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Background: Patient/client management is comprehensive multidirectional process. It includes patient client examination, evaluation, diagnosis, prognosis, intervention and then discharge plans from supervised treatment to follow ups. The process of examination includes comprehensive history taking, performing systemic review and special tests and measures. Aim: To investigate Pakistani physiotherapists’ current practice, skills and understanding of evidence-based practice in order to improve the utilization of the evidence base in physiotherapy in the country. Methods: This observational cross section study was done in Pakistan Institute of Medical Sciences and Islamabad Medical and Dental College, Islamabad between January 2021 and March 2021. Physiotherapists(n=369) were contacted through non-probability convenience sampling technique. Respondents were contacted at clinical facilities in the twin cities of Pakistan. The content of the questionnaire was validated using the literature and the method of Lynn MR et al. The results were extracted, conclusion was drawn, suggestions and recommendations were made. Results: The participants mean age was 27.58±7.41 years. The male/female ratio was almost same. Approximately half of the respondents 50.4% were agreed about the role of EBP in practice.57% respondents were agreed that EBP removes the art from clinical practice. 58.5% respondents were agreed about benefit of EBP and EBP improves clinical outcomes and 53.4%respondents were agreed about help of EBP in decision making. Attitude and knowledge need analysis was positive but there found compromise in utilization of this evidence-based practice, while this study showed more utilization. Conclusion: Five steps of evidence-based practice were found being infrequently practiced. Physiotherapists had good to fair understanding of evidence-based practice. Physiotherapists had fair to poor appraisal skills. Keywords: Clinician practice; Data base; Evidence-based practice; Physical therapist; Practice guideline; Patient management.

The present investigation was carried out to explore the bioactive compounds from the n-hexane fraction of methanolic extract of quinoa (Chenopodium quinoa Willd.) roots. For this purpose, C. quinoa roots were collected, shade dried, and crushed into a fine powder. The powdered material was extracted in methanol, filtered, and the filtrate was partitioned with n-hexane, followed by GC-MS analysis of the n-hexane fraction. The quantitative determination of this fraction revealed the presence of 15 phytochemical constituents of diverse nature. Among these, octadec-9-enoic acid (44.18%); n-hexadecanoic acid (18.87%); methyl (Z)-octadec-9-enoate (12.87%); methyl hexadecanoate (4.30%); 2,3-dihydroxypropyl elaidate (3.63%); phthalic acid (3.08%); methyl octadecanoate (2.27%) and 1,12-tridecadiene (2.00%) were prevailing as the most abundant to moderately occurring compounds. A thorough literature survey was carried out to collect information regarding the pharmaceutical properties of the identified compounds. It showed some of the identified compounds namely dodecanoic acid; tetradecanoic acid; 2-benzoyl-d-galactosan; n-hexadecanoic acid; methyl octadecanoate; octadec-9-enoic acid; and 2,3-dihydroxypropyl elaidate possess antifungal, antibacterial, antioxidant, antiviral, anti-inflammatory, and/or anti-cancer properties.

This paper investigates the effect of students’ cognitive and emotional engagement on learning achievement in government primary male schools. Academic achievement was assessed through academic success and academic productivity. Data were gathered and utilized a survey questionnaire with 120 male primary school teachers as the sample. Descriptive Statistics and Pearson correlation were used to assess the data that had been obtained. Analysis of the data revealed a strong positive correlation between student cognitive engagement with and academic success and academic productivity. Based on the findings, the study concluded that educational stakeholders should concentrate on student engagement as a key factor that influences a distal outcome, such as increased academic production and accomplishment. The study recommended that educators, decision-makers, and the research community pay closer attention to and identify ways to improve student involvement. Additionally, educators should create and implement a variety of classroom activities that appeal to students' cognitive functions. The implications of the findings for academic programs, institutions, teachers, and students are far larger. They support primary educational institutions by providing important data for student involvement and academic success. Additionally, the findings open up fresh avenues and perspectives for further study.

Objective: To quantify the frequency of phantom limb pain, limb sensation and stump pain among amputees aging 20–75 years. Methods: An observational cross-sectional study was done. A population of 75 amputees were selected using non-probability convenient sampling and after setting the inclusion and exclusion criteria. Standard questionnaires; Groningen Questionnaire Problems after Arm Amputation (GQPAA) and Groningen Questionnaire Problems after Leg Amputation (GQPLA), were filled by interviewing the patients. All the patients were approached in the hospitals. Data was collected from Pakistan Institute of Medical Sciences, Islamabad and Fauji Foundation Hospital, Rawalpindi. The duration of study was 6 months from June 2021 to December 2021. Results: After compiling all the results from the questionnaires, it was unveiled that among 75 amputees, mean age was 43.12±11.43 years. Out of these amputees, 53.3% were upper limb amputees and 46.7% were lower limb amputees. the frequency of phantom sensation was 62.7%, 57.3% reported phantom limb pain and 84% experienced stump pain. Conclusion: The study concluded that most prevalent among the population was stump pain, which was followed by phantom limb sensations and the least prevalent of these three was phantom limb pain among the population.

Background and Aim: In today sports such as volleyball and basketball, Vertical Jump is considered as important component that enhance the performance of athlete. The aim of this study was to determine the effect of muscle energy technique on vertical jump performance in volleyball and basketball players. Methodology: This Randomized Clinical Trial was conducted at Islamic International University Islamabad. Twenty-nine athletes of age 18 – 35 playing basketball and volley as part time/domestic level were included. Post isometric relaxation technique was applied on group A (n=15) and static stretching was applied on group B (n=14). Surface electromyography activity of quadriceps and abdominal muscle was recorded, Stable time, airtime and vertical jump height were measured using two-axis force platform and vertical jump height was measured by motion sensor. Measurements were taken at baseline and immediately after applying interventions to both groups. Data entry and analysis were done by using software SPSS version 22. Results: Of the 29 athletes, 15 were in the group A and 14 were in the group B. Immediate assessment of vertical jump height was not significantly improved by post-isometric relaxation relative to static stretching (p=0.594). Muscle recruitment, ground reaction and vertical jump height improved apparently after post isometric relaxation but not significantly. Conclusion: It appears that post isometric relaxation and static stretching of quadriceps shows no significant difference in vertical jump height.

Aptamers are short strands of nucleic acid with a single strand that may unite to target a certain molecule in a selective and specific manner. SELEX experiments are the typical method used for identifying aptamers in vitro (systematic evolution of ligands by exponential enrichment). Several different computational methods have been developed to locate aptamers. The purpose of this research is to identify and make predictions on the possible RNA aptamers that may be used to target the protein. To do this, we propose the use of a multi-layer perceptron neural network with sixteen layers that are trained to locate possible aptamers of a protein target. This network is trained by extracting the main properties of RNA sequences. The outcome of our proposed model is compared to the output of two well-known machine learning classifiers, namely random forest (RF) and support vector machine (SVM). Additionally, we undertake the independent testing of our model on the benchmark dataset, which allows us to reach the highest accuracy possible. As a consequence of this, our model obtains an accuracy of 98.44% and an MCC of 0.9123 during the 15-fold cross-validation, and it achieves an accuracy of 98.10% and an MCC of 0.9354 when the leave-one-out cross-validation is performed. We are certain that our approach will contribute to a reduction in the amount of money and time spent on in vitro testing. Therefore, restricting the length of the initial pool of potential nucleic acid pattern combinations.

The composition of essential oil of marigold (Tagetes erecta L.) flowers was investigated. The oil was analyzed by GC-MS for identification of various components. Among these, 3-cyclohexene-1-methanol, α, α,4-trimethyl-, acetate was the most abundant compound (31.67%) followed by phenol, 2,4-bis (1,1- dimethylethyl)- (14.35%) and eucalyptol (14.27%). Other compounds included dodecanoic acid (5.97%), tetradecanoic acid (5.58%), octadecane (4.93%), propanoic acid, 2-bromo-2-methyl-, ethyl ester (5.43%), cyclohexanol, 1-methyl-4-(1-methylethanyl)-, acetate (5.33), n-hexadecanoic acid (4.70), p-menth-1-en-8-ol (4.20%) and thymol (3.57%). Bangladesh J. Bot. 51(4): 683-688, 2022 (December)

Macrophomina phaseolina is a highly problematic fungus that causes diseases in hundreds of plant species. In order to search for an alternative to chemical fungicides for the control of this devastating pathogen, leaf extract of Tabernaemontana divaricata was assessed for the control of M. phaseolina. Five concentrations of leaf extract in methanol viz. 1, 2, 3, 4 and 5% (w/v), were checked against M. phaseolina. All these concentrations significantly suppressed the fungal growth resulting in 34–74% decreased biomass of M. phaseolina over control. GC-MS analysis of this extract showed the presence of 54 compounds. Squalene was the predominant compound with 24.11% peak area followed by vitamin E (8.96%). Other important compounds were hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (6.21%), thiophene, tetrahydro-2-methyl- (5.68%), cyclopentanol (5.61%), neophytadiene (6.25%), 1-tert-butoxypropan-2-yl 2-methylbutanoate (6.03%), ibogamine-18-carboxylic acid, 12-methoxy-, methyl ester (2.95%), phytol (2.26%), and n-hexadecanoic acid (2.20%). Some of these major compounds might be responsible for antifungal property of methanolic leaf extract of T. divaricata against M. phaseolina.

The leaves of parthenium (Parthenium hysterophorus L.) were extracted in ethanol and toxicity of the extract was examined on khapra beetle (Trogoderma granarium Everts) larvae. Four concentrations (2, 4, 6 and 8 µL mL-1 acetone) were applied directly on the larvae in Petri dishes. Toxicity was recorded after 12, 24 and 36 h. All the concentrations had considerable effect on the mortality of larvae. At the highest concentration (8 µL mL-1), the mortality observed was 57%, 72% and 78% after 12, 24 and 36 h, respectively. The minimum mortality was observed to be 43% at the concentration of 2 µL mL-1 acetone after 12 h. GC-MS analysis of the parthenium leaves showed 7 compounds in the extract including phytol (38.68%), β-cubebene (11.98%), hexadecanoic acid, methyl ester (11.22%), caryophyllene (10.37%), 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (9.98%), methyl stearate (9.01%), and hexadecanoic acid, ethyl ester (8.74%). The study concluded that an extract of 8 µL mL-1 acetone concentration is highly effective against larvae of khapra beetles that can cause 78% mortality after 36 h of direct exposure to the larvae.

Coronavirus disease-2019 (COVID-19) is pro-inflammatory disorder characterized by acute respiratory distress syndrome. Interleukin-6, a cytokine secreted by macrophages, which mediates an inflammatory response, is frequently increased and associated with the severity in COVID-19 patients. The differential expression of IL6 cytokine in COVID-19 patients may be associated with the presence of single nucleotide polymorphisms (SNPs) in regulatory region of cytokine genes. The aim of this study is to investigate the role of two promoter polymorphisms of the IL6 gene (-597G A and -174G C) with the severity of COVID-19. The study included 242 patients, out of which 97 patients with severe symptoms and 145 patients with mild symptoms of COVID-19. Genotyping of two selected SNPs, rs1800795 (-174G C) and rs1800797 (-597G A) of promoter region of IL6 gene, was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In our study, individuals with GC genotypes of IL6 (-174G C) polymorphism showed significantly higher risk of severity [adjusted odds (OR) 3.86, p .001] but we did not observe any association of COVID-19 severity with rs1800797 (-597G A) polymorphism. The COVID-19 severity was significantly higher in individuals having 'C' allele of IL6 (-174G C) polymorphism (p = .014). Linkage disequilibrium between rs1800795 (-174G C) and rs1800797 (-597G A) showed that individuals having AC* haplotype significantly association with COVID-19 severity (p = .034). Our results suggest that 'C' allele of rs1800795 (-174G C) polymorphism of IL6 may be the risk allele for severity of COVID-19 in North Indian population.