9 results on '"Hai-yong He"'
Search Results
2. Nonoxid-HMGB1 Attenuates Cognitive Impairment After Traumatic Brain Injury in Rats
- Author
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Jun-Quan Chen, Shuang-Qi Gao, Lun Luo, Zong-Yuan Jiang, Chao-Feng Liang, Hai-Yong He, and Ying Guo
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cognitive impairment ,redox state ,HMGB1 ,traumatic brain injury ,SH3RF2 ,Medicine (General) ,R5-920 - Abstract
Traumatic brain injury (TBI) is a major global burden of health. As an accepted inflammatory mediator, high mobility group box 1 (HMGB1) is found to be effective in facilitating neurogenesis and axonal regeneration. SH3RF2 (also known as POSHER), an E3 ligase SH3 domain-containing ring finger 2, belongs to the SH3RF family of proteins. Here, we aimed to investigate the role of redox states of HMGB1 on neurite outgrowth and regeneration both in vitro and in vivo. In this study, distinct recombinant HMGB1 redox isoforms were used. Sequencing for RNA-seq and data analysis were performed to find the potential downstream target of nonoxid-HMGB1 (3S-HMGB1). Protein changes and distribution of SH3RF2 were evaluated by western blot assays and immunofluorescence. Lentivirus and adeno-associated virus were used to regulate the expression of genes. Nonoxid-HMGB1-enriched exosomes were constructed and used to treat TBI rats. Neurological function was evaluated by OF test and NOR test. Results demonstrated that nonoxid-HMGB1 and fr-HMGB1, but not ds-HMGB1, promoted neurite outgrowth and axon elongation. RNA-seq and western blot assay indicated a significant increase of SH3RF2 in neurons after treated with nonoxid-HMGB1 or fr-HMGB1. Notably, the beneficial effects of nonoxid-HMGB1 were attenuated by downregulation of SH3RF2. Furthermore, nonoxid-HMGB1 ameliorated cognitive impairment in rats post-TBI via SH3RF2. Altogether, our experimental results suggest that one of the promoting neurite outgrowth and regeneration mechanisms of nonoxid-HMGB1 is mediated through the upregulated expression of SH3RF2. Nonoxid-HMGB1 is an attractive therapeutic candidate for the treatment of TBI.
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- 2022
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3. Retraction: Glioma-Derived ADAM10 Induces Regulatory B Cells to Suppress CD8+ T Cells.
- Author
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Zhuo-Peng Ye, Hai-Yong He, Hui Wang, Wen-Sheng Li, Lun Luo, Zhen-Chao Huang, Ying Guo, and PLOS ONE Editors
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Medicine ,Science - Published
- 2021
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4. Glioma-derived ADAM10 induces regulatory B cells to suppress CD8+ T cells.
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Zhuo-peng Ye, Hai-yong He, Hui Wang, Wen-sheng Li, Lun Luo, Zhen-chao Huang, and Ying Guo
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Medicine ,Science - Abstract
CD8+ T cells play an important role in the anti-tumor activities of the body. The dysfunction of CD8+ T cells in glioma is unclear. This study aims to elucidate the glioma cell-derived ADAM10 (A Disintegrin and metalloproteinase domain-containing protein 10) in the suppression of CD8+ effector T cells by the induction of regulatory B cells. In this study, glioma cells were isolated from surgically removed glioma tissue and stimulated by Phorbol myristate acetage (PMA) in the culture. The levels of ADAM10 in the culture were determined by enzyme-linked immunosorbent assay. Immune cells were assessed by flow cytometry. The results showed that the isolated glioma cells express ADAM10, which was markedly up regulated after stimulated with PMA. The glioma-derived ADAM10 induced activated B cells to differentiate into regulatory B cells, the later suppressed CD8+ T cell proliferation as well as the induced regulatory T cells, which also showed the immune suppressor effect on CD8+ effector T cell proliferation. In conclusion, glioma cells produce ADAM10 to induce Bregs; the latter suppresses CD8+ T cells and induces Tregs.
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- 2014
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5. Endoscopic anatomical study of the trans-lateral molar approach to the infratemporal fossa
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Wei-Wei Cai, Hai-Yong He, Zhuang Kang, Qintai Yang, Yan Zou, and Jian-Gang Liang
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medicine.medical_specialty ,Endoscope ,Skull Base Neoplasms ,03 medical and health sciences ,0302 clinical medicine ,Cadaver ,medicine ,Humans ,030223 otorhinolaryngology ,Aged ,Pterygopalatine fossa ,business.industry ,Infratemporal fossa ,Temporal Bone ,Endoscopy ,General Medicine ,Neurovascular bundle ,Molar ,Skull ,medicine.anatomical_structure ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Female ,Radiology ,Neurosurgery ,business ,Neurilemmoma - Abstract
The infratemporal fossa (ITF) is located deep in the skull base. Recently, the endoscopic transoral approach has enabled maxillofacial surgeons to access the ITF using a less invasive approach compared to the traditional transfacial and endonasal endoscopic approaches. The present study aims to provide maxillofacial surgeons with new data concerning direct endoscopic measurement and precise anatomical topography features of the endoscopic trans-lateral molar approach to ITF by comparing the endoscopic and regional anatomy of ITF. A clinical case receiving the proposed surgical approach is used to determine the feasibility of this technique. The anatomical data were obtained by measuring the bone anatomical landmarks and analyzing the CT imaging data using GE’s Advance Windows 4.1 software on 25 subjects (50 sides). Morphological pictures of the regional anatomy and endoscopic anatomy were obtained from 6 (12 sides) adult cadaver heads, and the anatomical features were described. The present study reports the management of one case using the proposed surgical approach. The proposed surgical approach clearly revealed neurovascular, muscular, and surgical landmarks in the ITF. The surgical case supports the minimally invasive treatment approach, which could rapidly access the ITF and completely excise benign tumors. The anatomical studies and surgical case presentation helps us understand the spatial relationship of surgical landmarks of the surgical approach to the ITF for the treatment of benign lesions in the deep cranial base area.
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- 2019
6. Intracranial lymphatic drainage discharges iron from the ventricles and reduce the occurrence of chronic hydrocephalus after intraventricular hemorrhage in rats
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Wang Bocheng, Liang Chaofeng, Gao Qun, Chen Chuan, Hai-yong He, Guo Ying, and Huang Tengchao
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0301 basic medicine ,Male ,Pathology ,medicine.medical_specialty ,Iron ,Blotting, Western ,Autologous blood injection ,Cerebral Ventricles ,Lymphatic System ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Animals ,Cerebral Intraventricular Hemorrhage ,biology ,business.industry ,General Neuroscience ,General Medicine ,medicine.disease ,Magnetic Resonance Imaging ,Hydrocephalus ,Rats ,Ferritin ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Lymphatic system ,Intraventricular hemorrhage ,Ventricle ,Deep cervical lymph nodes ,Ferritins ,Cerebrospinal fluid circulation ,biology.protein ,Lymph ,business ,030217 neurology & neurosurgery - Abstract
Background: The discovery of intracranial lymphatic system provides a new theory about cerebrospinal fluid circulation. In order to investigate the role of lymphatic drainage in hydrocephalus after intraventricular hemorrhage (IVH), three parts of experiments were performed.Methods: IVH model was produced by 200ul autologous blood injection into lateral ventricle of SD male rats. Perls Prussian blue reaction was used to confirm the ability of iron drainage through deep cervical lymph nodes (DCLNs) in part I. The part II measured the volume of ventricles by MR scanner , and the part III compared the ferritin level of area surrounding ventricles by Western blotting after IVH.Results: The ability of iron drainage through DCLNs was confirmed. DCLNs-excised rats got a 19.6% higher incidence of hydrocephalus than DCLNs-preserved rats at day 28 after IVH. And DCLNs-excised group showed higher ferritin level than DCLNs-preserved group at day 3 after IVH.Conclusion: The whole experiments revealed the role of intracranial lymphatic system in discharging iron out of central nervous system at acute phase of IVH, and proved the function in alleviating and avoiding hydrocephalus at chronic phase after IVH.
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- 2019
7. MicroRNA-195 inhibits the proliferation of human glioma cells by directly targeting cyclin D1 and cyclin E1
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Lu Yuntao, Wang Hui, Ba Yueyang, Hai-yong He, Luo Lun, Li Wensheng, and Liang Chaofeng
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Cyclin E ,Tumor Physiology ,Cyclin D ,Science ,Cyclin A ,Cyclin B ,Down-Regulation ,Cell Growth ,Mice ,Cyclin D1 ,Cell Line, Tumor ,Proliferating Cell Nuclear Antigen ,Glioma ,Molecular Cell Biology ,Basic Cancer Research ,Biomarkers, Tumor ,medicine ,Animals ,Humans ,Biology ,Neurological Tumors ,3' Untranslated Regions ,Cell Proliferation ,Oncogene Proteins ,Mice, Inbred BALB C ,Multidisciplinary ,Base Sequence ,biology ,Cancers and Neoplasms ,medicine.disease ,Cell biology ,MicroRNAs ,Cyclin E1 ,Cell Transformation, Neoplastic ,Oncology ,Astrocytes ,biology.protein ,Cancer research ,Medicine ,Cyclin A2 ,Research Article - Abstract
Glioma proliferation is a multistep process during which a sequence of genetic and epigenetic alterations randomly occur to affect the genes controlling cell proliferation, cell death and genetic stability. microRNAs are emerging as important epigenetic modulators of multiple target genes, leading to abnormal cellular signaling involving cellular proliferation in cancers.In the present study, we found that expression of miR-195 was markedly downregulated in glioma cell lines and human primary glioma tissues, compared to normal human astrocytes and matched non-tumor associated tissues. Upregulation of miR-195 dramatically reduced the proliferation of glioma cells. Flow cytometry analysis showed that ectopic expression of miR-195 significantly decreased the percentage of S phase cells and increased the percentage of G1/G0 phase cells. Overexpression of miR-195 dramatically reduced the anchorage-independent growth ability of glioma cells. Furthermore, overexpression of miR-195 downregulated the levels of phosphorylated retinoblastoma (pRb) and proliferating cell nuclear antigen (PCNA) in glioma cells. Conversely, inhibition of miR-195 promoted cell proliferation, increased the percentage of S phase cells, reduced the percentage of G1/G0 phase cells, enhanced anchorage-independent growth ability, upregulated the phosphorylation of pRb and PCNA in glioma cells. Moreover, we show that miR-195 inhibited glioma cell proliferation by downregulating expression of cyclin D1 and cyclin E1, via directly targeting the 3'-untranslated regions (3'-UTR) of cyclin D1 and cyclin E1 mRNA. Taken together, our results suggest that miR-195 plays an important role to inhibit the proliferation of glioma cells, and present a novel mechanism for direct miRNA-mediated suppression of cyclin D1 and cyclin E1 in glioma.
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- 2013
8. Correlation of MMP1 and TIMP1 expression with pituitary adenoma fibrosis.
- Author
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Hui Wang, Wen-Sheng Li, De-Jin Shi, Zhuo-Peng Ye, Feng Tai, Hai-Yong He, Chao-Feng Liang, Jin Gong, and Ying Guo
- Abstract
Abstract  Objective To explore the correlation of matrix metalloproteinase-1 (MMP1) and tissue inhibitor of metalloproteinase-1 (TIMP1) with pituitary adenoma fibrosis. Methods Thirty-eight pituitary adenoma specimens were divided into fibrous group (6 patients) and non-fibrous group (32 patients). MMP1 and TIMP1 expression was detected by RT-PCR and immunohistochemistry. The collagen content was determined by Sirius scarlet staining. Results In fibrous and non-fibrous group: (1) the collagen content was 20.95 ± 8.42% and 7.98 ± 5.18% respectively, and there was a statistical significance (P 1mRNA was 0.47 ± 0.40 and 0.59 ± 0.54 respectively and its protein expression was 0.12 ± 0.09 and 0.13 ± 0.09 respectively, and there was no statistical significance (P > 0.05). Their expression was not related to collagen content (P > 0.05). (3) The expression of TIMP1mRNA was 1.61 ± 1.09 and 0.79 ± 0.59 respectively and its protein expression was 0.58 ± 0.11 and 0.32 ± 0.18 respectively, and there was a statistical significance (P P Conclusion The pathological features of pituitary adenoma fibrosis are excessive collagen deposition. High expression of TIMP1 may be an important cause. [ABSTRACT FROM AUTHOR]
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- 2008
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9. Evaluation of ventriculoperitoneal shunt in the treatment of intracranial hypertension in the patients with cryptococcal meningitis: A report of 12 cases.
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Hui Wang, Cong Ling, Chuan Chen, Hai-yong He, Lun Luo, and Xin - jie Ning
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INTRACRANIAL hypertension , *MENINGITIS , *CRYPTOCOCCUS neoformans , *PATHOGENIC microorganisms , *IMMUNITY , *BLOOD diseases , *PATIENTS , *THERAPEUTICS - Published
- 2014
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