic factor), three locations (i.e. environmental factor), and six developmental stages (i.e. developmental process). We identified 3,405 differentially expressed (DE) genes (DEGs) and 371 DE metabolites, respectively. We used quantitative real-time PCR to validate 20 DEGs, and confirmed 18/20 (90%) DEGs between three locations and 16/20 (80%) with the same trend across developmental stages. We then constructed nine co-expression gene modules and four co-expression metabolite modules , and defined seven de novo regulatory networks, including nicotine- and carotenoid-related regulatory networks. A novel two-way Pearson correlation approach was further proposed to integrate co-expression gene and metabolite modules to identify joint gene–metabolite relations. Finally, we further integrated DE and network results to prioritize genes by its functional importance and identified a top-ranked novel gene, LOC107773232, as a potential regulator involved in the carotenoid metabolism pathway. Thus, the results and systems-biology approaches provide a new avenue to understand the molecular mechanisms underlying complex genetic and environmental perturbations in tobacco.
- Published
- 2020
37. Studies on property of sample size and different traits for core collections based on genotypic values of cotton
- Author
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Jin, Hu, Jun, Zhu, and Hai-ming, Xu
- Published
- 2001
- Full Text
- View/download PDF
38. Preparation of ZnO from 2 D nanosheets to diverse 1 D nanorods and their structure, surface area, photocurrent, optical and photocatalytic properties by simple hydrothermal synthesis
- Author
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Ruifang Zhong, Zhao Liu, Yangyang Li, Hai Ming Xu, Tingzhi Liu, and Shuwang Duo
- Subjects
Photocurrent ,Materials science ,Mechanical Engineering ,Metals and Alloys ,Analytical chemistry ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,symbols.namesake ,Mechanics of Materials ,Materials Chemistry ,Photocatalysis ,symbols ,Hydrothermal synthesis ,Nanorod ,0210 nano-technology ,Luminescence ,Raman spectroscopy ,BET theory ,Wurtzite crystal structure - Abstract
Two–dimensional (2 D) ZnO nanosheets and one–dimensional (1 D) bud−like, grenade−like, prism−like, bamboo−like, brush−like, arrow−like, pencil−like, drip pipe−like, baseball bat−like, gear−like, walking stick−like, taper−like, shuttle−like, and/or hollow tower−like ZnO nanorods (nanotubes) with various tips were synthesized by a simple hydrothermal route from the system of CO(NH2)2 N2H4. All the samples exhibit the wurtzite structure of ZnO. With the increase of the amount of N2H4 or decrease of CO(NH2)2, the interplanar spacing of different planes of ZnO increases or decreases simultaneously. FTIR shows that except the nanosheets of S1, all other samples are pure. The average reflectance of S8 reaches about 85% in the wavelengths ranging from 450 to 550 nm, and the reflectance of S3 is about 75%. The Eg values of ZnO from S1–S8 are 3.234, 3.215, 3.223, 3.215, 3.230, 3.243, 3.230 and 3.234 eV respectively. Raman spectra of S1 and S2 are obviously different from those of S3–S8. PL spectra of the ZnO samples exhibit a dominant UV luminescence peak of S1–S7, especially that of S1, S2 and S4. The addition of N2H4 can suppress the defects on the ZnO surface. However, the decrease of CO(NH2)2 introduces the defect. The BET surface area shows that except S1 (55.3 m2/g), S5 is a center of symmetric distribution of surface areas of S2–S8. From above discussion, the density, PL intensity of UV emission, and BET surface area of the ZnO nanorods can be easily tuned by controlling the concentrations of N2H4 and CO(NH2)2. The average values of photocurrent density of S1−S8 are 0.059, 0.170, 0.137, 0.184, 0.215, 0.091, 0.465 and 0.152 μA/cm2, respectively. S4, S2 and S7 possess the rapid photocatalytic activities and decompose 65%, 55% and 50% of MB within 50 min respectively. The order of the average diameters of S3, S4, S5, S6, and S7 agrees with that of photocatalytic efficiency, indicating that the polar (002) plane and/or the nanorod tips affect the photocatalytic activity. The decrease of the amount of CO(NH2)2 introduces the defects, which affects the photocatalytic activity of S8.
- Published
- 2017
39. TA Assisted Synthesis and Properties of Porous ZnO
- Author
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Shuwang Duo, Yangyang Li, Ting Zhi Liu, Rui Fang Zhong, and Hai Ming Xu
- Subjects
Materials science ,Nanostructure ,Mechanical Engineering ,Hydrazine ,Nanotechnology ,Alkali metal ,Hydrothermal circulation ,Absorbance ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Mechanics of Materials ,Tartaric acid ,Hydrothermal synthesis ,General Materials Science ,Wurtzite crystal structure - Abstract
Multihole ZnO hierarchical architectures were prepared by tartaric acid assisted hydrothermal synthesis, TA acts as a capping agent and structure-directing agent during the synthesis. Hydrazine acts as alkali source. XRD confirmed that that all ZnO possess the hexagonal wurtzite structure, and the crystalline of the sample turns better after annealed. The PL spectra can be tuned by changing TA concentration. The FTIR spectra of ZnO nanostructures shows the main absorbance peaks of all samples are located at about 547 cm−1, which are attributed to the vibrations of elongation of ZnO without any other bonds.
- Published
- 2017
40. Comparison the sensitivity of amphibian metamorphosis assays with NF 48 stage and NF 51 stage
- Author
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Yin-Feng, Zhang, Hai-Ming, Xu, Fei, Yu, Hong-Yu, Yang, Dong-Dong, Jia, and Pei-Feng, Li
- Subjects
Xenopus laevis ,Larva ,Toxicity Tests ,Metamorphosis, Biological ,Thyroid Gland ,Animals ,Biological Assay ,Endocrine Disruptors ,Sensitivity and Specificity ,Water Pollutants, Chemical - Abstract
The amphibian metamorphosis assay (AMA) was proposed by the Organization for Economic Cooperation and Development (OECD) to screen thyroid disruptors of vertebrate species. The general experimental design of the AMA exposes Nieuwkoop and Faber (NF) stage 51
- Published
- 2019
41. Cardiovascular toxicity and mechanism of bisphenol A and emerging risk of bisphenol S
- Author
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Xiao-Dan Hao, Li-Li Qian, Chan Shan, Dong-Dong Jia, Yinfeng Zhang, Hai-Ming Xu, Yu Wang, and Yi-Fei Zhang
- Subjects
endocrine system ,Bisphenol A ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,010501 environmental sciences ,medicine.disease_cause ,Bioinformatics ,01 natural sciences ,chemistry.chemical_compound ,Phenols ,In vivo ,medicine ,Animals ,Humans ,Environmental Chemistry ,Sulfones ,Epigenetics ,Benzhydryl Compounds ,Waste Management and Disposal ,0105 earth and related environmental sciences ,urogenital system ,business.industry ,Mechanism (biology) ,Lipid metabolism ,Pollution ,chemistry ,Bisphenol S ,Animal studies ,business ,hormones, hormone substitutes, and hormone antagonists ,Oxidative stress - Abstract
Epidemiological and animal studies indicate that increased exposure to bisphenol A (BPA) induces various human cardiovascular diseases (CVDs), including myocardial infarction, arrhythmias, dilated cardiomyopathy, atherosclerosis, and hypertension. Bisphenol S (BPS), an alternative to BPA, is increasingly present in various consumer products and human bodies worldwide. Recently, emerging evidence has shown that BPS might be related to cardiovascular disorders. In this review, we present striking evidence of the correlation between BPA exposure and various CVDs, and show that a nonmonotonic dose-response curve (NMDRC) was common in studies of the CV effects of BPA in vivo. The CV impairment induced by low doses of BPA should be highlighted, especially during developmental exposure or during coexposure with other risk factors. Furthermore, we explored the possible underlying mechanisms of these effects-particularly nuclear receptor signaling, ion channels, and epigenetic mechanisms-and the possible participation of lipid metabolism, oxidative stress and cell signaling. As the potential risks of BPA exposure in humans are still noteworthy, studies of BPA in CVDs should be strengthened, especially with respect to the mechanisms, prevention and treatment. Moreover, the potential CV risk of BPS reported by in vivo studies calls for immediate epidemiological investigations and animal studies to reveal the relationships of BPS and other BPA alternatives with human CVDs.
- Published
- 2020
42. Acetylcholinesterase Is a Potential Biomarker for a Broad Spectrum of Organic Environmental Pollutants
- Author
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Li Xu, Hai-Ming Xu, Hualing Fu, Yangsheng Chen, Yingjie Xia, Tuan Xu, Bin Zhao, Heidi Qunhui Xie, and Zhiling Guo
- Subjects
0301 basic medicine ,010501 environmental sciences ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Broad spectrum ,Environmental Chemistry ,Animals ,Pesticides ,0105 earth and related environmental sciences ,Pollutant ,Pesticide residue ,Organophosphate ,General Chemistry ,Pesticide ,Acetylcholinesterase ,030104 developmental biology ,Carbamate pesticides ,chemistry ,Potential biomarkers ,Environmental chemistry ,Environmental Pollutants ,Biomarkers ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Acetylcholinesterase (AChE, EC 3.1.1.7) is a classical biomarker for monitoring contamination and intoxication of organophosphate (OP) and carbamate pesticides. In addition to these classical environmental AChE inhibitors, other organic toxic substances have been found to alter AChE activity in various species. These emerging organic AChE disruptors include certain persistent organic pollutants (POPs), polycyclic aromatic hydrocarbons (PAHs), and wildly used chemicals, most of which have received considerable public health concern in recent years. It is necessary to re-evaluate the environmental significances of AChE in terms of these toxic substances. Therefore, the present review is aiming to summarize correlations of AChE activity of certain organisms with the level of the contaminants in particular habitats, disruptions of AChE activity upon treatment with the emerging disruptors in vivo and in vitro, and action mechanisms underlying the effects on AChE. Over 40 chemicals belonging to six main categories were reviewed, including 12 POPs listed in the Stockholm Convention. AChE activity in certain organisms has been found to be well correlated with the contamination level of certain persistent pesticides and PAHs in particular habitats. Moreover, it has been documented that most of the listed toxic chemicals could inhibit AChE activity in diverse species ranging from invertebrates to mammals. Besides directly inactivating AChE, the mechanisms in terms of interference with the biosynthesis have been recognized for some emerging AChE disruptors, particularly for dioxins. The collected evidence suggests that AChE could serve as a potential biomarker for a diverse spectrum of organic environmental pollutants.
- Published
- 2018
43. Design and Strength Analysis of a Guttering Plough with Two Adjusting Stages
- Author
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Zheng-Yong Zhang, Hai-ming Xu, Heng-Hui Sun, Yang Liu, and En-Wei Chen
- Subjects
Plough ,business.product_category ,business.industry ,Position (vector) ,Constraint (computer-aided design) ,Stress–strain curve ,Ansys software ,Structural engineering ,Boundary value problem ,business ,Finite element method ,Communication channel ,Mathematics - Abstract
A guttering plough with two adjusting stages was designed in this paper, which can adjust its configure and position in two stages. With these two adjusting movements, the ploughshare of this guttering plough can be put in soil at a deep position, to dig out a deeper channel than normal ones. The boundary conditions including loading and constraint were obtained, when it work normally. Combined with it, and the transformed configure and position in two stages, and the structure and loading characteristics of the guttering plough, a mechanical model was established with finite element analysis method. Then its analysis and solution were carried out by using ANSYS software. Under normal working condition, the stress and strain distributions of each part of this guttering plough were obtained. The calculation results showed that the structure of this guttering plough can meet the requirement of strength, but the positions of the joints were slightly poor, so it should be properly enlarged. Finite element software can be used to analyze the spatial static of this guttering plough, which can be certified as a feasible method for the design and improvement of the plow frame structure.
- Published
- 2018
44. Crosstalk between MicroRNAs and Peroxisome Proliferator-Activated Receptors and Their Emerging Regulatory Roles in Cardiovascular Pathophysiology
- Author
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Fei Yu, Yinfeng Zhang, Peifeng Li, Tao Xu, Mengyang Li, Jianxun Wang, Hai-Ming Xu, Man Wang, and Yan-Yan Gao
- Subjects
0301 basic medicine ,Cell growth ,business.industry ,Macrophage polarization ,Adipose tissue ,Review Article ,Bioinformatics ,03 medical and health sciences ,Crosstalk (biology) ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,lcsh:Biology (General) ,Adipogenesis ,Adipocyte ,Drug Discovery ,microRNA ,Medicine ,Pharmacology (medical) ,business ,Receptor ,lcsh:QH301-705.5 - Abstract
Peroxisome proliferator-activated receptors (PPARs) play vital roles in cardiovascular pathophysiology, such as energy balance, cell proliferation/apoptosis, inflammatory response, and adipocyte differentiation. These vital roles make PPARs potential targets for therapeutic prevention of cardiovascular diseases (CVDs). Emerging evidence indicates that the crosstalk of microRNAs (miRNAs) and PPARs contributes greatly to CVD pathogenesis. PPARs are inhibited by miRNAs at posttranscriptional mechanisms in the progress of pulmonary hypertension and vascular dysfunction involving cell proliferation/apoptosis, communication, and normal function of endothelial cells and vascular smooth muscle cells. In the development of atherosclerosis and stroke, the activation of PPARs could change the transcripts of target miRNA through miRNA signalling. Furthermore, the mutual regulation of PPARs and miRNAs involves cell proliferation/apoptosis, cardiac remodeling, and dysfunction in heart diseases. In addition, obesity, an important cardiovascular risk, is modulated by the regulatory axis of PPARs/miRNAs, including adipogenesis, adipocyte dysfunction, insulin resistance, and macrophage polarization in adipose tissue. In this review, the crosstalk of PPARs and miRNAs and their emerging regulatory roles are summarized in the context of CVDs and risks. This provides an understanding of the underlying mechanism of the biological process related to CVD pathophysiology involving the interaction of PPARs and miRNAs and will lead to the development of PPARs/miRNAs as effective anti-CVD medications.
- Published
- 2018
45. Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata
- Author
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Wu-Ji Wei, Yinfeng Zhang, Hai-Ming Xu, Qin-Qin Lou, Ya-Xian Zhao, Dongkai Ren, and Zhanfen Qin
- Subjects
Agonist ,medicine.medical_specialty ,DNA, Complementary ,Environmental Engineering ,Ranidae ,medicine.drug_class ,media_common.quotation_subject ,Molecular Sequence Data ,Xenopus ,Internal medicine ,medicine ,Animals ,Environmental Chemistry ,Amino Acid Sequence ,Cloning, Molecular ,Metamorphosis ,Receptor ,DNA Primers ,General Environmental Science ,media_common ,Receptors, Thyroid Hormone ,Thyroid hormone receptor ,Base Sequence ,Sequence Homology, Amino Acid ,biology ,Thyroid ,Metamorphosis, Biological ,Gene Expression Regulation, Developmental ,General Medicine ,biology.organism_classification ,Endocrinology ,medicine.anatomical_structure ,Hormone ,Rana nigromaculata - Abstract
Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone (TH) signaling disrupting actions. To validate the utilizability of R. nigromaculata, we investigated the responsiveness of R. nigromaculata to a TH receptor (TR) agonist (T3) and antagonist (amiodarone) by analyzing expression, based on characterizing TR cDNA and developmental expression patterns. With high levels of identity with the corresponding genes in X. laevis, both TRα and TRβ in R. nigromaculata exhibited roughly similar developmental expression patterns to those of X. laevis, in spite of some species-specific differences. Both TRα and TRβ expression had greater changes in the liver and intestine than in the tail and brain during metamorphosis. T3 exposure for 2days induced more dramatic increases of TRβ expression in stage 27 than in stage 34 tadpoles but not in stage 42 tadpoles, showing that the responsiveness of R. nigromaculata to TH decreased with development and disappeared at the onset of metamorphic climax. Corresponding to greater changes of TRβ expression in the liver and intestine than in the tail and brain during metamorphosis, the liver and intestine had higher responsiveness to exogenous T3 than the tail and brain. Amiodarone inhibited T3-induced TRβ expression. Our results show that R. nigromaculata can be used as a model species for assaying TH signaling disrupting actions by analyzing TRβ expression, and intestine tissues at stage 27 are ideal test materials due to high responsiveness and easy accessibility.
- Published
- 2014
46. Role of short-wavelength filtering lenses in delaying myopia progression and amelioration of asthenopia in juveniles
- Author
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Jin Jiang, Hai-Lan Zhao, Jie Yu, and Hai-Ming Xu
- Subjects
0301 basic medicine ,medicine.medical_specialty ,genetic structures ,Test group ,media_common.quotation_subject ,Optical power ,short-wavelength filtering lenses ,juvenile myopia ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Ophthalmology ,Clinical Research ,Ophthalmology ,medicine ,Contrast (vision) ,media_common ,business.industry ,asthenopia ,Glare (vision) ,Axial length ,eye diseases ,Amplitude of accommodation ,030104 developmental biology ,lcsh:RE1-994 ,030221 ophthalmology & optometry ,Optometry ,sense organs ,Accommodative lag ,business - Abstract
AIM: To evaluate the positive effects of blue-violet light filtering lenses in delaying myopia and relieving asthenopia in juveniles. METHODS: Sixty ametropia juveniles (aged range, 11-15y) were randomized into two groups: the test group (30 children, 60 eyes), wearing blue-violet light filtering lenses; and the control group (30 children, 60 eyes), wearing ordinary aspherical lenses. Baseline refractive power of the affected eyes and axial length of the two groups was recorded. After 1-year, the patients underwent contrast sensitivity (glare and non-glare under bright and dark conditions), accommodation-related testing, asthenopia questionnaire assessment, and adverse reaction questionnaire assessment. RESULTS: After 1y of wearing the filtering lenses, changes in refractive power and axial length were not significantly different between the two groups (P>0.05). Under bright conditions, the contrast sensitivities at low and medium-frequency grating (vision angles of 6.3°, 4.0°, and 2.5°) with glare in the test group were significantly higher than in the control group (P0.05). In the test group, the amplitude of accommodation, accommodative lag, and accommodative sensitivity of patients wearing glasses for 6 and 12mo were significantly elevated (P0.05), and the asthenopia grating was not significantly decreased (P>0.05). In addition, after wearing glasses for 6 to 12mo, the asthenopia grating of patients in the test group decreased significantly compared with the control group (P0.05). CONCLUSION: A 1-year follow-up reveal that compare with ordinary glasses, short-wavelength filtering lenses (blue/violet-light filters) increase the low- and medium-frequency contrast sensitivity under bright conditions and improved accommodation. They effectively relieved asthenopia without severe adverse reactions, suggesting potential for clinical application. However, no significant advantages in terms of refractive power or axial length progression were found compared with ordinary aspheric lenses.
- Published
- 2017
47. Multiple Indefinite Kernel Learning for Feature Selection
- Author
-
Hui Xue, Yu Song, and Hai-Ming Xu
- Subjects
0209 industrial biotechnology ,Graph kernel ,Information Systems and Management ,Optimization problem ,Computer science ,Feature selection ,Positive-definite matrix ,02 engineering and technology ,Management Information Systems ,Kernel (linear algebra) ,020901 industrial engineering & automation ,Artificial Intelligence ,Polynomial kernel ,020204 information systems ,0202 electrical engineering, electronic engineering, information engineering ,Multiple kernel learning ,business.industry ,Pattern recognition ,Support vector machine ,Kernel method ,Feature (computer vision) ,Kernel embedding of distributions ,Norm (mathematics) ,Kernel (statistics) ,Radial basis function kernel ,020201 artificial intelligence & image processing ,Affine transformation ,Artificial intelligence ,Tree kernel ,Convex function ,business ,Algorithm ,Software - Abstract
Multiple kernel learning for feature selection (MKL-FS) utilizes kernels to explore complex properties of features and performs better in embedded methods. However, the kernels in MKL-FS are generally limited to be positive definite. In fact, indefinite kernels often emerge in actual applications and can achieve better empirical performance. But due to the non-convexity of indefinite kernels, existing MKL-FS methods are usually inapplicable and the corresponding research is also relatively little. In this paper, we propose a novel multiple indefinite kernel feature selection method (MIK-FS) based on the primal framework of indefinite kernel support vector machine (IKSVM), which applies an indefinite base kernel for each feature and then exerts an l 1 -norm constraint on kernel combination coefficients to select features automatically. A two-stage algorithm is further presented to optimize the coefficients of IKSVM and kernel combination alternately. In the algorithm, we reformulate the non-convex optimization problem of primal IKSVM as a difference of convex functions (DC) programming and transform the non-convex problem into a convex one with the affine minorization approximation. We further utilize a leverage score sampling method to select landmark points for solving large-scale problems. Moreover, we extend MIK-FS to multi-class feature selection scenarios. Experiments on real-world datasets demonstrate that MIK-FS is superior to some related state-of-the-art methods in both feature selection and classification performance.
- Published
- 2017
48. Solving Indefinite Kernel Support Vector Machine with Difference of Convex Functions Programming
- Author
-
Hai-Ming Xu, Hui Xue, Xiao-Hong Chen, and Yun-Yun Wang
- Subjects
General Medicine - Abstract
Indefinite kernel support vector machine (IKSVM) has recently attracted increasing attentions in machine learning. Different from traditional SVMs, IKSVM essentially is a non-convex optimization problem. Some algorithms directly change the spectrum of the indefinite kernel matrix at the cost of losing some valuable information involved in the kernels so as to transform the non-convex problem into a convex one. Other algorithms aim to solve the dual form of IKSVM, but suffer from the dual gap between the primal and dual problems in the case of indefinite kernels. In this paper, we directly focus on the non-convex primal form of IKSVM and propose a novel algorithm termed as IKSVM-DC. According to the characteristics of the spectrum for the indefinite kernel matrix, IKSVM-DC decomposes the objective function into the subtraction of two convex functions and thus reformulates the primal problem as a difference of convex functions (DC) programming which can be optimized by the DC algorithm (DCA). In order to accelerate convergence rate, IKSVM-DC further combines the classical DCA with a line search step along the descent direction at each iteration. A theoretical analysis is then presented to validate that IKSVM-DC can converge to a local minimum. Systematical experiments on real-world datasets demonstrate the superiority of IKSVM-DC compared to state-of-the-art IKSVM related algorithms.
- Published
- 2017
49. A New Species of the GenusSynersagaGozmány (Lepidoptera, Lecithoceridae) From China
- Author
-
Hai-Ming Xu, Min Wang, and Houshuai Wang
- Subjects
Entomology ,South china ,biology ,media_common.quotation_subject ,Juxta ,Insect ,Anatomy ,biology.organism_classification ,Lecithoceridae ,Lepidoptera genitalia ,Insect Science ,Synersaga ,Taxonomy (biology) ,Ecology, Evolution, Behavior and Systematics ,media_common - Abstract
A new species of the genus Synersaga Gozmany, Synersaga atriptera Xu & Wang sp. nov. (Lepidoptera: Lecithoceridae) is described from China. Adults, wing venation and male genitalia are illustrated. The new species is similar to S. nigriptera, but is distinguished by the wing venation and the male genitalia. The ground color of wings of S. atriptera sp. nov. is darker than that of the wings of S. nigriptera, especially the hind wings; the forewing of S. atriptera sp. nov. has a broad and blackish transverse outer line, which it is not present in S. nigriptera; and the fringe of S. nigriptera has a paler basal line, which it is not obvious in S. atriptera sp. nov. Juxta of S. atriptera sp. nov. has a pair of claviform lateral lobes, which are separated at the base. Juxta of S. nigriptera also has a pair of claviform lateral lobes, but they are connected at the base by a heavily sclerotized band. Type specimens were deposited in the Insect Collection of the Department of Entomology, South China Agricultural University, Guangzhou, China.
- Published
- 2014
50. Effects of Zinc on Hepatopancreatic Cell Culture of Kuruma Prawn, Litopenaeus vannamei
- Author
-
Da Li, Hai Ming Xu, Hui Wang, Chun Long Zhao, Hong Wei Wang, and Duan Bo Cai
- Subjects
biology ,Cell division ,Chemistry ,General Engineering ,Litopenaeus ,biology.organism_classification ,Molecular biology ,Penicillin ,Cell culture ,medicine ,Prawn ,Hepatopancreas ,Subculture (biology) ,Primary cell ,medicine.drug - Abstract
The hepatopancreatic cell culture of the kuruma prawn, Litopenaeusvannamei, was conducted to identify the effects of zinc on cell division. The culturesystem consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl,1.011g/L glucose, 1000 UI/ml penicillin, 1000 μg/ml treptomycin, 20% heatinactivated fetal calf serum (FCS) for primary cells and 10 % for subculture cells. TheRNA/DNA ratio in cultured cells was measured. The results show that the celldivision of cultured hepatopancreas cells in L. vannamei was increased by the optimalconcentration of Zn2+, 80 μg/L.
- Published
- 2013
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