246 results on '"Haiyan Qi"'
Search Results
2. Development of a simple and miniaturized sandwich-like fluorescence polarization assay for rapid screening of SARS-CoV-2 main protease inhibitors
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Gangan Yan, Dongsheng Li, Yuan Lin, Zhenghao Fu, Haiyan Qi, Xiaoping Liu, Jing Zhang, Shuyi Si, and Yunyu Chen
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SARS-CoV-2 ,Main protease inhibitor ,3CL protease inhibitor ,Fluorescence polarization ,High-throughput screening ,Dieckol ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly transmissible and has caused a pandemic named coronavirus disease 2019 (COVID-19), which has quickly spread worldwide. Although several therapeutic agents have been evaluated or approved for the treatment of COVID-19 patients, efficacious antiviral agents are still lacking. An attractive therapeutic target for SARS-CoV-2 is the main protease (Mpro), as this highly conserved enzyme plays a key role in viral polyprotein processing and genomic RNA replication. Therefore, the identification of efficacious antiviral agents against SARS-CoV-2 Mpro using a rapid, miniaturized and economical high-throughput screening (HTS) assay is of the highest importance at the present. Results In this study, we first combined the fluorescence polarization (FP) technique with biotin-avidin system (BAS) to develop a novel and step-by-step sandwich-like FP screening assay to quickly identify SARS-CoV-2 Mpro inhibitors from a natural product library. Using this screening assay, dieckol, a natural phlorotannin component extracted from a Chinese traditional medicine Ecklonia cava, was identified as a novel competitive inhibitor against SARS-CoV-2 Mpro in vitro with an IC50 value of 4.5 ± 0.4 µM. Additionally, dieckol exhibited a high affinity with SARS-CoV-2 Mpro using surface plasmon resonance (SPR) analysis and could bind to the catalytic sites of Mpro through hydrogen-bond interactions in the predicted docking model. Conclusions This innovative sandwich-like FP screening assay enables the rapid discovery of antiviral agents targeting viral proteases, and dieckol will be an excellent lead compound for generating more potent and selective antiviral agents targeting SARS-CoV-2 Mpro.
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- 2021
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3. Using Apatite to Track Volatile Evolution in the Shallow Magma Chamber below the Yonaguni Knoll IV Hydrothermal Field in the Southwestern Okinawa Trough
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Zuxing Chen, Landry Soh Tamehe, Haiyan Qi, Yuxiang Zhang, Zhigang Zeng, and Mingjiang Cai
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apatite ,volatiles ,thermodynamic modeling ,Yonaguni Knoll IV hydrothermal field ,Okinawa Trough ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
The Yonaguni Knoll IV is an active seafloor hydrothermal system associated with submarine silicic volcanism located in the “cross back-arc volcanic trail” (CBVT) in the southwestern Okinawa Trough. However, the behavior of volatiles during magmatic differentiation in the shallow silicic magma chamber is unclear. Here, the volatile contents of apatite inclusions trapped in different phenocrysts (orthopyroxene and amphibole) and microphenocrysts in the rhyolite from the Yonaguni Knoll IV hydrothermal field were analyzed by using electron microprobe analysis, which aims to track the behavior of volatiles in the shallow magma chamber. Notably, the ‘texturally constrained’ apatites showed a decreasing trend of XCl/XOH and XF/XCl ratios. Based on the geochemical analyses in combination with thermodynamic modeling, we found that the studied apatites were consistent with the mode of volatile-undersaturated crystallization. Therefore, volatiles were not saturated in the early stage of magmatic differentiation in the shallow rhyolitic magma chamber, and consequently, the metal elements were retained in the rhyolitic melt and partitioned into crystalline magmatic sulfides. Additionally, previous studies suggested that the shallow rhyolitic magma chamber was long-lived and periodically replenished by mafic magma. The injection of volatile-rich and oxidized subduction-related mafic magmas can supply abundant volatiles and dissolve magmatic sulfide in the shallow magma chamber. These processes are important for the later-stage of volatile exsolution, while the forming metal-rich magmatic fluids contribute to the overlying Yonaguni Knoll IV hydrothermal system.
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- 2023
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4. Strontium, Hydrogen and Oxygen Behavior in Vent Fluids and Plumes from the Kueishantao Hydrothermal Field Offshore Northeast Taiwan: Constrained by Fluid Processes
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Zhigang Zeng, Xiaoyuan Wang, Xuebo Yin, Shuai Chen, Haiyan Qi, and Chen-Tung Arthur Chen
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strontium-hydrogen-oxygen isotopes ,vent fluid ,hydrothermal plume ,Kueishantao hydrothermal field ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
Strontium (Sr), hydrogen (H) and oxygen (O) in vent fluids are important for understanding the water–rock interaction and hydrothermal flux in hydrothermal systems. We have analyzed the Sr, H and O isotopic compositions of seawater, vent fluid and hydrothermal plume samples in the Kueishantao hydrothermal field, as well as their calcium (Ca), total sulfur (S), Sr, arsenic (As), stibium (Sb), chlorine (Cl) and manganese (Mn) concentrations for understanding the origin and processes of fluids. The results suggest that most As, Sb and Mn are leached from andesitic rocks into the fluids, and most Ca and Cl remained in the deep reaction zone during the fluid–andesitic rock interaction. The ranges of 87Sr/86Sr, δDV-SMOW and δ18OV-SMOW values in the yellow spring, white spring and plumes are small. The 87Sr/86Sr, δDV-SMOW and δ18OV-SMOW values of fluids and plumes are like those of ambient seawater, indicating that the Sr, H and O of vent fluids and hydrothermal plumes are derived primarily from seawater. This suggests that the interaction of andesite and subseafloor fluid is of short duration and results in the majority of As, Sb and Mn being released into fluids, while most Ca and Cl remained in the deep reaction zone. In addition, there was no significant variation of Sr, H and O isotopic compositions in the upwelling fluid, keeping the similar isotopic compositions of seawater. There are obvious correlations among the pH values, As and Sb concentrations, and H isotopic compositions of the vent fluids and hydrothermal plumes, implying that the As and Sb concentrations and H isotopic compositions can trace the dispersion of plumes in the ambient seawater. According to the Sr concentrations and 87Sr/86Sr values, the water/rock ratios are 3076~8124, which is consistent with the idea that the interaction between fluid and andesite at the subseafloor is of short duration. The hydrothermal flux of Sr discharged from the yellow spring into the seawater is between 2.06 × 104 and 2.26 × 104 mol/yr, and the white spring discharges 1.18 × 104~1.26 × 104 mol/yr Sr if just andesites appear in the reaction zone.
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- 2022
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5. Chemical and Isotopic Composition of Sulfide Minerals from the Noho Hydrothermal Field in the Okinawa Trough
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Zhigang Zeng, Zuxing Chen, Haiyan Qi, and Bowen Zhu
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element enrichment ,hydrothermal vent ,in-situ chemical and isotopic compositions ,seafloor hydrothermal sulfides ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
Studies of the element contents and isotopic characteristics of sulfide minerals from seafloor hydrothermal sulfide deposits are a significant method of investigating seawater-fluid mixing and fluid-rock and/or sediment interactions in hydrothermal systems. The seafloor hydrothermal sulfide ores from the Noho hydrothermal field (NHF) in the Okinawa Trough (OT) consist of pyrrhotite, isocubanite, sphalerite, galena, and amorphous silica. The Rh, Ag, Sb, and Tl contents mostly increase in galena as the fluid temperature decreases in the late ore-forming stage. In the sulfide minerals, the rare earth elements are mainly derived from the hydrothermal fluids, while the volcanic rocks and/or sediments are the sources of the sulfur and lead in the sulfide minerals. After the precipitation of galena, the redox state becomes oxidizing, and the pH value of the fluid increases, which is accompanied by the formation of amorphous silica. Finally, neither pyrite nor marcasite has been observed in association with pyrrhotite in the NHF sulfides, likely indicating that the amount of sulfur was limited in this hydrothermal system, and most of the residual Fe was incorporated into the sphalerite. This suggests that the later pyrite and/or marcasite precipitation in the seafloor hydrothermal sulfide deposit is controlled by the sulfur content of the fluid. Furthermore, it is possible to use hydrothermal sulfides and their inclusions to trace subseafloor fluid circulation processes.
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- 2022
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6. Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen
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Haiyan Qi, Qiuying Li, Jing Jing, Tao Jing, Chuntong Liu, Lixin Qiu, Rokayya Sami, Mahmoud Helal, Khadiga Ahmed Ismail, and Amani H. Aljahani
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N-CDs ,calcein ,ratiometric fluorescent sensor ,arginine ,acetaminophen ,Chemistry ,QD1-999 - Abstract
In our study, a unique ratiometric fluorescent sensor for the rapid detection of arginine (Arg) and acetaminophen (AP) was constructed by the integration of blue fluorescent N-CDs and yellowish-green fluorescent calcein. The N-CD/calcein ratiometric fluorescent sensor exhibited dual emission at 435 and 519 nm under the same excitation wavelength of 370 nm, and caused potential Förster resonance energy transfer (FRET) from N-CDs to calcein. When detecting Arg, the blue fluorescence from the N-CDs of the N-CD/calcein sensor was quenched by the interaction of N-CDs and Arg. Then, the fluorescence of our sensor was recovered with the addition of AP, possibly due to the stronger association between AP and Arg, leading to the dissociation of Arg from N-CDs. Meanwhile, we observed an obvious fluorescence change from blue to green, then back to blue, when Arg and AP were added, exhibiting the “on–off–on” pattern. Next, we determined the detection limits of the N-CD/calcein sensor to Arg and AP, which were as low as 0.08 μM and 0.02 μM, respectively. Furthermore, we discovered that the fluorescence changes of the N-CD/calcein sensor were only responsible for Arg and AP. These results suggested its high sensitivity and specificity for Arg and AP detection. In addition, we have successfully achieved its application in bovine serum samples, indicating its practicality. Lastly, the logic gate was generated by the N-CD/calcein sensor and presented its good reversibility. Overall, we have demonstrated that our N-CD/calcein sensor is a powerful sensor to detect Arg and AP and that it has potential applications in biological analysis and imaging.
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- 2022
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7. Identification of MicroRNA-92a-3p as an Essential Regulator of Tubular Epithelial Cell Pyroptosis by Targeting Nrf1 via HO-1
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Renhe Wang, Haijun Zhao, Yingyu Zhang, Hai Zhu, Qiuju Su, Haiyan Qi, Jun Deng, and Chengcheng Xiao
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renal ischemia–reperfusion injury ,miR-92a-3p ,Nrf1 ,pyroptosis ,tubular epithelial cell ,HO-1 ,Genetics ,QH426-470 - Abstract
Renal ischemia–reperfusion injury (IRI) is a major cause of acute kidney injury (AKI) and has no effective treatment. Exploring the molecular mechanisms of renal IRI is critical for the prevention of AKI and its evolution to chronic kidney disease and end-stage renal disease. The aim of the present study was to determine the biological function and molecular mechanism of action of miR-92a-3p in tubular epithelial cell (TEC) pyroptosis. We investigated the relationship between nuclear factor-erythroid 2-related factor 1 (Nrf1) and TEC pyroptosis induced by ischemia–reperfusion in vivo and oxygen–glucose deprivation/reoxygenation (OGD/R) in vitro. MicroRNAs (miRNAs) are regulators of gene expression and play a role in the progression of renal IRI. Nrf1 was confirmed as a potential target for miRNA miR-92a-3p. In addition, the inhibition of miR-92a-3p alleviated oxidative stress in vitro and decreased the expression levels of NLRP3, caspase-1, GSDMD-N, IL-1β, and IL-18 in vitro and in vivo. Moreover, Zn-protoporphyrin-IX, an inhibitor of heme oxygenase-1, reduced the protective effect of Nrf1 overexpression on OGD/R-induced TEC oxidative stress and pyroptosis. The results of this study suggest that the inhibition of miR-92a-3p can alleviate TEC oxidative stress and pyroptosis by targeting Nrf1 in renal IRI.
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- 2021
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8. Identification of a Seven-lncRNA-mRNA Signature for Recurrence and Prognostic Prediction in Relapsed Acute Lymphoblastic Leukemia Based on WGCNA and LASSO Analyses
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Haiyan Qi, Long Chi, Xiaogang Wang, Xing Jin, Wensong Wang, and Jianping Lan
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abnormal expressions of long noncoding RNAs (lncRNAs) and protein-encoding messenger RNAs (mRNAs) are important for the development of childhood acute lymphoblastic leukemia (ALL). This study developed an lncRNA-mRNA integrated classifier for the prediction of recurrence and prognosis in relapsed childhood ALL by using several transcriptome data. Weighted gene coexpression network analysis revealed that green, turquoise, yellow, and brown modules were preserved across the TARGET, GSE60926, GSE28460, and GSE17703 datasets, and they were associated with clinical relapse and death status. A total of 184 genes in these four modules were differentially expressed between recurrence and nonrecurrence samples. Least absolute shrinkage and selection operator analysis showed that seven genes constructed a prognostic signature (including one lncRNA: LINC00652 and six mRNAs: INSL3, NIPAL2, REN, RIMS2, RPRM, and SNAP91). Kaplan-Meier curve analysis observed that patients in the high-risk group had a significantly shorter overall survival than those of the low-risk group. Receiver operating characteristic curve analysis demonstrated that this signature had high accuracy in predicting the 5-year overall survival and recurrence outcomes, respectively. LINC00652 may function by coexpressing with the above prognostic genes (INSL3, SNAP91, and REN) and lipid metabolism-related genes (MIA2, APOA1). Accordingly, this lncRNA-mRNA-based classifier may be clinically useful to predict the recurrence and prognosis for childhood ALL. These genes represent new targets to explain the mechanisms for ALL.
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- 2021
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9. A Continuously Tunable Full-Color Emission Nitrogen-Doped Carbon Dots and for Ultrasensitive and Highly Selective Detection of Ascorbic Acid
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Demin Huang, Haiyan Qi, Jing Jing, Rokayya Sami, Tao Jing, Sultan J. Alsufyani, Nada Benajiba, and Nawal Madkhali
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nitrogen-doped carbon dots ,full-color emission ,ascorbic acid ,fluorescence detection ,Chemistry ,QD1-999 - Abstract
Nitrogen-doped carbon dots exhibiting excitation-dependent full-color emissions (F-NCDs) were prepared via the one-step hydrothermal method with citric acid and phenylenediamine. Specifically, the emission wavelength of the F-NCDs tuned from 452 nm to 602 nm due to the introduction of new energy levels by C=O and C=N functional groups. We exploited its stability in illumination, ionic strength, and pH, as well as its specificity, sensitivity, especially in ascorbic acid (AA) detection. F-NCDs could measure the AA concentration in the linear ranges of 0~0.1 and 0.1~1 mmol/L with the detection limit (LOD, S/N = 3) as low as 2.6 nmol/L. Additionally, we successfully detected AA in bovine serum with our F-NCDs and obtained the result within 1 min. Because of full-color emission features, we believe our F-NCDs have a great potential in fluorescent sensor detection.
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- 2022
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10. Two Processes of Anglesite Formation and a Model of Secondary Supergene Enrichment of Bi and Ag in Seafloor Hydrothermal Sulfide Deposits
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Zhigang Zeng, Zuxing Chen, and Haiyan Qi
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anglesite ,element distribution and mobility ,supergene Ag–Bi enrichment ,sulfide ,hydrothermal vent ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
The in situ element concentrations and the sulfur (S), and lead (Pb) isotopic compositions in anglesite were investigated for samples from seafloor hydrothermal fields in the Okinawa Trough (OT), Western Pacific. The anglesite grains are of two kinds: (1) low Pb/high S primary hydrothermal anglesite (PHA), which is formed by mixing of fluid and seawater, and (2) high Pb/low S secondary supergene anglesite (SSA), which is the product of low-temperature (
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- 2021
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11. Lithium, Oxygen and Magnesium Isotope Systematics of Volcanic Rocks in the Okinawa Trough: Implications for Plate Subduction Studies
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Zhigang Zeng, Xiaohui Li, Yuxiang Zhang, and Haiyan Qi
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Li-O-Mg isotopes ,magma ,plate subduction ,Okinawa Trough ,western Pacific ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
Determining the influence of subduction input on back-arc basin magmatism is important for understanding material transfer and circulation in subduction zones. Although the mantle source of Okinawa Trough (OT) magmas is widely accepted to be modified by subducted components, the role of slab-derived fluids is poorly defined. Here, major element, trace element, and Li, O and Mg isotopic compositions of volcanic lavas from the middle OT (MOT) and southern OT (SOT) were analyzed. Compared with the MOT volcanic lavas, the T9-1 basaltic andesite from the SOT exhibited positive Pb anomalies, significantly lower Nd/Pb and Ce/Pb ratios, and higher Ba/La ratios, indicating that subducted sedimentary components affected SOT magma compositions. The δ7Li, δ18O, and δ26Mg values of the SOT basaltic andesite (−5.05‰ to 4.98‰, 4.83‰ to 5.80‰ and −0.16‰ to −0.09‰, respectively) differed from those of MOT volcanic lavas. Hence, the effect of the Philippine Sea Plate subduction component, (low δ7Li and δ18O and high δ26Mg) on magmas in the SOT was clearer than that in the MOT. This contrast likely appears because the amounts of fluids and/or melts derived from altered oceanic crust (AOC, lower δ18O) and/or subducted sediment (lower δ7Li, higher δ18O and δ26Mg) injected into magmas in the SOT are larger than those in the MOT and because the injection ratio between subducted AOC and sediment is always >1 in the OT. The distance between the subducting slab and overlying magma may play a significant role in controlling the differences in subduction components injected into magmas between the MOT and SOT.
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- 2021
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12. Dispersion and Intersection of Hydrothermal Plumes in the Manus Back-Arc Basin, Western Pacific
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Zhigang Zeng, Xiaoyuan Wang, Bramley J. Murton, Haiyan Qi, Berit Lehrmann, Xiaohui Li, Zuxing Chen, and Yunchao Shu
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Geology ,QE1-996.5 - Abstract
The composition of hydrothermal plumes reflects the physical and chemical characteristics of seafloor hydrothermal fluids, which in turn reflects the host rock and subseafloor reaction conditions as well as the water column processes that act to alter the plumes as they disperse and age. Here, we show that the turbidity, current, pH value, dissolved Fe (dFe), and dissolved Mn (dMn) compositions of hydrothermal plumes can be used to understand the spatial distribution and source of hydrothermal systems in the submarine geological environment. Data were obtained from 18 hydrocast stations, among which the water column samples were collected at 8 stations during the MANUS cruise of R/V KEXUE in 2015. The results showed that the Satanic Mills plume and Fenway plume rose approximately 140 m and 220 m above the seafloor, respectively. In the Satanic Mills plume, dFe remained longer than dMn during lateral plume dispersal. There was a clear intersection of the Satanic Mills plume and Fenway plume between 1625 m and 1550 m in the PACMANUS hydrothermal field, and the varied dispersion trends of the mixed plumes were affected by current velocities at different depths. The physical and chemical properties of the seawater columns in the Manus Basin were affected by the input of high-Mn, high-Fe, and low-Mg vent fluids. The turbidity and dFe, dMn, and dissolved Mg concentrations in the sections of the plumes proximal to the Satanic Mills, Fenway, and Desmos vent sites were generally higher (turbidity, Mn, and Fe) and lower (Mg) than those in the sections of the plumes that were more distal from the vent sites. This implied that the plumes proximal to their vent fluid sources, which were interpreted to have relatively young ages, dispersed chemically over time, and their concentrations became more similar to those of the plumes that were more distal from their vent fluid sources.
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- 2020
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13. Preparation of Eu0.075Tb0.925-Metal Organic Framework as a Fluorescent Probe and Application in the Detection of Fe3+ and Cr2O72−
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Jie Yin, Hongtao Chu, Shili Qin, Haiyan Qi, and Minggang Hu
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Ln-MOFs ,luminous sensing ,Fe3+ ,Cr2O72− ,Chemical technology ,TP1-1185 - Abstract
Luminescent Ln-MOFs (Eu0.075Tb0.925-MOF) were successfully synthesised through the solvothermal reaction of Tb(NO3)3·6H2O, Eu(NO3)3·6H2O, and the ligand pyromellitic acid. The product was characterised by X-ray diffraction (XRD), TG analysis, EM, X-ray photoelectron spectroscopy (XPS), and luminescence properties, and results show that the synthesised material Eu0.075Tb0.925-MOF has a selective ratio-based fluorescence response to Fe3+ or Cr2O72−. On the basis of the internal filtering effect, the fluorescence detection experiment shows that as the concentration of Fe3+ or Cr2O72− increases, the intensity of the characteristic emission peak at 544 nm of Tb3+ decreases, and the intensity of the characteristic emission peak at 653 nm of Eu3+ increases in Eu0.075Tb0.925-MOF. The fluorescence intensity ratio (I653/I544) has a good linear relationship with the target concentration. The detection linear range for Fe3+ or Cr2O72− is 10–100 μM/L, and the detection limits are 2.71 × 10−7 and 8.72 × 10−7 M, respectively. Compared with the sensor material with a single fluorescence emission, the synthesised material has a higher anti-interference ability. The synthesised Eu0.075Tb0.925-MOF can be used as a highly selective and recyclable sensing material for Fe3+ or Cr2O72−. This material should be an excellent candidate for multifunctional sensors.
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- 2021
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14. Geochemical Study of Detrital Apatite in Sediment from the Southern Okinawa Trough: New Insights into Sediment Provenance
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Siyi Hu, Zhigang Zeng, Xue Fang, Haiyan Qi, Xuebo Yin, Zuxing Chen, Xiaohui Li, and Bowen Zhu
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detrital apatite ,sediment provenance ,geochemical composition ,the southern okinawa trough ,Mineralogy ,QE351-399.2 - Abstract
Detrital apatite is a promising accessory mineral for reliable fingerprinting of the protolith composition of detrital sediment. Here, we present the geochemical compositions of detrital apatites from four sections of a high-resolution (~140 cm/kyr) sedimentary archive from the southern Okinawa Trough (SOT) for constraining sediment source rock types and establishing changes in provenance. A series of diagrams based on the geochemical data show that apatites in Section 1 derived mainly from mafic/intermediate rocks, while apatites in the three younger sections largely originated from mafic/intermediate rocks and acid rocks. Further, the four sections included a few contributions of apatites from alkaline rocks and metamorphic rocks. Through a comprehensive analysis of controlling factors and regional stratigraphy in the study area, we concluded that sediments in the SOT are the weathering/erosion products of sedimentary and meta-sedimentary rocks in the western and northeastern Taiwan Island, with ultimate sources containing a wide variety of rock types. The sediment from rivers in eastern Taiwan did not significantly contribute to deposition in the SOT. Apatites in Sections 2−4 showed strong geochemical similarity but differed from those in Section 1, indicating that the types of weathered/eroded sedimentary rocks in the weathering profile of river drainage basins of Taiwan Island changed in Section 2 (2010−1940 cal. yr BP) and have remained stable since then.
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- 2019
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15. Detection of ethyl carbamate in liquors using surface-enhanced Raman spectroscopy
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Haiyan Qi, Huacai Chen, Yan Wang, and Li Jiang
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surface-enhanced raman scattering (sers) ,ethyl carbamate ,flower-shaped silver nanostructure substrate ,silver nanocubes ,linear regression model ,Science - Abstract
Ethyl carbamate (EC), a potential carcinogen, can be formed during the fermentation and storage of alcoholic beverages. In this work, quantitative detection of EC in alcoholic beverages by using surface-enhanced Raman spectroscopy (SERS) is reported. Flower-shaped silver nanostructure substrates and silver nanocube substrates were prepared and employed as SERS platform. Flower-like silver substrates had better Raman enhancement effect on EC and were selected for further EC detection. In EC SERS spectra based on flower-shaped silver substrates, the strongest and reproducible characteristic band at 857 cm−1 was chosen for establishing a linear regression model in the concentrations ranging from 10−5 to 10−9 M, which effectively extended the application scope of the quantitative model for determination EC. Furthermore, a real alcoholic beverage was tested to verify the feasibility and reliability of the method.
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- 2018
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16. Understanding the Compositional Variability of the Major Components of Hydrothermal Plumes in the Okinawa Trough
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Zhigang Zeng, Xiaoyuan Wang, Chen-Tung Arthur Chen, and Haiyan Qi
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Geology ,QE1-996.5 - Abstract
Studies of the major components of hydrothermal plumes in seafloor hydrothermal fields are critical for an improved understanding of biogeochemical cycles and the large-scale distribution of elements in the submarine environment. The composition of major components in hydrothermal plume water column samples from 25 stations has been investigated in the middle and southern Okinawa Trough. The physical and chemical properties of hydrothermal plume water in the Okinawa Trough have been affected by input of the Kuroshio current, and its influence on hydrothermal plume water from the southern Okinawa Trough to the middle Okinawa Trough is reduced. The anomalous layers of seawater in the hydrothermal plume water columns have higher K+, Ca2+, Mn2+, B3+, Ca2+/SO42-, and Mn2+/Mg2+ ratios and higher optical anomalies than other layers. The Mg2+, SO42-, Mg2+/Ca2+, and SO42-/Mn2+ ratios of the anomalous layers are lower than other layers in the hydrothermal plume water columns and are consistent with concentrations in hydrothermal vent fluids in the Okinawa Trough. This suggests that the chemical variations of hydrothermal plumes in the Tangyin hydrothermal field, like other hydrothermal fields, result in the discharge of high K+, Ca2+, and B3+ and low Mg2+ and SO42- fluid. Furthermore, element ratios (e.g., Sr2+/Ca2+, Ca2+/Cl−) in hydrothermal plume water columns were found to be similar to those in average seawater, indicating that Sr2+/Ca2+ and Ca2+/Cl− ratios of hydrothermal plumes might be useful proxies for chemical properties of seawater. The hydrothermal K+, Ca2+, Mn2+, and B3+ flux to seawater in the Okinawa Trough is about 2.62–873, 1.04–326, 1.30–76.4, and 0.293–34.7 × 106 kg per year, respectively. The heat flux is about 0.159–1,973 × 105 W, which means that roughly 0.0006% of ocean heat is supplied by seafloor hydrothermal plumes in the Okinawa Trough.
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- 2018
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17. Arsenic and Antimony in Hydrothermal Plumes from the Eastern Manus Basin, Papua New Guinea
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Zhigang Zeng, Xiaoyuan Wang, Haiyan Qi, and Bowen Zhu
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Geology ,QE1-996.5 - Abstract
Studies on the concentrations of arsenic (As) and antimony (Sb) in seawater columns are very important for tracing hydrothermal plumes and understanding fluid characteristics of seafloor hydrothermal systems. The total As, Sb, Mn, and Cl− concentrations of three hydrothermal plume seawater column samples have been studied at Stations 18G, 18K, and 18B in the eastern Manus back-arc basin, Bismarck Sea, Papua New Guinea. At Stations 18G and 18K, the plumes above North Su and near the Suzette site in the SuSu Knolls hydrothermal field are both enriched in As, Sb, and Mn and depleted in Cl, as a result of contribution of As-Sb-Mn-enriched and Cl-depleted vent fluid outputs to the hydrothermal plume, which is most likely generated in the subseafloor by fluid-rock interaction, magma degassing, or phase separation (boiling of hydrothermal fluid). The plume at Station 18B is enriched in As, Sb, Mn, and Cl, suggesting that As-Sb-Mn-Cl-enriched fluid discharges from vents, which have been generated by fluid-rock interaction. The concentrations of As and Sb anomalous layers, like manganese (Mn), are higher than those of the other layers in the three hydrothermal plume seawater columns. As and Sb with Mn showed a positive correlation (R2>0.8, p
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- 2018
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18. A Meta Analysis of Doublets Versus Single-agent Chemotherapy for Elderly Patients with Advanced Non-small Cell Lung Cancer
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Chong’an XU, Ziyou CHANG, Xiaojie WANG, Lin LI, Haiyan QI, and Yan LIU
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Lung neoplasms ,Aged ,Chemotherapy ,Meta-analysis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background and objective It remains disputed whether doublets are more effective than single-agent chemotherapy for elderly patients with advanced non-small cell lung cancer (NSCLC). The aim of this study is to evaluate the efficacy and safety of doublets and single-agent chemotherapy for elderly patients with NSCLC. Methods Data from all published, randomized trials that compared doublets and single-agent chemotherapy in elderly patients were collected from electronic databases (PubMed, EMBASE, Cochrane Library, CNKI and the CBMdice). Meta-analysis was completed using software Stata 11.0. Results The results of the meta-analysis, including 12 eligible trials (2,306 patients), showed that the doublets significantly increased the overall response rate (OR=1.80, 95%CI:1.50-2.17, P
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- 2012
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19. Survivin -31G>C polymorphism and gastrointestinal tract cancer risk: a meta-analysis.
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Yan Liu, Lin Li, Haiyan Qi, Yan Gao, Sha Liu, and Chongan Xu
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Medicine ,Science - Abstract
BACKGROUND: Emerging evidence showed that common functional -31G>C polymorphism (rs9904341 G>C) in the promoter region of the survivin gene is involved in the regulation of survivin expression, thus increasing an individual's susceptibility to gastrointestinal tract (GIT) cancer; but individually published results are inconclusive. The aim of this systematic review and meta-analysis was to derive a more precise estimation of the association between survivin -31G>C polymorphism and GIT cancer risk. METHODS: A literature search of PubMed, Embase, Web of Science and CBM databases was conducted from inception through July 1st, 2012. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of association. RESULTS: Nine case-control studies were included with a total of 2,231 GIT cancer cases and 2,287 healthy controls. The results indicated that survivin -31G>C polymorphism was associated with increased risk of GIT cancer. In the stratified analysis by cancer types, significant associations were observed between survivin -31G>C polymorphism and increased risk of colorectal and gastric cancers. However, the lack of association of survivin -31G>C polymorphism with esophageal cancer risk may be due to a lack of a sufficient number of eligible studies and the influence of different genetic and environmental factors. CONCLUSION: Results from the current meta-analysis suggests that survivin -31G>C polymorphism might increase the risk of GIT cancer, especially among gastric and colorectal cancers.
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- 2013
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20. HIV-1 diversity, drug-resistant mutations, and viral evolution among high-risk individuals in phase II HIV vaccine trial sites in southern China.
- Author
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Haiyan Qi, Ke Zhao, Fei Xu, Xuzhao Zhang, Zhiyong Zhang, Li Yang, Chunling Li, Xu Liang, Weigui Guo, Shihai Chen, Zhihao Liu, Wenyan Zhang, and Xiao-Fang Yu
- Subjects
Medicine ,Science - Abstract
HIV-1 prevalence in Guangxi, China, has been growing since 1996, when the first case was reported. Over half of HIV-1 positive patients in Guangxi Province were injecting drug users (IDUs), possibly because of the province's location near drug-trafficking routes. Since a phase II HIV vaccine trial is ongoing there, a current characterization of the subtypes of HIV-1 among IDUs in Guangxi would provide critical information for future HIV vaccine trials, as well as further control and prevention of HIV-1 transmission. Thus, we conducted a molecular epidemiological investigation of HIV-1 samples from 2008-2010 among IDUs in multiple cities in Guangxi Province. Our results, based on the gag/pol fragment, indicated a very high proportion (78.47%) of HIV-1 CRF08_BC recombinants, some CRF01_AE (15.38%) recombinants, and a low proportion of CRF07_BC (6.15%) recombinants among the IDUs. The high proportion of CRF08 HIV-1 strains among recent IDUs matches the vaccine candidate constructs. However, future vaccine development should also incorporate CRF01-targeted vaccine candidates. Distinct Env sequence evolution patterns were observed for CRF08_BC and CRF01_AE, indicating that different local selection pressures have been exerted on these two HIV-1 subtypes. Unique drug-resistant mutations were also detected, and our data indicate that HIV treatment programs should consider pre-existing drug-resistant mutations.
- Published
- 2013
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21. Etoposide induces ATM-dependent mitochondrial biogenesis through AMPK activation.
- Author
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Xuan Fu, Shan Wan, Yi Lisa Lyu, Leroy F Liu, and Haiyan Qi
- Subjects
Medicine ,Science - Abstract
DNA damage such as double-stranded DNA breaks (DSBs) has been reported to stimulate mitochondrial biogenesis. However, the underlying mechanism is poorly understood. The major player in response to DSBs is ATM (ataxia telangiectasia mutated). Upon sensing DSBs, ATM is activated through autophosphorylation and phosphorylates a number of substrates for DNA repair, cell cycle regulation and apoptosis. ATM has been reported to phosphorylate the alpha subunit of AMP-activated protein kinase (AMPK), which senses AMP/ATP ratio in cells, and can be activated by upstream kinases. Here we provide evidence for a novel role of ATM in mitochondrial biogenesis through AMPK activation in response to etoposide-induced DNA damage.Three pairs of human ATM+ and ATM- cells were employed. Cells treated with etoposide exhibited an ATM-dependent increase in mitochondrial mass as measured by 10-N-Nonyl-Acridine Orange and MitoTracker Green FM staining, as well as an increase in mitochondrial DNA content. In addition, the expression of several known mitochondrial biogenesis regulators such as the major mitochondrial transcription factor NRF-1, PGC-1alpha and TFAM was also elevated in response to etoposide treatment as monitored by RT-PCR. Three pieces of evidence suggest that etoposide-induced mitochondrial biogenesis is due to ATM-dependent activation of AMPK. First, etoposide induced ATM-dependent phosphorylation of AMPK alpha subunit at Thr172, indicative of AMPK activation. Second, inhibition of AMPK blocked etoposide-induced mitochondrial biogenesis. Third, activation of AMPK by AICAR (an AMP analogue) stimulated mitochondrial biogenesis in an ATM-dependent manner, suggesting that ATM may be an upstream kinase of AMPK in the mitochondrial biogenesis pathway.These results suggest that activation of ATM by etoposide can lead to mitochondrial biogenesis through AMPK activation. We propose that ATM-dependent mitochondrial biogenesis may play a role in DNA damage response and ROS regulation, and that defect in ATM-dependent mitochondrial biogenesis could contribute to the manifestations of A-T disease.
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- 2008
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22. TOR regulates cell death induced by telomere dysfunction in budding yeast.
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Haiyan Qi, Yongjie Chen, Xuan Fu, Chao-Po Lin, X F Steven Zheng, and Leroy F Liu
- Subjects
Medicine ,Science - Abstract
Telomere dysfunction is known to induce growth arrest (senescence) and cell death. However, the regulation of the senescence-death process is poorly understood. Here using a yeast dysfunctional telomere model cdc13-1, which carries a temperature sensitive-mutant telomere binding protein Cdc13p, we demonstrate that inhibition of TOR (Target of Rapamycin), a central regulator of nutrient pathways for cell growth, prevents cell death, but not growth arrest, induced by inactivation of Cdc13-1p. This function of TOR is novel and separable from its G1 inhibition function, and not associated with alterations in the telomere length, the amount of G-tails, and the telomere position effect (TPE) in cdc13-1 cells. Furthermore, antioxidants were also shown to prevent cell death initiated by inactivation of cdc13-1. Moreover, inhibition of TOR was also shown to prevent cell death induced by inactivation of telomerase in an est1 mutant. Interestingly, rapamycin did not prevent cell death induced by DNA damaging agents such as etoposide and UV. In the aggregate, our results suggest that the TOR signaling pathway is specifically involved in the regulation of cell death initiated by telomere dysfunction.
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- 2008
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23. Supplementary Figures 1-6, Table 1 from G-Quadruplexes Induce Apoptosis in Tumor Cells
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Leroy F. Liu, Daniel S. Pilch, Christopher M. Barbieri, Yongjie Chen, Yuan-Chin Tsai, Angela A. Liu, Laurence M. Wood, Xuan Fu, Chao-Po Lin, and Haiyan Qi
- Abstract
Supplementary Figures 1-6, Table 1 from G-Quadruplexes Induce Apoptosis in Tumor Cells
- Published
- 2023
24. Data from G-Quadruplexes Induce Apoptosis in Tumor Cells
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Leroy F. Liu, Daniel S. Pilch, Christopher M. Barbieri, Yongjie Chen, Yuan-Chin Tsai, Angela A. Liu, Laurence M. Wood, Xuan Fu, Chao-Po Lin, and Haiyan Qi
- Abstract
Several G-rich oligodeoxynucleotides (ODNs), which are capable of forming G-quadruplexes, have been shown to exhibit antiproliferative activity against tumor cell lines and antitumor activity in nude mice carrying prostate and breast tumor xenografts. However, the molecular basis for their antitumor activity remains unclear. In the current study, we showed that a variety of telomeric G-tail oligodeoxynucleotides (TG-ODNs) exhibited antiproliferative activity against many tumor cells in culture. Systematic mutational analysis of the TG-ODNs suggests that the antiproliferative activity depends on the G-quadruplex conformation of these TG-ODNs. TG-ODNs were also shown to induce poly(ADP-ribose) polymerase-1 cleavage, phosphatidylserine flipping, and caspase activation, indicative of induction of apoptosis. TG-ODN–induced apoptosis was largely ataxia telangiectasia mutated (ATM) dependent. Furthermore, TG-ODN–induced apoptosis was inhibited by the c-Jun NH2-terminal kinase (JNK) inhibitor SP600125. Indeed, TG-ODNs were shown to activate the JNK pathway in an ATM-dependent manner as evidenced by elevated phosphorylation of JNK and c-Jun. Interestingly, a number of G-quadruplex ODNs (GQ-ODN) derived from nontelomeric sequences also induced ATM/JNK-dependent apoptosis, suggesting a possible common mechanism of tumor cell killing by GQ-ODNs. (Cancer Res 2006; 66(24): 11808-16)
- Published
- 2023
25. Mafic magma recharge triggered eruption of a long-lived shallow silicic magma reservoir beneath a submarine volcano from the southwestern Okinawa Trough
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Zuxing Chen, Landry Soh Tamehe, Yuxiang Zhang, Shuai Chen, Zhigang Zeng, Xuebo Yin, Xiaoyuan Wang, Haiyan Qi, and Mingjiang Cai
- Subjects
Geochemistry and Petrology ,Geology - Published
- 2023
26. Lithium, Oxygen and Magnesium Isotope Systematics of Volcanic Rocks in the Okinawa Trough: Implications for Plate Subduction Studies
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Zhigang Zeng, Xiaohui Li, Yuxiang Zhang, and Haiyan Qi
- Subjects
western Pacific ,Li-O-Mg isotopes ,magma ,plate subduction ,Okinawa Trough ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Ocean Engineering ,GC1-1581 ,Oceanography ,Water Science and Technology ,Civil and Structural Engineering - Abstract
Determining the influence of subduction input on back-arc basin magmatism is important for understanding material transfer and circulation in subduction zones. Although the mantle source of Okinawa Trough (OT) magmas is widely accepted to be modified by subducted components, the role of slab-derived fluids is poorly defined. Here, major element, trace element, and Li, O and Mg isotopic compositions of volcanic lavas from the middle OT (MOT) and southern OT (SOT) were analyzed. Compared with the MOT volcanic lavas, the T9-1 basaltic andesite from the SOT exhibited positive Pb anomalies, significantly lower Nd/Pb and Ce/Pb ratios, and higher Ba/La ratios, indicating that subducted sedimentary components affected SOT magma compositions. The δ7Li, δ18O, and δ26Mg values of the SOT basaltic andesite (−5.05‰ to 4.98‰, 4.83‰ to 5.80‰ and −0.16‰ to −0.09‰, respectively) differed from those of MOT volcanic lavas. Hence, the effect of the Philippine Sea Plate subduction component, (low δ7Li and δ18O and high δ26Mg) on magmas in the SOT was clearer than that in the MOT. This contrast likely appears because the amounts of fluids and/or melts derived from altered oceanic crust (AOC, lower δ18O) and/or subducted sediment (lower δ7Li, higher δ18O and δ26Mg) injected into magmas in the SOT are larger than those in the MOT and because the injection ratio between subducted AOC and sediment is always >1 in the OT. The distance between the subducting slab and overlying magma may play a significant role in controlling the differences in subduction components injected into magmas between the MOT and SOT.
- Published
- 2022
27. Integration detection of mercury(<scp>ii</scp>) and GSH with a fluorescent 'on-off-on' switch sensor based on nitrogen, sulfur co-doped carbon dots
- Author
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Haiyan Qi, Xiaona Sun, Tao Jing, Jinlong Li, and Jun Li
- Subjects
General Chemical Engineering ,General Chemistry - Abstract
NS-CDs are employed as a sensitive sensor for the integration detection of Hg2+ and GSH. Hg2+ effectively quenching the fluorescence by static quenching. GSH is able to recover the fluorescence owing to the stronger binding between Hg2+ and GSH.
- Published
- 2022
28. Transforming waste into value: pomelo-peel-based nitrogen-doped carbon dots for the highly selective detection of tetracycline
- Author
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Haiyan Qi, Demin Huang, Jing Jing, Maoxia Ran, Tao Jing, Ming Zhao, Chenqi Zhang, Xiaona Sun, Rokayya Sami, and Nada Benajiba
- Subjects
General Chemical Engineering ,General Chemistry - Abstract
Tetracycline (TC) is widely used as a veterinary drug, and its residue in livestock products could enter the human body and cause damage. In this study, we developed an eco-friendly approach that utilized pomelo peel as a carbon source to synthesize new water-soluble N-doped carbon dots (P-NCDs) with blue fluorescence, obtaining a high quantum yield of up to 76.47% and achieving the goal of turning waste into value. Our prepared P-NCDs can selectively recognized TC, and their fluorescence was quenched based on the IFE. P-NCDs could measure the TC concentration in the linear range of 0-100 μmol L
- Published
- 2022
29. Preparation of Bi/BiOBr sensitized titania nanorod arrays via a one-pot solvothermal method and construction of kanamycin photoelectrochemical aptasensors
- Author
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Min Wu, Mengyao Dong, Zeinhom M. El-Bahy, Tao Jing, Gaber A. M. Mersal, Jingzhi Tian, Haiyan Qi, Danni Shi, Nithesh Naik, Vignesh Murugadoss, Mohamed M. Ibrahim, Mina Huang, and Zhanhu Guo
- Subjects
Inorganic Chemistry - Abstract
Photoactive Bi/BiOBr/TiO2 NRA composites displayed an efficient visible light response and fast charge transport rate and were used to construct sensitive kanamycin photoelectrochemical aptasensors.
- Published
- 2022
30. Identification and verification of circRNA biomarkers for coronary artery disease based on WGCNA and the LASSO algorithm
- Author
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Qilong Zhong, Shaoyue Jin, Zebo Zhang, Haiyan Qian, Yanqing Xie, Peiling Yan, Wenming He, and Lina Zhang
- Subjects
Coronary artery disease ,Circular RNA ,Biomarker ,WGCNA ,LASSO ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background The role of circular RNAs (circRNAs) as biomarkers of coronary artery disease (CAD) remains poorly explored. This study aimed to identify and validate potential circulating circRNAs as biomarkers for the diagnosis of CAD. Methods The expression profile of circRNAs associated with CAD was obtained from Gene Expression Omnibus (GEO) database. Differential expression analysis, weighted gene co-expression network analysis (WGCNA) and least absolute shrinkage and selection operation (LASSO) were employed to identify CAD-related hub circRNAs. The expression levels of these hub circRNAs were validated using qRT-PCR in blood samples from 100 CAD patients and 100 controls. The diagnostic performance of these circRNAs was evaluated through logistic regression analysis, receiver operator characteristic (ROC) analysis, integrated discrimination improvement (IDI), and net reclassification improvement (NRI). Functional enrichment analyses were performed to predict the possible mechanisms of circRNAs in CAD. Results A total of ten CAD-related hub circRNAs were identified through WGCNA and LASSO analysis. Among them, hsa_circ_0069972 and hsa_circ_0021509 were highly expressed in blood samples of CAD patients, and they were identified as independent predictors after adjustment for relevant confounders. The area under the ROC curve for hsa_circ_0069972 and hsa_circ_0021509 was 0.760 and 0.717, respectively. The classification of patients was improved with the incorporation of circRNAs into the clinical model composed of conventional cardiovascular risk factors, showing an IDI of 0.131 and NRI of 0.170 for hsa_circ_0069972, and an IDI of 0.111 and NRI of 0.150 for hsa_circ_0021509. Functional enrichment analyses revealed that the hsa_circ_0069972-miRNA-mRNA network was enriched in TGF-β、FoxO and Hippo signaling pathways, while the hsa_circ_0021509-miRNA-mRNA network was enriched in PI3K/Akt and MAPK signaling pathways. Conclusion Hsa_circ_0069972 and hsa_circ_0021509 were identified by integrated analysis, and they are highly expressed in CAD patients. They may serve as novel biomarkers for CAD.
- Published
- 2024
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31. Mineralogy, geochemistry, and sulfur isotope characteristics of sediment-hosted hydrothermal sulfide minerals from the southern Okinawa Trough
- Author
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Haiyan Qi, Zhigang Zeng, Xuebo Yin, Yang Yamin, Shuai Chen, Xiaoyuan Wang, Bowen Zhu, and Zuxing Chen
- Subjects
Mineral ,Chemistry ,Geochemistry ,Mineralogy ,Aquatic Science ,engineering.material ,Oceanography ,Sulfide minerals ,Hydrothermal circulation ,δ34S ,Sphalerite ,Galena ,engineering ,Pyrite ,Pyrrhotite - Abstract
Studies of the mineralogy and sulfur isotope composition of sediment-hosted hydrothermal sulfide minerals in cores are important for understanding the seafloor mineralization environment and material source and reconstructing the hydrothermal history. However, the source of ore-forming materials and the history of hydrothermal activity in the southern Okinawa Trough (SOT) remain unclear. Here, the mineralogy and sulfur isotope characteristics of sulfides from gravity core HOBAB4-S2, collected between the Yonaguni Knoll IV hydrothermal field (HF) and the Tangyin HF, was investigated. Enrichments in Zn (up to 321×10−6), Cu (up to 73.7×10−6), and Pb (up to 160×10−6) and the presence of pyrite, galena, pyrrhotite and minor sphalerite and chalcopyrite provide evidence for the input of hydrothermal materials into the sediments. The pyrite morphologies include disseminated cubic, pentagonal dodecahedron, and framboidal forms. Except for minor framboidal pyrite, euhedral pyrite from core HOBAB4-S2 is mainly of hydrothermal origin with Co/Ni ratios >1 and S/Fe atomic ratios
- Published
- 2021
32. Identification of a Seven-lncRNA-mRNA Signature for Recurrence and Prognostic Prediction in Relapsed Acute Lymphoblastic Leukemia Based on WGCNA and LASSO Analyses
- Author
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Jianping Lan, Haiyan Qi, Long Chi, Xiaogang Wang, Xing Jin, and Wensong Wang
- Subjects
0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Article Subject ,Lymphoblastic Leukemia ,Biology ,Pathology and Forensic Medicine ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,Lasso (statistics) ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,RNA, Messenger ,Childhood Acute Lymphoblastic Leukemia ,Gene ,RC254-282 ,Messenger RNA ,QH573-671 ,Receiver operating characteristic ,business.industry ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cell Biology ,General Medicine ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Prognosis ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,030220 oncology & carcinogenesis ,Molecular Medicine ,RNA, Long Noncoding ,Cytology ,business ,Research Article - Abstract
Abnormal expressions of long noncoding RNAs (lncRNAs) and protein-encoding messenger RNAs (mRNAs) are important for the development of childhood acute lymphoblastic leukemia (ALL). This study developed an lncRNA-mRNA integrated classifier for the prediction of recurrence and prognosis in relapsed childhood ALL by using several transcriptome data. Weighted gene coexpression network analysis revealed that green, turquoise, yellow, and brown modules were preserved across the TARGET, GSE60926, GSE28460, and GSE17703 datasets, and they were associated with clinical relapse and death status. A total of 184 genes in these four modules were differentially expressed between recurrence and nonrecurrence samples. Least absolute shrinkage and selection operator analysis showed that seven genes constructed a prognostic signature (including one lncRNA: LINC00652 and six mRNAs: INSL3, NIPAL2, REN, RIMS2, RPRM, and SNAP91). Kaplan-Meier curve analysis observed that patients in the high-risk group had a significantly shorter overall survival than those of the low-risk group. Receiver operating characteristic curve analysis demonstrated that this signature had high accuracy in predicting the 5-year overall survival and recurrence outcomes, respectively. LINC00652 may function by coexpressing with the above prognostic genes (INSL3, SNAP91, and REN) and lipid metabolism-related genes (MIA2, APOA1). Accordingly, this lncRNA-mRNA-based classifier may be clinically useful to predict the recurrence and prognosis for childhood ALL. These genes represent new targets to explain the mechanisms for ALL.
- Published
- 2021
33. [Identifying SARS-CoV-2 main protease inhibitors by a novel sandwich-like fluorescence polarization screening assay]
- Author
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Haohao, Yan, Gangan, Yan, Haiyan, Qi, Zhicheng, Liu, Xiaoli, Liu, Xiaoping, Liu, Ni, Li, and Yunyu, Chen
- Subjects
Biological Products ,SARS-CoV-2 ,Research ,Endopeptidases ,COVID-19 ,Humans ,Coronavirus 3C Proteases ,Peptide Hydrolases - Abstract
SARS-CoV-2 main protease (Mpro) is responsible for polyprotein cleavage to release non-structural proteins (nsps) for viral genomic RNA replication, and its homologues are absent in human cells. Therefore, Mpro has been regarded as one of the ideal drug targets for the treatment of coronavirus disease 2019 (COVID-19). In this study, we first combined the fluorescence polarization (FP) technique with biotin-avidin system (BAS) to develop a novel sandwich-like FP screening assay for quick discovery of SARS-CoV-2 Mpro inhibitors from a natural product library. With this screening assay, anacardic acid (AA) and 1, 2, 3, 4, 6
- Published
- 2022
34. [Discovery of SARS-CoV-2 main protease inhibitors using an optimized FRET-based high-throughput screening assay]
- Author
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Gangan, Yan, Dongsheng, Li, Haiyan, Qi, Zhenghao, Fu, Xiaoping, Liu, Jing, Zhang, and Yunyu, Chen
- Subjects
SARS-CoV-2 ,Endopeptidases ,Escherichia coli ,Fluorescence Resonance Energy Transfer ,COVID-19 ,Humans ,Reproducibility of Results ,Protease Inhibitors ,Coronavirus 3C Proteases ,High-Throughput Screening Assays - Abstract
For rapid discovery of novel SARS-CoV-2 main protease (Mpro) inhibitors, an optimized fluorescence resonance energy transfer (FRET)-based high-throughput screening (HTS) assay was developed. The recombinant Mpro was expressed in
- Published
- 2022
35. MSC-AS1 induced cell growth and inflammatory mediators secretion through sponging miR-142-5p/DDX5 in gastric carcinoma
- Author
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Haiyan Qi, Yan Liu, Lin Li, Da Chen, Xiaoxu Wu, Yan Gao, and Yanqi Li
- Subjects
Aging ,miR-142-5p ,Mutant ,medicine.disease_cause ,DEAD-box RNA Helicases ,Downregulation and upregulation ,Stomach Neoplasms ,medicine ,DDX5 ,Humans ,Luciferase ,Secretion ,MSC-AS1 ,Cell Proliferation ,Oncogene ,Chemistry ,Cell growth ,gastric cancer ,Carcinoma ,Cancer ,Cell Biology ,medicine.disease ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Cancer research ,RNA, Long Noncoding ,Inflammation Mediators ,Carcinogenesis ,Research Paper - Abstract
Emerging studies have noted that dysregulated lncRNAs are implicated in cancer progression and tumorigenesis. We first showed that MSC-AS1 was overexpressed in gastric cancer (GC) cells (HGC-27, MKN-45, SGC-7901 and MGC-803 cells) compared with GES cells. We observed that MSC-AS1 was upregulated in GC specimens compared with paired normal specimens. MSC-AS1 increased cell growth and cycle progression. Moreover, the overexpression of MSC-AS1 enhanced the secretion of the inflammatory mediators IL-1β, IL-6 and TNF-α. We found that the overexpression of MSC-AS1 inhibited the expression of miR-142-5p in HGC-27 cells. We noted that DDK5 was a target gene of miR-142-5p. The overexpression of miR-142-5p suppressed the luciferase activity of wild-type DDX5, but the luciferase activity of the mutant DDX5 was not changed. We showed that miR-142-5p was downregulated in GC specimens compared with paired normal specimens. MSC-AS1 expression was inversely correlated with miR-142-5p expression in GC specimens. MSC-AS1 induced cell growth, cell cycle progression and inflammatory mediator secretion by modulating DDX5. These results showed that MSC-AS1 functions as a key oncogene in the development of GC.
- Published
- 2021
36. Tetrastigma hemsleyanum (Sanyeqing) root extracts evoke S phase arrest while inhibiting the migration and invasion of human pancreatic cancer PANC-1 cells
- Author
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Yifan Sun, Haiyan Qin, Chunchun Zhang, Jian Xu, and Ting Zhang
- Subjects
Tetrastigma hemsleyanum ,GC-MS ,PANC-1 cell ,Migration ,Invasion ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background Ethyl acetate extracts from Tetrastigma hemsleyanum (Sanyeqing) (EFT), a member of the Vitaceae plant family, have been shown to exhibit efficacy against a variety of cancers. In this light, our current study seeks to examine the mechanism of efficacy between EFT extracts and human pancreatic cancer PANC-1 cells. Methods The chemical components of EFT were analyzed by gas chromatography–mass spectrometry. The cytotoxicity of EFT on PANC-1 cells was measured using an MTT assay. In order to investigate EFT induction of cell cycle arrest, changes in cell-cycle distribution were monitored by flow cytometry. Wound healing and transwell assays were employed to investigate whether migration and invasion of PANC-1 cells were inhibited by EFT. Relative protein expression was detected using Western blot. Results GC-MS analysis of the chemical composition of EFT revealed that the majority of constituents were organic acids and their corresponding esters. EFT exhibits measurable cytotoxicity and inhibition of PANC-1 invasion. Growth inhibition was primarily attributed to downregulation of CDK2 which induces cell cycle arrest in the S-phase. Inhibition of metastasis is achieved through downregulation of mesenchymal-associated genes/activators, including ZEB1, N-cadherin, Vimentin, and Fibronectin. Meanwhile, the expression of E-cadherin was significantly increased by EFT treatment. Furthermore, downregulation of MMP-2 and MMP-9 were observed. Conclusion Treatment of PANC-1 with EFT demonstrated measurable cytotoxic effects. Furthermore, EFT evoked S phase arrest while inhibiting the migration and invasion of PANC-1 cells. Additionally, EFT inhibited the epithelial to mesenchymal transition and MMPs expression in PANC-1 cells. This study serves to confirm the strong therapeutic potential of EFT while identifying the mechanisms of action.
- Published
- 2024
- Full Text
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37. NLRC4 methylation and its response to intravenous immunoglobulin therapy in Kawasaki disease: a case control study
- Author
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Beirong Yu, Bangxu Zheng, Yu Shen, Yijing Shen, Haiyan Qiu, Ling Wu, Yuanling Chen, Xiaohong Cai, Junhua Wu, and Qingxiao Hong
- Subjects
Kawasaki disease ,NLRC4 ,Methylation ,Intravenous immunoglobulin ,Pediatrics ,RJ1-570 - Abstract
Abstract Background Kawasaki disease (KD) is a systemic vasculitis accompanied by many systemic physiological and biochemical changes. Elucidating its molecular mechanisms is crucial for diagnosing and developing effective treatments. NLR Family CARD Domain Containing 4 (NLRC4) encodes the key components of inflammasomes that function as pattern recognition receptors. The purpose of this study was to investigate the potential of NLRC4 methylation as a biomarker for KD. Methods In this study, pyrosequencing was utilized to analyze NLRC4 promoter methylation in blood samples from 44 children with initial complete KD and 51 matched healthy controls. Methylation at five CpG sites within the NLRC4 promoter region was evaluated. Results Compared to controls, NLRC4 methylation significantly decreased in KD patients (CpG1: p = 2.93E-06; CpG2: p = 2.35E-05; CpG3: p = 6.46E-06; CpG4: p = 2.47E-06; CpG5: p = 1.26E-05; average methylation: p = 5.42E-06). These changes were significantly reversed after intravenous immunoglobulin (IVIG) treatment. ROC curve analysis demonstrated remarkable diagnostic capability of mean NLRC4 gene methylation for KD (areas under ROC curve = 0.844, sensitivity = 0.75, p = 9.61E-06, 95% confidence intervals were 0.762–0.926 for mean NLRC4 methylation). In addition, NLRC4 promoter methylation was shown to be significantly negatively correlated with the levels of central granulocyte percentage, age, mean haemoglobin quantity and mean erythrocyte volume. Besides, NLRC4 promoter methylation was positively correlated with lymphocyte percentage, lymphocyte absolute value. Conclusions Our work revealed the role of peripheral NLRC4 hypomethylation in KD pathogenesis and IVIG treatment response, could potentially serve as a treatment monitoring biomarker, although its precise functions remain to be elucidated.
- Published
- 2024
- Full Text
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38. Effects of Ropivacaine Nanoparticles on the Apoptosis of Cerebral Vascular Endothelial Cells
- Author
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Wei Liu, Yajun Liu, Duan-Yu Wang, and Haiyan Qi
- Subjects
Male ,Materials science ,Biomedical Engineering ,Ischemia ,Apoptosis ,Bioengineering ,Pharmacology ,Blood–brain barrier ,Brain Ischemia ,Brain ischemia ,Edema ,medicine ,Animals ,Ropivacaine ,General Materials Science ,Rats, Wistar ,Penumbra ,Endothelial Cells ,General Chemistry ,Condensed Matter Physics ,medicine.disease ,Rats ,Endothelial stem cell ,medicine.anatomical_structure ,Blood-Brain Barrier ,Nanoparticles ,medicine.symptom ,medicine.drug - Abstract
To investigate the protective effect of ropivacaine nanoparticles on endothelial cells in the blood brain barrier (BBB) during the development of ischemic brain edema, and its effects on endothelial cell death. Forty-two male Wistar rats weighing 250–300 g and aged 3–4 months were randomly divided into three groups: (1) ropivacaine nanoparticles, (2) saline control and (3) sham operation groups. The membrane of capillary endothelial cells in the animals treated with ropivacaine nanoparticles were intact, with reduced edema, and less severe brain injury when compared to the control. In the ropivacaine nanoparticle group, the number of apoptotic cells decreased at 6 h and 24 h after ischemia, while the number of apoptotic cells in the ischemic penumbra increased. The number of apoptotic cells in the ropivacaine nanoparticles group was significantly lower than in the saline treated control. Ropivacaine nanoparticles exert significant protective effects on the vascular endothelial cells and the BBB during cerebral ischemia.
- Published
- 2020
39. A Continuously Tunable Full-Color Emission Nitrogen-Doped Carbon Dots and for Ultrasensitive and Highly Selective Detection of Ascorbic Acid
- Author
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Demin Huang, Haiyan Qi, Jing Jing, Rokayya Sami, Tao Jing, Sultan J. Alsufyani, Nada Benajiba, and Nawal Madkhali
- Subjects
General Chemical Engineering ,General Materials Science ,nitrogen-doped carbon dots ,full-color emission ,ascorbic acid ,fluorescence detection - Abstract
Nitrogen-doped carbon dots exhibiting excitation-dependent full-color emissions (F-NCDs) were prepared via the one-step hydrothermal method with citric acid and phenylenediamine. Specifically, the emission wavelength of the F-NCDs tuned from 452 nm to 602 nm due to the introduction of new energy levels by C=O and C=N functional groups. We exploited its stability in illumination, ionic strength, and pH, as well as its specificity, sensitivity, especially in ascorbic acid (AA) detection. F-NCDs could measure the AA concentration in the linear ranges of 0~0.1 and 0.1~1 mmol/L with the detection limit (LOD, S/N = 3) as low as 2.6 nmol/L. Additionally, we successfully detected AA in bovine serum with our F-NCDs and obtained the result within 1 min. Because of full-color emission features, we believe our F-NCDs have a great potential in fluorescent sensor detection.
- Published
- 2021
40. ELANE is a promising prognostic biomarker that mediates pyroptosis in gastric cancer
- Author
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Ming Cui, Xiaowu Wang, Haiyan Qiao, Shixi Wu, and Bingbing Shang
- Subjects
ELANE ,Gastric cancer ,Pyroptosis ,Immune cells ,Clinical prognosis ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Gastric cancer (GC) is a typical malignant tumor and the main cause of cancer-related deaths. Its pathogenesis involves multiple steps, including pyroptosis, although these steps are still uncertain. Pyroptosis, also known as gasdermin-mediated programmed necrosis, participates in various pathological processes in tumors, including GC. ELANE, which encodes neutrophil elastase, is closely associated with GC. Additionally, ELANE has been implicated in GC cell pyroptosis, but this has not been confirmed. Therefore, investigating the link between ELANE and pyroptosis in GC is warranted. This research uses bioinformatics and experiments to examine the relationship between ELANE, pyroptosis, and GC prognosis. Methods: The GEO and TCGA databases, along with pyroptosis-related genes, were applied to identify pyroptosis-related differentially expressed genes (DEGs). ELANE was selected via primary screening. Using the median expression level of ELANE as the threshold, pyroptosis-related DEGs were divided into low- and high-ELANE groups. Based on the DEGs in these two groups, GO, KEGG and GSEA analyses were conducted to elucidate the mechanisms of ELANE in GC. Furthermore, we plotted ROC and Kaplan–Meier curves to analyze the clinical and pathological features of ELANE expression. The Nomograms tool was applied to calculate the predictive value of ELANE for the clinical outcomes of GC cases. Immunohistochemical analysis was performed to detect the level of ELANE in GC tissues and to validate whether ELANE was involved in pyroptosis in GC cells through cell experiments. Finally, the immune infiltration of ELANE was investigated, and interaction networks (proteins-ELANE, microRNA-ELANE, and small-molecule drug-ELANE) were constructed. Results: We aimed to investigate the expression of the ELANE gene in GC and study the relationship among ELANE, pyroptosis, and the prognosis of patients with GC. Differential expression analysis of gene-expression datasets from TCGA-STAD and GSE49051 revealed that the expression of the ELANE gene was significantly up-regulated in GC. Using STRING network analysis, we identified multiple proteins involved in the occurrence and development of GC, including interactions between ELANE and GSDMC, a member of the gasdermin protein family. Survival analysis showed that ELANE expression levels significantly affected overall survival (OS), disease-free survival (DFS), and progression-free survival (PFS) in patients with GC. Additionally, ROC analysis demonstrated that ELANE was effective in distinguishing GC patients from normal controls (AUC = 0.812). Immunohistochemical analysis showed that ELANE was highly expressed in gastric cancer tissues and was closely related to age, tumor grade, and stage. The cell experiments further confirmed that the high expression of ELANE in gastric cancer cells was associated with pyroptosis. Comprehensive analysis indicated that ELANE could be used as a potential prognostic marker for GC and plays an important role in pyroptosis. Conclusion: High ELANE expression is related to poor survival and prognosis of patients with GC. It participates in pyroptosis and immune infiltration in GC. Therefore, ELANE is a promising prognostic biomarker for pyroptosis in GC.
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- 2024
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41. A note on heat kernel of graphs
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Yang Yang, Wei Ke, Zhe Wang, and Haiyan Qiao
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Heat kernel ,Almost equitable partition ,Principal submatrix ,Laplacian matrix ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Consider a simple undirected connected graph G, with D(G) and A(G) representing its degree and adjacency matrices, respectively. Furthermore, L(G)=D(G)−A(G) is the Laplacian matrix of G, and Ht=exp(−tL(G)) is the heat kernel (HK) of G, with t>0 denoting the time variable. For a vertex u∈V(G), the uth element of the diagonal of the HK is defined as Ht(u,u)=(exp(−tL(G)))uu=∑k=0∞((−tL(G))k)uuk!, and HE(G)=∑i=1ne−tλi=∑u=1nHt(u,u) is the HK trace of G, where λ1,λ2,⋯,λn denote the eigenvalues of L(G). This study provides new computational formulas for the HK diagonal entries of graphs using an almost equitable partition and the Schur complement technique. We also provide bounds for the HK trace of the graphs.
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- 2024
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42. Dispersion and Intersection of Hydrothermal Plumes in the Manus Back-Arc Basin, Western Pacific
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Yunchao Shu, Xiaohui Li, Zhigang Zeng, Berit Lehrmann, Bramley J. Murton, Xiaoyuan Wang, Zuxing Chen, and Haiyan Qi
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QE1-996.5 ,Article Subject ,010504 meteorology & atmospheric sciences ,Geochemistry ,Geology ,010502 geochemistry & geophysics ,01 natural sciences ,Hydrothermal circulation ,Seafloor spreading ,Plume ,Current (stream) ,Water column ,Back-arc basin ,General Earth and Planetary Sciences ,Seawater ,Turbidity ,0105 earth and related environmental sciences - Abstract
The composition of hydrothermal plumes reflects the physical and chemical characteristics of seafloor hydrothermal fluids, which in turn reflects the host rock and subseafloor reaction conditions as well as the water column processes that act to alter the plumes as they disperse and age. Here, we show that the turbidity, current, pH value, dissolved Fe (dFe), and dissolved Mn (dMn) compositions of hydrothermal plumes can be used to understand the spatial distribution and source of hydrothermal systems in the submarine geological environment. Data were obtained from 18 hydrocast stations, among which the water column samples were collected at 8 stations during the MANUS cruise of R/V KEXUE in 2015. The results showed that the Satanic Mills plume and Fenway plume rose approximately 140 m and 220 m above the seafloor, respectively. In the Satanic Mills plume, dFe remained longer than dMn during lateral plume dispersal. There was a clear intersection of the Satanic Mills plume and Fenway plume between 1625 m and 1550 m in the PACMANUS hydrothermal field, and the varied dispersion trends of the mixed plumes were affected by current velocities at different depths. The physical and chemical properties of the seawater columns in the Manus Basin were affected by the input of high-Mn, high-Fe, and low-Mg vent fluids. The turbidity and dFe, dMn, and dissolved Mg concentrations in the sections of the plumes proximal to the Satanic Mills, Fenway, and Desmos vent sites were generally higher (turbidity, Mn, and Fe) and lower (Mg) than those in the sections of the plumes that were more distal from the vent sites. This implied that the plumes proximal to their vent fluid sources, which were interpreted to have relatively young ages, dispersed chemically over time, and their concentrations became more similar to those of the plumes that were more distal from their vent fluid sources.
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- 2020
43. Amphibole perspective to unravel the rhyolite as a potential fertile source for the Yonaguni Knoll IV hydrothermal system in the southwestern Okinawa Trough
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Yuxiang Zhang, Haiyan Qi, Xiaoyuan Wang, Yao Ma, Shuai Chen, Zuxing Chen, Wenqiang Yang, Zhigang Zeng, and Xuebo Yin
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Rhyolite ,Trough (geology) ,Geochemistry ,Geology ,Magma chamber ,Hydrothermal circulation ,Amphibole - Published
- 2019
44. [Preparation and identification of rat polyclonal antibody against SARS-CoV-2 main protease (Mpro)]
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Gangan, Yan, Haiyan, Qi, Haohao, Yan, Zhenghao, Fu, Xiaoping, Liu, and Yunyu, Chen
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SARS-CoV-2 ,Blotting, Western ,Animals ,COVID-19 ,Humans ,Antibodies ,Peptide Hydrolases ,Rats - Abstract
Objective To investigate the immunological functions of SARS-CoV-2 main protease (Mpro) in coronavirus disease 2019 (COVID-19), polyclonal antibody against Mpro was developed. Methods A codon-optimized SARS-CoV-2 Mpro gene was synthesized and ligated into a pET-28a vector for construction of a recombinant plasmid named by pET-28a-Mpro. Subsequently, this plasmid was transformed into E.coli Rosetta (DE3) competent cells for Mpro expression in an optimized condition, and then Mpro was purified using a HisTrap chelating column. The purified Mpro was used as immunogen to inoculate rats and the serum was collected after third immunization cycle. The titer, selectivity and sensitivity of polyclonal antibody against Mpro were analyzed using the ELISA and Western blot analysis. Results An optimized expression condition in E.coli cells for Mpro was determined, and the recombinant Mpro was purified by a HisTrap chelating column. The ELISA and Western blot analysis demonstrated that the highly sensitive polyclonal antibody against Mpro specially recognized the recombinant Mpro, and the titer reached 1:256 000. Conclusion The highly specific polyclonal antibody against SARS-CoV-2 Mpro is successfully prepared, which lays an experimental foundation for investigating the immunological function of Mpro in COVID-19.
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- 2021
45. Preparation of Eu0.075Tb0.925-Metal Organic Framework as a Fluorescent Probe and Application in the Detection of Fe3+ and Cr2O72−
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Haiyan Qi, Hongtao Chu, Minggang Hu, Shili Qin, and Jie Yin
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Diffraction ,Luminescence ,Materials science ,Cr2O72− ,Analytical chemistry ,luminous sensing ,TP1-1185 ,Ligands ,Biochemistry ,Article ,Ln-MOFs ,Analytical Chemistry ,X-Ray Diffraction ,X-ray photoelectron spectroscopy ,Electrical and Electronic Engineering ,Fe3+ ,Instrumentation ,Metal-Organic Frameworks ,Fluorescent Dyes ,Detection limit ,Ligand ,Chemical technology ,Fluorescence ,Atomic and Molecular Physics, and Optics ,Linear range ,Metal-organic framework - Abstract
Luminescent Ln-MOFs (Eu0.075Tb0.925-MOF) were successfully synthesised through the solvothermal reaction of Tb(NO3)3·6H2O, Eu(NO3)3·6H2O, and the ligand pyromellitic acid. The product was characterised by X-ray diffraction (XRD), TG analysis, EM, X-ray photoelectron spectroscopy (XPS), and luminescence properties, and results show that the synthesised material Eu0.075Tb0.925-MOF has a selective ratio-based fluorescence response to Fe3+ or Cr2O72−. On the basis of the internal filtering effect, the fluorescence detection experiment shows that as the concentration of Fe3+ or Cr2O72− increases, the intensity of the characteristic emission peak at 544 nm of Tb3+ decreases, and the intensity of the characteristic emission peak at 653 nm of Eu3+ increases in Eu0.075Tb0.925-MOF. The fluorescence intensity ratio (I653/I544) has a good linear relationship with the target concentration. The detection linear range for Fe3+ or Cr2O72− is 10–100 μM/L, and the detection limits are 2.71 × 10−7 and 8.72 × 10−7 M, respectively. Compared with the sensor material with a single fluorescence emission, the synthesised material has a higher anti-interference ability. The synthesised Eu0.075Tb0.925-MOF can be used as a highly selective and recyclable sensing material for Fe3+ or Cr2O72−. This material should be an excellent candidate for multifunctional sensors.
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- 2021
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46. Two kinds of biomass-derived carbon dots with one-step synthesis for Fe3+ and tetracyclines detection
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Haiyan Qi, Chuntong Liu, Jing Jing, Tao Jing, Xiaohong Zhang, Jinlong Li, Chao Luo, Lixin Qiu, and Qiuying Li
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Process Chemistry and Technology ,General Chemical Engineering - Published
- 2022
47. Convergence of tetraphenylethene towards a series of large size AIE compounds
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Yangyang Pan, Rui Zhu, Chaoran Qi, Anni Wang, Qi Jia, Cheng Lin, Haiyan Qi, Qinghai Zhou, Jingjing Xu, and Shengxiong Xiao
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Process Chemistry and Technology ,General Chemical Engineering - Published
- 2022
48. Bibliometric analysis of transforaminal lumbar interbody fusion: research status, trends, and future directions
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Xun Wang, Aiqi Zhang, Wenchao Yao, Haiyan Qiu, and Fabo Feng
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transforaminal lumbar interbody fusion ,tlif ,bibliometric analysis ,web of science ,knowledge structure ,Orthopedic surgery ,RD701-811 - Abstract
Purpose: Transforaminal lumbar interbody fusion (TLIF) is a classic surgical procedure for posterior lumbar fusion. This study aims to analyze the TLIF field by bibliometric method and comprehensively summarize the research status and trends. Methods: All TLIF-related articles were retrieved from the Web of Science. The data were analyzed using R software and SPSS to calculate corresponding indicators. Visualizations were drawn using VOSviewer and Scimago Graphica, including country, institution, journal, author, and keywords. Results: A total of 919 articles were included. The annual publication volume of TLIF-related articles presented an exponential growth. North America, Europe, and Asia were the main sources of articles, with the USA and China being the main contributors and the USA being the global research center for TLIF. The level of the national economy was an important factor affecting TLIF-related research. The highest number of contributions in this field was made by Kern Singh among authors and by Rush University among institutions. The European Spine Journal was the most influential journal. The research focus has gradually shifted from perfecting the TLIF technique toward emphasizing the patient level. The improvement of minimally invasive techniques and how to improve clinical outcomes as well as accelerate postoperative rehabilitation of patients may be the hot spot of future research. Conclusions: With the advancement of medical technology and the popularization of minimally invasive concepts in recent years, TLIF and its derivative technologies have attracted increasing attention. Patient-centered minimally invasive surgery is a hot research topic in the field of TLIF currently and will continue to be so into the future
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- 2023
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49. Iron, copper, and zinc isotopic fractionation in seafloor basalts and hydrothermal sulfides
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Nadine Mattielli, Shuai Chen, Haiyan Qi, Zhigang Zeng, Xiaohui Li, Bramley J. Murton, Jeroen de Jong, and Christopher R. Pearce
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010504 meteorology & atmospheric sciences ,Mid-ocean ridge basalts ,Geochemistry ,chemistry.chemical_element ,Zinc ,engineering.material ,010502 geochemistry & geophysics ,Oceanography ,01 natural sciences ,Hydrothermal circulation ,Magmatic processes and ore formation ,Geochemistry and Petrology ,Seafloor hydrothermal sulfides ,Océanographie physique et chimique ,Géologie ,0105 earth and related environmental sciences ,Basalt ,geography ,geography.geographical_feature_category ,Chalcopyrite ,Géochimie ,Pétrologie ,Geology ,Sulfide minerals ,Volcanic rock ,Sphalerite ,chemistry ,visual_art ,visual_art.visual_art_medium ,engineering ,Pyrite ,Fe-Cu-Zn isotopic fractionation - Abstract
Studies of the Fe, Cu, and Zn isotopic compositions of volcanic rocks and sulfides provide an important tool for understanding magmatic, hydrothermal, and alteration processes, thereby enabling the determination of both transition metal sources and the quantification of the petrologic environmental impacts of hydrothermal activities. In this study, the δ56Fe and δ57Fe values of the mid-ocean ridge basalts (MORBs) are higher than those of the seafloor hydrothermal fluids, while the reverse is true for the δ66Zn and δ68Zn values, suggesting that basalt-fluid interactions preferentially incorporate isotopically light Fe and heavy Zn into the fluids, resulting in the relative enrichment of heavier Fe and lighter Zn isotopes in altered basaltic rocks. Most of the δ56Fe values (−1.96 to +0.11‰) of the sulfide minerals are within the range of the vent fluids, but they are significantly lower than those of the MORBs and back-arc basin basalts (BABBs), suggesting that the Fe in the sulfides was mainly derived from the fluids. However, the majority of the chalcopyrite δ56Fe and δ57Fe values are higher than those of the sphalerite and pyrite. This suggests that high-temperature sulfide minerals are enriched in 56Fe and 57Fe, whereas medium- and low-temperature sulfides are depleted in 56Fe and 57Fe. Moreover, the δ65Cu (−0.88 to −0.16‰) and δ66Zn (−0.39 to −0.03‰) values of the sulfide minerals are significantly lower than those of the MORBs, BABBs, and fluids, suggesting that 63Cu and 64Zn were preferentially removed from the fluids and incorporated into the chalcopyrite and sphalerite, respectively. Consequently, vent fluid injection and deposition can cause the heavier Cu and Zn isotopic compositions of hydrothermal plumes, seawater, and sediments., SCOPUS: ar.j, info:eu-repo/semantics/published
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- 2021
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50. Smectite formation in metalliferous sediments near the East Pacific Rise at 13°N
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Xiaoyuan Wang, Yao Ma, Haiyan Qi, Kunbo Rong, Shuai Chen, Zhigang Zeng, and Xuebo Yin
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010504 meteorology & atmospheric sciences ,Terrigenous sediment ,Geochemistry ,Authigenic ,Aquatic Science ,engineering.material ,010502 geochemistry & geophysics ,Oceanography ,01 natural sciences ,Hydrothermal circulation ,chemistry.chemical_compound ,Isotope fractionation ,chemistry ,Illite ,engineering ,Kaolinite ,Clay minerals ,Chlorite ,0105 earth and related environmental sciences - Abstract
A 43 cm long E271 sediment core collected near the East Pacific Rise (EPR) at 13°N were studied to investigate the origin of smectite for understanding better the geochemical behavior of hydrothermal material after deposition. E271 sediments are typical metalliferous sediments. After removal of organic matter, carbonate, biogenic opal, and Fe-Mn oxide by a series of chemical procedures, clay minerals (
- Published
- 2018
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