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Your search keyword '"Haiqa Saif"' showing total 2 results
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2 results on '"Haiqa Saif"'

1. List of contributors

Author

Sadanandam Abbagani, Abdul Zahir Abbasi, Kamel A. Abd-Elsalam, Muhammad Amin Afzal, Shakeel Ahmad, Farah K. Ahmed, Muhammad Junaid Akhtar, Tasmina Akter, Mousa Alghuthaymi, Badr Alharthi, Habib Ali, Naushad Ali, Venkateswar Rao Allini, Anshu Alok, Vinoth Alphonse, Raúl Alvarez-Venegas, Gacem Mohamed Amine, Galal Bakr Anis, Muhammad Ashraf, Umair Ashraf, Rana Muhammad Atif, Muhammad Awais, Uzma Badar, Aftab Bashir, Ashita Bisht, Djoudi Boukerouis, Rajkuberan Chandrasekaran, Muddassir Ayaz Mahmood Chaudhary, Jeyapragash Danaraj, Alisha Doda, Sayan Deb Dutta, Rimsha Farooq, Sajid Fiaz, Mahmoud Mohamed Gaballah, Hiba Gacem, Mohamed Amine Gacem, Vijay Gahlaut, Prabuddha Ganguli, Keya Ganguly, Vikrant Gautam, Saira Ghafoor, Siddhesh B. Ghag, Arun Gokul, Shanmugam Gowsalya, Amir Hameed, Kathleen Hefferon, Peisong Hu, Shikai Hu, Khadim Hussain, Md Salman Hyder, Anam Ijaz, Muhammad Munir Iqbal, Tofazzal Islam, Prateek Jain, Vandana Jaiswal, Rewaa S. Jalal, Shakra Jamil, Muhammad Rizwan Javed, Phanikanth Jogam, Luo Ju, Kattesh V. Katti, Marshall Keyster, Sher Aslam Khan, Ashwil Klein, Pankaj Kumar, Sanjay Kumar, Ki-Taek Lim, Alberto Cristian López-Calleja, Yusong Lv, Zahid Majeed, Victoria Maloney, Johnson Marimuthu alias Antonysamy, Priyank H. Mhatre, Mahmuda Binte Monsur, Manal Mostafa, Kasi Murugan, Faisal Nadeem, Habibullah Nadeem, Mehmood Ali Noor, Valentine Otang Ntui, Aminata Ould-El-Hadj-Khelil, Sanjoy Kumar Paul, Kanniah Paulkumar, Dinesh Prasad, Abdul Qayyum, Murugan Raghul, P. Rajiv, Kamran Rashid, Abdur Raziq, Aamir Riaz, Haiqa Saif, Dulam Sandhya, Satyajit Saurabh, Prabu Kumar Seetharaman, Muhammad Shahid, Khurram Shahzad, Rahil Shahzad, Ashwag Shami, Gaoneng Shao, Zhonghua Sheng, Erum Shoeb, Zeeshan Shokat, Arul raj Suriya Jasmin, Javaria Tabassum, Shaoqing Tang, Alia Telli, Velaphi C. Thipe, Jaindra Nath Tripathi, Leena Tripathi, Basit Umer, Srividhya Venkataraman, Juan Carlos Vizuet-de-Rueda, Santosh G. Watpade, Xiangjin Wei, Joachim Wink, Poomany Arul Soundara Rajan Yolin Angel, and Afifa Younas

Published
2021
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2. Tricks and trends in CRISPR/Cas9-based genome editing and use of bioinformatics tools for improving on-target efficiency

Author

Kamran Rashid, Aftab Bashir, Muhammad Rizwan Javed, Haiqa Saif, Rimsha Farooq, and Khadim Hussain

Subjects
Genome editing, biology, Computer science, Cas9, biology.protein, CRISPR, Mutagenesis (molecular biology technique), Transcription (software), Bioinformatics, Genome, Zinc finger nuclease, Homing endonuclease
Abstract

Precision in genome editing has a long history of adaptation from zinc finger nucleases to transcription activator-like effector nucleases, LAGLIDADG homing endonucleases, oligonucleotide-directed mutagenesis, and finally CRISPR/Cas technology. The bacterial-derived adaptive immune system has been exploited to artificially mimic the CRISPR/Cas9-based genome editing into other eukaryotic organisms. This robust and simple technology requires only two key components (sgRNA and Cas9) for precise editing, but this versatile tool has been struggling with an issue of off-site targeting. Initially, seed region of SgRNA has been a focal point to improve specificity, and several rules to develop efficient gRNAs were identified. Those rules were valid for simpler organisms but major specificity problems were concerned with complex organisms, that is, plants with higher ploidy levels. Different algorithms and machine learning models are based on different experimentally validated datasets that predict best if CRISPR-experimental conditions of querier data also matched with initial datasets used in building those models. The good bioinformatic tools are designed on a large amount of CRISPR-edited NGS data and support multiple genomes to predict an efficient sgRNA design by maximizing on-target and minimizing off-target scores. This paper describes both sgRNA designing rules and the available bioinformatics tools for efficient sgRNA design.

Published
2021
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