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10 results on '"Haley Raphael"'

1. Supplemental Figure S5 from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma

Author

Kelly M. Bailey, Linda M. McAllister-Lucas, Peter C. Lucas, Rajen Mody, Katherine A. Janeway, Kurt Weiss, Nivitha Periyapatna, Haley Raphael, Chandan Kumar-Sinha, Michelle F. Jacobs, Nathanael G. Bailey, Claire M. Julian, Rosemarie E. Venier, Elina Mukherjee, Jessica D. Daley, and Lisa M. Maurer

Abstract

Loss of GBP1 expression does not impact PSaRC318 cell migration.

Published
2023
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2. Supplemental Table S2 from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma

Author

Kelly M. Bailey, Linda M. McAllister-Lucas, Peter C. Lucas, Rajen Mody, Katherine A. Janeway, Kurt Weiss, Nivitha Periyapatna, Haley Raphael, Chandan Kumar-Sinha, Michelle F. Jacobs, Nathanael G. Bailey, Claire M. Julian, Rosemarie E. Venier, Elina Mukherjee, Jessica D. Daley, and Lisa M. Maurer

Abstract

Prevalence of somatic DNA damage repair gene variants in Ewing cell lines included in original studies of PARP inhibition.

Published
2023
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3. Supplemental Figure S3 from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma

Author

Kelly M. Bailey, Linda M. McAllister-Lucas, Peter C. Lucas, Rajen Mody, Katherine A. Janeway, Kurt Weiss, Nivitha Periyapatna, Haley Raphael, Chandan Kumar-Sinha, Michelle F. Jacobs, Nathanael G. Bailey, Claire M. Julian, Rosemarie E. Venier, Elina Mukherjee, Jessica D. Daley, and Lisa M. Maurer

Abstract

Interferon-gamma-induced GBP1 expression in A673 Ewing sarcoma cells.

Published
2023
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4. Supplemental Figure S2 from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma

Author

Kelly M. Bailey, Linda M. McAllister-Lucas, Peter C. Lucas, Rajen Mody, Katherine A. Janeway, Kurt Weiss, Nivitha Periyapatna, Haley Raphael, Chandan Kumar-Sinha, Michelle F. Jacobs, Nathanael G. Bailey, Claire M. Julian, Rosemarie E. Venier, Elina Mukherjee, Jessica D. Daley, and Lisa M. Maurer

Abstract

BARD1 loss enhances Ewing sarcoma cell apoptosis in response to niraparib plus radiation in CHLA10 Ewing sarcoma cells.

Published
2023
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5. Data from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma

Author

Kelly M. Bailey, Linda M. McAllister-Lucas, Peter C. Lucas, Rajen Mody, Katherine A. Janeway, Kurt Weiss, Nivitha Periyapatna, Haley Raphael, Chandan Kumar-Sinha, Michelle F. Jacobs, Nathanael G. Bailey, Claire M. Julian, Rosemarie E. Venier, Elina Mukherjee, Jessica D. Daley, and Lisa M. Maurer

Abstract

Ewing sarcoma is a fusion oncoprotein–driven primary bone tumor. A subset of patients (∼10%) with Ewing sarcoma are known to harbor germline variants in a growing number of genes involved in DNA damage repair. We recently reported our discovery of a germline mutation in the DNA damage repair protein BARD1 (BRCA1-associated RING domain-1) in a patient with Ewing sarcoma. BARD1 is recruited to the site of DNA double stranded breaks via the PARP protein and plays a critical role in DNA damage response pathways including homologous recombination. We thus questioned the impact of BARD1 loss on Ewing cell sensitivity to DNA damage and the Ewing sarcoma transcriptome. We demonstrate that PSaRC318 cells, a novel patient-derived cell line harboring a pathogenic BARD1 variant, are sensitive to PARP inhibition and by testing the effect of BARD1 depletion in additional Ewing sarcoma cell lines, we confirm that BARD1 loss enhances cell sensitivity to PARP inhibition plus radiation. In addition, RNA-sequencing analysis revealed that loss of BARD1 results in the upregulation of GBP1 (guanylate-binding protein 1), a protein whose expression is associated with variable response to therapy depending on the adult carcinoma subtype examined. Here, we demonstrate that GBP1 contributes to the enhanced sensitivity of BARD1-deficient Ewing cells to DNA damage. Together, our findings demonstrate the impact of loss-of function mutations in DNA damage repair genes, such as BARD1, on Ewing sarcoma treatment response.Significance:This work provides preclinical support for the inclusion of pediatric patients with advanced Ewing sarcoma and pathogenic germline variants in BARD1 in future clinical trials testing novel agents inducing DNA damage/targeting DNA damage repair.

Published
2023
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6. Supplemental Table S1 from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma

Author

Kelly M. Bailey, Linda M. McAllister-Lucas, Peter C. Lucas, Rajen Mody, Katherine A. Janeway, Kurt Weiss, Nivitha Periyapatna, Haley Raphael, Chandan Kumar-Sinha, Michelle F. Jacobs, Nathanael G. Bailey, Claire M. Julian, Rosemarie E. Venier, Elina Mukherjee, Jessica D. Daley, and Lisa M. Maurer

Abstract

Benign germline BARD1 variants.

Published
2023
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7. Supplemental Figure S4 from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma

Author

Kelly M. Bailey, Linda M. McAllister-Lucas, Peter C. Lucas, Rajen Mody, Katherine A. Janeway, Kurt Weiss, Nivitha Periyapatna, Haley Raphael, Chandan Kumar-Sinha, Michelle F. Jacobs, Nathanael G. Bailey, Claire M. Julian, Rosemarie E. Venier, Elina Mukherjee, Jessica D. Daley, and Lisa M. Maurer

Abstract

PSaRC318 cell confluency data.

Published
2023
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8. Supplemental Figure S1 from BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma

Author

Kelly M. Bailey, Linda M. McAllister-Lucas, Peter C. Lucas, Rajen Mody, Katherine A. Janeway, Kurt Weiss, Nivitha Periyapatna, Haley Raphael, Chandan Kumar-Sinha, Michelle F. Jacobs, Nathanael G. Bailey, Claire M. Julian, Rosemarie E. Venier, Elina Mukherjee, Jessica D. Daley, and Lisa M. Maurer

Abstract

Additional protein/mRNA expression data (corresponding to Figure 2).

Published
2023
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9. Serial Endoscopic Evaluation of Esophageal Disease in A Cancer Model: A Paradigm Shift for Esophageal Adenocarcinoma (EAC) Drug Discovery and Development

Author

Ali H. Zaidi, Ashten N. Omstead, Haley Raphael, David A. Guel, Jenna Kolano, Patrick Campbell, Kevi Christopher, Matthew A. Smith, Ronan J. Kelly, Daisuke Matsui, Fahim Habib, Blair A. Jobe, Christopher K C Lai, Emily Smith, Samantha A. Martin, and Juliann E. Kosovec

Subjects
Oncology, medicine.medical_specialty, Hepatology, business.industry, Drug discovery, Esophageal disease, Cancer Model, Gastroenterology, Esophageal adenocarcinoma, medicine.disease, Internal medicine, Medicine, business
Published
2017
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